Mercurial > repos > galaxyp > msi_filtering
annotate msi_filtering.xml @ 2:22db5eb94e50 draft
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 1c808d60243bb1eeda0cd26cb4b0a17ab05de2c0
author | galaxyp |
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date | Mon, 28 May 2018 12:36:24 -0400 |
parents | 98c101b19f3c |
children | d51c3c814d57 |
rev | line source |
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2
22db5eb94e50
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 1c808d60243bb1eeda0cd26cb4b0a17ab05de2c0
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1 <tool id="mass_spectrometry_imaging_filtering" name="MSI filtering" version="1.10.0.0"> |
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f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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2 <description>tool for filtering mass spectrometry imaging data</description> |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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3 <requirements> |
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22db5eb94e50
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 1c808d60243bb1eeda0cd26cb4b0a17ab05de2c0
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4 <requirement type="package" version="1.10.0">bioconductor-cardinal</requirement> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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5 <requirement type="package" version="2.2.1">r-gridextra</requirement> |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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6 </requirements> |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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7 <command detect_errors="exit_code"> |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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8 <![CDATA[ |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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9 |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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10 #if $infile.ext == 'imzml' |
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22db5eb94e50
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 1c808d60243bb1eeda0cd26cb4b0a17ab05de2c0
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11 ln -s '${infile.extra_files_path}/imzml' infile.imzML && |
22db5eb94e50
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 1c808d60243bb1eeda0cd26cb4b0a17ab05de2c0
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12 ln -s '${infile.extra_files_path}/ibd' infile.ibd && |
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f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
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13 #elif $infile.ext == 'analyze75' |
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22db5eb94e50
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 1c808d60243bb1eeda0cd26cb4b0a17ab05de2c0
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14 ln -s '${infile.extra_files_path}/hdr' infile.hdr && |
22db5eb94e50
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 1c808d60243bb1eeda0cd26cb4b0a17ab05de2c0
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15 ln -s '${infile.extra_files_path}/img' infile.img && |
22db5eb94e50
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 1c808d60243bb1eeda0cd26cb4b0a17ab05de2c0
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16 ln -s '${infile.extra_files_path}/t2m' infile.t2m && |
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f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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17 #else |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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18 ln -s $infile infile.RData && |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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19 #end if |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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20 cat '${MSI_subsetting}' && |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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21 echo ${MSI_subsetting} && |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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22 Rscript '${MSI_subsetting}' |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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23 |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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24 ]]> |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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25 </command> |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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26 <configfiles> |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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27 <configfile name="MSI_subsetting"><![CDATA[ |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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28 |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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29 |
2
22db5eb94e50
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 1c808d60243bb1eeda0cd26cb4b0a17ab05de2c0
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30 ################################# load libraries and read file ################# |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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31 |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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32 |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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33 library(Cardinal) |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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34 library(gridExtra) |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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35 |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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36 #if $infile.ext == 'imzml' |
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22db5eb94e50
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 1c808d60243bb1eeda0cd26cb4b0a17ab05de2c0
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37 msidata = readImzML('infile') |
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f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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38 #elif $infile.ext == 'analyze75' |
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22db5eb94e50
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 1c808d60243bb1eeda0cd26cb4b0a17ab05de2c0
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39 msidata = readAnalyze('infile') |
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f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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40 #else |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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41 load('infile.RData') |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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42 #end if |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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43 |
2
22db5eb94e50
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 1c808d60243bb1eeda0cd26cb4b0a17ab05de2c0
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44 ##################################### QC: inputfile properties in numbers ###### |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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45 |
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98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
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46 #if $outputs.outputs_select == "quality_control": |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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47 ## Number of features (mz) |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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48 maxfeatures = length(features(msidata)) |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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49 ## Range mz |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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50 minmz = round(min(mz(msidata)), digits=2) |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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51 maxmz = round(max(mz(msidata)), digits=2) |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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52 ## Number of spectra (pixels) |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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53 pixelcount = length(pixels(msidata)) |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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54 ## Range x coordinates |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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55 minimumx = min(coord(msidata)[,1]) |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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56 maximumx = max(coord(msidata)[,1]) |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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57 ## Range y coordinates |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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58 minimumy = min(coord(msidata)[,2]) |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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59 maximumy = max(coord(msidata)[,2]) |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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60 ## Number of intensities > 0 |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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61 npeaks= sum(spectra(msidata)[]>0) |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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62 ## Spectra multiplied with mz (potential number of peaks) |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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63 numpeaks = ncol(spectra(msidata)[])*nrow(spectra(msidata)[]) |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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64 ## Percentage of intensities > 0 |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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65 percpeaks = round(npeaks/numpeaks*100, digits=2) |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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66 ## Number of empty TICs |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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67 TICs = colSums(spectra(msidata)[]) |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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68 NumemptyTIC = sum(TICs == 0) |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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69 ## median TIC |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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70 medint = round(median(TICs), digits=2) |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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71 ## Store features for QC plot |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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72 featuresinfile = mz(msidata) |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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73 #end if |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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74 |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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75 |
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planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 1c808d60243bb1eeda0cd26cb4b0a17ab05de2c0
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76 ###################################### Filtering of pixels ##################### |
22db5eb94e50
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 1c808d60243bb1eeda0cd26cb4b0a17ab05de2c0
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77 |
22db5eb94e50
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 1c808d60243bb1eeda0cd26cb4b0a17ab05de2c0
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78 ### Pixels in the one column format "x=,y=" |
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98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
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79 |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
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80 #if str($pixels_cond.pixel_filtering) == "single_column": |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
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81 print("single column") |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
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82 |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
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83 input_list = read.delim("$pixels_cond.single_pixels", header = FALSE, stringsAsFactors = FALSE) |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
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84 numberpixels = length(input_list[,$pixels_cond.pixel_column]) |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
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85 valid_entries = input_list[,$pixels_cond.pixel_column] %in% names(pixels(msidata)) |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
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86 validpixels = sum(valid_entries) |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
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87 |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
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88 if (validpixels != 0) |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
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89 { |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
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90 pixelsofinterest = pixels(msidata)[names(pixels(msidata)) %in% input_list[valid_entries,$pixels_cond.pixel_column]] |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
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91 msidata = msidata[,pixelsofinterest] |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
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92 }else{ |
2
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93 msidata = msidata[,0] |
1
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94 validpixels=0 |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
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95 } |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
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96 |
2
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97 |
22db5eb94e50
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 1c808d60243bb1eeda0cd26cb4b0a17ab05de2c0
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98 ### Pixels in two columns format: x and y in different columns |
1
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99 |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
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100 #elif str($pixels_cond.pixel_filtering) == "two_columns": |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
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101 print("two columns") |
0
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102 |
1
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103 input_list = read.delim("$pixels_cond.two_columns_pixel", header = FALSE, |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
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104 stringsAsFactors = FALSE) |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
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105 numberpixels = length(input_list[,$pixels_cond.pixel_column_x]) |
98c101b19f3c
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106 |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
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107 inputpixel_x = input_list[,$pixels_cond.pixel_column_x] |
98c101b19f3c
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108 inputpixel_y = input_list[,$pixels_cond.pixel_column_y] |
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109 |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
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110 inputpixels = cbind(inputpixel_x, inputpixel_y) |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
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111 colnames(inputpixels) = c("x", "y") |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
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112 valid_rows = merge(inputpixels, coord(msidata)[,1:2]) |
98c101b19f3c
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113 validpixels = nrow(valid_rows) |
98c101b19f3c
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114 |
98c101b19f3c
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115 if (validpixels != 0) |
0
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116 { |
1
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117 |
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118 pixelvector = character() |
98c101b19f3c
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119 |
98c101b19f3c
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120 for (pixel in 1:nrow(valid_rows)) |
98c101b19f3c
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121 { |
98c101b19f3c
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122 pixelvector[pixel] = paste0("x = ", valid_rows[pixel,1],", ", "y = ", valid_rows[pixel,2]) |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
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123 } |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
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124 |
98c101b19f3c
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125 pixelsofinterest= pixels(msidata)[names(pixels(msidata)) %in% pixelvector] |
0
f17d3f1a065f
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126 msidata = msidata[,pixelsofinterest] |
1
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127 }else{ |
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128 validpixels=0 |
0
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129 } |
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130 |
2
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131 |
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132 ### Pixels wihin x and y minima and maxima are kept: |
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133 |
1
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134 #elif str($pixels_cond.pixel_filtering) == "pixel_range": |
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135 print("pixel range") |
98c101b19f3c
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136 |
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137 numberpixels = "range" |
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138 validpixels = "range" |
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139 |
2
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140 ## only filter pixels if at least one pixel will be left |
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141 |
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142 if (sum(coord(msidata)\$x <= $pixels_cond.max_x_range & coord(msidata)\$x >= $pixels_cond.min_x_range) > 0 & sum(coord(msidata)\$y <= $pixels_cond.max_y_range & coord(msidata)\$y >= $pixels_cond.min_y_range) > 0) |
1
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143 { |
98c101b19f3c
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144 msidata = msidata[, coord(msidata)\$x <= $pixels_cond.max_x_range & coord(msidata)\$x >= $pixels_cond.min_x_range] |
98c101b19f3c
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145 msidata = msidata[, coord(msidata)\$y <= $pixels_cond.max_y_range & coord(msidata)\$y >= $pixels_cond.min_y_range] |
2
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146 }else{ |
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147 msidata = msidata[,0] |
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148 print("no valid pixel found") |
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149 } |
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150 |
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151 |
98c101b19f3c
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152 |
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153 #elif str($pixels_cond.pixel_filtering) == "none": |
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154 print("no pixel filtering") |
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155 numberpixels = 0 |
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156 validpixels = 0 |
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157 |
0
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158 #end if |
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159 |
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160 |
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161 |
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162 ###################################### filtering of features ###################### |
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163 |
2
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164 ### Tabular file contains mz either as numbers or in the format mz=800.01 |
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165 |
1
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166 #if str($features_cond.features_filtering) == "features_list": |
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167 print("feature list") |
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168 |
1
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169 input_features = read.delim("$inputfeatures", header = FALSE, stringsAsFactors = FALSE) |
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170 |
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171 startingrow = $features_cond.feature_header+1 |
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172 extracted_features = input_features[startingrow:nrow(input_features),$features_cond.feature_column] |
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173 numberfeatures = length(extracted_features) |
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174 |
1
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175 if (grepl("m/z = ", input_features[startingrow,$features_cond.feature_column])==FALSE) |
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176 |
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177 ### if input is in numeric format |
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178 { |
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179 |
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180 if (class(extracted_features) == "numeric") |
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181 { |
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182 charactervector = rep("m/z = ", numberfeatures) |
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183 mz_added = paste0(charactervector, round(extracted_features,digits=2)) |
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184 validfeatures = mz_added %in% names(features(msidata)) |
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185 featuresofinterest = features(msidata)[names(features(msidata)) %in% mz_added[validfeatures]] |
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186 validmz = sum(validfeatures) |
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187 }else{ |
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188 validmz = 0 |
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189 featuresofinterest = 0 |
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190 } |
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191 |
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192 ### if input is already in character format (m/z = 800.01) |
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193 |
1
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194 }else{ |
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195 validfeatures = extracted_features %in% names(features(msidata)) |
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196 featuresofinterest = features(msidata)[names(features(msidata)) %in% extracted_features[validfeatures]] |
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197 validmz = sum(validfeatures) |
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198 } |
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199 |
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200 ### filter msidata for valid features |
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201 msidata = msidata[featuresofinterest,] |
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202 |
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203 |
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204 ### Only features within a given minimum and maximum value are kept: |
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205 |
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206 #elif str($features_cond.features_filtering) == "features_range": |
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207 print("feature range") |
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208 |
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209 numberfeatures = "range" |
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210 validmz = "range" |
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211 |
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212 if (sum(mz(msidata) >= $features_cond.min_mz & mz(msidata) <= $features_cond.max_mz)> 0) |
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213 { |
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214 msidata = msidata[mz(msidata) >= $features_cond.min_mz & mz(msidata) <= $features_cond.max_mz,] |
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215 }else{ |
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216 msidata = msidata[0,] |
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217 print("no valid mz range") |
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218 } |
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219 |
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220 |
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221 #elif str($features_cond.features_filtering) == "none": |
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222 |
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223 print("no feature filtering") |
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224 validmz = 0 |
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225 numberfeatures = 0 |
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226 #end if |
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227 |
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228 |
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229 |
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230 # save msidata as Rfile |
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231 save(msidata, file="$msidata_filtered") |
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232 |
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233 ###################################### outputfile properties in numbers ######## |
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234 |
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planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
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235 #if $outputs.outputs_select == "quality_control": |
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236 |
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237 ## Number of features (mz) |
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238 maxfeatures2 = length(features(msidata)) |
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239 ## Range mz |
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240 minmz2 = round(min(mz(msidata)), digits=2) |
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241 maxmz2 = round(max(mz(msidata)), digits=2) |
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242 ## Number of spectra (pixels) |
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243 pixelcount2 = length(pixels(msidata)) |
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244 ## Range x coordinates |
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245 minimumx2 = min(coord(msidata)[,1]) |
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246 maximumx2 = max(coord(msidata)[,1]) |
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247 ## Range y coordinates |
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248 minimumy2 = min(coord(msidata)[,2]) |
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249 maximumy2 = max(coord(msidata)[,2]) |
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250 ## Number of intensities > 0 |
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251 npeaks2= sum(spectra(msidata)[]>0) |
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252 ## Spectra multiplied with mz (potential number of peaks) |
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253 numpeaks2 = ncol(spectra(msidata)[])*nrow(spectra(msidata)[]) |
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254 ## Percentage of intensities > 0 |
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255 percpeaks2 = round(npeaks2/numpeaks2*100, digits=2) |
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256 ## Number of empty TICs |
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257 TICs2 = colSums(spectra(msidata)[]) |
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258 NumemptyTIC2 = sum(TICs2 == 0) |
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259 ## median TIC |
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260 medint2 = round(median(TICs2), digits=2) |
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261 |
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262 |
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263 properties = c("Number of mz features", |
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264 "Range of mz values [Da]", |
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265 "Number of pixels", |
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266 "Range of x coordinates", |
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267 "Range of y coordinates", |
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268 "Intensities > 0", |
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269 "Median TIC per pixel", |
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270 "Number of zero TICs", |
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271 "pixel overview", |
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272 "feature overview") |
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273 |
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274 before = c(paste0(maxfeatures), |
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275 paste0(minmz, " - ", maxmz), |
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276 paste0(pixelcount), |
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277 paste0(minimumx, " - ", maximumx), |
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278 paste0(minimumy, " - ", maximumy), |
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279 paste0(percpeaks, " %"), |
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280 paste0(medint), |
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281 paste0(NumemptyTIC), |
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282 paste0("input pixels: ", numberpixels), |
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283 paste0("input mz: ", numberfeatures)) |
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284 |
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285 filtered = c(paste0(maxfeatures2), |
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286 paste0(minmz2, " - ", maxmz2), |
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287 paste0(pixelcount2), |
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288 paste0(minimumx2, " - ", maximumx2), |
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289 paste0(minimumy2, " - ", maximumy2), |
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290 paste0(percpeaks2, " %"), |
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291 paste0(medint2), |
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292 paste0(NumemptyTIC2), |
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293 paste0("valid pixels: ", validpixels), |
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294 paste0("valid mz: ", validmz)) |
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295 |
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296 |
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297 property_df = data.frame(properties, before, filtered) |
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298 |
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299 |
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300 |
2
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301 ######################################## PDF QC ################################ |
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302 |
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303 pdf("filtertool_QC.pdf", fonts = "Times", pointsize = 12) |
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304 plot(0,type='n',axes=FALSE,ann=FALSE) |
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305 |
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306 title(main=paste0("Qualitycontrol of filtering tool for file: \n\n", "$infile.display_name")) |
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307 |
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308 |
1
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309 |
0
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310 grid.table(property_df, rows= NULL) |
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311 |
1
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planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
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312 ### heatmap image as visual pixel control |
0
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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313 |
1
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
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314 if (length(features(msidata))> 0 & length(pixels(msidata)) > 0) |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
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315 { |
0
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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316 |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
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317 |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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318 image(msidata, mz=$outputs.inputmz, plusminus = $outputs.plusminus_dalton, contrast.enhance = "none", |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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319 main= paste0($outputs.inputmz," ± ", $outputs.plusminus_dalton, " Da"), ylim = c(maximumy2+0.2*maximumy2,minimumy2-0.2*minimumy2)) |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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320 |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
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321 ### control features which are left |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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322 |
1
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
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323 plot(featuresinfile, rep(1,length(featuresinfile)), yaxt="n", ylab=NA, xlab="m/z values", col="red", ylim=c(0.8, 1.1), main="Distribution of m/z values") |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
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324 lines(mz(msidata),rep(0.9, length(mz(msidata))), col="green", type="p") |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
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325 legend("top", horiz=TRUE, |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
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326 legend = c("before", "filtered"), |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
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327 fill = c("red", "green")) |
0
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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328 |
1
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
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329 }else{ |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
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330 print("file has no features or pixels left") |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
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331 } |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
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332 |
0
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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333 dev.off() |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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334 |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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335 #end if |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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336 |
2
22db5eb94e50
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 1c808d60243bb1eeda0cd26cb4b0a17ab05de2c0
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337 ######################################## intensity matrix ###################### |
0
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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338 |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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339 #if $output_matrix: |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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340 |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
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341 if (length(features(msidata))> 0 & length(pixels(msidata)) > 0) |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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342 { |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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343 |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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344 spectramatrix = spectra(msidata) |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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345 rownames(spectramatrix) = mz(msidata) |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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346 newmatrix = rbind(pixels(msidata), spectramatrix) |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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347 write.table(newmatrix[2:nrow(newmatrix),], file="$matrixasoutput", quote = FALSE, row.names = TRUE, col.names=NA, sep = "\t") |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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348 |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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349 }else{ |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
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350 print("file has no features or pixels left") |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
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351 } |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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352 |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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353 #end if |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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354 |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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355 |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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356 ]]></configfile> |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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357 </configfiles> |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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358 <inputs> |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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359 <param name="infile" type="data" format="imzml, rdata, analyze75" |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
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parents:
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360 label="Inputfile as imzML, Analyze7.5 or Cardinal MSImageSet saved as RData" |
1
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
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361 help="Upload composite datatype imzML (ibd+imzML) or analyze75 (hdr+img+t2m) or regular upload .RData (Cardinal MSImageSet)"/> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
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362 <conditional name="pixels_cond"> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
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363 <param name="pixel_filtering" type="select" label="Select pixel filtering option"> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
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364 <option value="none" selected="True">none</option> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
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365 <option value="single_column">tabular file with single column (x = 1, y = 1)</option> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
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366 <option value="two_columns">tabular file with separate columns for x and y values</option> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
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367 <option value="pixel_range">ranges for x and y</option> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
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368 </param> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
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369 <when value="none"/> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
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370 <when value="single_column"> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
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371 <param name="single_pixels" type="data" format="tabular" label="Pixels in single column for filtering of MSI data" |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
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372 help="tabular file with pixels of interest in the form x = 1, y = 1"/> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
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373 <param name="pixel_column" data_ref="single_pixels" label="Column with pixels" type="data_column"/> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
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374 </when> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
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375 <when value="two_columns"> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
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376 <param name="two_columns_pixel" type="data" format="tabular" label="Pixels in two columns for filtering of MSI data" |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
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377 help="tabular file with pixels of interest in two separate columns"/> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
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378 <param name="pixel_column_x" data_ref="two_columns_pixel" label="Column with x values" type="data_column"/> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
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379 <param name="pixel_column_y" data_ref="two_columns_pixel" label="Column with y values" type="data_column"/> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
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380 </when> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
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381 <when value="pixel_range"> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
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382 <param name="min_x_range" type="integer" value="0" label="Minimum value for x"/> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
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diff
changeset
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383 <param name="max_x_range" type="integer" value="100" label="Maximum value for x"/> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
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diff
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384 <param name="min_y_range" type="integer" value="0" label="Minimum value for y"/> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
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diff
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385 <param name="max_y_range" type="integer" value="100" label="Maximum value for y"/> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
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386 </when> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
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changeset
|
387 </conditional> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
388 <conditional name="features_cond"> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
389 <param name="features_filtering" type="select" label="Select feature filtering option"> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
390 <option value="none" selected="True">none</option> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
391 <option value="features_list">tabular file with features (data type: 800.12 or m/z = 800.12)</option> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
392 <option value="features_range">range of features</option> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
393 </param> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
394 <when value="none"/> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
395 <when value="features_list"> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
396 <param name="inputfeatures" type="data" format="tabular" label="Features for filtering of MSI data" help="tabular file with masses of interest either as numbers (800.05) or in the form m/z = 800.05"/> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
397 <param name="feature_column" data_ref="inputfeatures" label="Column with features" type="data_column"/> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
398 <param name="feature_header" label="Number of header lines to skip" value="0" type="integer"/> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
399 </when> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
400 <when value="features_range"> |
2
22db5eb94e50
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 1c808d60243bb1eeda0cd26cb4b0a17ab05de2c0
galaxyp
parents:
1
diff
changeset
|
401 <param name="min_mz" type="float" value="1" label="Minimum value for mz (in Dalton)"/> |
22db5eb94e50
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 1c808d60243bb1eeda0cd26cb4b0a17ab05de2c0
galaxyp
parents:
1
diff
changeset
|
402 <param name="max_mz" type="float" value="100" label="Maximum value for mz (in Dalton)"/> |
1
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
403 </when> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
404 </conditional> |
0
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
405 <conditional name="outputs"> |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
406 <param name="outputs_select" type="select" label="Quality control output"> |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
407 <option value="quality_control" selected="True">yes</option> |
1
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
408 <option value="no_quality_control">no</option> |
0
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
409 </param> |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
410 <when value="quality_control"> |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
411 <param name="inputmz" type="float" value="1296.7" label="Mass for which a heatmap image will be drawn" help="Use a mass which is still present in all pixels to control if the pixel filtering went well"/> |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
412 <param name="plusminus_dalton" value="0.25" type="float" label="mass range for mz value" help="plusminus mass window in Dalton"/> |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
413 </when> |
1
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
414 <when value="no_quality_control"/> |
0
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
415 </conditional> |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
416 <param name="output_matrix" type="boolean" display="radio" label="Intensity matrix output"/> |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
417 </inputs> |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
418 <outputs> |
1
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
419 <data format="rdata" name="msidata_filtered" label="${tool.name} ${on_string}"/> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
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diff
changeset
|
420 <data format="pdf" name="filtering_qc" from_work_dir="filtertool_QC.pdf" label = "QC ${tool.name} ${on_string}"> |
0
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
421 <filter>outputs["outputs_select"] == "quality_control"</filter> |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
422 </data> |
2
22db5eb94e50
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 1c808d60243bb1eeda0cd26cb4b0a17ab05de2c0
galaxyp
parents:
1
diff
changeset
|
423 <data format="tabular" name="matrixasoutput" label="Matrix ${tool.name} ${on_string}"> |
0
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
424 <filter>output_matrix</filter> |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
425 </data> |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
426 </outputs> |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
427 <tests> |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
428 <test expect_num_outputs="2"> |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
429 <param name="infile" value="" ftype="imzml"> |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
430 <composite_data value="Example_Continuous.imzML"/> |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
431 <composite_data value="Example_Continuous.ibd"/> |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
432 </param> |
1
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
433 <param name="pixel_filtering" value="single_column"/> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
434 <param name="single_pixels" ftype="tabular" value = "inputpixels.tabular"/> |
0
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
435 <param name="pixel_column" value="1"/> |
1
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
436 <param name="features_filtering" value="features_list"/> |
0
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
437 <param name="inputfeatures" ftype="tabular" value = "inputfeatures.tabular"/> |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
438 <param name="feature_column" value="2"/> |
1
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
439 <param name="feature_header" value="1"/> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
440 <param name="outputs_select" value="quality_control"/> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
441 <param name="inputmz" value="328.9"/> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
442 <param name="plusminus_dalton" value="0.25"/> |
0
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
443 <output name="filtering_qc" file="imzml_filtered.pdf" compare="sim_size" delta="20000"/> |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
444 <output name="msidata_filtered" file="imzml_filtered.RData" compare="sim_size" /> |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
445 </test> |
1
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
446 <test expect_num_outputs="2"> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
447 <param name="infile" value="" ftype="imzml"> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
448 <composite_data value="Example_Continuous.imzML"/> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
449 <composite_data value="Example_Continuous.ibd"/> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
450 </param> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
451 <param name="pixel_filtering" value="pixel_range"/> |
2
22db5eb94e50
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 1c808d60243bb1eeda0cd26cb4b0a17ab05de2c0
galaxyp
parents:
1
diff
changeset
|
452 <param name="min_x_range" value="10"/> |
22db5eb94e50
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 1c808d60243bb1eeda0cd26cb4b0a17ab05de2c0
galaxyp
parents:
1
diff
changeset
|
453 <param name="max_x_range" value="20"/> |
1
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
454 <param name="min_y_range" value="2"/> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
455 <param name="max_y_range" value="2"/> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
456 <param name="outputs_select" value="quality_control"/> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
457 <param name="inputmz" value="328.9"/> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
458 <param name="plusminus_dalton" value="0.25"/> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
459 <output name="filtering_qc" file="imzml_filtered2.pdf" compare="sim_size" delta="20000"/> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
460 <output name="msidata_filtered" file="imzml_filtered2.RData" compare="sim_size" /> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
461 </test> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
462 <test expect_num_outputs="3"> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
463 <param name="infile" value="" ftype="imzml"> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
464 <composite_data value="Example_Continuous.imzML"/> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
465 <composite_data value="Example_Continuous.ibd"/> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
466 </param> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
467 <param name="pixel_filtering" value="pixel_range"/> |
2
22db5eb94e50
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 1c808d60243bb1eeda0cd26cb4b0a17ab05de2c0
galaxyp
parents:
1
diff
changeset
|
468 <param name="min_x_range" value="1"/> |
22db5eb94e50
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 1c808d60243bb1eeda0cd26cb4b0a17ab05de2c0
galaxyp
parents:
1
diff
changeset
|
469 <param name="max_x_range" value="20"/> |
1
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
470 <param name="min_y_range" value="2"/> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
471 <param name="max_y_range" value="2"/> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
472 <param name="features_filtering" value="features_range"/> |
2
22db5eb94e50
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 1c808d60243bb1eeda0cd26cb4b0a17ab05de2c0
galaxyp
parents:
1
diff
changeset
|
473 <param name="min_mz" value="0" /> |
1
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
474 <param name="max_mz" value="500"/> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
475 <param name="outputs_select" value="quality_control"/> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
476 <param name="inputmz" value="328.9"/> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
477 <param name="plusminus_dalton" value="0.25"/> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
478 <param name="output_matrix" value="True"/> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
479 <output name="filtering_qc" file="imzml_filtered3.pdf" compare="sim_size" delta="20000"/> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
480 <output name="msidata_filtered" file="imzml_filtered3.RData" compare="sim_size" /> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
481 <output name="matrixasoutput" file="imzml_matrix3.tabular"/> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
482 </test> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
483 <test expect_num_outputs="2"> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
484 <param name="infile" value="" ftype="imzml"> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
485 <composite_data value="Example_Continuous.imzML"/> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
486 <composite_data value="Example_Continuous.ibd"/> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
487 </param> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
488 <param name="pixel_filtering" value="two_columns"/> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
489 <param name="two_columns_pixel" ftype="tabular" value = "inputpixels_2column.tabular"/> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
490 <param name="pixel_column_x" value="1"/> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
491 <param name="pixel_column_y" value="3"/> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
492 <param name="features_filtering" value="features_list"/> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
493 <param name="inputfeatures" ftype="tabular" value = "inputcalibrantfile2.txt"/> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
494 <param name="feature_column" value="1"/> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
495 <param name="feature_header" value="0"/> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
496 <param name="outputs_select" value="quality_control"/> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
497 <param name="inputmz" value="328.9"/> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
498 <param name="plusminus_dalton" value="0.25"/> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
499 <output name="filtering_qc" file="imzml_filtered4.pdf" compare="sim_size" delta="20000"/> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
500 <output name="msidata_filtered" file="imzml_filtered4.RData" compare="sim_size" /> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
501 </test> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
502 <test expect_num_outputs="2"> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
503 <param name="infile" value="" ftype="imzml"> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
504 <composite_data value="Example_Continuous.imzML"/> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
505 <composite_data value="Example_Continuous.ibd"/> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
506 </param> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
507 <param name="pixel_filtering" value="pixel_range"/> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
508 <param name="min_x_range" value="0"/> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
509 <param name="max_x_range" value="10"/> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
510 <param name="min_y_range" value="2"/> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
511 <param name="max_y_range" value="20"/> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
512 <param name="features_filtering" value="features_range"/> |
2
22db5eb94e50
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 1c808d60243bb1eeda0cd26cb4b0a17ab05de2c0
galaxyp
parents:
1
diff
changeset
|
513 <param name="min_mz" value="500" /> |
22db5eb94e50
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 1c808d60243bb1eeda0cd26cb4b0a17ab05de2c0
galaxyp
parents:
1
diff
changeset
|
514 <param name="max_mz" value="700"/> |
1
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
515 <param name="outputs_select" value="quality_control"/> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
516 <param name="inputmz" value="328.9"/> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
517 <param name="plusminus_dalton" value="0.25"/> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
518 <output name="filtering_qc" file="imzml_filtered5.pdf" compare="sim_size" delta="20000"/> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
519 <output name="msidata_filtered" file="imzml_filtered5.RData" compare="sim_size" /> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
520 </test> |
0
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
521 <test expect_num_outputs="3"> |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
522 <param name="infile" value="" ftype="analyze75"> |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
523 <composite_data value="Analyze75.hdr"/> |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
524 <composite_data value="Analyze75.img"/> |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
525 <composite_data value="Analyze75.t2m"/> |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
526 </param> |
1
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
527 <param name="pixel_filtering" value="single_column"/> |
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
528 <param name="single_pixels" ftype="tabular" value = "inputpixels2.tabular"/> |
0
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
529 <param name="pixel_column" value="1"/> |
1
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
530 <param name="features_filtering" value="features_list"/> |
0
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
531 <param name="inputfeatures" ftype="tabular" value = "featuresofinterest2.tabular"/> |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
532 <param name="feature_column" value="1"/> |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
533 <conditional name="outputs"> |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
534 <param name="outputs_select" value="quality_control"/> |
2
22db5eb94e50
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 1c808d60243bb1eeda0cd26cb4b0a17ab05de2c0
galaxyp
parents:
1
diff
changeset
|
535 <param name="inputmz" value="1200"/> |
0
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
536 <param name="plusminus_dalton" value="0.25"/> |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
537 </conditional> |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
538 <param name="output_matrix" value="True"/> |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
539 <output name="filtering_qc" file="analyze_filtered.pdf" compare="sim_size" delta="20000"/> |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
540 <output name="msidata_filtered" file="analyze_filtered.RData" compare="sim_size" /> |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
541 <output name="matrixasoutput" file="analyze_matrix.tabular"/> |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
542 </test> |
1
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
543 <test expect_num_outputs="2"> |
0
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
544 <param name="infile" value="" ftype="analyze75"> |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
545 <composite_data value="Analyze75.hdr"/> |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
546 <composite_data value="Analyze75.img"/> |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
547 <composite_data value="Analyze75.t2m"/> |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
548 </param> |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
549 <conditional name="outputs"> |
1
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
550 <param name="outputs_select" value="quality_control"/> |
2
22db5eb94e50
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 1c808d60243bb1eeda0cd26cb4b0a17ab05de2c0
galaxyp
parents:
1
diff
changeset
|
551 <param name="inputmz" value="1200"/> |
1
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
552 <param name="plusminus_dalton" value="0.25"/> |
0
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
553 </conditional> |
1
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
554 <output name="filtering_qc" file="analyze75_filtered2.pdf" compare="sim_size" delta="20000"/> |
2
22db5eb94e50
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 1c808d60243bb1eeda0cd26cb4b0a17ab05de2c0
galaxyp
parents:
1
diff
changeset
|
555 <output name="msidata_filtered" file="analyze_filteredoutside.RData" compare="sim_size" /> |
0
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
556 </test> |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
557 <test expect_num_outputs="2"> |
2
22db5eb94e50
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 1c808d60243bb1eeda0cd26cb4b0a17ab05de2c0
galaxyp
parents:
1
diff
changeset
|
558 <param name="infile" value="preprocessed.RData" ftype="rdata"/> |
0
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
559 <conditional name="outputs"> |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
560 <param name="outputs_select" value="no_quality_control"/> |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
561 </conditional> |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
562 <param name="output_matrix" value="True"/> |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
563 <output name="matrixasoutput" file="rdata_matrix.tabular"/> |
1
98c101b19f3c
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 06c2b45d8644b1d7fc01622a5c59dcbf8886d0f1
galaxyp
parents:
0
diff
changeset
|
564 <output name="msidata_filtered" file="rdata_notfiltered.RData" compare="sim_size" /> |
0
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
565 </test> |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
566 </tests> |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
567 <help> |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
568 <![CDATA[ |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
569 |
2
22db5eb94e50
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 1c808d60243bb1eeda0cd26cb4b0a17ab05de2c0
galaxyp
parents:
1
diff
changeset
|
570 Cardinal is an R package that implements statistical & computational tools for analyzing mass spectrometry imaging datasets. `More information on Cardinal <http://cardinalmsi.org//>`_ |
22db5eb94e50
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 1c808d60243bb1eeda0cd26cb4b0a17ab05de2c0
galaxyp
parents:
1
diff
changeset
|
571 |
22db5eb94e50
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 1c808d60243bb1eeda0cd26cb4b0a17ab05de2c0
galaxyp
parents:
1
diff
changeset
|
572 This tool provides provides options to filter (subset) pixels and masses of mass-spectrometry imaging data. |
0
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
573 |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
574 Input data: 3 types of input data can be used: |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
575 |
2
22db5eb94e50
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 1c808d60243bb1eeda0cd26cb4b0a17ab05de2c0
galaxyp
parents:
1
diff
changeset
|
576 - imzml file (upload imzml and ibd file via the "composite" function) `Introduction to the imzml format <https://ms-imaging.org/wp/imzml/>`_ |
0
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
577 - Analyze7.5 (upload hdr, img and t2m file via the "composite" function) |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
578 - Cardinal "MSImageSet" data (with variable name "msidata", saved as .RData) |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
579 |
2
22db5eb94e50
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 1c808d60243bb1eeda0cd26cb4b0a17ab05de2c0
galaxyp
parents:
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diff
changeset
|
580 Options: |
22db5eb94e50
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 1c808d60243bb1eeda0cd26cb4b0a17ab05de2c0
galaxyp
parents:
1
diff
changeset
|
581 |
22db5eb94e50
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 1c808d60243bb1eeda0cd26cb4b0a17ab05de2c0
galaxyp
parents:
1
diff
changeset
|
582 - pixel filtering: can use a tabular file containing x and y coordinates or by defining a range for x and y by hand |
22db5eb94e50
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 1c808d60243bb1eeda0cd26cb4b0a17ab05de2c0
galaxyp
parents:
1
diff
changeset
|
583 - mass filtering: can use a tabular file containing masses of interest or by defining a range for the mass values |
22db5eb94e50
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 1c808d60243bb1eeda0cd26cb4b0a17ab05de2c0
galaxyp
parents:
1
diff
changeset
|
584 |
22db5eb94e50
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 1c808d60243bb1eeda0cd26cb4b0a17ab05de2c0
galaxyp
parents:
1
diff
changeset
|
585 Output: |
22db5eb94e50
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 1c808d60243bb1eeda0cd26cb4b0a17ab05de2c0
galaxyp
parents:
1
diff
changeset
|
586 |
22db5eb94e50
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 1c808d60243bb1eeda0cd26cb4b0a17ab05de2c0
galaxyp
parents:
1
diff
changeset
|
587 - imzML file filtered for pixels and/or masses |
22db5eb94e50
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 1c808d60243bb1eeda0cd26cb4b0a17ab05de2c0
galaxyp
parents:
1
diff
changeset
|
588 - optional: pdf with heatmap showing the pixels that are left after filtering and plot of masses before and after filtering |
22db5eb94e50
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 1c808d60243bb1eeda0cd26cb4b0a17ab05de2c0
galaxyp
parents:
1
diff
changeset
|
589 - optional: intensity matrix as tabular file (intensities for masses in rows and pixel in columns) |
22db5eb94e50
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 1c808d60243bb1eeda0cd26cb4b0a17ab05de2c0
galaxyp
parents:
1
diff
changeset
|
590 |
22db5eb94e50
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 1c808d60243bb1eeda0cd26cb4b0a17ab05de2c0
galaxyp
parents:
1
diff
changeset
|
591 Tip: |
22db5eb94e50
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 1c808d60243bb1eeda0cd26cb4b0a17ab05de2c0
galaxyp
parents:
1
diff
changeset
|
592 |
22db5eb94e50
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 1c808d60243bb1eeda0cd26cb4b0a17ab05de2c0
galaxyp
parents:
1
diff
changeset
|
593 - It is recommended to use the filtering tool only for masses which have been extracted from the same dataset. If you have masses from dataset A and you want to use them to filter dataset B, first find the corresponding (closest) features in dataset B by using the tool "Join two files on column allowing a small difference". Afterwards use the corresponding feature masses from dataset A to filter dataset B. |
22db5eb94e50
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 1c808d60243bb1eeda0cd26cb4b0a17ab05de2c0
galaxyp
parents:
1
diff
changeset
|
594 |
22db5eb94e50
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/msi_filtering commit 1c808d60243bb1eeda0cd26cb4b0a17ab05de2c0
galaxyp
parents:
1
diff
changeset
|
595 |
0
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
596 ]]> |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
597 </help> |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
598 <citations> |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
599 <citation type="doi">10.1093/bioinformatics/btv146</citation> |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
600 </citations> |
f17d3f1a065f
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/msi_filtering commit 3363c40790b0d64a085f980980f4289165eed27f
galaxyp
parents:
diff
changeset
|
601 </tool> |