changeset 28:64dd8bb151e1 draft default tip

planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit 1161fdc6d874689d9a7a10bbe22b7758b2edc4bf-dirty

--- a/scanpy-find-variable-genes.xml	Tue Feb 27 16:42:27 2024 +0000
+++ b/scanpy-find-variable-genes.xml	Wed May 15 09:39:42 2024 +0000
@@ -1,5 +1,5 @@
 <?xml version="1.0" encoding="utf-8"?>
-<tool id="scanpy_find_variable_genes" name="Scanpy FindVariableGenes" version="@TOOL_VERSION@+galaxy0" profile="@PROFILE@">
+<tool id="scanpy_find_variable_genes" name="Scanpy FindVariableGenes" version="@TOOL_VERSION@+galaxy1" profile="@PROFILE@">
   <description>based on normalised dispersion of expression</description>
   <macros>
     <import>scanpy_macros2.xml</import>
@@ -26,6 +26,12 @@
 #if $batch_key
     --batch-key ${batch_key}
 #end if
+#if $never_hvg
+    --never-hv-genes-file '${never_hvg}'
+#end if
+#if $always_hvg
+    --always-hv-genes-file '${always_hvg}'
+#end if
     @INPUT_OPTS@
     @OUTPUT_OPTS@
 ]]></command>
@@ -54,6 +60,8 @@
     <param name="filter" argument="--subset" type="boolean" truevalue="--subset" falsevalue="" checked="false"
            label="Remove genes not marked as highly variable" help="When set, inplace subset to highly-variable genes, otherwise only flag highly-variable genes."/>
     <param name="batch_key" argument="--batch-key" type="text" label="Batch key" optional="true" help="If specified, highly-variable genes are selected within each batch separately and merged. This simple process avoids the selection of batch-specific genes and acts as a lightweight batch correction method. For all flavors, genes are first sorted by how many batches they are a HVG. For dispersion-based flavors ties are broken by normalized dispersion. If flavor = 'seurat_v3', ties are broken by the median (across batches) rank based on within-batch normalized variance."/>
+    <param name="never_hvg" argument="--never-hv-genes-file" optional="true" type="data" label="Genes that should never be HVGs" help="Provide a file, one gene identifier per line, with genes that should never be active as HVGs. This simply makes sure that the boolean value for HVGs is false for all these genes after computing HVGs"/>
+    <param name="always_hvg" argument="--always-hv-genes-file" optional="true" type="data" label="Genes that should never be HVGs" help="Provide a file, one gene identifier per line, with genes that should always be active as HVGs. This simply makes sure that the boolean value for HVGs is true for all these genes after computing HVGs"/>
   </inputs>
 
   <outputs>
@@ -77,6 +85,41 @@
          </assert_contents>
       </output>
     </test>
+    <test>
+      <param name="input_obj_file" value="normalise_data.h5"/>
+      <param name="never_hvg" value="never_hvg.txt"/>
+      <param name="always_hvg" value="always_hvg.txt"/>
+      <param name="input_format" value="anndata"/>
+      <param name="output_format" value="anndata"/>
+      <param name="flavor" value="seurat"/>
+      <param name="n_bin" value="20"/>
+      <param name="min_mean" value="0.0125"/>
+      <param name="max_mean" value="3"/>
+      <param name="min_disp" value="0.5"/>
+      <param name="max_disp" value="1e9"/>
+      <output name="output_h5" ftype="h5">
+         <assert_contents>
+           <has_h5_keys keys="uns/hvg"/>
+         </assert_contents>
+      </output>
+    </test>
+    <test>
+      <param name="input_obj_file" value="normalise_data.h5"/>
+      <param name="never_hvg" value="never_hvg.txt"/>
+      <param name="input_format" value="anndata"/>
+      <param name="output_format" value="anndata"/>
+      <param name="flavor" value="seurat"/>
+      <param name="n_bin" value="20"/>
+      <param name="min_mean" value="0.0125"/>
+      <param name="max_mean" value="3"/>
+      <param name="min_disp" value="0.5"/>
+      <param name="max_disp" value="1e9"/>
+      <output name="output_h5" ftype="h5">
+         <assert_contents>
+           <has_h5_keys keys="uns/hvg"/>
+         </assert_contents>
+      </output>
+    </test>
   </tests>
 
   <help><![CDATA[