view hicCompartmentalization.xml @ 6:ed3dea19c464 draft default tip

"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hicexplorer commit 2a0943e78bdc8ebb13f181399206a9eea37ed78f"
author iuc
date Tue, 16 Mar 2021 14:56:24 +0000
parents c6f32406aa3c
children
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<tool id="hicexplorer_hiccompartmentspolarization" name="@BINARY@" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@">
    <description>compute pairwise correlations between multiple Hi-C contact matrices</description>
    <macros>
        <token name="@BINARY@">hicCompartmentalization</token>
        <import>macros.xml</import>
    </macros>
    <expand macro="requirements" />
    <command detect_errors="exit_code"><![CDATA[

        #for $counter, $m in enumerate($matrix_h5_cooler_multiple):
            ln -s '$m' '${counter}_matrix.$m.ext' &&
        #end for
        #set $m = ' '.join([ '\'%s_matrix.%s\'' % ($counter, $matrix.ext) for $counter, $matrix in enumerate($matrix_h5_cooler_multiple) ])
       
        @BINARY@
            --obsexp_matrices $m
            --pca '$pca'
            --quantile $quantile

            #if $set_offset_value:
                #set $offset_array = $set_offset_value.split(' ')
                #set $offset = ' '.join([ '%s' % int($value) for $value in $offset_array ])
                --offset $offset
            #end if
            --outliers $outliers
            --outputFileName plot.$image_file_format
        && mv plot.$image_file_format plot
]]>
    </command>
    <inputs>
        <expand macro="matrix_h5_cooler_multiple_macro" />
        <param name='pca' type="data" format="bedgraph" label="Eigenvector file" help='A PCA vector as a bedgraph file with no header. In case of several matrices with 
                    different conditions, ie. control treatment, the PCA of control can be 
                    used. Note that only one PCA can be provided.' />
        <param name='quantile' type="integer" value="30" label="Quantile" help='The number of to be used quantiles.' />
        <param name='outliers' type="float" value="0.0" label="Outliers" help='The number of outliers to be removed.' />
        <param name="set_offset_value" type="text" optional='True' value="" label="Offset values">
            <validator type="expression" message="Only numeric characters and the a space as seperator are allowed.">value.replace(' ', '').isnumeric()</validator>
        </param>

        <param name="image_file_format" type="select" label="Image output format">
            <option value="png" selected="True">png</option>
            <option value="svg">svg</option>
            <option value="pdf">pdf</option>
        </param>
    </inputs>
    <outputs>
        <data name="outFileName" format="png" from_work_dir="plot" label="${tool.name} [${on_string}]: Plot">
            <change_format>
                <when input="image_file_format" value="svg" format="svg" />
                <when input="image_file_format" value="pdf" format="pdf" />
            </change_format>
        </data>
        <data name='output_matrix' from_work_dir='output_matrix.npz' format='binary' label='Output .npz file includes all the generated matrices'>
            <filter>outputMatrix == '--outputMatrix output_matrix.npz'</filter>
        </data>
    </outputs>
    <tests>
        <test>
            <param name="matrix_h5_cooler_multiple" value="hicTransform/obs_exp_norm.h5" />
            <param name="pca" value="hicCompartmentsPolarization/pca1.bedgraph" />
            <output name="outFileName" file="hicCompartmentsPolarization/compartmentsPolarizationRatio.png" ftype="png" compare="sim_size" />
        </test>
        <test>
            <param name="matrix_h5_cooler_multiple" value="hicTransform/obs_exp_norm.h5" />
            <param name="pca" value="hicCompartmentsPolarization/pca1.bedgraph" />
            <param name="quantile" value='30' />
            <output name="outFileName" file="hicCompartmentsPolarization/compartmentsPolarizationRatio.png" ftype="png" compare="sim_size" />
        </test>
        <test>
            <param name="matrix_h5_cooler_multiple" value="hicTransform/obs_exp_norm.h5" />
            <param name="pca" value="hicCompartmentsPolarization/pca1.bedgraph" />
            <param name="outliers" value='1.0' />
            <output name="outFileName" file="hicCompartmentsPolarization/compartmentsPolarizationRatio.png" ftype="png" compare="sim_size" />
        </test>
    </tests>
    <help><![CDATA[

Comparmentalization
===================

**hicCompartmentalization** rearranges the average interaction frequencies using the first PC values to represent the global compartmentalization signal.
To our knowledge this has been first introduced and implemented by Wibke Schwarzer et al. 2017 (Nature. 2017 Nov 2; 551(7678): 51–56)


For more information about HiCExplorer please consider our documentation on readthedocs.io_.

.. _readthedocs.io: http://hicexplorer.readthedocs.io/en/latest/index.html

]]>    </help>
    <expand macro="citations" />
</tool>