view rank_terms.xml @ 39:e56023008e36 default tip

Changed revision of package_fisher_0_1_4 to be2fc454d121 Changed revision of package_matplotlib_1_2 to a03ee94316b5

<tool id="gd_rank_terms" name="Rank Terms" version="1.1.0">
  <description>: Assess the enrichment/depletion of a gene set for GO terms</description>

  <command interpreter="python">
    #set $t_col1_0 = int(str($t_col1)) - 1
    #set $t_col2_0 = int(str($t_col2)) - 1
    #set $g_col2_0 = int(str($g_col2)) - 1
    rank_terms.py --input "$input1" --columnENSEMBLT $t_col1_0 --inExtnddfile "$input2" --columnENSEMBLTExtndd $t_col2_0 --columnGOExtndd $g_col2_0 --statsTest "$stat" --output "$output"
  </command>

  <inputs>
    <param name="input1" type="data" format="tabular" label="Query dataset" />
    <param name="t_col1" type="data_column" data_ref="input1" label="Column with ENSEMBL transcript codes" />
    <param name="input2" type="data" format="tabular" label="Background dataset" />
    <param name="t_col2" type="data_column" data_ref="input2" label="Column with ENSEMBL transcript codes" />
    <param name="g_col2" type="data_column" data_ref="input2" label="Column with GO terms" />
    <param name="stat" type="select" label="Statistic for determining enrichment/depletion">
      <option value="fisher" selected="true">two-tailed Fisher's exact test</option>
      <option value="hypergeometric">hypergeometric test</option>
      <option value="binomial">binomial probability</option>
    </param>
  </inputs>

  <outputs>
    <data name="output" format="tabular" />
  </outputs>

  <requirements>
    <requirement type="package" version="0.1.4">fisher</requirement>
  </requirements>

  <help>

**Dataset formats**

All of the input and output datasets are in tabular_ format.
The query dataset has a column containing ENSEMBL transcript codes for
the gene set of interest, while the background dataset has one column
with ENSEMBL transcript codes and another with GO terms, for some
larger universe of genes.
The output dataset is described below.
(`Dataset missing?`_)

.. _tabular: ./static/formatHelp.html#tab
.. _Dataset missing?: ./static/formatHelp.html

-----

**What it does**

Given a query set of genes from a larger background dataset, this tool
evaluates the over- or under-representation of Gene Ontology terms in the
query set, using the specified statistical test.

The output contains a row for each GO term, with the following columns:

1. count: the number of genes in the query set that are in this GO category
2. representation: the percentage of this category's genes (from the background dataset) that appear in the query set
3. ranking of this term, based on its representation ("1" is highest)
4. probability of depletion of this GO category in the query dataset
5. probability of enrichment of this GO category in the query dataset
6. GO term

  </help>
</tool>