annotate glimmer_build_icm.xml @ 0:9c195b26a5ac draft

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date Tue, 28 Nov 2017 10:08:06 -0500
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1 <tool id="glimmer_build_icm" name="Glimmer ICM builder" version="@WRAPPER_VERSION@">
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2 <description></description>
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3 <macros>
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4 <import>macros.xml</import>
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5 </macros>
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6 <expand macro="requirements"/>
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7 <command><![CDATA[
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8 build-icm
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9 --depth $depth
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10 $no_stops
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11 --period $period
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12 --width $width
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13
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14 #if $stop_codon_opts.stop_codon_opts_selector == "gb":
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15 --trans_table '${stop_codon_opts.genbank_gencode}'
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16 #else:
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17 --stop_codons '${stop_codon_opts.stop_codons}'
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18 #end if
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19
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20 '$outfile' < '$infile' 2>&1;
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21 ]]></command>
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22 <inputs>
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23 <param name="infile" type="data" format="fasta" label="Trainings Dataset" help="A set of known genes in FASTA format." />
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24 <param name="depth" type="integer" value="7" label="Set the depth of the ICM" help="The depth is the maximum number of positions in the context window that will be used to determine the probability of the predicted position." />
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25 <param name="period" type="integer" value="3" label="Set the period of the ICM" help="The period is the number of different submodels for different positions in the text in a cyclic pattern. E.g., if the period is 3, the first submodel will determine positions 1, 4, 7, ..." />
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26 <param name="width" type="integer" value="12" label="Set the width of the ICM" help="The width includes the predicted position." />
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27 <param name="no_stops" type="boolean" truevalue="--no_stops" falsevalue="" checked="false" label="Do not use any input strings with in-frame stop codons" />
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28
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29 <conditional name="stop_codon_opts">
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30 <param name="stop_codon_opts_selector" type="select" label="Specify start codons as">
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31 <option value="gb" selected="True">Genbank translation table entry</option>
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32 <option value="free_form">Comma-separated list</option>
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33 </param>
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34 <when value="gb">
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35 <param name="genbank_gencode" type="select" label="Use Genbank translation table to specify stop codons">
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36 <option value="1" selected="True">1. Standard</option>
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37 <option value="2">2. Vertebrate Mitochondrial</option>
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38 <option value="3">3. Yeast Mitochondrial</option>
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39 <option value="4">4. Mold, Protozoan, and Coelenterate Mitochondrial Code and the Mycoplasma/Spiroplasma Code</option>
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40 <option value="5">5. Invertebrate Mitochondrial</option>
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41 <option value="6">6. Ciliate, Dasycladacean and Hexamita Nuclear Code</option>
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42 <option value="9">9. Echinoderm Mitochondrial</option>
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43 <option value="10">10. Euplotid Nuclear</option>
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44 <option value="11">11. Bacteria and Archaea</option>
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45 <option value="12">12. Alternative Yeast Nuclear</option>
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46 <option value="13">13. Ascidian Mitochondrial</option>
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47 <option value="14">14. Flatworm Mitochondrial</option>
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48 <option value="15">15. Blepharisma Macronuclear</option>
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49 <option value="16">16. Chlorophycean Mitochondrial</option>
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50 <option value="21">21. Trematode Mitochondrial</option>
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51 <option value="22">22. Scenedesmus obliquus mitochondrial</option>
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52 <option value="23">23. Thraustochytrium Mitochondrial</option>
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53 <option value="24">24. Pterobranchia mitochondrial</option>
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54 </param>
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55 </when>
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56 <when value="free_form">
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57 <param name="stop_codons" type="text" value="tag,tga,taa" label="Specify stop codons as a comma-separated list" />
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58 </when>
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59 </conditional>
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60 </inputs>
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61 <outputs>
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62 <data format="data" name="outfile" />
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63 </outputs>
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64 <tests>
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65 <test>
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66 <param name="infile" value='streptomyces_Tu6071_plasmid_genes.fasta' />
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67 <param name="depth" value="7" />
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68 <param name="period" value="3" />
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69 <param name="width" value="12" />
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70 <param name="no_stops" value="" />
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71 <param name="genbank_gencode" value="11" />
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72 <!-- compare files sizes, because the output is a binary -->
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73 <output name="outfile" file='streptomyces_Tu6071_plasmid_genes.icm' compare="sim_size" delta="1000" ftype="data" />
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74 </test>
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75 </tests>
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76
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77 <help>
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78 <![CDATA[
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79
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80 **What it does**
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81
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82 This program constructs an interpolated context model (ICM) from an input set of sequences.
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83
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84 This model can be used by Glimmer3 to predict genes.
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85
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86 **TIP** To extract CDS from a GenBank file use the tool *Extract ORF from a GenBank file*.
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87
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88 -----
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89
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90 **Example**
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91
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92 *Input*::
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93
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94 - Genome Sequence
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95
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96 >CELF22B7 C.aenorhabditis elegans (Bristol N2) cosmid F22B7
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97 GATCCTTGTAGATTTTGAATTTGAAGTTTTTTCTCATTCCAAAACTCTGT
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98 GATCTGAAATAAAATGTCTCAAAAAAATAGAAGAAAACATTGCTTTATAT
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99 TTATCAGTTATGGTTTTCAAAATTTTCTGACATACCGTTTTGCTTCTTTT
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100 TTTCTCATCTTCTTCAAATATCAATTGTGATAATCTGACTCCTAACAATC
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101 GAATTTCTTTTCCTTTTTCTTTTTCCAACAACTCCAGTGAGAACTTTTGA
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102 ATATCTTCAAGTGACTTCACCACATCAGAAGGTGTCAACGATCTTGTGAG
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103 AACATCGAATGAAGATAATTTTAATTTTAGAGTTACAGTTTTTCCTCCGA
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104 CAATTCCTGATTTACGAACATCTTCTTCAAGCATTCTACAGATTTCTTGA
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105 TGCTCTTCTAGGAGGATGTTGAAATCCGAAGTTGGAGAAAAAGTTCTCTC
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106 AACTGAAATGCTTTTTCTTCGTGGATCCGATTCAGATGGACGACCTGGCA
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107 GTCCGAGAGCCGTTCGAAGGAAAGATTCTTGTGAGAGAGGCGTGAAACAC
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108 AAAGGGTATAGGTTCTTCTTCAGATTCATATCACCAACAGTTTGAATATC
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109 CATTGCTTTCAGTTGAGCTTCGCATACACGACCAATTCCTCCAACCTAAA
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110 AAATTATCTAGGTAAAACTAGAAGGTTATGCTTTAATAGTCTCACCTTAC
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111 GAATCGGTAAATCCTTCAAAAACTCCATAATCGCGTTTTTATCATTTTCT
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112 .....
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113
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114 *Output*::
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115 interpolated context model (ICM)
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116
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117
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118
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119 ]]>
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120 </help>
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121 <expand macro="citation" />
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122 </tool>