view README.rst @ 0:cb2a42d4a5ac default tip

Initial upload
author Jim Johnson <jj@umn.edu>
date Mon, 17 Mar 2014 15:55:58 -0500
parents
children
line wrap: on
line source

This package contains a Galaxy workflow that determines if peptides identified from MS searches are present in the reference proteome.  
Tool also allows for peptide annotation, for each novel peptide, by retrieving accession headers for those polypeptide sequences that exactly match the novel peptide.

See http://www.galaxyproject.org for information about the Galaxy Project.


Availability
============

This workflow is available to download and/or install from the main
Galaxy Tool Shed:

http://toolshed.g2.bx.psu.edu/view/galaxyp/proteomics_novel_peptide_filter_workflow


Reference Data
==============

For Human RNAseq data this workflow was tested using reference data from:

* ftp://ftp.ensembl.org/pub/release-73/fasta/homo_sapiens/pep/Homo_sapiens.GRCh37.73.pep.all.fa.gz


For Mouse RNAseq data this workflow was tested using reference data from:

* ftp://ftp.ensembl.org/pub/release-73/fasta/mus_musculus/pep/Mus_musculus.GRCm38.73.pep.all.fa.gz


Dependencies
============

These dependencies should be resolved automatically via the Galaxy Tool Shed:

* http://toolshed.g2.bx.psu.edu/view//jjohnson/find_in_reference


History
=======

======= ======================================================================
Version Changes
------- ----------------------------------------------------------------------
v0.0.1  - Initial release to Tool Shed (March, 2014)
======= ======================================================================