Miscellaneous |
Version lineage of this tool (guids ordered most recent to oldest) |
toolshed.g2.bx.psu.edu/repos/iuc/sra_tools/fasterq_dump/3.0.3+galaxy0 |
toolshed.g2.bx.psu.edu/repos/iuc/sra_tools/fasterq_dump/3.0.0+galaxy0 (this tool) |
toolshed.g2.bx.psu.edu/repos/iuc/sra_tools/fasterq_dump/2.11.0+galaxy1 |
toolshed.g2.bx.psu.edu/repos/iuc/sra_tools/fasterq_dump/2.11.0+galaxy0 |
toolshed.g2.bx.psu.edu/repos/iuc/sra_tools/fasterq_dump/2.10.9+galaxy0 |
toolshed.g2.bx.psu.edu/repos/iuc/sra_tools/fasterq_dump/2.10.8+galaxy0 |
toolshed.g2.bx.psu.edu/repos/iuc/sra_tools/fasterq_dump/2.10.7+galaxy2 |
toolshed.g2.bx.psu.edu/repos/iuc/sra_tools/fasterq_dump/2.10.7+galaxy1 |
toolshed.g2.bx.psu.edu/repos/iuc/sra_tools/fasterq_dump/2.10.7+galaxy2 |
toolshed.g2.bx.psu.edu/repos/iuc/sra_tools/fasterq_dump/2.10.7+galaxy1 |
toolshed.g2.bx.psu.edu/repos/iuc/sra_tools/fasterq_dump/2.10.7+galaxy0 |
toolshed.g2.bx.psu.edu/repos/iuc/sra_tools/fasterq_dump/2.10.4+galaxy2 |
toolshed.g2.bx.psu.edu/repos/iuc/sra_tools/fasterq_dump/2.10.4+galaxy1 |
toolshed.g2.bx.psu.edu/repos/iuc/sra_tools/fasterq_dump/2.10.4 |
fasterq_dump |
Requirements (dependencies defined in the <requirements> tag set) |
name | version | type |
sra-tools | 3.0.0 | package |
pigz | 2.6 | package |
samtools | 1.16.1 | package |
Additional information about this tool |
set -o | grep -q pipefail && set -o pipefail; mkdir -p ~/.ncbi && cp '$user_settings_mkfg' ~/.ncbi/user-settings.mkfg && vdb-config -s "/repository/user/main/public/root=\$PWD" && vdb-config -s "/repository/user/ad/public/root=\$PWD" && vdb-config -s "/repository/user/default-path=\$PWD" && vdb-config -s "/repository/user/main/public/root=\$PWD" && vdb-config -s /http/timeout/read=10000 && export SRA_PREFETCH_RETRIES=3 && export SRA_PREFETCH_ATTEMPT=1 && #if $input.input_select == "sra_file": acc='${input.sra_file.name}' && ln -s '${input.sra_file}' "\$acc" && #else #if $input.input_select == "file_list": #if $input.file_list.is_of_type('sra_manifest.tabular'): #set $column = $input.file_list.unsanitized.metadata.column_names.index('Run') + 1 cut -f $column '$input.file_list'| tail -n +2 > accessions && #else grep '^[[:space:]]*[E|S|D]RR[0-9]\{1,\}[[:space:]]*$' '$input.file_list' > accessions && #end if #elif $input.input_select == "accession_number": echo '${input.accession}' | sed -r 's/(\,|\;|__cn__)/\n/g' > accessions && #end if for acc in \$(cat ./accessions); do ( echo "Downloading accession: \$acc..." && #end if while [ \$SRA_PREFETCH_ATTEMPT -le \$SRA_PREFETCH_RETRIES ] ; do fasterq-dump "\$acc" -e \${GALAXY_SLOTS:-1} --seq-defline '@\$sn/\$ri' --qual-defline '+' $adv.split #if str( $adv.minlen ) != "": --min-read-len "$adv.minlen" #end if $adv.skip_technical 2>&1 | tee -a '$log'; if [ \$? == 0 ] && [ \$(ls *.fastq | wc -l) -ge 1 ]; then break ; else echo "Prefetch attempt \$SRA_PREFETCH_ATTEMPT of \$SRA_PREFETCH_RETRIES exited with code \$?" ; SRA_PREFETCH_ATTEMPT=`expr \$SRA_PREFETCH_ATTEMPT + 1` ; sleep 1 ; fi ; done && mkdir -p output && mkdir -p outputOther && count="\$(ls *.fastq | wc -l)" && echo "There are \$count fastq files" && data=(\$(ls *.fastq)) && if [ "\$count" -eq 1 ]; then pigz -cqp \${GALAXY_SLOTS:-1} "\${data[0]}" > output/"\${acc}"__single.fastqsanger.gz && rm "\${data[0]}"; elif [ "$adv.split" = "--split-3" ]; then if [ -e "\${acc}".fastq ]; then pigz -cqp \${GALAXY_SLOTS:-1} "\${acc}".fastq > outputOther/"\${acc}"__single.fastqsanger.gz; fi && pigz -cqp \${GALAXY_SLOTS:-1} "\${acc}"_1.fastq > output/"\${acc}"_forward.fastqsanger.gz && pigz -cqp \${GALAXY_SLOTS:-1} "\${acc}"_2.fastq > output/"\${acc}"_reverse.fastqsanger.gz && rm "\${acc}"*.fastq; elif [ "\$count" -eq 2 ]; then #if $adv.skip_technical: pigz -cqp \${GALAXY_SLOTS:-1} "\${data[0]}" > output/"\${acc}"_forward.fastqsanger.gz && pigz -cqp \${GALAXY_SLOTS:-1} "\${data[1]}" > output/"\${acc}"_reverse.fastqsanger.gz && #else pigz -cqp \${GALAXY_SLOTS:-1} "\${data[0]}" > outputOther/"\${data[0]}"sanger.gz && pigz -cqp \${GALAXY_SLOTS:-1} "\${data[1]}" > outputOther/"\${data[1]}"sanger.gz && #end if rm "\${data[0]}" && rm "\${data[1]}"; else for file in \${data[*]}; do pigz -cqp \${GALAXY_SLOTS:-1} "\$file" > outputOther/"\$file"sanger.gz && rm "\$file"; done; fi; #if $input.input_select != "sra_file": ); done; #end if echo "Done with all accessions."
Functional tests |
name | inputs | outputs | required files |
Test-1 |
input|accession: ERR086330 input|input_select: accession_number |
||
Test-2 |
input|accession: SRR002702 input|input_select: accession_number adv|split: --split-files adv|skip_technical: False |
||
Test-3 |
input|accession: ERR086330, SRR11953971 input|input_select: accession_number |
||
Test-4 |
input|sra_file: SRR522874.sra input|input_select: sra_file adv|split: --split-files adv|skip_technical: True |
SRR522874.sra |
|
Test-5 |
input|sra_file: SRR522874.sra input|input_select: sra_file adv|split: --split-files adv|skip_technical: False |
SRR522874.sra |
|
Test-6 |
input|file_list: list_sra input|input_select: file_list adv|minlen: 21 |
list_sra |
|
Test-7 |
input|file_list: sra_manifest.tabular input|input_select: file_list |
sra_manifest.tabular |