Mercurial > repos > ebi-gxa > sccaf_regress_out
diff sccaf_regress_out.xml @ 0:885db50f1bc1 draft
planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/sccaf commit 39b0809db1e17c2a24930fe8fb8ceaf3ea454a7d
author | ebi-gxa |
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date | Tue, 19 Nov 2019 13:06:04 -0500 |
parents | |
children | 5ef0610e9f50 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/sccaf_regress_out.xml Tue Nov 19 13:06:04 2019 -0500 @@ -0,0 +1,45 @@ +<?xml version="1.0" encoding="utf-8"?> +<tool id="sccaf_regress_out" name="SCCAF mulitple regress out" version="@TOOL_VERSION@+galaxy0"> + <description>with multiple categorical keys on an AnnData object.</description> + <macros> + <import>sccaf_macros.xml</import> + </macros> + <expand macro="requirements"/> + <command detect_errors="exit_code"><![CDATA[ + ln -s '${input_obj_file}' input.h5 && + sccaf-regress-out -i input.h5 -o output.h5 -k '${keys_to_regress}' + ]]></command> + <inputs> + <param name="input_obj_file" argument="input-object-file" type="data" format="h5" label="Input object in hdf5 AnnData format"/> + <param name="keys_to_regress" label="Keys to regress" help="Comma separated keys for regressing out; they need to exist in the observations part of the AnnData object." type="text"/> + </inputs> + <outputs> + <data name="output" format="h5" from_work_dir="output.h5" label="${tool.name} on ${on_string}: regressesed out on ${keys_to_regress}"/> + </outputs> + + <tests> + <test> + <param name="input_obj_file" value="find_cluster.h5"/> + <param name="input_format" value="anndata"/> + <param name="color_by" value="louvain"/> + <output name="output" file="output.h5" ftype="h5" compare="sim_size"/> + </test> + </tests> + + <help><![CDATA[ +============================= +Operations on AnnData objects +============================= + +Performs the following operations: + +* Change observation fields, mostly for downstreaming processes convenience. Multiple fields can be changed as one. +* Flag genes that start with a certain text: useful for flagging mitochondrial, spikes or other groups of genes. +* For the flags created, calculates qc metrics (pct_<flag>_counts). +* Calculates `n_genes`, `n_counts` for cells and `n_cells`, `n_counts` for genes. +* For top <N> genes specified, calculate qc metrics (pct_counts_in_top_<N>_genes). + +This functionality will probably be added in the future to a larger package. +]]></help> + <!-- <expand macro="citations"/> --> +</tool>