| Miscellaneous |
| Version lineage of this tool (guids ordered most recent to oldest) |
| toolshed.g2.bx.psu.edu/repos/iuc/trinity_analyze_diff_expr/trinity_analyze_diff_expr/2.15.1+galaxy0 (this tool) |
| toolshed.g2.bx.psu.edu/repos/iuc/trinity_analyze_diff_expr/trinity_analyze_diff_expr/2.9.1 |
| toolshed.g2.bx.psu.edu/repos/iuc/trinity_analyze_diff_expr/trinity_analyze_diff_expr/2.8.5+galaxy2 |
| toolshed.g2.bx.psu.edu/repos/iuc/trinity_analyze_diff_expr/trinity_analyze_diff_expr/2.8.5+galaxy1 |
| toolshed.g2.bx.psu.edu/repos/iuc/trinity_analyze_diff_expr/trinity_analyze_diff_expr/2.8.4+galaxy1 |
| toolshed.g2.bx.psu.edu/repos/iuc/trinity_analyze_diff_expr/trinity_analyze_diff_expr/2.8.4 |
| toolshed.g2.bx.psu.edu/repos/iuc/trinity_analyze_diff_expr/trinity_analyze_diff_expr/2.8.3 |
| toolshed.g2.bx.psu.edu/repos/iuc/trinity_analyze_diff_expr/trinity_analyze_diff_expr/2.6.6 |
| toolshed.g2.bx.psu.edu/repos/iuc/trinity_analyze_diff_expr/trinity_analyze_diff_expr/2.4.0.2 |
| toolshed.g2.bx.psu.edu/repos/iuc/trinity_analyze_diff_expr/trinity_analyze_diff_expr/2.4.0.0 |
| toolshed.g2.bx.psu.edu/repos/iuc/trinity_analyze_diff_expr/trinity_analyze_diff_expr/2.2.0.0 |
| trinity_analyze_diff_expr |
| Requirements (dependencies defined in the <requirements> tag set) |
| name | version | type |
| trinity | 2.15.1 | package |
| Additional information about this tool |
## DE results input files must be in the working directory and have suffix .DE_results
#import re
#for $input in $DE_results
#if re.search('.DE_results$',input.element_identifier)
## General case, where DE results files have been previously generated by run_de_analysis.pl
ln -s '${input}' "${re.sub('[^\w\-_.]', '_', input.element_identifier)}"
#else
## Particular case, where DE results files have non-standard names
ln -s '${input}' "${re.sub('[^\w\-_.]', '_', input.element_identifier)}.DE_results"
#end if
&&
#end for
#if str( $additional_params.GO_enrichment.examine_GO_enrichment ) == "yes":
## DE matrix input files must be in the working directory and have the same name as DE results input files, but replacing suffix .DE_results by suffix .count_matrix
#for $DE_matrix in $additional_params.GO_enrichment.DE_matrices
## Handle general case, where DE results files and DE matrix files have been previously generated by run_de_analysis.pl
ln -s '${DE_matrix}' "${re.sub('[^\w\-_.]', '_', DE_matrix.element_identifier)}"
&&
#end for
#end if
analyze_diff_expr.pl
--matrix '${matrix}'
--samples '${samples}'
-P ${P}
-C ${C}
#if str( $additional_params.max_DE_genes_per_comparison ):
--max_DE_genes_per_comparison ${additional_params.max_DE_genes_per_comparison}
#end if
$additional_params.order_columns_by_samples_file
#if $additional_params.max_genes_clust:
--max_genes_clust ${additional_params.max_genes_clust}
#end if
#if str( $additional_params.GO_enrichment.examine_GO_enrichment ) == "yes":
--examine_GO_enrichment
--GO_annots '${$additional_params.GO_enrichment.GO_annots}'
--gene_lengths '${$additional_params.GO_enrichment.gene_lengths}'
#end if
--output results
| Functional tests |
| name | inputs | outputs | required files |
| Test-1 |
matrix: count/qcheck/matrix.counts.matrix samples: count/samples.txt DE_results: list collection |
name: value |
count/qcheck/matrix.counts.matrix count/samples.txt count/exp_diff/input.matrix.wt_37_vs_wt_GSNO.DESeq2.DE_results count/exp_diff/input.matrix.wt_37_vs_wt_ph8.DESeq2.DE_results count/exp_diff/input.matrix.wt_GSNO_vs_wt_ph8.DESeq2.DE_results value |
| Test-2 |
matrix: count/qcheck/matrix.counts.matrix samples: count/samples.txt DE_results: list collection |
name: value |
count/qcheck/matrix.counts.matrix count/samples.txt count/exp_diff/input.matrix.wt_37_vs_wt_GSNO.DESeq2.DE_results count/exp_diff/input.matrix.wt_37_vs_wt_ph8.DESeq2.DE_results count/exp_diff/input.matrix.wt_GSNO_vs_wt_ph8.DESeq2.DE_results value |
| Test-3 |
matrix: count/qcheck/matrix.counts.matrix samples: count/samples.txt DE_results: list collection additional_params|GO_enrichment|DE_matrices: list collection additional_params|GO_enrichment|GO_annots: count/trinotate/go_annotations.txt additional_params|GO_enrichment|gene_lengths: count/trinotate/genes.lengths.txt additional_params|GO_enrichment|examine_GO_enrichment: yes |
count/qcheck/matrix.counts.matrix count/samples.txt count/exp_diff/input.matrix.wt_GSNO_vs_wt_ph8.DESeq2.DE_results count/exp_diff/input.matrix.wt_GSNO_vs_wt_ph8.DESeq2.count_matrix count/trinotate/go_annotations.txt count/trinotate/genes.lengths.txt |