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1 #!/usr/bin/perl
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2
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3 use strict;
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4 use Switch;
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5 use Getopt::Long;
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6 use Bio::SeqIO;
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7
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8 my $PLINK_EXE= "/apps/www/sniplay.cirad.fr/tools/plink/plink-1.07-x86_64/plink";
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9
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10 my $usage = qq~Usage:$0 <args> [<opts>]
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11 where <args> are:
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12 -i, --in <input>
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13 -o, --out <output>
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14 ~;
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15 $usage .= "\n";
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16
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17 my ($in,$out);
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18
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19
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20 GetOptions(
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21 "in=s" => \$in,
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22 "out=s" => \$out
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23 );
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24
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25 die $usage
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26 if ( !$in || !$out);
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27
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28
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29 my $plink_command = $PLINK_EXE . " --file $in --noweb --cluster --matrix --mds-plot 2 --out $out >>$in.plink.log 2>&1";
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30 system($plink_command);
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31
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32 my $awk_cmd = "awk \{\'print \$1\'\} $in.ped";
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33 my $inds = `$awk_cmd`;
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34 my @individuals = split("\n",$inds);
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35
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36
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37 open(my $OUT,">$out.mds_plot.txt");
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38 my $go = 0;
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39 open(my $O,"$out.mds");
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40 while(<$O>)
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41 {
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42 if ($go)
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43 {
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44 my $line = $_;
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45 $line =~s/\n//g;
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46 $line =~s/\r//g;
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47 my @i = split(/\s+/,$line);
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48 my $ind = $i[1];
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49 print $OUT "$ind ".$i[4]." ".$i[5]."\n";
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50 }
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51 if (/C1/){$go = 1;}
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52 }
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53 close($O);
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54 close($OUT);
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55
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56
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57 my $j = 0;
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58 open(my $IBS,">$out.ibs_matrix.txt");
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59 print $IBS "Individuals " . join("\t",@individuals)."\n";
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60 open(my $O2,"$out.mibs");
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61 while(<$O2>)
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62 {
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63 my $line = $_;
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64 $line =~s/\n//g;
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65 $line =~s/\r//g;
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66 my @i = split(/\s+/,$line);
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67 print $IBS $individuals[$j]. " ". join("\t",@i)."\n";
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68 $j++;
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69 }
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70 close($O2);
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71 close($IBS);
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72
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73
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74
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75
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76
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77
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