view scanpy-plot-embedding.xml @ 18:56d22aa6cb3b draft

"planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit 3365d5d4f7930e736fa6814df36d68e578d45d46-dirty"

<?xml version="1.0" encoding="utf-8"?>
<tool id="scanpy_plot_embed" name="Scanpy PlotEmbed" version="@TOOL_VERSION@+galaxy0" profile="@PROFILE@">
  <description>visualise cell embeddings</description>
  <macros>
    <import>scanpy_macros2.xml</import>
  </macros>
  <expand macro="requirements"/>
  <command detect_errors="exit_code"><![CDATA[
ln -s '${input_obj_file}' input.h5 &&
PYTHONIOENCODING=utf-8 scanpy-cli plot embed
@INPUT_OPTS@
#if $color_by
    --color '${color_by}'
#end if
${use_raw}
    --legend-loc '${legend_loc}'
    --legend-fontsize '${legend_fontsize}'
#if $basis
    --basis '${basis}'
#end if
#if $groups
    --groups $groups
#end if
#if $neighbors_key
    --neighbors-key '${neighbors_key}'
#end if    
#if $layer
    --layer '${layer}'
#end if
#if $point_size
    --size ${point_size}
#end if
#if $gene_symbols_field
    --gene-symbols ${gene_symbols_field}
    #end if
${edges} --edges-width ${edges_width}
#if $edges_color
    --edges-color ${edges_color}
#end if
#if $components
    --components ${components}
#end if
--projection $projection
$sort_order
@PLOT_OPTS@
]]></command>

  <inputs>
    <expand macro="input_object_params"/>
    <param name="basis" argument="--basis" type="text" value='umap' label="name of the embedding to plot"/>
    <param name="color_by" argument="--color" type="text" label="color by attributes, comma separated texts">
      <sanitizer>
        <valid initial="string.printable"/>
      </sanitizer>
    </param>
    <param name="groups" argument="--groups" type="text" value="" label="Key for categorical in `.obs`" help="You can pass your predefined groups by choosing any categorical annotation of observations."/>
    <param name="gene_symbols_field" argument="--gene-symbols" type="text" optional="true" label="Field for gene symbols" help="The field used in the AnnData object to store gene symbols, leave blank for the default (index), else a common value is 'gene_symbols'"/>
    <param name="use_raw" argument="--use-raw" type="boolean" checked="true" truevalue="--use-raw" falsevalue="--no-raw" label="Use raw attributes if present"/>
    <param name="layer" argument="--layer" value="" type="text"
        label="Name of the AnnData object layer to plot" help="By default adata.raw.X is plotted. If use_raw=False is set, then adata.X is plotted. If layer is set to a valid layer name, then the layer is plotted. layer takes precedence over use_raw." />
    <param name="neighbors_key" argument="--neighbors-key" value="" type="text"
        label="Where to look for neighbors connectivities." help="If not specified, this looks in .obsp[‘connectivities’] for connectivities (default storage place for pp.neighbors). If specified, this looks .obsp[.uns[neighbors_key][‘connectivities_key’]] for connectivities."/>
    <param name="legend_loc" argument="--legend-loc" type="select" label="Location of legend">
      <option value="right margin" selected="true">Right margin</option>
      <option value="on data">On data</option>
    </param>
    <param name="legend_fontsize" argument="--legend-fontsize" type="integer" value="15" label="Legend font size"/>
    <param name="point_size" argument="--size" type="integer" optional="true" label="Point size"
           help="Automatically determined if not specified."/>
    <param name="edges" argument="--edges" type="boolean" checked="False" truevalue="--edges" falsevalue="" label="Show edges?"/>
    <param name="edges_width" argument="--edges-width" type="float" value="0.1" label="Width of edges"/>
    <param name="edges_color" argument="--edges-color" type="text" value="" label="Color of edges"/>
    <param name="sort_order" argument="--no-sort-order" type="boolean" checked="False" truevalue="" falsevalue="--no-sort-order" label="Plot in order?" help="For continuous annotations used as 'color parameter, plot data points with higher values on top of others."/>
    <param name="components" argument="--components" type="text" value="" label="Components to plot" help="Comma-separated list, e.g. '1,2', '2,3'. To plot all available components use 'all'."/>
    <param name="projection" argument="--projection" type="select" label="Plot projection">
      <option value="2d" selected="true">2D</option>
      <option value="3d">3D</option>
    </param>
    <expand macro="output_plot_params"/>
  </inputs>

  <outputs>
    <data name="output_png" format="png" from_work_dir="output.png" label="${tool.name} on ${on_string}: ${basis} embedding plot"/>
  </outputs>

  <tests>
    <test>
      <param name="input_obj_file" value="find_cluster.h5"/>
      <param name="input_format" value="anndata"/>
      <param name="basis" value="X_pca"/>
      <param name="color_by" value="louvain"/>
      <param name="use_raw" value="false"/>
      <output name="output_png" file="plot_embed.png" ftype="png" compare="sim_size"/>
    </test>
  </tests>

  <help><![CDATA[
=============================================================
Plot embeddings of a given method of dimensionality reduction
=============================================================

It yields a scatter plot in png format, wherein cells are placed in space of
reduced dimensionality and coloured by attribute of choice.

Requires calculating the specified embeddings first. For example, to make
UMAP/TSNE plots, run `Scanpy RunUMAP`/`Scanpy RunTSNE` first, then enter
"umap"/"tsne" as the name of the embedding to plot here.

@HELP@

@VERSION_HISTORY@
]]></help>
  <expand macro="citations"/>
</tool>