Miscellaneous |
Version lineage of this tool (guids ordered most recent to oldest) |
toolshed.g2.bx.psu.edu/repos/iuc/megan_sam2rma/megan_sam2rma/6.21.7+galaxy0 (this tool) |
megan_sam2rma |
Requirements (dependencies defined in the <requirements> tag set) |
name | version | type |
megan | 6.21.7 | package |
Additional information about this tool |
#import re #if str($input_type_cond.input_type) in ['single', 'pair']: #set read1 = $input_type_cond.read1 #set sam1 = $input_type_cond.sam1 #else: ## Processing paired reads are tricky if we're ## downstream from MALT. MALT doesn’t have a ## paired-read mode, so it won’t attempt to analyze ## reads in pairs. To do paired read processing, ## set MALT to generate SAM files and then import the ## SAM files into MEGAN, specifying paired read mode ## there. If you have multiple SAM files for the same ## sample, then import them all at the same time to ## create one unified rma6 file. #set read1 = $input_type_cond.reads_collection['forward'] #set sam1 = $input_type_cond.sam1 #end if #if $read1.is_of_type('fasta', 'fasta.gz'): #set read_ext = '.fasta' #else: #set read_ext = '.fastq' #end if #if $read1.ext.endswith('.gz'): #set read_ext = $read_ext + '.gz' #end if #set read1_identifier = 'read1' + $read_ext ln -s '${read1}' '${read1_identifier}' && #set sam1_identifier = 'sam1.' + $sam1.ext ln -s '${sam1}' '${sam1_identifier}' && #if str($input_type_cond.input_type) in ['pair', 'paired']: #if str($input_type_cond.input_type) == 'pair': #set read2 = $input_type_cond.read2 #set sam2 = $input_type_cond.sam2 #else if str($input_type_cond.input_type) == 'paired': #set read2 = $input_type_cond.reads_collection['reverse'] #set sam2 = $input_type_cond.sam2 #end if #set read2_identifier = 'read2' + $read_ext ln -s '${read2}' '${read2_identifier}' && #set sam2_identifier = 'sam2.' + $sam2.ext ln -s '${sam2}' '${sam2_identifier}' && #end if ## The output must be a directory when we have multiple ## inputs, and the outputs inherit the base name of the ## inputs. sam2rma #if str($input_type_cond.input_type) == 'single': --in '${sam1_identifier}' --reads '${read1_identifier}' --out '${output_single}' #else if str($input_type_cond.input_type) == 'pair': --in '${sam1_identifier}' '${sam2_identifier}' --reads '${read1_identifier}' '${read2_identifier}' --paired --pairedSuffixLength $input_type_cond.pairedSuffixLength --out '.' #else if str($input_type_cond.input_type) == 'paired': --in '${sam1_identifier}' '${sam2_identifier}' --reads '${read1_identifier}' '${read2_identifier}' --paired --pairedSuffixLength $input_type_cond.pairedSuffixLength ## Strangely, megan requires an output ## directory when processing paired reads ## even though it produces a single file. ## We'll accommodate thie by prepending ./ ## to a temporary output file and then move ## it later. --out '.' #end if #if $advanced_options.metaDataFile: --metaDataFile '$advanced_options.metaDataFile' #end if #if str($advanced_options.paired_reads_cond.paired_reads) == 'yes': --paired $advanced_options.paired_reads_cond.pairedSuffixLength #end if $advanced_options.longReads --maxMatchesPerRead $advanced_options.maxMatchesPerRead $advanced_options.classify --minScore $advanced_options.minScore --maxExpected $advanced_options.maxExpected --topPercent $advanced_options.topPercent --minSupportPercent $advanced_options.minSupportPercent --minSupport $advanced_options.minSupport --minPercentReadCover $advanced_options.minPercentReadCover --minPercentReferenceCover $advanced_options.minPercentReferenceCover --minReadLength $advanced_options.minReadLength --lcaAlgorithm '$advanced_options.lcaAlgorithm' --lcaCoveragePercent $advanced_options.lcaCoveragePercent --readAssignmentMode '$advanced_options.readAssignmentMode' #if $advanced_options.conFile: --conFile '$advanced_options.conFile' #end if #if $advanced_options.mapDB: --mapDB '$advanced_options.mapDB' #end if #if str($advanced_options.only) != '': --only '$advanced_options.only' #end if --useCompression 'false' --threads \${GALAXY_SLOTS:-8} --tempStoreDir '.' #if str($input_type_cond.input_type) in ['pair', 'paired']: && mv 'sam1.rma6' '$output_forward' && mv 'sam2.rma6' '$output_reverse' #end if
Functional tests |
name | inputs | outputs | required files |
Test-1 |
input_type_cond|read1: 13-1941-6_S4_L001_R1_600000.fastq.gz input_type_cond|sam1: input1.sam |
name: value |
13-1941-6_S4_L001_R1_600000.fastq.gz input1.sam value |
Test-2 |
input_type_cond|read1: 13-1941-6_S4_L001_R1_600000.fastq.gz input_type_cond|sam1: input1.sam input_type_cond|read2: 13-1941-6_S4_L001_R2_600000.fastq.gz input_type_cond|sam2: input2.sam input_type_cond|input_type: pair |
name: value name: value |
13-1941-6_S4_L001_R1_600000.fastq.gz input1.sam 13-1941-6_S4_L001_R2_600000.fastq.gz input2.sam value |
Test-3 |
input_type_cond|reads_collection: paired collection input_type_cond|sam1: input1.sam input_type_cond|sam2: input2.sam input_type_cond|input_type: paired |
name: value name: value |
13-1941-6_S4_L001_R1_600000.fastq.gz 13-1941-6_S4_L001_R2_600000.fastq.gz input1.sam input2.sam value |