Mercurial > repos > iuc > chira_merge
changeset 0:5db894671434 draft
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chira commit e4f841daf49048d6c656d50cffb344b53eebeec2"
| author | iuc |
|---|---|
| date | Sun, 19 Jan 2020 16:31:34 -0500 |
| parents | |
| children | ce26b5a859ba |
| files | all_fasta.loc.sample chira_merge.xml macros.xml test-data/alignments.bed test-data/annotation.gtf test-data/chimeras test-data/loci.counts test-data/merged.bed test-data/reads.fasta test-data/reads.fastq test-data/ref.fasta test-data/ref1.fasta test-data/ref2.fasta test-data/segments.bed test-data/singletons tool_data_table_conf.xml.sample |
| diffstat | 16 files changed, 715 insertions(+), 0 deletions(-) [+] |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/all_fasta.loc.sample Sun Jan 19 16:31:34 2020 -0500 @@ -0,0 +1,18 @@ +#This file lists the locations and dbkeys of all the fasta files +#under the "genome" directory (a directory that contains a directory +#for each build). The script extract_fasta.py will generate the file +#all_fasta.loc. This file has the format (white space characters are +#TAB characters): +# +#<unique_build_id> <dbkey> <display_name> <file_path> +# +#So, all_fasta.loc could look something like this: +# +#apiMel3 apiMel3 Honeybee (Apis mellifera): apiMel3 /path/to/genome/apiMel3/apiMel3.fa +#hg19canon hg19 Human (Homo sapiens): hg19 Canonical /path/to/genome/hg19/hg19canon.fa +#hg19full hg19 Human (Homo sapiens): hg19 Full /path/to/genome/hg19/hg19full.fa +# +#Your all_fasta.loc file should contain an entry for each individual +#fasta file. So there will be multiple fasta files for each build, +#such as with hg19 above. +#
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/chira_merge.xml Sun Jan 19 16:31:34 2020 -0500 @@ -0,0 +1,97 @@ +<tool id="chira_merge" name="ChiRA merge" version="@WRAPPER_VERSION@0"> + <description>merge aligned positions</description> + <macros> + <import>macros.xml</import> + </macros> + <expand macro="requirements" /> + <command><![CDATA[ + chira_merge.py + -b '$alignments' + #if str($annotation.choice) == "yes": + -g '$annotation.gtf' + #end if + -ao '$alignment_overlap' + -so '$segment_overlap' + -o ./ + ]]></command> + + <inputs> + <param format="bed" name="alignments" type="data" label="Input BED file of alignments"/> + <conditional name="annotation"> + <param name="choice" type="select" label="Hybridize interacting loci?" + help="Hybridization is a time consuming process."> + <option value="yes">Yes</option> + <option value="no">No</option> + </param> + <when value="yes"> + <param format="gtf,gff" name="gtf" type="data" label="Annotations in GTF format"/> + </when> + <when value="no"> + <!-- Do nothing --> + </when> + </conditional> + <param name="alignment_overlap" type="float" value="0.7" label="Overlap fraction for merging alignments" min="0" max="1" + help="Minimum fraction of BED entries that must overlap inorder to merge"/> + <param name="segment_overlap" type="float" value="0.7" label="Overlap fraction for merging mapped read positions to segments" min="0" max="1" + help="Matching read positions with greater than this fraction overlap are merged into a segment"/> + </inputs> + <outputs> + <data format="bed" name="segments_bed" from_work_dir="segments.bed" label="ChiRA aligned read segments on ${on_string}"/> + <data format="tabular" name="merged_bed" from_work_dir="merged.bed" label="ChiRA merged alignments on ${on_string}"/> + </outputs> + + <tests> + <!-- Test: Map without annotation --> + <test expect_num_outputs="2"> + <param name="alignments" value="alignments.bed"/> + <param name="choice" value="no" /> + <output name="segments_bed" > + <assert_contents> + <has_text_matching expression="ENSMUST00000160533\t69\t82\t6|1|4,ENSMUST00000160533,69,82,+,42S13M\t0\t+" /> + </assert_contents> + </output> + <output name="merged_bed" > + <assert_contents> + <has_text_matching expression="ENSMUST00000182010\t19\t74\t+\t6|1|1,ENSMUST00000182010,19,68,+,5S49M1S;7|9|1,ENSMUST00000182010,24,74,+,5S50M" /> + </assert_contents> + </output> + </test> + <!-- Test: Map with annotation --> + <test expect_num_outputs="2"> + <param name="alignments" value="alignments.bed" /> + <param name="choice" value="yes" /> + <param name="gtf" value="annotation.gtf" /> + <output name="segments_bed" > + <assert_contents> + <has_text_matching expression="5\t137142331\t137142344\t6|1|4,ENSMUST00000160533,69,82,+,42S13M\t0\t-" /> + </assert_contents> + </output> + <output name="merged_bed" > + <assert_contents> + <has_text_matching expression="17\t39846976\t39847031\t+\t6|1|1,ENSMUST00000182010,19,68,+,5S49M1S;7|9|1,ENSMUST00000182010,24,74,+,5S50M" /> + </assert_contents> + </output> + </test> + </tests> + + <help> + +.. class:: infomark + +**What it does** + +This tool merges the overlapping aligned positions to define the read concentrated loci. If an annotation GTF file produced, the transcriptomic alignment positions are first converted to their corresponding genomic positions. + +**Inputs** + +* Alignments in BED format +* An annotation GTF file contaning reference genomic positions. + +**Output** + +* BED file containing the alignments with reads categorized into segments depending on which part of the read is aligned. +* Tabular file containing merged alignments. 4th column contains all the alignments merged into that location. + + </help> + <expand macro="citations" /> +</tool>
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/macros.xml Sun Jan 19 16:31:34 2020 -0500 @@ -0,0 +1,15 @@ +<macros> + <token name="@WRAPPER_VERSION@">@TOOL_VERSION@+galaxy</token> + <token name="@TOOL_VERSION@">1.0.2</token> + <xml name="requirements"> + <requirements> + <requirement type="package" version="@TOOL_VERSION@">chira</requirement> + <yield/> + </requirements> + </xml> + <xml name="citations"> + <citations> + <yield /> + </citations> + </xml> +</macros>
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35|9,ENSMUST00000182010,424,445,+,5S21M29S 0 + +ENSMUST00000182010 426 445 15|2,ENSMUST00000182010,426,445,+,5S19M31S 0 + +ENSMUST00000182010 428 445 36|10,ENSMUST00000182010,428,445,+,5S17M33S 0 + +ENSMUST00000182010 430 445 30|4,ENSMUST00000182010,430,445,+,5S15M35S 0 + +ENSMUST00000182010 446 458 22|1,ENSMUST00000182010,446,458,+,43S12M 0 + +ENSMUST00000182010 446 459 12|3,ENSMUST00000182010,446,459,+,42S13M 0 + +ENSMUST00000182010 446 458 34|1,ENSMUST00000182010,446,458,+,32S12M11S 0 + +ENSMUST00000182010 446 468 35|9,ENSMUST00000182010,446,468,+,27S22M6S 0 + +ENSMUST00000182010 446 468 15|2,ENSMUST00000182010,446,468,+,25S22M8S 0 + +ENSMUST00000182010 446 468 36|10,ENSMUST00000182010,446,468,+,23S22M10S 0 + +ENSMUST00000182010 446 468 30|4,ENSMUST00000182010,446,468,+,21S22M12S 0 + +ENSMUST00000064314 967 982 23|1,ENSMUST00000064314,967,982,+,18S15M22S 0 +
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/annotation.gtf Sun Jan 19 16:31:34 2020 -0500 @@ -0,0 +1,58 @@ +14 . miRNA 115044183 115044205 . + . ID=MIMAT0000529;Alias=MIMAT0000529;Name=mmu-miR-20a-5p;Derives_from=MI0000568 +16 . miRNA 93369775 93369796 . + . ID=MIMAT0017240;Alias=MIMAT0017240;Name=mmu-miR-802-3p;Derives_from=MI0004249 +1 . miRNA 36348732 36348753 . - . ID=MIMAT0027696;Alias=MIMAT0027696;Name=mmu-miR-6898-5p;Derives_from=MI0022745 +18 . miRNA 37854605 37854625 . - . ID=MIMAT0027861;Alias=MIMAT0027861;Name=mmu-miR-6979-3p;Derives_from=MI0022827 +11 havana transcript 116338339 116347444 . - . gene_id "ENSMUSG00000052949"; gene_version "10"; transcript_id "ENSMUST00000137264"; transcript_version "1"; gene_name "Rnf157"; gene_source "ensembl_havana"; gene_biotype "protein_coding"; transcript_name "Rnf157-011"; transcript_source "havana"; transcript_biotype "processed_transcript"; transcript_support_level "3"; +11 havana exon 116347342 116347444 . - . gene_id "ENSMUSG00000052949"; gene_version "10"; transcript_id "ENSMUST00000137264"; transcript_version "1"; exon_number "1"; gene_name "Rnf157"; gene_source "ensembl_havana"; gene_biotype "protein_coding"; transcript_name "Rnf157-011"; transcript_source "havana"; transcript_biotype "processed_transcript"; exon_id "ENSMUSE00000834936"; exon_version "1"; transcript_support_level "3"; +11 havana exon 116347033 116347098 . - . gene_id "ENSMUSG00000052949"; gene_version "10"; transcript_id "ENSMUST00000137264"; transcript_version "1"; exon_number "2"; gene_name "Rnf157"; gene_source "ensembl_havana"; gene_biotype "protein_coding"; transcript_name "Rnf157-011"; transcript_source "havana"; transcript_biotype "processed_transcript"; exon_id "ENSMUSE00001212609"; exon_version "1"; transcript_support_level "3"; +11 havana exon 116345907 116345955 . - . gene_id "ENSMUSG00000052949"; gene_version "10"; transcript_id "ENSMUST00000137264"; transcript_version "1"; exon_number "3"; gene_name "Rnf157"; gene_source "ensembl_havana"; gene_biotype "protein_coding"; transcript_name "Rnf157-011"; transcript_source "havana"; transcript_biotype "processed_transcript"; exon_id "ENSMUSE00001217820"; exon_version "1"; transcript_support_level "3"; +11 havana exon 116340338 116340378 . - . gene_id "ENSMUSG00000052949"; gene_version "10"; transcript_id "ENSMUST00000137264"; transcript_version "1"; exon_number "4"; gene_name "Rnf157"; gene_source "ensembl_havana"; gene_biotype "protein_coding"; transcript_name "Rnf157-011"; transcript_source "havana"; transcript_biotype "processed_transcript"; exon_id "ENSMUSE00001233021"; exon_version "1"; transcript_support_level "3"; +11 havana exon 116338339 116338830 . - . gene_id "ENSMUSG00000052949"; gene_version "10"; transcript_id "ENSMUST00000137264"; transcript_version "1"; exon_number "5"; gene_name "Rnf157"; gene_source "ensembl_havana"; gene_biotype "protein_coding"; transcript_name "Rnf157-011"; transcript_source "havana"; transcript_biotype "processed_transcript"; exon_id "ENSMUSE00000796470"; exon_version "1"; transcript_support_level "3"; +2 havana transcript 128237693 128239643 . - . gene_id "ENSMUSG00000086879"; gene_version "1"; transcript_id "ENSMUST00000136025"; transcript_version "1"; gene_name "Gm14012"; gene_source "havana"; gene_biotype "lincRNA"; transcript_name "Gm14012-001"; transcript_source "havana"; transcript_biotype "lincRNA"; tag "basic"; transcript_support_level "1"; +2 havana exon 128239424 128239643 . - . gene_id "ENSMUSG00000086879"; gene_version "1"; transcript_id "ENSMUST00000136025"; transcript_version "1"; exon_number "1"; gene_name "Gm14012"; gene_source "havana"; gene_biotype "lincRNA"; transcript_name "Gm14012-001"; transcript_source "havana"; transcript_biotype "lincRNA"; exon_id "ENSMUSE00000756585"; exon_version "1"; tag "basic"; transcript_support_level "1"; +2 havana exon 128238858 128239058 . - . gene_id "ENSMUSG00000086879"; gene_version "1"; transcript_id "ENSMUST00000136025"; transcript_version "1"; exon_number "2"; gene_name "Gm14012"; gene_source "havana"; gene_biotype "lincRNA"; transcript_name "Gm14012-001"; transcript_source "havana"; transcript_biotype "lincRNA"; exon_id "ENSMUSE00000805740"; exon_version "1"; tag "basic"; transcript_support_level "1"; +2 havana exon 128238028 128238200 . - . gene_id "ENSMUSG00000086879"; gene_version "1"; transcript_id "ENSMUST00000136025"; transcript_version "1"; exon_number "3"; gene_name "Gm14012"; gene_source "havana"; gene_biotype "lincRNA"; transcript_name "Gm14012-001"; transcript_source "havana"; transcript_biotype "lincRNA"; exon_id "ENSMUSE00000818254"; exon_version "1"; tag "basic"; transcript_support_level "1"; +2 havana exon 128237693 128237775 . - . gene_id "ENSMUSG00000086879"; gene_version "1"; transcript_id "ENSMUST00000136025"; transcript_version "1"; exon_number "4"; gene_name "Gm14012"; gene_source "havana"; gene_biotype "lincRNA"; transcript_name "Gm14012-001"; transcript_source "havana"; transcript_biotype "lincRNA"; exon_id "ENSMUSE00000741865"; exon_version "1"; tag "basic"; transcript_support_level "1"; +17 havana transcript 39846958 39848788 . + . gene_id "ENSMUSG00000098178"; gene_version "1"; transcript_id "ENSMUST00000182010"; transcript_version "1"; gene_name "Yam1"; gene_source "havana"; gene_biotype "lincRNA"; transcript_name "Yam1-001"; transcript_source "havana"; transcript_biotype "lincRNA"; tag "basic"; transcript_support_level "NA"; +17 havana exon 39846958 39848788 . + . gene_id "ENSMUSG00000098178"; gene_version "1"; transcript_id "ENSMUST00000182010"; transcript_version "1"; exon_number "1"; gene_name "Yam1"; gene_source "havana"; gene_biotype "lincRNA"; transcript_name "Yam1-001"; transcript_source "havana"; transcript_biotype "lincRNA"; exon_id "ENSMUSE00001231387"; exon_version "1"; tag "basic"; transcript_support_level "NA"; +5 havana transcript 137134924 137142413 . - . gene_id "ENSMUSG00000037390"; gene_version "11"; transcript_id "ENSMUST00000160533"; transcript_version "3"; gene_name "Muc3"; gene_source "ensembl_havana"; gene_biotype "protein_coding"; transcript_name "Muc3-001"; transcript_source "havana"; transcript_biotype "protein_coding"; tag "cds_start_NF"; tag "mRNA_start_NF"; transcript_support_level "5 (assigned to previous version 2)"; +5 havana exon 137142259 137142413 . - . gene_id "ENSMUSG00000037390"; gene_version "11"; transcript_id "ENSMUST00000160533"; transcript_version "3"; exon_number "1"; gene_name "Muc3"; gene_source "ensembl_havana"; gene_biotype "protein_coding"; transcript_name "Muc3-001"; transcript_source "havana"; transcript_biotype "protein_coding"; exon_id "ENSMUSE00000310616"; exon_version "4"; tag "cds_start_NF"; tag "mRNA_start_NF"; transcript_support_level "5 (assigned to previous version 2)"; +5 havana CDS 137142259 137142413 . - 1 gene_id "ENSMUSG00000037390"; gene_version "11"; transcript_id "ENSMUST00000160533"; transcript_version "3"; exon_number "1"; gene_name "Muc3"; gene_source "ensembl_havana"; gene_biotype "protein_coding"; transcript_name "Muc3-001"; transcript_source "havana"; transcript_biotype "protein_coding"; protein_id "ENSMUSP00000123755"; protein_version "1"; tag "cds_start_NF"; tag "mRNA_start_NF"; transcript_support_level "5 (assigned to previous version 2)"; +5 havana exon 137141137 137141205 . - . gene_id "ENSMUSG00000037390"; gene_version "11"; transcript_id "ENSMUST00000160533"; transcript_version "3"; exon_number "2"; gene_name "Muc3"; gene_source "ensembl_havana"; gene_biotype "protein_coding"; transcript_name "Muc3-001"; transcript_source "havana"; transcript_biotype "protein_coding"; exon_id "ENSMUSE00000487002"; exon_version "2"; tag "cds_start_NF"; tag "mRNA_start_NF"; transcript_support_level "5 (assigned to previous version 2)"; +5 havana CDS 137141137 137141205 . - 2 gene_id "ENSMUSG00000037390"; gene_version "11"; transcript_id "ENSMUST00000160533"; transcript_version "3"; exon_number "2"; gene_name "Muc3"; gene_source "ensembl_havana"; gene_biotype "protein_coding"; transcript_name "Muc3-001"; transcript_source "havana"; transcript_biotype "protein_coding"; protein_id "ENSMUSP00000123755"; protein_version "1"; tag "cds_start_NF"; tag "mRNA_start_NF"; transcript_support_level "5 (assigned to previous version 2)"; +5 havana exon 137140691 137140853 . - . gene_id "ENSMUSG00000037390"; gene_version "11"; transcript_id "ENSMUST00000160533"; transcript_version "3"; exon_number "3"; gene_name "Muc3"; gene_source "ensembl_havana"; gene_biotype "protein_coding"; transcript_name "Muc3-001"; transcript_source "havana"; transcript_biotype "protein_coding"; exon_id "ENSMUSE00000488051"; exon_version "2"; tag "cds_start_NF"; tag "mRNA_start_NF"; transcript_support_level "5 (assigned to previous version 2)"; +5 havana CDS 137140691 137140853 . - 2 gene_id "ENSMUSG00000037390"; gene_version "11"; transcript_id "ENSMUST00000160533"; transcript_version "3"; exon_number "3"; gene_name "Muc3"; gene_source "ensembl_havana"; gene_biotype "protein_coding"; transcript_name "Muc3-001"; transcript_source "havana"; transcript_biotype "protein_coding"; protein_id "ENSMUSP00000123755"; protein_version "1"; tag "cds_start_NF"; tag "mRNA_start_NF"; transcript_support_level "5 (assigned to previous version 2)"; +5 havana exon 137138188 137138349 . - . gene_id "ENSMUSG00000037390"; gene_version "11"; transcript_id "ENSMUST00000160533"; transcript_version "3"; exon_number "4"; gene_name "Muc3"; gene_source "ensembl_havana"; gene_biotype "protein_coding"; transcript_name "Muc3-001"; transcript_source "havana"; transcript_biotype "protein_coding"; exon_id "ENSMUSE00001307390"; exon_version "1"; tag "cds_start_NF"; tag "mRNA_start_NF"; transcript_support_level "5 (assigned to previous version 2)"; +5 havana CDS 137138188 137138349 . - 1 gene_id "ENSMUSG00000037390"; gene_version "11"; transcript_id "ENSMUST00000160533"; transcript_version "3"; exon_number "4"; gene_name "Muc3"; gene_source "ensembl_havana"; gene_biotype "protein_coding"; transcript_name "Muc3-001"; transcript_source "havana"; transcript_biotype "protein_coding"; protein_id "ENSMUSP00000123755"; protein_version "1"; tag "cds_start_NF"; tag "mRNA_start_NF"; transcript_support_level "5 (assigned to previous version 2)"; +5 havana exon 137137925 137138022 . - . gene_id "ENSMUSG00000037390"; gene_version "11"; transcript_id "ENSMUST00000160533"; transcript_version "3"; exon_number "5"; gene_name "Muc3"; gene_source "ensembl_havana"; gene_biotype "protein_coding"; transcript_name "Muc3-001"; transcript_source "havana"; transcript_biotype "protein_coding"; exon_id "ENSMUSE00001251017"; exon_version "1"; tag "cds_start_NF"; tag "mRNA_start_NF"; transcript_support_level "5 (assigned to previous version 2)"; +5 havana CDS 137137925 137138022 . - 1 gene_id "ENSMUSG00000037390"; gene_version "11"; transcript_id "ENSMUST00000160533"; transcript_version "3"; exon_number "5"; gene_name "Muc3"; gene_source "ensembl_havana"; gene_biotype "protein_coding"; transcript_name "Muc3-001"; transcript_source "havana"; transcript_biotype "protein_coding"; protein_id "ENSMUSP00000123755"; protein_version "1"; tag "cds_start_NF"; tag "mRNA_start_NF"; transcript_support_level "5 (assigned to previous version 2)"; +5 havana exon 137137057 137137136 . - . gene_id "ENSMUSG00000037390"; gene_version "11"; transcript_id "ENSMUST00000160533"; transcript_version "3"; exon_number "6"; gene_name "Muc3"; gene_source "ensembl_havana"; gene_biotype "protein_coding"; transcript_name "Muc3-001"; transcript_source "havana"; transcript_biotype "protein_coding"; exon_id "ENSMUSE00001259522"; exon_version "1"; tag "cds_start_NF"; tag "mRNA_start_NF"; transcript_support_level "5 (assigned to previous version 2)"; +5 havana CDS 137137057 137137136 . - 2 gene_id "ENSMUSG00000037390"; gene_version "11"; transcript_id "ENSMUST00000160533"; transcript_version "3"; exon_number "6"; gene_name "Muc3"; gene_source "ensembl_havana"; gene_biotype "protein_coding"; transcript_name "Muc3-001"; transcript_source "havana"; transcript_biotype "protein_coding"; protein_id "ENSMUSP00000123755"; protein_version "1"; tag "cds_start_NF"; tag "mRNA_start_NF"; transcript_support_level "5 (assigned to previous version 2)"; +5 havana exon 137134924 137135485 . - . gene_id "ENSMUSG00000037390"; gene_version "11"; transcript_id "ENSMUST00000160533"; transcript_version "3"; exon_number "7"; gene_name "Muc3"; gene_source "ensembl_havana"; gene_biotype "protein_coding"; transcript_name "Muc3-001"; transcript_source "havana"; transcript_biotype "protein_coding"; exon_id "ENSMUSE00000869736"; exon_version "1"; tag "cds_start_NF"; tag "mRNA_start_NF"; transcript_support_level "5 (assigned to previous version 2)"; +5 havana CDS 137135444 137135485 . - 0 gene_id "ENSMUSG00000037390"; gene_version "11"; transcript_id "ENSMUST00000160533"; transcript_version "3"; exon_number "7"; gene_name "Muc3"; gene_source "ensembl_havana"; gene_biotype "protein_coding"; transcript_name "Muc3-001"; transcript_source "havana"; transcript_biotype "protein_coding"; protein_id "ENSMUSP00000123755"; protein_version "1"; tag "cds_start_NF"; tag "mRNA_start_NF"; transcript_support_level "5 (assigned to previous version 2)"; +5 havana UTR 137134924 137135443 . - . gene_id "ENSMUSG00000037390"; gene_version "11"; transcript_id "ENSMUST00000160533"; transcript_version "3"; gene_name "Muc3"; gene_source "ensembl_havana"; gene_biotype "protein_coding"; transcript_name "Muc3-001"; transcript_source "havana"; transcript_biotype "protein_coding"; tag "cds_start_NF"; tag "mRNA_start_NF"; transcript_support_level "5 (assigned to previous version 2)"; +17 ensembl_havana transcript 27655588 27711106 . + . gene_id "ENSMUSG00000040276"; gene_version "10"; transcript_id "ENSMUST00000045896"; transcript_version "5"; gene_name "Pacsin1"; gene_source "ensembl_havana"; gene_biotype "protein_coding"; transcript_name "Pacsin1-001"; transcript_source "ensembl_havana"; transcript_biotype "protein_coding"; tag "CCDS"; ccds_id "CCDS28567"; tag "basic"; transcript_support_level "1 (assigned to previous version 4)"; +17 ensembl_havana exon 27655588 27655759 . + . gene_id "ENSMUSG00000040276"; gene_version "10"; transcript_id "ENSMUST00000045896"; transcript_version "5"; exon_number "1"; gene_name "Pacsin1"; gene_source "ensembl_havana"; gene_biotype "protein_coding"; transcript_name "Pacsin1-001"; transcript_source "ensembl_havana"; transcript_biotype "protein_coding"; tag "CCDS"; ccds_id "CCDS28567"; exon_id "ENSMUSE00000460067"; exon_version "2"; tag "basic"; transcript_support_level "1 (assigned to previous version 4)"; +17 ensembl_havana exon 27701767 27701941 . + . gene_id "ENSMUSG00000040276"; gene_version "10"; transcript_id "ENSMUST00000045896"; transcript_version "5"; exon_number "2"; gene_name "Pacsin1"; gene_source "ensembl_havana"; gene_biotype "protein_coding"; transcript_name "Pacsin1-001"; transcript_source "ensembl_havana"; transcript_biotype "protein_coding"; tag "CCDS"; ccds_id "CCDS28567"; exon_id "ENSMUSE00000460051"; exon_version "1"; tag "basic"; transcript_support_level "1 (assigned to previous version 4)"; +17 ensembl_havana CDS 27701888 27701941 . + 0 gene_id "ENSMUSG00000040276"; gene_version "10"; transcript_id "ENSMUST00000045896"; transcript_version "5"; exon_number "2"; gene_name "Pacsin1"; gene_source "ensembl_havana"; gene_biotype "protein_coding"; transcript_name "Pacsin1-001"; transcript_source "ensembl_havana"; transcript_biotype "protein_coding"; tag "CCDS"; ccds_id "CCDS28567"; protein_id "ENSMUSP00000044168"; protein_version "3"; tag "basic"; transcript_support_level "1 (assigned to previous version 4)"; +17 ensembl_havana start_codon 27701888 27701890 . + 0 gene_id "ENSMUSG00000040276"; gene_version "10"; transcript_id "ENSMUST00000045896"; transcript_version "5"; exon_number "2"; gene_name "Pacsin1"; gene_source "ensembl_havana"; gene_biotype "protein_coding"; transcript_name "Pacsin1-001"; transcript_source "ensembl_havana"; transcript_biotype "protein_coding"; tag "CCDS"; ccds_id "CCDS28567"; tag "basic"; transcript_support_level "1 (assigned to previous version 4)"; +17 ensembl_havana exon 27702700 27702856 . + . gene_id "ENSMUSG00000040276"; gene_version "10"; transcript_id "ENSMUST00000045896"; transcript_version "5"; exon_number "3"; gene_name "Pacsin1"; gene_source "ensembl_havana"; gene_biotype "protein_coding"; transcript_name "Pacsin1-001"; transcript_source "ensembl_havana"; transcript_biotype "protein_coding"; tag "CCDS"; ccds_id "CCDS28567"; exon_id "ENSMUSE00000460033"; exon_version "1"; tag "basic"; transcript_support_level "1 (assigned to previous version 4)"; +17 ensembl_havana CDS 27702700 27702856 . + 0 gene_id "ENSMUSG00000040276"; gene_version "10"; transcript_id "ENSMUST00000045896"; transcript_version "5"; exon_number "3"; gene_name "Pacsin1"; gene_source "ensembl_havana"; gene_biotype "protein_coding"; transcript_name "Pacsin1-001"; transcript_source "ensembl_havana"; transcript_biotype "protein_coding"; tag "CCDS"; ccds_id "CCDS28567"; protein_id "ENSMUSP00000044168"; protein_version "3"; tag "basic"; transcript_support_level "1 (assigned to previous version 4)"; +17 ensembl_havana exon 27704845 27705080 . + . gene_id "ENSMUSG00000040276"; gene_version "10"; transcript_id "ENSMUST00000045896"; transcript_version "5"; exon_number "4"; gene_name "Pacsin1"; gene_source "ensembl_havana"; gene_biotype "protein_coding"; transcript_name "Pacsin1-001"; transcript_source "ensembl_havana"; transcript_biotype "protein_coding"; tag "CCDS"; ccds_id "CCDS28567"; exon_id "ENSMUSE00000460027"; exon_version "1"; tag "basic"; transcript_support_level "1 (assigned to previous version 4)"; +17 ensembl_havana CDS 27704845 27705080 . + 2 gene_id "ENSMUSG00000040276"; gene_version "10"; transcript_id "ENSMUST00000045896"; transcript_version "5"; exon_number "4"; gene_name "Pacsin1"; gene_source "ensembl_havana"; gene_biotype "protein_coding"; transcript_name "Pacsin1-001"; transcript_source "ensembl_havana"; transcript_biotype "protein_coding"; tag "CCDS"; ccds_id "CCDS28567"; protein_id "ENSMUSP00000044168"; protein_version "3"; tag "basic"; transcript_support_level "1 (assigned to previous version 4)"; +17 ensembl_havana exon 27705625 27705780 . + . gene_id "ENSMUSG00000040276"; gene_version "10"; transcript_id "ENSMUST00000045896"; transcript_version "5"; exon_number "5"; gene_name "Pacsin1"; gene_source "ensembl_havana"; gene_biotype "protein_coding"; transcript_name "Pacsin1-001"; transcript_source "ensembl_havana"; transcript_biotype "protein_coding"; tag "CCDS"; ccds_id "CCDS28567"; exon_id "ENSMUSE00000460023"; exon_version "1"; tag "basic"; transcript_support_level "1 (assigned to previous version 4)"; +17 ensembl_havana CDS 27705625 27705780 . + 0 gene_id "ENSMUSG00000040276"; gene_version "10"; transcript_id "ENSMUST00000045896"; transcript_version "5"; exon_number "5"; gene_name "Pacsin1"; gene_source "ensembl_havana"; gene_biotype "protein_coding"; transcript_name "Pacsin1-001"; transcript_source "ensembl_havana"; transcript_biotype "protein_coding"; tag "CCDS"; ccds_id "CCDS28567"; protein_id "ENSMUSP00000044168"; protein_version "3"; tag "basic"; transcript_support_level "1 (assigned to previous version 4)"; +17 ensembl_havana exon 27705917 27706092 . + . gene_id "ENSMUSG00000040276"; gene_version "10"; transcript_id "ENSMUST00000045896"; transcript_version "5"; exon_number "6"; gene_name "Pacsin1"; gene_source "ensembl_havana"; gene_biotype "protein_coding"; transcript_name "Pacsin1-001"; transcript_source "ensembl_havana"; transcript_biotype "protein_coding"; tag "CCDS"; ccds_id "CCDS28567"; exon_id "ENSMUSE00000460015"; exon_version "1"; tag "basic"; transcript_support_level "1 (assigned to previous version 4)"; +17 ensembl_havana CDS 27705917 27706092 . + 0 gene_id "ENSMUSG00000040276"; gene_version "10"; transcript_id "ENSMUST00000045896"; transcript_version "5"; exon_number "6"; gene_name "Pacsin1"; gene_source "ensembl_havana"; gene_biotype "protein_coding"; transcript_name "Pacsin1-001"; transcript_source "ensembl_havana"; transcript_biotype "protein_coding"; tag "CCDS"; ccds_id "CCDS28567"; protein_id "ENSMUSP00000044168"; protein_version "3"; tag "basic"; transcript_support_level "1 (assigned to previous version 4)"; +17 ensembl_havana exon 27706994 27707114 . + . gene_id "ENSMUSG00000040276"; gene_version "10"; transcript_id "ENSMUST00000045896"; transcript_version "5"; exon_number "7"; gene_name "Pacsin1"; gene_source "ensembl_havana"; gene_biotype "protein_coding"; transcript_name "Pacsin1-001"; transcript_source "ensembl_havana"; transcript_biotype "protein_coding"; tag "CCDS"; ccds_id "CCDS28567"; exon_id "ENSMUSE00000245756"; exon_version "2"; tag "basic"; transcript_support_level "1 (assigned to previous version 4)"; +17 ensembl_havana CDS 27706994 27707114 . + 1 gene_id "ENSMUSG00000040276"; gene_version "10"; transcript_id "ENSMUST00000045896"; transcript_version "5"; exon_number "7"; gene_name "Pacsin1"; gene_source "ensembl_havana"; gene_biotype "protein_coding"; transcript_name "Pacsin1-001"; transcript_source "ensembl_havana"; transcript_biotype "protein_coding"; tag "CCDS"; ccds_id "CCDS28567"; protein_id "ENSMUSP00000044168"; protein_version "3"; tag "basic"; transcript_support_level "1 (assigned to previous version 4)"; +17 ensembl_havana exon 27707219 27707346 . + . gene_id "ENSMUSG00000040276"; gene_version "10"; transcript_id "ENSMUST00000045896"; transcript_version "5"; exon_number "8"; gene_name "Pacsin1"; gene_source "ensembl_havana"; gene_biotype "protein_coding"; transcript_name "Pacsin1-001"; transcript_source "ensembl_havana"; transcript_biotype "protein_coding"; tag "CCDS"; ccds_id "CCDS28567"; exon_id "ENSMUSE00000245736"; exon_version "1"; tag "basic"; transcript_support_level "1 (assigned to previous version 4)"; +17 ensembl_havana CDS 27707219 27707346 . + 0 gene_id "ENSMUSG00000040276"; gene_version "10"; transcript_id "ENSMUST00000045896"; transcript_version "5"; exon_number "8"; gene_name "Pacsin1"; gene_source "ensembl_havana"; gene_biotype "protein_coding"; transcript_name "Pacsin1-001"; transcript_source "ensembl_havana"; transcript_biotype "protein_coding"; tag "CCDS"; ccds_id "CCDS28567"; protein_id "ENSMUSP00000044168"; protein_version "3"; tag "basic"; transcript_support_level "1 (assigned to previous version 4)"; +17 ensembl_havana exon 27707880 27708067 . + . gene_id "ENSMUSG00000040276"; gene_version "10"; transcript_id "ENSMUST00000045896"; transcript_version "5"; exon_number "9"; gene_name "Pacsin1"; gene_source "ensembl_havana"; gene_biotype "protein_coding"; transcript_name "Pacsin1-001"; transcript_source "ensembl_havana"; transcript_biotype "protein_coding"; tag "CCDS"; ccds_id "CCDS28567"; exon_id "ENSMUSE00000459997"; exon_version "1"; tag "basic"; transcript_support_level "1 (assigned to previous version 4)"; +17 ensembl_havana CDS 27707880 27708067 . + 1 gene_id "ENSMUSG00000040276"; gene_version "10"; transcript_id "ENSMUST00000045896"; transcript_version "5"; exon_number "9"; gene_name "Pacsin1"; gene_source "ensembl_havana"; gene_biotype "protein_coding"; transcript_name "Pacsin1-001"; transcript_source "ensembl_havana"; transcript_biotype "protein_coding"; tag "CCDS"; ccds_id "CCDS28567"; protein_id "ENSMUSP00000044168"; protein_version "3"; tag "basic"; transcript_support_level "1 (assigned to previous version 4)"; +17 ensembl_havana exon 27708426 27711106 . + . gene_id "ENSMUSG00000040276"; gene_version "10"; transcript_id "ENSMUST00000045896"; transcript_version "5"; exon_number "10"; gene_name "Pacsin1"; gene_source "ensembl_havana"; gene_biotype "protein_coding"; transcript_name "Pacsin1-001"; transcript_source "ensembl_havana"; transcript_biotype "protein_coding"; tag "CCDS"; ccds_id "CCDS28567"; exon_id "ENSMUSE00000348638"; exon_version "2"; tag "basic"; transcript_support_level "1 (assigned to previous version 4)"; +17 ensembl_havana CDS 27708426 27708532 . + 2 gene_id "ENSMUSG00000040276"; gene_version "10"; transcript_id "ENSMUST00000045896"; transcript_version "5"; exon_number "10"; gene_name "Pacsin1"; gene_source "ensembl_havana"; gene_biotype "protein_coding"; transcript_name "Pacsin1-001"; transcript_source "ensembl_havana"; transcript_biotype "protein_coding"; tag "CCDS"; ccds_id "CCDS28567"; protein_id "ENSMUSP00000044168"; protein_version "3"; tag "basic"; transcript_support_level "1 (assigned to previous version 4)"; +17 ensembl_havana stop_codon 27708533 27708535 . + 0 gene_id "ENSMUSG00000040276"; gene_version "10"; transcript_id "ENSMUST00000045896"; transcript_version "5"; exon_number "10"; gene_name "Pacsin1"; gene_source "ensembl_havana"; gene_biotype "protein_coding"; transcript_name "Pacsin1-001"; transcript_source "ensembl_havana"; transcript_biotype "protein_coding"; tag "CCDS"; ccds_id "CCDS28567"; tag "basic"; transcript_support_level "1 (assigned to previous version 4)"; +17 ensembl_havana UTR 27655588 27655759 . + . gene_id "ENSMUSG00000040276"; gene_version "10"; transcript_id "ENSMUST00000045896"; transcript_version "5"; gene_name "Pacsin1"; gene_source "ensembl_havana"; gene_biotype "protein_coding"; transcript_name "Pacsin1-001"; transcript_source "ensembl_havana"; transcript_biotype "protein_coding"; tag "CCDS"; ccds_id "CCDS28567"; tag "basic"; transcript_support_level "1 (assigned to previous version 4)"; +17 ensembl_havana UTR 27701767 27701887 . + . gene_id "ENSMUSG00000040276"; gene_version "10"; transcript_id "ENSMUST00000045896"; transcript_version "5"; gene_name "Pacsin1"; gene_source "ensembl_havana"; gene_biotype "protein_coding"; transcript_name "Pacsin1-001"; transcript_source "ensembl_havana"; transcript_biotype "protein_coding"; tag "CCDS"; ccds_id "CCDS28567"; tag "basic"; transcript_support_level "1 (assigned to previous version 4)"; +17 ensembl_havana UTR 27708536 27711106 . + . gene_id "ENSMUSG00000040276"; gene_version "10"; transcript_id "ENSMUST00000045896"; transcript_version "5"; gene_name "Pacsin1"; gene_source "ensembl_havana"; gene_biotype "protein_coding"; transcript_name "Pacsin1-001"; transcript_source "ensembl_havana"; transcript_biotype "protein_coding"; tag "CCDS"; ccds_id "CCDS28567"; tag "basic"; transcript_support_level "1 (assigned to previous version 4)";
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/chimeras Sun Jan 19 16:31:34 2020 -0500 @@ -0,0 +1,3 @@ +tagid txid1 txid2 geneid1 geneid2 symbol1 symbol2 region1 region2 tx_pos_start1 tx_pos_end1 tx_pos_strand1 length1 tx_pos_start2 tx_pos_end2 tx_pos_strand2 length2 read_info genomic_pos1 genomic_pos2 locus1 locus2 groupid1 groupid2 tpm1 tpm2 score1 score2 score sequences hybrid hybrid_pos mfe +4|1 ENSMUST00000136025 mmu-miR-6979-3p NA NA NA NA NA NA 32 46 + NA 2 12 + NA 2,15,30,39,54 ENSMUST00000136025:32:46:+ mmu-miR-6979-3p:2:12:+ ENSMUST00000136025:32:46:+ mmu-miR-6979-3p:2:12:+ 1 7 121100.0 165100.0 1.0 1.0 2.0 CAGGACUCUUGGCU&GUGUCUGUCU NA NA NA +3|2 mmu-miR-20a-5p ENSMUST00000136025 NA NA NA NA NA NA 0 23 + NA 132 142 + NA 6,28,35,44,55 mmu-miR-20a-5p:0:23:+ ENSMUST00000136025:132:142:+ mmu-miR-20a-5p:0:23:+ ENSMUST00000136025:132:142:+ 5 2 75660.0 165100.0 1.0 1.0 2.0 UAAAGUGCUUAUAGUGCAGGUAG&CUGCCUGCCU ((.((((((((&)))))))))) 13&1 -10.88
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/loci.counts Sun Jan 19 16:31:34 2020 -0500 @@ -0,0 +1,9 @@ +6|1|1 ENSMUST00000182010 4 0 19 68 + 5S49M1S ENSMUST00000182010:19:68:+ ENSMUST00000182010:19:74:+ 1 1 6.485e+04 +7|9|1 ENSMUST00000182010 4 0 24 74 + 5S50M ENSMUST00000182010:24:74:+ ENSMUST00000182010:19:74:+ 1 1 6.485e+04 +4|1|2 ENSMUST00000136025 0 1 32 46 + 1S14M39S ENSMUST00000136025:32:46:+ ENSMUST00000136025:32:46:+ 1 1 1.211e+05 +3|2|2 ENSMUST00000136025 1 2 132 142 + 34S10M11S ENSMUST00000136025:132:142:+ ENSMUST00000136025:132:142:+ 1 1 1.651e+05 +2|2|2 ENSMUST00000137264 2 3 12 27 + 4S15M30S ENSMUST00000137264:12:27:+ ENSMUST00000137264:12:27:+ 1 1 1.135e+05 +6|1|4 ENSMUST00000160533 3 4 69 82 + 42S13M ENSMUST00000160533:69:82:+ ENSMUST00000160533:69:82:+ 1 1 1.297e+05 +3|2|1 mmu-miR-20a-5p 5 5 0 23 + 5S23M27S mmu-miR-20a-5p:0:23:+ mmu-miR-20a-5p:0:23:+ 1 1 7.566e+04 +2|2|1 mmu-miR-6898-5p 6 6 11 21 + 10M39S mmu-miR-6898-5p:11:21:+ mmu-miR-6898-5p:11:21:+ 1 1 1.651e+05 +4|1|1 mmu-miR-6979-3p 7 7 2 12 + 29S10M15S mmu-miR-6979-3p:2:12:+ mmu-miR-6979-3p:2:12:+ 1 1 1.651e+05
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/merged.bed Sun Jan 19 16:31:34 2020 -0500 @@ -0,0 +1,8 @@ +ENSMUST00000136025 32 46 + 4|1|2,ENSMUST00000136025,32,46,+,1S14M39S +ENSMUST00000136025 132 142 + 3|2|2,ENSMUST00000136025,132,142,+,34S10M11S +ENSMUST00000137264 12 27 + 2|2|2,ENSMUST00000137264,12,27,+,4S15M30S +ENSMUST00000160533 69 82 + 6|1|4,ENSMUST00000160533,69,82,+,42S13M +ENSMUST00000182010 19 74 + 6|1|1,ENSMUST00000182010,19,68,+,5S49M1S;7|9|1,ENSMUST00000182010,24,74,+,5S50M +mmu-miR-20a-5p 0 23 + 3|2|1,mmu-miR-20a-5p,0,23,+,5S23M27S +mmu-miR-6898-5p 11 21 + 2|2|1,mmu-miR-6898-5p,11,21,+,10M39S +mmu-miR-6979-3p 2 12 + 4|1|1,mmu-miR-6979-3p,2,12,+,29S10M15S
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/reads.fasta Sun Jan 19 16:31:34 2020 -0500 @@ -0,0 +1,14 @@ +>1|1 +AAAAGACTCTGTAGACATGGCTGGTCTTGAACTCACAGAGATTTGTCTGCCTTTC +>2|2 +ATGCAGGGAGGACGATGCGAATGGGGAATCAGGCTAAACAGTTTGAATA +>3|2 +CGCGGTAAAGTGCTTATAGTGCAGGTAGACAGTACTGCCTGCCTTAATGAATGAA +>4|1 +TCAGGACTCTTGGCTGTATCCTTTCCTAAGTGTCTGTCTTCCTTACCACTAACA +>5|8 +TGTAGACACAAGGTACACCAATGCCTCTCTGACCACCCATGAGTCTTTGACCTA +>6|1 +TGTGGTCATTAATCAAGAACGAAAGTCGGAGGTTCGAAGACGATTAGATACCGTC +>7|9 +TGTTGAATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGT
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/reads.fastq Sun Jan 19 16:31:34 2020 -0500 @@ -0,0 +1,96 @@ +@183438/1 +TGTTGAATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGT ++ +GGGGHHHHHHHHHHHGGGGGHHGGGGGGHHGHHGGHGGGHGHHHHHHGGGGGHGG +@397634/1 +TGTTGAATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGT ++ +GGGGHHGHHHHHHHHGGGGGHHGGGGGGHHGHHGGHGGGHGHHHHHHGGGGGHGG +@701890/1 +TGTTGAATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGT ++ +EEGGHGHHHHHHHHHGGGGGGHGGCGGFGHGHHGGHGGGHGHHHHHHGGGGGGFG +@745414/1 +TGTTGAATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGT ++ +GGGGHHHHHHHHHHHGGGGGHHGGGGGGHHGHHGGHGGGHGHHHHHHGGGGGHGF +@753363/1 +TGTTGAATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGT ++ +EEEGHHHFHHHHHHHGGFEEFGGGGGGGHHGHHGGHGGFHGHHHHHHGFGGGGGG +@881071/1 +TGTTGAATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGT ++ +GGGGHHHHHHHHHHHGGGGGHHGGFGGGHHGHHGGHGGGHGHHHHHHGFGGFHGG +@1059959/1 +TGTTGAATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGT ++ +GGGGHHHHHHHHHHHGGGGGHHGGGGGEHHGHHGGHGGGHGHHHHHHGGGGGHGG +@1157152/1 +TGTTGAATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGT ++ +GGGGHHHHHHHHHHHGGGGGHHGGGGGEHHGHHGGHGGGHGHHHHHHGGGGGHGG +@1295751/1 +TGTTGAATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGT ++ +GGGGHHHHHHHHHHHGGGGGHHGGGGGGHHGHHGGHGGGHGHHHHHHGGGGGHGG +@585749/1 +TGTGGTCATTAATCAAGAACGAAAGTCGGAGGTTCGAAGACGATTAGATACCGTC ++ +GGEGGHHHHHHHHHHHFHHHG?FGEHHGGGFEEHHGHHGGFF?FHGHHHHHGEGG +@1209323/1 +ATGCAGGGAGGACGATGCGAATGGGGAATCAGGCTAAACAGTTTGAATA ++ +3AAABFFBBBBBGGEFGGG2AAGHHGGAGHHHHHHHHCHGHHHHHH5GF +@1248864/1 +ATGCAGGGAGGACGATGCGAATGGGGAATCAGGCTAAACAGTTTGAATA ++ +ABBBAF4DAB?BGGEFEFG2AAF3CEGGHHGGFCG33FHFHFHHHHHHH +@475998/1 +CGCGGTAAAGTGCTTATAGTGCAGGTAGACAGTACTGCCTGCCTTAATGAATGAA ++ +GGEEGB1FFFDGGGFHHHDDFGGHFHEFHHHHFDGGGE?GHFHHHHFHHHDEDHD +@478112/1 +CGCGGTAAAGTGCTTATAGTGCAGGTAGACAGTACTGCCTGCCTTAATGAATGAA ++ +EEFGGFGEHHFGHBBFHHHFGHHHHAGHHHFHFFGEF@BFGGAEHFFHHHGHGHF +@74093/1 +TGTAGACACAAGGTACACCAATGCCTCTCTGACCACCCATGAGTCTTTGACCTA ++ +G?FHHFFHFEFHH3FBAGGC2AAFEEGHFGFDEGGGFEAGFGE5AFF5@@BE5G +@448633/1 +TGTAGACACAAGGTACACCAATGCCTCTCTGACCACCCATGAGTCTTTGACCTA ++ +EFGGFHH5BAFA23AAFFHHDGGHHFGHHHEGHCFGFAFGH3FGGBBG@E@FGF +@849624/1 +TGTAGACACAAGGTACACCAATGCCTCTCTGACCACCCATGAGTCTTTGACCTA ++ +AABFBFFFF2CGDBADEFB2FAFFFHBGHFHDFGHAEGCFFEFDD5GGG@BBFE +@1076923/1 +TGTAGACACAAGGTACACCAATGCCTCTCTGACCACCCATGAGTCTTTGACCTA ++ +GCEFGHHHFEFH23DB553A2BGFFDBBA35FGFFGGGEECGH5DD@@@EBEGF +@1278542/1 +TGTAGACACAAGGTACACCAATGCCTCTCTGACCACCCATGAGTCTTTGACCTA ++ +EGGFHGHHHHGHGEGFHHHHHHFHHHFHGFGHHHHHGGEE3GHGGHGFDBGHHE +@1290525/1 +TGTAGACACAAGGTACACCAATGCCTCTCTGACCACCCATGAGTCTTTGACCTA ++ +CEFFDHH5B2AE2F555532AGF3EGH3AFAEHHFHGD133335DFGH5D@3FF +@1379287/1 +TGTAGACACAAGGTACACCAATGCCTCTCTGACCACCCATGAGTCTTTGACCTA ++ +AEEF1FGHHHGHHFGHHHGG/E1EHHHHGHHFDGGEG?CFGBGEGHHGHHHHHH +@1490590/1 +TGTAGACACAAGGTACACCAATGCCTCTCTGACCACCCATGAGTCTTTGACCTA ++ +GEA3BD5DF2EGCGEDF5EGH3AGHHHFHGFBFHBGGGGH33BFFD555D53FG +@854752/1 +AAAAGACTCTGTAGACATGGCTGGTCTTGAACTCACAGAGATTTGTCTGCCTTTC ++ +G?EFFAF5335DD5B5553222B1A55AFAGHHE33BAF325555A5DF5B3F3B +@1180232/1 +TCAGGACTCTTGGCTGTATCCTTTCCTAAGTGTCTGTCTTCCTTACCACTAACA ++ +EGE0F10B01DDBGC/0BFFGGHHHFE21BA2D221DGHH2DBAGHBDFBG1GF
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/ref.fasta Sun Jan 19 16:31:34 2020 -0500 @@ -0,0 +1,18 @@ +>mmu-miR-20a-5p +TAAAGTGCTTATAGTGCAGGTAG +>mmu-miR-802-3p +ACGGAGAGTCTTTGTCACTCAG +>mmu-miR-6898-5p +TGTAAGGGGAGATGCAGGGAGC +>mmu-miR-6979-3p +TTGTGTCTGTCTGGCTCCCAG +>ENSMUST00000137264 +CTACTCACATGAAGGGAGGACGATGCGCAACCCTCCACCCACCTGCCTCATCGCCTGTAACCGCGATTCTTACCCTCTCAGAAAGAACCAGAAGCCTTCCCTCTGTGGGCTAATACGTGCCAGCCAAACCTGTGGGCCAGCTGAGCTGAGGGTCAGGGCTGGTTGTTTCTGTAGGCTTTCTCTTCTGAGTGGAGACCAAGAAGACTGAAGAGTTTGGCAGCCTTGTTAGACGCCCATGGACAGGTTTTGGTCTCTGGCACTC +>ENSMUST00000136025 +GATCTGATTGAGTCACAGCTGAGATGTGGCACCAGGACTCTTGGCTACACTCAGTTCTCTCAGTTTGACTGAGTACTCTGGGATCACAGAAGTGACTACATGATGCAGCCCAGAGGTCTGAACTCAAAGCTTCTGCCTGCCTACCTTATCTCACTGCTGGCAAATGCATCCCCTCTGCTCCACCCATC +>ENSMUST00000182010 +GCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAGTCGGAGTTTCGAAGACGATCAGATACCGTTGTAGTTCCAACCATAAACGATGCCGACTGGC +>ENSMUST00000160533 +CAAAGGCAGTATCGTGGTGGATTATGATGTCATCCTGAAGGCCAAGTACACCCCAGGATTTGAAAACACATTAGATACCGTCGTCAAAAACCTGGAGACA +>ENSMUST00000045896 +GAGGCCAATGGTGGCGCCAACCCCTTCGAGGATGATGCCAAGGGAGTTCGTGTACGGGCACTCTATGACTACGACGGTCAGGAGCAGGATGAGCTCAGCT
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/ref1.fasta Sun Jan 19 16:31:34 2020 -0500 @@ -0,0 +1,8 @@ +>mmu-miR-20a-5p +TAAAGTGCTTATAGTGCAGGTAG +>mmu-miR-802-3p +ACGGAGAGTCTTTGTCACTCAG +>mmu-miR-6898-5p +TGTAAGGGGAGATGCAGGGAGC +>mmu-miR-6979-3p +TTGTGTCTGTCTGGCTCCCAG
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/ref2.fasta Sun Jan 19 16:31:34 2020 -0500 @@ -0,0 +1,10 @@ +>ENSMUST00000137264 +CTACTCACATGAAGGGAGGACGATGCGCAACCCTCCACCCACCTGCCTCATCGCCTGTAACCGCGATTCTTACCCTCTCAGAAAGAACCAGAAGCCTTCCCTCTGTGGGCTAATACGTGCCAGCCAAACCTGTGGGCCAGCTGAGCTGAGGGTCAGGGCTGGTTGTTTCTGTAGGCTTTCTCTTCTGAGTGGAGACCAAGAAGACTGAAGAGTTTGGCAGCCTTGTTAGACGCCCATGGACAGGTTTTGGTCTCTGGCACTC +>ENSMUST00000136025 +GATCTGATTGAGTCACAGCTGAGATGTGGCACCAGGACTCTTGGCTACACTCAGTTCTCTCAGTTTGACTGAGTACTCTGGGATCACAGAAGTGACTACATGATGCAGCCCAGAGGTCTGAACTCAAAGCTTCTGCCTGCCTACCTTATCTCACTGCTGGCAAATGCATCCCCTCTGCTCCACCCATC +>ENSMUST00000182010 +GCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAGTCGGAGTTTCGAAGACGATCAGATACCGTTGTAGTTCCAACCATAAACGATGCCGACTGGC +>ENSMUST00000160533 +CAAAGGCAGTATCGTGGTGGATTATGATGTCATCCTGAAGGCCAAGTACACCCCAGGATTTGAAAACACATTAGATACCGTCGTCAAAAACCTGGAGACA +>ENSMUST00000045896 +GAGGCCAATGGTGGCGCCAACCCCTTCGAGGATGATGCCAAGGGAGTTCGTGTACGGGCACTCTATGACTACGACGGTCAGGAGCAGGATGAGCTCAGCT
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/segments.bed Sun Jan 19 16:31:34 2020 -0500 @@ -0,0 +1,13 @@ +ENSMUST00000182010 19 68 6|1|1,ENSMUST00000182010,19,68,+,5S49M1S 0 + +ENSMUST00000182010 24 74 7|9|1,ENSMUST00000182010,24,74,+,5S50M 0 + +mmu-miR-20a-5p 0 23 3|2|1,mmu-miR-20a-5p,0,23,+,5S23M27S 0 + +mmu-miR-6898-5p 11 21 2|2|1,mmu-miR-6898-5p,11,21,+,10M39S 0 + +mmu-miR-6979-3p 2 12 4|1|1,mmu-miR-6979-3p,2,12,+,29S10M15S 0 + +ENSMUST00000137264 12 27 2|2|2,ENSMUST00000137264,12,27,+,4S15M30S 0 + +ENSMUST00000136025 32 46 4|1|2,ENSMUST00000136025,32,46,+,1S14M39S 0 + +ENSMUST00000136025 132 142 3|2|2,ENSMUST00000136025,132,142,+,34S10M11S 0 + +ENSMUST00000182010 19 45 6|1|2,ENSMUST00000182010,19,45,+,5S26M24S 0 + +ENSMUST00000182010 24 45 7|9|2,ENSMUST00000182010,24,45,+,5S21M29S 0 + +ENSMUST00000182010 46 58 6|1|3,ENSMUST00000182010,46,58,+,32S12M11S 0 + +ENSMUST00000182010 46 68 7|9|3,ENSMUST00000182010,46,68,+,27S22M6S 0 + +ENSMUST00000160533 69 82 6|1|4,ENSMUST00000160533,69,82,+,42S13M 0 +
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/singletons Sun Jan 19 16:31:34 2020 -0500 @@ -0,0 +1,4 @@ +tagid txid geneid symbol region tx_pos_start tx_pos_end tx_pos_strand length read_info genomic_pos locus groupid tpm score score sequences +6|1 ENSMUST00000182010 NA NA NA 19 68 + NA 6,54,55 ENSMUST00000182010:19:68:+ ENSMUST00000182010:19:74:+ 0 64850.0 1.0 1.0 UCAUUAAUCAAGAACGAAAGUCGGAGUUUCGAAGACGAUCAGAUACCGUUGUAGU +7|9 ENSMUST00000182010 NA NA NA 24 74 + NA 6,55,55 ENSMUST00000182010:24:74:+ ENSMUST00000182010:19:74:+ 0 64850.0 1.0 1.0 UCAUUAAUCAAGAACGAAAGUCGGAGUUUCGAAGACGAUCAGAUACCGUUGUAGU +2|2 ENSMUST00000137264 NA NA NA 12 27 + NA 5,19,49 ENSMUST00000137264:12:27:+ ENSMUST00000137264:12:27:+ 3 113500.0 1.0 1.0 AGGGAGGACGAUGCG
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/tool_data_table_conf.xml.sample Sun Jan 19 16:31:34 2020 -0500 @@ -0,0 +1,7 @@ +<tables> + <!-- Locations of all fasta files under genome directory --> + <table name="all_fasta" comment_char="#"> + <columns>value, dbkey, name, path</columns> + <file path="all_fasta.loc" /> + </table> +</tables>