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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/galaxy_sequence_utils/fastq_trimmer commit bb5df9e62585e12f08dfc0a9f86eec8e205b4845
author | iuc |
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date | Fri, 04 Oct 2024 10:35:28 +0000 |
parents | b63b2bfb3ea3 |
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<tool id="fastq_trimmer" name="FASTQ Trimmer" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" profile="@PROFILE@"> <description>by column</description> <macros> <import>macros.xml</import> </macros> <edam_topics> <edam_topic>topic_0622</edam_topic> </edam_topics> <edam_operations> <edam_operation>operation_3192</edam_operation> </edam_operations> <expand macro="requirements"/> <command><![CDATA[ gx-fastq-trimmer '$input_file' '$output_file' ${offset_type.left_column_offset} ${offset_type.right_column_offset} ${offset_type.base_offset_type} '${input_file.extension[len('fastq'):]}' $keep_zero_length ]]></command> <inputs> <param name="input_file" type="data" format="fastqsanger,fastqcssanger,fastqsanger.gz,fastqcssanger.gz,fastqsanger.bz2,fastqcssanger.bz2" label="FASTQ file"/> <conditional name="offset_type"> <param name="base_offset_type" type="select" label="Define Base Offsets as" help="Use Absolute for fixed length reads (Illumina, SOLiD)<br>Use Percentage for variable length reads (Roche/454)"> <option value="offsets_absolute" selected="true">Absolute Values</option> <option value="offsets_percent">Percentage of Read Length</option> </param> <when value="offsets_absolute"> <param name="left_column_offset" type="integer" min="0" value="0" label="Offset from 5' end" help="Values start at 0, increasing from the left" /> <param name="right_column_offset" type="integer" value="0" label="Offset from 3' end" help="Values start at 0, increasing from the right; use a negative value to remove everything to the right of the absolute value of the position" /> </when> <when value="offsets_percent"> <param name="left_column_offset" type="float" min="0" max="100" value="0" label="Offset from 5' end" /> <param name="right_column_offset" type="float" min="0" max="100" value="0" label="Offset from 3' end" /> </when> </conditional> <param name="keep_zero_length" type="boolean" truevalue="keep_zero_length" falsevalue="exclude_zero_length" checked="false" label="Keep reads with zero length" /> </inputs> <outputs> <data name="output_file" format_source="input_file" /> </outputs> <tests> <test> <!-- Do nothing trim --> <param name="input_file" value="sanger_full_range_original_sanger.fastqsanger" ftype="fastqsanger" /> <param name="base_offset_type" value="offsets_absolute"/> <param name="left_column_offset" value="0"/> <param name="right_column_offset" value="0"/> <param name="keep_zero_length" value="keep_zero_length" /> <output name="output_file" file="sanger_full_range_original_sanger.fastqsanger" ftype="fastqsanger" /> </test> <!-- Trim to empty File --> <test> <param name="input_file" value="sanger_full_range_original_sanger.fastqsanger" ftype="fastqsanger" /> <param name="base_offset_type" value="offsets_absolute"/> <param name="left_column_offset" value="30"/> <param name="right_column_offset" value="64"/> <param name="keep_zero_length" value="exclude_zero_length" /> <output name="output_file" file="empty_file.dat" ftype="fastqsanger" /> </test> <test> <param name="input_file" value="sanger_full_range_original_sanger.fastqsanger" ftype="fastqsanger" /> <param name="base_offset_type" value="offsets_percent"/> <param name="left_column_offset" value="50"/> <param name="right_column_offset" value="50"/> <param name="keep_zero_length" value="exclude_zero_length" /> <output name="output_file" file="empty_file.dat" ftype="fastqsanger" /> </test> <!-- Trim to 4 inner-most bases --> <test> <param name="input_file" value="sanger_full_range_original_sanger.fastqsanger" ftype="fastqsanger" /> <param name="base_offset_type" value="offsets_absolute"/> <param name="left_column_offset" value="45"/> <param name="right_column_offset" value="45"/> <param name="keep_zero_length" value="exclude_zero_length" /> <output name="output_file" file="fastq_trimmer_out1.fastqsanger" ftype="fastqsanger" /> </test> <test> <param name="input_file" value="sanger_full_range_original_sanger.fastqsanger" ftype="fastqsanger" /> <param name="base_offset_type" value="offsets_percent"/> <param name="left_column_offset" value="47.87"/> <param name="right_column_offset" value="47.87"/> <param name="keep_zero_length" value="exclude_zero_length" /> <output name="output_file" file="fastq_trimmer_out1.fastqsanger" ftype="fastqsanger" /> </test> </tests> <help><![CDATA[ **What is does** This tool allows you to trim the ends of reads. You can specify either absolute or percent-based offsets. Offsets are calculated, starting at 0, from the respective end to be trimmed. When using the percent-based method, offsets are rounded to the nearest integer. For example, if you have a read of length 36:: @Some FASTQ Sanger Read CAATATGTNCTCACTGATAAGTGGATATNAGCNCCA + =@@.@;B-%?8>CBA@>7@7BBCA4-48%<;;%<B@ And you set absolute offsets of 2 and 9:: @Some FASTQ Sanger Read ATATGTNCTCACTGATAAGTGGATA + @.@;B-%?8>CBA@>7@7BBCA4-4 Or you set percent offsets of 6% and 20% (corresponds to absolute offsets of 2,7 for a read length of 36):: @Some FASTQ Sanger Read ATATGTNCTCACTGATAAGTGGATATN + @.@;B-%?8>CBA@>7@7BBCA4-48% ----- .. class:: warningmark Trimming a color space read will cause any adapter base to be lost. ]]></help> <citations> <citation type="doi">10.1093/bioinformatics/btq281</citation> </citations> </tool>