| SMAP design takes one or more reference sequences (FASTA and GFF) as input and designs non-overlapping amplicons per reference taking target specificity into account.
SMAP design can be combined with gRNA sequences for mutation induction of the reference sequences. As such SMAP design overlaps these amplicons and gRNAs, and selects n (user-defined) non-overlapping amplicons with gRNAs according several criteria such as number of gRNAs covered by the amplicon, specificity and efficiency scores. SMAP design creates a primer file, gRNA file, GFF file with all structural features, and optionally a summary file and plot, and input files required for downstream analysis using SMAP haplotype-window. |
hg clone https://toolshed.g2.bx.psu.edu/repos/ieguinoa/smap_design
| Name | Description | Version | Minimum Galaxy Version |
|---|---|---|---|
| 0.1.0 | 16.01 | ||