annotate scimap_phenotyping.py @ 2:ce22e846c5e4 draft

planemo upload for repository https://github.com/goeckslab/tools-mti/tree/main/tools/scimap commit 9fb5578191db8a559191e45156cfb95350f01aea

import argparse
import warnings

import pandas as pd
import scimap as sm
from anndata import read_h5ad


def main(
    adata,
    output,
    log,
    gating_workflow,
    gating_workflow_ext,
    manual_gates=None,
    manual_gates_ext=None,
    random_state=0
):
    """
    Parameter
    ---------
    adata : str
        File path to the input AnnData.
    output : str
        File path to the output AnnData.
    log: bool
        Boolean whether to log the input data prior to rescaling
    gating_workflow : str
        File path to the gating workflow.
    gating_workflow_ext : str
        Datatype for gating workflow, either 'csv' or 'tabular'.
    manual_gates : str
        File path to the munual gating.
    manual_gates_ext : str
        Datatype for munual gate, either 'csv' or 'tabular'.
    random_state: int
        The seed used by the random number generator for GMM in sm.pp.rescale
    """
    warnings.simplefilter('ignore')

    adata = read_h5ad(adata)
    # Rescale data
    if manual_gates:
        sep = ',' if manual_gates_ext == 'csv' else '\t'
        manual_gates = pd.read_csv(manual_gates, sep=sep)

    adata = sm.pp.rescale(
        adata,
        gate=manual_gates,
        log=log,
        random_state=random_state
    )

    # Phenotype cells
    # Load the gating workflow
    sep = ',' if gating_workflow_ext == 'csv' else '\t'
    phenotype = pd.read_csv(gating_workflow, sep=sep)
    adata = sm.tl.phenotype_cells(
        adata,
        phenotype=phenotype,
        label="phenotype"
    )

    # Summary of the phenotyping
    print(adata.obs['phenotype'].value_counts())

    adata.write(output)


if __name__ == '__main__':
    aparser = argparse.ArgumentParser()
    aparser.add_argument("-a", "--adata", dest="adata", required=True)
    aparser.add_argument("-o", "--output", dest="output", required=True)
    aparser.add_argument("-l", "--log", dest="log", action="store_true")
    aparser.add_argument(
        "-g",
        "--gating_workflow",
        dest="gating_workflow",
        required=True
    )
    aparser.add_argument(
        "-s",
        "--gating_workflow_ext",
        dest="gating_workflow_ext",
        required=True
    )
    aparser.add_argument(
        "-m",
        "--manual_gates",
        dest="manual_gates",
        required=False
    )
    aparser.add_argument(
        "-S",
        "--manual_gates_ext",
        dest="manual_gates_ext",
        required=False
    )
    aparser.add_argument(
        "--random_state",
        dest="random_state",
        type=int,
        required=False
    )

    args = aparser.parse_args()

    if args.log:
        print("\n adata.raw.X will be log1p transformed \n")

    main(
        adata=args.adata,
        output=args.output,
        log=args.log,
        gating_workflow=args.gating_workflow,
        gating_workflow_ext=args.gating_workflow_ext,
        manual_gates=args.manual_gates,
        manual_gates_ext=args.manual_gates_ext,
        random_state=args.random_state
    )