Mercurial > repos > artbio > gsc_filter_genes
annotate filter_genes.xml @ 1:5d2304b09f58 draft
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit d9e43e514d5fe7bbc7205a52ccb3e4faef50e856
author | artbio |
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date | Mon, 24 Jun 2019 18:07:05 -0400 |
parents | f689c4ea8c43 |
children | afe949d332b3 |
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1
5d2304b09f58
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit d9e43e514d5fe7bbc7205a52ccb3e4faef50e856
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1 <tool id="filter_genes" name="Filter genes in single cell data" version="0.9.1"> |
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f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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2 <description>which are detected in less that a given fraction of the libraries</description> |
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planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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3 <requirements> |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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4 <requirement type="package" version="1.3.2=r3.3.2_0">r-optparse</requirement> |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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5 </requirements> |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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6 <stdio> |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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7 <exit_code range="1:" level="fatal" description="Tool exception" /> |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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8 </stdio> |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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9 <command detect_errors="exit_code"><![CDATA[ |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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10 Rscript $__tool_directory__/filter_genes.R |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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11 --input $input |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
artbio
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12 --sep |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
artbio
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13 #if $sep == 'tab': |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
artbio
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14 'tab' |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
artbio
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15 #elif $sep == 'comma': |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
artbio
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16 'comma' |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
artbio
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17 #end if |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
artbio
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18 --colnames '$colnames' |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
artbio
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19 --percentile_detection '$percentile_detection' |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
artbio
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20 --absolute_detection '$absolute_detection' |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
artbio
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21 --output $output |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
artbio
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22 ]]></command> |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
artbio
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23 <inputs> |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
artbio
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24 <param name="input" type="data" format="txt,tabular" label="Expression data" help="a csv or tsv table file" /> |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
artbio
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25 <param name="sep" type="select" label="Indicate column separator"> |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
artbio
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26 <option value="tab" selected="true">Tabs</option> |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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27 <option value="comma">Comma</option> |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
artbio
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28 </param> |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
artbio
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29 <param name="colnames" type="select" label="Firt row contains column names"> |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
artbio
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30 <option value="TRUE" selected="true">True</option> |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
artbio
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31 <option value="FALSE">False</option> |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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32 </param> |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
artbio
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33 <param name="percentile_detection" value="0.0" type="float" label="remove genes that are expressed in less than this fraction of cells" |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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34 help="Fraction is expressed as a floatting number < 1" /> |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
artbio
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35 <param name="absolute_detection" value="0" type="integer" label="remove genes that are expressed in less than this number of cells" |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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36 help="an absolute number of cells/libraries" /> |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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37 </inputs> |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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38 <outputs> |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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39 <data name="output" format="tabular" label="Cell data filtered from ${on_string}" /> |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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40 </outputs> |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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41 <tests> |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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42 <test> <!-- null case --> |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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43 <param name="input" value="input.tsv" ftype="txt"/> |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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44 <param name="sep" value='tab' /> |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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45 <param name="colnames" value="TRUE"/> |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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46 <output name="output" file="filtered-null.tab" ftype="tabular"/> |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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47 </test> |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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48 <test> |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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49 <param name="input" value="input.csv" ftype="txt"/> |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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50 <param name="sep" value='comma' /> |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
artbio
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51 <param name="colnames" value="TRUE"/> |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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52 <param name="percentile_detection" value="0.05"/> |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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53 <output name="output" file="filtered-0.05.tab" ftype="tabular"/> |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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54 </test> |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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55 <test> |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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56 <param name="input" value="input.csv" ftype="txt"/> |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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57 <param name="sep" value='comma' /> |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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58 <param name="colnames" value="TRUE"/> |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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59 <param name="percentile_detection" value="0.0"/> |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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60 <param name="absolute_detection" value="5"/> |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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61 <output name="output" file="filtered-5.tab" ftype="tabular"/> |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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62 </test> |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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63 </tests> |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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64 <help> |
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planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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65 |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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66 **What it does** |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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67 |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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68 The tools takes a table of *normalized* gene expression values |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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69 (i.e. log2(CPM+1), TPM, RPK, etc...) from single cell RNAseq sequencing libraries (columns) |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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70 and filters out genes (rows) that are detected in less than the specified fraction of libraries, |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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71 or than an absolute number of libraries. |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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72 |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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73 The criteria ("less than this fraction of cells" or "less than this number of cells") left at 0 is not used. |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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74 If none criteria is set, no gene will be filtered out. If both criteria are set (which is logically impossible), |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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75 the criteria "less than this fraction of cells" will be used by default. |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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76 |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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77 A TSV gene expression table for genes that passed the filter is returned. |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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78 |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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79 **Input** |
f689c4ea8c43
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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80 |
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planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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81 A table of comma (csv) or tabulation (tsv) separated values of _normalized_ gene expressions, |
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planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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82 i.e. log2(CPM+1), TPM, RPK, etc... |
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planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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83 Gene names should be in the first column and cell names should be in the first row. |
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planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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84 Note that in a number of a csv files, header of the gene column is omitted, resulting in |
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planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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85 a first row with one item less than in other rows. Although this is not recommended, the tool |
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planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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86 handles this type of table and will return a filtered table with the same structure. |
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planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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87 |
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planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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88 </help> |
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planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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89 <citations> |
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planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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90 <citation type="bibtex"> |
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planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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91 @Manual{, |
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planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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92 title = {R: A Language and Environment for Statistical Computing}, |
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planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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93 author = {{R Core Team}}, |
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planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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94 organization = {R Foundation for Statistical Computing}, |
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planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_filter_genes commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe
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95 address = {Vienna, Austria}, |
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96 year = {2014}, |
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97 url = {http://www.R-project.org/}, |
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98 } |
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99 </citation> |
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100 </citations> |
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101 </tool> |