Grinder is a program to create random shotgun and amplicon sequence libraries based on reference sequences in a FASTA file. Features include:
* shotgun library or amplicon library * arbitrary read length distribution and number of reads * simulation of PCR and sequencing errors (chimeras, point mutations, homopolymers) * support for creating paired-end (mate pair) datasets * specific rank-abundance settings or manually given abundance for each genome * creation of datasets with a given richness (alpha diversity) * independent datasets can share a variable number of genomes (beta diversity) * modeling of the bias created by varying genome lengths or gene copy number * profile mechanism to store preferred options * API to automate the creation of a large number of simulated datasets |
hg clone https://toolshed.g2.bx.psu.edu/repos/fangly/grinder
Name | Description | Version | Minimum Galaxy Version |
---|---|---|---|
versatile omic shotgun and amplicon read simulator | 0.4.3 | any |