view call_insertions.xml @ 0:5626dc2d0ef2 draft default tip

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author greg
date Tue, 07 Feb 2023 21:43:42 +0000
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<tool id="call_insertions" name="PIMA: call insertions" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" profile="@PROFILE@">
    <description></description>
    <macros>
        <import>macros.xml</import>
    </macros>
    <expand macro="requirements"/>
    <command detect_errors="exit_code"><![CDATA[
grep AvgIdentity '$dnadiff_report' | head -1 | awk '{print $2}' > reference_identity &&
grep AlignedBases '$dnadiff_report' | head -1 | awk '{sub(/\(.*/, "", $2); print $2 * 100}' > reference_aligned_bases &&
cat '$dnadiff_1coords' | awk '{OFS = "\t"; if ($2 < $1){t = $2; $2 = $1; $1 = t} print $12,$1,$2}' | sort -k 1,1 -k 2,2n > '$reference_aligned' &&
cat '$dnadiff_1coords' | awk '{OFS = "\t"; if ($4 < $3){t = $4; $4 = $3; $3 = t} print $13,$3,$4}' | sort -k 1,1 -k 2,2n > '$genome_aligned'
]]></command>
    <inputs>
        <param argument="--dnadiff_report" type="data" format="txt" label="DNAdiff report file"/>
        <param argument="--dnadiff_1coords" type="data" format="tabular" label="DNAdiff 1coords file"/>
        <param argument="--reference_identity_min" type="float" value="98.0" min="0" label="Minimum reference identity"/>
        <param argument="--reference_alignment_min" type="float" value="97.0" min="0" label="Minimum reference alignment"/>
    </inputs>
    <outputs>
        <data name="reference_aligned" format="bed" label="${tool.name} on ${on_string} (reference alignment)"/>
        <data name="genome_aligned" format="bed" label="${tool.name} on ${on_string} (genome alignment)"/>
    </outputs>
    <tests>
        <test>
            <param name="dnadiff_report" value="dnadiff_report.txt" ftype="txt"/>
            <param name="dnadiff_1coords" value="dnadiff_1coords.tsv" ftype="tsv"/>
            <output name="reference_aligned" value="reference_aligned.bed" ftype="bed"/>
            <output name="genome_aligned" value="genome_aligned.bed" ftype="bed"/>
        </test>
    </tests>
    <help>
**What it does**

Accepts DNAdiff report and DNAdiff 1coords files and extracts the aligned regions of the two genomes, producing reference alignment
and genome alignment BED files.
    </help>
    <expand macro="citations"/>
</tool>