| Galaxy tool to filter FASTA, FASTQ or SFF sequences by SAM/BAM mapping
======================================================================
This tool is copyright 2014-2017 by Peter Cock, The James Hutton Institute
(formerly SCRI, Scottish Crop Research Institute), UK. All rights reserved.
See the licence text below.
This tool is a short Python script (using Biopython library functions) which
divides a FASTA, FASTQ, or SFF file in two, those sequences which do or do
not map according to given SAM/BAM file(s).
Example uses include mapping of FASTQ reads against a known contaminant
in order to remove reads prior to a de novo assembly.
This tool is available from the Galaxy Tool Shed at:
* http://toolshed.g2.bx.psu.edu/view/peterjc/seq_filter_by_mapping
See also related tools:
* http://toolshed.g2.bx.psu.edu/view/peterjc/seq_filter_by_id
* http://toolshed.g2.bx.psu.edu/view/peterjc/seq_select_by_id
* http://toolshed.g2.bx.psu.edu/view/peterjc/seq_rename
Automated Installation
======================
This should be straightforward using the Galaxy Tool Shed, which should be
able to automatically install the dependency on Biopython and samtools
and then install this tool and run its unit tests.
Manual Installation
===================
There are just two files to install to use this tool from within Galaxy:
* ``seq_filter_by_mapping.py`` (the Python script)
* ``seq_filter_by_mapping.xml`` (the Galaxy tool definition)
The suggested location is a dedicated ``tools/seq_filter_by_mapping/`` folder.
You will also need to modify the ``tools_conf.xml`` file to tell Galaxy to offer the
tool. One suggested location is in the filters section. Simply add the line::
If you wish to run the unit tests, also move/copy the ``test-data/`` files
under Galaxy's ``test-data/`` folder. Then::
$ ./run_tests.sh -id seq_filter_by_mapping
You will also need to install Biopython 1.54 or later. That's it.
History
=======
======= ======================================================================
Version Changes
------- ----------------------------------------------------------------------
v0.0.1 - Initial version.
v0.0.2 - Fixed some error messages.
v0.0.3 - Report counts for FASTQ as done for FASTA and SFF files.
v0.0.4 - Use the ``format_source=...`` tag.
- Reorder XML elements (internal change only).
- Planemo for Tool Shed upload (``.shed.yml``, internal change only).
v0.0.5 - Python script cleanups (internal change only).
- Depends on Biopython 1.67 via legacy Tool Shed package or bioconda.
- Use ```` (internal change only).
- Single quote command line arguments (internal change only).
v0.0.6 - Python 3 compatible print function.
v0.0.7 - Script works on Python 2 and 3 (fixed input file mode)
======= ======================================================================
Developers
==========
Development is on this GitHub repository:
https://github.com/peterjc/pico_galaxy/tree/master/tools/seq_filter_by_mapping
Much of the code was copied from my older tool:
https://github.com/peterjc/pico_galaxy/tree/master/tools/seq_filter_by_id
For pushing a release to the test or main "Galaxy Tool Shed", use the following
Planemo commands (which requires you have set your Tool Shed access details in
``~/.planemo.yml`` and that you have access rights on the Tool Shed)::
$ planemo shed_update -t testtoolshed --check_diff tools/seq_filter_by_mapping/
...
or::
$ planemo shed_update -t toolshed --check_diff tools/seq_filter_by_mapping/
...
To just build and check the tar ball, use::
$ planemo shed_upload --tar_only tools/seq_filter_by_mapping/
...
$ tar -tzf shed_upload.tar.gz
test-data/SRR639755_mito_pairs.fastq.gz
test-data/SRR639755_sample_by_coord.sam
test-data/SRR639755_sample_lax.fastq
test-data/SRR639755_sample_strict.fastq
tools/seq_filter_by_mapping/README.rst
tools/seq_filter_by_mapping/seq_filter_by_mapping.py
tools/seq_filter_by_mapping/seq_filter_by_mapping.xml
tools/seq_filter_by_mapping/tool_dependencies.xml
Licence (MIT)
=============
Permission is hereby granted, free of charge, to any person obtaining a copy
of this software and associated documentation files (the "Software"), to deal
in the Software without restriction, including without limitation the rights
to use, copy, modify, merge, publish, distribute, sublicense, and/or sell
copies of the Software, and to permit persons to whom the Software is
furnished to do so, subject to the following conditions:
The above copyright notice and this permission notice shall be included in
all copies or substantial portions of the Software.
THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR
IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY,
FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE
AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER
LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM,
OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN
THE SOFTWARE.
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