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author | bebatut |
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date | Tue, 21 Jun 2016 08:05:01 -0400 |
parents | b344522045f3 |
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<tool id="export2graphlan" name="Export to GraPhlAn" version="0.19"> <description></description> <requirements> <requirement type="package" version="0.16">pandas</requirement> <requirement type="package" version="0.16">scipy</requirement> <requirement type="package" version="1.4">matplotlib</requirement> <requirement type="package" version="0.19">export2graphlan</requirement> </requirements> <stdio> </stdio> <version_command> <![CDATA[ export2graphlan.py -v ]]> </version_command> <command> <![CDATA[ export2graphlan.py -i $lefse_input #if $input_lefse_output.input_lefse_output_test == "true" -o $input_lefse_output.lefse_output #end if -t $tree -a $annotation #if $annotations --annotations $annotations #end if #if $external_annotations --external_annotations $external_annotations #end if #if $background_levels --background_levels $background_levels #end if #if $background_clades --background_clades "$background_clades" #end if #if $background_colors --background_colors "$background_colors" #end if #if $title --title "title" #end if --title_font_size $title_font_size --def_clade_size $def_clade_size --min_clade_size $min_clade_size --max_clade_size $max_clade_size --def_font_size $def_font_size --min_font_size $min_font_size --max_font_size $max_font_size --annotation_legend_font_size $annotation_legend_font_size --abundance_threshold $abundance_threshold #if $most_abundant --most_abundant $most_abundant #end if #if $least_biomarkers --least_biomarkers $least_biomarkers #end if --fname_row $fname_row --sname_row $sname_row #if $metadata_rows --metadata_rows $metadata_rows #end if #if $skip_rows --skip_rows $skip_rows #end if #if $sperc --sperc $sperc #end if #if $fperc --fperc $fperc #end if #if $stop --stop $stop #end if #if $ftop --ftop $ftop #end if ]]> </command> <inputs> <param name="lefse_input" type="data" format="tabular,txt,tsv" label="Input file" help="Identical to LEfSe input file (--lefse_input)"/> <conditional name="input_lefse_output"> <param name="input_lefse_output_test" type="select" label="Use a LEfSe output file as input?" help=""> <option value="true">Yes</option> <option value="false" selected="true">No</option> </param> <when value="true"> <param name="lefse_output" type="data" format="tabular" label="Input file corresponding to LEfSe output file" help="(--lefse_output)"/> </when> <when value="false"/> </conditional> <param name="annotations" type="text" label="List which levels should be annotated in the tree (Optional)" help="The levels must be in comma-separated form (--annotations)" optional="True"/> <param name="external_annotations" type="text" label="List which levels should use the external legend for the annotation (Optional)" help="The levels must be in comma-separated form (--external_annotations)" optional="True"/> <param name="background_levels" type="text" label="List which levels should be highlight with a shaded background (Optional)" help="The levels must be in comma-separated form (--background_levels)" optional="True"/> <param name="background_clades" type="text" label="List of the clades that should be highlight with a shaded background (Optional)" help="The clades must be in comma-separated form (--background_clades)" optional="True"/> <param name="background_colors" type="text" label="List of color to use for the shaded background (Optional)" help="Colors can be either in RGB or HSV (using a semi-colon to separate values, surrounded with ()). The different colors must be in comma-separated form (--background_colors)" optional="True"/> <param name="title" type="text" label="Title of the GraPhlAn plot (Optional)" help="(--title)" optional="True"/> <param name="title_font_size" type="integer" value="15" label="Title font size" help="(--title_font_size)"/> <param name="def_clade_size" type="integer" value="10" label="Default size for clades that are not found as biomarkers" help="(--def_clade_size)"/> <param name="min_clade_size" type="integer" value="20" label="Minimum value of clades that are biomarkers" help="(--min_clade_size)"/> <param name="max_clade_size" type="integer" value="200" label="Maximum value of clades that are biomarkers" help="(--max_clade_size)"/> <param name="def_font_size" type="integer" value="10" label="Default font size" help="(--def_font_size)"/> <param name="min_font_size" type="integer" value="8" label="Minimum font size" help="(--min_font_size)"/> <param name="max_font_size" type="integer" value="12" label="Maximum font size" help="(--max_font_size)"/> <param name="annotation_legend_font_size" type="integer" value="10" label="Font size for the annotation legend" help="(--annotation_legend_font_size)"/> <param name="abundance_threshold" type="float" value="20.0" label="Minimun abundance value for a clade to be annotated" help="(--abundance_threshold)"/> <param name="most_abundant" type="integer" label="Number of clades to highlight (Optional)" help="(--most_abundant)" optional="True"/> <param name="least_biomarkers" type="integer" label="Minimum number of biomarkers to extract (Optional)" help=" The taxonomy is parsed, and the level is choosen in order to have at least the specified number of biomarkers(--least_biomarkers)" optional="True"/> <param name="fname_row" type="integer" value="0" label="Row number containing the names of the features" help="-1 specifies that no names are present in the matrix (--fname_row)"/> <param name="sname_row" type="integer" value="0" label="Row number containing the names of the samples" help="-1 specifies that no names are present in the matrix (--sname_row)"/> <param name="metadata_rows" type="integer" label="Row number to use as metadata (Optional)" help="(--metadata_rows)" optional="True"/> <param name="skip_rows" type="text" label="Row number to skip from the input file" help="The row numbers ar indexed to 0 and comma separated (--skip_rows)" optional="True"/> <param name="sperc" type="float" label="Percentile of sample value distribution for sample selection (Optional)" help="(--sperc)" optional="True"/> <param name="fperc" type="float" label="Percentile of feature value distribution for sample selection (Optional)" help="(--fperc)" optional="True"/> <param name="stop" type="integer" label="Number of top samples to select (Optional)" help="The order is based on percentile specified by --sperc (--stop)" optional="True"/> <param name="ftop" type="integer" label="Number of top features to select (Optional)" help="The order is based on percentile specified by --fperc (--ftop)" optional="True"/> </inputs> <outputs> <data format="txt" name="tree" label="${tool.name} on ${on_string}: Tree" /> <data format="txt" name="annotation" label="${tool.name} on ${on_string}: Annotation" /> </outputs> <tests> <test> <param name="lefse_input" value="input_profile.tabular"/> <param name="input_lefse_output_test" value="no" /> <param name="annotations" value=""/> <param name="external_annotations" value=""/> <param name="background_levels" value=""/> <param name="background_clades" value=""/> <param name="background_colors" value=""/> <param name="title" value=""/> <param name="title_font_size" value="15"/> <param name="def_clade_size" value="10"/> <param name="min_clade_size" value="20"/> <param name="max_clade_size" value="200"/> <param name="def_font_size" value="10"/> <param name="min_font_size" value="8"/> <param name="max_font_size" value="12"/> <param name="annotation_legend_font_size" value="10"/> <param name="abundance_threshold" value="20.0"/> <param name="most_abundant" value=""/> <param name="least_biomarkers" value=""/> <param name="fname_row" value="0"/> <param name="sname_row" value="0" /> <param name="metadata_rows" value=""/> <param name="skip_rows" value=""/> <param name="sperc" value=""/> <param name="fperc" value=""/> <param name="stop" value=""/> <param name="ftop" value=""/> <output name="annotation" file="annotation_output.txt"/> <output name="tree" file="tree_output.txt"/> </test> </tests> <help><![CDATA[ **What it does** export2graphlan is a conversion software tool to produce both annotation and tree file for GraPhlAn. It can convert MetaPhlAn, LEfSe, and/or HUMAnN output to GraPhlAn input format In particular, the annotation file tries to highlight specific sub-trees deriving automatically from input file what nodes are important. For more information, check the `user manual <https://bitbucket.org/CibioCM/export2graphlan/overview/>`_. ]]></help> <citations> </citations> </tool>