Mercurial > repos > clairetn > detrprok_wf
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author | clairetn |
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date | Mon, 20 May 2013 13:44:44 -0400 |
parents | c265b11e7277 |
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{ "a_galaxy_workflow": "true", "annotation": "", "format-version": "0.1", "name": "Detrprok_wf", "steps": { "0": { "annotation": "Select here annotation file containing coding genes, tRNAs, rRNAs and known ncRNAs. You can download it from GJI (http://genome.jgi.doe.gov) or NCBI (http://ftp.ncbi.nih.gov/genomes/Bacteria). Caution: 1srt column should have exactly the same identifier as the genome sequence used for mapping.", "id": 0, "input_connections": {}, "inputs": [ { "description": "Select here annotation file containing coding genes, tRNAs, rRNAs and known ncRNAs. You can download it from GJI (http://genome.jgi.doe.gov) or NCBI (http://ftp.ncbi.nih.gov/genomes/Bacteria). Caution: 1srt column should have exactly the same identifier as the genome sequence used for mapping.", "name": "genome annotations" } ], "name": "Input dataset", "outputs": [], "position": { "left": 200, "top": 231.00001525878906 }, "tool_errors": null, "tool_id": null, "tool_state": "{\"name\": \"genome annotations\"}", "tool_version": null, "type": "data_input", "user_outputs": [] }, "1": { "annotation": "Select here the bam file resulting from the mapping of RNA-seq reads on the genome sequence. You may use Bowtie or another mapper (use appropriate mapping option to retain only uniquely mapping reads).", "id": 1, "input_connections": {}, "inputs": [ { "description": "Select here the bam file resulting from the mapping of RNA-seq reads on the genome sequence. You may use Bowtie or another mapper (use appropriate mapping option to retain only uniquely mapping reads).", "name": "read alignments on genome" } ], "name": "Input dataset", "outputs": [], "position": { "left": 205, "top": 403.00001525878906 }, "tool_errors": null, "tool_id": null, "tool_state": "{\"name\": \"read alignments on genome\"}", "tool_version": null, "type": "data_input", "user_outputs": [] }, "2": { "annotation": "Select here the GFF file features that will be considered as initial annotations. Any term added here will cause pre-existing features with this term not to be predicted again by the workflow. Please use a comma separated list and use \"CDS\" rather than \"gene\" for protein coding genes. Suggested value: rRNA,tRNA,ncRNA,CDS", "id": 2, "input_connections": { "formatType|inputFileName": { "id": 0, "output_name": "output" } }, "inputs": [ { "description": "runtime parameter for tool clean transcript file", "name": "optionType" } ], "name": "clean transcript file", "outputs": [ { "name": "outputFile", "type": "gtf" } ], "position": { "left": 457, "top": 218.00001525878906 }, "post_job_actions": { "RenameDatasetActionoutputFile": { "action_arguments": { "newname": "annotation selection" }, "action_type": "RenameDatasetAction", "output_name": "outputFile" } }, "tool_errors": null, "tool_id": "toolshed.g2.bx.psu.edu/repos/yufei-luo/s_mart/CleanTranscriptFile/1.0.0", "tool_state": "{\"optionType\": \"{\\\"type\\\": \\\"Yes\\\", \\\"value\\\": {\\\"__class__\\\": \\\"RuntimeValue\\\"}, \\\"__current_case__\\\": 0}\", \"formatType\": \"{\\\"inputFileName\\\": null, \\\"__current_case__\\\": 0, \\\"FormatInputFileName\\\": \\\"gff\\\"}\", \"chromInfo\": \"\\\"/srv/galaxy/galaxy-dist/tool-data/shared/ucsc/chrom/?.len\\\"\", \"__page__\": 0}", "tool_version": "1.0.0", "type": "tool", "user_outputs": [] }, "3": { "annotation": "Technical step (format change).", "id": 3, "input_connections": { "input1": { "id": 1, "output_name": "output" } }, "inputs": [], "name": "BAM-to-SAM", "outputs": [ { "name": "output1", "type": "sam" } ], "position": { "left": 428, "top": 400.00001525878906 }, "post_job_actions": { "RenameDatasetActionoutput1": { "action_arguments": { "newname": "Read alignments (SAM format)" }, "action_type": "RenameDatasetAction", "output_name": "output1" } }, "tool_errors": null, "tool_id": "bam_to_sam", "tool_state": "{\"header\": \"\\\"True\\\"\", \"input1\": \"null\", \"chromInfo\": \"\\\"/srv/galaxy/galaxy-dist/tool-data/shared/ucsc/chrom/?.len\\\"\", \"__page__\": 0}", "tool_version": "1.0.3", "type": "tool", "user_outputs": [] }, "4": { "annotation": "Clusters annotated proximal genes into operons. Parameter to be set: op_gap. Genes separated by a gap smaller than op_gap are clustered (suggested value: 10 to 150).", "id": 4, "input_connections": { "formatType|inputFileName": { "id": 2, "output_name": "outputFile" } }, "inputs": [ { "description": "runtime parameter for tool clusterize", "name": "distance" } ], "name": "clusterize", "outputs": [ { "name": "outputFileGff", "type": "gff3" } ], "position": { "left": 700, "top": 200.00001525878906 }, "post_job_actions": { "RenameDatasetActionoutputFileGff": { "action_arguments": { "newname": "Annotation with operon-like features" }, "action_type": "RenameDatasetAction", "output_name": "outputFileGff" } }, "tool_errors": null, "tool_id": "toolshed.g2.bx.psu.edu/repos/yufei-luo/s_mart/MergingDataClusterize/1.0.0", "tool_state": "{\"normalize\": \"\\\"False\\\"\", \"__page__\": 0, \"formatType\": \"{\\\"inputFileName\\\": null, \\\"__current_case__\\\": 1, \\\"FormatInputFileName\\\": \\\"gff\\\"}\", \"distance\": \"{\\\"__class__\\\": \\\"RuntimeValue\\\"}\", \"colinear\": \"\\\"True\\\"\", \"chromInfo\": \"\\\"/srv/galaxy/galaxy-dist/tool-data/shared/ucsc/chrom/?.len\\\"\"}", "tool_version": "1.0.0", "type": "tool", "user_outputs": [] }, "5": { "annotation": "Clusters overlapping or proximal reads to produce read clusters. Parameter to be set: clust_gap. Reads separated by a gap smaller than clust_gap are clustered (suggested value: 0 to 20 ; a value of 0 will cluster only overlapping reads while a value of 20 will cluster reads even if they are 20-nt apart)", "id": 5, "input_connections": { "formatType|inputFileName": { "id": 3, "output_name": "output1" } }, "inputs": [ { "description": "runtime parameter for tool clusterize", "name": "distance" } ], "name": "clusterize", "outputs": [ { "name": "outputFileGff", "type": "gff3" } ], "position": { "left": 662, "top": 505.00001525878906 }, "post_job_actions": { "RenameDatasetActionoutputFileGff": { "action_arguments": { "newname": "Read clusters (with exon)" }, "action_type": "RenameDatasetAction", "output_name": "outputFileGff" } }, "tool_errors": null, "tool_id": "toolshed.g2.bx.psu.edu/repos/yufei-luo/s_mart/MergingDataClusterize/1.0.0", "tool_state": "{\"normalize\": \"\\\"False\\\"\", \"__page__\": 0, \"formatType\": \"{\\\"inputFileName\\\": null, \\\"__current_case__\\\": 4, \\\"FormatInputFileName\\\": \\\"sam\\\"}\", \"distance\": \"{\\\"__class__\\\": \\\"RuntimeValue\\\"}\", \"colinear\": \"\\\"True\\\"\", \"chromInfo\": \"\\\"/srv/galaxy/galaxy-dist/tool-data/shared/ucsc/chrom/?.len\\\"\"}", "tool_version": "1.0.0", "type": "tool", "user_outputs": [] }, "6": { "annotation": "Technical step: suppresses unwanted \"exon\" features created by the previous clustering step.", "id": 6, "input_connections": { "inputFile": { "id": 4, "output_name": "outputFileGff" } }, "inputs": [], "name": "remove exon lines", "outputs": [ { "name": "outputFile", "type": "gff3" } ], "position": { "left": 892, "top": 221.00001525878906 }, "post_job_actions": { "RenameDatasetActionoutputFile": { "action_arguments": { "newname": "Annotation with operon-like (without exon)" }, "action_type": "RenameDatasetAction", "output_name": "outputFile" } }, "tool_errors": null, "tool_id": "toolshed.g2.bx.psu.edu/repos/yufei-luo/s_mart/removeExonLines/1.0.0", "tool_state": "{\"__page__\": 0, \"chromInfo\": \"\\\"/srv/galaxy/galaxy-dist/tool-data/shared/ucsc/chrom/?.len\\\"\", \"inputFile\": \"null\"}", "tool_version": "1.0.0", "type": "tool", "user_outputs": [] }, "7": { "annotation": "Technical step: suppresses unwanted \"exon\" features created by the previous clustering step.", "id": 7, "input_connections": { "inputFile": { "id": 5, "output_name": "outputFileGff" } }, "inputs": [], "name": "remove exon lines", "outputs": [ { "name": "outputFile", "type": "gff3" } ], "position": { "left": 892, "top": 505.00001525878906 }, "post_job_actions": { "RenameDatasetActionoutputFile": { "action_arguments": { "newname": "Read clusters" }, "action_type": "RenameDatasetAction", "output_name": "outputFile" } }, "tool_errors": null, "tool_id": "toolshed.g2.bx.psu.edu/repos/yufei-luo/s_mart/removeExonLines/1.0.0", "tool_state": "{\"__page__\": 0, \"chromInfo\": \"\\\"/srv/galaxy/galaxy-dist/tool-data/shared/ucsc/chrom/?.len\\\"\", \"inputFile\": \"null\"}", "tool_version": "1.0.0", "type": "tool", "user_outputs": [] }, "8": { "annotation": "Technical step: orders the features by increasing positions.", "id": 8, "input_connections": { "formatType|inputFileName": { "id": 6, "output_name": "outputFile" } }, "inputs": [], "name": "collapse reads", "outputs": [ { "name": "outputFileGff", "type": "gff3" } ], "position": { "left": 1072, "top": 261.00001525878906 }, "post_job_actions": { "RenameDatasetActionoutputFileGff": { "action_arguments": { "newname": "Annotation list with operon-like feature" }, "action_type": "RenameDatasetAction", "output_name": "outputFileGff" } }, "tool_errors": null, "tool_id": "toolshed.g2.bx.psu.edu/repos/yufei-luo/s_mart/collapseReads/1.0.0", "tool_state": "{\"__page__\": 0, \"formatType\": \"{\\\"inputFileName\\\": null, \\\"__current_case__\\\": 3, \\\"FormatInputFileName\\\": \\\"gff3\\\"}\", \"chromInfo\": \"\\\"/srv/galaxy/galaxy-dist/tool-data/shared/ucsc/chrom/?.len\\\"\", \"strand\": \"\\\"False\\\"\"}", "tool_version": "1.0.0", "type": "tool", "user_outputs": [] }, "9": { "annotation": "Selects read clusters that form new independent annotations. Parameter to be set: RNA_gap. Read clusters at a distance from existing annotations larger than RNA_gap are selected (suggested value: 25).", "id": 9, "input_connections": { "formatType2|inputFileName2": { "id": 8, "output_name": "outputFileGff" }, "formatType|inputFileName1": { "id": 7, "output_name": "outputFile" } }, "inputs": [ { "description": "runtime parameter for tool compare overlapping small query", "name": "OptionDistance" } ], "name": "compare overlapping small query", "outputs": [ { "name": "outputFileGff", "type": "gff3" } ], "position": { "left": 1197, "top": 521.0000152587891 }, "post_job_actions": { "RenameDatasetActionoutputFileGff": { "action_arguments": { "newname": "Read clusters not overlapping annotation" }, "action_type": "RenameDatasetAction", "output_name": "outputFileGff" } }, "tool_errors": null, "tool_id": "toolshed.g2.bx.psu.edu/repos/yufei-luo/s_mart/CompareOverlappingSmallQuery/1.0.0", "tool_state": "{\"OptionInclusionQuery\": \"\\\"False\\\"\", \"NotOverlapping\": \"\\\"False\\\"\", \"formatType\": \"{\\\"FormatInputFileName1\\\": \\\"gff3\\\", \\\"inputFileName1\\\": null, \\\"__current_case__\\\": 3}\", \"InvertMatch\": \"\\\"True\\\"\", \"__page__\": 0, \"OptionInclusionRef\": \"\\\"False\\\"\", \"OptionDistance\": \"{\\\"distance\\\": {\\\"__class__\\\": \\\"RuntimeValue\\\"}, \\\"Dist\\\": \\\"Yes\\\", \\\"__current_case__\\\": 0}\", \"formatType2\": \"{\\\"FormatInputFileName2\\\": \\\"gff3\\\", \\\"inputFileName2\\\": null, \\\"__current_case__\\\": 3}\", \"OptionPcOverlapQuery\": \"{\\\"present\\\": \\\"No\\\", \\\"__current_case__\\\": 1}\", \"OptionMinOverlap\": \"{\\\"present\\\": \\\"No\\\", \\\"__current_case__\\\": 1}\", \"OptionCollinearOrAntiSens\": \"{\\\"__current_case__\\\": 0, \\\"OptionCA\\\": \\\"Collinear\\\"}\", \"OptionPcOverlapRef\": \"{\\\"present\\\": \\\"No\\\", \\\"__current_case__\\\": 1}\", \"chromInfo\": \"\\\"/srv/galaxy/galaxy-dist/tool-data/shared/ucsc/chrom/?.len\\\"\"}", "tool_version": "1.0.0", "type": "tool", "user_outputs": [] }, "10": { "annotation": "Selects read clusters that overlap annotations in the same direction. Parameter to be set: RNA_gap. A read cluster separated from an annotation by a gap smaller than RNA_gap is selected. Caution: use same RNA_gap value as in previous step!", "id": 10, "input_connections": { "formatType2|inputFileName2": { "id": 8, "output_name": "outputFileGff" }, "formatType|inputFileName1": { "id": 7, "output_name": "outputFile" } }, "inputs": [ { "description": "runtime parameter for tool compare overlapping small query", "name": "OptionDistance" } ], "name": "compare overlapping small query", "outputs": [ { "name": "outputFileGff", "type": "gff3" } ], "position": { "left": 1407, "top": 328.00001525878906 }, "post_job_actions": { "RenameDatasetActionoutputFileGff": { "action_arguments": { "newname": "Read clusters overlapping annotation" }, "action_type": "RenameDatasetAction", "output_name": "outputFileGff" } }, "tool_errors": null, "tool_id": "toolshed.g2.bx.psu.edu/repos/yufei-luo/s_mart/CompareOverlappingSmallQuery/1.0.0", "tool_state": "{\"OptionInclusionQuery\": \"\\\"False\\\"\", \"NotOverlapping\": \"\\\"False\\\"\", \"formatType\": \"{\\\"FormatInputFileName1\\\": \\\"gff3\\\", \\\"inputFileName1\\\": null, \\\"__current_case__\\\": 3}\", \"InvertMatch\": \"\\\"False\\\"\", \"__page__\": 0, \"OptionInclusionRef\": \"\\\"False\\\"\", \"OptionDistance\": \"{\\\"distance\\\": {\\\"__class__\\\": \\\"RuntimeValue\\\"}, \\\"Dist\\\": \\\"Yes\\\", \\\"__current_case__\\\": 0}\", \"formatType2\": \"{\\\"FormatInputFileName2\\\": \\\"gff3\\\", \\\"inputFileName2\\\": null, \\\"__current_case__\\\": 3}\", \"OptionPcOverlapQuery\": \"{\\\"present\\\": \\\"No\\\", \\\"__current_case__\\\": 1}\", \"OptionMinOverlap\": \"{\\\"present\\\": \\\"No\\\", \\\"__current_case__\\\": 1}\", \"OptionCollinearOrAntiSens\": \"{\\\"__current_case__\\\": 0, \\\"OptionCA\\\": \\\"Collinear\\\"}\", \"OptionPcOverlapRef\": \"{\\\"present\\\": \\\"No\\\", \\\"__current_case__\\\": 1}\", \"chromInfo\": \"\\\"/srv/galaxy/galaxy-dist/tool-data/shared/ucsc/chrom/?.len\\\"\"}", "tool_version": "1.0.0", "type": "tool", "user_outputs": [] }, "11": { "annotation": "Selection of read clusters that are antisense of an extended annotation. This step produces a first list of antisense candidates.", "id": 11, "input_connections": { "formatType2|inputFileName2": { "id": 8, "output_name": "outputFileGff" }, "formatType|inputFileName1": { "id": 9, "output_name": "outputFileGff" } }, "inputs": [], "name": "compare overlapping small query", "outputs": [ { "name": "outputFileGff", "type": "gff3" } ], "position": { "left": 1660, "top": 531.0000152587891 }, "post_job_actions": { "RenameDatasetActionoutputFileGff": { "action_arguments": { "newname": "first list of asRNA" }, "action_type": "RenameDatasetAction", "output_name": "outputFileGff" } }, "tool_errors": null, "tool_id": "toolshed.g2.bx.psu.edu/repos/yufei-luo/s_mart/CompareOverlappingSmallQuery/1.0.0", "tool_state": "{\"OptionInclusionQuery\": \"\\\"False\\\"\", \"NotOverlapping\": \"\\\"False\\\"\", \"formatType\": \"{\\\"FormatInputFileName1\\\": \\\"gff3\\\", \\\"inputFileName1\\\": null, \\\"__current_case__\\\": 3}\", \"InvertMatch\": \"\\\"False\\\"\", \"__page__\": 0, \"OptionInclusionRef\": \"\\\"False\\\"\", \"OptionDistance\": \"{\\\"Dist\\\": \\\"No\\\", \\\"__current_case__\\\": 1}\", \"formatType2\": \"{\\\"FormatInputFileName2\\\": \\\"gff3\\\", \\\"inputFileName2\\\": null, \\\"__current_case__\\\": 3}\", \"OptionPcOverlapQuery\": \"{\\\"present\\\": \\\"Yes\\\", \\\"__current_case__\\\": 0, \\\"minOverlap\\\": \\\"100\\\"}\", \"OptionMinOverlap\": \"{\\\"present\\\": \\\"No\\\", \\\"__current_case__\\\": 1}\", \"OptionCollinearOrAntiSens\": \"{\\\"__current_case__\\\": 1, \\\"OptionCA\\\": \\\"AntiSens\\\"}\", \"OptionPcOverlapRef\": \"{\\\"present\\\": \\\"No\\\", \\\"__current_case__\\\": 1}\", \"chromInfo\": \"\\\"/srv/galaxy/galaxy-dist/tool-data/shared/ucsc/chrom/?.len\\\"\"}", "tool_version": "1.0.0", "type": "tool", "user_outputs": [] }, "12": { "annotation": "Selection of read clusters that are outside and not in antisense of an extended annotation. This step produces a first list of small RNAs.", "id": 12, "input_connections": { "formatType2|inputFileName2": { "id": 8, "output_name": "outputFileGff" }, "formatType|inputFileName1": { "id": 9, "output_name": "outputFileGff" } }, "inputs": [], "name": "compare overlapping small query", "outputs": [ { "name": "outputFileGff", "type": "gff3" } ], "position": { "left": 1665, "top": 673 }, "post_job_actions": { "RenameDatasetActionoutputFileGff": { "action_arguments": { "newname": "first list of sRNA" }, "action_type": "RenameDatasetAction", "output_name": "outputFileGff" } }, "tool_errors": null, "tool_id": "toolshed.g2.bx.psu.edu/repos/yufei-luo/s_mart/CompareOverlappingSmallQuery/1.0.0", "tool_state": "{\"OptionInclusionQuery\": \"\\\"False\\\"\", \"NotOverlapping\": \"\\\"False\\\"\", \"formatType\": \"{\\\"FormatInputFileName1\\\": \\\"gff3\\\", \\\"inputFileName1\\\": null, \\\"__current_case__\\\": 3}\", \"InvertMatch\": \"\\\"True\\\"\", \"__page__\": 0, \"OptionInclusionRef\": \"\\\"False\\\"\", \"OptionDistance\": \"{\\\"Dist\\\": \\\"No\\\", \\\"__current_case__\\\": 1}\", \"formatType2\": \"{\\\"FormatInputFileName2\\\": \\\"gff3\\\", \\\"inputFileName2\\\": null, \\\"__current_case__\\\": 3}\", \"OptionPcOverlapQuery\": \"{\\\"present\\\": \\\"Yes\\\", \\\"__current_case__\\\": 0, \\\"minOverlap\\\": \\\"100\\\"}\", \"OptionMinOverlap\": \"{\\\"present\\\": \\\"No\\\", \\\"__current_case__\\\": 1}\", \"OptionCollinearOrAntiSens\": \"{\\\"__current_case__\\\": 1, \\\"OptionCA\\\": \\\"AntiSens\\\"}\", \"OptionPcOverlapRef\": \"{\\\"present\\\": \\\"No\\\", \\\"__current_case__\\\": 1}\", \"chromInfo\": \"\\\"/srv/galaxy/galaxy-dist/tool-data/shared/ucsc/chrom/?.len\\\"\"}", "tool_version": "1.0.0", "type": "tool", "user_outputs": [] }, "13": { "annotation": "Technical step: merging of annotations and their overlapping read clusters. This will be used to extend annotation.", "id": 13, "input_connections": { "input1": { "id": 8, "output_name": "outputFileGff" }, "queries_0|input2": { "id": 10, "output_name": "outputFileGff" } }, "inputs": [], "name": "Concatenate datasets", "outputs": [ { "name": "out_file1", "type": "input" } ], "position": { "left": 1725, "top": 228.00001525878906 }, "post_job_actions": { "RenameDatasetActionout_file1": { "action_arguments": { "newname": "Annotations and read clusters overlapping them" }, "action_type": "RenameDatasetAction", "output_name": "out_file1" } }, "tool_errors": null, "tool_id": "cat1", "tool_state": "{\"__page__\": 0, \"input1\": \"null\", \"chromInfo\": \"\\\"/srv/galaxy/galaxy-dist/tool-data/shared/ucsc/chrom/?.len\\\"\", \"queries\": \"[{\\\"input2\\\": null, \\\"__index__\\\": 0}]\"}", "tool_version": "1.0.0", "type": "tool", "user_outputs": [] }, "14": { "annotation": "Filters antisense RNAs based on their size. Parameter to be set: as_min_size. as_min_size is the minimal size for calling an antisense RNA (suggestion: 50).", "id": 14, "input_connections": { "formatType|inputFileName": { "id": 11, "output_name": "outputFileGff" } }, "inputs": [ { "description": "runtime parameter for tool restrict from size", "name": "OptionMin" } ], "name": "restrict from size", "outputs": [ { "name": "outputFileGff", "type": "gff3" } ], "position": { "left": 1959.2166748046875, "top": 548.4833526611328 }, "post_job_actions": { "RenameDatasetActionoutputFileGff": { "action_arguments": { "newname": "asRNA list modified (filtered on their size)" }, "action_type": "RenameDatasetAction", "output_name": "outputFileGff" } }, "tool_errors": null, "tool_id": "toolshed.g2.bx.psu.edu/repos/yufei-luo/s_mart/restrictFromSize/1.0.0", "tool_state": "{\"OptionMin\": \"{\\\"minimum\\\": \\\"Yes\\\", \\\"__current_case__\\\": 0, \\\"min\\\": {\\\"__class__\\\": \\\"RuntimeValue\\\"}}\", \"__page__\": 0, \"formatType\": \"{\\\"inputFileName\\\": null, \\\"__current_case__\\\": 4, \\\"FormatInputFileName\\\": \\\"gff3\\\"}\", \"OptionMax\": \"{\\\"maximum\\\": \\\"No\\\", \\\"__current_case__\\\": 1}\"}", "tool_version": "1.0.0", "type": "tool", "user_outputs": [] }, "15": { "annotation": "Filters small RNAs based on their size. Parameter to be set: sRNA_min_size. sRNA_min_size is the minimal size for calling a small RNA (suggestion: 50).", "id": 15, "input_connections": { "formatType|inputFileName": { "id": 12, "output_name": "outputFileGff" } }, "inputs": [ { "description": "runtime parameter for tool restrict from size", "name": "OptionMin" } ], "name": "restrict from size", "outputs": [ { "name": "outputFileGff", "type": "gff3" } ], "position": { "left": 1954, "top": 683 }, "post_job_actions": { "RenameDatasetActionoutputFileGff": { "action_arguments": { "newname": "sRNA list modified (filtered on their size)" }, "action_type": "RenameDatasetAction", "output_name": "outputFileGff" } }, "tool_errors": null, "tool_id": "toolshed.g2.bx.psu.edu/repos/yufei-luo/s_mart/restrictFromSize/1.0.0", "tool_state": "{\"OptionMin\": \"{\\\"minimum\\\": \\\"Yes\\\", \\\"__current_case__\\\": 0, \\\"min\\\": {\\\"__class__\\\": \\\"RuntimeValue\\\"}}\", \"__page__\": 0, \"formatType\": \"{\\\"inputFileName\\\": null, \\\"__current_case__\\\": 4, \\\"FormatInputFileName\\\": \\\"gff3\\\"}\", \"chromInfo\": \"\\\"/srv/galaxy/galaxy-dist/tool-data/shared/ucsc/chrom/?.len\\\"\", \"OptionMax\": \"{\\\"maximum\\\": \\\"No\\\", \\\"__current_case__\\\": 1}\"}", "tool_version": "1.0.0", "type": "tool", "user_outputs": [] }, "16": { "annotation": "Extend annotations with overlapping or proximal read clusters in the same direction. Parameter to be set: RNA_gap. Read cluster and annotations separated by a gap smaller than RNA_gap are clustered. Caution: use the same RNA_gap value as previous step!", "id": 16, "input_connections": { "formatType|inputFileName": { "id": 13, "output_name": "out_file1" } }, "inputs": [ { "description": "runtime parameter for tool clusterize", "name": "distance" } ], "name": "clusterize", "outputs": [ { "name": "outputFileGff", "type": "gff3" } ], "position": { "left": 1963, "top": 257.00001525878906 }, "post_job_actions": { "RenameDatasetActionoutputFileGff": { "action_arguments": { "newname": "Extended annotation (with exon)" }, "action_type": "RenameDatasetAction", "output_name": "outputFileGff" } }, "tool_errors": null, "tool_id": "toolshed.g2.bx.psu.edu/repos/yufei-luo/s_mart/MergingDataClusterize/1.0.0", "tool_state": "{\"normalize\": \"\\\"False\\\"\", \"__page__\": 0, \"formatType\": \"{\\\"inputFileName\\\": null, \\\"__current_case__\\\": 3, \\\"FormatInputFileName\\\": \\\"gff3\\\"}\", \"distance\": \"{\\\"__class__\\\": \\\"RuntimeValue\\\"}\", \"colinear\": \"\\\"True\\\"\", \"chromInfo\": \"\\\"/srv/galaxy/galaxy-dist/tool-data/shared/ucsc/chrom/?.len\\\"\"}", "tool_version": "1.0.0", "type": "tool", "user_outputs": [] }, "17": { "annotation": "Filters antisense RNA candidates based on their number of overlapping reads. Parameter to be set: as_min_reads. 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\"\\\"/srv/galaxy/galaxy-dist/tool-data/shared/ucsc/chrom/?.len\\\"\", \"__page__\": 0}", "tool_version": "1.0.0", "type": "tool", "user_outputs": [] }, "37": { "annotation": "Technical step: change feature name from operon to operon_spacer", "id": 37, "input_connections": { "inputFile": { "id": 34, "output_name": "out_file1" } }, "inputs": [], "name": "change GFF features", "outputs": [ { "name": "outputFile", "type": "gff" } ], "position": { "left": 3686, "top": 387.53334045410156 }, "post_job_actions": { "RenameDatasetActionoutputFile": { "action_arguments": { "newname": "operon_spacer list" }, "action_type": "RenameDatasetAction", "output_name": "outputFile" } }, "tool_errors": null, "tool_id": "toolshed.g2.bx.psu.edu/repos/yufei-luo/s_mart/changeGffFeatures/1.0.0", "tool_state": "{\"__page__\": 0, \"inputFeature\": \"\\\"operon\\\"\", \"outputFeature\": \"\\\"operon_spacer\\\"\", \"inputFile\": \"null\"}", "tool_version": "1.0.0", "type": "tool", "user_outputs": [] }, "38": { "annotation": "Technical step: change feature name from urt3 to 3_extension", "id": 38, "input_connections": { "inputFile": { "id": 35, "output_name": "out_file1" } }, "inputs": [], "name": "change GFF features", "outputs": [ { "name": "outputFile", "type": "gff" } ], "position": { "left": 3682, "top": 481.53334045410156 }, "post_job_actions": { "RenameDatasetActionoutputFile": { "action_arguments": { "newname": "3_extension list" }, "action_type": "RenameDatasetAction", "output_name": "outputFile" } }, "tool_errors": null, "tool_id": "toolshed.g2.bx.psu.edu/repos/yufei-luo/s_mart/changeGffFeatures/1.0.0", "tool_state": "{\"__page__\": 0, \"inputFeature\": \"\\\"utr3\\\"\", \"outputFeature\": \"\\\"3_extension\\\"\", \"inputFile\": \"null\"}", "tool_version": "1.0.0", "type": "tool", "user_outputs": [] }, "39": { "annotation": "Technical step: change feature name from urt5 to 5_extension", "id": 39, "input_connections": { "inputFile": { "id": 36, "output_name": "out_file1" } }, 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Parameter to be set: 5_extension_min_size. 5' extensions larger than 5_extension_min_size are selected (suggestion value: 50).", "id": 40, "input_connections": { "formatType|inputFileName": { "id": 39, "output_name": "outputFile" } }, "inputs": [ { "description": "runtime parameter for tool restrict from size", "name": "OptionMin" } ], "name": "restrict from size", "outputs": [ { "name": "outputFileGff", "type": "gff3" } ], "position": { "left": 3823, "top": 580.0000152587891 }, "post_job_actions": { "RenameDatasetActionoutputFileGff": { "action_arguments": { "newname": "long 5_extensions list modified (filtered on their size)" }, "action_type": "RenameDatasetAction", "output_name": "outputFileGff" } }, "tool_errors": null, "tool_id": "toolshed.g2.bx.psu.edu/repos/yufei-luo/s_mart/restrictFromSize/1.0.0", "tool_state": "{\"OptionMin\": \"{\\\"minimum\\\": \\\"Yes\\\", \\\"__current_case__\\\": 0, \\\"min\\\": {\\\"__class__\\\": \\\"RuntimeValue\\\"}}\", \"__page__\": 0, \"formatType\": \"{\\\"inputFileName\\\": null, \\\"__current_case__\\\": 4, \\\"FormatInputFileName\\\": \\\"gff3\\\"}\", \"chromInfo\": \"\\\"/srv/galaxy/galaxy-dist/tool-data/shared/ucsc/chrom/?.len\\\"\", \"OptionMax\": \"{\\\"maximum\\\": \\\"No\\\", \\\"__current_case__\\\": 1}\"}", "tool_version": "1.0.0", "type": "tool", "user_outputs": [] }, "41": { "annotation": "Filters long 5 prime extension candidates based on their number of overlapping reads. Parameter to be set: 5_extension_min_reads. Long 5 prime extensions with a number of reads higher than 5_extension_min_reads are selected (suggested value: 10).", "id": 41, "input_connections": { "formatType|inputFileName": { "id": 40, "output_name": "outputFileGff" } }, "inputs": [ { "description": "runtime parameter for tool select by tag", "name": "OptionMin" } ], "name": "select by tag", "outputs": [ { "name": "outputFileGff", "type": "gff3" } ], "position": { "left": 3997, "top": 586.0000152587891 }, "post_job_actions": { "RenameDatasetActionoutputFileGff": { "action_arguments": { "newname": "long 5_extensions list modified (filtered on their number of overlapping reads)" }, "action_type": "RenameDatasetAction", "output_name": "outputFileGff" } }, "tool_errors": null, "tool_id": "toolshed.g2.bx.psu.edu/repos/yufei-luo/s_mart/SelectByTag/1.0.0", "tool_state": "{\"__page__\": 0, \"formatType\": \"{\\\"inputFileName\\\": null, \\\"__current_case__\\\": 2, \\\"FormatInputFileName\\\": \\\"gff3\\\"}\", \"OptionMin\": \"{\\\"minimum\\\": \\\"Yes\\\", \\\"__current_case__\\\": 0, \\\"min\\\": {\\\"__class__\\\": \\\"RuntimeValue\\\"}}\", \"Tag\": \"\\\"nbOverlappingReads\\\"\", \"OptionMax\": \"{\\\"maximum\\\": \\\"No\\\", \\\"__current_case__\\\": 1}\", \"OptionValue\": \"{\\\"Value\\\": \\\"No\\\", \\\"__current_case__\\\": 1}\", \"chromInfo\": \"\\\"/srv/galaxy/galaxy-dist/tool-data/shared/ucsc/chrom/?.len\\\"\", \"OptionDefault\": \"{\\\"default\\\": \\\"Yes\\\", \\\"defaultValue\\\": \\\"0.0\\\", \\\"__current_case__\\\": 0}\"}", "tool_version": "1.0.0", "type": "tool", "user_outputs": [] }, "42": { "annotation": "Uses an RGB color tag for visualization of long 5_extension in the Artemis environment.", "id": 42, "input_connections": { "referenciesFile": { "id": 41, "output_name": "outputFileGff" } }, "inputs": [], "name": "colorGff", "outputs": [ { "name": "outputFile", "type": "gff" } ], "position": { "left": 4176, "top": 591.0000152587891 }, "post_job_actions": { "RenameDatasetActionoutputFile": { "action_arguments": { "newname": "long 5_extensions list" }, "action_type": "RenameDatasetAction", "output_name": "outputFile" } }, "tool_errors": null, "tool_id": "toolshed.g2.bx.psu.edu/repos/clairetn/detrprok_scripts/colorGff/1.0.0", "tool_state": "{\"__page__\": 0, \"chromInfo\": \"\\\"/srv/galaxy/galaxy-dist/tool-data/shared/ucsc/chrom/?.len\\\"\", \"referenciesFile\": \"null\", \"RGBcolor\": \"\\\"255 105 180\\\"\"}", "tool_version": "1.0.0", "type": "tool", "user_outputs": [] } } }