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author | iuc |
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date | Mon, 16 Dec 2024 14:42:47 +0000 |
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<tool id="glimmer_gbk_to_orf" name="Extract ORF" version="@WRAPPER_VERSION@" profile="@PROFILE_VERSION@"> <description>from a GenBank file</description> <macros> <import>macros.xml</import> </macros> <expand macro="bio_tools"/> <expand macro="requirements"/> <command><![CDATA[ python '$__tool_directory__/glimmer_gbk_to_orf.py' -g '$infile' -a '$aa_output' -n '$nc_output' ##TODO translation table, can be extracted from genbank file directly ]]></command> <inputs> <param name="infile" type='data' format="genbank" label="gene bank file"/> </inputs> <outputs> <data name="aa_output" format="fasta" label="${tool.name} on ${on_string}: Amino acids"/> <data name="nc_output" format="fasta" label="{tool.name} on ${on_string}: Nucleotides"/> </outputs> <tests> <test> <param name="infile" value="test.gbk" /> <output name="aa_output" file="orf_aa.fa" /> <output name="nc_output" file="orf_nc.fa" /> </test> </tests> <help><![CDATA[ **What it does** Read a GenBank file and export fasta formatted amino acid and CDS files. ----- **Example** * input:: Genebankfile LOCUS BA000030 9025608 bp DNA linear BCT 21-DEC-2007 DEFINITION Streptomyces avermitilis MA-4680 DNA, complete genome. ACCESSION BA000030 AP005021-AP005050 VERSION BA000030.3 GI:148878541 DBLINK Project: 189 KEYWORDS . SOURCE Streptomyces avermitilis MA-4680 ORGANISM Streptomyces avermitilis MA-4680 Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales; Streptomycineae; Streptomycetaceae; Streptomyces. REFERENCE 1 AUTHORS Omura,S., Ikeda,H., Ishikawa,J., Hanamoto,A., Takahashi,C., Shinose,M., Takahashi,Y., Horikawa,H., Nakazawa,H., Osonoe,T., Kikuchi,H., Shiba,T., Sakaki,Y. and Hattori,M. TITLE Genome sequence of an industrial microorganism Streptomyces avermitilis: deducing the ability of producing secondary metabolites JOURNAL Proc. Natl. Acad. Sci. U.S.A. 98 (21), 12215-12220 (2001) PUBMED 11572948 REFERENCE 2 AUTHORS Ikeda,H., Ishikawa,J., Hanamoto,A., Shinose,M., Kikuchi,H., Shiba,T., Sakaki,Y., Hattori,M. and Omura,S. TITLE Complete genome sequence and comparative analysis of the industrial microorganism Streptomyces avermitilis JOURNAL Nat. Biotechnol. 21 (5), 526-531 (2003) PUBMED 12692562 REFERENCE 3 (bases 1 to 9025608) AUTHORS Omura,S., Ikeda,H., Ishikawa,J., Hanamoto,A., Takahashi,C., Shinose,M., Takahashi,Y., Horikawa,H., Nakazawa,H., Osonoe,T., Kushida,N., Shiba,T., Sakaki,Y. and Hattori,M. TITLE Direct Submission JOURNAL Submitted (29-MAR-2002) Contact:S Omura Kitasato University, Kitasato Institute for Life Sciences; 1-15-1 Kitasato, Sagamihara, Kanagawa 228-8555, Japan URL :http://avermitilis.ls.kitasato-u.ac.jp/ COMMENT On Jun 15, 2007 this sequence version replaced gi:57546753. This work was done in collaboration with Haruo Ikeda(*1), Jun Ishikawa(*2), Akiharu Hanamoto(*3), Chigusa Takahashi(*3), Mayumi Shinose(*3), Hiroshi Horikawa(*4), Hidekazu Nakazawa(*4), Tomomi Osonoe(*4), Norihiro Kushida(*4), Hisashi Kikuchi(*4), Tadayoshi Shiba(*5), Yoshiyuki Sakaki(*6,*7), Masahira Hattori(*1,*7) and Satoshi Omura(*1,*3). Final finishing process and all annotation were done by H. Ikeda and J. Ishikawa. *1 Kitasato Institute for Life Sciences, Kitasato University *2 National Institute of Infectious Diseases *3 The Kitasato Institute *4 National Institute of Technology and Evaluation *5 School of Science, Kitasato University *6 Institute of Medical Science, University of Tokyo *7 RIKEN, Genomic Sciences Center All the annotated genes identified are available from following urls. http://avermitilis.ls.kitasato-u.ac.jp. FEATURES Location/Qualifiers source 1..9025608 /organism="Streptomyces avermitilis MA-4680" /mol_type="genomic DNA" /strain="MA-4680" /db_xref="taxon:227882" /note="This strain is also named as strain: ATCC 31267, NCIMB 12804 or NRRL 8165." gene complement(1380..1811) /locus_tag="SAV_1" CDS complement(1380..1811) /locus_tag="SAV_1" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="BAC67710.1" /db_xref="GI:29603637" /translation="MTAEWYVLVEEDTRETKRADGVELRLHRWKLAATQHIAGDQEQA AAAAEDAALNYMPGVLARHARPGDEPARHAFLTQDGAWLVLLRQRHRECHIRVTTARL MHTQEEKEAPPKSFKEKLRSALDGPQPPEPAGRPWKPGSET" * output:: - aminoAcidOutput >SAV_1 MTAEWYVLVEEDTRETKRADGVELRLHRWKLAATQHIAGDQEQAAAAAEDAALNYMPGVL ARHARPGDEPARHAFLTQDGAWLVLLRQRHRECHIRVTTARLMHTQEEKEAPPKSFKEKL RSALDGPQPPEPAGRPWKPGSET >SAV_2 VPPQGARGTIVSATGSGKTSMAAASTLNCFPEGRILVTVPTLDLLAQTAQAWRAVGHHSP MIAVCSLENDPVLNERT >SAV_3 MDWNFPDDDIFFCGGCGDDDTPDPRVPRQDKALCVRCDRVERQVRRYRITVPRRNAIMRF QRDVCALCQEGPPTDHCPDAVSFWHIDHDHRCCPPGGSCGRCVRGLLCLPCNATRLPAYE RLPNVLRDSPRFNTYLNSPPARHPEARPTARDHAGPRDASSYLIDAFFTAADHPEGNALS S >SAV_4 VALTPGGTRVTQWQDRQAIGDMHERRVAAALRARGWTVQPCGQGTYPPAVREALRRTRSA LRHFPDLIAARGADLITIDAKDRMPSTDTDRYAVSADTVTAGLFFTAAHAPTPLYYVFGD LKVLTPAEVVHYTAHALRHRSGAFHLVRTEQAHCFDDVFGSAGAAAAA >SAV_5 MMLLMAAYVDPRFRPTLWPGTPVPTPELMPLRGARADGEWIVWTPQVRSRSHTVPVPEDF YLREFMEVDPEDLDAVAALMGAYGHLGGSINTGSWDVDVYERLKELTEREHPRAPFALHG ELATLFMREAQAAITTWLALRREGGLDALIEPEVSEEELAQWQASNADLEEAWPRDLDHL RELSLEIRISNLVSELNAALKPFSIGIGGLGDRYPTILAVAFLQLYNHLAEDATIRECAN ETCRRHFVRQRGRAAYGQNRTSGIKYCTRECARAQAQREHRRRRKQQTTTLQQPPAPGPQ SHDTSEPTAEGR >SAV_6 MISLREHQVEANARIRAWAGFPTRSPVPAQGLRGTVVSATGSGKTITAAWAARECFRGGR ILVMVPTLDLLVQTAQAWRRVGHNGPMVAACSLEKDEVLEQLGVRTTTNPIQLALWAGHG PVVVFATYASLVDREDPEDVTGRAKVRGPLEAALAGGQRLYGQTMDGFDLAVVDEAHSTT GDLGRPWAAIHDNSRIPADFRLYLTATPRILASPRPQKGADGRELEIATMASDPDGPYGE WLFELGLSEAVERGILAGFEIDVLEIRDPSPALGESEEAQRGRRLALLQTALLEHAAARN LRTVMTFHQRVEEAAAFAQTMPQTAARLYEAEVSAEALVDAGALPESSIGAEFYELEAGR HVPPDRVWAAWLCGDHLVAERREVLRQFADGLDAGNKRVHRAFLASVRVLGEGVDIVGER GVEAICFADTRGSQVEIVQNIGRALRPNPDGTNKTARIIVPVFLQPGENPTDMVASASFA PLVTVLQGLRSHSERLVEQLASRALTSGQRHVHVKRDEDGRIIGTTTEGEGGQHESEGAV ESALLHFSTPRDATTIAAFLRTRVYRPESLVWLEGYQALLRWRKKNHITGLYAVPYDTET EAGVTKAFPLGRWVHQQRRTYRAGELDPHRTTLLDEAGMVWEPGDEAWENKLAALRSFHR AHGHLAPRRDAVWGDADSELVPVGEHMANLRRKDGLGKNPQRAATRATQLAAIDPDWNCP WPLDWQRHYRVLADLATDEPHSRLPDIQPGVQFEGDDLGKWLQRQRRSWAELSEEQQQRL TALGVTPAEPPTPTPSAKGGGKAAAFQRGLAALAQWIQREGAHKVVPRGHVEAVVIDGQE HQHKLGVWISNTKTRRDKLTHDQRTALAALGVEWA .... - orfs >SAV_1 ATGACCGCCGAGTGGTACGTCCTCGTCGAAGAGGACACACGAGAGACCAAGCGCGCCGAC GGCGTTGAACTCAGATTGCACCGCTGGAAACTGGCGGCCACTCAGCACATCGCAGGAGAT CAGGAACAGGCCGCCGCCGCGGCCGAGGATGCGGCCCTGAACTACATGCCGGGAGTGCTC GCTCGGCATGCCCGACCGGGAGACGAACCGGCCCGGCATGCTTTCCTCACCCAGGACGGG GCCTGGCTGGTGCTCCTCAGGCAGCGGCACCGCGAGTGTCACATACGGGTGACCACTGCC CGGCTCATGCATACACAGGAAGAGAAGGAGGCCCCGCCGAAAAGCTTCAAGGAGAAACTC CGCAGCGCCCTGGATGGTCCTCAGCCGCCCGAACCGGCTGGTAGGCCATGGAAGCCGGGC AGCGAAACCTGA >SAV_2 GTGCCCCCTCAGGGAGCCCGTGGCACGATCGTGTCAGCTACCGGGTCCGGCAAAACGAGC ATGGCCGCCGCGAGCACGCTGAACTGCTTCCCCGAAGGCCGGATCCTCGTGACCGTGCCG ACCCTGGACCTGCTCGCACAGACCGCCCAGGCGTGGCGGGCAGTCGGCCACCACTCCCCC ATGATCGCGGTGTGCTCGCTGGAGAACGACCCAGTGCTGAACGAGCGGACCTGA >SAV_3 ATGGACTGGAACTTCCCCGACGACGACATCTTCTTCTGCGGCGGGTGCGGCGACGACGAC ACCCCCGACCCGCGGGTCCCGCGTCAGGACAAGGCCCTGTGCGTCCGCTGCGACAGAGTC GAACGGCAGGTCCGCCGATACCGGATCACCGTGCCGCGGAGGAACGCGATCATGCGCTTC CAGCGCGACGTCTGCGCCCTGTGCCAGGAAGGCCCGCCGACCGACCACTGCCCCGATGCC GTCAGCTTCTGGCACATCGACCACGACCACCGCTGCTGCCCTCCCGGCGGCTCATGCGGG CGGTGCGTCCGCGGCCTCCTGTGCCTGCCCTGCAACGCCACCCGCCTGCCCGCCTACGAA CGCCTCCCCAACGTCCTCCGCGACAGCCCTCGCTTCAACACCTACCTCAACAGCCCACCC GCCCGGCACCCCGAAGCCCGCCCCACCGCCAGGGACCATGCAGGCCCCCGCGACGCATCC AGCTACCTCATCGACGCCTTTTTCACCGCCGCGGACCATCCCGAGGGGAACGCCCTCAGC TCCTGA >SAV_4 GTGGCACTTACCCCAGGGGGAACCCGAGTGACGCAGTGGCAGGACCGCCAGGCGATAGGC GACATGCACGAACGTCGGGTGGCGGCCGCGCTGCGCGCCCGCGGCTGGACCGTCCAGCCC TGCGGACAGGGCACCTACCCGCCCGCCGTACGGGAAGCCCTGCGCCGGACCCGCTCCGCC CTGCGGCACTTCCCCGACCTCATCGCCGCCCGCGGCGCCGACCTGATCACCATCGACGCC AAGGACCGCATGCCCAGCACCGACACCGACCGCTACGCCGTCAGCGCCGACACCGTGACC GCCGGCCTCTTTTTCACCGCGGCCCACGCTCCGACTCCGCTGTACTACGTCTTCGGCGAC CTGAAGGTCCTCACGCCGGCGGAGGTGGTCCACTACACCGCTCACGCCTTGCGCCACCGC AGCGGTGCCTTCCACCTCGTACGCACGGAGCAAGCACACTGCTTCGACGACGTCTTCGGA TCGGCTGGCGCAGCAGCTGCGGCATGA >SAV_5 ATGATGCTCCTCATGGCGGCATACGTTGACCCACGCTTTCGTCCTACGCTATGGCCTGGA ACGCCCGTGCCGACACCGGAGTTGATGCCTCTTCGCGGAGCGCGGGCCGACGGTGAATGG ATCGTCTGGACCCCGCAGGTCCGCTCCCGCTCGCACACGGTCCCCGTGCCGGAGGACTTC TACCTGCGCGAGTTCATGGAGGTCGACCCTGAGGACCTCGACGCCGTGGCCGCCCTGATG GGCGCCTACGGACACCTCGGCGGGAGCATCAACACCGGAAGCTGGGACGTCGACGTCTAC GAGCGCCTCAAGGAGCTCACGGAGCGCGAACACCCCCGCGCGCCGTTCGCCCTGCACGGC GAACTGGCCACGCTGTTCATGAGGGAGGCGCAGGCGGCCATCACCACCTGGCTGGCCCTG CGCCGCGAGGGCGGGCTCGACGCGCTCATCGAGCCCGAGGTGTCCGAGGAAGAACTGGCG CAGTGGCAAGCGAGCAACGCTGATCTTGAGGAAGCGTGGCCGCGGGACCTGGACCACCTG CGCGAACTCTCCCTGGAGATCAGGATCAGCAACCTCGTGAGCGAACTGAACGCCGCGCTG AAGCCGTTCAGCATCGGCATCGGCGGCCTGGGCGACCGCTACCCCACCATCCTCGCTGTG GCGTTCCTCCAGCTCTACAACCACCTCGCCGAGGACGCCACGATCCGCGAGTGCGCGAAC GAGACCTGCCGCCGCCACTTCGTACGCCAGCGCGGCCGCGCCGCATACGGGCAGAACCGC ACCAGCGGCATCAAGTACTGCACCCGCGAATGCGCCCGCGCCCAGGCCCAGCGCGAACAC CGCCGGCGCCGCAAACAGCAGACCACGACCCTCCAGCAGCCGCCGGCGCCTGGTCCTCAG TCTCACGACACCTCAGAGCCGACTGCCGAAGGGCGCTGA ....... ]]></help> <expand macro="citation" /> </tool>