Mercurial > repos > devteam > ncbi_blast_plus
annotate tools/ncbi_blast_plus/ncbi_tblastn_wrapper.xml @ 11:4c4a0da938ff draft
Uploaded v0.0.22, now wraps BLAST+ 2.2.28 allowing extended tabular output to include the hit descriptions as column 25.
Supports $GALAXY_SLOTS.
Includes more tests and heavy use of macros.
author | peterjc |
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date | Thu, 05 Dec 2013 06:55:59 -0500 |
parents | 70e7dcbf6573 |
children | 623f727cdff1 |
rev | line source |
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11
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Uploaded v0.0.22, now wraps BLAST+ 2.2.28 allowing extended tabular output to include the hit descriptions as column 25.
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1 <tool id="ncbi_tblastn_wrapper" name="NCBI BLAST+ tblastn" version="0.0.22"> |
3
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2 <description>Search translated nucleotide database with protein query sequence(s)</description> |
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3 <!-- If job splitting is enabled, break up the query file into parts --> |
11
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4 <parallelism method="multi" split_inputs="query" split_mode="to_size" split_size="1000" merge_outputs="output1"></parallelism> |
4c4a0da938ff
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5 <macros> |
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6 <token name="@BINARY@">tblastn</token> |
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7 <import>ncbi_macros.xml</import> |
4c4a0da938ff
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8 </macros> |
4c4a0da938ff
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9 <expand macro="requirements" /> |
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10 <command> |
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11 ## The command is a Cheetah template which allows some Python based syntax. |
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12 ## Lines starting hash hash are comments. Galaxy will turn newlines into spaces |
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13 tblastn |
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14 -query "$query" |
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15 @BLAST_DB_SUBJECT@ |
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16 -evalue $evalue_cutoff |
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17 @BLAST_OUTPUT@ |
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18 @THREADS@ |
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19 #if $adv_opts.adv_opts_selector=="advanced": |
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20 -db_gencode $adv_opts.db_gencode |
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21 -matrix $adv_opts.matrix |
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22 @ADVANCED_OPTIONS@ |
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23 ##Ungapped disabled for now - see comments below |
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24 ##$adv_opts.ungapped |
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25 ## End of advanced options: |
643338ac83c0
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26 #end if |
643338ac83c0
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27 </command> |
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28 |
4c4a0da938ff
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29 <expand macro="stdio" /> |
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30 |
3
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31 <inputs> |
643338ac83c0
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32 <param name="query" type="data" format="fasta" label="Protein query sequence(s)"/> |
11
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33 |
4c4a0da938ff
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34 <expand macro="input_conditional_nucleotide_db" /> |
4c4a0da938ff
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35 <expand macro="input_out_format" /> |
4c4a0da938ff
Uploaded v0.0.22, now wraps BLAST+ 2.2.28 allowing extended tabular output to include the hit descriptions as column 25.
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36 <expand macro="input_evalue" /> |
4c4a0da938ff
Uploaded v0.0.22, now wraps BLAST+ 2.2.28 allowing extended tabular output to include the hit descriptions as column 25.
peterjc
parents:
10
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37 <expand macro="advanced_options"> |
4c4a0da938ff
Uploaded v0.0.22, now wraps BLAST+ 2.2.28 allowing extended tabular output to include the hit descriptions as column 25.
peterjc
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10
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38 <expand macro="input_db_gencode" /> |
4c4a0da938ff
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39 |
4c4a0da938ff
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40 <!-- Could use a select (yes, no, other) where other allows setting 'window locut hicut' --> |
4c4a0da938ff
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41 <expand macro="input_filter_query_default_true" /> |
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42 <expand macro="input_scoring_matrix" /> |
4c4a0da938ff
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43 <expand macro="input_max_hits" /> |
4c4a0da938ff
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44 <expand macro="input_word_size" /> |
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45 <!-- |
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46 Can't use '-ungapped' on its own, error back is: |
4c4a0da938ff
Uploaded v0.0.22, now wraps BLAST+ 2.2.28 allowing extended tabular output to include the hit descriptions as column 25.
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47 Composition-adjusted searched are not supported with an ungapped search, please add -comp_based_stats F or do a gapped search |
4c4a0da938ff
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48 Tried using '-ungapped -comp_based_stats F' and tblastn crashed with 'Attempt to access NULL pointer.' |
4c4a0da938ff
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49 <param name="ungapped" type="boolean" label="Perform ungapped alignment only?" truevalue="-ungapped -comp_based_stats F" falsevalue="" checked="false" /> |
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50 --> |
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51 <expand macro="input_parse_deflines" /> |
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52 </expand> |
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53 </inputs> |
643338ac83c0
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54 <outputs> |
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55 <data name="output1" format="tabular" label="tblastn $query.name vs @ON_DB_SUBJECT@"> |
4c4a0da938ff
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56 <expand macro="output_change_format" /> |
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57 </data> |
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58 </outputs> |
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59 <tests> |
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60 <test> |
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61 <param name="query" value="four_human_proteins.fasta" ftype="fasta" /> |
643338ac83c0
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62 <param name="db_opts_selector" value="file" /> |
643338ac83c0
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63 <param name="subject" value="rhodopsin_nucs.fasta" ftype="fasta" /> |
643338ac83c0
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64 <param name="database" value="" /> |
643338ac83c0
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65 <param name="evalue_cutoff" value="1e-10" /> |
643338ac83c0
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66 <param name="out_format" value="5" /> |
643338ac83c0
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67 <param name="adv_opts_selector" value="advanced" /> |
643338ac83c0
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68 <param name="filter_query" value="false" /> |
643338ac83c0
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69 <param name="matrix" value="BLOSUM80" /> |
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70 <param name="max_hits" value="0" /> |
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71 <param name="word_size" value="0" /> |
643338ac83c0
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72 <param name="parse_deflines" value="false" /> |
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73 <output name="output1" file="tblastn_four_human_vs_rhodopsin.xml" ftype="blastxml" /> |
643338ac83c0
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74 </test> |
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75 <test> |
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76 <param name="query" value="four_human_proteins.fasta" ftype="fasta" /> |
643338ac83c0
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77 <param name="db_opts_selector" value="file" /> |
643338ac83c0
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78 <param name="subject" value="rhodopsin_nucs.fasta" ftype="fasta" /> |
643338ac83c0
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peterjc
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79 <param name="database" value="" /> |
643338ac83c0
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peterjc
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80 <param name="evalue_cutoff" value="1e-10" /> |
643338ac83c0
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81 <param name="out_format" value="ext" /> |
643338ac83c0
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82 <param name="adv_opts_selector" value="advanced" /> |
643338ac83c0
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peterjc
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83 <param name="filter_query" value="false" /> |
643338ac83c0
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peterjc
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84 <param name="matrix" value="BLOSUM80" /> |
643338ac83c0
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85 <param name="max_hits" value="0" /> |
643338ac83c0
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peterjc
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86 <param name="word_size" value="0" /> |
643338ac83c0
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peterjc
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87 <param name="parse_deflines" value="false" /> |
643338ac83c0
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88 <output name="output1" file="tblastn_four_human_vs_rhodopsin_ext.tabular" ftype="tabular" /> |
643338ac83c0
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89 </test> |
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90 <test> |
643338ac83c0
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91 <param name="query" value="four_human_proteins.fasta" ftype="fasta" /> |
643338ac83c0
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peterjc
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92 <param name="db_opts_selector" value="file" /> |
643338ac83c0
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peterjc
parents:
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93 <param name="subject" value="rhodopsin_nucs.fasta" ftype="fasta" /> |
643338ac83c0
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peterjc
parents:
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94 <param name="database" value="" /> |
643338ac83c0
Uploaded v0.0.12b, same code but moving folders around to match all my other tools and make future development simpler.
peterjc
parents:
diff
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|
95 <param name="evalue_cutoff" value="1e-10" /> |
643338ac83c0
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peterjc
parents:
diff
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96 <param name="out_format" value="6" /> |
643338ac83c0
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peterjc
parents:
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97 <param name="adv_opts_selector" value="advanced" /> |
643338ac83c0
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peterjc
parents:
diff
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98 <param name="filter_query" value="false" /> |
643338ac83c0
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peterjc
parents:
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99 <param name="matrix" value="BLOSUM80" /> |
643338ac83c0
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peterjc
parents:
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100 <param name="max_hits" value="0" /> |
643338ac83c0
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peterjc
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101 <param name="word_size" value="0" /> |
643338ac83c0
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peterjc
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102 <param name="parse_deflines" value="false" /> |
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103 <output name="output1" file="tblastn_four_human_vs_rhodopsin.tabular" ftype="tabular" /> |
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104 </test> |
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105 <test> |
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106 <!-- Same as above, but parse deflines - on BLAST 2.2.25+ - 2.2.27+ makes no difference --> |
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107 <param name="query" value="four_human_proteins.fasta" ftype="fasta" /> |
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108 <param name="db_opts_selector" value="file" /> |
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109 <param name="subject" value="rhodopsin_nucs.fasta" ftype="fasta" /> |
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110 <param name="database" value="" /> |
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111 <param name="evalue_cutoff" value="1e-10" /> |
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112 <param name="out_format" value="6" /> |
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113 <param name="adv_opts_selector" value="advanced" /> |
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114 <param name="filter_query" value="false" /> |
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115 <param name="matrix" value="BLOSUM80" /> |
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116 <param name="max_hits" value="0" /> |
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117 <param name="word_size" value="0" /> |
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118 <param name="parse_deflines" value="true" /> |
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119 <output name="output1" file="tblastn_four_human_vs_rhodopsin.tabular" ftype="tabular" /> |
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120 </test> |
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121 <test> |
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122 <param name="query" value="four_human_proteins.fasta" ftype="fasta" /> |
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123 <param name="db_opts_selector" value="file" /> |
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124 <param name="subject" value="rhodopsin_nucs.fasta" ftype="fasta" /> |
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125 <param name="database" value="" /> |
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126 <param name="evalue_cutoff" value="1e-10" /> |
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127 <param name="out_format" value="0 -html" /> |
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128 <param name="adv_opts_selector" value="advanced" /> |
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129 <param name="filter_query" value="false" /> |
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130 <param name="matrix" value="BLOSUM80" /> |
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131 <param name="max_hits" value="0" /> |
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132 <param name="word_size" value="0" /> |
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133 <param name="parse_deflines" value="false" /> |
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134 <output name="output1" file="tblastn_four_human_vs_rhodopsin.html" ftype="html" /> |
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135 </test> |
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136 </tests> |
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137 <help> |
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138 |
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139 @SEARCH_TIME_WARNING@ |
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140 |
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141 **What it does** |
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142 |
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143 Search a *translated nucleotide database* using a *protein query*, |
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144 using the NCBI BLAST+ tblastn command line tool. |
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145 |
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146 @FASTA_WARNING@ |
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147 |
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148 ----- |
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149 |
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150 @OUTPUT_FORMAT@ |
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151 |
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152 ------- |
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153 |
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154 **References** |
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155 |
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156 If you use this Galaxy tool in work leading to a scientific publication please |
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157 cite the following papers: |
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158 |
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159 @REFERENCES@ |
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160 </help> |
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161 </tool> |