annotate correct_replicates.xml @ 0:607c5e7e0a64 draft

planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/galaxytools-emc/tree/master/tools/mycrobiota commit 1c4c58018b64ff3531a719e789ce71cb0a1244c5

<tool id="mycrobiota-correct-replicates" name="Correct counts" version="0.6">
    <description>in a mothur shared file for replicates and negative controls</description>
    <macros>
        <import>macros.xml</import>
    </macros>
    <command detect_errors="aggressive"><![CDATA[
        python '$__tool_directory__/mycrobiota.py'
            --command correct_replicates
            --shared '$shared'
            --taxonomy '$taxonomy'
            --replicate_suffix '$replicate_suffix'
            --negative_control '$negative_control'
            --nc_copies '$nc_copies'
            --copies '$copies'
            --negative_control_species '$negative_control_species'
    ]]></command>
    <inputs>
        <param argument="--shared" type="data" format="mothur.shared" label="Select shared file"/>
        <param argument="--taxonomy" type="data" format="mothur.ref.taxonomy,mothur.seq.taxonomy,mothur.cons.taxonomy" label="Select taxonomy file"/>
        <param argument="--replicate_suffix" type="text" size="100" value="_replicate" label="Suffix used to indicated replicates" help="e.g. _R if files are named [sample]_R1, [sample]_R2 etc"/>
        <param argument="--negative_control" type="text" optional="True" size="100" value="" label="sample name for your negative control (optional)" help="e.g. negative_control"/>
        <param argument="--negative_control_species" type="text" optional="True" size="100" value="Oscillatoria" label="taxonomy of negative control" help="e.g. Oscillatoria"/>
        <param argument="--nc_copies" type="integer" value="100" min="0" label="number of copies of control species in the negative control sample"/>
        <param argument="--copies" type="integer" value="1000" min="0" label="number of copies of control species in the real samples"/>
    </inputs>
    <outputs>
        <data name="corrected_out" format="mothur.shared" from_work_dir="shared_corrected.tsv" label="${tool.name} on ${on_string}: corrected shared file"/>
        <data name="corrected_tax" format_source="taxonomy" from_work_dir="taxonomy_corrected.tsv" label="${tool.name} on ${on_string}: corrected taxonomy file"/>
        <data name="corrected_out_avg" format="mothur.shared" from_work_dir="shared_averaged.tsv" label="${tool.name} on ${on_string}: averaged only shared file"/>
    </outputs>
    <tests>
        <test>
            <param name="shared" value="shared_tocorrect.tsv" ftype="mothur.shared"/>
            <param name="taxonomy" value="otutable_tocorrect.taxonomy" ftype="mothur.cons.taxonomy"/>
            <param name="replicate_suffix" value="_replicate"/>
            <param name="negative_control" value="NC"/>
            <output name="corrected_out" md5="a7bafe98a383b308b4f1f1681483091d" ftype="mothur.shared"/>
            <output name="corrected_tax" md5="9cc44bec811b868bde9acbe5d857e4c9" ftype="mothur.cons.taxonomy"/>
        </test>
    </tests>
    <help><![CDATA[
**What it does**
averages the counts over all replicates of a sample in a mothur shared file
    ]]></help>
    <expand macro="citations"/>
</tool>