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1 <tool id="EMBOSS: primersearch81" name="primersearch" version="5.0.0">
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2 <description>Searches DNA sequences for matches with primer pairs</description>
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3 <requirements><requirement type="package" version="5.0.0">emboss</requirement></requirements>
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4 <command>primersearch -seqall $input1 -infile $input2 -outfile $out_file1 -mismatchpercent $mismatchpercent -auto</command>
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5 <inputs>
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6 <param format="fasta" name="input1" type="data">
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7 <label>Main sequences</label>
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8 </param>
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9 <param format="data" name="input2" type="data">
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10 <label>Primer file</label>
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11 </param>
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12 <param name="mismatchpercent" size="4" type="text" value="0">
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13 <label>Allowed percent mismatch</label>
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14 </param>
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15 </inputs>
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16 <outputs>
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17 <data format="primersearch" name="out_file1" />
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18 </outputs>
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19 <tests>
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20 <test>
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21 <param name="input1" value="2.fasta"/>
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22 <param name="input2" value="emboss_primersearch.fasta"/>
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23 <param name="mismatchpercent" value="0"/>
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24 <output name="out_file1" file="emboss_primersearch_out.primersearch"/>
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25 </test>
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26 </tests>
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27 <help>
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28
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29 You can view the original documentation here_.
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30
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31 .. _here: http://emboss.sourceforge.net/apps/release/5.0/emboss/apps/primersearch.html
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32
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33 ------
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34
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35 **Citation**
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36
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37 For the underlying tool, please cite `Rice P, Longden I, Bleasby A. EMBOSS: the European Molecular Biology Open Software Suite. Trends Genet. 2000 Jun;16(6):276-7. <http://www.ncbi.nlm.nih.gov/pubmed/10827456>`_
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38
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39 If you use this tool in Galaxy, please cite `Blankenberg D, Taylor J, Schenck I, He J, Zhang Y, Ghent M, Veeraraghavan N, Albert I, Miller W, Makova KD, Hardison RC, Nekrutenko A. A framework for collaborative analysis of ENCODE data: making large-scale analyses biologist-friendly. Genome Res. 2007 Jun;17(6):960-4. <http://www.ncbi.nlm.nih.gov/pubmed/17568012>`_
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40 </help>
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41 </tool>
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