Mercurial > repos > frogs > frogs_3_1_0
annotate test-data/references/01-prepro-flash.log @ 0:59bc96331073 draft default tip
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
author | frogs |
---|---|
date | Thu, 28 Feb 2019 10:14:49 -0500 |
parents | |
children |
rev | line source |
---|---|
0
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
1 ## Application |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
2 Software: preprocess.py (version: 3.1) |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
3 Command: /home/maria/workspace/git/FROGS/FROGS_master/test/../app/preprocess.py illumina --min-amplicon-size 44 --max-amplicon-size 490 --five-prim-primer GGCGVACGGGTGAGTAA --three-prim-primer GTGCCAGCNGCNGCGG --R1-size 267 --R2-size 266 --expected-amplicon-size 420 --merge-software flash --nb-cpus 4 --mismatch-rate 0.15 --keep-unmerged --input-archive /home/maria/workspace/git/FROGS/FROGS-wrapper_dev/test-data/input/temp/test_dataset.tar.gz --output-dereplicated res/01-prepro-flash.fasta --output-count res/01-prepro-flash.tsv --summary res/01-prepro-flash.html --log-file res/01-prepro-flash.log |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
4 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
5 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
6 ##Sample |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
7 R1 : res/1550052675.22_3437_01_R1.fastq |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
8 R2 : res/1550052675.22_3437_01_R2.fastq |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
9 Sample name : 01 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
10 nb seq before process : 30000 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
11 ##Commands |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
12 ######################################################################################################## |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
13 # Join overlapping paired reads. (flash version : v1.2.11) |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
14 Command: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
15 flash --threads 1 --allow-outies --min-overlap 43 --max-overlap 133 --max-mismatch-density 0.15 --compress res/1550052675.22_3437_01_R1.fastq res/1550052675.22_3437_01_R2.fastq --output-directory res --output-prefix 1550052676.19_3438_01_flash 2> res/1550052676.19_3438_01_flash.stderr |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
16 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
17 Execution: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
18 start: 13 Feb 2019 11:11:16 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
19 end: 13 Feb 2019 11:11:22 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
20 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
21 Results: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
22 nb seq paired-end assembled: 17622 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
23 ######################################################################################################## |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
24 # Removes reads without the 5' primer and removes primer sequence. (cutadapt version : 1.18) |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
25 Command: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
26 cutadapt -g GGCGVACGGGTGAGTAA --error-rate 0.1 --discard-untrimmed --match-read-wildcards --overlap 16 -o res/1550052676.19_3438_01_cutadapt_5prim_trim.fastq.gz res/1550052676.19_3438_01_flash.extendedFrags.fastq.gz > res/1550052676.19_3438_01_cutadapt_5prim_log.txt |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
27 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
28 Execution: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
29 start: 13 Feb 2019 11:11:22 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
30 end: 13 Feb 2019 11:11:25 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
31 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
32 Results: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
33 nb seq with 5' primer : 17622 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
34 ######################################################################################################## |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
35 # Removes reads without the 3' primer and removes primer sequence. (cutadapt version : 1.18) |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
36 Command: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
37 cutadapt -a GTGCCAGCNGCNGCGG --error-rate 0.1 --discard-untrimmed --match-read-wildcards --overlap 15 -o res/1550052676.19_3438_01_cutadapt.fastq.gz res/1550052676.19_3438_01_cutadapt_5prim_trim.fastq.gz > res/1550052676.19_3438_01_cutadapt_3prim_log.txt |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
38 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
39 Execution: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
40 start: 13 Feb 2019 11:11:26 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
41 end: 13 Feb 2019 11:11:30 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
42 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
43 Results: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
44 nb seq with 3' primer : 17622 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
45 ######################################################################################################## |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
46 # Filters amplicons without primers by length and N count. (filterSeq.py version : 1.5.0) |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
47 Command: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
48 filterSeq.py --force-fasta --max-N 0 --min-length 11 --max-length 457 --input-file res/1550052676.19_3438_01_cutadapt.fastq.gz --output-file res/1550052676.19_3438_01_N_and_length_filter.fasta --log-file res/1550052676.19_3438_01_N_and_length_filter_log.txt |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
49 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
50 Execution: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
51 start: 13 Feb 2019 11:11:30 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
52 end: 13 Feb 2019 11:11:31 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
53 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
54 Results: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
55 nb seq with expected length : 17622 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
56 nb seq without N : 17622 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
57 ######################################################################################################## |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
58 # Concatenate paired reads. (combine_and_split.py version : ) |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
59 Command: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
60 combine_and_split.py --reads1 res/1550052676.19_3438_01_flash.notCombined_1.fastq.gz --reads2 res/1550052676.19_3438_01_flash.notCombined_2.fastq.gz -c XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX --combined-output res/1550052676.19_3438_01_artificial_combined.fastq.gz |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
61 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
62 Execution: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
63 start: 13 Feb 2019 11:11:31 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
64 end: 13 Feb 2019 11:11:38 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
65 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
66 Results: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
67 nb seq paired-end assembled: 12378 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
68 ######################################################################################################## |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
69 # Removes reads without the 5' primer and removes primer sequence. (cutadapt version : 1.18) |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
70 Command: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
71 cutadapt -g GGCGVACGGGTGAGTAA --error-rate 0.1 --discard-untrimmed --match-read-wildcards --overlap 16 -o res/1550052676.19_3438_01_art_comb_cutadapt_5prim_trim.fastq.gz res/1550052676.19_3438_01_artificial_combined.fastq.gz > res/1550052676.19_3438_01_art_comb_cutadapt_5prim_log.txt |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
72 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
73 Execution: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
74 start: 13 Feb 2019 11:11:39 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
75 end: 13 Feb 2019 11:11:41 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
76 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
77 Results: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
78 nb seq with 5' primer : 12378 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
79 ######################################################################################################## |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
80 # Removes reads without the 3' primer and removes primer sequence. (cutadapt version : 1.18) |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
81 Command: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
82 cutadapt -a GTGCCAGCNGCNGCGG --error-rate 0.1 --discard-untrimmed --match-read-wildcards --overlap 15 -o res/1550052676.19_3438_01_art_comb_cutadapt.fastq.gz res/1550052676.19_3438_01_art_comb_cutadapt_5prim_trim.fastq.gz > res/1550052676.19_3438_01_art_comb_cutadapt_3prim_log.txt |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
83 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
84 Execution: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
85 start: 13 Feb 2019 11:11:42 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
86 end: 13 Feb 2019 11:11:45 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
87 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
88 Results: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
89 nb seq with 3' primer : 12378 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
90 ######################################################################################################## |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
91 # Filters amplicons without primers by length and N count. (filterSeq.py version : 1.5.0) |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
92 Command: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
93 filterSeq.py --force-fasta --max-N 0 --min-length 267 --input-file res/1550052676.19_3438_01_art_comb_cutadapt.fastq.gz --output-file res/1550052676.19_3438_01_art_N_filter.fasta --log-file res/1550052676.19_3438_01_art_N_filter_log.txt |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
94 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
95 Execution: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
96 start: 13 Feb 2019 11:11:45 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
97 end: 13 Feb 2019 11:11:46 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
98 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
99 Results: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
100 nb seq with expected length : 12378 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
101 nb seq without N : 12378 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
102 ######################################################################################################## |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
103 # Replace join tag. (combine_and_split.py version : ) |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
104 Command: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
105 combine_and_split.py --reads1 res/1550052676.19_3438_01_art_N_filter.fasta -s XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX -c NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN --combined-output res/1550052676.19_3438_01_art_XtoN.fasta |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
106 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
107 Execution: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
108 start: 13 Feb 2019 11:11:46 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
109 end: 13 Feb 2019 11:11:50 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
110 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
111 ######################################################################################################## |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
112 # Dereplicates sample sequences. (derepSamples.py version : 1.6.1) |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
113 Command: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
114 derepSamples.py --sequences-files res/1550052676.19_3438_01_N_and_length_filter.fasta res/1550052676.19_3438_01_art_XtoN.fasta --dereplicated-file res/1550052675.22_3437_01_filtered.fasta --count-file res/1550052676.19_3438_01_derep_count.tsv |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
115 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
116 Execution: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
117 start: 13 Feb 2019 11:11:50 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
118 end: 13 Feb 2019 11:11:52 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
119 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
120 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
121 ##Sample |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
122 R1 : res/1550052675.22_3437_02_R1.fastq |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
123 R2 : res/1550052675.22_3437_02_R2.fastq |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
124 Sample name : 02 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
125 nb seq before process : 30000 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
126 ##Commands |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
127 ######################################################################################################## |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
128 # Join overlapping paired reads. (flash version : v1.2.11) |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
129 Command: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
130 flash --threads 1 --allow-outies --min-overlap 43 --max-overlap 133 --max-mismatch-density 0.15 --compress res/1550052675.22_3437_02_R1.fastq res/1550052675.22_3437_02_R2.fastq --output-directory res --output-prefix 1550052676.18_3439_02_flash 2> res/1550052676.18_3439_02_flash.stderr |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
131 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
132 Execution: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
133 start: 13 Feb 2019 11:11:16 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
134 end: 13 Feb 2019 11:11:22 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
135 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
136 Results: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
137 nb seq paired-end assembled: 17500 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
138 ######################################################################################################## |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
139 # Removes reads without the 5' primer and removes primer sequence. (cutadapt version : 1.18) |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
140 Command: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
141 cutadapt -g GGCGVACGGGTGAGTAA --error-rate 0.1 --discard-untrimmed --match-read-wildcards --overlap 16 -o res/1550052676.18_3439_02_cutadapt_5prim_trim.fastq.gz res/1550052676.18_3439_02_flash.extendedFrags.fastq.gz > res/1550052676.18_3439_02_cutadapt_5prim_log.txt |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
142 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
143 Execution: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
144 start: 13 Feb 2019 11:11:22 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
145 end: 13 Feb 2019 11:11:25 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
146 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
147 Results: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
148 nb seq with 5' primer : 17500 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
149 ######################################################################################################## |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
150 # Removes reads without the 3' primer and removes primer sequence. (cutadapt version : 1.18) |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
151 Command: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
152 cutadapt -a GTGCCAGCNGCNGCGG --error-rate 0.1 --discard-untrimmed --match-read-wildcards --overlap 15 -o res/1550052676.18_3439_02_cutadapt.fastq.gz res/1550052676.18_3439_02_cutadapt_5prim_trim.fastq.gz > res/1550052676.18_3439_02_cutadapt_3prim_log.txt |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
153 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
154 Execution: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
155 start: 13 Feb 2019 11:11:26 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
156 end: 13 Feb 2019 11:11:30 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
157 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
158 Results: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
159 nb seq with 3' primer : 17500 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
160 ######################################################################################################## |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
161 # Filters amplicons without primers by length and N count. (filterSeq.py version : 1.5.0) |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
162 Command: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
163 filterSeq.py --force-fasta --max-N 0 --min-length 11 --max-length 457 --input-file res/1550052676.18_3439_02_cutadapt.fastq.gz --output-file res/1550052676.18_3439_02_N_and_length_filter.fasta --log-file res/1550052676.18_3439_02_N_and_length_filter_log.txt |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
164 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
165 Execution: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
166 start: 13 Feb 2019 11:11:30 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
167 end: 13 Feb 2019 11:11:31 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
168 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
169 Results: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
170 nb seq with expected length : 17500 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
171 nb seq without N : 17500 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
172 ######################################################################################################## |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
173 # Concatenate paired reads. (combine_and_split.py version : ) |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
174 Command: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
175 combine_and_split.py --reads1 res/1550052676.18_3439_02_flash.notCombined_1.fastq.gz --reads2 res/1550052676.18_3439_02_flash.notCombined_2.fastq.gz -c XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX --combined-output res/1550052676.18_3439_02_artificial_combined.fastq.gz |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
176 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
177 Execution: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
178 start: 13 Feb 2019 11:11:31 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
179 end: 13 Feb 2019 11:11:38 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
180 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
181 Results: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
182 nb seq paired-end assembled: 12500 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
183 ######################################################################################################## |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
184 # Removes reads without the 5' primer and removes primer sequence. (cutadapt version : 1.18) |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
185 Command: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
186 cutadapt -g GGCGVACGGGTGAGTAA --error-rate 0.1 --discard-untrimmed --match-read-wildcards --overlap 16 -o res/1550052676.18_3439_02_art_comb_cutadapt_5prim_trim.fastq.gz res/1550052676.18_3439_02_artificial_combined.fastq.gz > res/1550052676.18_3439_02_art_comb_cutadapt_5prim_log.txt |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
187 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
188 Execution: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
189 start: 13 Feb 2019 11:11:39 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
190 end: 13 Feb 2019 11:11:42 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
191 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
192 Results: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
193 nb seq with 5' primer : 12500 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
194 ######################################################################################################## |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
195 # Removes reads without the 3' primer and removes primer sequence. (cutadapt version : 1.18) |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
196 Command: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
197 cutadapt -a GTGCCAGCNGCNGCGG --error-rate 0.1 --discard-untrimmed --match-read-wildcards --overlap 15 -o res/1550052676.18_3439_02_art_comb_cutadapt.fastq.gz res/1550052676.18_3439_02_art_comb_cutadapt_5prim_trim.fastq.gz > res/1550052676.18_3439_02_art_comb_cutadapt_3prim_log.txt |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
198 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
199 Execution: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
200 start: 13 Feb 2019 11:11:42 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
201 end: 13 Feb 2019 11:11:45 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
202 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
203 Results: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
204 nb seq with 3' primer : 12500 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
205 ######################################################################################################## |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
206 # Filters amplicons without primers by length and N count. (filterSeq.py version : 1.5.0) |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
207 Command: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
208 filterSeq.py --force-fasta --max-N 0 --min-length 267 --input-file res/1550052676.18_3439_02_art_comb_cutadapt.fastq.gz --output-file res/1550052676.18_3439_02_art_N_filter.fasta --log-file res/1550052676.18_3439_02_art_N_filter_log.txt |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
209 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
210 Execution: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
211 start: 13 Feb 2019 11:11:45 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
212 end: 13 Feb 2019 11:11:46 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
213 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
214 Results: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
215 nb seq with expected length : 12500 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
216 nb seq without N : 12500 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
217 ######################################################################################################## |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
218 # Replace join tag. (combine_and_split.py version : ) |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
219 Command: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
220 combine_and_split.py --reads1 res/1550052676.18_3439_02_art_N_filter.fasta -s XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX -c NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN --combined-output res/1550052676.18_3439_02_art_XtoN.fasta |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
221 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
222 Execution: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
223 start: 13 Feb 2019 11:11:46 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
224 end: 13 Feb 2019 11:11:50 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
225 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
226 ######################################################################################################## |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
227 # Dereplicates sample sequences. (derepSamples.py version : 1.6.1) |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
228 Command: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
229 derepSamples.py --sequences-files res/1550052676.18_3439_02_N_and_length_filter.fasta res/1550052676.18_3439_02_art_XtoN.fasta --dereplicated-file res/1550052675.22_3437_02_filtered.fasta --count-file res/1550052676.18_3439_02_derep_count.tsv |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
230 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
231 Execution: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
232 start: 13 Feb 2019 11:11:51 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
233 end: 13 Feb 2019 11:11:52 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
234 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
235 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
236 ##Sample |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
237 R1 : res/1550052675.22_3437_03_R1.fastq |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
238 R2 : res/1550052675.22_3437_03_R2.fastq |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
239 Sample name : 03 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
240 nb seq before process : 30000 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
241 ##Commands |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
242 ######################################################################################################## |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
243 # Join overlapping paired reads. (flash version : v1.2.11) |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
244 Command: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
245 flash --threads 1 --allow-outies --min-overlap 43 --max-overlap 133 --max-mismatch-density 0.15 --compress res/1550052675.22_3437_03_R1.fastq res/1550052675.22_3437_03_R2.fastq --output-directory res --output-prefix 1550052676.18_3440_03_flash 2> res/1550052676.18_3440_03_flash.stderr |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
246 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
247 Execution: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
248 start: 13 Feb 2019 11:11:16 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
249 end: 13 Feb 2019 11:11:22 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
250 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
251 Results: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
252 nb seq paired-end assembled: 17464 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
253 ######################################################################################################## |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
254 # Removes reads without the 5' primer and removes primer sequence. (cutadapt version : 1.18) |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
255 Command: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
256 cutadapt -g GGCGVACGGGTGAGTAA --error-rate 0.1 --discard-untrimmed --match-read-wildcards --overlap 16 -o res/1550052676.18_3440_03_cutadapt_5prim_trim.fastq.gz res/1550052676.18_3440_03_flash.extendedFrags.fastq.gz > res/1550052676.18_3440_03_cutadapt_5prim_log.txt |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
257 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
258 Execution: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
259 start: 13 Feb 2019 11:11:22 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
260 end: 13 Feb 2019 11:11:24 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
261 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
262 Results: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
263 nb seq with 5' primer : 17464 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
264 ######################################################################################################## |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
265 # Removes reads without the 3' primer and removes primer sequence. (cutadapt version : 1.18) |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
266 Command: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
267 cutadapt -a GTGCCAGCNGCNGCGG --error-rate 0.1 --discard-untrimmed --match-read-wildcards --overlap 15 -o res/1550052676.18_3440_03_cutadapt.fastq.gz res/1550052676.18_3440_03_cutadapt_5prim_trim.fastq.gz > res/1550052676.18_3440_03_cutadapt_3prim_log.txt |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
268 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
269 Execution: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
270 start: 13 Feb 2019 11:11:25 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
271 end: 13 Feb 2019 11:11:28 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
272 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
273 Results: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
274 nb seq with 3' primer : 17464 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
275 ######################################################################################################## |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
276 # Filters amplicons without primers by length and N count. (filterSeq.py version : 1.5.0) |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
277 Command: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
278 filterSeq.py --force-fasta --max-N 0 --min-length 11 --max-length 457 --input-file res/1550052676.18_3440_03_cutadapt.fastq.gz --output-file res/1550052676.18_3440_03_N_and_length_filter.fasta --log-file res/1550052676.18_3440_03_N_and_length_filter_log.txt |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
279 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
280 Execution: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
281 start: 13 Feb 2019 11:11:29 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
282 end: 13 Feb 2019 11:11:30 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
283 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
284 Results: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
285 nb seq with expected length : 17464 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
286 nb seq without N : 17464 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
287 ######################################################################################################## |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
288 # Concatenate paired reads. (combine_and_split.py version : ) |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
289 Command: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
290 combine_and_split.py --reads1 res/1550052676.18_3440_03_flash.notCombined_1.fastq.gz --reads2 res/1550052676.18_3440_03_flash.notCombined_2.fastq.gz -c XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX --combined-output res/1550052676.18_3440_03_artificial_combined.fastq.gz |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
291 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
292 Execution: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
293 start: 13 Feb 2019 11:11:30 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
294 end: 13 Feb 2019 11:11:37 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
295 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
296 Results: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
297 nb seq paired-end assembled: 12536 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
298 ######################################################################################################## |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
299 # Removes reads without the 5' primer and removes primer sequence. (cutadapt version : 1.18) |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
300 Command: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
301 cutadapt -g GGCGVACGGGTGAGTAA --error-rate 0.1 --discard-untrimmed --match-read-wildcards --overlap 16 -o res/1550052676.18_3440_03_art_comb_cutadapt_5prim_trim.fastq.gz res/1550052676.18_3440_03_artificial_combined.fastq.gz > res/1550052676.18_3440_03_art_comb_cutadapt_5prim_log.txt |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
302 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
303 Execution: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
304 start: 13 Feb 2019 11:11:38 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
305 end: 13 Feb 2019 11:11:40 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
306 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
307 Results: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
308 nb seq with 5' primer : 12536 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
309 ######################################################################################################## |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
310 # Removes reads without the 3' primer and removes primer sequence. (cutadapt version : 1.18) |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
311 Command: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
312 cutadapt -a GTGCCAGCNGCNGCGG --error-rate 0.1 --discard-untrimmed --match-read-wildcards --overlap 15 -o res/1550052676.18_3440_03_art_comb_cutadapt.fastq.gz res/1550052676.18_3440_03_art_comb_cutadapt_5prim_trim.fastq.gz > res/1550052676.18_3440_03_art_comb_cutadapt_3prim_log.txt |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
313 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
314 Execution: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
315 start: 13 Feb 2019 11:11:41 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
316 end: 13 Feb 2019 11:11:43 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
317 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
318 Results: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
319 nb seq with 3' primer : 12536 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
320 ######################################################################################################## |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
321 # Filters amplicons without primers by length and N count. (filterSeq.py version : 1.5.0) |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
322 Command: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
323 filterSeq.py --force-fasta --max-N 0 --min-length 267 --input-file res/1550052676.18_3440_03_art_comb_cutadapt.fastq.gz --output-file res/1550052676.18_3440_03_art_N_filter.fasta --log-file res/1550052676.18_3440_03_art_N_filter_log.txt |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
324 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
325 Execution: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
326 start: 13 Feb 2019 11:11:44 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
327 end: 13 Feb 2019 11:11:45 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
328 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
329 Results: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
330 nb seq with expected length : 12536 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
331 nb seq without N : 12536 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
332 ######################################################################################################## |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
333 # Replace join tag. (combine_and_split.py version : ) |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
334 Command: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
335 combine_and_split.py --reads1 res/1550052676.18_3440_03_art_N_filter.fasta -s XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX -c NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN --combined-output res/1550052676.18_3440_03_art_XtoN.fasta |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
336 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
337 Execution: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
338 start: 13 Feb 2019 11:11:45 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
339 end: 13 Feb 2019 11:11:49 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
340 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
341 ######################################################################################################## |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
342 # Dereplicates sample sequences. (derepSamples.py version : 1.6.1) |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
343 Command: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
344 derepSamples.py --sequences-files res/1550052676.18_3440_03_N_and_length_filter.fasta res/1550052676.18_3440_03_art_XtoN.fasta --dereplicated-file res/1550052675.22_3437_03_filtered.fasta --count-file res/1550052676.18_3440_03_derep_count.tsv |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
345 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
346 Execution: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
347 start: 13 Feb 2019 11:11:49 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
348 end: 13 Feb 2019 11:11:51 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
349 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
350 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
351 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
352 ##Sample |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
353 All |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
354 ##Commands |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
355 ######################################################################################################## |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
356 # Dereplicates together sequences from several samples. (derepSamples.py version : 1.6.1) |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
357 Command: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
358 derepSamples.py --nb-cpus 4 --size-separator ';size=' --samples-ref res/1550052675.22_3437_derep_inputs.tsv --dereplicated-file res/01-prepro-flash.fasta --count-file res/01-prepro-flash.tsv |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
359 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
360 Execution: |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
361 start: 13 Feb 2019 11:11:52 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
362 end: 13 Feb 2019 11:11:53 |
59bc96331073
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
frogs
parents:
diff
changeset
|
363 |