| A suite of simple tools for enabling bulk design of PCR primer sets to flank polymorphic features identified by NGS. Includes tools for parsing 454 and Illumina variant data into GFF3, identification of restriction polymorphisms and utilities to enable electronic PCR and primer mapping. |
hg clone https://toolshed.g2.bx.psu.edu/repos/john-mccallum/pcr_markers
| Name | Description | Version | Minimum Galaxy Version |
|---|---|---|---|
| In-Silico PCR Experiment Simulation System | 1.0.0 | any | |
| Design PCR Primers to Features | 1.0.0 | any | |
| Parse EMBOSS primer search output to tabular | 1.0.0 | any | |
| convert vcf to gvf/gff3 | 1.0.0 | any | |
| Convert output from In-Silico PCR Experiment Simulation System to GFF format | 1.0.0 | any | |
| Convert Roche gsMapper to GFF3 | 1.0.0 | any | |
| identify SNPs that condition restriction polymorphisms | 1.0.0 | any | |
| Extract unique feature IDs from a GVF/GFF3 file | 1.0.0 | any | |
| convert output of CAPS detection to gvf/gff3 | 1.0.0 | any | |
| convert output of patman pattern detection to gvf/gff3 | 1.0.0 | any | |
| Convert vcf to gff | 1.0.0 | any | |
| search for approximate patterns in DNA libraries | 1.0.0 | any | |