| Miscellaneous |
| Version lineage of this tool (guids ordered most recent to oldest) |
| toolshed.g2.bx.psu.edu/repos/devteam/picard/picard_AddOrReplaceReadGroups/3.1.1.0 (this tool) |
| toolshed.g2.bx.psu.edu/repos/devteam/picard/picard_AddOrReplaceReadGroups/1.126.0 |
| picard_AddOrReplaceReadGroups |
| Requirements (dependencies defined in the <requirements> tag set) |
| name | version | type |
| picard | 3.1.1 | package |
| Additional information about this tool |
#def identifier_or_name($input1)
#if hasattr($input1, 'element_identifier')
#return $input1.element_identifier
#else
#return $input1.name.rstrip('.gz').rstrip('.fastq').rstrip('.fq')
#end if
#end def
#def clean(name)
#import re
#set $name_clean = re.sub('[^\w\-_\.]', '_', $name)
#return $name_clean
#end def
#def read_group_name_default($input1, $input2=None)
#if $input2 is None
#return $clean($identifier_or_name($input1))
#else
#import itertools
#set $input_name1 = $clean($identifier_or_name($input1))
#set $input_name2 = $clean($identifier_or_name($input2))
#set $common_prefix = ''.join([c[0] for c in itertools.takewhile(lambda x: all(x[0] == y for y in x), zip(*[$input_name1, $input_name2]))])
#if len($common_prefix) > 3
#return $common_prefix
#else
#return $input_name1
#end if
#end if
#end def
#def format_read_group(prefix, value, quote='', arg='')
#if $value
#return $arg + $quote + $prefix + $value + $quote
#else
#return ''
#end if
#end def
#def rg_param(name)
#if $varExists("rg")
#return $rg.get($name, None)
#else
#return $getVar($name, None)
#end if
#end def
#set $use_rg = True
#set $rg_auto_name = $read_group_name_default($inputFile)
#if $use_rg
#if $rg_param('read_group_id_conditional') is None
#set $rg_id = $rg_auto_name
#elif $rg_param('read_group_id_conditional').do_auto_name
#set $rg_id = $rg_auto_name
#else
#set $rg_id = str($rg_param('read_group_id_conditional').ID)
#end if
#if $rg_param('read_group_sm_conditional') is None
#set $rg_sm = ''
#elif $rg_param('read_group_sm_conditional').do_auto_name
#set $rg_sm = $rg_auto_name
#else
#set $rg_sm = str($rg_param('read_group_sm_conditional').SM)
#end if
#if $rg_param('PL')
#set $rg_pl = str($rg_param('PL'))
#else
#set $rg_pl = ''
#end if
#if $rg_param('read_group_lb_conditional') is None
#set $rg_lb = ''
#elif $rg_param('read_group_lb_conditional').do_auto_name
#set $rg_lb = $rg_auto_name
#else
#set $rg_lb = str($rg_param('read_group_lb_conditional').LB)
#end if
#if $rg_param('CN')
#set $rg_cn = str($rg_param('CN'))
#else
#set $rg_cn = ''
#end if
#if $rg_param("DS")
#set $rg_ds = str($rg_param("DS"))
#else
#set $rg_ds = ''
#end if
#if $rg_param("DT")
#set $rg_dt = str($rg_param("DT"))
#else
#set $rg_dt = ''
#end if
#if $rg_param("FO")
#set $rg_fo = str($rg_param("FO"))
#else
#set $rg_fo = ''
#end if
#if $rg_param("KS")
#set $rg_ks = str($rg_param("KS"))
#else
#set $rg_ks = ''
#end if
#if $rg_param("PG")
#set $rg_pg = str($rg_param("PG"))
#else
#set $rg_pg = ''
#end if
#if $rg_param("PI") != None
#set $rg_pi = str($rg_param("PI"))
#else
#set $rg_pi = ''
#end if
#if $rg_param("PU")
#set $rg_pu = str($rg_param("PU"))
#else
#set $rg_pu = ''
#end if
#end if
_JAVA_OPTIONS=\${_JAVA_OPTIONS:-"-Xmx2048m -Xms256m -Djava.io.tmpdir=\${TMPDIR:-\${_GALAXY_JOB_TMPDIR}}"} &&
export _JAVA_OPTIONS &&
#import re
#set escaped_element_identifier = re.sub('[^\w\-]', '_', str($inputFile.element_identifier))
ln -sf '$inputFile' '$escaped_element_identifier' &&
picard AddOrReplaceReadGroups
--INPUT '$escaped_element_identifier'
$format_read_group("", $rg_lb, quote='"', arg=" --RGLB ")
$format_read_group("", $rg_pl, quote='"', arg=" --RGPL ")
$format_read_group("", $rg_pu, quote='"', arg=" --RGPU ")
$format_read_group("", $rg_sm, quote='"', arg=" --RGSM ")
$format_read_group("", $rg_id, quote='"', arg=" --RGID ")
$format_read_group("", $rg_ds, quote='"', arg=" --RGDS ")
$format_read_group("", $rg_pi, quote='"', arg=" --RGPI ")
$format_read_group("", $rg_dt, quote='"', arg=" --RGDT ")
--OUTPUT '${outFile}'
--VALIDATION_STRINGENCY '${validation_stringency}'
--QUIET true
--VERBOSITY ERROR
| Functional tests |
| name | inputs | outputs | required files |
| Test-1 |
inputFile: picard_ARRG.bam read_group_id_conditional|ID: id-1 read_group_sm_conditional|SM: sample-a read_group_lb_conditional|LB: tumor-a PL: ILLUMINA PU: run-1 |
name: value |
picard_ARRG.bam value |