Mercurial > repos > anton > vcfprimers
comparison vcfprimers.xml @ 3:f3243301d74f draft default tip
Uploaded
| author | anton |
|---|---|
| date | Mon, 15 Sep 2014 14:53:14 -0400 |
| parents | 47dd84123b42 |
| children |
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| 2:47dd84123b42 | 3:f3243301d74f |
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| 1 <tool id="vcfprimers" name="VCFprimers:" version="0.0.2"> | 1 <tool id="vcfprimers" name="VCFprimers:" version="0.0.2"> |
| 2 <requirements> | 2 <requirements> |
| 3 <requirement type="package" version="86723982aa">vcflib</requirement> | 3 <requirement type="package" version="8a5602bf07">vcflib</requirement> |
| 4 <!-- <requirement type="package" version="0.1.18">samtools</requirement> --> | 4 <!-- <requirement type="package" version="0.1.18">samtools</requirement> --> |
| 5 </requirements> | 5 </requirements> |
| 6 <description>Extract flanking sequences for each VCF record</description> | 6 <description>Extract flanking sequences for each VCF record</description> |
| 7 <command> | 7 <command> |
| 8 #set $reference_fasta_filename = "localref.fa" | 8 #set $reference_fasta_filename = "localref.fa" |
| 47 <output name="out_file1" file="vcfprimers-test1.fasta"/> | 47 <output name="out_file1" file="vcfprimers-test1.fasta"/> |
| 48 </test> | 48 </test> |
| 49 </tests> | 49 </tests> |
| 50 <help> | 50 <help> |
| 51 | 51 |
| 52 For each VCF record, extract the flanking sequences, and write them to stdout as FASTA | 52 For each VCF record, extract the flanking sequences, and write them as FASTA |
| 53 records suitable for alignment. This tool is intended for use in designing validation | 53 records suitable for alignment. This tool is intended for use in designing validation |
| 54 experiments. Primers extracted which would flank all of the alleles at multi-allelic | 54 experiments. Primers extracted which would flank all of the alleles at multi-allelic |
| 55 sites. The name of the FASTA "reads" indicates the VCF record which they apply to. | 55 sites. The name of the FASTA "reads" indicates the VCF record which they apply to. |
| 56 The form is >CHROM_POS_LEFT for the 3' primer and >CHROM_POS_RIGHT for the 5' primer, | 56 The form is >CHROM_POS_LEFT for the 3' primer and >CHROM_POS_RIGHT for the 5' primer, |
| 57 for example:: | 57 for example:: |
