Mercurial > repos > iuc > colibread_mapsembler2
annotate mapsembler2.xml @ 0:265fa5effd4d draft
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/colibread commit a213833526146246d277ec7851165971449b501e
author | iuc |
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date | Fri, 20 Oct 2017 03:15:10 -0400 |
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children | 00fd0b4274fd |
rev | line source |
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0
265fa5effd4d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/colibread commit a213833526146246d277ec7851165971449b501e
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1 <?xml version='1.0' encoding='utf-8'?> |
265fa5effd4d
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2 <tool profile="16.04" id="mapsembler2" name="Mapsembler2" version="2.2.4"> |
265fa5effd4d
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3 <description>is a targeted assembly software</description> |
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4 <macros> |
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5 <import>macros.xml</import> |
265fa5effd4d
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6 </macros> |
265fa5effd4d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/colibread commit a213833526146246d277ec7851165971449b501e
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7 <requirements> |
265fa5effd4d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/colibread commit a213833526146246d277ec7851165971449b501e
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8 <requirement type="package" version="2.2.4">mapsembler2</requirement> |
265fa5effd4d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/colibread commit a213833526146246d277ec7851165971449b501e
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9 </requirements> |
265fa5effd4d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/colibread commit a213833526146246d277ec7851165971449b501e
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10 <command><![CDATA[ |
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11 |
265fa5effd4d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/colibread commit a213833526146246d277ec7851165971449b501e
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12 #set $starter_filename = str($s) + "." + $s.ext |
265fa5effd4d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/colibread commit a213833526146246d277ec7851165971449b501e
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13 ln -sf '${s}' '${starter_filename}' && |
265fa5effd4d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/colibread commit a213833526146246d277ec7851165971449b501e
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14 |
265fa5effd4d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/colibread commit a213833526146246d277ec7851165971449b501e
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15 #set $samples = [] |
265fa5effd4d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/colibread commit a213833526146246d277ec7851165971449b501e
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16 #for $read in $r |
265fa5effd4d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/colibread commit a213833526146246d277ec7851165971449b501e
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17 #set $filename = str($read) + "." + $read.ext |
265fa5effd4d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/colibread commit a213833526146246d277ec7851165971449b501e
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18 ln -sf '${read}' '${filename}' && |
265fa5effd4d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/colibread commit a213833526146246d277ec7851165971449b501e
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19 #silent $samples.append($filename) |
265fa5effd4d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/colibread commit a213833526146246d277ec7851165971449b501e
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20 #end for |
265fa5effd4d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/colibread commit a213833526146246d277ec7851165971449b501e
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21 |
265fa5effd4d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/colibread commit a213833526146246d277ec7851165971449b501e
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22 run_mapsembler2_pipeline.sh |
265fa5effd4d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/colibread commit a213833526146246d277ec7851165971449b501e
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23 -s '${starter_filename}' |
265fa5effd4d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/colibread commit a213833526146246d277ec7851165971449b501e
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24 -r "${ ' '.join(['%s' % read for read in $samples]) }" |
265fa5effd4d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/colibread commit a213833526146246d277ec7851165971449b501e
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25 -t ${t} |
265fa5effd4d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/colibread commit a213833526146246d277ec7851165971449b501e
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26 -k ${k} |
265fa5effd4d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/colibread commit a213833526146246d277ec7851165971449b501e
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27 -c ${c} |
265fa5effd4d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/colibread commit a213833526146246d277ec7851165971449b501e
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28 -d ${d} |
265fa5effd4d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/colibread commit a213833526146246d277ec7851165971449b501e
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29 -g ${g} |
265fa5effd4d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/colibread commit a213833526146246d277ec7851165971449b501e
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30 -f ${f} |
265fa5effd4d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/colibread commit a213833526146246d277ec7851165971449b501e
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31 -x ${x} |
265fa5effd4d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/colibread commit a213833526146246d277ec7851165971449b501e
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32 -y ${y} |
265fa5effd4d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/colibread commit a213833526146246d277ec7851165971449b501e
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33 ]]></command> |
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34 |
265fa5effd4d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/colibread commit a213833526146246d277ec7851165971449b501e
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35 <inputs> |
265fa5effd4d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/colibread commit a213833526146246d277ec7851165971449b501e
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36 <!-- Input data files --> |
265fa5effd4d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/colibread commit a213833526146246d277ec7851165971449b501e
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37 <param argument="-s" type="data" format="fasta" label="Starters" help="Set of input sequences" /> |
265fa5effd4d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/colibread commit a213833526146246d277ec7851165971449b501e
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38 <param argument="-r" type="data" multiple="true" format="fasta,fastq" label="List of reads" /> |
265fa5effd4d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/colibread commit a213833526146246d277ec7851165971449b501e
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39 <param argument="-t" type="select" label="Select your output extension type"> |
265fa5effd4d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/colibread commit a213833526146246d277ec7851165971449b501e
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40 <option value="1">a strict sequence</option> |
265fa5effd4d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/colibread commit a213833526146246d277ec7851165971449b501e
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41 <option value="2">a consensus sequence</option> |
265fa5effd4d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/colibread commit a213833526146246d277ec7851165971449b501e
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42 </param> |
265fa5effd4d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/colibread commit a213833526146246d277ec7851165971449b501e
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43 <param argument="-k" type="integer" label="Size of kmers" value="31" help="Set the length of used kmers. Must fit the compiled value. Only uneven number" /> |
265fa5effd4d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/colibread commit a213833526146246d277ec7851165971449b501e
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44 <param argument="-c" type="integer" label="Minimal coverage" value="5" help="Set the minimal coverage: Used by Phaser (don't use kmers with lower coverage) "/> |
265fa5effd4d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/colibread commit a213833526146246d277ec7851165971449b501e
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45 <param argument="-d" type="integer" label="Number of authorized substitutions" value="1" help="Set the number of authorized substitutions used while mapping reads on finding SNPs"/> |
265fa5effd4d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/colibread commit a213833526146246d277ec7851165971449b501e
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46 <param argument="-g" type="integer" label="Estimated genome size" value="10000000" help="Used only to control memory usage. e.g.3 billion (3000000000) uses 4Gb of RAM." /> |
265fa5effd4d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/colibread commit a213833526146246d277ec7851165971449b501e
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47 <param argument="-f" type="select" label="Process of search" help="Set the process of search in the graph" > |
265fa5effd4d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/colibread commit a213833526146246d277ec7851165971449b501e
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48 <option value="1">Breadth</option> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/colibread commit a213833526146246d277ec7851165971449b501e
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49 <option value="2">Depth</option> |
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50 </param> |
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51 <param argument="-x" type="integer" label="Max length of nodes" value="40" help="Set the maximal length of nodes"/> |
265fa5effd4d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/colibread commit a213833526146246d277ec7851165971449b501e
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52 <param argument="-y" type="integer" label="Max depth of nodes" value="10000" help="Set the maximal depth of the graph"/> |
265fa5effd4d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/colibread commit a213833526146246d277ec7851165971449b501e
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53 </inputs> |
265fa5effd4d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/colibread commit a213833526146246d277ec7851165971449b501e
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54 |
265fa5effd4d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/colibread commit a213833526146246d277ec7851165971449b501e
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55 <outputs> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/colibread commit a213833526146246d277ec7851165971449b501e
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56 <data name="fasta" from_work_dir="res_k_*.fasta" format="fasta" label="Assembly with ${tool.name} on $on_string"/> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/colibread commit a213833526146246d277ec7851165971449b501e
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57 <data name="coherent" from_work_dir="res_coherent_*.fasta" format="fasta" label="Coherent with ${tool.name} on $on_string" hidden="true"/> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/colibread commit a213833526146246d277ec7851165971449b501e
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58 <data name="uncoherent" from_work_dir="res_uncoherent_*.fasta" format="fasta" label="Uncoherent with ${tool.name} on $on_string" hidden="true"/> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/colibread commit a213833526146246d277ec7851165971449b501e
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59 <data name="extremities" from_work_dir="starter_extremities.fa" format="fasta" label="Starter extremities with ${tool.name} on $on_string" hidden="true"/> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/colibread commit a213833526146246d277ec7851165971449b501e
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60 </outputs> |
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61 |
265fa5effd4d
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62 <tests> |
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63 <test> |
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64 <param name="s" value="mapsembler2/starter.fa" /> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/colibread commit a213833526146246d277ec7851165971449b501e
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65 <param name="r" value="mapsembler2/reads1,mapsembler2/reads2" /> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/colibread commit a213833526146246d277ec7851165971449b501e
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66 <param name="t" value="2"/> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/colibread commit a213833526146246d277ec7851165971449b501e
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67 <output name="fasta" file="mapsembler2/assembly.fa"/> |
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68 </test> |
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69 <test> |
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70 <param name="s" value="mapsembler2/starter.fa" /> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/colibread commit a213833526146246d277ec7851165971449b501e
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71 <param name="r" value="mapsembler2/reads2,mapsembler2/reads1" /> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/colibread commit a213833526146246d277ec7851165971449b501e
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72 <param name="t" value="1"/> |
265fa5effd4d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/colibread commit a213833526146246d277ec7851165971449b501e
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73 <output name="fasta" file="mapsembler2/assembly_2.fa"/> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/colibread commit a213833526146246d277ec7851165971449b501e
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74 <output name="coherent" file="mapsembler2/coherent.fa"/> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/colibread commit a213833526146246d277ec7851165971449b501e
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75 <output name="extremities" file="mapsembler2/starter_extremities.fa"/> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/colibread commit a213833526146246d277ec7851165971449b501e
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76 </test> |
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77 </tests> |
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78 |
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79 <help><![CDATA[ |
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80 |
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81 **Description** |
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82 |
265fa5effd4d
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/colibread commit a213833526146246d277ec7851165971449b501e
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83 Mapsembler2 is a targeted assembly software. It takes as input a set of NGS raw reads (fasta or fastq, gzipped or not) and a set of input sequences (starters). It first determines if each starter is read-coherent, e.g. whether reads confirm the presence of each starter in the original sequence. Then for each read-coherent starter, Mapsembler2 outputs its sequence neighborhood as a linear sequence or as a graph, depending on the user choice. |
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84 Mapsembler2 may be used for (not limited to): |
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85 |
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86 · Validate an assembled sequence (input as starter), e.g. from a de Bruijn graph assembly where read-coherence was not enforced. |
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87 |
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88 · Checks if a gene (input as starter) has an homolog in a set of reads. |
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89 |
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90 · Checks if a known enzyme is present in a metagenomic NGS read set. |
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91 |
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92 · Enrich unmappable reads by extending them, possibly making them mappable. |
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93 |
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94 · Checks what happens at the extremities of a contig. |
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95 |
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96 · Remove contaminants or symbiont reads from a read set |
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97 |
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98 ------- |
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99 |
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100 **Web site** |
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101 |
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102 http://colibread.inria.fr/mapsembler2/ |
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103 |
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104 |
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105 ]]></help> |
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106 <expand macro="citations"> |
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107 <citation type="doi">10.1186/1471-2105-13-48</citation> |
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108 </expand> |
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109 |
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110 </tool> |