view trinity.xml @ 8:f6fff1488aa8 draft

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<tool id="trinityrnaseq" name="Trinity" version="r20140717">

    <!-- Written by Jeremy Goecks -->
    <!-- Modified by Anmol J. Hemrom -->

    <description>De novo assembly of RNA-Seq data Using Trinity</description>
    <requirements>
        <requirement type="package" version="r20140717">trinityrnaseq</requirement>
        <requirement type="package" version="0.12.7">bowtie</requirement>
        <requirement type="package" version="1.2">samtools</requirement>  
    </requirements>
    <command>
        Trinity --JM $JM --CPU $CPU
        
        ## Inputs.
        #if str($inputs.paired_or_single) == "paired":
            --left $inputs.left_input --right $inputs.right_input
            #if  $inputs.left_input.ext == 'fa':
                --seqType fa
            #else:
                --seqType fq
            #end if
            #if str($inputs.library_type) != "None":
                --SS_lib_type $inputs.library_type
            #end if
--group_pairs_distance $inputs.group_pairs_distance
        #else:
            --single $inputs.input
            #if  str($inputs.input.ext) == 'fa':
                --seqType fa
            #else:
                --seqType fq
            #end if
            #if str($inputs.library_type) != "None":
                --SS_lib_type $inputs.library_type
            #end if
        #end if

        ## Additional parameters.
        #if str($additional_params.use_additional) == "yes":
--min_kmer_cov $inputs.min_kmer_cov --max_reads_per_graph $inputs.max_reads_per_graph --bflyHeapSpaceMax $input.bflyHeapSpaceMax
#if $inputs.bfly_opts != 'None':
--bfly_opts " $inputs.bfly_opts "
#end if
        #end if

                
        ## direct to output
        > $trinity_log 2>&amp;1
 
    </command>
    <inputs>
        <param name="JM" type="select" label="JM" help="Amount of memory to allocate to Jellyfish for Kmer catalog construction">
<option value="1G">1G</option>
<option value="10G">10G</option>
<option value="50G">50G</option>
<option value="100G">100G</option>
<option value="200G">200G</option>
<option value="500G">500G</option>
</param>

<param name="CPU" type="integer" value="2" min="1" label="CPU" help="Number of CPUs to use by Trinity" />


        <conditional name="inputs">
    <param name="paired_or_single" type="select" label="Paired or Single-end data?">
                <option value="paired">Paired</option>
                <option value="single">Single</option>
            </param>
            <when value="paired">
                <param format="fasta,fastq" name="left_input" type="data" label="Left/Forward strand reads" help=""/>
                <param format="fasta,fastq" name="right_input" type="data" label="Right/Reverse strand reads" help=""/>
                <param name="library_type" type="select" label="Strand-specific Library Type">
                    <option value="None">None</option>
                    <option value="FR">FR</option>
                    <option value="RF">RF</option>
                </param>
                <param name="group_pairs_distance" type="integer" value="500" min="1" label="Group pairs distance" help="Maximum length expected between fragment pairs"/>
        <param name="path_reinforcement_distance" type="integer" value="75" min="1" label="Path reinforcement distance" help="Minimum read overlap required for path extension in the graph" />    

            </when>
            <when value="single">
                <param format="fasta,fastq" name="input" type="data" label="Single-end reads" help=""/>
                <param name="library_type" type="select" label="Strand-specific Library Type">
                    <option value="None">None</option>
                    <option value="F">F</option>
                    <option value="R">R</option>
                </param>
            <param name="path_reinforcement_distance" type="integer" value="40" min="1" label="Path reinforcement distance" help="Minimum read overlap required for path extension in the graph" />    
            </when>
        </conditional>
        
<conditional name="additional_params">
            <param name="use_additional" type="select" label="Use Additional Params?">
                <option value="no">No</option>
                <option value="yes">Yes</option>
            </param>
            <when value="no">
            </when>
            <when value="yes">            
<param name="min_kmer_cov" type="integer" value="1" min="1" label="inchworm_min_kmer_cov" help="Minimum kmer coverage required by Inchworm for initial contig construction" />
<param name="max_reads_per_graph" type="integer" value="20000000" min="10000" label="chrysalis_max_reads_per_graph" help="Maximum number of reads to be anchored within each transcript graph by Chrysalis" />


<param name="bfly_opts" type="text" value="None" label="bfly_opts" help="Options to pass on to Butterfly" />
<param name="bflyHeapSpaceMax" type="select" label="bflyHeapSpaceMax" help="Java heap space maximum value for Butterfly">
<option value="1G">1G</option>
<option value="2G">2G</option>
<option value="4G" selected="true">4G</option>
<option value="10G">10G</option>
<option value="20G">20G</option>
</param>

               <param name="min_contig_length" type="integer" value="200" min="1" label="Minimum Contig Length" help=""/>
            </when>
        </conditional>
    

    </inputs>
    <outputs>
        <data format="txt" name="trinity_log" label="${tool.name} on ${on_string}: log" />
        <data format="fasta" name="assembled_transcripts" label="${tool.name} on ${on_string}: Assembled Transcripts" from_work_dir="trinity_out_dir/Trinity.fasta"/>
    </outputs>
    <tests>
    </tests>
    <help>
        Trinity is a de novo transcript assembler that uses RNA-seq data as input. This tool runs all Trinity_ commands--Inchworm, Chrysalis, and Butterfly--in a single pass.
        
        .. _Trinity: http://trinityrnaseq.sourceforge.net
    </help>
</tool>