view seurat_run_pca.xml @ 6:8ded486b0014 draft default tip

planemo upload commit 0264c359f1d638bbbbab515a3502231f679cdcf6

<tool id="seurat_run_pca" name="Seurat RunPCA" profile="18.01" version="@SEURAT_VERSION@+galaxy0">
    <description>run a PCA dimensionality reduction</description>
    <macros>
        <import>seurat_macros.xml</import>
    </macros>
    <expand macro="requirements" />
    <expand macro="version" />
    <command detect_errors="exit_code"><![CDATA[
seurat-run-pca.R

@INPUT_OBJECT@
#if $pc_genes:
    --pc-genes '$pc_genes'
#end if
#if $pc_cells
  --pc-cells '$pc_cells'
#end if
@OUTPUT_OBJECT@
--output-embeddings-file output_embed
--output-loadings-file output_load
--output-stdev-file output_sdev
#if $pcs_compute:
    --pcs-compute '$pcs_compute'
#end if
#if $reverse_pca
  '$reverse_pca'
#end if

]]></command>

    <inputs>
        <expand macro="input_object_params"/>
        <expand macro="output_object_params"/>
        <param name="pc_genes" type="data" format="tabular,txt" optional="True" label="Genes to scale" help="File with gene names to scale/center. Default is all genes in object@data." />
        <param label="Cells to scale" optional="true" name="pc_cells" argument="--pc-cells" type="text" help="File with cell names to scale/center. Default is all cells in object@data."/>
        <param name="pcs_compute" type="integer" optional="True" label="Principal components" help="Total Number of PCs to compute and store (20 by default). Less PCs might be faster, but will explain less variance."/>
        <param label="Reverse PCA" optional="true" name="reverse_pca" argument="--reverse-pca" type="boolean" truevalue="--reverse-pca" checked="false" help="Run PCA on reverse matrix (gene x cell; FALSE by default means cell x gene)."/>
    </inputs>

    <outputs>
        <expand macro="output_files"/>
        <data name="output_embed" format="csv" from_work_dir="output_embed" label="${tool.name} on ${on_string}: Seurat Embeddings"/>
        <data name="output_load" format="csv" from_work_dir="output_load" label="${tool.name} on ${on_string}: Seurat Loadings"/>
        <data name="output_sdev" format="csv" from_work_dir="output_sdev" label="${tool.name} on ${on_string}: Seurat Std dev"/>
    </outputs>

    <tests>
        <test>
            <param name="rds_seurat_file" ftype="rdata" value="E-MTAB-6077-3k_features_90_cells-scaled.rds"/>
            <output name="rds_seurat_file" ftype="rdata">
              <assert_contents>
                <has_size value="5052296" delta="200000"/>
              </assert_contents>
            </output>
            <output name="output_embed" >
              <assert_contents>
                <has_n_lines n="92"/>
              </assert_contents>
            </output>
            <output name="output_load" >
              <assert_contents>
                <has_n_lines n="2001"/>
              </assert_contents>
            </output>
            <output name="output_sdev" >
              <assert_contents>
                <has_n_lines n="50"/>
              </assert_contents>
            </output>
        </test>
    </tests>
    <help><![CDATA[
.. class:: infomark

**What it does**

This tool runs a PCA dimensionality reduction.

@SEURAT_INTRO@

-----

**Inputs**

    * Seurat RDS object, normalised and scaled potentially.
    * Genes used to scale. File of gene names to scale/center. Default is all genes in object.
    * Principal components to compute. Total Number of PCs to compute and store (20 by default). Less PCs might be faster, but will explain less variance.
    * Use imputed. Boolean indicating whether to run PCA on imputed values or not.

-----

**Outputs**

    * Seurat RDS object with PCA calculations and embeddings.
    * Embeddings on CSV file. File with a csv-format embeddings table with principal components by cell.
    * Loadings on CSV file. File with a csv-format loadings table with principal components by gene.
    * Standard deviation on CSV file. Contains principal components std. deviations.

.. _Seurat: https://www.nature.com/articles/nbt.4096
.. _Satija Lab: https://satijalab.org/seurat/

@VERSION_HISTORY@
]]></help>
      <expand macro="citations" />
</tool>