annotate combine_metaphlan2_humann2.py @ 1:e25efca0a49c draft

"planemo upload for repository https://github.com/asaim/galaxytools/tree/master/tools/combine_metaphlan2_humann2 commit 4a40a73a6eb981310a3c1c54dfa98b5460a843f9"

#!/usr/bin/env python
# -*- coding: utf-8 -*-

import argparse


def extract_clade_abundance(metaphlan2_fp):
    clade_abund = {}
    with open(metaphlan2_fp, 'r') as metaphlan2_f:
        for line in metaphlan2_f.readlines():
            if line.find('g__') == -1:
                continue

            split_line = line[:-1].split('\t')
            taxo = split_line[0]
            abundance = split_line[1]

            genus = taxo[(taxo.find('g__')+3):]
            if genus.find('|') != -1:
                genus = genus[:(genus.find('|'))]
            clade_abund.setdefault(genus, {'abundance': 0, 'species': {}})
            if taxo.find('t__') != -1:
                continue
            elif taxo.find('s__') != -1:
                species = taxo[(taxo.find('s__')+3):]
                clade_abund[genus]['species'].setdefault(
                    species,
                    abundance)
            else:
                clade_abund[genus]['abundance'] = abundance
    return clade_abund


def compute_overall_abundance(humann2_fp):
    overall_abundance = 0
    with open(humann2_fp, 'r') as humann2_f:
        for line in humann2_f.readlines():
            if line.find('|') != -1 or line.startswith('#'):
                continue
            split_line = line[:-1].split('\t')
            overall_abundance += float(split_line[1])
    return overall_abundance


def format_characteristic_name(name):
    formatted_n = name
    formatted_n = formatted_n.replace('/', ' ')
    formatted_n = formatted_n.replace('-', ' ')
    formatted_n = formatted_n.replace("'", '')
    if formatted_n.find('(') != -1 and formatted_n.find(')') != -1:
        open_bracket = formatted_n.find('(')
        close_bracket = formatted_n.find(')')+1
        formatted_n = formatted_n[:open_bracket] + formatted_n[close_bracket:]
    return formatted_n


def combine_metaphlan2_humann2(args):
    clade_abund = extract_clade_abundance(args.metaphlan2_fp)
    overall_abund = compute_overall_abundance(args.humann2_fp)

    with open(args.output_fp, 'w') as output_f:
        s = 'genus\tgenus_abundance\tspecies\tspecies_abundance\t'
        s = '%s\t%s_id\t%s_name\t%s_abundance\n' % (s, args.type, args.type, args.type)
        output_f.write(s)
        with open(args.humann2_fp, 'r') as humann2_f:
            for line in humann2_f.readlines():
                if line.find('|') == -1:
                    continue

                split_line = line[:-1].split('\t')
                abundance = 100*float(split_line[1])/overall_abund
                annotation = split_line[0].split('|')
                charact = annotation[0].split(':')
                charact_id = charact[0]
                char_name = ''
                if len(charact) > 1:
                    char_name = format_characteristic_name(charact[-1])
                taxo = annotation[1].split('.')

                if taxo[0] == 'unclassified':
                    continue
                genus = taxo[0][3:]
                species = taxo[1][3:]

                if genus not in clade_abund:
                    print("no %s found in %s" % (genus, args.metaphlan2_fp))
                    continue
                if species not in clade_abund[genus]['species']:
                    print("no %s found in %s for % s" % (species, args.metaphlan2_fp, genus))
                    continue

                s = "%s\t%s\t" % (genus, clade_abund[genus]['abundance'])
                s += "%s\t%s\t" % (species, clade_abund[genus]['species'][species])
                s += "%s\t%s\t%s\n" % (charact_id, char_name, abundance)
                output_f.write(s)


if __name__ == '__main__':
    parser = argparse.ArgumentParser()
    parser.add_argument('--humann2_fp', required=True)
    parser.add_argument('--metaphlan2_fp', required=True)
    parser.add_argument('--output_fp', required=True)
    parser.add_argument(
        '--type',
        required=True,
        choices=['gene_families', 'pathways'])
    args = parser.parse_args()

    combine_metaphlan2_humann2(args)