Mercurial > repos > brenninc > tagdust_2_31

changeset 0:40c86e14b674 draft

Find changesets by keywords (author, files, the commit message), revision number or hash, or revset expression.
Uploaded first version of tagdust
author brenninc
date Mon, 09 May 2016 04:08:01 -0400
parents
children da90c37031bb
files architecture.xml macros.xml paired.xml single.xml test-data/paired_READ1.fq test-data/paired_READ2.fq test-data/paired_un_READ1.fq test-data/paired_un_READ2.fq test-data/read1.fastq test-data/read1.fq test-data/read1_extracted.fastq test-data/read1_un.fq test-data/read2_extracted.fastq test-data/with_b_.fq test-data/with_b_BC_AAC.fq test-data/with_b_BC_GTA.fq test-data/with_b_un.fq tool-data/all_fasta.loc.sample tool-data/tagdust_architecture.loc.sample tool_data_table_conf.xml.sample tool_dependencies.xml
diffstat 20 files changed, 2964 insertions(+), 0 deletions(-) [+]
line wrap: on
line diff
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/architecture.xml	Mon May 09 04:08:01 2016 -0400
@@ -0,0 +1,42 @@
+<tool id="tagdust_architecture" name="Tagdust Architecture viewer" version="0.2">
+    <description>Displays a tagdust Architecture file</description>
+    <macros>
+        <import>macros.xml</import>
+    </macros>
+    <expand macro="requirements"></expand>
+    <expand macro="stdio"></expand>
+    <expand macro="version_command"></expand>
+    <command >
+        cp $architecture.fields.path architecture.txt ;
+    </command>
+    <inputs>
+        <expand macro="architecture"></expand>
+    </inputs>
+    <outputs>
+        <data format="txt" name="architecturefile" label="The tagdust architecture." from_work_dir="architecture.txt"/>
+    </outputs>
+    <tests>
+        <test>
+            <param name="architecture" value="test_F:NNNNNNNN_S:TATAGGG_R:N" /> 
+            <output name="architecturefile" file="../tool-data/F:NNNNNNNN_S:TATAGGG_R:N.txt"/>
+        </test>
+        <test>
+            <param name="architecture" value="test_B:GTA_AAC_R:N" /> 
+            <output name="architecturefile" file="../tool-data/B:GTA,AAC_R:N.txt"/>
+        </test>
+       <test>
+            <param name="architecture" value="test_R:N" /> 
+            <output name="architecturefile" file="../tool-data/R:N.txt"/>
+        </test>
+    </tests>
+     <help>
+<![CDATA[
+This tools simply prints out the selected architecture without running tagdust.
+
+Note both the single end and paired end tagdust tools will report the architecture to standard out. 
+
+]]>    
+@tagdust-documentation@
+     </help>
+     <expand macro="citations"></expand>
+</tool>
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/macros.xml	Mon May 09 04:08:01 2016 -0400
@@ -0,0 +1,179 @@
+<?xml version="1.0"?>
+<macros>
+    <xml name="requirements">
+        <requirements>
+            <requirement type="package" version="2.31">tagdust</requirement>
+        </requirements>
+    </xml>
+    <xml name="version_command">
+        <version_command>tagdust --version | head -n 1 | awk '{ print $2 }'</version_command>
+    </xml>
+    <xml name="stdio">
+        <stdio>
+            <exit_code range="1:" level="fatal" description="Error" />
+        </stdio>
+    </xml>
+    <token name="@cat-archiecture@">
+        echo using architecture ;
+        cat $architecture.fields.path ;
+        echo ;
+    </token>
+    <token name="@tagdust-call-minus-files@">
+        mkdir output ;
+        tagdust -t\${GALAXY_SLOTS:-4} -o output/data 
+            -arch $architecture.fields.path
+            #if $reference_source.reference_source_selector=='history':
+                -ref $reference_source.ref_file
+            #end if
+            #if $reference_source.reference_source_selector=='cached':
+                -ref $reference_source.ref_path.fields.path
+            #end if
+    </token>
+    <token name="@ls_and_barcode@">
+        echo files in output folder ;
+        ls -al output ;
+        #if $include.barcode_files=="yes"
+            #if $architecture.fields.barcode=="yes"
+                cd output ;
+                ls *_BC_* > $barcode_files
+            #else
+                echo Selected architecture not expected to generate any barcode files > $barcode_files ;
+                echo To avoid this output set Choose to find the barcode files to No >> $barcode_files ;
+            #end if
+        #else 
+            #if $architecture.fields.barcode=="yes"
+                echo ;
+                echo Tagdust not included in output at users request! ;
+                echo To get then set Choose to find the barcode files to Yes and run the tool again ;
+            #end if
+        #end if
+    </token>
+    <xml name="architecture">
+         <param name="architecture" type="select" label="Using architecture">
+            <options from_data_table="tagdust_architecture"/>
+            <validator type="no_options" message="No architecture found"/>
+            <!--filter name="barcode" value="yes" column="3"/-->
+        </param>
+    </xml>
+    <xml name="reference_source">
+        <conditional name="reference_source">
+            <param name="reference_source_selector" type="select" label="Choose the source for the reference fasta (If any)">
+                <option value="none"  selected="true">Do not include a reference fasta</option>
+                <option value="cached">Locally cached</option>
+                <option value="history">History</option>
+            </param>
+            <when value="none"/>
+            <when value="cached">
+                <param name="ref_path" type="select" label="Using reference fasta">
+                <options from_data_table="all_fasta"/>
+                    <validator type="no_options" message="A built-in reference fasta is not available for the build associated with the selected input file"/>
+                </param>
+            </when>
+            <when value="history"> 
+                <param name="ref_file" type="data" format="fasta" label="Using reference file" />
+            </when>
+        </conditional>
+    </xml>
+    <xml name="include_barcode_file">
+        <conditional name="include">
+            <param name="barcode_files" type="select" label="Choose to find the barcode files (If any)">
+                <option value="yes" selected="true">Yes. Look for possible barcode separated files.</option>
+                <option value="no" >No. Ignores all barcode files even if these are generated.</option>
+            </param>
+            <when value="yes"/>
+            <when value="no"/>
+        </conditional>
+    </xml>
+    <xml name="output_barcode">
+        <data format="txt" name="barcode_files" label="Tagdust barcoded reads.">
+            <discover_datasets pattern="data_BC_(?P&lt;designation&gt;.+)\.fq" ext="fastq" directory="output" visible="true" />
+            <filter>(include['barcode_files']=='yes')</filter>
+        </data>
+    </xml>
+    <token name="@tool-documentation@">
+<![CDATA[
+
+Note: Output from Architecture with could have a Barcode HMM building block choose to find the barcode files.
+....If no barcode files are found this will simply be empty.
+....You may have refresh the history for all barcode files to show.
+
+Please contact the admin to add an architecture / HMM building blocks. 
+(There is Data Manager that they can use)
+
+]]>
+    </token>
+    <token name="@tagdust-documentation@">
+<![CDATA[
+====
+
+Taken from The TagDust2 Manual http://tagdust.sourceforge.net (part of Version 2_31 download)
+
+Raw sequences produced by next generation sequencing (NGS) machines can contain adapter, linker, 
+barcode and fingerprint sequences. TagDust2 is a program to extract and correctly label the sequences
+to be mapped in downstream pipelines.
+TagDust allows users to specify the expected architecture of a read and converts it into a hidden
+Markov model. The latter can assign sequences to a particular barcode (or index) even in the presence
+of sequencing errors. Sequences not matching the architecture (primer dimers, contaminants etc.) are
+automatically discarded
+
+TagDust requires an input file containing sequences and a user defined HMM architecture used to ex-
+tract the reads. The architecture is composed of a selection of pre-defined building blocks representing
+indices, barcodes, spacers and other sequences one might encounter in the raw output of a sequenced
+sample.
+
+HMM Building Blocks
+
+TagDust comes with a set of pre-defined HMM building blocks. Each includes a silent state at the
+beginning and end used to link blocks together. Each block is specified by a unique letter following
+by a colon and some information about the sequence.
+
+Read
+Segment modeling the read.
+Code: R:N
+
+Optional
+Segment modeling an optional single or short stretch of nucleotides.
+Code: O:N
+
+G addition
+Segment modeling the occasional addition of guanines to the reads. 
+(89.3% chance of a single G , 19.5% chance of 2 Gs..).
+Code: G:G
+
+Barcode or Index
+Segment modeling a set of barcode sequences. For each sequence a separate HMM is created. The
+barcode sequences must be given as a comma separated list. A null model of the same length as the
+barcode is automatically added and initialized to the background nucleotide frequencies.
+Code: B:GTA,AAC
+
+Fingerprint or Unique Molecular Identifier - UMI
+Segment modeling a fingerprint (or unique molecular identifiers). Insertions and deletions are by
+default not allowed within a fingerprint segment.
+Code: F:NNN
+
+Spacer
+Segment modeling a pre-defined sequence.
+Code: S:GTA
+
+Partial
+This segment is used to model sequences that may only be partially present at the 5‘ or 3‘ end of
+the read. The transition probabilities (orange and blue) are set automatically based on the length
+distribution of exactly matching adapters.
+Code: P:CCTTAA
+]]>
+    </token>
+    <xml name="citations">
+        <citations>
+        <citation type="bibtex">
+            @misc{
+                TagDust,
+                author = {Timo Lassmann},
+                title = {TagDust on sourceforge},
+                url = {http://tagdust.sourceforge.net/}
+            }
+        </citation>
+            <citation type="doi">10.1093/bioinformatics/btp527</citation>
+            <citation type="doi">10.1186/s12859-015-0454-y</citation>
+        </citations>
+    </xml>
+</macros>
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/paired.xml	Mon May 09 04:08:01 2016 -0400
@@ -0,0 +1,95 @@
+<tool id="tagdust_paired_runner_2_31" name="Tagdust paired end runner" version="0.2">
+    <description>Runs tagdust in paired end mode</description>
+    <macros>
+        <import>macros.xml</import>
+    </macros>
+    <expand macro="requirements"></expand>
+    <expand macro="stdio"></expand>
+    <expand macro="version_command"></expand>
+    <command >
+        @cat-archiecture@
+        cp $read1_file read1.fastq ;
+        cp $read2_file read2.fastq ;
+        @tagdust-call-minus-files@
+            read1.fastq read2.fastq ;
+        @ls_and_barcode@
+    </command>
+    <inputs>
+        <param name="read1_file" type="data" format="fastq,fastqsanger" label="Read 1 Fastq file to process" />
+        <param name="read2_file" type="data" format="fastq,fastqsanger" label="Read 2 Fastq file to process" />
+        <expand macro="reference_source"/>
+        <expand macro="architecture"/>
+        <expand macro="include_barcode_file"/>
+        <expand macro="architecture"></expand>
+    </inputs>
+    <outputs>
+        <data format="txt" name="logfile" label="Tagdust log on ${on_string}." from_work_dir="output/data_logfile.txt"/>
+        <data format_source="read1_file" name="extracted1" label="Tagdust extracted Read1 from ${on_string}." from_work_dir="output/data_READ1.fq"/>
+        <data format_source="read1_file"  name="unextracted1" label="Tagdust un-extracted Read1 from ${on_string}." from_work_dir="output/data_un_READ1.fq"/>
+        <data format_source="read2_file"  name="extracted2" label="Tagdust extracted Read2 from ${on_string}." from_work_dir="output/data_READ2.fq"/>
+        <data format_source="read2_file"  name="unextracted2" label="Tagdust un-extracted Read2 from ${on_string}." from_work_dir="output/data_un_READ2.fq"/>
+        <expand macro="output_barcode"/>
+    </outputs>
+    <tests>
+        <!-- Large dataset in github but not toolshed -->
+        <test>
+            <param name="read1_file" value="C75_S29_R1_001_extracted.fastq" ftype="fastq" /> 
+            <param name="read2_file" value="C75_S29_R2_001_extracted.fastq" ftype="fastq" /> 
+            <param name="architecture" value="R:N" /> 
+            <param name="reference_source|reference_source_selector" value="history"/>
+            <param name="reference_source|ref_file" value="ercc_and_TPA_mouse_rRNA.fa" ftype="fasta"/>
+            <output name="logfile" >
+                <assert_contents>
+                    <has_text text="Determining threshold for read0." />
+                 </assert_contents>
+            </output>
+            <output name="extracted1" file="C75_S29__READ1.fq" compare="sim_size" delta="10000"/>
+            <output name="unextracted1" file="C75_S29__un_READ1.fq"  compare="sim_size" delta="10000"/>
+            <output name="extracted2" file="C75_S29__READ2.fq" compare="sim_size" delta="10000"/>
+            <output name="unextracted2" file="C75_S29__un_READ2.fq"  compare="sim_size" delta="10000"/>
+        </test>
+        <test>
+            <param name="read1_file" value="read1_extracted.fastq" ftype="fastqsanger" /> 
+            <param name="read2_file" value="read2_extracted.fastq" ftype="fastqsanger" /> 
+            <param name="architecture" value="test_R:N" /> 
+            <param name="reference_source|reference_source_selector" value="history"/>
+            <param name="reference_source|ref_file" value="ercc_and_TPA_mouse_rRNA.fa" ftype="fasta"/>
+            <output name="logfile" >
+                <assert_contents>
+                    <has_text text="Determining threshold for read0." />
+                 </assert_contents>
+            </output>
+            <output name="extracted1" file="paired_READ1.fq" ftype="fastqsanger" compare="sim_size" delta="1000"/>
+            <output name="unextracted1" file="paired_un_READ1.fq"  ftype="fastqsanger" compare="sim_size" delta="1000"/>
+            <output name="extracted2" file="paired_READ2.fq" ftype="fastqsanger" compare="sim_size" delta="1000"/>
+            <output name="unextracted2" file="paired_un_READ2.fq" ftype="fastqsanger" compare="sim_size" delta="1000"/>
+        </test>
+        <test>
+            <param name="read1_file" value="read1_extracted.fastq" ftype="fastq" /> 
+            <param name="read2_file" value="read2_extracted.fastq" ftype="fastq" /> 
+            <param name="architecture" value="test_R:N" /> 
+            <param name="reference_source|reference_source_selector" value="cached"/>
+            <param name="ref_path" value="test_ercc_and_TPA_mouse_RNA"/>
+            <output name="logfile" >
+                <assert_contents>
+                    <has_text text="Determining threshold for read0." />
+                 </assert_contents>
+            </output>
+            <output name="extracted1" file="paired_READ1.fq" compare="sim_size" delta="1000"/>
+            <output name="unextracted1" file="paired_un_READ1.fq"  compare="sim_size" delta="1000"/>
+            <output name="extracted2" file="paired_READ2.fq" compare="sim_size" delta="1000"/>
+            <output name="unextracted2" file="paired_un_READ2.fq"  compare="sim_size" delta="1000"/>
+        </test>
+    </tests>
+    <help>
+<![CDATA[
+This tool runs Tagdust2 in paired end mode.
+
+]]>
+@tool-documentation@
+
+@tagdust-documentation@
+
+    </help> 
+    <expand macro="citations"></expand>
+</tool>
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/single.xml	Mon May 09 04:08:01 2016 -0400
@@ -0,0 +1,87 @@
+<tool id="tagdust_single_runner_2_31" name="Tagdust single end runner" version="0.2">
+    <description>Runs tagdust in single end mode</description>
+    <macros>
+        <import>macros.xml</import>
+    </macros>
+    <expand macro="requirements"/>
+    <expand macro="stdio"/>
+    <expand macro="version_command"/>
+    <command >
+        @cat-archiecture@
+        cp $fastq_file input.fastq ;
+        @tagdust-call-minus-files@
+            input.fastq ;
+        @ls_and_barcode@
+    </command>
+    <inputs>
+        <param name="fastq_file" type="data" format="fastq,fastqsanger" label="Fastq file to process" />
+        <expand macro="reference_source"/>
+        <expand macro="architecture"/>
+        <expand macro="include_barcode_file"/>
+    </inputs>
+    <outputs>
+        <data format="txt" name="logfile" label="Tagdust log on ${on_string} ." from_work_dir="output/data_logfile.txt"/>
+        <data format_source="fastq_file" name="extracted" label="Tagdust extracted reads from ${on_string}." from_work_dir="output/data.fq"/>
+        <data format_source="fastq_file" name="unextracted" label="Tagdust un-extracted reads from ${on_string}." from_work_dir="output/data_un.fq"/>
+        <expand macro="output_barcode"/>
+    </outputs>
+    <tests>
+        <!-- Large dataset in github but not toolshed -->
+        <!--test>
+            <param name="fastq_file" value="C75_S29_R1_001.fastq" ftype="fastq" /> 
+            <param name="architecture" value="F:NNNNNNNN_S:TATAGGG_R:N" /> 
+            <param name="include|barcode_files" value="no" /> 
+            <output name="logfile" >
+                <assert_contents>
+                    <has_text text="Determining threshold for read0." />
+                 </assert_contents>
+            </output>
+            <output name="extracted" file="C75_S29_R1_001.fq" compare="sim_size" delta="10000"/>
+            <output name="unextracted" file="C75_S29_R1_001_un.fq"  compare="sim_size" delta="10000"/>
+        </test-->
+        <test>
+            <param name="fastq_file" value="read1.fastq" ftype="fastqsanger" /> 
+            <param name="architecture" value="test_F:NNNNNNNN_S:TATAGGG_R:N" /> 
+            <param name="barcode_files" value="no" /> 
+            <output name="logfile" >
+                <assert_contents>
+                    <has_text text="Determining threshold for read0." />
+                 </assert_contents>
+            </output>
+            <output name="extracted" file="read1.fq"  ftype="fastqsanger" compare="sim_size" delta="1000"/>
+            <output name="unextracted" file="read1_un.fq"  ftype="fastqsanger" compare="sim_size" delta="1000"/>
+        </test>
+        <test>
+            <param name="run_type_selector" value="single"/> 
+            <param name="fastq_file" value="read1.fastq" ftype="fastq" /> 
+            <param name="architecture" value="test_B:GTA_AAC_R:N" /> 
+            <param name="include|barcode_files" value="yes" /> 
+            <output name="logfile" >
+                <assert_contents>
+                    <has_text text="Determining threshold for read0." />
+                 </assert_contents>
+            </output>
+            <output name="extracted" file="with_b_.fq"/>
+            <output name="unextracted" file="with_b_un.fq"/>
+            <output name="barcode_files">
+                <assert_contents>
+                    <has_line line="data_BC_AAC.fq" />
+                    <has_line line="data_BC_GTA.fq" />
+                </assert_contents>
+                <discovered_dataset designation="AAC" ftype="fastq" file="with_b_BC_AAC.fq"/>
+                <discovered_dataset designation="GTA" ftype="fastq" file="with_b_BC_GTA.fq"/>
+            </output>
+        </test>
+    </tests>
+    <help>
+<![CDATA[
+This tool runs Tagdust2 in single mode.
+
+]]>
+@tool-documentation@
+
+@tagdust-documentation@
+
+    </help> 
+    <expand macro="citations"></expand>
+</tool>
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/paired_READ1.fq	Mon May 09 04:08:01 2016 -0400
@@ -0,0 +1,216 @@
+@NB500968:8:H5CFGAFXX:1:11101:1121:1139___1:N:0:TCGTGAGC+TACTCCTT;FP:27828;RQ:34.83;RQ:-1.00
+GGAGCGTCAGATGTGTATAAGAGACAGGTATTCCCTATAGTCTACATC
++
+EEEEEEEEEEEEEEEAAEEEEAEEEAEEE/EEE/EE<AAEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:7529:1175___1:N:0:TCCTGAGC+TACTCCTT;FP:17358;RQ:34.81;RQ:-1.00
+AGCGTCAGATGTGTATAAGAGACAGCAACCCTAAAAATATCCA
++
+EEEE/EEEEEEA/EA<EEEE/AEEEE//E//6A/AE/A/AAE/
+@NB500968:8:H5CFGAFXX:1:11101:5052:1198___1:N:0:TCCTGAGC+TACTCCTT;FP:38799;RQ:34.63;RQ:-1.00
+GGTTTTTAGTGGCATTCATTGTGTGGATTCCTCTGTTGCTGCCCCTGGGCTAACAAGGGT
++
+EEEEEEE6EEEEAEEEEEEEEEEEAEEEEEEEEEEEEEAAE/AEEEEEAAAEEEE/EAEE
+@NB500968:8:H5CFGAFXX:1:11101:15805:1205___1:N:0:TCCTGAGC+TACTCCTT;FP:44542;RQ:34.63;RQ:-1.00
+GAGGGCCAGGCAGCTCAAAGCCTGAAGGAGGTACTTCAAGGAGGGTCCAGAGAAGCTGGG
++
+EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEE<EAAEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:11834:1217___1:N:0:TCCTGAGC+TACTCCTT;FP:56795;RQ:34.81;RQ:-1.00
+AGCGTCAGATGTGTATAAGAGACAGGATTCCCCTATATAGCGACCC
++
+EEEEEEEEEEEEEEEEAEEEEEEEEEEAEEEEEEEEEA<EAEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:20105:1230___1:N:0:TCCTGAGC+TACTCCTT;FP:2265;RQ:34.63;RQ:-1.00
+GTTGTTTCATGTTGCAATTTTTAGGTTACTTTTGAGTTGCTTAGGAATTGTTTTATTTAA
++
+EEEEEEEEEE/E/AEEEEE<E</EEAEEEEEEAAEAEEEAAE//EA//</E6EAAEA/6/
+@NB500968:8:H5CFGAFXX:1:11101:14524:1264___1:N:0:TCCTGAGC+TACTCCTT;FP:7937;RQ:2.65;RQ:-1.00
+CCCAACTTAATATTATAAAATTATCATAAAAACAACTACGAGCCCACGATACAACTGATA
++
+//EEEE<///A/AAA/A////E/<///E/6/E/E/E6///E//6E<//6/AA//E//A//
+@NB500968:8:H5CFGAFXX:1:11101:14981:1265___1:N:0:TCCTGAGC+TACTCCTT;FP:26333;RQ:34.63;RQ:-1.00
+GTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCG
++
+EEEEEEEEEEEEEAEEEEEEEEEAAEEEEEEEA<EEEEEEEEE6/E66A<A/<AA<A//6
+@NB500968:8:H5CFGAFXX:1:11101:5538:1286___1:N:0:TCCTGAGC+TACTCCTT;FP:5079;RQ:34.62;RQ:-1.00
+AGTGAACTAACTAGAAAACTTCTAACTAAAAGAATTACAGCTAGAAACCCCGAAACCAAA
++
+AAEEEEEEEEAE/AEEEEEEEAEEEEE/AE6/EEA<E/EA6EAAEEE6//6<<EEA/<<A
+@NB500968:8:H5CFGAFXX:1:11101:12411:1441___1:N:0:TCCTGAGC+TACTCCTT;FP:46039;RQ:34.83;RQ:-1.00
+GGGAGCGTCAGATGTGTATAAGAGACAGTCTCCCTATATTTTGGC
++
+//AEEE/EAEAEE/AEAE<EEEEAE/EAE<E/E</EEEE/6E/EE
+@NB500968:8:H5CFGAFXX:1:11101:5889:1458___1:N:0:TCCTGAGC+TACTCCTT;FP:52002;RQ:34.83;RQ:-1.00
+GAGCGTCAGATGTGTATAAGAGACAGCGTATCCCTATACCCCTTCGCTG
++
+EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:9851:1471___1:N:0:TCCTGAGC+TACTCCTT;FP:43356;RQ:3.17;RQ:-1.00
+CCCCTGTCTCTTATACCNNN
++
+EEEEEA/AA/EEEEE//###
+@NB500968:8:H5CFGAFXX:1:11101:5425:1488___1:N:0:TCCTGAGC+TACTCCTT;FP:13390;RQ:34.83;RQ:-1.00
+GGAGCGTCAGATGTGTATAAGAGACAGACTCCCCTATATGGACACA
++
+EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:8024:1581___1:N:0:TCCTGAGC+TACTCCTT;FP:38311;RQ:34.63;RQ:-1.00
+GTGACCCGCCGCTTCAGGGACCCGCTTCTCTCATCTTCCCCAAAGTTCTGGACAGATGAT
++
+EEEEEEEAEEEEEAEAEEEEEEEEEEAEEEEAEEEAEAEEEEEEEEAEEEEEEEEEE/A<
+@NB500968:8:H5CFGAFXX:1:11101:23474:1673___1:N:0:TCCTGAGC+TACTCCTT;FP:2968;RQ:34.81;RQ:-1.00
+AGCGTCAGATGTGTATAAGAGACAGAGGCGCCCTATAGAATATAT
++
+EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEE/EEEEE
+@NB500968:8:H5CFGAFXX:1:11101:21720:1786___1:N:0:TCCTGAGC+TACTCCTT;FP:16257;RQ:35.14;RQ:-1.00
+GGGAGCGTCAGATGTGTATAAGAGAC
++
+EEEEEEEEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:9012:1809___1:N:0:TCCTGAGC+TACTCCTT;FP:4167;RQ:34.83;RQ:-1.00
+GGGAGCGTCAGATGTGTATAAGAGACAGGTACCCCCTATATCATAGGC
++
+EEEEEEEEEEEEEEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:20110:1819___1:N:0:TCCTGAGC+TAATCCTT;FP:64559;RQ:34.63;RQ:-1.00
+GGATTCTTGAAGTATAACAGGAGAATCAAATAATTAAAGAGGGGGCTGGAGAGATAGCT
++
+EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:24842:1887___1:N:0:TCCTGAGC+TACTCCTT;FP:13772;RQ:35.14;RQ:-1.00
+GGGAGCGTCAGATGTGTATAAGAG
++
+EEEEEEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:16709:1904___1:N:0:TCCTGAGC+TACTCCTT;FP:5671;RQ:34.73;RQ:-1.00
+GTTCCATGCGTCAATGTTTCTTCTGGATACAGCAGGCCTGTTCCCGGGAGTCAATGC
++
+EEEEEAEEEEEEEEEEAEEEEEEEEEEEAEE6<EEEEEAAA<EAEEEEEEEEEEE<E
+@NB500968:8:H5CFGAFXX:1:11101:12189:1919___1:N:0:TCCTGAGC+TACTCCTT;FP:53204;RQ:34.62;RQ:-1.00
+ACACCCTTTATAGTGATGGATTGGATCTGTTTGAAGGCAAAGTCCTTCCCAGACTTATCA
++
+EEEEEEE/EEE/EEAEEEEEEEEEEE/EE<EAAAE/E<</EA///A</E/A/AAA<A/</
+@NB500968:8:H5CFGAFXX:1:11101:17381:2018___1:N:0:TCCTGAGC+TACTCCTT;FP:64754;RQ:35.18;RQ:-1.00
+AACGTCAGATGTGTATAAGN
++
+EEE/EEEAEEEEEAE</EA#
+@NB500968:8:H5CFGAFXX:1:11101:9520:2027___1:N:0:TCCTGAGC+TACTCCTT;FP:57159;RQ:34.81;RQ:-1.00
+AGCGTCAGATGTGTATAAGAGACAGCTACATCCCTATAAGGATGTAC
++
+EEEEEEEEEEEEEEEEAEEEEEEEEEEAEEEEEEE/EEEEE<AEEEA
+@NB500968:8:H5CFGAFXX:1:11101:19677:2089___1:N:0:TCCTGAGC+TACTCCTT;FP:14776;RQ:34.63;RQ:-1.00
+GGCGCCAGTCCTCCGATTGACTGAGTCGCCCGGGTACCCGTGTATCCAATAAACCCTCTT
++
+EEEEEEEAEEEEEEEAE/EEEEEEEEAEEE//E/EEE/A<AAAAAA<AEAE/EE/AAA<6
+@NB500968:8:H5CFGAFXX:1:11101:26158:2095___1:N:0:TCCTGAGC+TACTCCTT;FP:23868;RQ:34.83;RQ:-1.00
+GGGAGCGTCAGATGTGTATAAGAGACAGCCACTCCCTATAGGATTTCC
++
+EEEEEEEEEAEEEEEEEEEEEEEEEEEEA<EEEEAEEA<6EEEEE<EA
+@NB500968:8:H5CFGAFXX:1:11101:7883:2101___1:N:0:TCCGGAGC+TACTCCTT;FP:59501;RQ:34.83;RQ:-1.00
+GGAGCGTCAGATGTGTATAAGAGACAGCATCACCCTATAGGCTGTCT
++
+EEEEEEEEEEEEEEAEAEEEEEEEEEEEEEEEEEEEEE<EEEEEEAE
+@NB500968:8:H5CFGAFXX:1:11101:24496:2131___1:N:0:TCCTGAGC+TACTCCTT;FP:247;RQ:35.19;RQ:-1.00
+GGGAGCGTCAGATGTGTATAAG
++
+EEEEEEEEEEE/EEEEEEE/AE
+@NB500968:8:H5CFGAFXX:1:11101:5814:2162___1:N:0:TCCTGAGC+TACTCCTT;FP:5751;RQ:34.63;RQ:-1.00
+GCCCTTGGCTTCACCACACTTTCTACTGGAAATGTGCCTTTTCCTCCATTGCTGCTAC
++
+EEEEEEEEEEEEEEEAAEEEAEEEEEA/EEEEEE<EEEAEAAEEAEEE<AAEAEEAAE
+@NB500968:8:H5CFGAFXX:1:11101:5796:2184___1:N:0:TCCTGAGC+TATTCCTT;FP:4250;RQ:34.83;RQ:-1.00
+GGAGCGTCAGATGTGTATAAGAGACAGGCTGTCCCTATAGACTGACT
++
+EEEEEEEEEEEEEEEAEAEEEEEEEEEEEEEEEEAEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:23937:2223___1:N:0:TCCTGAGC+TACTCCTT;FP:26022;RQ:34.63;RQ:-1.00
+GCTTCTTCTGAAGAGCTCCACCTGTGGTCTCTCCACCTCTCGAGGCTCTTCACTAGCCT
++
+EEEEAEA/AEEEEEEEA/</</</A//EAEEE<AE66A666/A/E6E/AAAAA////E/
+@NB500968:8:H5CFGAFXX:1:11101:25267:2246___1:N:0:TCCTGAGC+TACTCCTT;FP:4173;RQ:34.83;RQ:-1.00
+GAGCGTCAGATGTGTATAAGAGACAGGATGCCCCTATATATAGAGGCTG
++
+E6EEEEAAEEEEEEEAEEEEEEEEEEEEEEE6EEEEEEEEEAEAEEAEA
+@NB500968:8:H5CFGAFXX:1:11101:6670:2262___1:N:0:TCCTGAGC+TACTCCTT;FP:14539;RQ:3.28;RQ:-1.00
+GGAGGCCAAATTGTGCTGATTTTCCTGTAGCTGCGATTAATAGGCCTATAAGTGGAATTA
++
+EEEEEEEEEEAEAEEAEEEEEEEE/EAEEEAEEAEEAE/EEAE/</E/EA66A<//<EE/
+@NB500968:8:H5CFGAFXX:1:11101:14858:2304___1:N:0:TCCTGAGC+TACTCCTT;FP:4202;RQ:34.63;RQ:-1.00
+GTCTCCGGCTTGTGTAGACTCTGCCTGGAAGTGAAGTTGTGGACTCCCGACCTCCAGTC
++
+EEEEEEEEEEEEEEEEEEEEEA/E/AEEEEEAEEEEAE/<AAEEE<EAEEE<EEAEEEE
+@NB500968:8:H5CFGAFXX:1:11101:10463:2345___1:N:0:GCCTGAGC+TACTCCTT;FP:24337;RQ:34.81;RQ:-1.00
+TCCAGCGTCAGATGTGTATAAGAGACAGAGTTCCCCTATAGAGAGACG
++
+EEEEEEEEEEE6EEEEA/A//E/EEEEEEEEEEEEEEAA/AAEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:24405:2351___1:N:0:TCCTGAGC+TACTCCTT;FP:5580;RQ:35.18;RQ:-1.00
+TCTGTCTCTTATACCNNNNN
++
+EEEEEEAEEEEEEEE#####
+@NB500968:8:H5CFGAFXX:1:11101:6508:2370___1:N:0:TCCTGAGC+TACTCCTT;FP:64422;RQ:34.83;RQ:-1.00
+GGAGCGTCAGATGTGTATAAGAGACAGCCCAGCCCTATACCGTCTAGCTG
++
+EEEEEEEEEEEEEAEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:21730:2488___1:N:0:TCGTGAGC+TACTCCTT;FP:57284;RQ:35.18;RQ:-1.00
+AGCGTCAGATGTGTATCNNN
++
+EEEEEEEEEEEEEAEAE###
+@NB500968:8:H5CFGAFXX:1:11101:20021:2516___1:N:0:TCCTGGGC+TACTCCTT;FP:28351;RQ:34.83;RQ:-1.00
+GGAGCGTCAGATGAGTATAAGAGACAGTCATCCCCTATAGTCGTTCA
++
+EAEEAAEEAAE///E/EAAE/EAEE//EEE/EEEEEE/EE/EA6EEE
+@NB500968:8:H5CFGAFXX:1:11101:4827:2579___1:N:0:TCCTGAGC+TACTCCTT;FP:8626;RQ:34.83;RQ:-1.00
+GACAGCGTCAGATGTGTATAAGAGACAGGGAATCCCTATAGACTTTGC
++
+EEEEEEEEE/E<AAAEEEEEEEE/E/EEEEEEEEEEEAEEAEAAEEE/
+@NB500968:8:H5CFGAFXX:1:11101:11758:2662___1:N:0:TCCTGAGC+TACTCCTT;FP:32308;RQ:34.83;RQ:-1.00
+GACAGCGTCAGATGTGTATAAGAGACAGAGATGCCCTATAGACCGAAC
++
+EEEEEE/EAE/EEE/EEEAEE6E6EEEEEAAEA/E/AEEEA/E6EA/E
+@NB500968:8:H5CFGAFXX:1:11101:23131:2747___1:N:0:TCCTGAGC+TACTCCTT;FP:61606;RQ:34.61;RQ:-1.00
+CAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCGAGGTTATCTA
++
+EEEEEEAEAEEEE/EEEAEEEEA/EEEEAEA/EA<EAAAEEAE<AE/A<<EEEA<<EEA<
+@NB500968:8:H5CFGAFXX:1:11101:20373:2751___1:N:0:TCCTGATC+TACTCCTT;FP:283;RQ:34.93;RQ:-1.00
+GGAGCGTCAGATGTGTATAAGAGACAGCCCTATACAGTGATC
++
+EEEEEEEEE<EEEEAEAEEEEEEEEEEEEEE/EEEEEE<EAA
+@NB500968:8:H5CFGAFXX:1:11101:21204:2795___1:N:0:TCCTGAGC+TACTCCTT;FP:16279;RQ:34.81;RQ:-1.00
+AGCGTCAGATGTGTATAAGAGACAGCTCCACCCTATAGGCCCTAACTG
++
+EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:17699:2880___1:N:0:TCCTGAGC+TACTCCTT;FP:52710;RQ:34.62;RQ:-1.00
+CTTTGGAATCTTCACAAGCAGAACCAGTCAAAACCCCGGCAAGCACAAAGAGACTCTCCA
++
+EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE<EEEEEEEEEEE6AEAEAEEEEA
+@NB500968:8:H5CFGAFXX:1:11101:2261:2941___1:N:0:TCCTGAGC+TACTCCTT;FP:2596;RQ:34.63;RQ:-1.00
+GGCAGCATCAGATGTGTATAAGAGACAGAAGGAGCAGAGAGCGGCCAGCAACCGATGAGA
++
+EEEEEEEEEEEEEEAEEEAEEEEE///////EA//E/E/A/////E<EE//</E<<////
+@NB500968:8:H5CFGAFXX:1:11101:25690:2959___1:N:0:TCCTGCGC+TACTCCTT;FP:27004;RQ:34.81;RQ:-1.00
+AGCGTCAGATGTGTATAAGAGACAGGCCTCCCCTATAGCTTCGGA
++
+AE6EEEEEEEEEAAEEEEEEEEEEEEEEAEE/E/EAEEEEAEEEE
+@NB500968:8:H5CFGAFXX:1:11101:8090:2972___1:N:0:TCCTGAGC+TACTCCTT;FP:12902;RQ:34.81;RQ:-1.00
+CAACGTCAGATGTGTATAAGAGACAGGGACCCCCTATAATTTATGCC
++
+EEEEEEEEEEEEEEEEEAAEEEEEEEEEAEE<EEEEEAE<EEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:16936:3211___1:N:0:TCCTGAGC+TACTCCTT;FP:7401;RQ:34.83;RQ:-1.00
+GAGCGTCAGATGTGTATAAGAGACAGTGCGTCCCTATAAAAGTGAACTG
++
+EEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEEEEEEA/EEEAEAEEE
+@NB500968:8:H5CFGAFXX:1:11101:6132:3257___1:N:0:TCCTGAGC+TACTCCTT;FP:7557;RQ:34.62;RQ:-1.00
+CGTCAGATGTGTATAAGAGACAGCATTCCCCTATACACGCCTCCTGTCTCTTATAC
++
+EEEEAEEEEEEEEEEEEEEAEAEEAEEEEAEEEEEEEEEEEEEEEEAE/EEEEEEA
+@NB500968:8:H5CFGAFXX:1:11101:3840:3285___1:N:0:TCCTGAGC+TACTCCTT;FP:16156;RQ:34.93;RQ:-1.00
+GGGAGCGTCAGATGTGTATAAGAGACAGGTATACCCTATAG
++
+EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:2858:3312___1:N:0:TCCTGAGC+TACTCCTT;FP:14452;RQ:35.12;RQ:-1.00
+AACGTCAGATGTGTATAAGAG
++
+EE/AE/E/EAAEEEEEEEAEE
+@NB500968:8:H5CFGAFXX:1:11101:3641:3398___1:N:0:TCCTGAGC+TACTCCTT;FP:36051;RQ:35.14;RQ:-1.00
+GCAGCGTCAGATGTGTATAAGAG
++
+EEEEEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:20540:3401___1:N:0:TCCTGAGC+TACTCCTT;FP:48603;RQ:34.81;RQ:-1.00
+AGCGTCAGATGTGTATAAGAGACAGATCAACCCTATATGCCCTAACTG
++
+E/E///E/E</A/EEA<E/E/EEE/E</E///A/E6<E/A/<E/EEE/
+@NB500968:8:H5CFGAFXX:1:11101:4772:3477___1:N:0:TCCTGAGC+AACTCCTT;FP:61788;RQ:3.33;RQ:-1.00
+TAACTGTCTCTTATACACCN
++
+EEEEEEEAEEEAEEE<EEE#
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/paired_READ2.fq	Mon May 09 04:08:01 2016 -0400
@@ -0,0 +1,216 @@
+@NB500968:8:H5CFGAFXX:1:11101:1121:1139___1:N:0:TCGTGAGC+TACTCCTT;FP:27828;RQ:34.83;RQ:-1.00
+GATGTAGACTATAGGGAATACCNNNNNNNNNNNNN
++
+A6AAAEEEEEEEEEEEAEE/E<#############
+@NB500968:8:H5CFGAFXX:1:11101:7529:1175___1:N:0:TCCTGAGC+TACTCCTT;FP:17358;RQ:34.81;RQ:-1.00
+GATGTGTAAAAGAGACAGAGGATAATTATAGGGATCCTGTCTCTAAAACACATCTGACGCTCCCTATACATAATA
++
+/AAAAE<A/E//EEE/EE//EEEEAEEEEEEEEEE/A/<EEE<<///<AE</AE//AAA//E/////EEE<6/A/
+@NB500968:8:H5CFGAFXX:1:11101:5052:1198___1:N:0:TCCTGAGC+TACTCCTT;FP:38799;RQ:34.63;RQ:-1.00
+GGATTGAGGCTAGAAACAGCTGCTTTTCTCACTTTCCCAGCAGCAAGACATCAATCTGTTGAGACCTCTCATTTT
++
+AA6AAEEAEEEEEEEEAAEEEEE///AEEA/<E/E<A//EEEEAE/EE//AE///E</EAEEA<EEEAEE/E/E/
+@NB500968:8:H5CFGAFXX:1:11101:15805:1205___1:N:0:TCCTGAGC+TACTCCTT;FP:44542;RQ:34.63;RQ:-1.00
+GCGTCAGATGTGGGTAGTCAGAAAAGCAGATGGATGGGGGCCTTGCCCAACCAGATCCAGGCCGAGTTCACCT
++
+AAAAAEEAEEEEEEEEEEEEEEA//EEEEEEEEEEEEEEEEEEAEE/E//EEEEAEAE/EEEEE<EEEEEEEA
+@NB500968:8:H5CFGAFXX:1:11101:11834:1217___1:N:0:TCCTGAGC+TACTCCTT;FP:56795;RQ:34.81;RQ:-1.00
+GGGTCGCTATATAGGGGAATCCNNNNNNNNNNNNN
++
+AAAAAEEEEEEEEEEEEEAEEE#############
+@NB500968:8:H5CFGAFXX:1:11101:20105:1230___1:N:0:TCCTGAGC+TACTCCTT;FP:2265;RQ:34.63;RQ:-1.00
+GTACACGTATTACTTCTGTTTGGTGAATTAAATAAAACAATTCCTAAGCAACTCAAAAGTAACCTAAAAATTGCA
++
+AA/A//EEE/EAEEEEEEE/EEEAEE6EEE<EEA<6E//E/EEAEE//EE/EEE<EAEE6</EEEEE/A/EEE/A
+@NB500968:8:H5CFGAFXX:1:11101:14524:1264___1:N:0:TCCTGAGC+TACTCCTT;FP:7937;RQ:2.65;RQ:-1.00
+GTATAAGAGACAGTTGGGACTTATAGGGTAAGTCN
++
+AAAAAEEEAEEEEAEEE/EEEEEAE<EE/EEEEE#
+@NB500968:8:H5CFGAFXX:1:11101:14981:1265___1:N:0:TCCTGAGC+TACTCCTT;FP:26333;RQ:34.63;RQ:-1.00
+GGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCG
++
+AA6AAEE/EE//EEE6EEEEEEEE/AAEEAEEEE/EEEEEAEEEE/EEEEEEEEEEEE/AEE<EEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:5538:1286___1:N:0:TCCTGAGC+TACTCCTT;FP:5079;RQ:34.62;RQ:-1.00
+ATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCACTATTTTGCCACATAGACGAGTTGATT
++
+AAA/AEE/EEEEEEEAEEEEEEEE/EEEEE/EEEA</EEEEEEEEAAAEAEEEEE<EAE6EEEEEEEEEEEEAE
+@NB500968:8:H5CFGAFXX:1:11101:12411:1441___1:N:0:TCCTGAGC+TACTCCTT;FP:46039;RQ:34.83;RQ:-1.00
+GACAAAATATAGGGGGACTGTCTGTTATAGACGTCGGACGCGCGCCCCTATAAGGAATAGGGGTCTCTGAGAGA
++
+A/////E////////A///E/E///EEE///A//A/A//<///////E/////AEA//////////////////
+@NB500968:8:H5CFGAFXX:1:11101:5889:1458___1:N:0:TCCTGAGC+TACTCCTT;FP:52002;RQ:34.83;RQ:-1.00
+CAGCGAAGGGGTATAGGGATACGNNNNNNNNNNNN
++
+A/AAA//EEEEA/A/EEE////E############
+@NB500968:8:H5CFGAFXX:1:11101:9851:1471___1:N:0:TCCTGAGC+TACTCCTT;FP:43356;RQ:3.17;RQ:-1.00
+GTATAAGAGACAGGGGCCCGTTAAAGGGCCCNNNN
++
+/A6<//EA//E/E/A/EEE/E/E/E/6EEE/####
+@NB500968:8:H5CFGAFXX:1:11101:5425:1488___1:N:0:TCCTGAGC+TACTCCTT;FP:13390;RQ:34.83;RQ:-1.00
+GTATAAGAGACAGTGTGTCCATATAGGGGAGTCNN
++
+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEE##
+@NB500968:8:H5CFGAFXX:1:11101:8024:1581___1:N:0:TCCTGAGC+TACTCCTT;FP:38311;RQ:34.63;RQ:-1.00
+GAACTATGACTGTGAGCAGGCATTGTGGTTCTGCCCAAGCTACATCCAGATCACCCAGGCCTCTGGGTCTTTTGG
++
+AAAA6EAEE/E/EEEEEEEAEEEEEEEAEEEEAEEEEEAE6EEE6EEEAA/EEEEEE/A/E/AEAEE<EEEEA/<
+@NB500968:8:H5CFGAFXX:1:11101:23474:1673___1:N:0:TCCTGAGC+TACTCCTT;FP:2968;RQ:34.81;RQ:-1.00
+GATGTGTATAAGAGACAGATATAGACTATAGGGCGCCT
++
+A/AA////A//EA/6<EE6/EEE6////A//EE/E6//
+@NB500968:8:H5CFGAFXX:1:11101:21720:1786___1:N:0:TCCTGAGC+TACTCCTT;FP:16257;RQ:35.14;RQ:-1.00
+GTCTCTTATACACATCTGACGCTCCCCCCTATAGTTCAAAT
++
+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:9012:1809___1:N:0:TCCTGAGC+TACTCCTT;FP:4167;RQ:34.83;RQ:-1.00
+GTGTATAAGAGACAGGCCTATGATATAGGGGGTAC
++
+AAAAAEEEEEEEEEEEEEAEE/EEEAE/AAEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:20110:1819___1:N:0:TCCTGAGC+TAATCCTT;FP:64559;RQ:34.63;RQ:-1.00
+GAAGAGGGTATCAGATCCCTTGGATCTGGAGTCGCAGATGGCTGCGAGCCACCATGTGGGTTTTGAGAATTGAAG
++
+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEEEAEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:24842:1887___1:N:0:TCCTGAGC+TACTCCTT;FP:13772;RQ:35.14;RQ:-1.00
+CTCATAAACACATCAGGCGGACCCCGCAAAATAAAGGATCTATCTCTGATAAAGAACTGACGCTGCCGACGAAA
++
+A///AE/6EE///////E//6E/AE/<////EE/A/A/A////EAA///////////E/EE/E/////A//E/A
+@NB500968:8:H5CFGAFXX:1:11101:16709:1904___1:N:0:TCCTGAGC+TACTCCTT;FP:5671;RQ:34.73;RQ:-1.00
+CGGATCGCTTCTTCTGATTGAGTTGCCACTCTTTGAAATGCGGCACGGCCTCGTCCACGTTGACGATGCCCAG
++
+AAAAAEEEEEEEAEEEEEEEEEEEEEEEEEEE/EEEEAEEEEEEEEEEEEEEEEE//EEEEEEEAEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:12189:1919___1:N:0:TCCTGAGC+TACTCCTT;FP:53204;RQ:34.62;RQ:-1.00
+GTCTATAGGTATCAGTTTCACTGTAATACCTTTCCTTCCATGGCTAAGTCTGGGAAGGACTTTGCCTTCAAACAG
++
+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE<AEEEEEEEEE<E<EAEAEEEEEEEEEEA
+@NB500968:8:H5CFGAFXX:1:11101:17381:2018___1:N:0:TCCTGAGC+TACTCCTT;FP:64754;RQ:35.18;RQ:-1.00
+CTTATACACATCTGACGTTCCCTATACTAATAAAN
++
+A/AAAAE6E////E/AEAE/AEEAEA/EA/EEEE#
+@NB500968:8:H5CFGAFXX:1:11101:9520:2027___1:N:0:TCCTGAGC+TACTCCTT;FP:57159;RQ:34.81;RQ:-1.00
+GTACATCCTTATAGGGATGTAGCNNNNNNNNNNNN
++
+AAAAAEEEEAEEEEEEEEAEEEE############
+@NB500968:8:H5CFGAFXX:1:11101:19677:2089___1:N:0:TCCTGAGC+TACTCCTT;FP:14776;RQ:34.63;RQ:-1.00
+CTCCTCTAAGGGGGGTGCACCAAAGAGTCCAAAACGATCGGGATTTTTGGACTCAGGTCGGGCCACAAAAACGG
++
+AAAAAA/EEEEEEEEEEE/EEEAEEEEEEE/AAAEA/<EEEEE/E</<<E/EEEE/E6EEAEAE/EEE///EEA
+@NB500968:8:H5CFGAFXX:1:11101:26158:2095___1:N:0:TCCTGAGC+TACTCCTT;FP:23868;RQ:34.83;RQ:-1.00
+GATGTGTATAAGAGACAGGGAAATCCTATAGGGAGTGG
++
+AAAAAEEEEEEEEEEEAEEEAEAEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:7883:2101___1:N:0:TCCGGAGC+TACTCCTT;FP:59501;RQ:34.83;RQ:-1.00
+GTATAAGAGACAGAGACAGCCTATAGGGTGATGCN
++
+AAA/AEEE/EE/EAAEE//EEA/E/EAAEE/E6<#
+@NB500968:8:H5CFGAFXX:1:11101:24496:2131___1:N:0:TCCTGAGC+TACTCCTT;FP:247;RQ:35.19;RQ:-1.00
+CTTATACACATCTGACGCTCCCCCCTATAAGAATTTT
++
+AAAAAAEAAE//EEEEEEEE/EEEEEEEAAAEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:5814:2162___1:N:0:TCCTGAGC+TACTCCTT;FP:5751;RQ:34.63;RQ:-1.00
+CTCCTACAGGACAGAAGGACTCTGCTGCCTGTTGCCAAGGAAGGCAGAGCTAGCTGAACTGCGTGTAAAAACGGG
++
+AAA/AEA/EEEEEEEEEEE/EAEEAE<EEA/EAEAA<EEEE/EEEEAEAEEEAEEAEAEEAAAEAEEE/E/<A<<
+@NB500968:8:H5CFGAFXX:1:11101:5796:2184___1:N:0:TCCTGAGC+TATTCCTT;FP:4250;RQ:34.83;RQ:-1.00
+GTGTATAAGAGACAGAGTCAGTCTATAGGGACAGC
++
+AAAAAEEEEEEEEEEEEEEEEEAEEEEAEAEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:23937:2223___1:N:0:TCCTGAGC+TACTCCTT;FP:26022;RQ:34.63;RQ:-1.00
+GAGATGGTCAGTGCAGAGTGATCGCAGCTACCGGAAAAGAGTTAGCTAGCAATGGGAGCTGAATGAGGCCGTGGT
++
+AAAAAEEEEEEEEEE6EEE6EEEAEEEEE/EEEE/E/E/EEEAE<AAEEA/EEEAAEEEAAE/AA//EEEE/AEA
+@NB500968:8:H5CFGAFXX:1:11101:25267:2246___1:N:0:TCCTGAGC+TACTCCTT;FP:4173;RQ:34.83;RQ:-1.00
+CAGCCTCTATATATAGGGGCATCCNNNNNNNNNNN
++
+A/AAAAEEEEEEEEEAEEEE6EAE###########
+@NB500968:8:H5CFGAFXX:1:11101:6670:2262___1:N:0:TCCTGAGC+TACTCCTT;FP:14539;RQ:3.28;RQ:-1.00
+CCTCTATAACCGCATCGGAGACATCGGATTCATTTTAGCTATAGTTTGATTTTCCCTAAACATAAGCTCATGAGA
++
+AAAA/////EEEE///EE/E/<//E<EE/////<AE/AA<A6<EE/EE6<<E////<///</////A/E//AAE/
+@NB500968:8:H5CFGAFXX:1:11101:14858:2304___1:N:0:TCCTGAGC+TACTCCTT;FP:4202;RQ:34.63;RQ:-1.00
+GCACTGCAGAGGTCTCTAGACAGCACTCTCTTCCTCTCCATGGTCCTCTCTTCCTCCAGCGCTGAGTACTGCTG
++
+AAAAAEEAEEEEEEEEEE6/E/EE/EEEEEEEE/EEEEEEEEEAEEEE/EEEEEEEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:10463:2345___1:N:0:GCCTGAGC+TACTCCTT;FP:24337;RQ:34.81;RQ:-1.00
+GTGTATAAGAGACAGCGTCTCTGTATAGGGGAAGT
++
+AAAA6E66E/E/A/A/E//EE//A///EEEE///E
+@NB500968:8:H5CFGAFXX:1:11101:24405:2351___1:N:0:TCCTGAGC+TACTCCTT;FP:5580;RQ:35.18;RQ:-1.00
+GTATAAGAGACAGACCCTATATATAGGGTCNNNNN
++
+AAAAAEEEEEEEEEEEEEEEAEAEEEEEEE#####
+@NB500968:8:H5CFGAFXX:1:11101:6508:2370___1:N:0:TCCTGAGC+TACTCCTT;FP:64422;RQ:34.83;RQ:-1.00
+CAGCTAGACGGTATAGGGCTGGGCNNNNNNNNNNN
++
+A/AAA/E/EEE//A/EEEAAEEEA###########
+@NB500968:8:H5CFGAFXX:1:11101:21730:2488___1:N:0:TCGTGAGC+TACTCCTT;FP:57284;RQ:35.18;RQ:-1.00
+ATACACATCTGACGCTCCCTATATGTAAAGANNNN
++
+AA/A///E</E/EEAE////////AEA//E/####
+@NB500968:8:H5CFGAFXX:1:11101:20021:2516___1:N:0:TCCTGGGC+TACTCCTT;FP:28351;RQ:34.83;RQ:-1.00
+CTAAAAGAGACAGTGCACGACTTAAGGGGATGAGTGTCTCGTATACACGTCTGACGCTCCCGCTATAAAACCACG
++
+//////E/E/E/E////EE/EE///E/EA//////E/E/////E/A///A//E/<<////A/E<///////E//E
+@NB500968:8:H5CFGAFXX:1:11101:4827:2579___1:N:0:TCCTGAGC+TACTCCTT;FP:8626;RQ:34.83;RQ:-1.00
+ATGTGTATAAGAGACAGTCGAAGTCTATAGGGATTCC
++
+AAAAAE/EA/E/E///E/A/A/EE//EE/E/E/E/AE
+@NB500968:8:H5CFGAFXX:1:11101:11758:2662___1:N:0:TCCTGAGC+TACTCCTT;FP:32308;RQ:34.83;RQ:-1.00
+GTGTATAAGAGACAGGTTCGGTCTATAGGGCATCT
++
+AAAAAEEEEEEEEEEEEEEEEEEEEEAEAEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:23131:2747___1:N:0:TCCTGAGC+TACTCCTT;FP:61606;RQ:34.61;RQ:-1.00
+GAATAAGAGACAGATGAGCAGTATAGGGAGCATATTATTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACTAA
++
+A/AAAEEEEAEEAE<EEEEE/E6E///EE/EEE/6//6E/EEAAEEE/EAE<E//6AAAEEE/EE6AEE6EE//A
+@NB500968:8:H5CFGAFXX:1:11101:20373:2751___1:N:0:TCCTGATC+TACTCCTT;FP:283;RQ:34.93;RQ:-1.00
+GATGTGTATAAGAGACAGGATCACTTTATAGGCCN
++
+AAAAAEE6AAEE/E<EEEEAE/E6</A/EAEA/E#
+@NB500968:8:H5CFGAFXX:1:11101:21204:2795___1:N:0:TCCTGAGC+TACTCCTT;FP:16279;RQ:34.81;RQ:-1.00
+CAGTTAGGGCCTATAGGGTGGAGCNNNNNNNNNNN
++
+AAA<AEEEEEEEEEEEEEEEEEEE###########
+@NB500968:8:H5CFGAFXX:1:11101:17699:2880___1:N:0:TCCTGAGC+TACTCCTT;FP:52710;RQ:34.62;RQ:-1.00
+CATCTGACGCAGCCGACGAAAAAACATGTGTAGATCTCTGACTTCTCAGTATCATTAAATAAAAAAAATTGATGG
++
+AAAAAEEEEE/EEEEEEE6EEA//<<EEEE//E//EEEAE///E/6///E/EA/EA//////66A//<6A///AA
+@NB500968:8:H5CFGAFXX:1:11101:2261:2941___1:N:0:TCCTGAGC+TACTCCTT;FP:2596;RQ:34.63;RQ:-1.00
+CTCTTATACACATCTGATGCTGCCCCCTATATGCTCCTT
++
+A/AAAEAEE/E/AEEEEEEEEEEEEEE/E/A<EEEEE//
+@NB500968:8:H5CFGAFXX:1:11101:25690:2959___1:N:0:TCCTGCGC+TACTCCTT;FP:27004;RQ:34.81;RQ:-1.00
+GTGTATAAGAGACAGTCCGAAGCTATAGGGGCGGC
++
+A/AA/EEEEEE/6EEE//E//6EEEAAE/EE/EEE
+@NB500968:8:H5CFGAFXX:1:11101:8090:2972___1:N:0:TCCTGAGC+TACTCCTT;FP:12902;RQ:34.81;RQ:-1.00
+GGCATAAATTATAGGGGGTCCCNNNNNNNNNNNNN
++
+AAAAAEEEEEEEE6EAEEEEAE#############
+@NB500968:8:H5CFGAFXX:1:11101:16936:3211___1:N:0:TCCTGAGC+TACTCCTT;FP:7401;RQ:34.83;RQ:-1.00
+CAGTTCACTTTTATAGGGACGCACNNNNNNNNNNN
++
+AAAAAEEEEEEEEEEEEEEEEEEE###########
+@NB500968:8:H5CFGAFXX:1:11101:6132:3257___1:N:0:TCCTGAGC+TACTCCTT;FP:7557;RQ:34.62;RQ:-1.00
+GTATAAGAGACAGGAGGCGTGTATAGGGGAATGCN
++
+AAAAAEEEEEEEEEEEEEEEEEEEAEEEE6EEEE#
+@NB500968:8:H5CFGAFXX:1:11101:3840:3285___1:N:0:TCCTGAGC+TACTCCTT;FP:16156;RQ:34.93;RQ:-1.00
+CAATAGGGTATACCTGTCACTAATACACATCAGACGCTCCCCCCTATATAGTAAAT
++
+A/AAAE//EE/EEAE//E/EE/EEEEEEEEE/EEEAEEEE<A<A/</AE<EEAE<6
+@NB500968:8:H5CFGAFXX:1:11101:2858:3312___1:N:0:TCCTGAGC+TACTCCTT;FP:14452;RQ:35.12;RQ:-1.00
+CTCTTATACACATCTGACGTTCCCTATATGAGTCAT
++
+/AAAAEEAEEEEEEEEEEEEEEEEEAEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:3641:3398___1:N:0:TCCTGAGC+TACTCCTT;FP:36051;RQ:35.14;RQ:-1.00
+CTCTTATACACATCTGACGCTGCCCCTATAATGATATC
++
+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:20540:3401___1:N:0:TCCTGAGC+TACTCCTT;FP:48603;RQ:34.81;RQ:-1.00
+CAGTTAGGGCAAAAAGGGTTGATNNNNNNNNNNNN
++
+AAAA//EEAE6/E//AAA//EEE############
+@NB500968:8:H5CFGAFXX:1:11101:4772:3477___1:N:0:TCCTGAGC+AACTCCTT;FP:61788;RQ:3.33;RQ:-1.00
+GTTTATAATAGACACATAACAATAAAAGAGTAACAATCACAAAAAAAAATAAGACTCTCACGACGAAACACCTTA
++
+A//A/E//E/A/E/A/E//A<E/////E/E//AE////E/AE</////EEA/E/A/E//AE/E///<<///A<A/
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/paired_un_READ1.fq	Mon May 09 04:08:01 2016 -0400
@@ -0,0 +1,112 @@
+@NB500968:8:H5CFGAFXX:1:11101:15939:1071___1:N:0:TCCTGAGC+TACTCCTT;FP:23586;RQ:34.63;RQ:-1.00
+GGCACGTCAGATGAGTATAAGAGACAGCAAATAGGTATAGGGGGCACGTCAGATGAGTAA
++
+EAEEEEEEA///A/EAEEE/EAEEEEEEEEEEAEEE//E/AA/E6AE/EE/<E////A//
+@NB500968:8:H5CFGAFXX:1:11101:11473:1333___1:N:0:TCCTGAGC+TACTCCTT;FP:48246;RQ:35.03;RQ:-1.00
+GACAGCGTCAGATGTGTATAAGAGACAGGGTATC
++
+EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:2141:1369___1:N:0:TCCTGAGC+TACTCCTT;FP:0;RQ:1.56;RQ:-1.00
+NNNNNNNNNNNNNNNNNNNN
++
+####################
+@NB500968:8:H5CFGAFXX:1:11101:19474:1422___1:N:0:TCCTGAGC+TACTCCTT;FP:5233;RQ:35.03;RQ:-1.00
+GGAGCGTCAGATGTGTATAAGAGACAGGTACCC
++
+EEEEEEEEEEEEEEEEAEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:8662:1469___1:N:0:TCCTGAGC+TACTCCTT;FP:62135;RQ:35.03;RQ:-1.00
+GGAGCGTCAGATGTGTATAAGAGACAGGTTATC
++
+EEEEEEEEEEEEEAEAE<AEAEEEEEEEEEE<E
+@NB500968:8:H5CFGAFXX:1:11101:21998:1542___1:N:0:TCCTGAGC+TACTCCTT;FP:15180;RQ:35.09;RQ:-1.00
+GGCGTCAGATGTGTATAAGAGACAGGG
++
+EEEEEEEAEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:2814:1550___1:N:0:TCCTGAGC+TACTCCTT;FP:0;RQ:1.56;RQ:-1.00
+NNNNNNNNNNNNNNNNNNNN
++
+####################
+@NB500968:8:H5CFGAFXX:1:11101:8843:1628___1:N:0:TCCTGAGC+TACTCCTT;FP:44455;RQ:34.63;RQ:-1.00
+GGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGG
++
+EEEEEEEEEAEAEEEEEEEEEEE6/AEEEEAAEE6EA<EEEEE<EEEEEEEEE/AAAAEA
+@NB500968:8:H5CFGAFXX:1:11101:1482:1752___1:N:0:TCCTGAGC+TACTCCTT;FP:20529;RQ:35.03;RQ:-1.00
+GGGAGCGTCAGATGTGTATAAGAGACAGTTTTACCC
++
+E/EEEEEEEEEE</EEEEEEEEEEEEEEEEE<EAEA
+@NB500968:8:H5CFGAFXX:1:11101:12567:1818___1:N:0:TCCTGAGC+TACTCCTT;FP:61471;RQ:34.63;RQ:-1.00
+GAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGG
++
+EEEEE/EA/EEEAEEEEEA/EEAEEEEE/EEEA/EEAEAEE/A<E/EEEAA<<A6AA<//
+@NB500968:8:H5CFGAFXX:1:11101:26750:1960___1:N:0:TCCTGAGC+TACTCCTT;FP:578;RQ:35.03;RQ:-1.00
+GGGAGCGTCAGATGTGTATAAGAGACAGCTGACCC
++
+EEEAEEEAEEAEAEAEE/EEEEAEAAEEEEE/EEE
+@NB500968:8:H5CFGAFXX:1:11101:21600:1964___1:N:0:TCCTGAGC+TACTCCTT;FP:38123;RQ:18.05;RQ:-1.00
+GAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAA
++
+EEEEEEAEE/AAAEEEEEEEEEEEEEE<EEEAEEEEAAAEAAEEEE<EA<A<EA<<E<E
+@NB500968:8:H5CFGAFXX:1:11101:11625:2006___1:N:0:TCCTGAGC+TACTCCTT;FP:4030;RQ:35.03;RQ:-1.00
+GACAGCGTCAGATGTGTATAAGAGACAGCAACCACC
++
+AEA66E/<AEAEE/E/EEAEEEEAEEEEEAEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:10474:2017___1:N:0:TCCTGAGC+TACTCCTT;FP:16506;RQ:35.03;RQ:-1.00
+GGAGCGTCAGATGTGTATAAGAGACAGTCGTCC
++
+EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:23302:2031___1:N:0:TCCTGAGC+TACTCCTT;FP:9055;RQ:34.81;RQ:-1.00
+AGCGTCAGATGTGTATAAGAGACAGATCGTCCCTATAGCTCCAATC
++
+EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEAEEEEEEAEE
+@NB500968:8:H5CFGAFXX:1:11101:19822:2253___1:N:0:TCCTGAGC+TACTCCTT;FP:41486;RQ:34.63;RQ:-1.00
+GACGTGCAAATCGGTCGTCCGACCTGGGTATAGGGGCGAAAGACTAATCGAACCATCTAG
++
+EEEEEEEEEEEEEAEEEEEEEEEEEEAEEEEEAEAEE/EEEEEEEEE<<EEEEAEEEA//
+@NB500968:8:H5CFGAFXX:1:11101:5499:2506___1:N:0:TCCTGAGC+TACTCCTT;FP:0;RQ:1.56;RQ:-1.00
+NNNNNNNNNNNNNNNNNNNN
++
+####################
+@NB500968:8:H5CFGAFXX:1:11101:25015:2619___1:N:0:TCCTGAGC+TACTCCTT;FP:0;RQ:1.56;RQ:-1.00
+NNNNNNNNNNNNNNNNNNNN
++
+####################
+@NB500968:8:H5CFGAFXX:1:11101:25905:2738___1:N:0:TCCTGAGC+TACTCCTT;FP:0;RQ:1.56;RQ:-1.00
+NNNNNNNNNNNNNNNNNNNN
++
+####################
+@NB500968:8:H5CFGAFXX:1:11101:10694:2817___1:N:0:TCCTGAGC+TACTCCTT;FP:10036;RQ:34.83;RQ:-1.00
+GGAGCGTCAGATGTGTATAAGAGACAGCCCTTCCCTATACGCTTGGCC
++
+EEEEEEEEEEEEE<EEEEAEEEEEEEEEE/A<E<EEEA/EEEAEEEE/
+@NB500968:8:H5CFGAFXX:1:11101:8407:2853___1:N:0:TCCTGAGC+TACTCCTT;FP:21171;RQ:34.62;RQ:-1.00
+AGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTT
++
+/EEEEEEAEEEEEAEEEEEEEEEAEAEEAAEEEE/EEEAAAEA//EEEAEAAA/EAEAE6
+@NB500968:8:H5CFGAFXX:1:11101:11672:2944___1:N:0:TCCTGAGC+AACTCCTT;FP:32501;RQ:34.63;RQ:-1.00
+GGGTGAAATACTGTGAGGAACCCAGAAGTGAGTGGAAAGATAACTGGCTTTTTTTTTTTT
++
+AEEEEEEEEEEEAAAEEEEEAA<E/EEEEEEEAAEAEEEE<EEE<</EE6AA/6/<66//
+@NB500968:8:H5CFGAFXX:1:11101:15968:2945___1:N:0:TCCTGAGC+TACTCCTT;FP:8706;RQ:34.63;RQ:-1.00
+GCAAATCGGTCGTCCGACCTGGGTATAGGGGCGAAAGGCTAATCGAACCATCTAGTAG
++
+EEEEEEEAEEEEEEEEEEEEEEEEAEEEEEEAEEEEE<EEEEAAEAEEAE<AEAEEEE
+@NB500968:8:H5CFGAFXX:1:11101:22125:3141___1:N:0:TCCTGAGC+TACTCCTT;FP:65197;RQ:35.07;RQ:-1.00
+AGCGTCAGATGTGTATAAGAGACAGCTC
++
+EEEEEEEEEEEEEEEEEEEEEEEAEEEE
+@NB500968:8:H5CFGAFXX:1:11101:6707:3162___1:N:0:TCCTGAGC+TACTCCTT;FP:0;RQ:1.56;RQ:-1.00
+NNNNNNNNNNNNNNNNNNNN
++
+####################
+@NB500968:8:H5CFGAFXX:1:11101:24232:3202___1:N:0:TCCTGAGC+TACTCCTT;FP:19483;RQ:34.63;RQ:-1.00
+GTTGGTTTTCGGAACTGAGGCCATGATTAAGATGGACGGCCGGGGGCATTCGTATTGCG
++
+EEEEEEEEEEAEEEEEEE/E<EEEEEA</EEE/A/EE<E666<EEAEEEE/6/A66/66
+@NB500968:8:H5CFGAFXX:1:11101:6680:3212___1:N:0:TCCTGAGC+TACTCCTT;FP:62506;RQ:34.81;RQ:-1.00
+TCCAGCGTCAGATGTGTATAAGAGACAGCTCCTCCCTATAAG
++
+EEEEEEEEEEEEEEEEEEEAEEEEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:19438:3463___1:N:0:TCCTGAGC+TACTCCTT;FP:0;RQ:1.56;RQ:-1.00
+NNNNNNNNNNNNNNNNNNNN
++
+####################
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/paired_un_READ2.fq	Mon May 09 04:08:01 2016 -0400
@@ -0,0 +1,112 @@
+@NB500968:8:H5CFGAFXX:1:11101:15939:1071___1:N:0:TCCTGAGC+TACTCCTT;FP:23586;RQ:34.63;RQ:-1.00
+NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
++
+###################################
+@NB500968:8:H5CFGAFXX:1:11101:11473:1333___1:N:0:TCCTGAGC+TACTCCTT;FP:48246;RQ:35.03;RQ:-1.00
+NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
++
+###################################
+@NB500968:8:H5CFGAFXX:1:11101:2141:1369___1:N:0:TCCTGAGC+TACTCCTT;FP:0;RQ:1.56;RQ:-1.00
+GATGTGTATAAGAAACAGACCACATATATAGGGGGGAGCGTCAGATGTGTATAAGAGACAGCCCCCCCCAATAGA
++
+AAAAAEEEEEEEAEEEEE/E/EAEEEEEAEEEA/EEEEEEEAA//AEEEE/</AA/////////AAEE////AA/
+@NB500968:8:H5CFGAFXX:1:11101:19474:1422___1:N:0:TCCTGAGC+TACTCCTT;FP:5233;RQ:35.03;RQ:-1.00
+NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
++
+###################################
+@NB500968:8:H5CFGAFXX:1:11101:8662:1469___1:N:0:TCCTGAGC+TACTCCTT;FP:62135;RQ:35.03;RQ:-1.00
+NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
++
+###################################
+@NB500968:8:H5CFGAFXX:1:11101:21998:1542___1:N:0:TCCTGAGC+TACTCCTT;FP:15180;RQ:35.09;RQ:-1.00
+NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
++
+###################################
+@NB500968:8:H5CFGAFXX:1:11101:2814:1550___1:N:0:TCCTGAGC+TACTCCTT;FP:0;RQ:1.56;RQ:-1.00
+CCGTCAGATGAGTATAAGAGACAGGTAAGACATAAAGAGCGTAGTTGTCTCTAATACACATCTGACGCTGCCGA
++
+/AAA6AEEE//E/EAAEE6/EEEEEEEE//EE/E/EE/AE/<A//</AE/E////AE<A/<A/6/E<EEAAEEE
+@NB500968:8:H5CFGAFXX:1:11101:8843:1628___1:N:0:TCCTGAGC+TACTCCTT;FP:44455;RQ:34.63;RQ:-1.00
+GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAA
++
+AAAAAEEEEEEEEEEEEEEEEEAEEEEEEEEEEEEEEEEEAEEEEEEEEEE<EEEEEEEEEEEE<AA/E6EEAA6
+@NB500968:8:H5CFGAFXX:1:11101:1482:1752___1:N:0:TCCTGAGC+TACTCCTT;FP:20529;RQ:35.03;RQ:-1.00
+NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
++
+###################################
+@NB500968:8:H5CFGAFXX:1:11101:12567:1818___1:N:0:TCCTGAGC+TACTCCTT;FP:61471;RQ:34.63;RQ:-1.00
+GGTCATGGGAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGT
++
+AA/AAEEAEEE//AEAEEEEEEEEEEAEEEEEEEE/EAEE<EE<EA/<E<EEEEE6EEEE/EEEEEE/EEAEE
+@NB500968:8:H5CFGAFXX:1:11101:26750:1960___1:N:0:TCCTGAGC+TACTCCTT;FP:578;RQ:35.03;RQ:-1.00
+NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
++
+###################################
+@NB500968:8:H5CFGAFXX:1:11101:21600:1964___1:N:0:TCCTGAGC+TACTCCTT;FP:38123;RQ:18.05;RQ:-1.00
+GCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACG
++
+AAAAAEEEEEEAEEEEE/E/EEE6/EEEEEEEE/EEEEEEAEEEEEEAEEEAEEEEEEEEEAEE<EAEAEEE
+@NB500968:8:H5CFGAFXX:1:11101:11625:2006___1:N:0:TCCTGAGC+TACTCCTT;FP:4030;RQ:35.03;RQ:-1.00
+NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
++
+###################################
+@NB500968:8:H5CFGAFXX:1:11101:10474:2017___1:N:0:TCCTGAGC+TACTCCTT;FP:16506;RQ:35.03;RQ:-1.00
+NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
++
+###################################
+@NB500968:8:H5CFGAFXX:1:11101:23302:2031___1:N:0:TCCTGAGC+TACTCCTT;FP:9055;RQ:34.81;RQ:-1.00
+NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
++
+###################################
+@NB500968:8:H5CFGAFXX:1:11101:19822:2253___1:N:0:TCCTGAGC+TACTCCTT;FP:41486;RQ:34.63;RQ:-1.00
+GGCTTCTTACCCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCGGCCCCAAGACCTCTAATCATTCGCTTTA
++
+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEAEEEEEEEE<
+@NB500968:8:H5CFGAFXX:1:11101:5499:2506___1:N:0:TCCTGAGC+TACTCCTT;FP:0;RQ:1.56;RQ:-1.00
+GATTTGCCTTATAGGGAGCGTCAGATTTGTATAAGAGACAGCTCCACCCTATACTCGCCGC
++
+A//A<<AA<////A/E/E6<///A//////////////6///<//////////////////
+@NB500968:8:H5CFGAFXX:1:11101:25015:2619___1:N:0:TCCTGAGC+TACTCCTT;FP:0;RQ:1.56;RQ:-1.00
+GCGTCAGATGTGAGCGTCAGATGTGTATAAGAGACAGGATCCCCCTATAGCAAGAAG
++
+AAAA/AAA<//A/E6A6EEE//A/6//</A6E/AE6/EE<EAEEEA</AEE///AAE
+@NB500968:8:H5CFGAFXX:1:11101:25905:2738___1:N:0:TCCTGAGC+TACTCCTT;FP:0;RQ:1.56;RQ:-1.00
+GTCAGATGTGTATAAGAGACAGTGTGTGAGTATAGGGAAACC
++
+/AAAAEE/AEAA////AEAA/AAEE/EEEAE/E//AE/E/EA
+@NB500968:8:H5CFGAFXX:1:11101:10694:2817___1:N:0:TCCTGAGC+TACTCCTT;FP:10036;RQ:34.83;RQ:-1.00
+NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
++
+###################################
+@NB500968:8:H5CFGAFXX:1:11101:8407:2853___1:N:0:TCCTGAGC+TACTCCTT;FP:21171;RQ:34.62;RQ:-1.00
+CACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGGATGGGGTTCAACGGGT
++
+AAA6AEEEEEEE/EE/EEEEEEEEEEEEEEA6EEEEEEEE<EEEE</EEEAEEEEAEEEEEEEEAEEEE/EAEEA
+@NB500968:8:H5CFGAFXX:1:11101:11672:2944___1:N:0:TCCTGAGC+AACTCCTT;FP:32501;RQ:34.63;RQ:-1.00
+AAAACCCTAACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
++
+A/AA<//6/6//////66666666/6///AEE//EE/</AE</E/<EEEAEEEEEA////<EEE/E<E<A///<6
+@NB500968:8:H5CFGAFXX:1:11101:15968:2945___1:N:0:TCCTGAGC+TACTCCTT;FP:8706;RQ:34.63;RQ:-1.00
+GATGTGGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCCGCAGCGCCAGTTCTGCTTACCAAAAGT
++
+AAAA/EEEEEEEEEAEEEEEEE/EEEEAEEEEEEEEEEEEEAEEE<<EEA<AEE//AAA<EAA/EEEEEEEEEE<
+@NB500968:8:H5CFGAFXX:1:11101:22125:3141___1:N:0:TCCTGAGC+TACTCCTT;FP:65197;RQ:35.07;RQ:-1.00
+NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
++
+###################################
+@NB500968:8:H5CFGAFXX:1:11101:6707:3162___1:N:0:TCCTGAGC+TACTCCTT;FP:0;RQ:1.56;RQ:-1.00
+GCGTAAGAAAAATATAAAACACACTTAAACTCAATAAGTCCATAAATACTATTAAACACAAAAAAAGCATCCAAA
++
+///A/A/E</</EA<///A//<//E/EE//6EA/<//A/</6E//6//<///<//A6<<A/AA//////////</
+@NB500968:8:H5CFGAFXX:1:11101:24232:3202___1:N:0:TCCTGAGC+TACTCCTT;FP:19483;RQ:34.63;RQ:-1.00
+TGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAG
++
+AA/AAE/AA/E6/E/EEA<AAEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEA6AAEE/E/EEEEA<EAEE
+@NB500968:8:H5CFGAFXX:1:11101:6680:3212___1:N:0:TCCTGAGC+TACTCCTT;FP:62506;RQ:34.81;RQ:-1.00
+NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
++
+###################################
+@NB500968:8:H5CFGAFXX:1:11101:19438:3463___1:N:0:TCCTGAGC+TACTCCTT;FP:0;RQ:1.56;RQ:-1.00
+GCGTCGAGATGTGTATAAGAGACAGCCGAGCTCTATAGGGCAAGT
++
+AAAAAEEEEEEEEEEE<AEEEEEEEEEEEEEEEEEEEEEAEEEEE
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/read1.fastq	Mon May 09 04:08:01 2016 -0400
@@ -0,0 +1,400 @@
+@NB500968:8:H5CFGAFXX:1:11101:15939:1071 1:N:0:TCCTGAGC+TACTCCTT
+CCTAAGAGTATAGGGGGCACGTCAGATGAGTATAAGAGACAGCAAATAGGTATAGGGGGCACGTCAGATGAGTAA
++
+AAAAA6/AEEEEEEEEAEEEEEEA///A/EAEEE/EAEEEEEEEEEEAEEE//E/AA/E6AE/EE/<E////A//
+@NB500968:8:H5CFGAFXX:1:11101:1121:1139 1:N:0:TCGTGAGC+TACTCCTT
+CGTAGTCATATAGGGGGAGCGTCAGATGTGTATAAGAGACAGGTATTCCCTATAGTCTACATC
++
+AAAAAEEEEAEE<EEEEEEEEEEEEEEEEEAAEEEEAEEEAEEE/EEE/EE<AAEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:7529:1175 1:N:0:TCCTGAGC+TACTCCTT
+CAATTATGTATAGGGAGCGTCAGATGTGTATAAGAGACAGCAACCCTAAAAATATCCA
++
+AAAAAEA/EEEEEEEEEEE/EEEEEEA/EA<EEEE/AEEEE//E//6A/AE/A/AAE/
+@NB500968:8:H5CFGAFXX:1:11101:5052:1198 1:N:0:TCCTGAGC+TACTCCTT
+GCCTGATTTATAGGGGGTTTTTAGTGGCATTCATTGTGTGGATTCCTCTGTTGCTGCCCCTGGGCTAACAAGGGT
++
+AAAAAEEEEEEEE<AEEEEEEE6EEEEAEEEEEEEEEEEAEEEEEEEEEEEEEAAE/AEEEEEAAAEEEE/EAEE
+@NB500968:8:H5CFGAFXX:1:11101:15805:1205 1:N:0:TCCTGAGC+TACTCCTT
+GGTCTTTGTATAGGGGAGGGCCAGGCAGCTCAAAGCCTGAAGGAGGTACTTCAAGGAGGGTCCAGAGAAGCTGGG
++
+AAAAAEEEEEEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEE<EAAEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:11834:1217 1:N:0:TCCTGAGC+TACTCCTT
+TCTCTCGTTATAGGGAGCGTCAGATGTGTATAAGAGACAGGATTCCCCTATATAGCGACCC
++
+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEEEAEEEEEEEEEA<EAEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:20105:1230 1:N:0:TCCTGAGC+TACTCCTT
+AAGATCGCTATAGGGGTTGTTTCATGTTGCAATTTTTAGGTTACTTTTGAGTTGCTTAGGAATTGTTTTATTTAA
++
+6AAAA//EEEAEAEEEEEEEEEEEE/E/AEEEEE<E</EEAEEEEEEAAEAEEEAAE//EA//</E6EAAEA/6/
+@NB500968:8:H5CFGAFXX:1:11101:14524:1264 1:N:0:TCCTGAGC+TACTCCTT
+ACTTAAACTATAAGTCCCAACTTAATATTATAAAATTATCATAAAAACAACTACGAGCCCACGATACAACTGATA
++
+AAAAA//EEE<EEEA//EEEE<///A/AAA/A////E/<///E/6/E/E/E6///E//6E<//6/AA//E//A//
+@NB500968:8:H5CFGAFXX:1:11101:14981:1265 1:N:0:TCCTGAGC+TACTCCTT
+CGCGTCTCTATAGGGGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCG
++
+AAAAAEEEEEEE<EAEEEEEEEEEEEEEAEEEEEEEEEAAEEEEEEEA<EEEEEEEEE6/E66A<A/<AA<A//6
+@NB500968:8:H5CFGAFXX:1:11101:5538:1286 1:N:0:TCCTGAGC+TACTCCTT
+ACATTCCTTATAGGGAGTGAACTAACTAGAAAACTTCTAACTAAAAGAATTACAGCTAGAAACCCCGAAACCAAA
++
+6AAAAEEEEE<E</<AAEEEEEEEEAE/AEEEEEEEAEEEEE/AE6/EEA<E/EA6EAAEEE6//6<<EEA/<<A
+@NB500968:8:H5CFGAFXX:1:11101:11473:1333 1:N:0:TCCTGAGC+TACTCCTT
+GTTACTCGTATAGGGGACAGCGTCAGATGTGTATAAGAGACAGGGTATC
++
+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:2141:1369 1:N:0:TCCTGAGC+TACTCCTT
+NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
++
+###################################
+@NB500968:8:H5CFGAFXX:1:11101:24482:1380 1:N:0:TCCTGAGC+TACTCCTT
+GTGTATAAGAGACAGGCCTAATATATAGGGGGCGCCAGTCCTCCGATTGACTGAGTCGCCCGGGTACCCGTGTAT
++
+AAAAAEE/E/E/<EEEE/EAEE<EEEEEEEEEEEEEAEEA/E/EAEEEEEEEEEEEEEEE<E/EE<<6EEAEEEE
+@NB500968:8:H5CFGAFXX:1:11101:2671:1393 1:N:0:TCCTGAGC+TACTCCTT
+GGTCCCCTATAAGGGGATTCTGTCTCTTATACCNN
++
+///AA6E/6AA///EAE//E6E6E6E/6AAE/E##
+@NB500968:8:H5CFGAFXX:1:11101:19474:1422 1:N:0:TCCTGAGC+TACTCCTT
+ACCACTACTATAGGGGGAGCGTCAGATGTGTATAAGAGACAGGTACCC
++
+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:12411:1441 1:N:0:TCCTGAGC+TACTCCTT
+GTATTCCTTATAGGGGGGAGCGTCAGATGTGTATAAGAGACAGTCTCCCTATATTTTGGC
++
+AAAAA//AEEEEEEE//AEEE/EAEAEE/AEAE<EEEEAE/EAE<E/E</EEEE/6E/EE
+@NB500968:8:H5CFGAFXX:1:11101:5889:1458 1:N:0:TCCTGAGC+TACTCCTT
+TAGTAGAGTATAGGGGAGCGTCAGATGTGTATAAGAGACAGCGTATCCCTATACCCCTTCGCTG
++
+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:8662:1469 1:N:0:TCCTGAGC+TACTCCTT
+TTAGGTCTTATAGGGGGAGCGTCAGATGTGTATAAGAGACAGGTTATC
++
+AAAAAEEEEEEEEEEEEEEEEEEEEEEEAEAE<AEAEEEEEEEEEE<E
+@NB500968:8:H5CFGAFXX:1:11101:9851:1471 1:N:0:TCCTGAGC+TACTCCTT
+GGGCCCTATAACGGGCCCCTGTCTCTTATACCNNN
++
+AAAA6EEEEEEEAEEEEEEEA/AA/EEEEE//###
+@NB500968:8:H5CFGAFXX:1:11101:7850:1487 1:N:0:TCCTGAGC+TACTCCTT
+ATTCCAATATAGGGGACCCACATCTTCCTCTGCTTCTAGATGAAGGATGTATTCTTCTTCCTCTTCTTCCTCTG
++
+AAAAAEEE/EEE/EEEEEEAAEEE/EEEEEAEEEEEEEEEEEEEEAEAE6EEA<EEEAEE<6E/<EE<<E6E/<
+@NB500968:8:H5CFGAFXX:1:11101:5425:1488 1:N:0:TCCTGAGC+TACTCCTT
+ATCACATGTATAGGGGGAGCGTCAGATGTGTATAAGAGACAGACTCCCCTATATGGACACA
++
+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:21998:1542 1:N:0:TCCTGAGC+TACTCCTT
+ATGTCATATATAGGGGGCGTCAGATGTGTATAAGAGACAGGG
++
+AAAAA6EEEEEEEEEEEEEEEEAEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:2814:1550 1:N:0:TCCTGAGC+TACTCCTT
+NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
++
+###################################
+@NB500968:8:H5CFGAFXX:1:11101:8024:1581 1:N:0:TCCTGAGC+TACTCCTT
+GCCCGGCTTATAGGGGTGACCCGCCGCTTCAGGGACCCGCTTCTCTCATCTTCCCCAAAGTTCTGGACAGATGAT
++
+AAAAAEEEA/EE/A/EEEEEEEAEEEEEAEAEEEEEEEEEEAEEEEAEEEAEAEEEEEEEEAEEEEEEEEEE/A<
+@NB500968:8:H5CFGAFXX:1:11101:8843:1628 1:N:0:TCCTGAGC+TACTCCTT
+GGTCGGCTTATAGGGGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGG
++
+AA/AAEEEEEEE<E/EEEEEEEEEAEAEEEEEEEEEEE6/AEEEEAAEE6EA<EEEEE<EEEEEEEEE/AAAAEA
+@NB500968:8:H5CFGAFXX:1:11101:23474:1673 1:N:0:TCCTGAGC+TACTCCTT
+AAGTGCGATATAGGGAGCGTCAGATGTGTATAAGAGACAGAGGCGCCCTATAGAATATAT
++
+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEE/EEEEE
+@NB500968:8:H5CFGAFXX:1:11101:13010:1719 1:N:0:TCCTGAGC+TACTCCTT
+AAGACAGTGTGATTATATAGGGGACAGCGTCAGATGTGTATAAGAGACAGCTAAGCCCTATACGTTTTTTC
++
+/A/AAEAAEEEE/E/EAEAAEE</EEEEEEAEEEEAAEAAAEAEEEAEEEEE/EE/EEE6A/EEEAEEEEA
+@NB500968:8:H5CFGAFXX:1:11101:15935:1725 1:N:0:GCCTGAGC+TACTCCTT
+GTGTATAAGAAACAGGCTTTATATAGGGGCACCGTCAGATGTGTATAAGAGACAGTTGTCCCCTATACCACGC
++
+/AAAAEEEEAEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE/E<<EEEEE/EE<EEEE/AEEEEEEEAEEE
+@NB500968:8:H5CFGAFXX:1:11101:1482:1752 1:N:0:TCCTGAGC+TACTCCTT
+CCAAATACTATAGGGGGGAGCGTCAGATGTGTATAAGAGACAGTTTTACCC
++
+AAAAAEEEAA/AEEEE/EEEEEEEEEE</EEEEEEEEEEEEEEEEE<EAEA
+@NB500968:8:H5CFGAFXX:1:11101:21720:1786 1:N:0:TCCTGAGC+TACTCCTT
+ATTTGAACTATAGGGGGGAGCGTCAGATGTGTATAAGAGAC
++
+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:9012:1809 1:N:0:TCCTGAGC+TACTCCTT
+ACAACACTTATAGGGGGGAGCGTCAGATGTGTATAAGAGACAGGTACCCCCTATATCATAGGC
++
+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:12567:1818 1:N:0:TCCTGAGC+TACTCCTT
+TTAAACTTTATAGGGGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGG
++
+AAAAAEEEEEEE<E/EEEEE/EA/EEEAEEEEEA/EEAEEEEE/EEEA/EEAEAEE/A<E/EEEAA<<A6AA<//
+@NB500968:8:H5CFGAFXX:1:11101:20110:1819 1:N:0:TCCTGAGC+TAATCCTT
+TTTAAGTTTATAGGGGGATTCTTGAAGTATAACAGGAGAATCAAATAATTAAAGAGGGGGCTGGAGAGATAGCT
++
+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:24842:1887 1:N:0:TCCTGAGC+TACTCCTT
+ATCCTATATATAGGGGGGAGCGTCAGATGTGTATAAGAG
++
+AAAAAEEAEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:12108:1890 1:N:0:TCCTGAGC+TACTCCTT
+CAGTTAGGTGTTATAGGGATCCCAAGACAAGGTTTCTCTGTGTCTATCCCTGGCTGTCCTTGAACTTCGGCAT
++
+6AAAA66EAE6EEEEEAE/EAAEEE/EEEEAEEEEEE/EEEEE/EAEEE/E/EAEEE<<EE/EAEEE6/EEE<
+@NB500968:8:H5CFGAFXX:1:11101:16709:1904 1:N:0:TCCTGAGC+TACTCCTT
+ACCGAGCTTATAGGGGTTCCATGCGTCAATGTTTCTTCTGGATACAGCAGGCCTGTTCCCGGGAGTCAATGC
++
+/AAAAEEEEEEEEEAEEEEEAEEEEEEEEEEAEEEEEEEEEEEAEE6<EEEEEAAA<EAEEEEEEEEEEE<E
+@NB500968:8:H5CFGAFXX:1:11101:12189:1919 1:N:0:TCCTGAGC+TACTCCTT
+TATTTCCATATAGGGACACCCTTTATAGTGATGGATTGGATCTGTTTGAAGGCAAAGTCCTTCCCAGACTTATCA
++
+AAAAA/EEEA6<///EEEEEEE/EEE/EEAEEEEEEEEEEE/EE<EAAAE/E<</EA///A</E/A/AAA<A/</
+@NB500968:8:H5CFGAFXX:1:11101:26750:1960 1:N:0:TCCTGAGC+TACTCCTT
+AAAGCAAGTATAGGGGGGAGCGTCAGATGTGTATAAGAGACAGCTGACCC
++
+AAAAAAEEE/<AEEAEEEAEEEAEEAEAEAEE/EEEEAEAAEEEEE/EEE
+@NB500968:8:H5CFGAFXX:1:11101:21600:1964 1:N:0:TCCTGAGC+TACTCCTT
+GCCATGGTTATAAGGGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAA
++
+AAAAAEEAEEEE/E/EEEEEEAEE/AAAEEEEEEEEEEEEEE<EEEAEEEEAAAEAAEEEE<EA<A<EA<<E<E
+@NB500968:8:H5CFGAFXX:1:11101:6961:1984 1:N:0:TCCTGAGC+TACTCCTT
+GGATGTCGCTATAGGGCGCCGCCGCCTCCTCCTCCTCTCTCGCGCTCTCTGTCCCGCCTGGTCCTGTCCCACCC
++
+AAAAAAEEEEEEEEEEEEEAEAAEEE</EEEE/EEEEEEAEEEAAEEEEEEEEEEEEEEAEEEAEEEEAE/EEE
+@NB500968:8:H5CFGAFXX:1:11101:20646:1998 1:N:0:TCCTGAGC+TACTCCTT
+CCGCAAGTGCGTTCGAAGTGTCGATGATCAATGTGTCCTGCAATTCACATTAATTCTCGCAGCTAGCTGCGTT
++
+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEAEEEEEEAEEEEEEEEEEEEEEEEAAEE
+@NB500968:8:H5CFGAFXX:1:11101:11625:2006 1:N:0:TCCTGAGC+TACTCCTT
+AATTGTTGTATAGGGGACAGCGTCAGATGTGTATAAGAGACAGCAACCACC
++
+AAAA/AAA/EEEEEEAEA66E/<AEAEE/E/EEAEEEEAEEEEEAEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:10474:2017 1:N:0:TCCTGAGC+TACTCCTT
+CAAACTGGTATAGGGGGAGCGTCAGATGTGTATAAGAGACAGTCGTCC
++
+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:17381:2018 1:N:0:TCCTGAGC+TACTCCTT
+TTTATTAGTATAGGGAACGTCAGATGTGTATAAGN
++
+AAAAA/EAEEEE<EEEEE/EEEAEEEEEAE</EA#
+@NB500968:8:H5CFGAFXX:1:11101:9520:2027 1:N:0:TCCTGAGC+TACTCCTT
+TCTTCACTTATAGGGAGCGTCAGATGTGTATAAGAGACAGCTACATCCCTATAAGGATGTAC
++
+AAAAAEEEEEEEEEAEEEEEEEEEEEEEEEEAEEEEEEEEEEAEEEEEEE/EEEEE<AEEEA
+@NB500968:8:H5CFGAFXX:1:11101:23302:2031 1:N:0:TCCTGAGC+TACTCCTT
+AGATCCTTTATAGGGAGCGTCAGATGTGTATAAGAGACAGATCGTCCCTATAGCTCCAATC
++
+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEAEEEEEEAEE
+@NB500968:8:H5CFGAFXX:1:11101:19677:2089 1:N:0:TCCTGAGC+TACTCCTT
+ATGCGTGATATAGGGGGCGCCAGTCCTCCGATTGACTGAGTCGCCCGGGTACCCGTGTATCCAATAAACCCTCTT
++
+/AAAAEAEEEEE<EEEEEEEEEAEEEEEEEAE/EEEEEEEEAEEE//E/EEE/A<AAAAAA<AEAE/EE/AAA<6
+@NB500968:8:H5CFGAFXX:1:11101:26158:2095 1:N:0:TCCTGAGC+TACTCCTT
+CCTCATTATATAGGGGGGAGCGTCAGATGTGTATAAGAGACAGCCACTCCCTATAGGATTTCC
++
+AAAAAAEEEEEEEEEEEEEEEEEEAEEEEEEEEEEEEEEEEEEA<EEEEAEEA<6EEEEE<EA
+@NB500968:8:H5CFGAFXX:1:11101:7883:2101 1:N:0:TCCGGAGC+TACTCCTT
+TGGACGTCTATAGGGGGAGCGTCAGATGTGTATAAGAGACAGCATCACCCTATAGGCTGTCT
++
+AAA6AEEEEE/EEEEEEEEEEEEEEEEEEAEAEEEEEEEEEEEEEEEEEEEEE<EEEEEEAE
+@NB500968:8:H5CFGAFXX:1:11101:19922:2125 1:N:0:TCCTGAGC+TACTCCTT
+GTATAAGAGACAGCGATACTTTATAGGGGGGCCGTGTGCAGACCCGCGTGTGGCGCAGGCAAGGACCCTGAAAAT
++
+AAAAAEE/EAEEEEEEEEEEEEEEEE/EEEEEEEEAEEEEEEEEEEEEAEEA<<EEEEEEEEEEEEEEEEEEEA<
+@NB500968:8:H5CFGAFXX:1:11101:24496:2131 1:N:0:TCCTGAGC+TACTCCTT
+AAAATTCTTATAGGGGGGAGCGTCAGATGTGTATAAG
++
+/AAAAEEEEAE/EEAEEEEEEEEEEE/EEEEEEE/AE
+@NB500968:8:H5CFGAFXX:1:11101:5814:2162 1:N:0:TCCTGAGC+TACTCCTT
+ACCGCTCTTATAGGGGCCCTTGGCTTCACCACACTTTCTACTGGAAATGTGCCTTTTCCTCCATTGCTGCTAC
++
+/AAAAEEEA/EE/E/EEEEEEEEEEEEEEEAAEEEAEEEEEA/EEEEEE<EEEAEAAEEAEEE<AAEAEEAAE
+@NB500968:8:H5CFGAFXX:1:11101:5796:2184 1:N:0:TCCTGAGC+TATTCCTT
+ACAAGCGGTATAGGGGGAGCGTCAGATGTGTATAAGAGACAGGCTGTCCCTATAGACTGACT
++
+AAAAAEEEEEAEAEEEEEEEEEEEEEEEEEAEAEEEEEEEEEEEEEEEEAEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:23937:2223 1:N:0:TCCTGAGC+TACTCCTT
+CGCCGGCGTATAGGGGCTTCTTCTGAAGAGCTCCACCTGTGGTCTCTCCACCTCTCGAGGCTCTTCACTAGCCT
++
+6AAAAAEEAE6EEA/EEEEAEA/AEEEEEEEA/</</</A//EAEEE<AE66A666/A/E6E/AAAAA////E/
+@NB500968:8:H5CFGAFXX:1:11101:25267:2246 1:N:0:TCCTGAGC+TACTCCTT
+ACAACATCTATAGGGGAGCGTCAGATGTGTATAAGAGACAGGATGCCCCTATATATAGAGGCTG
++
+AAAAAAEEEEAEAEEE6EEEEAAEEEEEEEAEEEEEEEEEEEEEEE6EEEEEEEEEAEAEEAEA
+@NB500968:8:H5CFGAFXX:1:11101:19822:2253 1:N:0:TCCTGAGC+TACTCCTT
+GGAGAATGTATAGGGGACGTGCAAATCGGTCGTCCGACCTGGGTATAGGGGCGAAAGACTAATCGAACCATCTAG
++
+AA6AAEEEEEEEEAEEEEEEEEEEEEEEAEEEEEEEEEEEEAEEEEEAEAEE/EEEEEEEEE<<EEEEAEEEA//
+@NB500968:8:H5CFGAFXX:1:11101:6670:2262 1:N:0:TCCTGAGC+TACTCCTT
+ATGATAGTTATGGGTGGAGGCCAAATTGTGCTGATTTTCCTGTAGCTGCGATTAATAGGCCTATAAGTGGAATTA
++
+/AAAAEEEEAAE<EEEEEEEEEEEEAEAEEAEEEEEEEE/EAEEEAEEAEEAE/EEAE/</E/EA66A<//<EE/
+@NB500968:8:H5CFGAFXX:1:11101:14935:2297 1:N:0:TCCTGAGC+TACTCCTT
+GATGTGCATAAAAGACAGATAGTACGTATAGGGGGGAGCGTCAGATGTTTATAAGAGACAGAATCGCCCTATAG
++
+AAAAAAAEEAEEEEEEEEEEEEEEEEEEEEEEEAEEEEEAE/A/EEAE/E/6/E///E/E/</E///A/AA/</
+@NB500968:8:H5CFGAFXX:1:11101:14858:2304 1:N:0:TCCTGAGC+TACTCCTT
+ACAACGGGTATAGGGGTCTCCGGCTTGTGTAGACTCTGCCTGGAAGTGAAGTTGTGGACTCCCGACCTCCAGTC
++
+AAAAAEEEEEEEEE/EEEEEEEEEEEEEEEEEEEEEA/E/AEEEEEAEEEEAE/<AAEEE<EAEEE<EEAEEEE
+@NB500968:8:H5CFGAFXX:1:11101:10463:2345 1:N:0:GCCTGAGC+TACTCCTT
+CCTTACACTATAGGGTCCAGCGTCAGATGTGTATAAGAGACAGAGTTCCCCTATAGAGAGACG
++
+AAAAAEEEEEEEEEEEEEEEEEEEEE6EEEEA/A//E/EEEEEEEEEEEEEEAA/AAEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:24405:2351 1:N:0:TCCTGAGC+TACTCCTT
+ACCCTATATATAGGGTCTGTCTCTTATACCNNNNN
++
+6AAAAEEEAEEEEEEEEEEEEAEEEEEEEE#####
+@NB500968:8:H5CFGAFXX:1:11101:6508:2370 1:N:0:TCCTGAGC+TACTCCTT
+TTGTGGCGTATAGGGGGAGCGTCAGATGTGTATAAGAGACAGCCCAGCCCTATACCGTCTAGCTG
++
+AAAAAEEEEEEEEEEEEEEEEEEEEEEEAEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:13250:2472 1:N:0:TCCTGAGC+TACTCCTT
+GTCTCCGCCCTTGCTGACTTTCCATCATTTTTAAGATCAGGTGGAACTGGAGCCTCTGCCTGTGGCTGATCTGAG
++
+AAAAAEAA/EAA<EEEEE/EEAAEEAE<EEA/E//EE/E/EEAAEEEEA6A/6EAA6/6<6/<//A/<AAE6AA/
+@NB500968:8:H5CFGAFXX:1:11101:21730:2488 1:N:0:TCGTGAGC+TACTCCTT
+TCTTTACATATAGGGAGCGTCAGATGTGTATCNNN
++
+AAAAAEAEEE<EEEEEEEEEEEEEEEEEAEAE###
+@NB500968:8:H5CFGAFXX:1:11101:5499:2506 1:N:0:TCCTGAGC+TACTCCTT
+NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
++
+###################################
+@NB500968:8:H5CFGAFXX:1:11101:20021:2516 1:N:0:TCCTGGGC+TACTCCTT
+CGTGGTTTTATAGGGGGAGCGTCAGATGAGTATAAGAGACAGTCATCCCCTATAGTCGTTCA
++
+AAA66E/EEEEAEEEEAEEAAEEAAE///E/EAAE/EAEE//EEE/EEEEEE/EE/EA6EEE
+@NB500968:8:H5CFGAFXX:1:11101:4827:2579 1:N:0:TCCTGAGC+TACTCCTT
+AGACGTAGTATAGGGGACAGCGTCAGATGTGTATAAGAGACAGGGAATCCCTATAGACTTTGC
++
+AAAAAEEE6EAEEEEEEEEEEEEE/E<AAAEEEEEEEE/E/EEEEEEEEEEEAEEAEAAEEE/
+@NB500968:8:H5CFGAFXX:1:11101:25015:2619 1:N:0:TCCTGAGC+TACTCCTT
+NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
++
+###################################
+@NB500968:8:H5CFGAFXX:1:11101:11758:2662 1:N:0:TCCTGAGC+TACTCCTT
+CTTGATCATATAGGGGACAGCGTCAGATGTGTATAAGAGACAGAGATGCCCTATAGACCGAAC
++
+AAAAAAEEEEEEE/EEEEEEE/EAE/EEE/EEEAEE6E6EEEEEAAEA/E/AEEEA/E6EA/E
+@NB500968:8:H5CFGAFXX:1:11101:25905:2738 1:N:0:TCCTGAGC+TACTCCTT
+NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
++
+###################################
+@NB500968:8:H5CFGAFXX:1:11101:23131:2747 1:N:0:TCCTGAGC+TACTCCTT
+TTAAGGCGTATAGGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCGAGGTTATCTA
++
+AAAAAEAEEEEEE<AEEEEEEAEAEEEE/EEEAEEEEA/EEEEAEA/EA<EAAAEEAE<AE/A<<EEEA<<EEA<
+@NB500968:8:H5CFGAFXX:1:11101:20373:2751 1:N:0:TCCTGATC+TACTCCTT
+AAACACGTTATAGGGGGAGCGTCAGATGTGTATAAGAGACAGCCCTATACAGTGATC
++
+AAAAAEEEEEEEEEEEEEEEEEEE<EEEEAEAEEEEEEEEEEEEEE/EEEEEE<EAA
+@NB500968:8:H5CFGAFXX:1:11101:8485:2792 1:N:0:TCCTGAGC+TACTCCTT
+CGTCAGATGTGGTCCCGCTCACCATGCCGCTGCTGTAGCCGCTCACCAACCTCCTGTGCCCCCCGCAACTCAT
++
+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEE6EEEEEEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:21204:2795 1:N:0:TCCTGAGC+TACTCCTT
+ATTTGCCTTATAGGGAGCGTCAGATGTGTATAAGAGACAGCTCCACCCTATAGGCCCTAACTG
++
+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:10694:2817 1:N:0:TCCTGAGC+TACTCCTT
+AGCTATCATATAGGGGGAGCGTCAGATGTGTATAAGAGACAGCCCTTCCCTATACGCTTGGCC
++
+AAAAAEAEEEEEEEEEEEEEEEEEEEEE<EEEEAEEEEEEEEEE/A<E<EEEA/EEEAEEEE/
+@NB500968:8:H5CFGAFXX:1:11101:8407:2853 1:N:0:TCCTGAGC+TACTCCTT
+CCAGGTATTATAGGGAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTT
++
+6AAAAEAEE/AE//</EEEEEEAEEEEEAEEEEEEEEEAEAEEAAEEEE/EEEAAAEA//EEEAEAAA/EAEAE6
+@NB500968:8:H5CFGAFXX:1:11101:17699:2880 1:N:0:TCCTGAGC+TACTCCTT
+TATCTGCGTATAGGGCTTTGGAATCTTCACAAGCAGAACCAGTCAAAACCCCGGCAAGCACAAAGAGACTCTCCA
++
+AAAAAEEEAEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE<EEEEEEEEEEE6AEAEAEEEEA
+@NB500968:8:H5CFGAFXX:1:11101:2261:2941 1:N:0:TCCTGAGC+TACTCCTT
+AAGGAGCATATAGGGGGCAGCATCAGATGTGTATAAGAGACAGAAGGAGCAGAGAGCGGCCAGCAACCGATGAGA
++
+AAAAAEEEEEAEEEEEEEEEEEEEEEEEEAEEEAEEEEE///////EA//E/E/A/////E<EE//</E<<////
+@NB500968:8:H5CFGAFXX:1:11101:11672:2944 1:N:0:TCCTGAGC+AACTCCTT
+CTTGTTCCTATAGGGGGGTGAAATACTGTGAGGAACCCAGAAGTGAGTGGAAAGATAACTGGCTTTTTTTTTTTT
++
+AAAAAEAEEEEEE<AAEEEEEEEEEEEAAAEEEEEAA<E/EEEEEEEAAEAEEEE<EEE<</EE6AA/6/<66//
+@NB500968:8:H5CFGAFXX:1:11101:15968:2945 1:N:0:TCCTGAGC+TACTCCTT
+AGAGAAAGTATAGGGGCAAATCGGTCGTCCGACCTGGGTATAGGGGCGAAAGGCTAATCGAACCATCTAGTAG
++
+AAAAAEEEEEEEEEEEEEEEEEAEEEEEEEEEEEEEEEEAEEEEEEAEEEEE<EEEEAAEAEEAE<AEAEEEE
+@NB500968:8:H5CFGAFXX:1:11101:25690:2959 1:N:0:TCCTGCGC+TACTCCTT
+CGGCCTTATATAGGGAGCGTCAGATGTGTATAAGAGACAGGCCTCCCCTATAGCTTCGGA
++
+AAAA6EEE//6EEA/AE6EEEEEEEEEAAEEEEEEEEEEEEEEAEE/E/EAEEEEAEEEE
+@NB500968:8:H5CFGAFXX:1:11101:8090:2972 1:N:0:TCCTGAGC+TACTCCTT
+ATAGCGCGTATAGGGCAACGTCAGATGTGTATAAGAGACAGGGACCCCCTATAATTTATGCC
++
+AAAAAEEEEEAEEEEEEEEEEEEEEEEEEEEEAAEEEEEEEEEAEE<EEEEEAE<EEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:23310:3069 1:N:0:TCCTGAGC+TACTCCTT
+CAGGCCAATCGTATAGGGGTTGATACTCAAGTCTACTTGTTCCCTTTTATTTAATCCTGAACCCCATCCCATGGA
++
+AAA/AEEEEEEEAE//EEE////EAA//EE/EEEEEE//EEE/E/E/EEEEEEE<A<E/AEE//AE/<AAEEEE/
+@NB500968:8:H5CFGAFXX:1:11101:9254:3110 1:N:0:TCCTGAGC+TACTCCTT
+TGTTATAAGGCGTCAGATGTGTATAAGAGACAGGGATCCCCTATAAAGAGCCTAAC
++
+AAAAAEEEEEEEEEEEEEEEEEEEAEEEE<EAEEE/EEEEEEEEE/EEEEEEEAA/
+@NB500968:8:H5CFGAFXX:1:11101:22125:3141 1:N:0:TCCTGAGC+TACTCCTT
+TTTGGGTCTATAGGGAGCGTCAGATGTGTATAAGAGACAGCTC
++
+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEE
+@NB500968:8:H5CFGAFXX:1:11101:6707:3162 1:N:0:TCCTGAGC+TACTCCTT
+NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
++
+###################################
+@NB500968:8:H5CFGAFXX:1:11101:4099:3187 1:N:0:TCCTGAGC+TACTCCTT
+GTATAAGAGACAGCCAGCCTCTATAGGGGTGCTTGCAGGGGAGGGAAAAGGGGGAAAGCGGGCAACCAGTTTCC
++
+AAAAAAEAE6E6EEEEEAEEA<EEEEEEEEEEEEEEEEEEEEEEEEEEAEE/EEAEEEEEEEEEEEEEAE/E//
+@NB500968:8:H5CFGAFXX:1:11101:24232:3202 1:N:0:TCCTGAGC+TACTCCTT
+CATAACGTTATAGGGGTTGGTTTTCGGAACTGAGGCCATGATTAAGATGGACGGCCGGGGGCATTCGTATTGCG
++
+AAAAAEEEEEAE///EEEEEEEEEEAEEEEEEE/E<EEEEEA</EEE/A/EE<E666<EEAEEEE/6/A66/66
+@NB500968:8:H5CFGAFXX:1:11101:16936:3211 1:N:0:TCCTGAGC+TACTCCTT
+ACTATGGCTATAGGGGAGCGTCAGATGTGTATAAGAGACAGTGCGTCCCTATAAAAGTGAACTG
++
+AAAAAEEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEEEEEEA/EEEAEAEEE
+@NB500968:8:H5CFGAFXX:1:11101:6680:3212 1:N:0:TCCTGAGC+TACTCCTT
+TTCAAGGGTATAGGGTCCAGCGTCAGATGTGTATAAGAGACAGCTCCTCCCTATAAG
++
+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:15733:3232 1:N:0:TCCTGAGC+TACTCCTT
+TCCCCTATATTTGGGGACTGTCTCTTATACACCNN
++
+AAAAAEEE/EEEEEEEEEEEEEEEEEEEEEEEE##
+@NB500968:8:H5CFGAFXX:1:11101:3009:3242 1:N:0:TCCTGAGC+TACTCCTT
+GGTTAAGGCTAGCCTATTCTACATAGAGTGTTCTAGGCCTTCCAGGGCCACATAGACTCTTGTTTCAAAAAAAT
++
+A//AAEEEEEEEEEEEAEE/EEEEAAEE/AAEEEAEEEEAEEEEAEEEEEEEEEEEEEEA6EEEE<EEE/A/EA
+@NB500968:8:H5CFGAFXX:1:11101:6132:3257 1:N:0:TCCTGAGC+TACTCCTT
+ACTCGACCTATAGGGCGTCAGATGTGTATAAGAGACAGCATTCCCCTATACACGCCTCCTGTCTCTTATAC
++
+AAAAA/EEE6E/EEEEEEEAEEEEEEEEEEEEEEAEAEEAEEEEAEEEEEEEEEEEEEEEEAE/EEEEEEA
+@NB500968:8:H5CFGAFXX:1:11101:3840:3285 1:N:0:TCCTGAGC+TACTCCTT
+ATTTACTATATAGGGGGGAGCGTCAGATGTGTATAAGAGACAGGTATACCCTATAG
++
+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:2858:3312 1:N:0:TCCTGAGC+TACTCCTT
+ATGACTCATATAGGGAACGTCAGATGTGTATAAGAG
++
+AAAAAE/EE/A/<AEEE/AE/E/EAAEEEEEEEAEE
+@NB500968:8:H5CFGAFXX:1:11101:3641:3398 1:N:0:TCCTGAGC+TACTCCTT
+GATATCATTATAGGGGCAGCGTCAGATGTGTATAAGAG
++
+AAAAAEEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:20540:3401 1:N:0:TCCTGAGC+TACTCCTT
+GTTCTCGTTATAGGGAGCGTCAGATGTGTATAAGAGACAGATCAACCCTATATGCCCTAACTG
++
+AAA6AE/AAEEEEEEE/E///E/E</A/EEA<E/E/EEE/E</E///A/E6<E/A/<E/EEE/
+@NB500968:8:H5CFGAFXX:1:11101:18503:3442 1:N:0:TCCTGAGC+TACTCCGT
+TAATCCCTATAAGGGATTACTGACTCTTATACATGTATCTAATACACATCTCCGAGCCCACGAGACTCCTGAGCA
++
+AAAAAE/AAEEEEEEAAE/E//////EEAA///A/E/<///AEEEE/E/EE<<///E<EEE/E/AE6/EEEE//6
+@NB500968:8:H5CFGAFXX:1:11101:19438:3463 1:N:0:TCCTGAGC+TACTCCTT
+NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
++
+###################################
+@NB500968:8:H5CFGAFXX:1:11101:4772:3477 1:N:0:TCCTGAGC+AACTCCTT
+TTACCCTATACTGGGTAACTGTCTCTTATACACCN
++
+AAAAAEEEEAEEEEAEEEEEEEAEEEAEEE<EEE#
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/read1.fq	Mon May 09 04:08:01 2016 -0400
@@ -0,0 +1,328 @@
+@NB500968:8:H5CFGAFXX:1:11101:15939:1071 1:N:0:TCCTGAGC+TACTCCTT;FP:23586;RQ:34.63
+GGCACGTCAGATGAGTATAAGAGACAGCAAATAGGTATAGGGGGCACGTCAGATGAGTAA
++
+EAEEEEEEA///A/EAEEE/EAEEEEEEEEEEAEEE//E/AA/E6AE/EE/<E////A//
+@NB500968:8:H5CFGAFXX:1:11101:1121:1139 1:N:0:TCGTGAGC+TACTCCTT;FP:27828;RQ:34.83
+GGAGCGTCAGATGTGTATAAGAGACAGGTATTCCCTATAGTCTACATC
++
+EEEEEEEEEEEEEEEAAEEEEAEEEAEEE/EEE/EE<AAEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:7529:1175 1:N:0:TCCTGAGC+TACTCCTT;FP:17358;RQ:34.81
+AGCGTCAGATGTGTATAAGAGACAGCAACCCTAAAAATATCCA
++
+EEEE/EEEEEEA/EA<EEEE/AEEEE//E//6A/AE/A/AAE/
+@NB500968:8:H5CFGAFXX:1:11101:5052:1198 1:N:0:TCCTGAGC+TACTCCTT;FP:38799;RQ:34.63
+GGTTTTTAGTGGCATTCATTGTGTGGATTCCTCTGTTGCTGCCCCTGGGCTAACAAGGGT
++
+EEEEEEE6EEEEAEEEEEEEEEEEAEEEEEEEEEEEEEAAE/AEEEEEAAAEEEE/EAEE
+@NB500968:8:H5CFGAFXX:1:11101:15805:1205 1:N:0:TCCTGAGC+TACTCCTT;FP:44542;RQ:34.63
+GAGGGCCAGGCAGCTCAAAGCCTGAAGGAGGTACTTCAAGGAGGGTCCAGAGAAGCTGGG
++
+EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEE<EAAEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:11834:1217 1:N:0:TCCTGAGC+TACTCCTT;FP:56795;RQ:34.81
+AGCGTCAGATGTGTATAAGAGACAGGATTCCCCTATATAGCGACCC
++
+EEEEEEEEEEEEEEEEAEEEEEEEEEEAEEEEEEEEEA<EAEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:20105:1230 1:N:0:TCCTGAGC+TACTCCTT;FP:2265;RQ:34.63
+GTTGTTTCATGTTGCAATTTTTAGGTTACTTTTGAGTTGCTTAGGAATTGTTTTATTTAA
++
+EEEEEEEEEE/E/AEEEEE<E</EEAEEEEEEAAEAEEEAAE//EA//</E6EAAEA/6/
+@NB500968:8:H5CFGAFXX:1:11101:14524:1264 1:N:0:TCCTGAGC+TACTCCTT;FP:7937;RQ:2.65
+CCCAACTTAATATTATAAAATTATCATAAAAACAACTACGAGCCCACGATACAACTGATA
++
+//EEEE<///A/AAA/A////E/<///E/6/E/E/E6///E//6E<//6/AA//E//A//
+@NB500968:8:H5CFGAFXX:1:11101:14981:1265 1:N:0:TCCTGAGC+TACTCCTT;FP:26333;RQ:34.63
+GTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCG
++
+EEEEEEEEEEEEEAEEEEEEEEEAAEEEEEEEA<EEEEEEEEE6/E66A<A/<AA<A//6
+@NB500968:8:H5CFGAFXX:1:11101:5538:1286 1:N:0:TCCTGAGC+TACTCCTT;FP:5079;RQ:34.62
+AGTGAACTAACTAGAAAACTTCTAACTAAAAGAATTACAGCTAGAAACCCCGAAACCAAA
++
+AAEEEEEEEEAE/AEEEEEEEAEEEEE/AE6/EEA<E/EA6EAAEEE6//6<<EEA/<<A
+@NB500968:8:H5CFGAFXX:1:11101:11473:1333 1:N:0:TCCTGAGC+TACTCCTT;FP:48246;RQ:35.03
+GACAGCGTCAGATGTGTATAAGAGACAGGGTATC
++
+EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:2141:1369 1:N:0:TCCTGAGC+TACTCCTT;FP:0;RQ:1.56
+NNNNNNNNNNNNNNNNNNNN
++
+####################
+@NB500968:8:H5CFGAFXX:1:11101:19474:1422 1:N:0:TCCTGAGC+TACTCCTT;FP:5233;RQ:35.03
+GGAGCGTCAGATGTGTATAAGAGACAGGTACCC
++
+EEEEEEEEEEEEEEEEAEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:12411:1441 1:N:0:TCCTGAGC+TACTCCTT;FP:46039;RQ:34.83
+GGGAGCGTCAGATGTGTATAAGAGACAGTCTCCCTATATTTTGGC
++
+//AEEE/EAEAEE/AEAE<EEEEAE/EAE<E/E</EEEE/6E/EE
+@NB500968:8:H5CFGAFXX:1:11101:5889:1458 1:N:0:TCCTGAGC+TACTCCTT;FP:52002;RQ:34.83
+GAGCGTCAGATGTGTATAAGAGACAGCGTATCCCTATACCCCTTCGCTG
++
+EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:8662:1469 1:N:0:TCCTGAGC+TACTCCTT;FP:62135;RQ:35.03
+GGAGCGTCAGATGTGTATAAGAGACAGGTTATC
++
+EEEEEEEEEEEEEAEAE<AEAEEEEEEEEEE<E
+@NB500968:8:H5CFGAFXX:1:11101:9851:1471 1:N:0:TCCTGAGC+TACTCCTT;FP:43356;RQ:3.17
+CCCCTGTCTCTTATACCNNN
++
+EEEEEA/AA/EEEEE//###
+@NB500968:8:H5CFGAFXX:1:11101:5425:1488 1:N:0:TCCTGAGC+TACTCCTT;FP:13390;RQ:34.83
+GGAGCGTCAGATGTGTATAAGAGACAGACTCCCCTATATGGACACA
++
+EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:21998:1542 1:N:0:TCCTGAGC+TACTCCTT;FP:15180;RQ:35.09
+GGCGTCAGATGTGTATAAGAGACAGGG
++
+EEEEEEEAEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:2814:1550 1:N:0:TCCTGAGC+TACTCCTT;FP:0;RQ:1.56
+NNNNNNNNNNNNNNNNNNNN
++
+####################
+@NB500968:8:H5CFGAFXX:1:11101:8024:1581 1:N:0:TCCTGAGC+TACTCCTT;FP:38311;RQ:34.63
+GTGACCCGCCGCTTCAGGGACCCGCTTCTCTCATCTTCCCCAAAGTTCTGGACAGATGAT
++
+EEEEEEEAEEEEEAEAEEEEEEEEEEAEEEEAEEEAEAEEEEEEEEAEEEEEEEEEE/A<
+@NB500968:8:H5CFGAFXX:1:11101:8843:1628 1:N:0:TCCTGAGC+TACTCCTT;FP:44455;RQ:34.63
+GGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGG
++
+EEEEEEEEEAEAEEEEEEEEEEE6/AEEEEAAEE6EA<EEEEE<EEEEEEEEE/AAAAEA
+@NB500968:8:H5CFGAFXX:1:11101:23474:1673 1:N:0:TCCTGAGC+TACTCCTT;FP:2968;RQ:34.81
+AGCGTCAGATGTGTATAAGAGACAGAGGCGCCCTATAGAATATAT
++
+EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEE/EEEEE
+@NB500968:8:H5CFGAFXX:1:11101:1482:1752 1:N:0:TCCTGAGC+TACTCCTT;FP:20529;RQ:35.03
+GGGAGCGTCAGATGTGTATAAGAGACAGTTTTACCC
++
+E/EEEEEEEEEE</EEEEEEEEEEEEEEEEE<EAEA
+@NB500968:8:H5CFGAFXX:1:11101:21720:1786 1:N:0:TCCTGAGC+TACTCCTT;FP:16257;RQ:35.14
+GGGAGCGTCAGATGTGTATAAGAGAC
++
+EEEEEEEEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:9012:1809 1:N:0:TCCTGAGC+TACTCCTT;FP:4167;RQ:34.83
+GGGAGCGTCAGATGTGTATAAGAGACAGGTACCCCCTATATCATAGGC
++
+EEEEEEEEEEEEEEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:12567:1818 1:N:0:TCCTGAGC+TACTCCTT;FP:61471;RQ:34.63
+GAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGG
++
+EEEEE/EA/EEEAEEEEEA/EEAEEEEE/EEEA/EEAEAEE/A<E/EEEAA<<A6AA<//
+@NB500968:8:H5CFGAFXX:1:11101:20110:1819 1:N:0:TCCTGAGC+TAATCCTT;FP:64559;RQ:34.63
+GGATTCTTGAAGTATAACAGGAGAATCAAATAATTAAAGAGGGGGCTGGAGAGATAGCT
++
+EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:24842:1887 1:N:0:TCCTGAGC+TACTCCTT;FP:13772;RQ:35.14
+GGGAGCGTCAGATGTGTATAAGAG
++
+EEEEEEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:16709:1904 1:N:0:TCCTGAGC+TACTCCTT;FP:5671;RQ:34.73
+GTTCCATGCGTCAATGTTTCTTCTGGATACAGCAGGCCTGTTCCCGGGAGTCAATGC
++
+EEEEEAEEEEEEEEEEAEEEEEEEEEEEAEE6<EEEEEAAA<EAEEEEEEEEEEE<E
+@NB500968:8:H5CFGAFXX:1:11101:12189:1919 1:N:0:TCCTGAGC+TACTCCTT;FP:53204;RQ:34.62
+ACACCCTTTATAGTGATGGATTGGATCTGTTTGAAGGCAAAGTCCTTCCCAGACTTATCA
++
+EEEEEEE/EEE/EEAEEEEEEEEEEE/EE<EAAAE/E<</EA///A</E/A/AAA<A/</
+@NB500968:8:H5CFGAFXX:1:11101:26750:1960 1:N:0:TCCTGAGC+TACTCCTT;FP:578;RQ:35.03
+GGGAGCGTCAGATGTGTATAAGAGACAGCTGACCC
++
+EEEAEEEAEEAEAEAEE/EEEEAEAAEEEEE/EEE
+@NB500968:8:H5CFGAFXX:1:11101:21600:1964 1:N:0:TCCTGAGC+TACTCCTT;FP:38123;RQ:18.05
+GAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAA
++
+EEEEEEAEE/AAAEEEEEEEEEEEEEE<EEEAEEEEAAAEAAEEEE<EA<A<EA<<E<E
+@NB500968:8:H5CFGAFXX:1:11101:11625:2006 1:N:0:TCCTGAGC+TACTCCTT;FP:4030;RQ:35.03
+GACAGCGTCAGATGTGTATAAGAGACAGCAACCACC
++
+AEA66E/<AEAEE/E/EEAEEEEAEEEEEAEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:10474:2017 1:N:0:TCCTGAGC+TACTCCTT;FP:16506;RQ:35.03
+GGAGCGTCAGATGTGTATAAGAGACAGTCGTCC
++
+EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:17381:2018 1:N:0:TCCTGAGC+TACTCCTT;FP:64754;RQ:35.18
+AACGTCAGATGTGTATAAGN
++
+EEE/EEEAEEEEEAE</EA#
+@NB500968:8:H5CFGAFXX:1:11101:9520:2027 1:N:0:TCCTGAGC+TACTCCTT;FP:57159;RQ:34.81
+AGCGTCAGATGTGTATAAGAGACAGCTACATCCCTATAAGGATGTAC
++
+EEEEEEEEEEEEEEEEAEEEEEEEEEEAEEEEEEE/EEEEE<AEEEA
+@NB500968:8:H5CFGAFXX:1:11101:23302:2031 1:N:0:TCCTGAGC+TACTCCTT;FP:9055;RQ:34.81
+AGCGTCAGATGTGTATAAGAGACAGATCGTCCCTATAGCTCCAATC
++
+EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEAEEEEEEAEE
+@NB500968:8:H5CFGAFXX:1:11101:19677:2089 1:N:0:TCCTGAGC+TACTCCTT;FP:14776;RQ:34.63
+GGCGCCAGTCCTCCGATTGACTGAGTCGCCCGGGTACCCGTGTATCCAATAAACCCTCTT
++
+EEEEEEEAEEEEEEEAE/EEEEEEEEAEEE//E/EEE/A<AAAAAA<AEAE/EE/AAA<6
+@NB500968:8:H5CFGAFXX:1:11101:26158:2095 1:N:0:TCCTGAGC+TACTCCTT;FP:23868;RQ:34.83
+GGGAGCGTCAGATGTGTATAAGAGACAGCCACTCCCTATAGGATTTCC
++
+EEEEEEEEEAEEEEEEEEEEEEEEEEEEA<EEEEAEEA<6EEEEE<EA
+@NB500968:8:H5CFGAFXX:1:11101:7883:2101 1:N:0:TCCGGAGC+TACTCCTT;FP:59501;RQ:34.83
+GGAGCGTCAGATGTGTATAAGAGACAGCATCACCCTATAGGCTGTCT
++
+EEEEEEEEEEEEEEAEAEEEEEEEEEEEEEEEEEEEEE<EEEEEEAE
+@NB500968:8:H5CFGAFXX:1:11101:24496:2131 1:N:0:TCCTGAGC+TACTCCTT;FP:247;RQ:35.19
+GGGAGCGTCAGATGTGTATAAG
++
+EEEEEEEEEEE/EEEEEEE/AE
+@NB500968:8:H5CFGAFXX:1:11101:5814:2162 1:N:0:TCCTGAGC+TACTCCTT;FP:5751;RQ:34.63
+GCCCTTGGCTTCACCACACTTTCTACTGGAAATGTGCCTTTTCCTCCATTGCTGCTAC
++
+EEEEEEEEEEEEEEEAAEEEAEEEEEA/EEEEEE<EEEAEAAEEAEEE<AAEAEEAAE
+@NB500968:8:H5CFGAFXX:1:11101:5796:2184 1:N:0:TCCTGAGC+TATTCCTT;FP:4250;RQ:34.83
+GGAGCGTCAGATGTGTATAAGAGACAGGCTGTCCCTATAGACTGACT
++
+EEEEEEEEEEEEEEEAEAEEEEEEEEEEEEEEEEAEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:23937:2223 1:N:0:TCCTGAGC+TACTCCTT;FP:26022;RQ:34.63
+GCTTCTTCTGAAGAGCTCCACCTGTGGTCTCTCCACCTCTCGAGGCTCTTCACTAGCCT
++
+EEEEAEA/AEEEEEEEA/</</</A//EAEEE<AE66A666/A/E6E/AAAAA////E/
+@NB500968:8:H5CFGAFXX:1:11101:25267:2246 1:N:0:TCCTGAGC+TACTCCTT;FP:4173;RQ:34.83
+GAGCGTCAGATGTGTATAAGAGACAGGATGCCCCTATATATAGAGGCTG
++
+E6EEEEAAEEEEEEEAEEEEEEEEEEEEEEE6EEEEEEEEEAEAEEAEA
+@NB500968:8:H5CFGAFXX:1:11101:19822:2253 1:N:0:TCCTGAGC+TACTCCTT;FP:41486;RQ:34.63
+GACGTGCAAATCGGTCGTCCGACCTGGGTATAGGGGCGAAAGACTAATCGAACCATCTAG
++
+EEEEEEEEEEEEEAEEEEEEEEEEEEAEEEEEAEAEE/EEEEEEEEE<<EEEEAEEEA//
+@NB500968:8:H5CFGAFXX:1:11101:6670:2262 1:N:0:TCCTGAGC+TACTCCTT;FP:14539;RQ:3.28
+GGAGGCCAAATTGTGCTGATTTTCCTGTAGCTGCGATTAATAGGCCTATAAGTGGAATTA
++
+EEEEEEEEEEAEAEEAEEEEEEEE/EAEEEAEEAEEAE/EEAE/</E/EA66A<//<EE/
+@NB500968:8:H5CFGAFXX:1:11101:14858:2304 1:N:0:TCCTGAGC+TACTCCTT;FP:4202;RQ:34.63
+GTCTCCGGCTTGTGTAGACTCTGCCTGGAAGTGAAGTTGTGGACTCCCGACCTCCAGTC
++
+EEEEEEEEEEEEEEEEEEEEEA/E/AEEEEEAEEEEAE/<AAEEE<EAEEE<EEAEEEE
+@NB500968:8:H5CFGAFXX:1:11101:10463:2345 1:N:0:GCCTGAGC+TACTCCTT;FP:24337;RQ:34.81
+TCCAGCGTCAGATGTGTATAAGAGACAGAGTTCCCCTATAGAGAGACG
++
+EEEEEEEEEEE6EEEEA/A//E/EEEEEEEEEEEEEEAA/AAEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:24405:2351 1:N:0:TCCTGAGC+TACTCCTT;FP:5580;RQ:35.18
+TCTGTCTCTTATACCNNNNN
++
+EEEEEEAEEEEEEEE#####
+@NB500968:8:H5CFGAFXX:1:11101:6508:2370 1:N:0:TCCTGAGC+TACTCCTT;FP:64422;RQ:34.83
+GGAGCGTCAGATGTGTATAAGAGACAGCCCAGCCCTATACCGTCTAGCTG
++
+EEEEEEEEEEEEEAEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:21730:2488 1:N:0:TCGTGAGC+TACTCCTT;FP:57284;RQ:35.18
+AGCGTCAGATGTGTATCNNN
++
+EEEEEEEEEEEEEAEAE###
+@NB500968:8:H5CFGAFXX:1:11101:5499:2506 1:N:0:TCCTGAGC+TACTCCTT;FP:0;RQ:1.56
+NNNNNNNNNNNNNNNNNNNN
++
+####################
+@NB500968:8:H5CFGAFXX:1:11101:20021:2516 1:N:0:TCCTGGGC+TACTCCTT;FP:28351;RQ:34.83
+GGAGCGTCAGATGAGTATAAGAGACAGTCATCCCCTATAGTCGTTCA
++
+EAEEAAEEAAE///E/EAAE/EAEE//EEE/EEEEEE/EE/EA6EEE
+@NB500968:8:H5CFGAFXX:1:11101:4827:2579 1:N:0:TCCTGAGC+TACTCCTT;FP:8626;RQ:34.83
+GACAGCGTCAGATGTGTATAAGAGACAGGGAATCCCTATAGACTTTGC
++
+EEEEEEEEE/E<AAAEEEEEEEE/E/EEEEEEEEEEEAEEAEAAEEE/
+@NB500968:8:H5CFGAFXX:1:11101:25015:2619 1:N:0:TCCTGAGC+TACTCCTT;FP:0;RQ:1.56
+NNNNNNNNNNNNNNNNNNNN
++
+####################
+@NB500968:8:H5CFGAFXX:1:11101:11758:2662 1:N:0:TCCTGAGC+TACTCCTT;FP:32308;RQ:34.83
+GACAGCGTCAGATGTGTATAAGAGACAGAGATGCCCTATAGACCGAAC
++
+EEEEEE/EAE/EEE/EEEAEE6E6EEEEEAAEA/E/AEEEA/E6EA/E
+@NB500968:8:H5CFGAFXX:1:11101:25905:2738 1:N:0:TCCTGAGC+TACTCCTT;FP:0;RQ:1.56
+NNNNNNNNNNNNNNNNNNNN
++
+####################
+@NB500968:8:H5CFGAFXX:1:11101:23131:2747 1:N:0:TCCTGAGC+TACTCCTT;FP:61606;RQ:34.61
+CAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCGAGGTTATCTA
++
+EEEEEEAEAEEEE/EEEAEEEEA/EEEEAEA/EA<EAAAEEAE<AE/A<<EEEA<<EEA<
+@NB500968:8:H5CFGAFXX:1:11101:20373:2751 1:N:0:TCCTGATC+TACTCCTT;FP:283;RQ:34.93
+GGAGCGTCAGATGTGTATAAGAGACAGCCCTATACAGTGATC
++
+EEEEEEEEE<EEEEAEAEEEEEEEEEEEEEE/EEEEEE<EAA
+@NB500968:8:H5CFGAFXX:1:11101:21204:2795 1:N:0:TCCTGAGC+TACTCCTT;FP:16279;RQ:34.81
+AGCGTCAGATGTGTATAAGAGACAGCTCCACCCTATAGGCCCTAACTG
++
+EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:10694:2817 1:N:0:TCCTGAGC+TACTCCTT;FP:10036;RQ:34.83
+GGAGCGTCAGATGTGTATAAGAGACAGCCCTTCCCTATACGCTTGGCC
++
+EEEEEEEEEEEEE<EEEEAEEEEEEEEEE/A<E<EEEA/EEEAEEEE/
+@NB500968:8:H5CFGAFXX:1:11101:8407:2853 1:N:0:TCCTGAGC+TACTCCTT;FP:21171;RQ:34.62
+AGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTT
++
+/EEEEEEAEEEEEAEEEEEEEEEAEAEEAAEEEE/EEEAAAEA//EEEAEAAA/EAEAE6
+@NB500968:8:H5CFGAFXX:1:11101:17699:2880 1:N:0:TCCTGAGC+TACTCCTT;FP:52710;RQ:34.62
+CTTTGGAATCTTCACAAGCAGAACCAGTCAAAACCCCGGCAAGCACAAAGAGACTCTCCA
++
+EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE<EEEEEEEEEEE6AEAEAEEEEA
+@NB500968:8:H5CFGAFXX:1:11101:2261:2941 1:N:0:TCCTGAGC+TACTCCTT;FP:2596;RQ:34.63
+GGCAGCATCAGATGTGTATAAGAGACAGAAGGAGCAGAGAGCGGCCAGCAACCGATGAGA
++
+EEEEEEEEEEEEEEAEEEAEEEEE///////EA//E/E/A/////E<EE//</E<<////
+@NB500968:8:H5CFGAFXX:1:11101:11672:2944 1:N:0:TCCTGAGC+AACTCCTT;FP:32501;RQ:34.63
+GGGTGAAATACTGTGAGGAACCCAGAAGTGAGTGGAAAGATAACTGGCTTTTTTTTTTTT
++
+AEEEEEEEEEEEAAAEEEEEAA<E/EEEEEEEAAEAEEEE<EEE<</EE6AA/6/<66//
+@NB500968:8:H5CFGAFXX:1:11101:15968:2945 1:N:0:TCCTGAGC+TACTCCTT;FP:8706;RQ:34.63
+GCAAATCGGTCGTCCGACCTGGGTATAGGGGCGAAAGGCTAATCGAACCATCTAGTAG
++
+EEEEEEEAEEEEEEEEEEEEEEEEAEEEEEEAEEEEE<EEEEAAEAEEAE<AEAEEEE
+@NB500968:8:H5CFGAFXX:1:11101:25690:2959 1:N:0:TCCTGCGC+TACTCCTT;FP:27004;RQ:34.81
+AGCGTCAGATGTGTATAAGAGACAGGCCTCCCCTATAGCTTCGGA
++
+AE6EEEEEEEEEAAEEEEEEEEEEEEEEAEE/E/EAEEEEAEEEE
+@NB500968:8:H5CFGAFXX:1:11101:8090:2972 1:N:0:TCCTGAGC+TACTCCTT;FP:12902;RQ:34.81
+CAACGTCAGATGTGTATAAGAGACAGGGACCCCCTATAATTTATGCC
++
+EEEEEEEEEEEEEEEEEAAEEEEEEEEEAEE<EEEEEAE<EEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:22125:3141 1:N:0:TCCTGAGC+TACTCCTT;FP:65197;RQ:35.07
+AGCGTCAGATGTGTATAAGAGACAGCTC
++
+EEEEEEEEEEEEEEEEEEEEEEEAEEEE
+@NB500968:8:H5CFGAFXX:1:11101:6707:3162 1:N:0:TCCTGAGC+TACTCCTT;FP:0;RQ:1.56
+NNNNNNNNNNNNNNNNNNNN
++
+####################
+@NB500968:8:H5CFGAFXX:1:11101:24232:3202 1:N:0:TCCTGAGC+TACTCCTT;FP:19483;RQ:34.63
+GTTGGTTTTCGGAACTGAGGCCATGATTAAGATGGACGGCCGGGGGCATTCGTATTGCG
++
+EEEEEEEEEEAEEEEEEE/E<EEEEEA</EEE/A/EE<E666<EEAEEEE/6/A66/66
+@NB500968:8:H5CFGAFXX:1:11101:16936:3211 1:N:0:TCCTGAGC+TACTCCTT;FP:7401;RQ:34.83
+GAGCGTCAGATGTGTATAAGAGACAGTGCGTCCCTATAAAAGTGAACTG
++
+EEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEEEEEEA/EEEAEAEEE
+@NB500968:8:H5CFGAFXX:1:11101:6680:3212 1:N:0:TCCTGAGC+TACTCCTT;FP:62506;RQ:34.81
+TCCAGCGTCAGATGTGTATAAGAGACAGCTCCTCCCTATAAG
++
+EEEEEEEEEEEEEEEEEEEAEEEEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:6132:3257 1:N:0:TCCTGAGC+TACTCCTT;FP:7557;RQ:34.62
+CGTCAGATGTGTATAAGAGACAGCATTCCCCTATACACGCCTCCTGTCTCTTATAC
++
+EEEEAEEEEEEEEEEEEEEAEAEEAEEEEAEEEEEEEEEEEEEEEEAE/EEEEEEA
+@NB500968:8:H5CFGAFXX:1:11101:3840:3285 1:N:0:TCCTGAGC+TACTCCTT;FP:16156;RQ:34.93
+GGGAGCGTCAGATGTGTATAAGAGACAGGTATACCCTATAG
++
+EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:2858:3312 1:N:0:TCCTGAGC+TACTCCTT;FP:14452;RQ:35.12
+AACGTCAGATGTGTATAAGAG
++
+EE/AE/E/EAAEEEEEEEAEE
+@NB500968:8:H5CFGAFXX:1:11101:3641:3398 1:N:0:TCCTGAGC+TACTCCTT;FP:36051;RQ:35.14
+GCAGCGTCAGATGTGTATAAGAG
++
+EEEEEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:20540:3401 1:N:0:TCCTGAGC+TACTCCTT;FP:48603;RQ:34.81
+AGCGTCAGATGTGTATAAGAGACAGATCAACCCTATATGCCCTAACTG
++
+E/E///E/E</A/EEA<E/E/EEE/E</E///A/E6<E/A/<E/EEE/
+@NB500968:8:H5CFGAFXX:1:11101:19438:3463 1:N:0:TCCTGAGC+TACTCCTT;FP:0;RQ:1.56
+NNNNNNNNNNNNNNNNNNNN
++
+####################
+@NB500968:8:H5CFGAFXX:1:11101:4772:3477 1:N:0:TCCTGAGC+AACTCCTT;FP:61788;RQ:3.33
+TAACTGTCTCTTATACACCN
++
+EEEEEEEAEEEAEEE<EEE#
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/read1_extracted.fastq	Mon May 09 04:08:01 2016 -0400
@@ -0,0 +1,328 @@
+@NB500968:8:H5CFGAFXX:1:11101:15939:1071___1:N:0:TCCTGAGC+TACTCCTT;FP:23586;RQ:34.63
+GGCACGTCAGATGAGTATAAGAGACAGCAAATAGGTATAGGGGGCACGTCAGATGAGTAA
++
+EAEEEEEEA///A/EAEEE/EAEEEEEEEEEEAEEE//E/AA/E6AE/EE/<E////A//
+@NB500968:8:H5CFGAFXX:1:11101:1121:1139___1:N:0:TCGTGAGC+TACTCCTT;FP:27828;RQ:34.83
+GGAGCGTCAGATGTGTATAAGAGACAGGTATTCCCTATAGTCTACATC
++
+EEEEEEEEEEEEEEEAAEEEEAEEEAEEE/EEE/EE<AAEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:7529:1175___1:N:0:TCCTGAGC+TACTCCTT;FP:17358;RQ:34.81
+AGCGTCAGATGTGTATAAGAGACAGCAACCCTAAAAATATCCA
++
+EEEE/EEEEEEA/EA<EEEE/AEEEE//E//6A/AE/A/AAE/
+@NB500968:8:H5CFGAFXX:1:11101:5052:1198___1:N:0:TCCTGAGC+TACTCCTT;FP:38799;RQ:34.63
+GGTTTTTAGTGGCATTCATTGTGTGGATTCCTCTGTTGCTGCCCCTGGGCTAACAAGGGT
++
+EEEEEEE6EEEEAEEEEEEEEEEEAEEEEEEEEEEEEEAAE/AEEEEEAAAEEEE/EAEE
+@NB500968:8:H5CFGAFXX:1:11101:15805:1205___1:N:0:TCCTGAGC+TACTCCTT;FP:44542;RQ:34.63
+GAGGGCCAGGCAGCTCAAAGCCTGAAGGAGGTACTTCAAGGAGGGTCCAGAGAAGCTGGG
++
+EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEE<EAAEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:11834:1217___1:N:0:TCCTGAGC+TACTCCTT;FP:56795;RQ:34.81
+AGCGTCAGATGTGTATAAGAGACAGGATTCCCCTATATAGCGACCC
++
+EEEEEEEEEEEEEEEEAEEEEEEEEEEAEEEEEEEEEA<EAEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:20105:1230___1:N:0:TCCTGAGC+TACTCCTT;FP:2265;RQ:34.63
+GTTGTTTCATGTTGCAATTTTTAGGTTACTTTTGAGTTGCTTAGGAATTGTTTTATTTAA
++
+EEEEEEEEEE/E/AEEEEE<E</EEAEEEEEEAAEAEEEAAE//EA//</E6EAAEA/6/
+@NB500968:8:H5CFGAFXX:1:11101:14524:1264___1:N:0:TCCTGAGC+TACTCCTT;FP:7937;RQ:2.65
+CCCAACTTAATATTATAAAATTATCATAAAAACAACTACGAGCCCACGATACAACTGATA
++
+//EEEE<///A/AAA/A////E/<///E/6/E/E/E6///E//6E<//6/AA//E//A//
+@NB500968:8:H5CFGAFXX:1:11101:14981:1265___1:N:0:TCCTGAGC+TACTCCTT;FP:26333;RQ:34.63
+GTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCG
++
+EEEEEEEEEEEEEAEEEEEEEEEAAEEEEEEEA<EEEEEEEEE6/E66A<A/<AA<A//6
+@NB500968:8:H5CFGAFXX:1:11101:5538:1286___1:N:0:TCCTGAGC+TACTCCTT;FP:5079;RQ:34.62
+AGTGAACTAACTAGAAAACTTCTAACTAAAAGAATTACAGCTAGAAACCCCGAAACCAAA
++
+AAEEEEEEEEAE/AEEEEEEEAEEEEE/AE6/EEA<E/EA6EAAEEE6//6<<EEA/<<A
+@NB500968:8:H5CFGAFXX:1:11101:11473:1333___1:N:0:TCCTGAGC+TACTCCTT;FP:48246;RQ:35.03
+GACAGCGTCAGATGTGTATAAGAGACAGGGTATC
++
+EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:2141:1369___1:N:0:TCCTGAGC+TACTCCTT;FP:0;RQ:1.56
+NNNNNNNNNNNNNNNNNNNN
++
+####################
+@NB500968:8:H5CFGAFXX:1:11101:19474:1422___1:N:0:TCCTGAGC+TACTCCTT;FP:5233;RQ:35.03
+GGAGCGTCAGATGTGTATAAGAGACAGGTACCC
++
+EEEEEEEEEEEEEEEEAEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:12411:1441___1:N:0:TCCTGAGC+TACTCCTT;FP:46039;RQ:34.83
+GGGAGCGTCAGATGTGTATAAGAGACAGTCTCCCTATATTTTGGC
++
+//AEEE/EAEAEE/AEAE<EEEEAE/EAE<E/E</EEEE/6E/EE
+@NB500968:8:H5CFGAFXX:1:11101:5889:1458___1:N:0:TCCTGAGC+TACTCCTT;FP:52002;RQ:34.83
+GAGCGTCAGATGTGTATAAGAGACAGCGTATCCCTATACCCCTTCGCTG
++
+EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:8662:1469___1:N:0:TCCTGAGC+TACTCCTT;FP:62135;RQ:35.03
+GGAGCGTCAGATGTGTATAAGAGACAGGTTATC
++
+EEEEEEEEEEEEEAEAE<AEAEEEEEEEEEE<E
+@NB500968:8:H5CFGAFXX:1:11101:9851:1471___1:N:0:TCCTGAGC+TACTCCTT;FP:43356;RQ:3.17
+CCCCTGTCTCTTATACCNNN
++
+EEEEEA/AA/EEEEE//###
+@NB500968:8:H5CFGAFXX:1:11101:5425:1488___1:N:0:TCCTGAGC+TACTCCTT;FP:13390;RQ:34.83
+GGAGCGTCAGATGTGTATAAGAGACAGACTCCCCTATATGGACACA
++
+EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:21998:1542___1:N:0:TCCTGAGC+TACTCCTT;FP:15180;RQ:35.09
+GGCGTCAGATGTGTATAAGAGACAGGG
++
+EEEEEEEAEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:2814:1550___1:N:0:TCCTGAGC+TACTCCTT;FP:0;RQ:1.56
+NNNNNNNNNNNNNNNNNNNN
++
+####################
+@NB500968:8:H5CFGAFXX:1:11101:8024:1581___1:N:0:TCCTGAGC+TACTCCTT;FP:38311;RQ:34.63
+GTGACCCGCCGCTTCAGGGACCCGCTTCTCTCATCTTCCCCAAAGTTCTGGACAGATGAT
++
+EEEEEEEAEEEEEAEAEEEEEEEEEEAEEEEAEEEAEAEEEEEEEEAEEEEEEEEEE/A<
+@NB500968:8:H5CFGAFXX:1:11101:8843:1628___1:N:0:TCCTGAGC+TACTCCTT;FP:44455;RQ:34.63
+GGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGG
++
+EEEEEEEEEAEAEEEEEEEEEEE6/AEEEEAAEE6EA<EEEEE<EEEEEEEEE/AAAAEA
+@NB500968:8:H5CFGAFXX:1:11101:23474:1673___1:N:0:TCCTGAGC+TACTCCTT;FP:2968;RQ:34.81
+AGCGTCAGATGTGTATAAGAGACAGAGGCGCCCTATAGAATATAT
++
+EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEE/EEEEE
+@NB500968:8:H5CFGAFXX:1:11101:1482:1752___1:N:0:TCCTGAGC+TACTCCTT;FP:20529;RQ:35.03
+GGGAGCGTCAGATGTGTATAAGAGACAGTTTTACCC
++
+E/EEEEEEEEEE</EEEEEEEEEEEEEEEEE<EAEA
+@NB500968:8:H5CFGAFXX:1:11101:21720:1786___1:N:0:TCCTGAGC+TACTCCTT;FP:16257;RQ:35.14
+GGGAGCGTCAGATGTGTATAAGAGAC
++
+EEEEEEEEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:9012:1809___1:N:0:TCCTGAGC+TACTCCTT;FP:4167;RQ:34.83
+GGGAGCGTCAGATGTGTATAAGAGACAGGTACCCCCTATATCATAGGC
++
+EEEEEEEEEEEEEEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:12567:1818___1:N:0:TCCTGAGC+TACTCCTT;FP:61471;RQ:34.63
+GAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGG
++
+EEEEE/EA/EEEAEEEEEA/EEAEEEEE/EEEA/EEAEAEE/A<E/EEEAA<<A6AA<//
+@NB500968:8:H5CFGAFXX:1:11101:20110:1819___1:N:0:TCCTGAGC+TAATCCTT;FP:64559;RQ:34.63
+GGATTCTTGAAGTATAACAGGAGAATCAAATAATTAAAGAGGGGGCTGGAGAGATAGCT
++
+EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:24842:1887___1:N:0:TCCTGAGC+TACTCCTT;FP:13772;RQ:35.14
+GGGAGCGTCAGATGTGTATAAGAG
++
+EEEEEEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:16709:1904___1:N:0:TCCTGAGC+TACTCCTT;FP:5671;RQ:34.73
+GTTCCATGCGTCAATGTTTCTTCTGGATACAGCAGGCCTGTTCCCGGGAGTCAATGC
++
+EEEEEAEEEEEEEEEEAEEEEEEEEEEEAEE6<EEEEEAAA<EAEEEEEEEEEEE<E
+@NB500968:8:H5CFGAFXX:1:11101:12189:1919___1:N:0:TCCTGAGC+TACTCCTT;FP:53204;RQ:34.62
+ACACCCTTTATAGTGATGGATTGGATCTGTTTGAAGGCAAAGTCCTTCCCAGACTTATCA
++
+EEEEEEE/EEE/EEAEEEEEEEEEEE/EE<EAAAE/E<</EA///A</E/A/AAA<A/</
+@NB500968:8:H5CFGAFXX:1:11101:26750:1960___1:N:0:TCCTGAGC+TACTCCTT;FP:578;RQ:35.03
+GGGAGCGTCAGATGTGTATAAGAGACAGCTGACCC
++
+EEEAEEEAEEAEAEAEE/EEEEAEAAEEEEE/EEE
+@NB500968:8:H5CFGAFXX:1:11101:21600:1964___1:N:0:TCCTGAGC+TACTCCTT;FP:38123;RQ:18.05
+GAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAA
++
+EEEEEEAEE/AAAEEEEEEEEEEEEEE<EEEAEEEEAAAEAAEEEE<EA<A<EA<<E<E
+@NB500968:8:H5CFGAFXX:1:11101:11625:2006___1:N:0:TCCTGAGC+TACTCCTT;FP:4030;RQ:35.03
+GACAGCGTCAGATGTGTATAAGAGACAGCAACCACC
++
+AEA66E/<AEAEE/E/EEAEEEEAEEEEEAEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:10474:2017___1:N:0:TCCTGAGC+TACTCCTT;FP:16506;RQ:35.03
+GGAGCGTCAGATGTGTATAAGAGACAGTCGTCC
++
+EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:17381:2018___1:N:0:TCCTGAGC+TACTCCTT;FP:64754;RQ:35.18
+AACGTCAGATGTGTATAAGN
++
+EEE/EEEAEEEEEAE</EA#
+@NB500968:8:H5CFGAFXX:1:11101:9520:2027___1:N:0:TCCTGAGC+TACTCCTT;FP:57159;RQ:34.81
+AGCGTCAGATGTGTATAAGAGACAGCTACATCCCTATAAGGATGTAC
++
+EEEEEEEEEEEEEEEEAEEEEEEEEEEAEEEEEEE/EEEEE<AEEEA
+@NB500968:8:H5CFGAFXX:1:11101:23302:2031___1:N:0:TCCTGAGC+TACTCCTT;FP:9055;RQ:34.81
+AGCGTCAGATGTGTATAAGAGACAGATCGTCCCTATAGCTCCAATC
++
+EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEAEEEEEEAEE
+@NB500968:8:H5CFGAFXX:1:11101:19677:2089___1:N:0:TCCTGAGC+TACTCCTT;FP:14776;RQ:34.63
+GGCGCCAGTCCTCCGATTGACTGAGTCGCCCGGGTACCCGTGTATCCAATAAACCCTCTT
++
+EEEEEEEAEEEEEEEAE/EEEEEEEEAEEE//E/EEE/A<AAAAAA<AEAE/EE/AAA<6
+@NB500968:8:H5CFGAFXX:1:11101:26158:2095___1:N:0:TCCTGAGC+TACTCCTT;FP:23868;RQ:34.83
+GGGAGCGTCAGATGTGTATAAGAGACAGCCACTCCCTATAGGATTTCC
++
+EEEEEEEEEAEEEEEEEEEEEEEEEEEEA<EEEEAEEA<6EEEEE<EA
+@NB500968:8:H5CFGAFXX:1:11101:7883:2101___1:N:0:TCCGGAGC+TACTCCTT;FP:59501;RQ:34.83
+GGAGCGTCAGATGTGTATAAGAGACAGCATCACCCTATAGGCTGTCT
++
+EEEEEEEEEEEEEEAEAEEEEEEEEEEEEEEEEEEEEE<EEEEEEAE
+@NB500968:8:H5CFGAFXX:1:11101:24496:2131___1:N:0:TCCTGAGC+TACTCCTT;FP:247;RQ:35.19
+GGGAGCGTCAGATGTGTATAAG
++
+EEEEEEEEEEE/EEEEEEE/AE
+@NB500968:8:H5CFGAFXX:1:11101:5814:2162___1:N:0:TCCTGAGC+TACTCCTT;FP:5751;RQ:34.63
+GCCCTTGGCTTCACCACACTTTCTACTGGAAATGTGCCTTTTCCTCCATTGCTGCTAC
++
+EEEEEEEEEEEEEEEAAEEEAEEEEEA/EEEEEE<EEEAEAAEEAEEE<AAEAEEAAE
+@NB500968:8:H5CFGAFXX:1:11101:5796:2184___1:N:0:TCCTGAGC+TATTCCTT;FP:4250;RQ:34.83
+GGAGCGTCAGATGTGTATAAGAGACAGGCTGTCCCTATAGACTGACT
++
+EEEEEEEEEEEEEEEAEAEEEEEEEEEEEEEEEEAEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:23937:2223___1:N:0:TCCTGAGC+TACTCCTT;FP:26022;RQ:34.63
+GCTTCTTCTGAAGAGCTCCACCTGTGGTCTCTCCACCTCTCGAGGCTCTTCACTAGCCT
++
+EEEEAEA/AEEEEEEEA/</</</A//EAEEE<AE66A666/A/E6E/AAAAA////E/
+@NB500968:8:H5CFGAFXX:1:11101:25267:2246___1:N:0:TCCTGAGC+TACTCCTT;FP:4173;RQ:34.83
+GAGCGTCAGATGTGTATAAGAGACAGGATGCCCCTATATATAGAGGCTG
++
+E6EEEEAAEEEEEEEAEEEEEEEEEEEEEEE6EEEEEEEEEAEAEEAEA
+@NB500968:8:H5CFGAFXX:1:11101:19822:2253___1:N:0:TCCTGAGC+TACTCCTT;FP:41486;RQ:34.63
+GACGTGCAAATCGGTCGTCCGACCTGGGTATAGGGGCGAAAGACTAATCGAACCATCTAG
++
+EEEEEEEEEEEEEAEEEEEEEEEEEEAEEEEEAEAEE/EEEEEEEEE<<EEEEAEEEA//
+@NB500968:8:H5CFGAFXX:1:11101:6670:2262___1:N:0:TCCTGAGC+TACTCCTT;FP:14539;RQ:3.28
+GGAGGCCAAATTGTGCTGATTTTCCTGTAGCTGCGATTAATAGGCCTATAAGTGGAATTA
++
+EEEEEEEEEEAEAEEAEEEEEEEE/EAEEEAEEAEEAE/EEAE/</E/EA66A<//<EE/
+@NB500968:8:H5CFGAFXX:1:11101:14858:2304___1:N:0:TCCTGAGC+TACTCCTT;FP:4202;RQ:34.63
+GTCTCCGGCTTGTGTAGACTCTGCCTGGAAGTGAAGTTGTGGACTCCCGACCTCCAGTC
++
+EEEEEEEEEEEEEEEEEEEEEA/E/AEEEEEAEEEEAE/<AAEEE<EAEEE<EEAEEEE
+@NB500968:8:H5CFGAFXX:1:11101:10463:2345___1:N:0:GCCTGAGC+TACTCCTT;FP:24337;RQ:34.81
+TCCAGCGTCAGATGTGTATAAGAGACAGAGTTCCCCTATAGAGAGACG
++
+EEEEEEEEEEE6EEEEA/A//E/EEEEEEEEEEEEEEAA/AAEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:24405:2351___1:N:0:TCCTGAGC+TACTCCTT;FP:5580;RQ:35.18
+TCTGTCTCTTATACCNNNNN
++
+EEEEEEAEEEEEEEE#####
+@NB500968:8:H5CFGAFXX:1:11101:6508:2370___1:N:0:TCCTGAGC+TACTCCTT;FP:64422;RQ:34.83
+GGAGCGTCAGATGTGTATAAGAGACAGCCCAGCCCTATACCGTCTAGCTG
++
+EEEEEEEEEEEEEAEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:21730:2488___1:N:0:TCGTGAGC+TACTCCTT;FP:57284;RQ:35.18
+AGCGTCAGATGTGTATCNNN
++
+EEEEEEEEEEEEEAEAE###
+@NB500968:8:H5CFGAFXX:1:11101:5499:2506___1:N:0:TCCTGAGC+TACTCCTT;FP:0;RQ:1.56
+NNNNNNNNNNNNNNNNNNNN
++
+####################
+@NB500968:8:H5CFGAFXX:1:11101:20021:2516___1:N:0:TCCTGGGC+TACTCCTT;FP:28351;RQ:34.83
+GGAGCGTCAGATGAGTATAAGAGACAGTCATCCCCTATAGTCGTTCA
++
+EAEEAAEEAAE///E/EAAE/EAEE//EEE/EEEEEE/EE/EA6EEE
+@NB500968:8:H5CFGAFXX:1:11101:4827:2579___1:N:0:TCCTGAGC+TACTCCTT;FP:8626;RQ:34.83
+GACAGCGTCAGATGTGTATAAGAGACAGGGAATCCCTATAGACTTTGC
++
+EEEEEEEEE/E<AAAEEEEEEEE/E/EEEEEEEEEEEAEEAEAAEEE/
+@NB500968:8:H5CFGAFXX:1:11101:25015:2619___1:N:0:TCCTGAGC+TACTCCTT;FP:0;RQ:1.56
+NNNNNNNNNNNNNNNNNNNN
++
+####################
+@NB500968:8:H5CFGAFXX:1:11101:11758:2662___1:N:0:TCCTGAGC+TACTCCTT;FP:32308;RQ:34.83
+GACAGCGTCAGATGTGTATAAGAGACAGAGATGCCCTATAGACCGAAC
++
+EEEEEE/EAE/EEE/EEEAEE6E6EEEEEAAEA/E/AEEEA/E6EA/E
+@NB500968:8:H5CFGAFXX:1:11101:25905:2738___1:N:0:TCCTGAGC+TACTCCTT;FP:0;RQ:1.56
+NNNNNNNNNNNNNNNNNNNN
++
+####################
+@NB500968:8:H5CFGAFXX:1:11101:23131:2747___1:N:0:TCCTGAGC+TACTCCTT;FP:61606;RQ:34.61
+CAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCGAGGTTATCTA
++
+EEEEEEAEAEEEE/EEEAEEEEA/EEEEAEA/EA<EAAAEEAE<AE/A<<EEEA<<EEA<
+@NB500968:8:H5CFGAFXX:1:11101:20373:2751___1:N:0:TCCTGATC+TACTCCTT;FP:283;RQ:34.93
+GGAGCGTCAGATGTGTATAAGAGACAGCCCTATACAGTGATC
++
+EEEEEEEEE<EEEEAEAEEEEEEEEEEEEEE/EEEEEE<EAA
+@NB500968:8:H5CFGAFXX:1:11101:21204:2795___1:N:0:TCCTGAGC+TACTCCTT;FP:16279;RQ:34.81
+AGCGTCAGATGTGTATAAGAGACAGCTCCACCCTATAGGCCCTAACTG
++
+EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:10694:2817___1:N:0:TCCTGAGC+TACTCCTT;FP:10036;RQ:34.83
+GGAGCGTCAGATGTGTATAAGAGACAGCCCTTCCCTATACGCTTGGCC
++
+EEEEEEEEEEEEE<EEEEAEEEEEEEEEE/A<E<EEEA/EEEAEEEE/
+@NB500968:8:H5CFGAFXX:1:11101:8407:2853___1:N:0:TCCTGAGC+TACTCCTT;FP:21171;RQ:34.62
+AGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTT
++
+/EEEEEEAEEEEEAEEEEEEEEEAEAEEAAEEEE/EEEAAAEA//EEEAEAAA/EAEAE6
+@NB500968:8:H5CFGAFXX:1:11101:17699:2880___1:N:0:TCCTGAGC+TACTCCTT;FP:52710;RQ:34.62
+CTTTGGAATCTTCACAAGCAGAACCAGTCAAAACCCCGGCAAGCACAAAGAGACTCTCCA
++
+EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE<EEEEEEEEEEE6AEAEAEEEEA
+@NB500968:8:H5CFGAFXX:1:11101:2261:2941___1:N:0:TCCTGAGC+TACTCCTT;FP:2596;RQ:34.63
+GGCAGCATCAGATGTGTATAAGAGACAGAAGGAGCAGAGAGCGGCCAGCAACCGATGAGA
++
+EEEEEEEEEEEEEEAEEEAEEEEE///////EA//E/E/A/////E<EE//</E<<////
+@NB500968:8:H5CFGAFXX:1:11101:11672:2944___1:N:0:TCCTGAGC+AACTCCTT;FP:32501;RQ:34.63
+GGGTGAAATACTGTGAGGAACCCAGAAGTGAGTGGAAAGATAACTGGCTTTTTTTTTTTT
++
+AEEEEEEEEEEEAAAEEEEEAA<E/EEEEEEEAAEAEEEE<EEE<</EE6AA/6/<66//
+@NB500968:8:H5CFGAFXX:1:11101:15968:2945___1:N:0:TCCTGAGC+TACTCCTT;FP:8706;RQ:34.63
+GCAAATCGGTCGTCCGACCTGGGTATAGGGGCGAAAGGCTAATCGAACCATCTAGTAG
++
+EEEEEEEAEEEEEEEEEEEEEEEEAEEEEEEAEEEEE<EEEEAAEAEEAE<AEAEEEE
+@NB500968:8:H5CFGAFXX:1:11101:25690:2959___1:N:0:TCCTGCGC+TACTCCTT;FP:27004;RQ:34.81
+AGCGTCAGATGTGTATAAGAGACAGGCCTCCCCTATAGCTTCGGA
++
+AE6EEEEEEEEEAAEEEEEEEEEEEEEEAEE/E/EAEEEEAEEEE
+@NB500968:8:H5CFGAFXX:1:11101:8090:2972___1:N:0:TCCTGAGC+TACTCCTT;FP:12902;RQ:34.81
+CAACGTCAGATGTGTATAAGAGACAGGGACCCCCTATAATTTATGCC
++
+EEEEEEEEEEEEEEEEEAAEEEEEEEEEAEE<EEEEEAE<EEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:22125:3141___1:N:0:TCCTGAGC+TACTCCTT;FP:65197;RQ:35.07
+AGCGTCAGATGTGTATAAGAGACAGCTC
++
+EEEEEEEEEEEEEEEEEEEEEEEAEEEE
+@NB500968:8:H5CFGAFXX:1:11101:6707:3162___1:N:0:TCCTGAGC+TACTCCTT;FP:0;RQ:1.56
+NNNNNNNNNNNNNNNNNNNN
++
+####################
+@NB500968:8:H5CFGAFXX:1:11101:24232:3202___1:N:0:TCCTGAGC+TACTCCTT;FP:19483;RQ:34.63
+GTTGGTTTTCGGAACTGAGGCCATGATTAAGATGGACGGCCGGGGGCATTCGTATTGCG
++
+EEEEEEEEEEAEEEEEEE/E<EEEEEA</EEE/A/EE<E666<EEAEEEE/6/A66/66
+@NB500968:8:H5CFGAFXX:1:11101:16936:3211___1:N:0:TCCTGAGC+TACTCCTT;FP:7401;RQ:34.83
+GAGCGTCAGATGTGTATAAGAGACAGTGCGTCCCTATAAAAGTGAACTG
++
+EEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEEEEEEA/EEEAEAEEE
+@NB500968:8:H5CFGAFXX:1:11101:6680:3212___1:N:0:TCCTGAGC+TACTCCTT;FP:62506;RQ:34.81
+TCCAGCGTCAGATGTGTATAAGAGACAGCTCCTCCCTATAAG
++
+EEEEEEEEEEEEEEEEEEEAEEEEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:6132:3257___1:N:0:TCCTGAGC+TACTCCTT;FP:7557;RQ:34.62
+CGTCAGATGTGTATAAGAGACAGCATTCCCCTATACACGCCTCCTGTCTCTTATAC
++
+EEEEAEEEEEEEEEEEEEEAEAEEAEEEEAEEEEEEEEEEEEEEEEAE/EEEEEEA
+@NB500968:8:H5CFGAFXX:1:11101:3840:3285___1:N:0:TCCTGAGC+TACTCCTT;FP:16156;RQ:34.93
+GGGAGCGTCAGATGTGTATAAGAGACAGGTATACCCTATAG
++
+EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:2858:3312___1:N:0:TCCTGAGC+TACTCCTT;FP:14452;RQ:35.12
+AACGTCAGATGTGTATAAGAG
++
+EE/AE/E/EAAEEEEEEEAEE
+@NB500968:8:H5CFGAFXX:1:11101:3641:3398___1:N:0:TCCTGAGC+TACTCCTT;FP:36051;RQ:35.14
+GCAGCGTCAGATGTGTATAAGAG
++
+EEEEEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:20540:3401___1:N:0:TCCTGAGC+TACTCCTT;FP:48603;RQ:34.81
+AGCGTCAGATGTGTATAAGAGACAGATCAACCCTATATGCCCTAACTG
++
+E/E///E/E</A/EEA<E/E/EEE/E</E///A/E6<E/A/<E/EEE/
+@NB500968:8:H5CFGAFXX:1:11101:19438:3463___1:N:0:TCCTGAGC+TACTCCTT;FP:0;RQ:1.56
+NNNNNNNNNNNNNNNNNNNN
++
+####################
+@NB500968:8:H5CFGAFXX:1:11101:4772:3477___1:N:0:TCCTGAGC+AACTCCTT;FP:61788;RQ:3.33
+TAACTGTCTCTTATACACCN
++
+EEEEEEEAEEEAEEE<EEE#
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/read1_un.fq	Mon May 09 04:08:01 2016 -0400
@@ -0,0 +1,72 @@
+@NB500968:8:H5CFGAFXX:1:11101:24482:1380 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.00
+GTGTATAAGAGACAGGCCTAATATATAGGGGGCGCCAGTCCTCCGATTGACTGAGTCGCCCGGGTACCCGTGTAT
++
+AAAAAEE/E/E/<EEEE/EAEE<EEEEEEEEEEEEEAEEA/E/EAEEEEEEEEEEEEEEE<E/EE<<6EEAEEEE
+@NB500968:8:H5CFGAFXX:1:11101:2671:1393 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.15
+GGTCCCCTATAAGGGGATTCTGTCTCTTATACCNN
++
+///AA6E/6AA///EAE//E6E6E6E/6AAE/E##
+@NB500968:8:H5CFGAFXX:1:11101:7850:1487 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.68
+ATTCCAATATAGGGGACCCACATCTTCCTCTGCTTCTAGATGAAGGATGTATTCTTCTTCCTCTTCTTCCTCTG
++
+AAAAAEEE/EEE/EEEEEEAAEEE/EEEEEAEEEEEEEEEEEEEEAEAE6EEA<EEEAEE<6E/<EE<<E6E/<
+@NB500968:8:H5CFGAFXX:1:11101:13010:1719 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.00
+AAGACAGTGTGATTATATAGGGGACAGCGTCAGATGTGTATAAGAGACAGCTAAGCCCTATACGTTTTTTC
++
+/A/AAEAAEEEE/E/EAEAAEE</EEEEEEAEEEEAAEAAAEAEEEAEEEEE/EE/EEE6A/EEEAEEEEA
+@NB500968:8:H5CFGAFXX:1:11101:15935:1725 1:N:0:GCCTGAGC+TACTCCTT;RQ:0.00
+GTGTATAAGAAACAGGCTTTATATAGGGGCACCGTCAGATGTGTATAAGAGACAGTTGTCCCCTATACCACGC
++
+/AAAAEEEEAEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE/E<<EEEEE/EE<EEEE/AEEEEEEEAEEE
+@NB500968:8:H5CFGAFXX:1:11101:12108:1890 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.02
+CAGTTAGGTGTTATAGGGATCCCAAGACAAGGTTTCTCTGTGTCTATCCCTGGCTGTCCTTGAACTTCGGCAT
++
+6AAAA66EAE6EEEEEAE/EAAEEE/EEEEAEEEEEE/EEEEE/EAEEE/E/EAEEE<<EE/EAEEE6/EEE<
+@NB500968:8:H5CFGAFXX:1:11101:6961:1984 1:N:0:TCCTGAGC+TACTCCTT;RQ:17.82
+GGATGTCGCTATAGGGCGCCGCCGCCTCCTCCTCCTCTCTCGCGCTCTCTGTCCCGCCTGGTCCTGTCCCACCC
++
+AAAAAAEEEEEEEEEEEEEAEAAEEE</EEEE/EEEEEEAEEEAAEEEEEEEEEEEEEEAEEEAEEEEAE/EEE
+@NB500968:8:H5CFGAFXX:1:11101:20646:1998 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.00
+CCGCAAGTGCGTTCGAAGTGTCGATGATCAATGTGTCCTGCAATTCACATTAATTCTCGCAGCTAGCTGCGTT
++
+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEAEEEEEEAEEEEEEEEEEEEEEEEAAEE
+@NB500968:8:H5CFGAFXX:1:11101:19922:2125 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.08
+GTATAAGAGACAGCGATACTTTATAGGGGGGCCGTGTGCAGACCCGCGTGTGGCGCAGGCAAGGACCCTGAAAAT
++
+AAAAAEE/EAEEEEEEEEEEEEEEEE/EEEEEEEEAEEEEEEEEEEEEAEEA<<EEEEEEEEEEEEEEEEEEEA<
+@NB500968:8:H5CFGAFXX:1:11101:14935:2297 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.05
+GATGTGCATAAAAGACAGATAGTACGTATAGGGGGGAGCGTCAGATGTTTATAAGAGACAGAATCGCCCTATAG
++
+AAAAAAAEEAEEEEEEEEEEEEEEEEEEEEEEEAEEEEEAE/A/EEAE/E/6/E///E/E/</E///A/AA/</
+@NB500968:8:H5CFGAFXX:1:11101:13250:2472 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.00
+GTCTCCGCCCTTGCTGACTTTCCATCATTTTTAAGATCAGGTGGAACTGGAGCCTCTGCCTGTGGCTGATCTGAG
++
+AAAAAEAA/EAA<EEEEE/EEAAEEAE<EEA/E//EE/E/EEAAEEEEA6A/6EAA6/6<6/<//A/<AAE6AA/
+@NB500968:8:H5CFGAFXX:1:11101:8485:2792 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.00
+CGTCAGATGTGGTCCCGCTCACCATGCCGCTGCTGTAGCCGCTCACCAACCTCCTGTGCCCCCCGCAACTCAT
++
+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEE6EEEEEEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:23310:3069 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.02
+CAGGCCAATCGTATAGGGGTTGATACTCAAGTCTACTTGTTCCCTTTTATTTAATCCTGAACCCCATCCCATGGA
++
+AAA/AEEEEEEEAE//EEE////EAA//EE/EEEEEE//EEE/E/E/EEEEEEE<A<E/AEE//AE/<AAEEEE/
+@NB500968:8:H5CFGAFXX:1:11101:9254:3110 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.00
+TGTTATAAGGCGTCAGATGTGTATAAGAGACAGGGATCCCCTATAAAGAGCCTAAC
++
+AAAAAEEEEEEEEEEEEEEEEEEEAEEEE<EAEEE/EEEEEEEEE/EEEEEEEAA/
+@NB500968:8:H5CFGAFXX:1:11101:4099:3187 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.00
+GTATAAGAGACAGCCAGCCTCTATAGGGGTGCTTGCAGGGGAGGGAAAAGGGGGAAAGCGGGCAACCAGTTTCC
++
+AAAAAAEAE6E6EEEEEAEEA<EEEEEEEEEEEEEEEEEEEEEEEEEEAEE/EEAEEEEEEEEEEEEEAE/E//
+@NB500968:8:H5CFGAFXX:1:11101:15733:3232 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.15
+TCCCCTATATTTGGGGACTGTCTCTTATACACCNN
++
+AAAAAEEE/EEEEEEEEEEEEEEEEEEEEEEEE##
+@NB500968:8:H5CFGAFXX:1:11101:3009:3242 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.00
+GGTTAAGGCTAGCCTATTCTACATAGAGTGTTCTAGGCCTTCCAGGGCCACATAGACTCTTGTTTCAAAAAAAT
++
+A//AAEEEEEEEEEEEAEE/EEEEAAEE/AAEEEAEEEEAEEEEAEEEEEEEEEEEEEEA6EEEE<EEE/A/EA
+@NB500968:8:H5CFGAFXX:1:11101:18503:3442 1:N:0:TCCTGAGC+TACTCCGT;RQ:0.07
+TAATCCCTATAAGGGATTACTGACTCTTATACATGTATCTAATACACATCTCCGAGCCCACGAGACTCCTGAGCA
++
+AAAAAE/AAEEEEEEAAE/E//////EEAA///A/E/<///AEEEE/E/EE<<///E<EEE/E/AE6/EEEE//6
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/read2_extracted.fastq	Mon May 09 04:08:01 2016 -0400
@@ -0,0 +1,328 @@
+@NB500968:8:H5CFGAFXX:1:11101:15939:1071___1:N:0:TCCTGAGC+TACTCCTT;FP:23586;RQ:34.63
+NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
++
+###################################
+@NB500968:8:H5CFGAFXX:1:11101:1121:1139___1:N:0:TCGTGAGC+TACTCCTT;FP:27828;RQ:34.83
+GATGTAGACTATAGGGAATACCNNNNNNNNNNNNN
++
+A6AAAEEEEEEEEEEEAEE/E<#############
+@NB500968:8:H5CFGAFXX:1:11101:7529:1175___1:N:0:TCCTGAGC+TACTCCTT;FP:17358;RQ:34.81
+GATGTGTAAAAGAGACAGAGGATAATTATAGGGATCCTGTCTCTAAAACACATCTGACGCTCCCTATACATAATA
++
+/AAAAE<A/E//EEE/EE//EEEEAEEEEEEEEEE/A/<EEE<<///<AE</AE//AAA//E/////EEE<6/A/
+@NB500968:8:H5CFGAFXX:1:11101:5052:1198___1:N:0:TCCTGAGC+TACTCCTT;FP:38799;RQ:34.63
+GGATTGAGGCTAGAAACAGCTGCTTTTCTCACTTTCCCAGCAGCAAGACATCAATCTGTTGAGACCTCTCATTTT
++
+AA6AAEEAEEEEEEEEAAEEEEE///AEEA/<E/E<A//EEEEAE/EE//AE///E</EAEEA<EEEAEE/E/E/
+@NB500968:8:H5CFGAFXX:1:11101:15805:1205___1:N:0:TCCTGAGC+TACTCCTT;FP:44542;RQ:34.63
+GCGTCAGATGTGGGTAGTCAGAAAAGCAGATGGATGGGGGCCTTGCCCAACCAGATCCAGGCCGAGTTCACCT
++
+AAAAAEEAEEEEEEEEEEEEEEA//EEEEEEEEEEEEEEEEEEAEE/E//EEEEAEAE/EEEEE<EEEEEEEA
+@NB500968:8:H5CFGAFXX:1:11101:11834:1217___1:N:0:TCCTGAGC+TACTCCTT;FP:56795;RQ:34.81
+GGGTCGCTATATAGGGGAATCCNNNNNNNNNNNNN
++
+AAAAAEEEEEEEEEEEEEAEEE#############
+@NB500968:8:H5CFGAFXX:1:11101:20105:1230___1:N:0:TCCTGAGC+TACTCCTT;FP:2265;RQ:34.63
+GTACACGTATTACTTCTGTTTGGTGAATTAAATAAAACAATTCCTAAGCAACTCAAAAGTAACCTAAAAATTGCA
++
+AA/A//EEE/EAEEEEEEE/EEEAEE6EEE<EEA<6E//E/EEAEE//EE/EEE<EAEE6</EEEEE/A/EEE/A
+@NB500968:8:H5CFGAFXX:1:11101:14524:1264___1:N:0:TCCTGAGC+TACTCCTT;FP:7937;RQ:2.65
+GTATAAGAGACAGTTGGGACTTATAGGGTAAGTCN
++
+AAAAAEEEAEEEEAEEE/EEEEEAE<EE/EEEEE#
+@NB500968:8:H5CFGAFXX:1:11101:14981:1265___1:N:0:TCCTGAGC+TACTCCTT;FP:26333;RQ:34.63
+GGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCG
++
+AA6AAEE/EE//EEE6EEEEEEEE/AAEEAEEEE/EEEEEAEEEE/EEEEEEEEEEEE/AEE<EEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:5538:1286___1:N:0:TCCTGAGC+TACTCCTT;FP:5079;RQ:34.62
+ATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCACTATTTTGCCACATAGACGAGTTGATT
++
+AAA/AEE/EEEEEEEAEEEEEEEE/EEEEE/EEEA</EEEEEEEEAAAEAEEEEE<EAE6EEEEEEEEEEEEAE
+@NB500968:8:H5CFGAFXX:1:11101:11473:1333___1:N:0:TCCTGAGC+TACTCCTT;FP:48246;RQ:35.03
+NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
++
+###################################
+@NB500968:8:H5CFGAFXX:1:11101:2141:1369___1:N:0:TCCTGAGC+TACTCCTT;FP:0;RQ:1.56
+GATGTGTATAAGAAACAGACCACATATATAGGGGGGAGCGTCAGATGTGTATAAGAGACAGCCCCCCCCAATAGA
++
+AAAAAEEEEEEEAEEEEE/E/EAEEEEEAEEEA/EEEEEEEAA//AEEEE/</AA/////////AAEE////AA/
+@NB500968:8:H5CFGAFXX:1:11101:19474:1422___1:N:0:TCCTGAGC+TACTCCTT;FP:5233;RQ:35.03
+NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
++
+###################################
+@NB500968:8:H5CFGAFXX:1:11101:12411:1441___1:N:0:TCCTGAGC+TACTCCTT;FP:46039;RQ:34.83
+GACAAAATATAGGGGGACTGTCTGTTATAGACGTCGGACGCGCGCCCCTATAAGGAATAGGGGTCTCTGAGAGA
++
+A/////E////////A///E/E///EEE///A//A/A//<///////E/////AEA//////////////////
+@NB500968:8:H5CFGAFXX:1:11101:5889:1458___1:N:0:TCCTGAGC+TACTCCTT;FP:52002;RQ:34.83
+CAGCGAAGGGGTATAGGGATACGNNNNNNNNNNNN
++
+A/AAA//EEEEA/A/EEE////E############
+@NB500968:8:H5CFGAFXX:1:11101:8662:1469___1:N:0:TCCTGAGC+TACTCCTT;FP:62135;RQ:35.03
+NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
++
+###################################
+@NB500968:8:H5CFGAFXX:1:11101:9851:1471___1:N:0:TCCTGAGC+TACTCCTT;FP:43356;RQ:3.17
+GTATAAGAGACAGGGGCCCGTTAAAGGGCCCNNNN
++
+/A6<//EA//E/E/A/EEE/E/E/E/6EEE/####
+@NB500968:8:H5CFGAFXX:1:11101:5425:1488___1:N:0:TCCTGAGC+TACTCCTT;FP:13390;RQ:34.83
+GTATAAGAGACAGTGTGTCCATATAGGGGAGTCNN
++
+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEE##
+@NB500968:8:H5CFGAFXX:1:11101:21998:1542___1:N:0:TCCTGAGC+TACTCCTT;FP:15180;RQ:35.09
+NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
++
+###################################
+@NB500968:8:H5CFGAFXX:1:11101:2814:1550___1:N:0:TCCTGAGC+TACTCCTT;FP:0;RQ:1.56
+CCGTCAGATGAGTATAAGAGACAGGTAAGACATAAAGAGCGTAGTTGTCTCTAATACACATCTGACGCTGCCGA
++
+/AAA6AEEE//E/EAAEE6/EEEEEEEE//EE/E/EE/AE/<A//</AE/E////AE<A/<A/6/E<EEAAEEE
+@NB500968:8:H5CFGAFXX:1:11101:8024:1581___1:N:0:TCCTGAGC+TACTCCTT;FP:38311;RQ:34.63
+GAACTATGACTGTGAGCAGGCATTGTGGTTCTGCCCAAGCTACATCCAGATCACCCAGGCCTCTGGGTCTTTTGG
++
+AAAA6EAEE/E/EEEEEEEAEEEEEEEAEEEEAEEEEEAE6EEE6EEEAA/EEEEEE/A/E/AEAEE<EEEEA/<
+@NB500968:8:H5CFGAFXX:1:11101:8843:1628___1:N:0:TCCTGAGC+TACTCCTT;FP:44455;RQ:34.63
+GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAA
++
+AAAAAEEEEEEEEEEEEEEEEEAEEEEEEEEEEEEEEEEEAEEEEEEEEEE<EEEEEEEEEEEE<AA/E6EEAA6
+@NB500968:8:H5CFGAFXX:1:11101:23474:1673___1:N:0:TCCTGAGC+TACTCCTT;FP:2968;RQ:34.81
+GATGTGTATAAGAGACAGATATAGACTATAGGGCGCCT
++
+A/AA////A//EA/6<EE6/EEE6////A//EE/E6//
+@NB500968:8:H5CFGAFXX:1:11101:1482:1752___1:N:0:TCCTGAGC+TACTCCTT;FP:20529;RQ:35.03
+NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
++
+###################################
+@NB500968:8:H5CFGAFXX:1:11101:21720:1786___1:N:0:TCCTGAGC+TACTCCTT;FP:16257;RQ:35.14
+GTCTCTTATACACATCTGACGCTCCCCCCTATAGTTCAAAT
++
+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:9012:1809___1:N:0:TCCTGAGC+TACTCCTT;FP:4167;RQ:34.83
+GTGTATAAGAGACAGGCCTATGATATAGGGGGTAC
++
+AAAAAEEEEEEEEEEEEEAEE/EEEAE/AAEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:12567:1818___1:N:0:TCCTGAGC+TACTCCTT;FP:61471;RQ:34.63
+GGTCATGGGAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGT
++
+AA/AAEEAEEE//AEAEEEEEEEEEEAEEEEEEEE/EAEE<EE<EA/<E<EEEEE6EEEE/EEEEEE/EEAEE
+@NB500968:8:H5CFGAFXX:1:11101:20110:1819___1:N:0:TCCTGAGC+TAATCCTT;FP:64559;RQ:34.63
+GAAGAGGGTATCAGATCCCTTGGATCTGGAGTCGCAGATGGCTGCGAGCCACCATGTGGGTTTTGAGAATTGAAG
++
+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEEEAEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:24842:1887___1:N:0:TCCTGAGC+TACTCCTT;FP:13772;RQ:35.14
+CTCATAAACACATCAGGCGGACCCCGCAAAATAAAGGATCTATCTCTGATAAAGAACTGACGCTGCCGACGAAA
++
+A///AE/6EE///////E//6E/AE/<////EE/A/A/A////EAA///////////E/EE/E/////A//E/A
+@NB500968:8:H5CFGAFXX:1:11101:16709:1904___1:N:0:TCCTGAGC+TACTCCTT;FP:5671;RQ:34.73
+CGGATCGCTTCTTCTGATTGAGTTGCCACTCTTTGAAATGCGGCACGGCCTCGTCCACGTTGACGATGCCCAG
++
+AAAAAEEEEEEEAEEEEEEEEEEEEEEEEEEE/EEEEAEEEEEEEEEEEEEEEEE//EEEEEEEAEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:12189:1919___1:N:0:TCCTGAGC+TACTCCTT;FP:53204;RQ:34.62
+GTCTATAGGTATCAGTTTCACTGTAATACCTTTCCTTCCATGGCTAAGTCTGGGAAGGACTTTGCCTTCAAACAG
++
+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE<AEEEEEEEEE<E<EAEAEEEEEEEEEEA
+@NB500968:8:H5CFGAFXX:1:11101:26750:1960___1:N:0:TCCTGAGC+TACTCCTT;FP:578;RQ:35.03
+NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
++
+###################################
+@NB500968:8:H5CFGAFXX:1:11101:21600:1964___1:N:0:TCCTGAGC+TACTCCTT;FP:38123;RQ:18.05
+GCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACG
++
+AAAAAEEEEEEAEEEEE/E/EEE6/EEEEEEEE/EEEEEEAEEEEEEAEEEAEEEEEEEEEAEE<EAEAEEE
+@NB500968:8:H5CFGAFXX:1:11101:11625:2006___1:N:0:TCCTGAGC+TACTCCTT;FP:4030;RQ:35.03
+NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
++
+###################################
+@NB500968:8:H5CFGAFXX:1:11101:10474:2017___1:N:0:TCCTGAGC+TACTCCTT;FP:16506;RQ:35.03
+NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
++
+###################################
+@NB500968:8:H5CFGAFXX:1:11101:17381:2018___1:N:0:TCCTGAGC+TACTCCTT;FP:64754;RQ:35.18
+CTTATACACATCTGACGTTCCCTATACTAATAAAN
++
+A/AAAAE6E////E/AEAE/AEEAEA/EA/EEEE#
+@NB500968:8:H5CFGAFXX:1:11101:9520:2027___1:N:0:TCCTGAGC+TACTCCTT;FP:57159;RQ:34.81
+GTACATCCTTATAGGGATGTAGCNNNNNNNNNNNN
++
+AAAAAEEEEAEEEEEEEEAEEEE############
+@NB500968:8:H5CFGAFXX:1:11101:23302:2031___1:N:0:TCCTGAGC+TACTCCTT;FP:9055;RQ:34.81
+NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
++
+###################################
+@NB500968:8:H5CFGAFXX:1:11101:19677:2089___1:N:0:TCCTGAGC+TACTCCTT;FP:14776;RQ:34.63
+CTCCTCTAAGGGGGGTGCACCAAAGAGTCCAAAACGATCGGGATTTTTGGACTCAGGTCGGGCCACAAAAACGG
++
+AAAAAA/EEEEEEEEEEE/EEEAEEEEEEE/AAAEA/<EEEEE/E</<<E/EEEE/E6EEAEAE/EEE///EEA
+@NB500968:8:H5CFGAFXX:1:11101:26158:2095___1:N:0:TCCTGAGC+TACTCCTT;FP:23868;RQ:34.83
+GATGTGTATAAGAGACAGGGAAATCCTATAGGGAGTGG
++
+AAAAAEEEEEEEEEEEAEEEAEAEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:7883:2101___1:N:0:TCCGGAGC+TACTCCTT;FP:59501;RQ:34.83
+GTATAAGAGACAGAGACAGCCTATAGGGTGATGCN
++
+AAA/AEEE/EE/EAAEE//EEA/E/EAAEE/E6<#
+@NB500968:8:H5CFGAFXX:1:11101:24496:2131___1:N:0:TCCTGAGC+TACTCCTT;FP:247;RQ:35.19
+CTTATACACATCTGACGCTCCCCCCTATAAGAATTTT
++
+AAAAAAEAAE//EEEEEEEE/EEEEEEEAAAEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:5814:2162___1:N:0:TCCTGAGC+TACTCCTT;FP:5751;RQ:34.63
+CTCCTACAGGACAGAAGGACTCTGCTGCCTGTTGCCAAGGAAGGCAGAGCTAGCTGAACTGCGTGTAAAAACGGG
++
+AAA/AEA/EEEEEEEEEEE/EAEEAE<EEA/EAEAA<EEEE/EEEEAEAEEEAEEAEAEEAAAEAEEE/E/<A<<
+@NB500968:8:H5CFGAFXX:1:11101:5796:2184___1:N:0:TCCTGAGC+TATTCCTT;FP:4250;RQ:34.83
+GTGTATAAGAGACAGAGTCAGTCTATAGGGACAGC
++
+AAAAAEEEEEEEEEEEEEEEEEAEEEEAEAEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:23937:2223___1:N:0:TCCTGAGC+TACTCCTT;FP:26022;RQ:34.63
+GAGATGGTCAGTGCAGAGTGATCGCAGCTACCGGAAAAGAGTTAGCTAGCAATGGGAGCTGAATGAGGCCGTGGT
++
+AAAAAEEEEEEEEEE6EEE6EEEAEEEEE/EEEE/E/E/EEEAE<AAEEA/EEEAAEEEAAE/AA//EEEE/AEA
+@NB500968:8:H5CFGAFXX:1:11101:25267:2246___1:N:0:TCCTGAGC+TACTCCTT;FP:4173;RQ:34.83
+CAGCCTCTATATATAGGGGCATCCNNNNNNNNNNN
++
+A/AAAAEEEEEEEEEAEEEE6EAE###########
+@NB500968:8:H5CFGAFXX:1:11101:19822:2253___1:N:0:TCCTGAGC+TACTCCTT;FP:41486;RQ:34.63
+GGCTTCTTACCCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCGGCCCCAAGACCTCTAATCATTCGCTTTA
++
+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEAEEEEEEEE<
+@NB500968:8:H5CFGAFXX:1:11101:6670:2262___1:N:0:TCCTGAGC+TACTCCTT;FP:14539;RQ:3.28
+CCTCTATAACCGCATCGGAGACATCGGATTCATTTTAGCTATAGTTTGATTTTCCCTAAACATAAGCTCATGAGA
++
+AAAA/////EEEE///EE/E/<//E<EE/////<AE/AA<A6<EE/EE6<<E////<///</////A/E//AAE/
+@NB500968:8:H5CFGAFXX:1:11101:14858:2304___1:N:0:TCCTGAGC+TACTCCTT;FP:4202;RQ:34.63
+GCACTGCAGAGGTCTCTAGACAGCACTCTCTTCCTCTCCATGGTCCTCTCTTCCTCCAGCGCTGAGTACTGCTG
++
+AAAAAEEAEEEEEEEEEE6/E/EE/EEEEEEEE/EEEEEEEEEAEEEE/EEEEEEEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:10463:2345___1:N:0:GCCTGAGC+TACTCCTT;FP:24337;RQ:34.81
+GTGTATAAGAGACAGCGTCTCTGTATAGGGGAAGT
++
+AAAA6E66E/E/A/A/E//EE//A///EEEE///E
+@NB500968:8:H5CFGAFXX:1:11101:24405:2351___1:N:0:TCCTGAGC+TACTCCTT;FP:5580;RQ:35.18
+GTATAAGAGACAGACCCTATATATAGGGTCNNNNN
++
+AAAAAEEEEEEEEEEEEEEEAEAEEEEEEE#####
+@NB500968:8:H5CFGAFXX:1:11101:6508:2370___1:N:0:TCCTGAGC+TACTCCTT;FP:64422;RQ:34.83
+CAGCTAGACGGTATAGGGCTGGGCNNNNNNNNNNN
++
+A/AAA/E/EEE//A/EEEAAEEEA###########
+@NB500968:8:H5CFGAFXX:1:11101:21730:2488___1:N:0:TCGTGAGC+TACTCCTT;FP:57284;RQ:35.18
+ATACACATCTGACGCTCCCTATATGTAAAGANNNN
++
+AA/A///E</E/EEAE////////AEA//E/####
+@NB500968:8:H5CFGAFXX:1:11101:5499:2506___1:N:0:TCCTGAGC+TACTCCTT;FP:0;RQ:1.56
+GATTTGCCTTATAGGGAGCGTCAGATTTGTATAAGAGACAGCTCCACCCTATACTCGCCGC
++
+A//A<<AA<////A/E/E6<///A//////////////6///<//////////////////
+@NB500968:8:H5CFGAFXX:1:11101:20021:2516___1:N:0:TCCTGGGC+TACTCCTT;FP:28351;RQ:34.83
+CTAAAAGAGACAGTGCACGACTTAAGGGGATGAGTGTCTCGTATACACGTCTGACGCTCCCGCTATAAAACCACG
++
+//////E/E/E/E////EE/EE///E/EA//////E/E/////E/A///A//E/<<////A/E<///////E//E
+@NB500968:8:H5CFGAFXX:1:11101:4827:2579___1:N:0:TCCTGAGC+TACTCCTT;FP:8626;RQ:34.83
+ATGTGTATAAGAGACAGTCGAAGTCTATAGGGATTCC
++
+AAAAAE/EA/E/E///E/A/A/EE//EE/E/E/E/AE
+@NB500968:8:H5CFGAFXX:1:11101:25015:2619___1:N:0:TCCTGAGC+TACTCCTT;FP:0;RQ:1.56
+GCGTCAGATGTGAGCGTCAGATGTGTATAAGAGACAGGATCCCCCTATAGCAAGAAG
++
+AAAA/AAA<//A/E6A6EEE//A/6//</A6E/AE6/EE<EAEEEA</AEE///AAE
+@NB500968:8:H5CFGAFXX:1:11101:11758:2662___1:N:0:TCCTGAGC+TACTCCTT;FP:32308;RQ:34.83
+GTGTATAAGAGACAGGTTCGGTCTATAGGGCATCT
++
+AAAAAEEEEEEEEEEEEEEEEEEEEEAEAEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:25905:2738___1:N:0:TCCTGAGC+TACTCCTT;FP:0;RQ:1.56
+GTCAGATGTGTATAAGAGACAGTGTGTGAGTATAGGGAAACC
++
+/AAAAEE/AEAA////AEAA/AAEE/EEEAE/E//AE/E/EA
+@NB500968:8:H5CFGAFXX:1:11101:23131:2747___1:N:0:TCCTGAGC+TACTCCTT;FP:61606;RQ:34.61
+GAATAAGAGACAGATGAGCAGTATAGGGAGCATATTATTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACTAA
++
+A/AAAEEEEAEEAE<EEEEE/E6E///EE/EEE/6//6E/EEAAEEE/EAE<E//6AAAEEE/EE6AEE6EE//A
+@NB500968:8:H5CFGAFXX:1:11101:20373:2751___1:N:0:TCCTGATC+TACTCCTT;FP:283;RQ:34.93
+GATGTGTATAAGAGACAGGATCACTTTATAGGCCN
++
+AAAAAEE6AAEE/E<EEEEAE/E6</A/EAEA/E#
+@NB500968:8:H5CFGAFXX:1:11101:21204:2795___1:N:0:TCCTGAGC+TACTCCTT;FP:16279;RQ:34.81
+CAGTTAGGGCCTATAGGGTGGAGCNNNNNNNNNNN
++
+AAA<AEEEEEEEEEEEEEEEEEEE###########
+@NB500968:8:H5CFGAFXX:1:11101:10694:2817___1:N:0:TCCTGAGC+TACTCCTT;FP:10036;RQ:34.83
+NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
++
+###################################
+@NB500968:8:H5CFGAFXX:1:11101:8407:2853___1:N:0:TCCTGAGC+TACTCCTT;FP:21171;RQ:34.62
+CACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGGATGGGGTTCAACGGGT
++
+AAA6AEEEEEEE/EE/EEEEEEEEEEEEEEA6EEEEEEEE<EEEE</EEEAEEEEAEEEEEEEEAEEEE/EAEEA
+@NB500968:8:H5CFGAFXX:1:11101:17699:2880___1:N:0:TCCTGAGC+TACTCCTT;FP:52710;RQ:34.62
+CATCTGACGCAGCCGACGAAAAAACATGTGTAGATCTCTGACTTCTCAGTATCATTAAATAAAAAAAATTGATGG
++
+AAAAAEEEEE/EEEEEEE6EEA//<<EEEE//E//EEEAE///E/6///E/EA/EA//////66A//<6A///AA
+@NB500968:8:H5CFGAFXX:1:11101:2261:2941___1:N:0:TCCTGAGC+TACTCCTT;FP:2596;RQ:34.63
+CTCTTATACACATCTGATGCTGCCCCCTATATGCTCCTT
++
+A/AAAEAEE/E/AEEEEEEEEEEEEEE/E/A<EEEEE//
+@NB500968:8:H5CFGAFXX:1:11101:11672:2944___1:N:0:TCCTGAGC+AACTCCTT;FP:32501;RQ:34.63
+AAAACCCTAACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
++
+A/AA<//6/6//////66666666/6///AEE//EE/</AE</E/<EEEAEEEEEA////<EEE/E<E<A///<6
+@NB500968:8:H5CFGAFXX:1:11101:15968:2945___1:N:0:TCCTGAGC+TACTCCTT;FP:8706;RQ:34.63
+GATGTGGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCCGCAGCGCCAGTTCTGCTTACCAAAAGT
++
+AAAA/EEEEEEEEEAEEEEEEE/EEEEAEEEEEEEEEEEEEAEEE<<EEA<AEE//AAA<EAA/EEEEEEEEEE<
+@NB500968:8:H5CFGAFXX:1:11101:25690:2959___1:N:0:TCCTGCGC+TACTCCTT;FP:27004;RQ:34.81
+GTGTATAAGAGACAGTCCGAAGCTATAGGGGCGGC
++
+A/AA/EEEEEE/6EEE//E//6EEEAAE/EE/EEE
+@NB500968:8:H5CFGAFXX:1:11101:8090:2972___1:N:0:TCCTGAGC+TACTCCTT;FP:12902;RQ:34.81
+GGCATAAATTATAGGGGGTCCCNNNNNNNNNNNNN
++
+AAAAAEEEEEEEE6EAEEEEAE#############
+@NB500968:8:H5CFGAFXX:1:11101:22125:3141___1:N:0:TCCTGAGC+TACTCCTT;FP:65197;RQ:35.07
+NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
++
+###################################
+@NB500968:8:H5CFGAFXX:1:11101:6707:3162___1:N:0:TCCTGAGC+TACTCCTT;FP:0;RQ:1.56
+GCGTAAGAAAAATATAAAACACACTTAAACTCAATAAGTCCATAAATACTATTAAACACAAAAAAAGCATCCAAA
++
+///A/A/E</</EA<///A//<//E/EE//6EA/<//A/</6E//6//<///<//A6<<A/AA//////////</
+@NB500968:8:H5CFGAFXX:1:11101:24232:3202___1:N:0:TCCTGAGC+TACTCCTT;FP:19483;RQ:34.63
+TGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAG
++
+AA/AAE/AA/E6/E/EEA<AAEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEA6AAEE/E/EEEEA<EAEE
+@NB500968:8:H5CFGAFXX:1:11101:16936:3211___1:N:0:TCCTGAGC+TACTCCTT;FP:7401;RQ:34.83
+CAGTTCACTTTTATAGGGACGCACNNNNNNNNNNN
++
+AAAAAEEEEEEEEEEEEEEEEEEE###########
+@NB500968:8:H5CFGAFXX:1:11101:6680:3212___1:N:0:TCCTGAGC+TACTCCTT;FP:62506;RQ:34.81
+NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
++
+###################################
+@NB500968:8:H5CFGAFXX:1:11101:6132:3257___1:N:0:TCCTGAGC+TACTCCTT;FP:7557;RQ:34.62
+GTATAAGAGACAGGAGGCGTGTATAGGGGAATGCN
++
+AAAAAEEEEEEEEEEEEEEEEEEEAEEEE6EEEE#
+@NB500968:8:H5CFGAFXX:1:11101:3840:3285___1:N:0:TCCTGAGC+TACTCCTT;FP:16156;RQ:34.93
+CAATAGGGTATACCTGTCACTAATACACATCAGACGCTCCCCCCTATATAGTAAAT
++
+A/AAAE//EE/EEAE//E/EE/EEEEEEEEE/EEEAEEEE<A<A/</AE<EEAE<6
+@NB500968:8:H5CFGAFXX:1:11101:2858:3312___1:N:0:TCCTGAGC+TACTCCTT;FP:14452;RQ:35.12
+CTCTTATACACATCTGACGTTCCCTATATGAGTCAT
++
+/AAAAEEAEEEEEEEEEEEEEEEEEAEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:3641:3398___1:N:0:TCCTGAGC+TACTCCTT;FP:36051;RQ:35.14
+CTCTTATACACATCTGACGCTGCCCCTATAATGATATC
++
+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:20540:3401___1:N:0:TCCTGAGC+TACTCCTT;FP:48603;RQ:34.81
+CAGTTAGGGCAAAAAGGGTTGATNNNNNNNNNNNN
++
+AAAA//EEAE6/E//AAA//EEE############
+@NB500968:8:H5CFGAFXX:1:11101:19438:3463___1:N:0:TCCTGAGC+TACTCCTT;FP:0;RQ:1.56
+GCGTCGAGATGTGTATAAGAGACAGCCGAGCTCTATAGGGCAAGT
++
+AAAAAEEEEEEEEEEE<AEEEEEEEEEEEEEEEEEEEEEAEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:4772:3477___1:N:0:TCCTGAGC+AACTCCTT;FP:61788;RQ:3.33
+GTTTATAATAGACACATAACAATAAAAGAGTAACAATCACAAAAAAAAATAAGACTCTCACGACGAAACACCTTA
++
+A//A/E//E/A/E/A/E//A<E/////E/E//AE////E/AE</////EEA/E/A/E//AE/E///<<///A<A/
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/with_b_BC_AAC.fq	Mon May 09 04:08:01 2016 -0400
@@ -0,0 +1,24 @@
+@NB500968:8:H5CFGAFXX:1:11101:19474:1422 1:N:0:TCCTGAGC+TACTCCTT;RQ:1.20
+ACTACTATAGGGGGAGCGTCAGATGTGTATAAGAGACAGGTACCC
++
+AAEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:24842:1887 1:N:0:TCCTGAGC+TACTCCTT;RQ:1.02
+CTATATATAGGGGGGAGCGTCAGATGTGTATAAGAG
++
+AAEEAEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:16709:1904 1:N:0:TCCTGAGC+TACTCCTT;RQ:1.18
+GAGCTTATAGGGGTTCCATGCGTCAATGTTTCTTCTGGATACAGCAGGCCTGTTCCCGGGAGTCAATGC
++
+AAEEEEEEEEEAEEEEEAEEEEEEEEEEAEEEEEEEEEEEAEE6<EEEEEAAA<EAEEEEEEEEEEE<E
+@NB500968:8:H5CFGAFXX:1:11101:5814:2162 1:N:0:TCCTGAGC+TACTCCTT;RQ:1.18
+GCTCTTATAGGGGCCCTTGGCTTCACCACACTTTCTACTGGAAATGTGCCTTTTCCTCCATTGCTGCTAC
++
+AAEEEA/EE/E/EEEEEEEEEEEEEEEAAEEEAEEEEEA/EEEEEE<EEEAEAAEEAEEE<AAEAEEAAE
+@NB500968:8:H5CFGAFXX:1:11101:24405:2351 1:N:0:TCCTGAGC+TACTCCTT;RQ:1.21
+CTATATATAGGGTCTGTCTCTTATACCNNNNN
++
+AAEEEAEEEEEEEEEEEEAEEEEEEEE#####
+@NB500968:8:H5CFGAFXX:1:11101:20373:2751 1:N:0:TCCTGATC+TACTCCTT;RQ:1.15
+CACGTTATAGGGGGAGCGTCAGATGTGTATAAGAGACAGCCCTATACAGTGATC
++
+AAEEEEEEEEEEEEEEEEEEE<EEEEAEAEEEEEEEEEEEEEE/EEEEEE<EAA
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/with_b_BC_GTA.fq	Mon May 09 04:08:01 2016 -0400
@@ -0,0 +1,16 @@
+@NB500968:8:H5CFGAFXX:1:11101:11473:1333 1:N:0:TCCTGAGC+TACTCCTT;RQ:1.18
+ACTCGTATAGGGGACAGCGTCAGATGTGTATAAGAGACAGGGTATC
++
+AAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:12411:1441 1:N:0:TCCTGAGC+TACTCCTT;RQ:11.24
+TTCCTTATAGGGGGGAGCGTCAGATGTGTATAAGAGACAGTCTCCCTATATTTTGGC
++
+AA//AEEEEEEE//AEEE/EAEAEE/AEAE<EEEEAE/EAE<E/E</EEEE/6E/EE
+@NB500968:8:H5CFGAFXX:1:11101:19922:2125 1:N:0:TCCTGAGC+TACTCCTT;RQ:11.21
+TAAGAGACAGCGATACTTTATAGGGGGGCCGTGTGCAGACCCGCGTGTGGCGCAGGCAAGGACCCTGAAAAT
++
+AAEE/EAEEEEEEEEEEEEEEEE/EEEEEEEEAEEEEEEEEEEEEAEEA<<EEEEEEEEEEEEEEEEEEEA<
+@NB500968:8:H5CFGAFXX:1:11101:4099:3187 1:N:0:TCCTGAGC+TACTCCTT;RQ:11.21
+TAAGAGACAGCCAGCCTCTATAGGGGTGCTTGCAGGGGAGGGAAAAGGGGGAAAGCGGGCAACCAGTTTCC
++
+AAAEAE6E6EEEEEAEEA<EEEEEEEEEEEEEEEEEEEEEEEEEEAEE/EEAEEEEEEEEEEEEEAE/E//
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/with_b_un.fq	Mon May 09 04:08:01 2016 -0400
@@ -0,0 +1,360 @@
+@NB500968:8:H5CFGAFXX:1:11101:15939:1071 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.00
+CCTAAGAGTATAGGGGGCACGTCAGATGAGTATAAGAGACAGCAAATAGGTATAGGGGGCACGTCAGATGAGTAA
++
+AAAAA6/AEEEEEEEEAEEEEEEA///A/EAEEE/EAEEEEEEEEEEAEEE//E/AA/E6AE/EE/<E////A//
+@NB500968:8:H5CFGAFXX:1:11101:1121:1139 1:N:0:TCGTGAGC+TACTCCTT;RQ:0.00
+CGTAGTCATATAGGGGGAGCGTCAGATGTGTATAAGAGACAGGTATTCCCTATAGTCTACATC
++
+AAAAAEEEEAEE<EEEEEEEEEEEEEEEEEAAEEEEAEEEAEEE/EEE/EE<AAEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:7529:1175 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.02
+CAATTATGTATAGGGAGCGTCAGATGTGTATAAGAGACAGCAACCCTAAAAATATCCA
++
+AAAAAEA/EEEEEEEEEEE/EEEEEEA/EA<EEEE/AEEEE//E//6A/AE/A/AAE/
+@NB500968:8:H5CFGAFXX:1:11101:5052:1198 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.02
+GCCTGATTTATAGGGGGTTTTTAGTGGCATTCATTGTGTGGATTCCTCTGTTGCTGCCCCTGGGCTAACAAGGGT
++
+AAAAAEEEEEEEE<AEEEEEEE6EEEEAEEEEEEEEEEEAEEEEEEEEEEEEEAAE/AEEEEEAAAEEEE/EAEE
+@NB500968:8:H5CFGAFXX:1:11101:15805:1205 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.02
+GGTCTTTGTATAGGGGAGGGCCAGGCAGCTCAAAGCCTGAAGGAGGTACTTCAAGGAGGGTCCAGAGAAGCTGGG
++
+AAAAAEEEEEEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEE<EAAEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:11834:1217 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.00
+TCTCTCGTTATAGGGAGCGTCAGATGTGTATAAGAGACAGGATTCCCCTATATAGCGACCC
++
+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEEEAEEEEEEEEEA<EAEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:20105:1230 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.75
+AAGATCGCTATAGGGGTTGTTTCATGTTGCAATTTTTAGGTTACTTTTGAGTTGCTTAGGAATTGTTTTATTTAA
++
+6AAAA//EEEAEAEEEEEEEEEEEE/E/AEEEEE<E</EEAEEEEEEAAEAEEEAAE//EA//</E6EAAEA/6/
+@NB500968:8:H5CFGAFXX:1:11101:14524:1264 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.02
+ACTTAAACTATAAGTCCCAACTTAATATTATAAAATTATCATAAAAACAACTACGAGCCCACGATACAACTGATA
++
+AAAAA//EEE<EEEA//EEEE<///A/AAA/A////E/<///E/6/E/E/E6///E//6E<//6/AA//E//A//
+@NB500968:8:H5CFGAFXX:1:11101:14981:1265 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.02
+CGCGTCTCTATAGGGGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCG
++
+AAAAAEEEEEEE<EAEEEEEEEEEEEEEAEEEEEEEEEAAEEEEEEEA<EEEEEEEEE6/E66A<A/<AA<A//6
+@NB500968:8:H5CFGAFXX:1:11101:5538:1286 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.02
+ACATTCCTTATAGGGAGTGAACTAACTAGAAAACTTCTAACTAAAAGAATTACAGCTAGAAACCCCGAAACCAAA
++
+6AAAAEEEEE<E</<AAEEEEEEEEAE/AEEEEEEEAEEEEE/AE6/EEA<E/EA6EAAEEE6//6<<EEA/<<A
+@NB500968:8:H5CFGAFXX:1:11101:2141:1369 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.83
+NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
++
+################################
+@NB500968:8:H5CFGAFXX:1:11101:24482:1380 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.80
+GTGTATAAGAGACAGGCCTAATATATAGGGGGCGCCAGTCCTCCGATTGACTGAGTCGCCCGGGTACCCGTGTAT
++
+AAAAAEE/E/E/<EEEE/EAEE<EEEEEEEEEEEEEAEEA/E/EAEEEEEEEEEEEEEEE<E/EE<<6EEAEEEE
+@NB500968:8:H5CFGAFXX:1:11101:2671:1393 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.02
+GGTCCCCTATAAGGGGATTCTGTCTCTTATACCNN
++
+///AA6E/6AA///EAE//E6E6E6E/6AAE/E##
+@NB500968:8:H5CFGAFXX:1:11101:5889:1458 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.01
+TAGTAGAGTATAGGGGAGCGTCAGATGTGTATAAGAGACAGCGTATCCCTATACCCCTTCGCTG
++
+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:8662:1469 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.85
+TTAGGTCTTATAGGGGGAGCGTCAGATGTGTATAAGAGACAGGTTATC
++
+AAAAAEEEEEEEEEEEEEEEEEEEEEEEAEAE<AEAEEEEEEEEEE<E
+@NB500968:8:H5CFGAFXX:1:11101:9851:1471 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.01
+GGGCCCTATAACGGGCCCCTGTCTCTTATACCNNN
++
+AAAA6EEEEEEEAEEEEEEEA/AA/EEEEE//###
+@NB500968:8:H5CFGAFXX:1:11101:7850:1487 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.02
+ATTCCAATATAGGGGACCCACATCTTCCTCTGCTTCTAGATGAAGGATGTATTCTTCTTCCTCTTCTTCCTCTG
++
+AAAAAEEE/EEE/EEEEEEAAEEE/EEEEEAEEEEEEEEEEEEEEAEAE6EEA<EEEAEE<6E/<EE<<E6E/<
+@NB500968:8:H5CFGAFXX:1:11101:5425:1488 1:N:0:TCCTGAGC+TACTCCTT;RQ:1.00
+ATCACATGTATAGGGGGAGCGTCAGATGTGTATAAGAGACAGACTCCCCTATATGGACACA
++
+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:21998:1542 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.01
+ATGTCATATATAGGGGGCGTCAGATGTGTATAAGAGACAGGG
++
+AAAAA6EEEEEEEEEEEEEEEEAEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:2814:1550 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.83
+NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
++
+################################
+@NB500968:8:H5CFGAFXX:1:11101:8024:1581 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.02
+GCCCGGCTTATAGGGGTGACCCGCCGCTTCAGGGACCCGCTTCTCTCATCTTCCCCAAAGTTCTGGACAGATGAT
++
+AAAAAEEEA/EE/A/EEEEEEEAEEEEEAEAEEEEEEEEEEAEEEEAEEEAEAEEEEEEEEAEEEEEEEEEE/A<
+@NB500968:8:H5CFGAFXX:1:11101:8843:1628 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.02
+GGTCGGCTTATAGGGGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGG
++
+AA/AAEEEEEEE<E/EEEEEEEEEAEAEEEEEEEEEEE6/AEEEEAAEE6EA<EEEEE<EEEEEEEEE/AAAAEA
+@NB500968:8:H5CFGAFXX:1:11101:23474:1673 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.76
+AAGTGCGATATAGGGAGCGTCAGATGTGTATAAGAGACAGAGGCGCCCTATAGAATATAT
++
+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEE/EEEEE
+@NB500968:8:H5CFGAFXX:1:11101:13010:1719 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.75
+AAGACAGTGTGATTATATAGGGGACAGCGTCAGATGTGTATAAGAGACAGCTAAGCCCTATACGTTTTTTC
++
+/A/AAEAAEEEE/E/EAEAAEE</EEEEEEAEEEEAAEAAAEAEEEAEEEEE/EE/EEE6A/EEEAEEEEA
+@NB500968:8:H5CFGAFXX:1:11101:15935:1725 1:N:0:GCCTGAGC+TACTCCTT;RQ:0.80
+GTGTATAAGAAACAGGCTTTATATAGGGGCACCGTCAGATGTGTATAAGAGACAGTTGTCCCCTATACCACGC
++
+/AAAAEEEEAEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE/E<<EEEEE/EE<EEEE/AEEEEEEEAEEE
+@NB500968:8:H5CFGAFXX:1:11101:1482:1752 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.02
+CCAAATACTATAGGGGGGAGCGTCAGATGTGTATAAGAGACAGTTTTACCC
++
+AAAAAEEEAA/AEEEE/EEEEEEEEEE</EEEEEEEEEEEEEEEEE<EAEA
+@NB500968:8:H5CFGAFXX:1:11101:21720:1786 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.02
+ATTTGAACTATAGGGGGGAGCGTCAGATGTGTATAAGAGAC
++
+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:9012:1809 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.02
+ACAACACTTATAGGGGGGAGCGTCAGATGTGTATAAGAGACAGGTACCCCCTATATCATAGGC
++
+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:12567:1818 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.85
+TTAAACTTTATAGGGGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGG
++
+AAAAAEEEEEEE<E/EEEEE/EA/EEEAEEEEEA/EEAEEEEE/EEEA/EEAEAEE/A<E/EEEAA<<A6AA<//
+@NB500968:8:H5CFGAFXX:1:11101:20110:1819 1:N:0:TCCTGAGC+TAATCCTT;RQ:0.02
+TTTAAGTTTATAGGGGGATTCTTGAAGTATAACAGGAGAATCAAATAATTAAAGAGGGGGCTGGAGAGATAGCT
++
+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:12108:1890 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.02
+CAGTTAGGTGTTATAGGGATCCCAAGACAAGGTTTCTCTGTGTCTATCCCTGGCTGTCCTTGAACTTCGGCAT
++
+6AAAA66EAE6EEEEEAE/EAAEEE/EEEEAEEEEEE/EEEEE/EAEEE/E/EAEEE<<EE/EAEEE6/EEE<
+@NB500968:8:H5CFGAFXX:1:11101:12189:1919 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.02
+TATTTCCATATAGGGACACCCTTTATAGTGATGGATTGGATCTGTTTGAAGGCAAAGTCCTTCCCAGACTTATCA
++
+AAAAA/EEEA6<///EEEEEEE/EEE/EEAEEEEEEEEEEE/EE<EAAAE/E<</EA///A</E/A/AAA<A/</
+@NB500968:8:H5CFGAFXX:1:11101:26750:1960 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.76
+AAAGCAAGTATAGGGGGGAGCGTCAGATGTGTATAAGAGACAGCTGACCC
++
+AAAAAAEEE/<AEEAEEEAEEEAEEAEAEAEE/EEEEAEAAEEEEE/EEE
+@NB500968:8:H5CFGAFXX:1:11101:21600:1964 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.03
+GCCATGGTTATAAGGGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAA
++
+AAAAAEEAEEEE/E/EEEEEEAEE/AAAEEEEEEEEEEEEEE<EEEAEEEEAAAEAAEEEE<EA<A<EA<<E<E
+@NB500968:8:H5CFGAFXX:1:11101:6961:1984 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.75
+GGATGTCGCTATAGGGCGCCGCCGCCTCCTCCTCCTCTCTCGCGCTCTCTGTCCCGCCTGGTCCTGTCCCACCC
++
+AAAAAAEEEEEEEEEEEEEAEAAEEE</EEEE/EEEEEEAEEEAAEEEEEEEEEEEEEEAEEEAEEEEAE/EEE
+@NB500968:8:H5CFGAFXX:1:11101:20646:1998 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.00
+CCGCAAGTGCGTTCGAAGTGTCGATGATCAATGTGTCCTGCAATTCACATTAATTCTCGCAGCTAGCTGCGTT
++
+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEAEEEEEEAEEEEEEEEEEEEEEEEAAEE
+@NB500968:8:H5CFGAFXX:1:11101:11625:2006 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.81
+AATTGTTGTATAGGGGACAGCGTCAGATGTGTATAAGAGACAGCAACCACC
++
+AAAA/AAA/EEEEEEAEA66E/<AEAEE/E/EEAEEEEAEEEEEAEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:10474:2017 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.02
+CAAACTGGTATAGGGGGAGCGTCAGATGTGTATAAGAGACAGTCGTCC
++
+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:17381:2018 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.02
+TTTATTAGTATAGGGAACGTCAGATGTGTATAAGN
++
+AAAAA/EAEEEE<EEEEE/EEEAEEEEEAE</EA#
+@NB500968:8:H5CFGAFXX:1:11101:9520:2027 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.00
+TCTTCACTTATAGGGAGCGTCAGATGTGTATAAGAGACAGCTACATCCCTATAAGGATGTAC
++
+AAAAAEEEEEEEEEAEEEEEEEEEEEEEEEEAEEEEEEEEEEAEEEEEEE/EEEEE<AEEEA
+@NB500968:8:H5CFGAFXX:1:11101:23302:2031 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.02
+AGATCCTTTATAGGGAGCGTCAGATGTGTATAAGAGACAGATCGTCCCTATAGCTCCAATC
++
+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEAEEEEEEAEE
+@NB500968:8:H5CFGAFXX:1:11101:19677:2089 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.02
+ATGCGTGATATAGGGGGCGCCAGTCCTCCGATTGACTGAGTCGCCCGGGTACCCGTGTATCCAATAAACCCTCTT
++
+/AAAAEAEEEEE<EEEEEEEEEAEEEEEEEAE/EEEEEEEEAEEE//E/EEE/A<AAAAAA<AEAE/EE/AAA<6
+@NB500968:8:H5CFGAFXX:1:11101:26158:2095 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.00
+CCTCATTATATAGGGGGGAGCGTCAGATGTGTATAAGAGACAGCCACTCCCTATAGGATTTCC
++
+AAAAAAEEEEEEEEEEEEEEEEEEAEEEEEEEEEEEEEEEEEEA<EEEEAEEA<6EEEEE<EA
+@NB500968:8:H5CFGAFXX:1:11101:7883:2101 1:N:0:TCCGGAGC+TACTCCTT;RQ:0.00
+TGGACGTCTATAGGGGGAGCGTCAGATGTGTATAAGAGACAGCATCACCCTATAGGCTGTCT
++
+AAA6AEEEEE/EEEEEEEEEEEEEEEEEEAEAEEEEEEEEEEEEEEEEEEEEE<EEEEEEAE
+@NB500968:8:H5CFGAFXX:1:11101:24496:2131 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.77
+AAAATTCTTATAGGGGGGAGCGTCAGATGTGTATAAG
++
+/AAAAEEEEAE/EEAEEEEEEEEEEE/EEEEEEE/AE
+@NB500968:8:H5CFGAFXX:1:11101:5796:2184 1:N:0:TCCTGAGC+TATTCCTT;RQ:0.02
+ACAAGCGGTATAGGGGGAGCGTCAGATGTGTATAAGAGACAGGCTGTCCCTATAGACTGACT
++
+AAAAAEEEEEAEAEEEEEEEEEEEEEEEEEAEAEEEEEEEEEEEEEEEEAEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:23937:2223 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.02
+CGCCGGCGTATAGGGGCTTCTTCTGAAGAGCTCCACCTGTGGTCTCTCCACCTCTCGAGGCTCTTCACTAGCCT
++
+6AAAAAEEAE6EEA/EEEEAEA/AEEEEEEEA/</</</A//EAEEE<AE66A666/A/E6E/AAAAA////E/
+@NB500968:8:H5CFGAFXX:1:11101:25267:2246 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.02
+ACAACATCTATAGGGGAGCGTCAGATGTGTATAAGAGACAGGATGCCCCTATATATAGAGGCTG
++
+AAAAAAEEEEAEAEEE6EEEEAAEEEEEEEAEEEEEEEEEEEEEEE6EEEEEEEEEAEAEEAEA
+@NB500968:8:H5CFGAFXX:1:11101:19822:2253 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.75
+GGAGAATGTATAGGGGACGTGCAAATCGGTCGTCCGACCTGGGTATAGGGGCGAAAGACTAATCGAACCATCTAG
++
+AA6AAEEEEEEEEAEEEEEEEEEEEEEEAEEEEEEEEEEEEAEEEEEAEAEE/EEEEEEEEE<<EEEEAEEEA//
+@NB500968:8:H5CFGAFXX:1:11101:6670:2262 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.02
+ATGATAGTTATGGGTGGAGGCCAAATTGTGCTGATTTTCCTGTAGCTGCGATTAATAGGCCTATAAGTGGAATTA
++
+/AAAAEEEEAAE<EEEEEEEEEEEEAEAEEAEEEEEEEE/EAEEEAEEAEEAE/EEAE/</E/EA66A<//<EE/
+@NB500968:8:H5CFGAFXX:1:11101:14935:2297 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.02
+GATGTGCATAAAAGACAGATAGTACGTATAGGGGGGAGCGTCAGATGTTTATAAGAGACAGAATCGCCCTATAG
++
+AAAAAAAEEAEEEEEEEEEEEEEEEEEEEEEEEAEEEEEAE/A/EEAE/E/6/E///E/E/</E///A/AA/</
+@NB500968:8:H5CFGAFXX:1:11101:14858:2304 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.02
+ACAACGGGTATAGGGGTCTCCGGCTTGTGTAGACTCTGCCTGGAAGTGAAGTTGTGGACTCCCGACCTCCAGTC
++
+AAAAAEEEEEEEEE/EEEEEEEEEEEEEEEEEEEEEA/E/AEEEEEAEEEEAE/<AAEEE<EAEEE<EEAEEEE
+@NB500968:8:H5CFGAFXX:1:11101:10463:2345 1:N:0:GCCTGAGC+TACTCCTT;RQ:0.00
+CCTTACACTATAGGGTCCAGCGTCAGATGTGTATAAGAGACAGAGTTCCCCTATAGAGAGACG
++
+AAAAAEEEEEEEEEEEEEEEEEEEEE6EEEEA/A//E/EEEEEEEEEEEEEEAA/AAEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:6508:2370 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.02
+TTGTGGCGTATAGGGGGAGCGTCAGATGTGTATAAGAGACAGCCCAGCCCTATACCGTCTAGCTG
++
+AAAAAEEEEEEEEEEEEEEEEEEEEEEEAEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:13250:2472 1:N:0:TCCTGAGC+TACTCCTT;RQ:1.00
+GTCTCCGCCCTTGCTGACTTTCCATCATTTTTAAGATCAGGTGGAACTGGAGCCTCTGCCTGTGGCTGATCTGAG
++
+AAAAAEAA/EAA<EEEEE/EEAAEEAE<EEA/E//EE/E/EEAAEEEEA6A/6EAA6/6<6/<//A/<AAE6AA/
+@NB500968:8:H5CFGAFXX:1:11101:21730:2488 1:N:0:TCGTGAGC+TACTCCTT;RQ:0.00
+TCTTTACATATAGGGAGCGTCAGATGTGTATCNNN
++
+AAAAAEAEEE<EEEEEEEEEEEEEEEEEAEAE###
+@NB500968:8:H5CFGAFXX:1:11101:5499:2506 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.83
+NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
++
+################################
+@NB500968:8:H5CFGAFXX:1:11101:20021:2516 1:N:0:TCCTGGGC+TACTCCTT;RQ:0.00
+CGTGGTTTTATAGGGGGAGCGTCAGATGAGTATAAGAGACAGTCATCCCCTATAGTCGTTCA
++
+AAA66E/EEEEAEEEEAEEAAEEAAE///E/EAAE/EAEE//EEE/EEEEEE/EE/EA6EEE
+@NB500968:8:H5CFGAFXX:1:11101:4827:2579 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.18
+AGACGTAGTATAGGGGACAGCGTCAGATGTGTATAAGAGACAGGGAATCCCTATAGACTTTGC
++
+AAAAAEEE6EAEEEEEEEEEEEEE/E<AAAEEEEEEEE/E/EEEEEEEEEEEAEEAEAAEEE/
+@NB500968:8:H5CFGAFXX:1:11101:25015:2619 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.83
+NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
++
+################################
+@NB500968:8:H5CFGAFXX:1:11101:11758:2662 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.02
+CTTGATCATATAGGGGACAGCGTCAGATGTGTATAAGAGACAGAGATGCCCTATAGACCGAAC
++
+AAAAAAEEEEEEE/EEEEEEE/EAE/EEE/EEEAEE6E6EEEEEAAEA/E/AEEEA/E6EA/E
+@NB500968:8:H5CFGAFXX:1:11101:25905:2738 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.83
+NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
++
+################################
+@NB500968:8:H5CFGAFXX:1:11101:23131:2747 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.85
+TTAAGGCGTATAGGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCGAGGTTATCTA
++
+AAAAAEAEEEEEE<AEEEEEEAEAEEEE/EEEAEEEEA/EEEEAEA/EA<EAAAEEAE<AE/A<<EEEA<<EEA<
+@NB500968:8:H5CFGAFXX:1:11101:8485:2792 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.00
+CGTCAGATGTGGTCCCGCTCACCATGCCGCTGCTGTAGCCGCTCACCAACCTCCTGTGCCCCCCGCAACTCAT
++
+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEE6EEEEEEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:21204:2795 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.02
+ATTTGCCTTATAGGGAGCGTCAGATGTGTATAAGAGACAGCTCCACCCTATAGGCCCTAACTG
++
+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:10694:2817 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.95
+AGCTATCATATAGGGGGAGCGTCAGATGTGTATAAGAGACAGCCCTTCCCTATACGCTTGGCC
++
+AAAAAEAEEEEEEEEEEEEEEEEEEEEE<EEEEAEEEEEEEEEE/A<E<EEEA/EEEAEEEE/
+@NB500968:8:H5CFGAFXX:1:11101:8407:2853 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.02
+CCAGGTATTATAGGGAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTT
++
+6AAAAEAEE/AE//</EEEEEEAEEEEEAEEEEEEEEEAEAEEAAEEEE/EEEAAAEA//EEEAEAAA/EAEAE6
+@NB500968:8:H5CFGAFXX:1:11101:17699:2880 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.02
+TATCTGCGTATAGGGCTTTGGAATCTTCACAAGCAGAACCAGTCAAAACCCCGGCAAGCACAAAGAGACTCTCCA
++
+AAAAAEEEAEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE<EEEEEEEEEEE6AEAEAEEEEA
+@NB500968:8:H5CFGAFXX:1:11101:2261:2941 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.75
+AAGGAGCATATAGGGGGCAGCATCAGATGTGTATAAGAGACAGAAGGAGCAGAGAGCGGCCAGCAACCGATGAGA
++
+AAAAAEEEEEAEEEEEEEEEEEEEEEEEEAEEEAEEEEE///////EA//E/E/A/////E<EE//</E<<////
+@NB500968:8:H5CFGAFXX:1:11101:11672:2944 1:N:0:TCCTGAGC+AACTCCTT;RQ:0.02
+CTTGTTCCTATAGGGGGGTGAAATACTGTGAGGAACCCAGAAGTGAGTGGAAAGATAACTGGCTTTTTTTTTTTT
++
+AAAAAEAEEEEEE<AAEEEEEEEEEEEAAAEEEEEAA<E/EEEEEEEAAEAEEEE<EEE<</EE6AA/6/<66//
+@NB500968:8:H5CFGAFXX:1:11101:15968:2945 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.02
+AGAGAAAGTATAGGGGCAAATCGGTCGTCCGACCTGGGTATAGGGGCGAAAGGCTAATCGAACCATCTAGTAG
++
+AAAAAEEEEEEEEEEEEEEEEEAEEEEEEEEEEEEEEEEAEEEEEEAEEEEE<EEEEAAEAEEAE<AEAEEEE
+@NB500968:8:H5CFGAFXX:1:11101:25690:2959 1:N:0:TCCTGCGC+TACTCCTT;RQ:0.00
+CGGCCTTATATAGGGAGCGTCAGATGTGTATAAGAGACAGGCCTCCCCTATAGCTTCGGA
++
+AAAA6EEE//6EEA/AE6EEEEEEEEEAAEEEEEEEEEEEEEEAEE/E/EAEEEEAEEEE
+@NB500968:8:H5CFGAFXX:1:11101:8090:2972 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.80
+ATAGCGCGTATAGGGCAACGTCAGATGTGTATAAGAGACAGGGACCCCCTATAATTTATGCC
++
+AAAAAEEEEEAEEEEEEEEEEEEEEEEEEEEEAAEEEEEEEEEAEE<EEEEEAE<EEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:23310:3069 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.02
+CAGGCCAATCGTATAGGGGTTGATACTCAAGTCTACTTGTTCCCTTTTATTTAATCCTGAACCCCATCCCATGGA
++
+AAA/AEEEEEEEAE//EEE////EAA//EE/EEEEEE//EEE/E/E/EEEEEEE<A<E/AEE//AE/<AAEEEE/
+@NB500968:8:H5CFGAFXX:1:11101:9254:3110 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.00
+TGTTATAAGGCGTCAGATGTGTATAAGAGACAGGGATCCCCTATAAAGAGCCTAAC
++
+AAAAAEEEEEEEEEEEEEEEEEEEAEEEE<EAEEE/EEEEEEEEE/EEEEEEEAA/
+@NB500968:8:H5CFGAFXX:1:11101:22125:3141 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.02
+TTTGGGTCTATAGGGAGCGTCAGATGTGTATAAGAGACAGCTC
++
+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEE
+@NB500968:8:H5CFGAFXX:1:11101:6707:3162 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.83
+NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
++
+################################
+@NB500968:8:H5CFGAFXX:1:11101:24232:3202 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.02
+CATAACGTTATAGGGGTTGGTTTTCGGAACTGAGGCCATGATTAAGATGGACGGCCGGGGGCATTCGTATTGCG
++
+AAAAAEEEEEAE///EEEEEEEEEEAEEEEEEE/E<EEEEEA</EEE/A/EE<E666<EEAEEEE/6/A66/66
+@NB500968:8:H5CFGAFXX:1:11101:16936:3211 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.02
+ACTATGGCTATAGGGGAGCGTCAGATGTGTATAAGAGACAGTGCGTCCCTATAAAAGTGAACTG
++
+AAAAAEEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEEEEEEA/EEEAEAEEE
+@NB500968:8:H5CFGAFXX:1:11101:6680:3212 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.02
+TTCAAGGGTATAGGGTCCAGCGTCAGATGTGTATAAGAGACAGCTCCTCCCTATAAG
++
+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:15733:3232 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.02
+TCCCCTATATTTGGGGACTGTCTCTTATACACCNN
++
+AAAAAEEE/EEEEEEEEEEEEEEEEEEEEEEEE##
+@NB500968:8:H5CFGAFXX:1:11101:3009:3242 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.02
+GGTTAAGGCTAGCCTATTCTACATAGAGTGTTCTAGGCCTTCCAGGGCCACATAGACTCTTGTTTCAAAAAAAT
++
+A//AAEEEEEEEEEEEAEE/EEEEAAEE/AAEEEAEEEEAEEEEAEEEEEEEEEEEEEEA6EEEE<EEE/A/EA
+@NB500968:8:H5CFGAFXX:1:11101:6132:3257 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.03
+ACTCGACCTATAGGGCGTCAGATGTGTATAAGAGACAGCATTCCCCTATACACGCCTCCTGTCTCTTATAC
++
+AAAAA/EEE6E/EEEEEEEAEEEEEEEEEEEEEEAEAEEAEEEEAEEEEEEEEEEEEEEEEAE/EEEEEEA
+@NB500968:8:H5CFGAFXX:1:11101:3840:3285 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.02
+ATTTACTATATAGGGGGGAGCGTCAGATGTGTATAAGAGACAGGTATACCCTATAG
++
+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:2858:3312 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.02
+ATGACTCATATAGGGAACGTCAGATGTGTATAAGAG
++
+AAAAAE/EE/A/<AEEE/AE/E/EAAEEEEEEEAEE
+@NB500968:8:H5CFGAFXX:1:11101:3641:3398 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.15
+GATATCATTATAGGGGCAGCGTCAGATGTGTATAAGAG
++
+AAAAAEEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEE
+@NB500968:8:H5CFGAFXX:1:11101:20540:3401 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.86
+GTTCTCGTTATAGGGAGCGTCAGATGTGTATAAGAGACAGATCAACCCTATATGCCCTAACTG
++
+AAA6AE/AAEEEEEEE/E///E/E</A/EEA<E/E/EEE/E</E///A/E6<E/A/<E/EEE/
+@NB500968:8:H5CFGAFXX:1:11101:18503:3442 1:N:0:TCCTGAGC+TACTCCGT;RQ:0.01
+TAATCCCTATAAGGGATTACTGACTCTTATACATGTATCTAATACACATCTCCGAGCCCACGAGACTCCTGAGCA
++
+AAAAAE/AAEEEEEEAAE/E//////EEAA///A/E/<///AEEEE/E/EE<<///E<EEE/E/AE6/EEEE//6
+@NB500968:8:H5CFGAFXX:1:11101:19438:3463 1:N:0:TCCTGAGC+TACTCCTT;RQ:0.83
+NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
++
+################################
+@NB500968:8:H5CFGAFXX:1:11101:4772:3477 1:N:0:TCCTGAGC+AACTCCTT;RQ:0.86
+TTACCCTATACTGGGTAACTGTCTCTTATACACCN
++
+AAAAAEEEEAEEEEAEEEEEEEAEEEAEEE<EEE#
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/tool-data/all_fasta.loc.sample	Mon May 09 04:08:01 2016 -0400
@@ -0,0 +1,17 @@
+#This file lists the locations and dbkeys of all the fasta files
+
+#This file has the format (white space characters are TAB characters):
+#
+#<unique_build_id>	<dbkey>		<display_name>	<file_path>
+#
+#So, all_fasta.loc could look something like this:
+#
+#apiMel3	apiMel3	Honeybee (Apis mellifera): apiMel3		/path/to/genome/apiMel3/apiMel3.fa
+#hg19canon	hg19		Human (Homo sapiens): hg19 Canonical		/path/to/genome/hg19/hg19canon.fa
+#hg19full	hg19		Human (Homo sapiens): hg19 Full			/path/to/genome/hg19/hg19full.fa
+#
+#Your all_fasta.loc file should contain an entry for each individual
+#fasta file. So there will be multiple fasta files for each build,
+#such as with hg19 above.
+#
+
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/tool-data/tagdust_architecture.loc.sample	Mon May 09 04:08:01 2016 -0400
@@ -0,0 +1,15 @@
+#This is a location file for tagdust reference fasta files
+
+#See tagdust reference manual for architecture recommendatons
+
+#If the architecure file include a barcode HMM (B:) set the nacode field to yes
+#Otherwise leave is as no
+
+#Planemo does not handle commas in value field well so avoid these if including the file in testing.
+
+#file has this format (white space characters are TAB characters):
+#
+#value	dbkey	name	barcode	path
+#
+
+
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/tool_data_table_conf.xml.sample	Mon May 09 04:08:01 2016 -0400
@@ -0,0 +1,11 @@
+<!-- Use the file tool_data_table_conf.xml.oldlocstyle if you don't want to update your loc files as changed in revision 4550:535d276c92bc-->
+<tables>
+    <table name="all_fasta" comment_char="#">
+        <columns>value, dbkey, name, path</columns>
+        <file path="tool-data/all_fasta.loc" />
+    </table>
+    <table name="tagdust_architecture">
+        <columns>value, dbkey, name, barcode, path</columns>
+        <file path="tool-data/tagdust_architecture.loc" />
+    </table>
+ </tables>
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/tool_dependencies.xml	Mon May 09 04:08:01 2016 -0400
@@ -0,0 +1,6 @@
+<?xml version="1.0"?>
+<tool_dependency>
+    <package name="tagdust" version="2.31">
+        <repository changeset_revision="1b0267fab15a" name="package_tagdust_2_31" owner="brenninc" prior_installation_required="True" toolshed="https://toolshed.g2.bx.psu.edu" />
+    </package>
+</tool_dependency>