Repository revision
2:2cd76b089570

Repository 'humann'
hg clone https://toolshed.g2.bx.psu.edu/repos/iuc/humann

HUMAnN tool metadata
Miscellaneous
HUMAnN
to profile presence/absence and abundance of microbial pathways and gene families
humann
toolshed.g2.bx.psu.edu/repos/iuc/humann/humann/3.6.0+galaxy0
3.6.0+galaxy0
humann --version
True
Version lineage of this tool (guids ordered most recent to oldest)
toolshed.g2.bx.psu.edu/repos/iuc/humann/humann/3.6.1+galaxy0
toolshed.g2.bx.psu.edu/repos/iuc/humann/humann/3.6.0+galaxy0 (this tool)
toolshed.g2.bx.psu.edu/repos/iuc/humann/humann/3.0.0+galaxy1
toolshed.g2.bx.psu.edu/repos/iuc/humann/humann/3.0.0+galaxy0
humann
Requirements (dependencies defined in the <requirements> tag set)
name version type
humann 3.6.0 package
Functional tests
name inputs outputs required files
Test-1 in|input: demo.fastq.gz
in|selector: raw
wf|prescreen|metaphlan_db|bowtie2db: test-db/metaphlan-db/demo-db-v30.fasta
wf|prescreen|metaphlan_db|mpa_pkl: test-db/metaphlan-db/old-structure/demo-db-v30.json
wf|prescreen|metaphlan_db|selector: history
wf|prescreen|prescreen_threshold: 0.01
wf|nucleotide_search|nucleotide_db|nucleotide_database: list collection
wf|nucleotide_search|nucleotide_db|selector: history
wf|selector: none
demo.fastq.gz
test-db/metaphlan-db/demo-db-v30.fasta
test-db/metaphlan-db/old-structure/demo-db-v30.json
test-db/nucleotide-db/g__Bacteroides.s__Bacteroides_dorei.centroids.v201901_v31.ffn.gz
test-db/nucleotide-db/g__Bacteroides.s__Bacteroides_vulgatus.centroids.v201901_v31.ffn.gz
Test-2 in|input: demo.fastq.gz
in|selector: raw
wf|prescreen|metaphlan_db|bowtie2db: test-db/metaphlan-db/demo-db-v30.fasta
wf|prescreen|metaphlan_db|mpa_pkl: test-db/metaphlan-db/demo-db-v30.json
wf|prescreen|metaphlan_db|selector: history
wf|prescreen|prescreen_threshold: 0.01
wf|nucleotide_search|nucleotide_db|nucleotide_database: list collection
wf|nucleotide_search|nucleotide_db|selector: history
wf|nucleotide_search|nucleotide_identity_threshold: 0
wf|nucleotide_search|nucleotide_subject_coverage_threshold: 50
wf|nucleotide_search|nucleotide_query_coverage_threshold: 90
wf|translated_search|protein_db|protein_database: test-db/protein-db/uniref90_demo_prots_v201901b.fasta
wf|translated_search|protein_db|search_mode: uniref90
wf|translated_search|protein_db|selector: history
wf|translated_search|evalue: 1
wf|translated_search|translated_subject_coverage_threshold: 50
wf|translated_search|translated_query_coverage_threshold: 90
wf|selector: none
g_p_quant|gap_fill: True
g_p_quant|minpath: True
g_p_quant|pathways: metacyc
g_p_quant|xipe: False
g_p_quant|annotation_gene_index: 3
out|output_basename: humann
out|output_format: tsv
out|output_max_decimals: 10
out|remove_column_description_output: False
out|intermediate_temp: ['metaphlan_bowtie2', 'metaphlan_bugs_list', 'bowtie2_alignment', 'bowtie2_reduced_alignment', 'bowtie2_unaligned', 'custom_chocophlan_database', 'diamond_aligned', 'diamond_unaligned']
name: value
name: value
name: value
name: value
name: value
name: value
name: value
name: value
name: value
name: value
name: value
name: value
demo.fastq.gz
test-db/metaphlan-db/demo-db-v30.fasta
test-db/metaphlan-db/demo-db-v30.json
test-db/nucleotide-db/g__Bacteroides.s__Bacteroides_dorei.centroids.v201901_v31.ffn.gz
test-db/nucleotide-db/g__Bacteroides.s__Bacteroides_vulgatus.centroids.v201901_v31.ffn.gz
test-db/protein-db/uniref90_demo_prots_v201901b.fasta
value
Test-3 in|input: demo.fasta.gz
in|selector: raw
wf|nucleotide_search|nucleotide_db|nucleotide_database: chocophlan-DEMO-20210421
wf|nucleotide_search|nucleotide_db|selector: cached
wf|nucleotide_search|nucleotide_identity_threshold: 0
wf|nucleotide_search|nucleotide_subject_coverage_threshold: 50
wf|nucleotide_search|nucleotide_query_coverage_threshold: 90
wf|translated_search|protein_db|protein_database: uniref-DEMO_diamond-20210421
wf|translated_search|protein_db|selector: cached
wf|translated_search|evalue: 1
wf|translated_search|translated_subject_coverage_threshold: 50
wf|translated_search|translated_query_coverage_threshold: 90
wf|selector: bypass_prescreen
g_p_quant|gap_fill: True
g_p_quant|minpath: True
g_p_quant|pathways: metacyc
g_p_quant|xipe: False
g_p_quant|annotation_gene_index: 3
out|output_basename: humann
out|output_format: biom
out|output_max_decimals: 10
out|remove_column_description_output: False
out|intermediate_temp:
name: value
name: value
name: value
name: value
demo.fasta.gz
value
Test-4 in|input: demo.fasta.gz
in|selector: raw
wf|taxonomic_profile: demo-taxonomic-profile.tabular
wf|nucleotide_search|nucleotide_db|nucleotide_database: chocophlan-DEMO-20210421
wf|nucleotide_search|nucleotide_db|selector: cached
wf|nucleotide_search|nucleotide_identity_threshold: 0
wf|nucleotide_search|nucleotide_subject_coverage_threshold: 50
wf|nucleotide_search|nucleotide_query_coverage_threshold: 90
wf|translated_search|protein_db|protein_database: uniref-DEMO_diamond-20210421
wf|translated_search|protein_db|selector: cached
wf|translated_search|evalue: 1
wf|translated_search|translated_subject_coverage_threshold: 50
wf|translated_search|translated_query_coverage_threshold: 90
wf|selector: bypass_taxonomic_profiling
g_p_quant|gap_fill: True
g_p_quant|minpath: True
g_p_quant|pathways: metacyc
g_p_quant|xipe: False
g_p_quant|annotation_gene_index: 3
out|output_basename: humann
out|output_format: tsv
out|output_max_decimals: 10
out|remove_column_description_output: False
out|intermediate_temp:
name: value
name: value
name: value
name: value
demo.fasta.gz
demo-taxonomic-profile.tabular
value
Test-5 in|input: demo.sam
in|selector: mapping
wf|nucleotide_search|nucleotide_db|nucleotide_database: chocophlan-DEMO-20210421
wf|nucleotide_search|nucleotide_db|selector: cached
wf|nucleotide_search|nucleotide_identity_threshold: 0
wf|nucleotide_search|nucleotide_subject_coverage_threshold: 50
wf|nucleotide_search|nucleotide_query_coverage_threshold: 90
wf|translated_search|protein_db|protein_database: uniref-DEMO_diamond-20210421
wf|translated_search|protein_db|selector: cached
wf|translated_search|evalue: 1
wf|translated_search|translated_subject_coverage_threshold: 50
wf|translated_search|translated_query_coverage_threshold: 90
wf|selector: bypass_nucleotide_index
g_p_quant|gap_fill: True
g_p_quant|minpath: True
g_p_quant|pathways: metacyc
g_p_quant|xipe: False
g_p_quant|annotation_gene_index: 3
out|output_basename: humann
out|output_format: tsv
out|output_max_decimals: 10
out|remove_column_description_output: False
out|intermediate_temp:
name: value
name: value
name: value
name: value
demo.sam
value
Test-6 in|input: demo.fastq.gz
in|selector: raw
wf|translated_search|protein_db|protein_database: uniref-DEMO_diamond-20210421
wf|translated_search|protein_db|selector: cached
wf|translated_search|evalue: 1
wf|translated_search|translated_subject_coverage_threshold: 50
wf|translated_search|translated_query_coverage_threshold: 90
wf|selector: bypass_nucleotide_search
g_p_quant|gap_fill: True
g_p_quant|minpath: True
g_p_quant|pathways: metacyc
g_p_quant|xipe: False
g_p_quant|annotation_gene_index: 3
out|output_basename: humann
out|output_format: tsv
out|output_max_decimals: 10
out|remove_column_description_output: False
out|intermediate_temp:
name: value
name: value
name: value
name: value
demo.fastq.gz
value
Test-7 in|input: demo.fastq.gz
in|selector: raw
wf|prescreen|metaphlan_db|cached_db: metaphlan-demo-db-20210421
wf|prescreen|metaphlan_db|selector: cached
wf|prescreen|prescreen_threshold: 0.01
wf|nucleotide_search|nucleotide_db|nucleotide_database: chocophlan-DEMO-20210421
wf|nucleotide_search|nucleotide_db|selector: cached
wf|nucleotide_search|nucleotide_identity_threshold: 0
wf|nucleotide_search|nucleotide_subject_coverage_threshold: 50
wf|nucleotide_search|nucleotide_query_coverage_threshold: 90
wf|selector: bypass_translated_search
g_p_quant|gap_fill: True
g_p_quant|minpath: True
g_p_quant|pathways: metacyc
g_p_quant|xipe: False
g_p_quant|annotation_gene_index: 3
out|output_basename: newname
out|output_format: tsv
out|output_max_decimals: 10
out|remove_column_description_output: False
out|intermediate_temp:
name: value
name: value
name: value
name: value
demo.fastq.gz
value
Test-8 in|input: demo.fastq.gz
in|selector: raw
wf|prescreen|metaphlan_db|cached_db: metaphlan-db-old-structure
wf|prescreen|metaphlan_db|selector: cached
wf|nucleotide_search|nucleotide_db|nucleotide_database: chocophlan-DEMO-20210421
wf|nucleotide_search|nucleotide_db|selector: cached
wf|selector: bypass_translated_search
demo.fastq.gz