view monocle3-plotCells.xml @ 4:016c39d243fb draft

"planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/ commit c388aa3bb719eff471508b6f01cae8583ee7bb1d"

<?xml version="1.0" encoding="utf-8"?>
<tool id="monocle3_plotCells" name="Monocle3 plotCells" version="0.1.5+galaxy0" profile="@PROFILE@">
  <description> in the reduced dimensionality space</description>
  <macros>
    <import>monocle3-macros.xml</import>
  </macros>
  <requirements>
    <requirement type="package" version="0.0.8">monocle3-cli</requirement>
  </requirements>
  <command detect_errors="exit_code"><![CDATA[
LANG=en_US.UTF-8 monocle3 plotCells
    --reduction-method '$(reduction_method)'
    --norm-method '$(norm_method)'
#if $xdim
    --xdim '$(xdim)'
#end if
#if $ydim
    --ydim '$(ydim)'
#end if
#if $color_cells_by
    --color-cells-by '$(color_cells_by)'
#end if
#if $genes
    --genes '$(genes)'
#end if
#if $cell_size
    --cell-size '$(cell_size)'
#end if
#if $alpha
    --alpha '$(alpha)'
#end if
#if $label_cell_groups
    --label-cell-groups
#end if
#if $no_trajectory_graph
    --no-trajectory-graph
#end if
#if $label_groups_by_cluster
    --label-groups-by-cluster
#end if
#if $label_leaves
    --label-leaves
#end if
#if $label_roots
    --label-roots
#end if
#if $label_branch_points
    --label-branch-points
#end if

    @INPUT_OPTS@
    output.png

    @VERBOSE@
]]></command>

  <inputs>
    <expand macro="input_object_params"/>
    <param name="xdim" argument="--xdim" optional="true" type="integer" value="1" label="The column of reducedDimS(cds) to plot on the horizontal axis."/>
    <param name="ydim" argument="--ydim" optional="true" type="integer" value="2" label="The column of reducedDimS(cds) to plot on the vertical axis."/>
    <param name="reduction_method" argument="--reduction-method" type="select" label="The dimensionality reduction for plotting.">
      <option value="UMAP" selected="true">UMAP</option>
      <option value="tSNE">tSNE</option>
      <option value="PCA">PCA</option>
      <option value="LSI">LSI</option>
    </param>
    <param name="color_cells_by" argument="--color-cells-by" optional="true" type="text" value="pseudotime" label="The cell attribute (e.g. the column of pData(cds)) to map to each cell's color, or one of {cluster, partition, pseudotime}."/>
    <param name="genes" argument="--genes" optional="true" type="text" label="A list of gene IDs/short names to plot, one per panel."/>
    <param name="norm_method" argument="--norm-method" type="select" label="Determines how to transform expression values for plotting.">
      <option value="log" selected="true">Size factor correction and log transformation</option>
      <option value="size_only">Size factor correction only</option>
    </param>
    <param name="cell_size" argument="--cell-size" optional="true" type="float" value="1.5" label="The size of the point for each cell."/>
    <param name="alpha" argument="--alpha" optional="true" type="float" value="1" label="The alpha aesthetics for the original cell points, useful to highlight the learned principal graph."/>
    <param name="label_cell_groups" argument="--label-cell-groups" type="boolean" checked="true" label="If set, display the cell group names directly on the plot. Otherwise include a color legend on the side of the plot."/>
    <param name="no_trajectory_graph" argument="--no-trajectory-graph" type="boolean" checked="false" label="When this option is set, skip displaying the trajectory graph inferred by learn_graph()."/>
    <param name="label_groups_by_cluster" argument="--label-groups-by-cluster" type="boolean" checked="false" label="If set, and setting --color-cells-by to something other than cluster, label the cells of each cluster independently. Can result in duplicate labels being present in the manifold."/>
	<param name="label_leaves" argument="--label-leaves" type="boolean" checked="false" label="If set, label the leaves of the principal graph."/>
	<param name="label_roots" argument="--label-roots" type="boolean" checked="false" label="If set, label the roots of the principal graph."/>
	<param name="label_branch_points" argument="--label-branch-points" type="boolean" checked="false" label="If set, label the branch points of the principal graph."/>
    <expand macro="verbose_flag"/>
  </inputs>

  <outputs>
    <data name="output_tsv" format="png" from_work_dir="output.png" label="${tool.name} on ${on_string}: png"/>
  </outputs>

  <tests>
    <test>
      <param name="input_object_file" value="input.RDS"/>
      <param name="input_object_format" value="cds3"/>
      <param name="reduction_method" value="UMAP"/>
      <param name="xdim" value="1"/>
      <param name="ydim" value="2"/>
      <param name="color_cells_by" value="pseudotime"/>
      <param name="norm_method" value="log"/>
      <param name="cell_size" value="1.5"/>
      <param name="alpha" value="1"/>
      <output name="output_tsv" file="output.tsv" ftype="tsv" compare="sim_size"/>
    </test>
  </tests>

  <help><![CDATA[
=====================================
Monocle3 cell plotting (`plot_cells`)
=====================================

Display various information about cells (clustering, pseudotime, the inferred graph,
user-provided annotations, gene expression) in reduced dimensions.

@HELP@

@VERSION_HISTORY@
]]></help>
  <expand macro="citations"/>
</tool>