Processing DamID-seq data involves extending single-end reads, aligning the reads to the genome and determining the coverage, similar to processing regular ChIP-seq datasets. However, as DamID data is represented as a log2 ratio of (Dam-fusion/Dam), normalisation of the sample and Dam-only control is necessary and adding pseudocounts to mitigate the effect of background counts is highly recommended. |
hg clone https://toolshed.g2.bx.psu.edu/repos/mvdbeek/damidseq_polii_gene_call
Name | Description | Version | Minimum Galaxy Version |
---|---|---|---|
from DamIDseq data | 1.0.2 | 16.01 |