Miscellaneous |
Version lineage of this tool (guids ordered most recent to oldest) |
toolshed.g2.bx.psu.edu/repos/iuc/umi_tools_extract/umi_tools_extract/1.1.2+galaxy2 (this tool) |
toolshed.g2.bx.psu.edu/repos/iuc/umi_tools_extract/umi_tools_extract/1.1.2+galaxy1 |
toolshed.g2.bx.psu.edu/repos/iuc/umi_tools_extract/umi_tools_extract/1.1.2+galaxy0 |
toolshed.g2.bx.psu.edu/repos/iuc/umi_tools_extract/umi_tools_extract/0.5.5.2 |
toolshed.g2.bx.psu.edu/repos/iuc/umi_tools_extract/umi_tools_extract/0.5.5.1 |
toolshed.g2.bx.psu.edu/repos/iuc/umi_tools_extract/umi_tools_extract/0.5.5.0 |
toolshed.g2.bx.psu.edu/repos/iuc/umi_tools_extract/umi_tools_extract/0.5.3.2 |
toolshed.g2.bx.psu.edu/repos/iuc/umi_tools_extract/umi_tools_extract/0.5.3.1 |
toolshed.g2.bx.psu.edu/repos/iuc/umi_tools_extract/umi_tools_extract/0.5.3.0 |
toolshed.g2.bx.psu.edu/repos/iuc/umi_tools_extract/umi_tools_extract/0.5.0.0 |
toolshed.g2.bx.psu.edu/repos/iuc/umi_tools_extract/umi_tools_extract/0.4.4.0 |
umi_tools_extract |
Requirements (dependencies defined in the <requirements> tag set) |
name | version | type |
umi_tools | 1.1.2 | package |
Additional information about this tool |
#set $gz = False #if $input_type_cond.input_type == 'single': #if $input_type_cond.input_read1.is_of_type("fastq.gz", "fastqsanger.gz"): ln -s '$input_type_cond.input_read1' input_single.gz && #set $gz = True #else ln -s '$input_type_cond.input_read1' input_single.txt && #end if #elif $input_type_cond.input_type == 'paired': #if $input_type_cond.input_read1.is_of_type("fastq.gz", "fastqsanger.gz"): ln -s '$input_type_cond.input_read1' input_read1.gz && ln -s '$input_type_cond.input_read2' input_read2.gz && #set $gz = True #else ln -s '$input_type_cond.input_read1' input_read1.txt && ln -s '$input_type_cond.input_read2' input_read2.txt && #end if #else ## paired_collection #if $input_type_cond.input_readpair.forward.is_of_type("fastq.gz", "fastqsanger.gz"): ln -s '$input_type_cond.input_readpair.forward' input_read1.gz && ln -s '$input_type_cond.input_readpair.reverse' input_read2.gz && #set $gz = True #else ln -s '$input_type_cond.input_readpair.forward' input_read1.txt && ln -s '$input_type_cond.input_readpair.reverse' input_read2.txt && #end if #end if umi_tools extract ## fastq barcode extraction options: --extract-method='$extract_method_cond.extract_method' --bc-pattern='$input_type_cond.bc_pattern' #if $input_type_cond.input_type != 'single' and $input_type_cond.bc_pattern2 != '' --bc-pattern2='$input_type_cond.bc_pattern2' #end if #if $extract_method_cond.extract_method == 'string' $extract_method_cond.prime3 #else if $extract_method_cond.filtered_out_bool #if $input_type_cond.input_type == 'single': --filtered-out='$filtered_out' #else if $input_type_cond.input_type == 'paired': --filtered-out='$filtered_out' --filtered-out2='$filtered_out_paired' #else --filtered-out='$filtered_out_paired_collection.forward' --filtered-out2='$filtered_out_paired_collection.reverse' #end if #end if $ignore_read_pair_suffixes #if $input_type_cond.input_type == 'single': #if $gz: --stdin=input_single.gz --stdout out.gz #else --stdin=input_single.txt --stdout '$out' #end if #else: #if $gz: --stdin=input_read1.gz --read2-in=input_read2.gz --stdout out1.gz --read2-out=out2.gz #else: --stdin=input_read1.txt --read2-in=input_read2.txt #if $input_type_cond.input_type == 'paired' --stdout '$out' --read2-out='$out2' #else --stdout '$out_paired_collection.forward' --read2-out='$out_paired_collection.reverse' #end if #end if $input_type_cond.reconcile_pairs #end if #if $whitelist --whitelist='$whitelist' #end if #if $blacklist --blacklist='$blacklist' #end if $error_correct_cell.value #if $quality.quality_selector =='true': #if str($quality.quality_filter_threshold) != '' --quality-filter-threshold '$quality.quality_filter_threshold' #end if #if str($quality.quality_filter_mask) != '' --quality-filter-mask '$quality.quality_filter_mask' #end if #if $input_type_cond.input_type != 'paired_collection' #set input=$input_type_cond.input_read1 #else #set input=$input_type_cond.input_readpair.forward #end if --quality-encoding #if $input.ext.startswith("fastqillumina") phred64 #else if $input.ext.startswith("fastqsolexa") solexa #else phred33 #end if #end if #if $log: --log='$out_log' #end if --log2stderr #if $gz: #if $input_type_cond.input_type == 'single': && mv out.gz '$out' #else if $input_type_cond.input_type == 'paired' && mv out1.gz '$out' && mv out2.gz '$out2' #else && mv out1.gz '$out_paired_collection.forward' && mv out2.gz '$out_paired_collection.reverse' #end if #end if
Functional tests |
name | inputs | outputs | required files |
Test-1 |
input_type_cond|input_read1: t_R1.fastq input_type_cond|bc_pattern: XXXNNN input_type_cond|input_type: single extract_method_cond|prime3: True quality|quality_filter_threshold: 10 quality|quality_selector: true log: True |
name: value name: value |
t_R1.fastq value |
Test-2 |
input_type_cond|input_read1: t_R1.fastq.gz input_type_cond|input_read2: t_R2.fastq.gz input_type_cond|bc_pattern: NNNXXX input_type_cond|input_type: paired log: True |
name: value name: value name: value |
t_R1.fastq.gz t_R2.fastq.gz value |
Test-3 |
input_type_cond|input_readpair: paired collection input_type_cond|bc_pattern: NNNXXX input_type_cond|input_type: paired_collection log: True |
name: value |
t_R1.fastq t_R2.fastq value |
Test-4 |
input_type_cond|input_read1: scrb_seq_fastq.1.gz input_type_cond|input_read2: scrb_seq_fastq.2.gz input_type_cond|bc_pattern: CCCCCCNNNNNNNNNN input_type_cond|input_type: paired extract_method_cond|extract_method: string whitelist: scrb_seq_barcodes log: True |
name: value |
scrb_seq_fastq.1.gz scrb_seq_fastq.2.gz scrb_seq_barcodes value |
Test-5 |
input_type_cond|input_read1: scrb_seq_fastq.1.gz input_type_cond|input_read2: scrb_seq_fastq.2.gz input_type_cond|bc_pattern: ^(?P<cell_1>.{6})(?P<umi_1>.{10}) input_type_cond|input_type: paired extract_method_cond|extract_method: regex whitelist: scrb_seq_barcodes log: True |
name: value |
scrb_seq_fastq.1.gz scrb_seq_fastq.2.gz scrb_seq_barcodes value |
Test-6 |
input_type_cond|input_read1: read_R1.200.gz input_type_cond|input_read2: read_R2.200.gz input_type_cond|bc_pattern: NNNNNNCCCCCC input_type_cond|input_type: paired extract_method_cond|extract_method: string |
name: value name: value |
read_R1.200.gz read_R2.200.gz value |