annotate macros.xml @ 1:e8eceb3a69a7 draft

planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit a9550d87541eda8b909e6aef1ad8cbf9e9e20d06
author iuc
date Wed, 13 Jul 2016 10:57:34 -0400
parents 5add48c63b9a
children 7715aa60917a
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5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
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1
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2 <macros>
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3 <token name="@VERSION@">1.3</token>
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4 <xml name="stdio">
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5 <stdio>
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6 <exit_code range="1:" />
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7 <exit_code range=":-1" />
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8 <regex match="Error:" />
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9 <regex match="Exception:" />
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10 </stdio>
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11 </xml>
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12 <xml name="requirements">
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13 <requirements>
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14 <requirement type="package" version="1.3">bcftools</requirement>
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15 <!-- conda dependency -->
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16 <requirement type="package" version="1.3">htslib</requirement>
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17 <requirement type="package" version="0.2.6">tabix</requirement>
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18 <requirement type="package" version="1.2">samtools</requirement>
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19 </requirements>
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20 </xml>
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21 <xml name="version_command">
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22 <version_command>bcftools 2&gt;&amp;1 | grep 'Version:'</version_command>
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23 </xml>
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24
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25 <xml name="citations">
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26 <citations>
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27 <citation type="doi">10.1093/bioinformatics/btp352</citation>
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28 <yield />
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29 </citations>
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30 </xml>
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31 <token name="@BCFTOOLS_WIKI@">https://github.com/samtools/bcftools/wiki</token>
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32 <token name="@BCFTOOLS_MANPAGE@">http://samtools.github.io/bcftools/bcftools.html</token>
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33 <token name="@THREADS@">
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34 --threads \${GALAXY_SLOTS:-4}
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35 </token>
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36 <token name="@PREPARE_ENV@">
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37 <![CDATA[
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38 export BCFTOOLS_PLUGINS=`which bcftools | sed 's,bin/bcftools,libexec/bcftools,'`;
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39 ]]>
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40 </token>
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41 <xml name="macro_input">
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42 <param name="input_file" type="data" format="vcf,vcf_bgzip,bcf,bcf_bgzip" label="VCF/BCF Data" />
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43 </xml>
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44 <token name="@PREPARE_INPUT_FILE@">
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45 <![CDATA[
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46 ## May need to symlink input if there is an associated
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47 #set $input_vcf = 'input.vcf.gz'
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48 #if $input_file.datatype.file_ext == 'vcf'
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49 bgzip -c "$input_file" > $input_vcf &&
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50 bcftools index $input_vcf &&
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51 #elif $input_file.datatype.file_ext == 'vcf_bgzip'
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52 ln -s "$input_file" $input_vcf
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53 #elif $input_file.datatype.file_ext == 'bcf'
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54 #set $input_vcf = 'input.bcf'
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55 ln -s "$input_file" $input_vcf &&
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56 #if $input_file.metadata.bcf_index:
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57 ln -s $input_file.metadata.bcf_index ${input_vcf}.csi &&
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58 #else
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59 bcftools index $input_vcf &&
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60 #end if
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61 #elif $input_file.datatype.file_ext == 'bcf_bgzip'
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62 ln -s "$input_file" $input_vcf
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63 #end if
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64 ]]>
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65 </token>
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66 <token name="@INPUT_FILE@">
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67 $input_vcf
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68 </token>
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69
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70 <xml name="macro_inputs">
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71 <param name="input_files" type="data" format="vcf,bcf" label="Other VCF/BCF Datasets" multiple="True" />
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72 </xml>
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73 <token name="@PREPARE_INPUT_FILES@">
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74 <![CDATA[
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75 ## May need to symlink input if there is an associated
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76 #set $input_vcfs = []
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77 #set $vcfs_list_file = 'vcfs_list'
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78 #for (i,input_file) in enumerate($input_files):
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79 #set $input_vcf = 'input' + str($i) + '.vcf.gz'
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80 #if $input_file.datatype.file_ext == 'vcf'
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81 bgzip -c "$input_file" > $input_vcf &&
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82 bcftools index $input_vcf &&
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83 #elif $input_file.datatype.file_ext == 'vcf_bgz'
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84 ln -s "$input_file" $input_vcf
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85 #elif $input_file.datatype.file_ext == 'bcf'
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86 #set $input_vcf = 'input' + str($i) + '.bcf.gz'
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87 ## bgzip -c "$input_file" > $input_vcf &&
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88 ln -s "$input_file" $input_vcf &&
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89 #if $input_file.metadata.bcf_index:
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90 ln -s $input_file.metadata.bcf_index ${input_vcf}.csi &&
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91 #else
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92 bcftools index $input_vcf &&
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93 #end if
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94 #elif $input_file.datatype.file_ext == 'bcfvcf_bgz'
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95 ln -s "$input_file" $input_vcf &&
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96 #end if
1
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97 echo '$input_vcf' >> $vcfs_list_file &&
0
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98 $input_vcfs.append($input_vcf)
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99 #end for
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100 ]]>
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101 </token>
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102 <token name="@INPUT_FILES@">
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103 #echo ' '.join($input_vcfs)#
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104 </token>
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105 <token name="@INPUT_LIST_FILE@">
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106 $vcfs_list_file
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107 </token>
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108
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109 <xml name="macro_fasta_ref">
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110 <param name="fasta_ref" type="data" format="data" label="Fasta Ref" optional="True" help="reference sequence in fasta format" />
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111 </xml>
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112 <token name="@PREPARE_FASTA_REF@">
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113 <![CDATA[
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114 #set $input_fa_ref = None
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115 #if 'fasta_ref' in $section and $section.fasta_ref:
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116 #set $input_fa_ref = 'ref.fa'
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117 ln -s $section.fasta_ref $input_fa_ref &&
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118 samtools faidx $input_fa_ref &&
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119 #end if
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120 ]]>
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121 </token>
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122 <token name="@FASTA_REF@">
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123 #if $input_fa_ref is not None:
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124 --fasta-ref "$input_fa_ref"
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125 #elif 'fasta_ref' in $section and $section.fasta_ref:
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126 --fasta-ref "${section.fasta_ref}"
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127 #end if
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128 </token>
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129
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130 <xml name="macro_ref_fasta">
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131 <conditional name="reference_source">
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132 <param name="reference_source_selector" type="select" label="Choose the source for the reference genome">
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133 <option value="cached">Locally cached</option>
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134 <option value="history">History</option>
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135 </param>
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136 <when value="cached">
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137 <param name="ref_file" type="select" label="Select reference genome">
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138 <options from_data_table="fasta_indexes">
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139 <!--<filter type="data_meta" key="dbkey" ref="input_bam" column="value"/>-->
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140 </options>
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141 <validator type="no_options" message="A built-in reference genome is not available for the build associated with the selected input file"/>
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142 </param>
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143 </when>
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144 <when value="history"> <!-- FIX ME!!!! -->
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145 <param name="ref_file" type="data" format="fasta" label="Using reference file" />
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146 </when>
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147 </conditional>
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148 </xml>
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149
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150
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151 <xml name="macro_AF_file">
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152 <param name="AF_file" type="data" format="data" label="Af File" optional="True" help="read allele frequencies from file (CHR\tPOS\tREF,ALT\tAF)" />
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153 </xml>
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154 <!-- This may need to bgzip and tabix the file -->
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155 <token name="@PREPARE_AF_FILE@">
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156 <![CDATA[
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157 #if 'AF_file' in $section and $section.AF_file:
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158 #pass
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159 #end if
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160 ]]>
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161 </token>
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162 <token name="@AF_FILE@">
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163 #if 'AF_file' in $section and $section.AF_file:
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164 --AF-file "${section.AF_file}"
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165 #end if
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166 </token>
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167
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168 <xml name="macro_estimate_AF">
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169 <param name="estimate_AF" type="data" format="data" label="Estimate Af" optional="True" help="calculate AC,AN counts on the fly, using either all samples (&quot;-&quot;) or samples listed in &lt;file&gt;" />
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170 </xml>
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171 <token name="@ESTIMATE_AF@">
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172 #if 'estimate_AF' in $section and $section.estimate_AF:
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173 --estimate-AF "${section.estimate_AF}"
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174 #end if
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175 </token>
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176
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177 <xml name="macro_exons_file">
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178 <param name="exons_file" type="data" format="tabular" label="exons file" optional="True" help="tab-delimited file with exons for indel frameshifts (chr,from,to; 1-based, inclusive, bgzip compressed)" />
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179 </xml>
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180 <token name="@PREPARE_EXONS_FILE@">
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181 <![CDATA[
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182 #set $exons_path = None
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183 #if 'exons_file' in $section and $section.exons_file:
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184 #set $exons_path = 'exons_file.tab.gz'
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185 bgzip -c "$section.exons_file" > $exons_path &&
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186 tabix -s 1 -b 2 -e 3 $exons_path &&
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187 #end if
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188 ]]>
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189 </token>
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190 <token name="@EXONS_FILE@">
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191 #if 'exons_file' in $section and $section.exons_file:
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192 --exons $exons_path
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193 #end if
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194 </token>
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195
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196 <xml name="macro_ploidy_file">
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197 <param name="ploidy_file" type="data" format="tabular" label="Ploidy file" optional="True" help="tab-delimited list of CHROM,FROM,TO,SEX,PLOIDY" />
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198 </xml>
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199 <token name="@PLOIDY_FILE@">
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200 #if 'ploidy_file' in $section and $section.ploidy_file:
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201 --ploidy "${section.ploidy_file}"
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202 #end if
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203 </token>
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204
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205 <xml name="macro_collapse_opt_none">
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206 <option value="none">none - require the exact same set of alleles in all files</option>
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207 </xml>
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208 <xml name="macro_collapse_opt_id">
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209 <option value="id">id - only records with identical ID column are compatible. </option>
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210 </xml>
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211 <xml name="macro_collapse">
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212 <param name="collapse" type="select" label="Collapse" optional="True" help="Controls how to treat records with duplicate positions and defines compatible records across multiple input files">
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213 <option value="snps">snps - allow different alleles, as long as they all are SNPs</option>
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214 <option value="indels">indels - allow different alleles, as long as they all are indels</option>
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215 <option value="both">both - indels and snps </option>
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216 <option value="some">some - at least some of the ALTs must match</option>
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217 <option value="any">any - any combination of alleles</option>
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218 <yield/>
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219 </param>
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220 </xml>
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221 <token name="@COLLAPSE@">
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222 #if $section.collapse:
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223 --collapse "${section.collapse}"
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224 #end if
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225 </token>
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226
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227 <xml name="macro_apply_filters">
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228 <param name="apply_filters" type="text" value="" label="Apply Filters" optional="true"
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229 help="(-f --apply-filters) Skip sites where FILTER column does not contain any of the strings listed (e.g. &quot;PASS,.&quot;)">
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230 <validator type="regex" message="FILTER terms separated by commas">^([^ \t\n\r\f\v,]+(,[^ \t\n\r\f\v,]+)*)?$</validator>
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231 </param>
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232 </xml>
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233 <token name="@APPLY_FILTERS@">
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234 #if $section.apply_filters:
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235 --apply-filters "${section.apply_filters}"
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236 #end if
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237 </token>
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238
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239 <xml name="macro_select_output_type">
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240 <param name="output_type" type="select">
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241 <option value="b">compressed BCF</option>
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242 <!-- no galaxy datatypes for these
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243 <option value="u">uncompressed BCF</option>
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244 <option value="z">compressed VCF</option>
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245 -->
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246 <option value="v">uncompressed VCF</option>
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247 </param>
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248 </xml>
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249 <token name="@OUTPUT_TYPE@">
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250 #if str($output_type) != "__none__":
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251 --output-type "${output_type}"
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252 #end if
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253 </token>
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254
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255 <xml name="macro_vcf_output">
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256 <data name="output_file" format="vcf">
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257 <change_format>
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258 <when input="output_type" value="b" format="bcf" />
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259 <when input="output_type" value="u" format="bcf" />
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260 <when input="output_type" value="z" format="vcf_bgzip" />
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261 <when input="output_type" value="v" format="vcf" />
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262 </change_format>
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263 </data>
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264 </xml>
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265
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266 <xml name="macro_regions">
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267 <conditional name="regions">
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268 <param name="regions_src" type="select" label="Regions">
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269 <option value="__none__">None</option>
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270 <option value="regions">regions</option>
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271 <option value="regions_file">regions-file</option>
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272 </param>
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273 <when value="__none__"/>
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274 <when value="regions">
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275 <param name="regions" type="text" value="" label="restrict to comma-separated list of regions" optional="true"
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276 help="Each region is specifed as: chr or chr:pos or chr:from-to">
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277 <validator type="regex" message="">^(\w+(:\d+(-\d+)?)?(,\w+(:\d+(-\d+)?)?)*)?$</validator>
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278 </param>
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279 </when>
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280 <when value="regions_file">
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281 <param name="regions_file" type="data" format="vcf,bed,tabular" label="Regions File" optional="True" help="restrict to regions listed in a file" />
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282 </when>
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283 </conditional>
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284 </xml>
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285 <token name="@REGIONS@">
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286 #if $section.regions.regions_src == 'regions' and $section.regions.regions != '':
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287 --regions "$section.regions.regions"
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288 #elif $section.regions.regions_src == 'regions_file' and $section.regions.regions_file:
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289 --regions-file "$section.regions.regions_file"
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290 #end if
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291 </token>
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292
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293 <xml name="macro_targets_file">
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294 <param name="targets_file" type="data" format="tabular" label="Targets File" help="restrict to targets listed in a file" >
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295 <yield/>
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296 </param>
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297 <param name="invert_targets_file" type="boolean" truevalue="^" falsevalue="" label="Invert Targets" help="inverts the query/filtering applied by the target file selection" />
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298 </xml>
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299 <token name="@PREPARE_TARGETS_FILE@">
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300 <![CDATA[
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301 #set $targets_path = None
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302 #if 'targets' in $section
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303 #if $section.targets.targets_src == 'targets_file':
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304 #set $targets_path = 'targets_file.tab.gz'
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305 bgzip -c "$section.targets.targets_file" > $targets_path &&
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306 tabix -s 1 -b 2 -e 2 $targets_path &&
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307 #end if
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308 #elif $tgts_sec.targets_file:
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309 #set $targets_path = 'targets_file.tab.gz'
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310 bgzip -c "$section.targets_file" > $targets_path &&
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311 tabix -s 1 -b 2 -e 2 $targets_path &&
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312 #end if
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313 ]]>
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314 </token>
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315 <token name="@TARGETS_FILE@">
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316 <![CDATA[
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317 #if $targets_path is not None:
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318 --targets-file "${section.invert_targets_file}${targets_path}"
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319 #elif $section.targets_file:
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320 --targets-file "${section.invert_targets_file}${section.targets_file}"
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321 #end if
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322 ]]>
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323 </token>
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324
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325 <xml name="macro_targets">
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326 <conditional name="targets">
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327 <param name="targets_src" type="select" label="Targets">
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328 <option value="__none__">None</option>
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329 <option value="targets">targets</option>
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330 <option value="targets_file">targets-file</option>
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331 </param>
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332 <when value="__none__"/>
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333 <when value="targets">
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334 <param name="targets" type="text" value="" label="Restrict to comma-separated list of targets" optional="true"
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335 help="Each target is specifed as: chr or chr:pos or chr:from-to">
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336 <validator type="regex" message="">^(\w+(:\d+(-\d+)?)?(,\w+(:\d+(-\d+)?)?)*)?$</validator>
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337 </param>
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338 <param name="invert_targets_file" type="boolean" truevalue="^" falsevalue="" label="Invert Targets" help="inverts the query/filtering applied by the targets" />
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339 </when>
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340 <when value="targets_file">
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341 <expand macro="macro_targets_file">
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342 <optional>true</optional>
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343 </expand>
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344 </when>
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345 </conditional>
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346 </xml>
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347 <token name="@TARGETS@">
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348 <![CDATA[
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349 #if $targets_path:
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350 --targets-file "${section.targets.invert_targets_file}${targets_path}"
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351 #else:
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352 #if $section.targets.targets_src == 'targets' and $section.targets.targets != '':
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353 --targets "${section.targets.invert_targets_file}${section.targets.targets}"
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354 #elif $section.targets.targets_src == 'targets_file' and $section.targets.targets_file:
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355 --targets-file "${section.targets.invert_targets_file}${section.targets.targets_file}"
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356 #end if
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357 #end if
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358 ]]>
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359 </token>
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360
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361 <xml name="macro_samples">
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362 <param name="samples" type="text" value="" label="Samples" optional="true"
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363 help="(-s) comma separated list of samples to annotate (or exclude with &quot;^&quot; prefix)">
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364 <validator type="regex" message="">^(\w+(,\w+)*)?$</validator>
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365 </param>
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366 <param name="invert_samples" type="boolean" truevalue="^" falsevalue="" checked="false" label="Invert Samples"
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diff changeset
367 help="inverts the query/filtering applied by Samples" />
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
368 <param name="samples_file" type="data" format="tabular" label="Samples File" optional="True"
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
369 help="(-S) file of samples to include" />
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
370 <param name="invert_samples_file" type="boolean" truevalue="^" falsevalue="" checked="false" label="Invert Samples File"
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
371 help="inverts the query/filtering applied by Samples File" />
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
372 </xml>
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
373 <token name="@SAMPLES@">
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
374 #set $samples_defined = False
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
375 #if str($section.samples) != '':
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
376 #set $samples_defined = True
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
377 --samples "${section.invert_samples}${section.samples}"
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
378 #end if
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
379 #if $section.samples_file:
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
380 #set $samples_defined = True
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
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diff changeset
381 --samples-file "${section.invert_samples_file}${section.samples_file}"
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
382 #end if
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
383 </token>
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
384
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
385 <xml name="macro_sample">
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
386 <param name="sample" type="text" label="Sample" optional="True" help="apply variants of the given sample" />
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
387 </xml>
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
388 <token name="@SAMPLE@">
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
389 #if $section.sample:
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
390 --sample "${section.sample}"
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
391 #end if
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
392 </token>
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
393
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
394
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
395 <xml name="macro_include">
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
396 <param name="include" type="text" label="Include" optional="True" help="(-i) select sites for which the expression is true">
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
397 <validator type="regex" message="Single quote not allowed">^[^']*$</validator>
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
398 <sanitizer sanitize="False"/>
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
399 </param>
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
400 </xml>
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
401 <token name="@INCLUDE@">
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
402 #if $section.include:
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
403 --include '${section.include}'
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
404 #end if
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
405 </token>
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
406
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
407 <xml name="macro_exclude">
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
408 <param name="exclude" type="text" label="Exclude" optional="True" help="(-e) exclude sites for which the expression is true">
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
409 <validator type="regex" message="Single quote not allowed">^[^']*$</validator>
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
410 <sanitizer sanitize="False"/>
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
411 </param>
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
412 </xml>
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
413 <token name="@EXCLUDE@">
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
414 #if $section.exclude:
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
415 --exclude '${section.exclude}'
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
416 #end if
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
417 </token>
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
418
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
419 <xml name="macro_columns">
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
420 <param name="columns" type="text" value="" label="Columns" optional="true"
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
421 help="list of columns in the annotation file, e.g. CHROM,POS,REF,ALT,-,INFO/TAG. See man page for details">
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
422 <validator type="regex" message="COLUMN names separated by commas">^([^,]+(,[^,]+)*)?$</validator>
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
423 </param>
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
424 </xml>
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
425 <token name="@COLUMNS@">
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
426 #if $section.columns != '':
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
427 --columns "${section.columns}"
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
428 #end if
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
429 </token>
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
430
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
431 <xml name="macro_haploid2diploid">
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
432 <param name="haploid2diploid" type="boolean" truevalue="--haploid2diploid" falsevalue="" label="Haploid2Diploid" help="convert haploid genotypes to diploid homozygotes" />
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
433 </xml>
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
434
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
435 <xml name="macro_vcf_ids">
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
436 <param name="vcf_ids" type="boolean" truevalue="--vcf-ids" falsevalue="" label="Vcf Ids" help="output VCF IDs instead of CHROM:POS_REF_ALT" />
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
437 </xml>
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
438 <token name="@VCF_IDS@">
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
439 ${section.vcf_ids}
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
440 </token>
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
441
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
442 <token name="@OUTPUT_HELP@">
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
443 <![CDATA[
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
444 Output Type
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
445 -----------
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
446
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
447 Output compressed BCF (b), or uncompressed VCF (v).
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
448 Use the BCF option when piping between bcftools subcommands to speed up
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
449 performance by removing unecessary compression/decompression
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
450 and VCF<->BCF conversion.
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
451
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
452 This Galaxy tool recommends using the compressed BCF format
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
453 as piping is not implemented, and uncompressed data would
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
454 use unnecessary amounts of space.
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
455
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
456 ]]></token>
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
457 <token name="@REGIONS_HELP@">
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
458 <![CDATA[
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
459 Region Selections
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
460 -----------------
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
461
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
462 Regions can be specified in a VCF,
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
463 BED, or tab-delimited file (the default). The columns of the
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
464 tab-delimited file are: CHROM, POS, and, optionally, POS_TO,
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
465 where positions are 1-based and inclusive. Uncompressed
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
466 files are stored in memory, while bgzip-compressed and
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
467 tabix-indexed region files are streamed. Note that sequence
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
468 names must match exactly, "chr20" is not the same as "20".
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
469 Also note that chromosome ordering in FILE will be
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
470 respected, the VCF will be processed in the order in which
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
471 chromosomes first appear in FILE. However, within
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
472 chromosomes, the VCF will always be processed in ascending
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
473 genomic coordinate order no matter what order they appear in
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
474 FILE. Note that overlapping regions in FILE can result in
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
475 duplicated out of order positions in the output. This option
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
476 requires indexed VCF/BCF files.
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
477
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
478 ]]></token>
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
479 <token name="@TARGETS_HELP@"><![CDATA[
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
480 Targets
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
481 -------
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
482
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
483 Similar to regions, but the next position is accessed by streaming the whole
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
484 VCF/BCF rather than using the tbi/csi index. Both regions and targets options can be
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
485 applied simultaneously: regions uses the index to jump to a region and targets discards
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
486 positions which are not in the targets. Unlike regions, targets can be prefixed with
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
487 "^" to request logical complement. For example, "^X,Y,MT" indicates that
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
488 sequences X, Y and MT should be skipped. Yet another difference between the two
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
489 is that regions checks both start and end positions of indels, whereas targets checks
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
490 start positions only.
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
491
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
492 For the bcftools call command, with the option -C alleles, third column of the
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
493 targets file must be comma-separated list of alleles, starting with the
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
494 reference allele. Note that the file must be compressed and index. Such a file
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
495 can be easily created from a VCF using::
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
496
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
497 bcftools query -f'%CHROM\t%POS\t%REF,%ALT\n' file.vcf | bgzip -c > als.tsv.gz && tabix -s1 -b2 -e2 als.tsv.gz
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
498 ]]>
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
499 <!-- TODO: galaxy-ify -->
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
500 </token>
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
501
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
502
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
503 <token name="@COLLAPSE_HELP@">
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
504 Collapse
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
505 --------
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
506
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
507 Controls how to treat records with duplicate positions and defines compatible
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
508 records across multiple input files. Here by "compatible" we mean records which
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
509 should be considered as identical by the tools. For example, when performing
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
510 line intersections, the desire may be to consider as identical all sites with
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
511 matching positions (bcftools isec -c all), or only sites with matching variant
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
512 type (bcftools isec -c snps -c indels), or only sites with all alleles
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
513 identical (bcftools isec -c none).
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
514
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
515
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
516 +------------+----------------------------------------------------------------+
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
517 | Flag value | Result |
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
518 +============+================================================================+
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
519 | none | only records with identical REF and ALT alleles are compatible |
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
520 +------------+----------------------------------------------------------------+
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
521 | some | only records where some subset of ALT alleles match are |
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
522 | | compatible |
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
523 +------------+----------------------------------------------------------------+
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
524 | all | all records are compatible, regardless of whether the ALT |
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
525 | | alleles match or not. In the case of records with the same |
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
526 | | position, only the first wil lbe considered and appear on |
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
527 | | output. |
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
528 +------------+----------------------------------------------------------------+
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
529 | snps | any SNP records are compatible, regardless of whether the ALT |
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
530 | | alleles match or not. For duplicate positions, only the first |
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
531 | | SNP record will be considered and appear on output. |
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
532 +------------+----------------------------------------------------------------+
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
533 | indels | all indel records are compatible, regardless of whether the |
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
534 | | REF and ALT alleles match or not. For duplicate positions, |
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
535 | | only the first indel record will be considered and appear on |
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
536 | | output. |
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
537 +------------+----------------------------------------------------------------+
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
538 | both | abbreviation of "-c indels -c snps" |
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
539 +------------+----------------------------------------------------------------+
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
540 | id | only records with identical ID column are compatible. |
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
541 | | Supportedby bcftools merge only. |
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
542 +------------+----------------------------------------------------------------+
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
543
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
544 </token>
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
545
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
546 <token name="@EXPRESSIONS_HELP@">
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
547 <![CDATA[
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
548 Expressions
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
549 -----------
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
550
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
551 Valid expressions may contain:
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
552
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
553 - numerical constants, string constants
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
554
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
555 ::
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
556
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
557 1, 1.0, 1e-4
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
558 "String"
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
559
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
560 - arithmetic operators
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
561
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
562 ::
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
563
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
564 +,*,-,/
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
565
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
566 - comparison operators
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
567
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
568 ::
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
569
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
570 == (same as =), >, >=, <=, <, !=
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
571
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
572 - regex operators "~" and its negation "!~"
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
573
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
574 ::
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
575
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
576 INFO/HAYSTACK ~ "needle"
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
577
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
578 - parentheses
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
579
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
580 ::
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
581
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
582 (, )
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
583
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
584 - logical operators
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
585
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
586 ::
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
587
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
588 && (same as &), ||, |
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
589
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
590 - INFO tags, FORMAT tags, column names
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
591
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
592 ::
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
593
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
594 INFO/DP or DP
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
595 FORMAT/DV, FMT/DV, or DV
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
596 FILTER, QUAL, ID, REF, ALT[0]
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
597
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
598 - 1 (or 0) to test the presence (or absence) of a flag
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
599
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
600 ::
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
601
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
602 FlagA=1 && FlagB=0
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
603
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
604 - "." to test missing values
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
605
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
606 ::
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
607
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
608 DP=".", DP!=".", ALT="."
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
609
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
610 - missing genotypes can be matched regardless of phase and ploidy (".|.", "./.", ".") using this expression
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
611
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
612 ::
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
613
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
614 GT="."
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
615
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
616 - TYPE for variant type in REF,ALT columns (indel,snp,mnp,ref,other)
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
617
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
618 ::
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
619
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
620 TYPE="indel" | TYPE="snp"
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
621
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
622 - array subscripts, "*" for any field
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
623
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
624 ::
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
625
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
626 (DP4[0]+DP4[1])/(DP4[2]+DP4[3]) > 0.3
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
627 DP4[*] == 0
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
628 CSQ[*] ~ "missense_variant.*deleterious"
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
629
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
630 - function on FORMAT tags (over samples) and INFO tags (over vector fields)
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
631
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
632 ::
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
633
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
634 MAX, MIN, AVG, SUM, STRLEN, ABS
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
635
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
636 - variables calculated on the fly if not present: number of alternate alleles; number of samples; count of alternate alleles; minor allele count (similar to AC but is always smaller than 0.5); frequency of alternate alleles (AF=AC/AN); frequency of minor alleles (MAF=MAC/AN); number of alleles in called genotypes
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
637
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
638 ::
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
639
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
640 N_ALT, N_SAMPLES, AC, MAC, AF, MAF, AN
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
641
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
642 **Notes:**
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
643
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
644 - String comparisons and regular expressions are case-insensitive
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
645 - If the subscript "*" is used in regular expression search, the whole field
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
646 is treated as one string. For example, the regex ``STR[*]~"B,C"`` will be
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
647 true for the string vector INFO/STR=AB,CD.
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
648 - Variables and function names are case-insensitive, but not tag names. For
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
649 example, "qual" can be used instead of "QUAL", "strlen()" instead of
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
650 "STRLEN()" , but not "dp" instead of "DP".
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
651
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
652 **Examples:**
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
653
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
654 ::
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
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655
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
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656 MIN(DV)>5
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
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657 MIN(DV/DP)>0.3
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
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658 MIN(DP)>10 & MIN(DV)>3
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
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659 FMT/DP>10 & FMT/GQ>10 .. both conditions must be satisfied within one sample
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
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660 FMT/DP>10 && FMT/GQ>10 .. the conditions can be satisfied in different samples
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
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661 QUAL>10 | FMT/GQ>10 .. selects only GQ>10 samples
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
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662 QUAL>10 || FMT/GQ>10 .. selects all samples at QUAL>10 sites
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
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663 TYPE="snp" && QUAL>=10 && (DP4[2]+DP4[3] > 2)
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
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664 MIN(DP)>35 && AVG(GQ)>50
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
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665 ID=@file .. selects lines with ID present in the file
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
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666 ID!=@~/file .. skip lines with ID present in the ~/file
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
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667 MAF[0]<0.05 .. select rare variants at 5% cutoff
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
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668
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
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669 ]]></token>
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
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670
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
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671
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
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672
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
iuc
parents:
diff changeset
673
5add48c63b9a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit ef90c4602bdb83ea7455946c9d175ea27284e643
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674 </macros>