Mercurial > repos > ebi-gxa > sc3_calc_transfs
diff sc3-calc-transfs.xml @ 0:b06019da7b5a draft default tip
planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/ commit 9bf9a6e46a330890be932f60d1d996dd166426c4
author | ebi-gxa |
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date | Wed, 03 Apr 2019 11:34:00 -0400 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/sc3-calc-transfs.xml Wed Apr 03 11:34:00 2019 -0400 @@ -0,0 +1,46 @@ +<?xml version="1.0" encoding="utf-8"?> +<tool id="sc3_calc_transfs" name="SC3 Calculate Transformations" version="@TOOL_VERSION@+galaxy0"> + <description>of distances using PCA and graph Laplacian</description> + <macros> + <import>sc3-macros.xml</import> + </macros> + <expand macro="requirements" /> + <command detect_errors="exit_code"><![CDATA[ +sc3-sc3-calc-transfs.R -i '${sc3_distances}' -o '${sc3_transformed}' + ]]></command> + + <inputs> + <param name="sc3_distances" type="data" format="rdata" label="Serialised sc3 SingleCellExperiment object with distances calculated"/> + </inputs> + + <outputs> + <data name="sc3_transformed" format="rdata" label="${tool.name} on ${on_string}: serialised sc3 SingleCellExperiment object with transformed distances"/> + </outputs> + + <tests> + <test> + <param name="sc3_distances" value="sc3_distances.rdata"/> + <output name="sc3_transformed" file="sc3_transformed.rdata"/> + </test> + </tests> + + <help><![CDATA[ +============================================== +This function calculate transformed distances. +============================================== + +All items of the sc3 slot of the metadata(object) are transformed using either +principal component analysis (PCA) or by calculating the eigenvectors of the +associated graph Laplacian. The columns of the resulting matrices are then +sorted in descending order by their corresponding eigenvalues. The first d +columns (where d = max(metadata(object)$sc3$n_dim)) of each transformation are +then written to the transformations item of the sc3 slot. Additionally, this +function also removes the previously calculated distances from the sc3 slot, as +they are not needed for further analysis. + +@HELP@ + +@VERSION_HISTORY@ + ]]></help> + <expand macro="citations" /> +</tool>