changeset 4:306a40d4bb8f draft

"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dada2 commit 52d835f027b052a0a887be14a55faf9fa9e456ae"
author iuc
date Mon, 01 Feb 2021 20:22:27 +0000
parents 0b884b080bb6
children 1d75bd0595e2
files macros.xml test-data/assignTaxonomyAddspecies.tab test-data/assignTaxonomyAddspecies_boot.tab tools.yml topics/metagenomics/metadata.yaml topics/metagenomics/tutorials/dada2/tutorial.bib topics/metagenomics/tutorials/dada2/tutorial.md topics/metagenomics/tutorials/dada2/workflows/main_workflow.ga
diffstat 8 files changed, 1540 insertions(+), 37 deletions(-) [+]
line wrap: on
line diff
--- a/macros.xml	Tue Jul 14 07:41:00 2020 -0400
+++ b/macros.xml	Mon Feb 01 20:22:27 2021 +0000
@@ -7,7 +7,7 @@
         </requirements>
     </xml>
 
-    <token name="@DADA2_VERSION@">1.16</token>
+    <token name="@DADA2_VERSION@">1.18</token>
     <token name="@WRAPPER_VERSION@">0</token>
 
     <xml name="version_command">
--- a/test-data/assignTaxonomyAddspecies.tab	Tue Jul 14 07:41:00 2020 -0400
+++ b/test-data/assignTaxonomyAddspecies.tab	Mon Feb 01 20:22:27 2021 +0000
@@ -1,7 +1,7 @@
 	Level1	Level2	Level3	Level4	Level5	NA	Species
 TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGCCTGCCAAGTCAGCGGTAAAATTGCGGGGCTCAACCCCGTACAGCCGTTGAAACTGCCGGGCTCGAGTGGGCGAGAAGTATGCGGAATGCGTGGTGTAGCGGTGAAATGCATAGATATCACGCAGAACCCCGATTGCGAAGGCAGCATACCGGCGCCCTACTGACGCTGAGGCACGAAAGTGCGGGGATCAAACAGG	Bacteria	Bacteroidetes	Cytophagia	Cytophagales	NA	NA	NA
-TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGGCTTTTAAGTCAGCGGTAAAAATTCGGGGCTCAACCCCGTCCGGCCGTTGAAACTGGGGGCCTTGAGTGGGCGAGAAGAAGGCGGAATGCGTGGTGTAGCGGTGAAATGCATAGATATCACGCAGAACCCCGATTGCGAAGGCAGCCTTCCGGCGCCCTACTGACGCTGAGGCACGAAAGTGCGGGGATCGAACAGG	Bacteria	Bacteroidetes	Cytophagia	Cytophagales	Cytophagaceae	NA	NA
-TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGCAGGCGGACTCTCAAGTCAGCGGTCAAATCGCGGGGCTCAACCCCGTTCCGCCGTTGAAACTGGGAGCCTTGAGTGCGCGAGAAGTAGGCGGAATGCGTGGTGTAGCGGTGAAATGCATAGATATCACGCAGAACTCCGATTGCGAAGGCAGCCTACCGGCGCGCAACTGACGCTCATGCACGAAAGCGTGGGTATCGAACAGG	Bacteria	Bacteroidetes	NA	NA	NA	NA	NA
+TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGGCTTTTAAGTCAGCGGTAAAAATTCGGGGCTCAACCCCGTCCGGCCGTTGAAACTGGGGGCCTTGAGTGGGCGAGAAGAAGGCGGAATGCGTGGTGTAGCGGTGAAATGCATAGATATCACGCAGAACCCCGATTGCGAAGGCAGCCTTCCGGCGCCCTACTGACGCTGAGGCACGAAAGTGCGGGGATCGAACAGG	Bacteria	Bacteroidetes	Cytophagia	Cytophagales	Cytophagaceae	Sporocytophaga	NA
+TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGCAGGCGGACTCTCAAGTCAGCGGTCAAATCGCGGGGCTCAACCCCGTTCCGCCGTTGAAACTGGGAGCCTTGAGTGCGCGAGAAGTAGGCGGAATGCGTGGTGTAGCGGTGAAATGCATAGATATCACGCAGAACTCCGATTGCGAAGGCAGCCTACCGGCGCGCAACTGACGCTCATGCACGAAAGCGTGGGTATCGAACAGG	Bacteria	Bacteroidetes	Cytophagia	Cytophagales	NA	NA	NA
 TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTCGATAAGTTAGAGGTGAAATCCCGGGGCTCAACTCCGGCACTGCCTCTGATACTGTCGGGCTAGAGTTTAGTTGCGGTAGGCGGAATGTATGGTGTAGCGGTGAAATGCATAGAGATCATACAGAACACCGATTGCGAAGGCAGCTTACCAAACTACGACTGACGTTGAGGCACGAAAGCGTGGGGAGCAAACAGG	Bacteria	Bacteroidetes	Cytophagia	Cytophagales	NA	NA	NA
 TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGGATGTCAAGTCAGCGGTAAAATTGTGGAGCTCAACTCCATCGAGCCGTTGAAACTGACGTTCTTGAGTGGGCGAGAAGTATGCGGAATGCGTGGTGTAGCGGTGAAATGCATAGATATCACGCAGAACTCCGATTGCGAAGGCAGCATACCGGCGCCCTACTGACGCTGAAGCACGAAAGCGTGGGTATCGAACAGG	Bacteria	Bacteroidetes	Cytophagia	Cytophagales	NA	NA	NA
 TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGAGCGTAGACGGTAATGCAAGTCTGGAGTGAAAGGCGGGGGCCCAACCCCCGGACTGCTCTGGAAACTGTGTAACTGGAGTGCAGGAGAGGCAGGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCCTGCTGGACTGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG	Bacteria	Firmicutes	Clostridia	Clostridiales	Lachnospiraceae	NA	NA
@@ -14,19 +14,19 @@
 TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGCATGGCAAGTCAGATGTGAAAGCCCGGGGCTCAACCCCGGGACTGCATTTGAAACTGCCAGGCTAGAGTGTCGGAGAGGCAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTGCTGGACGATCACTGACGCTGAGGCTCGAAAGCGTGGGGAGCAAACAGG	Bacteria	Firmicutes	Clostridia	Clostridiales	Lachnospiraceae	NA	NA
 TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCAGTGCAAGTCTGAAGTGAAAGGCGGGGGCCCAACCCCCGGACTGCTTTGGAAACTGTGCTGCTGGAGTGCAGGAGAGGCAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTGCTGGACTGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG	Bacteria	Firmicutes	Clostridia	Clostridiales	Lachnospiraceae	NA	NA
 TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGGCGGCCATGCAAGTCAGAAGTGAAAACCCGGGGCTCAACCCTGGGAGTGCTTTTGAAACTGTGCGGCTAGAGTGTCGGAGGGGTAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACGATGACTGACGCTGAGGCTCGAAAGCGTGGGGAGCAAACAGG	Bacteria	Firmicutes	Clostridia	Clostridiales	Lachnospiraceae	NA	NA
-TACGTAGGTGGCGAGCGTTGTCCGGATTTACTGGGCGTAAAGGGAGCGTAGGCGGACTTTTAAGTGAGATGTGAAATACTCGGGCTCAACTTGAGTGCTGCATTTCAAACTGGAAGTCTAGAGTGCAGGAGAGGAGAATGGAATTCCTAGTGTAGCGGTGAAATGCGTAGAGATTAGGAAGAACACCAGTGGCGAAGGCGATTCTCTGGACTGTAACTGACGCTGAGGCTCGAAAGCGTGGGGAGCAAACAGG	Bacteria	Firmicutes	NA	NA	NA	NA	NA
+TACGTAGGTGGCGAGCGTTGTCCGGATTTACTGGGCGTAAAGGGAGCGTAGGCGGACTTTTAAGTGAGATGTGAAATACTCGGGCTCAACTTGAGTGCTGCATTTCAAACTGGAAGTCTAGAGTGCAGGAGAGGAGAATGGAATTCCTAGTGTAGCGGTGAAATGCGTAGAGATTAGGAAGAACACCAGTGGCGAAGGCGATTCTCTGGACTGTAACTGACGCTGAGGCTCGAAAGCGTGGGGAGCAAACAGG	Bacteria	Firmicutes	Clostridia	Clostridiales	NA	NA	NA
 TACGTATGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGGCGGCCTGGCAAGCCTGATGTGAAATACCGGGGCCCAACCCCGGGGCTGCATTGGGAACTGCCAGGCTGGAGTGCCGGAGAGGCAGGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCCTGCTGGACGGTGACTGACGCTGAGGCTCGAAAGCGTGGGGAGCAAACAGG	Bacteria	Firmicutes	Clostridia	Clostridiales	Lachnospiraceae	NA	NA
 TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTTTTGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGGACTGCATTGGAAACTGTATGACTGGAGTGCAGGAGAGGTAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACTGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG	Bacteria	Firmicutes	Clostridia	Clostridiales	Lachnospiraceae	NA	NA
 TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTACGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGCCGGACTGGAGTGTCGGAGGGGTAAGCGGAATTCCTGGTGTAGCGGTGAAATGCGTAGATATCAGGAGGAACACCGGTGGCGAAGGCGGCTTACTGGACGATGACTGACGCTGAGGCTCGAAAGCGTGGGGAGCAAACAGG	Bacteria	Firmicutes	Clostridia	Clostridiales	Lachnospiraceae	NA	NA
-TACGTAGGGAGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGTAGGCGGCTTTGCAAGTCAGAAGTGAAATCCATGGGCTTAACCCATGAACTGCTTTTGAAACTGCAGAGCTTGAGTGGAGTAGAGGTAGGCGGAATTCCCGGTGTAGCGGTGAAATGCGTAGAGATCGGGAGGAACACCAGTGGCGAAGGCGGCCTGCTGGGCTCTAACTGACGCTGAGGCACGAAAGCGTGGGTAGCAAACAGG	Bacteria	NA	NA	NA	NA	NA	NA
+TACGTAGGGAGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGTAGGCGGCTTTGCAAGTCAGAAGTGAAATCCATGGGCTTAACCCATGAACTGCTTTTGAAACTGCAGAGCTTGAGTGGAGTAGAGGTAGGCGGAATTCCCGGTGTAGCGGTGAAATGCGTAGAGATCGGGAGGAACACCAGTGGCGAAGGCGGCCTGCTGGGCTCTAACTGACGCTGAGGCACGAAAGCGTGGGTAGCAAACAGG	Bacteria	Firmicutes	Clostridia	Clostridiales	NA	NA	NA
 TACGTAGGGAGCGAGCGTTATCCGGAATTATTGGGCGTAAAGGGTGCGTAGATGGCATAGTAAGTCTTTTGTAAAAATGCTGGGCTCAACCCAGTAGGGCAAAAGATACTGCAAAGCTAGAGTATGACAGAGGCAAGTGGAACTACATGTGTAGCGGTAAAATGCGTAAATATATGTAAGAACACCAGTGGCGAAGGCGGCTTGCTGGGTCGATACTGACATTGAGGCACGAAAGCGTGGGGAGCAAACAGG	Bacteria	NA	NA	NA	NA	NA	NA
 TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGGGACGCAAGTCAGATGTGAAAACCACGGGCTCAACCTGTGGCCTGCATTTGAAACTGTGTTTCTTGAGTACTGGAGAGGCAGACGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGTCTGCTGGACAGCAACTGACGCTGAGGCGCGAAAGCGTGGGGAGCAAACAGG	Bacteria	Firmicutes	Clostridia	Clostridiales	Ruminococcaceae	Oscillibacter	NA
 TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTGCGCCAAGTCTGGAGTGAAATGCCGCAGCTTAACTGCGGAACTGCTTTGGAAACTGGCGGACTAGAGTGCGGGAGGGGTAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCGGTGGCGAAGGCGGCTTACTGGACCGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG	Bacteria	Firmicutes	Clostridia	Clostridiales	Lachnospiraceae	Lachnoanaerobaculum	NA
-TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTGCTTAGGTCTGATGTGAAAGCCTTCGGCTTAACCGAAGAAGTGCATCGGAAACCGGGCGACTTGAGTGCAGAAGAGGACAGTGGAACTCCATGTGTAGCGGTGGAATGCGTAGATATATGGAAGAACACCAGTGGCGAAGGCGGCTGTCTGGTCTGCAACTGACGCTGAGGCTCGAAAGCATGGGTAGCGAACAGG	Bacteria	Firmicutes	Bacilli	Bacillales	NA	NA	NA
+TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTGCTTAGGTCTGATGTGAAAGCCTTCGGCTTAACCGAAGAAGTGCATCGGAAACCGGGCGACTTGAGTGCAGAAGAGGACAGTGGAACTCCATGTGTAGCGGTGGAATGCGTAGATATATGGAAGAACACCAGTGGCGAAGGCGGCTGTCTGGTCTGCAACTGACGCTGAGGCTCGAAAGCATGGGTAGCGAACAGG	Bacteria	Firmicutes	NA	NA	NA	NA	NA
 TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCAATGCAAGCCAGATGTGAAAACCCGCAGCTCAACTGGGGGAGTGCATTTGGAACTGTGTAGCTGGAGTGCAGGAGAGGTAAGCGGAATTCCTGGTGTAGCGGTGAAATGCGTAGATATCAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACTGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG	Bacteria	Firmicutes	Clostridia	Clostridiales	Lachnospiraceae	NA	NA
 TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGAGAGTAGGCGGCAAGGTAAGCGATATGTGAAAGCCTTAGGCTTAACCAAAGGATTGCATAACGAACTATCTAGCTAGAGTACAGGAGAGGAAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAAGAACACCAGTGGCGAAGGCGGCTTTCTGGACTGAAACTGACGCTGAGGCTCGAAAGCGTGGGGAGCGAACAGG	Bacteria	Firmicutes	NA	NA	NA	NA	NA
 TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGACGGCAGTGCAAGTCTGGAGTGAAAGCCCGGGGCCCAACCCCGGAACTGCTCTGGAAACTGTGCGGCTAGAGTACTGGAGGGGCAGGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCCTGCTGGACAGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG	Bacteria	Firmicutes	Clostridia	Clostridiales	Lachnospiraceae	NA	NA
 TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGGGAACGCAGGCGGTCTTTTAAGTCTGATGTGAAAGCCTTCGGCTTAACCGGAGTAGTGCATTGGAAACTGGGAGACTTGAGTGCAGAAGAGGAGAGTGGAACTCCATGTGTAGCGGTGAAATGCGTAGATATATGGAAGAACACCAGTGGCGAAAGCGGCTCTCTGGTCTGTAACTGACGCTGAGGTTCGAAAGCGTGGGTAGCAAACAGG	Bacteria	Firmicutes	Bacilli	Bacillales	Bacillaceae_2	Pullulanibacillus	NA
 TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCCAGACAAGTCTGAAGTGAAAATCCAGCGCTTAACGTTGGAAGTGCTTTGGAAACTGCCGGGCTAGAGTGCAGGAGGGGCAGGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCCTGCTGGACTGCAACTGACGTTGAGGCTCGAAGGCGTGGGGAGCAAACAGG	Bacteria	Firmicutes	Clostridia	Clostridiales	Lachnospiraceae	Lachnoanaerobaculum	NA
 TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGTGTAGCCGGGTTGACAAGTCAGATGTGAAATCCTGCGGCTTAACCGCAGAACTGCATTTGAAACTGTTGATCTTGAGTACTGGAGAGGCAGACGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGTCTGCTGGACAGCAACTGACGGTGAGGCGCGAAAGCGTGGGGAGCAAACAGG	Bacteria	Firmicutes	Clostridia	Clostridiales	Ruminococcaceae	Oscillibacter	NA
-TACGTAGGGAGCAAGCGTTGTTCGGAATGACTGGGCGTAAAGGGTGCGTAGGCGGTTGTACAAGTTAGAAGTGAAATACCCAGGGCTTAACTCGGGTGCTGCTTCTAAAACTGTATGACTTGAGTGCAGTAGAGGTTAGTGGAATTCCTAGTGTAGCGGTGGAATGCGTAGATATTAGGAGGAACACCAGAGGCGAAGGCGGCTAACTGGACTGCAACTGACGCTGAGGCACGAAAGTGTGGGGAGCAAACAGG	Bacteria	Firmicutes	NA	NA	NA	NA	NA
+TACGTAGGGAGCAAGCGTTGTTCGGAATGACTGGGCGTAAAGGGTGCGTAGGCGGTTGTACAAGTTAGAAGTGAAATACCCAGGGCTTAACTCGGGTGCTGCTTCTAAAACTGTATGACTTGAGTGCAGTAGAGGTTAGTGGAATTCCTAGTGTAGCGGTGGAATGCGTAGATATTAGGAGGAACACCAGAGGCGAAGGCGGCTAACTGGACTGCAACTGACGCTGAGGCACGAAAGTGTGGGGAGCAAACAGG	Bacteria	NA	NA	NA	NA	NA	NA
--- a/test-data/assignTaxonomyAddspecies_boot.tab	Tue Jul 14 07:41:00 2020 -0400
+++ b/test-data/assignTaxonomyAddspecies_boot.tab	Mon Feb 01 20:22:27 2021 +0000
@@ -1,32 +1,32 @@
 	Level1	Level2	Level3	Level4	Level5	NA
-TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGCCTGCCAAGTCAGCGGTAAAATTGCGGGGCTCAACCCCGTACAGCCGTTGAAACTGCCGGGCTCGAGTGGGCGAGAAGTATGCGGAATGCGTGGTGTAGCGGTGAAATGCATAGATATCACGCAGAACCCCGATTGCGAAGGCAGCATACCGGCGCCCTACTGACGCTGAGGCACGAAAGTGCGGGGATCAAACAGG	98	80	70	70	48	48
-TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGGCTTTTAAGTCAGCGGTAAAAATTCGGGGCTCAACCCCGTCCGGCCGTTGAAACTGGGGGCCTTGAGTGGGCGAGAAGAAGGCGGAATGCGTGGTGTAGCGGTGAAATGCATAGATATCACGCAGAACCCCGATTGCGAAGGCAGCCTTCCGGCGCCCTACTGACGCTGAGGCACGAAAGTGCGGGGATCGAACAGG	97	88	76	76	50	48
-TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGCAGGCGGACTCTCAAGTCAGCGGTCAAATCGCGGGGCTCAACCCCGTTCCGCCGTTGAAACTGGGAGCCTTGAGTGCGCGAGAAGTAGGCGGAATGCGTGGTGTAGCGGTGAAATGCATAGATATCACGCAGAACTCCGATTGCGAAGGCAGCCTACCGGCGCGCAACTGACGCTCATGCACGAAAGCGTGGGTATCGAACAGG	100	82	49	49	42	42
-TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTCGATAAGTTAGAGGTGAAATCCCGGGGCTCAACTCCGGCACTGCCTCTGATACTGTCGGGCTAGAGTTTAGTTGCGGTAGGCGGAATGTATGGTGTAGCGGTGAAATGCATAGAGATCATACAGAACACCGATTGCGAAGGCAGCTTACCAAACTACGACTGACGTTGAGGCACGAAAGCGTGGGGAGCAAACAGG	100	76	73	73	46	46
-TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGGATGTCAAGTCAGCGGTAAAATTGTGGAGCTCAACTCCATCGAGCCGTTGAAACTGACGTTCTTGAGTGGGCGAGAAGTATGCGGAATGCGTGGTGTAGCGGTGAAATGCATAGATATCACGCAGAACTCCGATTGCGAAGGCAGCATACCGGCGCCCTACTGACGCTGAAGCACGAAAGCGTGGGTATCGAACAGG	97	82	68	68	33	33
-TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGAGCGTAGACGGTAATGCAAGTCTGGAGTGAAAGGCGGGGGCCCAACCCCCGGACTGCTCTGGAAACTGTGTAACTGGAGTGCAGGAGAGGCAGGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCCTGCTGGACTGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG	100	100	99	99	99	91
-TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTGGGCGGAATTCGTGGTGTAGCGGTGAAATGCTTAGATATCACGAAGAACTCCGATTGCGAAGGCAGCTCACTGGACTGCAACTGACACTGATGCTCGAAAGTGTGGGTATCAAACAGG	96	45	31	31	13	13
-TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGGATGCCAAGTCAGCGGTAAAAAAGCGGTGCTCAACGCCGTCGAGCCGTTGAAACTGGCGTTCTTGAGTGGGCGAGAAGTATGCGGAATGCGTGGTGTAGCGGTGAAATGCATAGATATCACGCAGAACTCCGATTGCGAAGGCAGCATACCGGCGCCCTACTGACGCTGAGGCACGAAAGCGTGGGTATCGAACAGG	99	92	80	80	49	49
-TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGAGCGCGCAGGTGGTTAATTAAGTCTGATGTGAAAGCCCACGGCTTAACCGTGGAGGGTCATTGGAAACTGGTTGACTTGAGTGCAGAAGAGGGAAGTGGAATTCCATGTGTAGCGGTGAAATGCGTAGAGATATGGAGGAACACCAGTGGCGAAGGCGGCTTCCTGGTCTGCAACTGACACTGAGGCGCGAAAGCGTGGGGAGCAAACAGG	100	100	100	100	72	72
-TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTGAGACAAGTCTGAAGTGAAAGCCCGGGGCTCAACCCCGGGACTGCTTTGGAAACTGCCTGACTGGAGTGCTGGAGAGGCAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAAGAACACCAGTGGCGAAGGCGGCTTGCTGGACAGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG	100	100	100	100	100	43
-TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGTGCAGGCGGTTCAATAAGTCTGATGTGAAAGCCTTCGGCTCAACCGGAGAATTGCATCAGAAACTGTTGAACTTGAGTGCAGAAGAGGAGAGTGGAACTCCATGTGTAGCGGTGGAATGCGTAGATATATGGAAGAACACCAGTGGCGAAGGCGGCTCTCTGGTCTGCAACTGACGCTGAGGCTCGAAAGCATGGGTAGCGAACAGG	100	94	87	87	68	41
-TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCAGCGCAAGTCTGGAGTGAAATGCCGGGGCCCAACCCCGGAACTGCTTTGGAAACTGTGCAGCTCGAGTGCAGGAGAGGTAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACTGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG	100	100	100	100	100	57
+TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGCCTGCCAAGTCAGCGGTAAAATTGCGGGGCTCAACCCCGTACAGCCGTTGAAACTGCCGGGCTCGAGTGGGCGAGAAGTATGCGGAATGCGTGGTGTAGCGGTGAAATGCATAGATATCACGCAGAACCCCGATTGCGAAGGCAGCATACCGGCGCCCTACTGACGCTGAGGCACGAAAGTGCGGGGATCAAACAGG	99	82	68	68	42	42
+TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGGCTTTTAAGTCAGCGGTAAAAATTCGGGGCTCAACCCCGTCCGGCCGTTGAAACTGGGGGCCTTGAGTGGGCGAGAAGAAGGCGGAATGCGTGGTGTAGCGGTGAAATGCATAGATATCACGCAGAACCCCGATTGCGAAGGCAGCCTTCCGGCGCCCTACTGACGCTGAGGCACGAAAGTGCGGGGATCGAACAGG	99	89	73	73	54	54
+TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGCAGGCGGACTCTCAAGTCAGCGGTCAAATCGCGGGGCTCAACCCCGTTCCGCCGTTGAAACTGGGAGCCTTGAGTGCGCGAGAAGTAGGCGGAATGCGTGGTGTAGCGGTGAAATGCATAGATATCACGCAGAACTCCGATTGCGAAGGCAGCCTACCGGCGCGCAACTGACGCTCATGCACGAAAGCGTGGGTATCGAACAGG	97	81	52	52	38	38
+TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTCGATAAGTTAGAGGTGAAATCCCGGGGCTCAACTCCGGCACTGCCTCTGATACTGTCGGGCTAGAGTTTAGTTGCGGTAGGCGGAATGTATGGTGTAGCGGTGAAATGCATAGAGATCATACAGAACACCGATTGCGAAGGCAGCTTACCAAACTACGACTGACGTTGAGGCACGAAAGCGTGGGGAGCAAACAGG	100	74	74	74	42	42
+TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGGATGTCAAGTCAGCGGTAAAATTGTGGAGCTCAACTCCATCGAGCCGTTGAAACTGACGTTCTTGAGTGGGCGAGAAGTATGCGGAATGCGTGGTGTAGCGGTGAAATGCATAGATATCACGCAGAACTCCGATTGCGAAGGCAGCATACCGGCGCCCTACTGACGCTGAAGCACGAAAGCGTGGGTATCGAACAGG	94	74	65	65	39	39
+TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGAGCGTAGACGGTAATGCAAGTCTGGAGTGAAAGGCGGGGGCCCAACCCCCGGACTGCTCTGGAAACTGTGTAACTGGAGTGCAGGAGAGGCAGGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCCTGCTGGACTGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG	100	100	100	100	100	84
+TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTGGGCGGAATTCGTGGTGTAGCGGTGAAATGCTTAGATATCACGAAGAACTCCGATTGCGAAGGCAGCTCACTGGACTGCAACTGACACTGATGCTCGAAAGTGTGGGTATCAAACAGG	95	41	17	17	13	13
+TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGGATGCCAAGTCAGCGGTAAAAAAGCGGTGCTCAACGCCGTCGAGCCGTTGAAACTGGCGTTCTTGAGTGGGCGAGAAGTATGCGGAATGCGTGGTGTAGCGGTGAAATGCATAGATATCACGCAGAACTCCGATTGCGAAGGCAGCATACCGGCGCCCTACTGACGCTGAGGCACGAAAGCGTGGGTATCGAACAGG	93	74	63	63	41	41
+TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGAGCGCGCAGGTGGTTAATTAAGTCTGATGTGAAAGCCCACGGCTTAACCGTGGAGGGTCATTGGAAACTGGTTGACTTGAGTGCAGAAGAGGGAAGTGGAATTCCATGTGTAGCGGTGAAATGCGTAGAGATATGGAGGAACACCAGTGGCGAAGGCGGCTTCCTGGTCTGCAACTGACACTGAGGCGCGAAAGCGTGGGGAGCAAACAGG	100	100	99	99	61	61
+TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTGAGACAAGTCTGAAGTGAAAGCCCGGGGCTCAACCCCGGGACTGCTTTGGAAACTGCCTGACTGGAGTGCTGGAGAGGCAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAAGAACACCAGTGGCGAAGGCGGCTTGCTGGACAGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG	100	100	100	100	100	39
+TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGTGCAGGCGGTTCAATAAGTCTGATGTGAAAGCCTTCGGCTCAACCGGAGAATTGCATCAGAAACTGTTGAACTTGAGTGCAGAAGAGGAGAGTGGAACTCCATGTGTAGCGGTGGAATGCGTAGATATATGGAAGAACACCAGTGGCGAAGGCGGCTCTCTGGTCTGCAACTGACGCTGAGGCTCGAAAGCATGGGTAGCGAACAGG	100	90	83	83	64	30
+TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCAGCGCAAGTCTGGAGTGAAATGCCGGGGCCCAACCCCGGAACTGCTTTGGAAACTGTGCAGCTCGAGTGCAGGAGAGGTAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACTGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG	100	100	100	100	100	62
 TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGCATGGCAAGTCAGATGTGAAAGCCCGGGGCTCAACCCCGGGACTGCATTTGAAACTGCCAGGCTAGAGTGTCGGAGAGGCAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTGCTGGACGATCACTGACGCTGAGGCTCGAAAGCGTGGGGAGCAAACAGG	100	100	100	100	100	96
-TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCAGTGCAAGTCTGAAGTGAAAGGCGGGGGCCCAACCCCCGGACTGCTTTGGAAACTGTGCTGCTGGAGTGCAGGAGAGGCAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTGCTGGACTGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG	100	100	100	100	100	64
-TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGGCGGCCATGCAAGTCAGAAGTGAAAACCCGGGGCTCAACCCTGGGAGTGCTTTTGAAACTGTGCGGCTAGAGTGTCGGAGGGGTAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACGATGACTGACGCTGAGGCTCGAAAGCGTGGGGAGCAAACAGG	100	100	100	100	100	88
-TACGTAGGTGGCGAGCGTTGTCCGGATTTACTGGGCGTAAAGGGAGCGTAGGCGGACTTTTAAGTGAGATGTGAAATACTCGGGCTCAACTTGAGTGCTGCATTTCAAACTGGAAGTCTAGAGTGCAGGAGAGGAGAATGGAATTCCTAGTGTAGCGGTGAAATGCGTAGAGATTAGGAAGAACACCAGTGGCGAAGGCGATTCTCTGGACTGTAACTGACGCTGAGGCTCGAAAGCGTGGGGAGCAAACAGG	100	79	38	38	33	26
-TACGTATGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGGCGGCCTGGCAAGCCTGATGTGAAATACCGGGGCCCAACCCCGGGGCTGCATTGGGAACTGCCAGGCTGGAGTGCCGGAGAGGCAGGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCCTGCTGGACGGTGACTGACGCTGAGGCTCGAAAGCGTGGGGAGCAAACAGG	100	100	100	100	100	97
-TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTTTTGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGGACTGCATTGGAAACTGTATGACTGGAGTGCAGGAGAGGTAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACTGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG	100	100	100	100	100	70
-TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTACGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGCCGGACTGGAGTGTCGGAGGGGTAAGCGGAATTCCTGGTGTAGCGGTGAAATGCGTAGATATCAGGAGGAACACCGGTGGCGAAGGCGGCTTACTGGACGATGACTGACGCTGAGGCTCGAAAGCGTGGGGAGCAAACAGG	100	100	99	99	99	81
-TACGTAGGGAGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGTAGGCGGCTTTGCAAGTCAGAAGTGAAATCCATGGGCTTAACCCATGAACTGCTTTTGAAACTGCAGAGCTTGAGTGGAGTAGAGGTAGGCGGAATTCCCGGTGTAGCGGTGAAATGCGTAGAGATCGGGAGGAACACCAGTGGCGAAGGCGGCCTGCTGGGCTCTAACTGACGCTGAGGCACGAAAGCGTGGGTAGCAAACAGG	100	49	46	46	43	30
-TACGTAGGGAGCGAGCGTTATCCGGAATTATTGGGCGTAAAGGGTGCGTAGATGGCATAGTAAGTCTTTTGTAAAAATGCTGGGCTCAACCCAGTAGGGCAAAAGATACTGCAAAGCTAGAGTATGACAGAGGCAAGTGGAACTACATGTGTAGCGGTAAAATGCGTAAATATATGTAAGAACACCAGTGGCGAAGGCGGCTTGCTGGGTCGATACTGACATTGAGGCACGAAAGCGTGGGGAGCAAACAGG	97	33	27	17	15	15
-TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGGGACGCAAGTCAGATGTGAAAACCACGGGCTCAACCTGTGGCCTGCATTTGAAACTGTGTTTCTTGAGTACTGGAGAGGCAGACGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGTCTGCTGGACAGCAACTGACGCTGAGGCGCGAAAGCGTGGGGAGCAAACAGG	100	99	98	98	70	70
-TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTGCGCCAAGTCTGGAGTGAAATGCCGCAGCTTAACTGCGGAACTGCTTTGGAAACTGGCGGACTAGAGTGCGGGAGGGGTAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCGGTGGCGAAGGCGGCTTACTGGACCGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG	100	100	100	100	100	50
-TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTGCTTAGGTCTGATGTGAAAGCCTTCGGCTTAACCGAAGAAGTGCATCGGAAACCGGGCGACTTGAGTGCAGAAGAGGACAGTGGAACTCCATGTGTAGCGGTGGAATGCGTAGATATATGGAAGAACACCAGTGGCGAAGGCGGCTGTCTGGTCTGCAACTGACGCTGAGGCTCGAAAGCATGGGTAGCGAACAGG	100	66	57	57	35	35
-TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCAATGCAAGCCAGATGTGAAAACCCGCAGCTCAACTGGGGGAGTGCATTTGGAACTGTGTAGCTGGAGTGCAGGAGAGGTAAGCGGAATTCCTGGTGTAGCGGTGAAATGCGTAGATATCAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACTGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG	100	100	100	100	99	75
-TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGAGAGTAGGCGGCAAGGTAAGCGATATGTGAAAGCCTTAGGCTTAACCAAAGGATTGCATAACGAACTATCTAGCTAGAGTACAGGAGAGGAAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAAGAACACCAGTGGCGAAGGCGGCTTTCTGGACTGAAACTGACGCTGAGGCTCGAAAGCGTGGGGAGCGAACAGG	100	96	42	42	42	42
-TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGACGGCAGTGCAAGTCTGGAGTGAAAGCCCGGGGCCCAACCCCGGAACTGCTCTGGAAACTGTGCGGCTAGAGTACTGGAGGGGCAGGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCCTGCTGGACAGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG	100	100	100	100	100	41
-TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGGGAACGCAGGCGGTCTTTTAAGTCTGATGTGAAAGCCTTCGGCTTAACCGGAGTAGTGCATTGGAAACTGGGAGACTTGAGTGCAGAAGAGGAGAGTGGAACTCCATGTGTAGCGGTGAAATGCGTAGATATATGGAAGAACACCAGTGGCGAAAGCGGCTCTCTGGTCTGTAACTGACGCTGAGGTTCGAAAGCGTGGGTAGCAAACAGG	100	100	98	98	87	52
-TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCCAGACAAGTCTGAAGTGAAAATCCAGCGCTTAACGTTGGAAGTGCTTTGGAAACTGCCGGGCTAGAGTGCAGGAGGGGCAGGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCCTGCTGGACTGCAACTGACGTTGAGGCTCGAAGGCGTGGGGAGCAAACAGG	100	100	100	100	100	62
-TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGTGTAGCCGGGTTGACAAGTCAGATGTGAAATCCTGCGGCTTAACCGCAGAACTGCATTTGAAACTGTTGATCTTGAGTACTGGAGAGGCAGACGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGTCTGCTGGACAGCAACTGACGGTGAGGCGCGAAAGCGTGGGGAGCAAACAGG	100	98	98	98	89	89
-TACGTAGGGAGCAAGCGTTGTTCGGAATGACTGGGCGTAAAGGGTGCGTAGGCGGTTGTACAAGTTAGAAGTGAAATACCCAGGGCTTAACTCGGGTGCTGCTTCTAAAACTGTATGACTTGAGTGCAGTAGAGGTTAGTGGAATTCCTAGTGTAGCGGTGGAATGCGTAGATATTAGGAGGAACACCAGAGGCGAAGGCGGCTAACTGGACTGCAACTGACGCTGAGGCACGAAAGTGTGGGGAGCAAACAGG	100	53	38	38	38	26
+TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCAGTGCAAGTCTGAAGTGAAAGGCGGGGGCCCAACCCCCGGACTGCTTTGGAAACTGTGCTGCTGGAGTGCAGGAGAGGCAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTGCTGGACTGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG	100	100	100	100	100	58
+TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGGCGGCCATGCAAGTCAGAAGTGAAAACCCGGGGCTCAACCCTGGGAGTGCTTTTGAAACTGTGCGGCTAGAGTGTCGGAGGGGTAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACGATGACTGACGCTGAGGCTCGAAAGCGTGGGGAGCAAACAGG	100	100	100	100	100	92
+TACGTAGGTGGCGAGCGTTGTCCGGATTTACTGGGCGTAAAGGGAGCGTAGGCGGACTTTTAAGTGAGATGTGAAATACTCGGGCTCAACTTGAGTGCTGCATTTCAAACTGGAAGTCTAGAGTGCAGGAGAGGAGAATGGAATTCCTAGTGTAGCGGTGAAATGCGTAGAGATTAGGAAGAACACCAGTGGCGAAGGCGATTCTCTGGACTGTAACTGACGCTGAGGCTCGAAAGCGTGGGGAGCAAACAGG	100	79	50	50	40	32
+TACGTATGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGGCGGCCTGGCAAGCCTGATGTGAAATACCGGGGCCCAACCCCGGGGCTGCATTGGGAACTGCCAGGCTGGAGTGCCGGAGAGGCAGGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCCTGCTGGACGGTGACTGACGCTGAGGCTCGAAAGCGTGGGGAGCAAACAGG	100	100	100	100	100	95
+TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTTTTGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGGACTGCATTGGAAACTGTATGACTGGAGTGCAGGAGAGGTAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACTGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG	100	100	100	100	100	77
+TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTACGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGCCGGACTGGAGTGTCGGAGGGGTAAGCGGAATTCCTGGTGTAGCGGTGAAATGCGTAGATATCAGGAGGAACACCGGTGGCGAAGGCGGCTTACTGGACGATGACTGACGCTGAGGCTCGAAAGCGTGGGGAGCAAACAGG	100	100	100	100	100	79
+TACGTAGGGAGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGTAGGCGGCTTTGCAAGTCAGAAGTGAAATCCATGGGCTTAACCCATGAACTGCTTTTGAAACTGCAGAGCTTGAGTGGAGTAGAGGTAGGCGGAATTCCCGGTGTAGCGGTGAAATGCGTAGAGATCGGGAGGAACACCAGTGGCGAAGGCGGCCTGCTGGGCTCTAACTGACGCTGAGGCACGAAAGCGTGGGTAGCAAACAGG	100	61	60	60	48	39
+TACGTAGGGAGCGAGCGTTATCCGGAATTATTGGGCGTAAAGGGTGCGTAGATGGCATAGTAAGTCTTTTGTAAAAATGCTGGGCTCAACCCAGTAGGGCAAAAGATACTGCAAAGCTAGAGTATGACAGAGGCAAGTGGAACTACATGTGTAGCGGTAAAATGCGTAAATATATGTAAGAACACCAGTGGCGAAGGCGGCTTGCTGGGTCGATACTGACATTGAGGCACGAAAGCGTGGGGAGCAAACAGG	99	30	30	27	22	22
+TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGGGACGCAAGTCAGATGTGAAAACCACGGGCTCAACCTGTGGCCTGCATTTGAAACTGTGTTTCTTGAGTACTGGAGAGGCAGACGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGTCTGCTGGACAGCAACTGACGCTGAGGCGCGAAAGCGTGGGGAGCAAACAGG	100	96	94	94	73	73
+TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTGCGCCAAGTCTGGAGTGAAATGCCGCAGCTTAACTGCGGAACTGCTTTGGAAACTGGCGGACTAGAGTGCGGGAGGGGTAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCGGTGGCGAAGGCGGCTTACTGGACCGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG	100	100	100	100	100	51
+TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTGCTTAGGTCTGATGTGAAAGCCTTCGGCTTAACCGAAGAAGTGCATCGGAAACCGGGCGACTTGAGTGCAGAAGAGGACAGTGGAACTCCATGTGTAGCGGTGGAATGCGTAGATATATGGAAGAACACCAGTGGCGAAGGCGGCTGTCTGGTCTGCAACTGACGCTGAGGCTCGAAAGCATGGGTAGCGAACAGG	100	77	47	47	30	30
+TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCAATGCAAGCCAGATGTGAAAACCCGCAGCTCAACTGGGGGAGTGCATTTGGAACTGTGTAGCTGGAGTGCAGGAGAGGTAAGCGGAATTCCTGGTGTAGCGGTGAAATGCGTAGATATCAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACTGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG	100	99	99	99	99	80
+TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGAGAGTAGGCGGCAAGGTAAGCGATATGTGAAAGCCTTAGGCTTAACCAAAGGATTGCATAACGAACTATCTAGCTAGAGTACAGGAGAGGAAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAAGAACACCAGTGGCGAAGGCGGCTTTCTGGACTGAAACTGACGCTGAGGCTCGAAAGCGTGGGGAGCGAACAGG	100	96	45	45	45	45
+TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGACGGCAGTGCAAGTCTGGAGTGAAAGCCCGGGGCCCAACCCCGGAACTGCTCTGGAAACTGTGCGGCTAGAGTACTGGAGGGGCAGGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCCTGCTGGACAGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGG	100	100	100	100	100	34
+TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGGGAACGCAGGCGGTCTTTTAAGTCTGATGTGAAAGCCTTCGGCTTAACCGGAGTAGTGCATTGGAAACTGGGAGACTTGAGTGCAGAAGAGGAGAGTGGAACTCCATGTGTAGCGGTGAAATGCGTAGATATATGGAAGAACACCAGTGGCGAAAGCGGCTCTCTGGTCTGTAACTGACGCTGAGGTTCGAAAGCGTGGGTAGCAAACAGG	100	99	97	97	90	58
+TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCCAGACAAGTCTGAAGTGAAAATCCAGCGCTTAACGTTGGAAGTGCTTTGGAAACTGCCGGGCTAGAGTGCAGGAGGGGCAGGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCCTGCTGGACTGCAACTGACGTTGAGGCTCGAAGGCGTGGGGAGCAAACAGG	100	100	99	99	99	62
+TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGTGTAGCCGGGTTGACAAGTCAGATGTGAAATCCTGCGGCTTAACCGCAGAACTGCATTTGAAACTGTTGATCTTGAGTACTGGAGAGGCAGACGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGTCTGCTGGACAGCAACTGACGGTGAGGCGCGAAAGCGTGGGGAGCAAACAGG	100	99	99	99	95	95
+TACGTAGGGAGCAAGCGTTGTTCGGAATGACTGGGCGTAAAGGGTGCGTAGGCGGTTGTACAAGTTAGAAGTGAAATACCCAGGGCTTAACTCGGGTGCTGCTTCTAAAACTGTATGACTTGAGTGCAGTAGAGGTTAGTGGAATTCCTAGTGTAGCGGTGGAATGCGTAGATATTAGGAGGAACACCAGAGGCGAAGGCGGCTAACTGGACTGCAACTGACGCTGAGGCACGAAAGTGTGGGGAGCAAACAGG	100	42	31	31	31	22
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/tools.yml	Mon Feb 01 20:22:27 2021 +0000
@@ -0,0 +1,5 @@
+install_tool_dependencies: True
+install_repository_dependencies: True
+install_resolver_dependencies: True
+
+tools: []
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/topics/metagenomics/metadata.yaml	Mon Feb 01 20:22:27 2021 +0000
@@ -0,0 +1,28 @@
+---
+name: "metagenomics"
+type: "use"
+title: "Metagenomics"
+summary: "Metagenomics is a discipline that enables the genomic study of uncultured microorganisms"
+edam_ontology: "topic_3174"
+docker_image: "quay.io/galaxy/metagenomics-training"
+
+requirements:
+  -
+    type: "internal"
+    topic_name: introduction
+
+maintainers:
+  - bebatut
+  - shiltemann
+
+references:
+  -
+    authors: "Kozich JJ, Westcott SL, Baxter NT, Highlander SK, Schloss PD."
+    title: "Development of a dual-index sequencing strategy and curation pipeline for analyzing amplicon sequence data on the MiSeq Illumina sequencing platform. Applied and Environmental Microbiology"
+    link: "http://www.schlosslab.org/assets/pdf/2013_kozich.pdf"
+    summary: ""
+  -
+    authors: "Pablo Yarza, Pelin Yilmaz, Elmar Pruesse, Frank Oliver Glöckner, Wolfgang Ludwig, Karl-Heinz Schleifer, William B. Whitman, Jean Euzéby, Rudolf Amann, Ramon Rosselló-Móra"
+    title: "Uniting the classification of cultured and uncultured bacteria and archaea using 16S rRNA gene sequences."
+    link: "https://www.ncbi.nlm.nih.gov/pubmed/25118885"
+    summary: ""
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/topics/metagenomics/tutorials/dada2/tutorial.bib	Mon Feb 01 20:22:27 2021 +0000
@@ -0,0 +1,30 @@
+
+# This is the bibliography file for your tutorial.
+#
+# To add bibliography (bibtex) entries here, follow these steps:
+#  1) Find the DOI for the article you want to cite
+#  2) Go to https://doi2bib.org and fill in the DOI
+#  3) Copy the resulting bibtex entry into this file
+#
+# To cite the example below, in your tutorial.md file
+# use {% Batut2018 %}
+
+@article{Batut2018,
+  doi = {10.1016/j.cels.2018.05.012},
+  url = {https://doi.org/10.1016/j.cels.2018.05.012},
+  year = {2018},
+  month = jun,
+  publisher = {Elsevier {BV}},
+  volume = {6},
+  number = {6},
+  pages = {752--758.e1},
+  author = {B{\'{e}}r{\'{e}}nice Batut and Saskia Hiltemann and Andrea Bagnacani and Dannon Baker and Vivek Bhardwaj and
+           Clemens Blank and Anthony Bretaudeau and Loraine Brillet-Gu{\'{e}}guen and Martin {\v{C}}ech and John Chilton
+           and Dave Clements and Olivia Doppelt-Azeroual and Anika Erxleben and Mallory Ann Freeberg and Simon Gladman and
+           Youri Hoogstrate and Hans-Rudolf Hotz and Torsten Houwaart and Pratik Jagtap and Delphine Larivi{\`{e}}re and
+           Gildas Le Corguill{\'{e}} and Thomas Manke and Fabien Mareuil and Fidel Ram{\'{i}}rez and Devon Ryan and
+           Florian Christoph Sigloch and Nicola Soranzo and Joachim Wolff and Pavankumar Videm and Markus Wolfien and
+           Aisanjiang Wubuli and Dilmurat Yusuf and James Taylor and Rolf Backofen and Anton Nekrutenko and Bj\"{o}rn Gr\"{u}ning},
+  title = {Community-Driven Data Analysis Training for Biology},
+  journal = {Cell Systems}
+}
\ No newline at end of file
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/topics/metagenomics/tutorials/dada2/tutorial.md	Mon Feb 01 20:22:27 2021 +0000
@@ -0,0 +1,642 @@
+---
+layout: tutorial_hands_on
+
+title: 16S Microbial Analysis with mothur (extended)
+zenodo_link: https://doi.org/10.5281/zenodo.800651
+questions:
+- What is the effect of normal variation in the gut microbiome on host health?
+objectives:
+- Analyze of 16S rRNA sequencing data using the mothur toolsuite in Galaxy
+- Using a mock community to assess the error rate of your sequencing experiment
+- Visualize sample diversity using Krona and Phinch
+time_estimation: ''
+key_points:
+- 16S rRNA gene sequencing analysis results depend on the many algorithms used and
+  their settings
+- Quality control and cleaning of your data is a crucial step in order to obtain optimal
+  results
+- Adding a mock community to serve as a control sample can help you asses the error
+  rate of your experimental setup
+- We can explore alpha and beta diversities using Krona and Phinch for dynamic visualizations
+contributors:
+- shiltemann
+- bebatut
+- tnabtaf
+
+---
+
+
+# Introduction
+{:.no_toc}
+
+<!-- This is a comment. -->
+
+General introduction about the topic and then an introduction of the
+tutorial (the questions and the objectives). It is nice also to have a
+scheme to sum up the pipeline used during the tutorial. The idea is to
+give to trainees insight into the content of the tutorial and the (theoretical
+and technical) key concepts they will learn.
+
+You may want to cite some publications; this can be done by adding citations to the
+bibliography file (`tutorial.bib` file next to your `tutorial.md` file). These citations
+must be in bibtex format. If you have the DOI for the paper you wish to cite, you can
+get the corresponding bibtex entry using [doi2bib.org](https://doi2bib.org).
+
+With the example you will find in the `tutorial.bib` file, you can add a citation to
+this article here in your tutorial like this:
+{% raw %} `{% cite Batut2018 %}`{% endraw %}.
+This will be rendered like this: {% cite Batut2018 %}, and links to a
+[bibliography section](#bibliography) which will automatically be created at the end of the
+tutorial.
+
+
+**Please follow our
+[tutorial to learn how to fill the Markdown]({{ site.baseurl }}/topics/contributing/tutorials/create-new-tutorial-content/tutorial.html)**
+
+> ### Agenda
+>
+> In this tutorial, we will cover:
+>
+> 1. TOC
+> {:toc}
+>
+{: .agenda}
+
+# Title for your first section
+
+Give some background about what the trainees will be doing in the section.
+Remember that many people reading your materials will likely be novices,
+so make sure to explain all the relevant concepts.
+
+## Title for a subsection
+Section and subsection titles will be displayed in the tutorial index on the left side of
+the page, so try to make them informative and concise!
+
+# Hands-on Sections
+Below are a series of hand-on boxes, one for each tool in your workflow file.
+Often you may wish to combine several boxes into one or make other adjustments such
+as breaking the tutorial into sections, we encourage you to make such changes as you
+see fit, this is just a starting point :)
+
+Anywhere you find the word "***TODO***", there is something that needs to be changed
+depending on the specifics of your tutorial.
+
+have fun!
+
+## Get data
+
+> ### {% icon hands_on %} Hands-on: Data upload
+>
+> 1. Create a new history for this tutorial
+> 2. Import the files from [Zenodo]() or from the shared data library
+>
+>    ```
+>    
+>    ```
+>    ***TODO***: *Add the files by the ones on Zenodo here (if not added)*
+>
+>    ***TODO***: *Remove the useless files (if added)*
+>
+>    {% include snippets/import_via_link.md %}
+>    {% include snippets/import_from_data_library.md %}
+>
+> 3. Rename the datasets
+> 4. Check that the datatype
+>
+>    {% include snippets/change_datatype.md datatype="datatypes" %}
+>
+> 5. Add to each database a tag corresponding to ...
+>
+>    {% include snippets/add_tag.md %}
+>
+{: .hands_on}
+
+# Title of the section usually corresponding to a big step in the analysis
+
+It comes first a description of the step: some background and some theory.
+Some image can be added there to support the theory explanation:
+
+![Alternative text](../../images/image_name "Legend of the image")
+
+The idea is to keep the theory description before quite simple to focus more on the practical part.
+
+***TODO***: *Consider adding a detail box to expand the theory*
+
+> ### {% icon details %} More details about the theory
+>
+> But to describe more details, it is possible to use the detail boxes which are expandable
+>
+{: .details}
+
+A big step can have several subsections or sub steps:
+
+
+## Sub-step with **dada2_plotComplexity**
+
+> ### {% icon hands_on %} Hands-on: Task description
+>
+> 1. **dada2_plotComplexity** {% icon tool %} with the following parameters:
+>
+>    ***TODO***: *Check parameter descriptions*
+>
+>    ***TODO***: *Consider adding a comment or tip box*
+>
+>    > ### {% icon comment %} Comment
+>    >
+>    > A comment about the tool or something else. This box can also be in the main text
+>    {: .comment}
+>
+{: .hands_on}
+
+***TODO***: *Consider adding a question to test the learners understanding of the previous exercise*
+
+> ### {% icon question %} Questions
+>
+> 1. Question1?
+> 2. Question2?
+>
+> > ### {% icon solution %} Solution
+> >
+> > 1. Answer for question1
+> > 2. Answer for question2
+> >
+> {: .solution}
+>
+{: .question}
+
+## Sub-step with **dada2_plotQualityProfile**
+
+> ### {% icon hands_on %} Hands-on: Task description
+>
+> 1. **dada2_plotQualityProfile** {% icon tool %} with the following parameters:
+>
+>    ***TODO***: *Check parameter descriptions*
+>
+>    ***TODO***: *Consider adding a comment or tip box*
+>
+>    > ### {% icon comment %} Comment
+>    >
+>    > A comment about the tool or something else. This box can also be in the main text
+>    {: .comment}
+>
+{: .hands_on}
+
+***TODO***: *Consider adding a question to test the learners understanding of the previous exercise*
+
+> ### {% icon question %} Questions
+>
+> 1. Question1?
+> 2. Question2?
+>
+> > ### {% icon solution %} Solution
+> >
+> > 1. Answer for question1
+> > 2. Answer for question2
+> >
+> {: .solution}
+>
+{: .question}
+
+## Sub-step with **dada2_filterAndTrim**
+
+> ### {% icon hands_on %} Hands-on: Task description
+>
+> 1. **dada2_filterAndTrim** {% icon tool %} with the following parameters:
+>
+>    ***TODO***: *Check parameter descriptions*
+>
+>    ***TODO***: *Consider adding a comment or tip box*
+>
+>    > ### {% icon comment %} Comment
+>    >
+>    > A comment about the tool or something else. This box can also be in the main text
+>    {: .comment}
+>
+{: .hands_on}
+
+***TODO***: *Consider adding a question to test the learners understanding of the previous exercise*
+
+> ### {% icon question %} Questions
+>
+> 1. Question1?
+> 2. Question2?
+>
+> > ### {% icon solution %} Solution
+> >
+> > 1. Answer for question1
+> > 2. Answer for question2
+> >
+> {: .solution}
+>
+{: .question}
+
+## Sub-step with **dada2_plotComplexity**
+
+> ### {% icon hands_on %} Hands-on: Task description
+>
+> 1. **dada2_plotComplexity** {% icon tool %} with the following parameters:
+>
+>    ***TODO***: *Check parameter descriptions*
+>
+>    ***TODO***: *Consider adding a comment or tip box*
+>
+>    > ### {% icon comment %} Comment
+>    >
+>    > A comment about the tool or something else. This box can also be in the main text
+>    {: .comment}
+>
+{: .hands_on}
+
+***TODO***: *Consider adding a question to test the learners understanding of the previous exercise*
+
+> ### {% icon question %} Questions
+>
+> 1. Question1?
+> 2. Question2?
+>
+> > ### {% icon solution %} Solution
+> >
+> > 1. Answer for question1
+> > 2. Answer for question2
+> >
+> {: .solution}
+>
+{: .question}
+
+## Sub-step with **dada2_plotQualityProfile**
+
+> ### {% icon hands_on %} Hands-on: Task description
+>
+> 1. **dada2_plotQualityProfile** {% icon tool %} with the following parameters:
+>
+>    ***TODO***: *Check parameter descriptions*
+>
+>    ***TODO***: *Consider adding a comment or tip box*
+>
+>    > ### {% icon comment %} Comment
+>    >
+>    > A comment about the tool or something else. This box can also be in the main text
+>    {: .comment}
+>
+{: .hands_on}
+
+***TODO***: *Consider adding a question to test the learners understanding of the previous exercise*
+
+> ### {% icon question %} Questions
+>
+> 1. Question1?
+> 2. Question2?
+>
+> > ### {% icon solution %} Solution
+> >
+> > 1. Answer for question1
+> > 2. Answer for question2
+> >
+> {: .solution}
+>
+{: .question}
+
+## Sub-step with **Unzip Collection**
+
+> ### {% icon hands_on %} Hands-on: Task description
+>
+> 1. **Unzip Collection** {% icon tool %} with the following parameters:
+>    - {% icon param-file %} *"Input Paired Dataset"*: `paired_output` (output of **dada2_filterAndTrim** {% icon tool %})
+>
+>    ***TODO***: *Check parameter descriptions*
+>
+>    ***TODO***: *Consider adding a comment or tip box*
+>
+>    > ### {% icon comment %} Comment
+>    >
+>    > A comment about the tool or something else. This box can also be in the main text
+>    {: .comment}
+>
+{: .hands_on}
+
+***TODO***: *Consider adding a question to test the learners understanding of the previous exercise*
+
+> ### {% icon question %} Questions
+>
+> 1. Question1?
+> 2. Question2?
+>
+> > ### {% icon solution %} Solution
+> >
+> > 1. Answer for question1
+> > 2. Answer for question2
+> >
+> {: .solution}
+>
+{: .question}
+
+## Sub-step with **dada2_learnErrors**
+
+> ### {% icon hands_on %} Hands-on: Task description
+>
+> 1. **dada2_learnErrors** {% icon tool %} with the following parameters:
+>
+>    ***TODO***: *Check parameter descriptions*
+>
+>    ***TODO***: *Consider adding a comment or tip box*
+>
+>    > ### {% icon comment %} Comment
+>    >
+>    > A comment about the tool or something else. This box can also be in the main text
+>    {: .comment}
+>
+{: .hands_on}
+
+***TODO***: *Consider adding a question to test the learners understanding of the previous exercise*
+
+> ### {% icon question %} Questions
+>
+> 1. Question1?
+> 2. Question2?
+>
+> > ### {% icon solution %} Solution
+> >
+> > 1. Answer for question1
+> > 2. Answer for question2
+> >
+> {: .solution}
+>
+{: .question}
+
+## Sub-step with **dada2_learnErrors**
+
+> ### {% icon hands_on %} Hands-on: Task description
+>
+> 1. **dada2_learnErrors** {% icon tool %} with the following parameters:
+>
+>    ***TODO***: *Check parameter descriptions*
+>
+>    ***TODO***: *Consider adding a comment or tip box*
+>
+>    > ### {% icon comment %} Comment
+>    >
+>    > A comment about the tool or something else. This box can also be in the main text
+>    {: .comment}
+>
+{: .hands_on}
+
+***TODO***: *Consider adding a question to test the learners understanding of the previous exercise*
+
+> ### {% icon question %} Questions
+>
+> 1. Question1?
+> 2. Question2?
+>
+> > ### {% icon solution %} Solution
+> >
+> > 1. Answer for question1
+> > 2. Answer for question2
+> >
+> {: .solution}
+>
+{: .question}
+
+## Sub-step with **dada2_dada**
+
+> ### {% icon hands_on %} Hands-on: Task description
+>
+> 1. **dada2_dada** {% icon tool %} with the following parameters:
+>
+>    ***TODO***: *Check parameter descriptions*
+>
+>    ***TODO***: *Consider adding a comment or tip box*
+>
+>    > ### {% icon comment %} Comment
+>    >
+>    > A comment about the tool or something else. This box can also be in the main text
+>    {: .comment}
+>
+{: .hands_on}
+
+***TODO***: *Consider adding a question to test the learners understanding of the previous exercise*
+
+> ### {% icon question %} Questions
+>
+> 1. Question1?
+> 2. Question2?
+>
+> > ### {% icon solution %} Solution
+> >
+> > 1. Answer for question1
+> > 2. Answer for question2
+> >
+> {: .solution}
+>
+{: .question}
+
+## Sub-step with **dada2_dada**
+
+> ### {% icon hands_on %} Hands-on: Task description
+>
+> 1. **dada2_dada** {% icon tool %} with the following parameters:
+>
+>    ***TODO***: *Check parameter descriptions*
+>
+>    ***TODO***: *Consider adding a comment or tip box*
+>
+>    > ### {% icon comment %} Comment
+>    >
+>    > A comment about the tool or something else. This box can also be in the main text
+>    {: .comment}
+>
+{: .hands_on}
+
+***TODO***: *Consider adding a question to test the learners understanding of the previous exercise*
+
+> ### {% icon question %} Questions
+>
+> 1. Question1?
+> 2. Question2?
+>
+> > ### {% icon solution %} Solution
+> >
+> > 1. Answer for question1
+> > 2. Answer for question2
+> >
+> {: .solution}
+>
+{: .question}
+
+## Sub-step with **dada2_mergePairs**
+
+> ### {% icon hands_on %} Hands-on: Task description
+>
+> 1. **dada2_mergePairs** {% icon tool %} with the following parameters:
+>
+>    ***TODO***: *Check parameter descriptions*
+>
+>    ***TODO***: *Consider adding a comment or tip box*
+>
+>    > ### {% icon comment %} Comment
+>    >
+>    > A comment about the tool or something else. This box can also be in the main text
+>    {: .comment}
+>
+{: .hands_on}
+
+***TODO***: *Consider adding a question to test the learners understanding of the previous exercise*
+
+> ### {% icon question %} Questions
+>
+> 1. Question1?
+> 2. Question2?
+>
+> > ### {% icon solution %} Solution
+> >
+> > 1. Answer for question1
+> > 2. Answer for question2
+> >
+> {: .solution}
+>
+{: .question}
+
+## Sub-step with **dada2_makeSequenceTable**
+
+> ### {% icon hands_on %} Hands-on: Task description
+>
+> 1. **dada2_makeSequenceTable** {% icon tool %} with the following parameters:
+>
+>    ***TODO***: *Check parameter descriptions*
+>
+>    ***TODO***: *Consider adding a comment or tip box*
+>
+>    > ### {% icon comment %} Comment
+>    >
+>    > A comment about the tool or something else. This box can also be in the main text
+>    {: .comment}
+>
+{: .hands_on}
+
+***TODO***: *Consider adding a question to test the learners understanding of the previous exercise*
+
+> ### {% icon question %} Questions
+>
+> 1. Question1?
+> 2. Question2?
+>
+> > ### {% icon solution %} Solution
+> >
+> > 1. Answer for question1
+> > 2. Answer for question2
+> >
+> {: .solution}
+>
+{: .question}
+
+## Sub-step with **dada2_removeBimeraDenovo**
+
+> ### {% icon hands_on %} Hands-on: Task description
+>
+> 1. **dada2_removeBimeraDenovo** {% icon tool %} with the following parameters:
+>
+>    ***TODO***: *Check parameter descriptions*
+>
+>    ***TODO***: *Consider adding a comment or tip box*
+>
+>    > ### {% icon comment %} Comment
+>    >
+>    > A comment about the tool or something else. This box can also be in the main text
+>    {: .comment}
+>
+{: .hands_on}
+
+***TODO***: *Consider adding a question to test the learners understanding of the previous exercise*
+
+> ### {% icon question %} Questions
+>
+> 1. Question1?
+> 2. Question2?
+>
+> > ### {% icon solution %} Solution
+> >
+> > 1. Answer for question1
+> > 2. Answer for question2
+> >
+> {: .solution}
+>
+{: .question}
+
+## Sub-step with **dada2_seqCounts**
+
+> ### {% icon hands_on %} Hands-on: Task description
+>
+> 1. **dada2_seqCounts** {% icon tool %} with the following parameters:
+>
+>    ***TODO***: *Check parameter descriptions*
+>
+>    ***TODO***: *Consider adding a comment or tip box*
+>
+>    > ### {% icon comment %} Comment
+>    >
+>    > A comment about the tool or something else. This box can also be in the main text
+>    {: .comment}
+>
+{: .hands_on}
+
+***TODO***: *Consider adding a question to test the learners understanding of the previous exercise*
+
+> ### {% icon question %} Questions
+>
+> 1. Question1?
+> 2. Question2?
+>
+> > ### {% icon solution %} Solution
+> >
+> > 1. Answer for question1
+> > 2. Answer for question2
+> >
+> {: .solution}
+>
+{: .question}
+
+## Sub-step with **dada2_assignTaxonomyAddspecies**
+
+> ### {% icon hands_on %} Hands-on: Task description
+>
+> 1. **dada2_assignTaxonomyAddspecies** {% icon tool %} with the following parameters:
+>
+>    ***TODO***: *Check parameter descriptions*
+>
+>    ***TODO***: *Consider adding a comment or tip box*
+>
+>    > ### {% icon comment %} Comment
+>    >
+>    > A comment about the tool or something else. This box can also be in the main text
+>    {: .comment}
+>
+{: .hands_on}
+
+***TODO***: *Consider adding a question to test the learners understanding of the previous exercise*
+
+> ### {% icon question %} Questions
+>
+> 1. Question1?
+> 2. Question2?
+>
+> > ### {% icon solution %} Solution
+> >
+> > 1. Answer for question1
+> > 2. Answer for question2
+> >
+> {: .solution}
+>
+{: .question}
+
+
+## Re-arrange
+
+To create the template, each step of the workflow had its own subsection.
+
+***TODO***: *Re-arrange the generated subsections into sections or other subsections.
+Consider merging some hands-on boxes to have a meaningful flow of the analyses*
+
+# Conclusion
+{:.no_toc}
+
+Sum up the tutorial and the key takeaways here. We encourage adding an overview image of the
+pipeline used.
\ No newline at end of file
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/topics/metagenomics/tutorials/dada2/workflows/main_workflow.ga	Mon Feb 01 20:22:27 2021 +0000
@@ -0,0 +1,798 @@
+{
+    "a_galaxy_workflow": "true", 
+    "annotation": "", 
+    "format-version": "0.1", 
+    "name": "dada2 for paired data (imported from uploaded file)", 
+    "steps": {
+        "0": {
+            "annotation": "", 
+            "content_id": null, 
+            "errors": null, 
+            "id": 0, 
+            "input_connections": {}, 
+            "inputs": [
+                {
+                    "description": "", 
+                    "name": "Input Dataset Collection"
+                }
+            ], 
+            "label": null, 
+            "name": "Input dataset collection", 
+            "outputs": [], 
+            "position": {
+                "left": 200, 
+                "top": 613
+            }, 
+            "tool_id": null, 
+            "tool_state": "{\"collection_type\": \"list:paired\", \"name\": \"Input Dataset Collection\"}", 
+            "tool_version": null, 
+            "type": "data_collection_input", 
+            "uuid": "b1f16224-49d2-4b7a-a37d-3d93c54f679f", 
+            "workflow_outputs": []
+        }, 
+        "1": {
+            "annotation": "", 
+            "content_id": "dada2_plotComplexity", 
+            "errors": null, 
+            "id": 1, 
+            "input_connections": {
+                "batch_cond|paired_cond|reads": {
+                    "id": 0, 
+                    "output_name": "output"
+                }
+            }, 
+            "inputs": [], 
+            "label": null, 
+            "name": "dada2: plotComplexity", 
+            "outputs": [
+                {
+                    "name": "output_fwd", 
+                    "type": "pdf"
+                }, 
+                {
+                    "name": "output_rev", 
+                    "type": "pdf"
+                }
+            ], 
+            "position": {
+                "left": 499, 
+                "top": 178.5
+            }, 
+            "post_job_actions": {
+                "HideDatasetActionoutput_fwd": {
+                    "action_arguments": {}, 
+                    "action_type": "HideDatasetAction", 
+                    "output_name": "output_fwd"
+                }, 
+                "HideDatasetActionoutput_rev": {
+                    "action_arguments": {}, 
+                    "action_type": "HideDatasetAction", 
+                    "output_name": "output_rev"
+                }
+            }, 
+            "tool_id": "dada2_plotComplexity", 
+            "tool_state": "{\"__page__\": null, \"__rerun_remap_job_id__\": null, \"kmerSize\": \"\\\"2\\\"\", \"n\": \"\\\"100000\\\"\", \"window\": \"\\\"\\\"\", \"batch_cond\": \"{\\\"__current_case__\\\": 0, \\\"aggregate\\\": \\\"true\\\", \\\"batch_select\\\": \\\"joint\\\", \\\"paired_cond\\\": {\\\"__current_case__\\\": 0, \\\"paired_select\\\": \\\"paired\\\", \\\"reads\\\": {\\\"__class__\\\": \\\"RuntimeValue\\\"}}}\", \"by\": \"\\\"5\\\"\", \"bins\": \"\\\"100\\\"\"}", 
+            "tool_version": "1.12+galaxy0", 
+            "type": "tool", 
+            "uuid": "4f226c7b-9ba8-4b48-8d48-899cf345b660", 
+            "workflow_outputs": [
+                {
+                    "label": null, 
+                    "output_name": "output_fwd", 
+                    "uuid": "52b4da66-504c-4fbe-8487-d6c0613ab6ae"
+                }, 
+                {
+                    "label": null, 
+                    "output_name": "output_rev", 
+                    "uuid": "3785a6f5-da8a-4232-b2ab-4f9f4c0732f6"
+                }
+            ]
+        }, 
+        "2": {
+            "annotation": "", 
+            "content_id": "dada2_plotQualityProfile", 
+            "errors": null, 
+            "id": 2, 
+            "input_connections": {
+                "batch_cond|paired_cond|reads": {
+                    "id": 0, 
+                    "output_name": "output"
+                }
+            }, 
+            "inputs": [], 
+            "label": null, 
+            "name": "dada2: plotQualityProfile", 
+            "outputs": [
+                {
+                    "name": "output_fwd", 
+                    "type": "pdf"
+                }, 
+                {
+                    "name": "output_rev", 
+                    "type": "pdf"
+                }
+            ], 
+            "position": {
+                "left": 496, 
+                "top": 364
+            }, 
+            "post_job_actions": {
+                "HideDatasetActionoutput_fwd": {
+                    "action_arguments": {}, 
+                    "action_type": "HideDatasetAction", 
+                    "output_name": "output_fwd"
+                }, 
+                "HideDatasetActionoutput_rev": {
+                    "action_arguments": {}, 
+                    "action_type": "HideDatasetAction", 
+                    "output_name": "output_rev"
+                }
+            }, 
+            "tool_id": "dada2_plotQualityProfile", 
+            "tool_state": "{\"__page__\": null, \"__rerun_remap_job_id__\": null, \"paired_cond\": \"{\\\"__current_case__\\\": 0, \\\"paired_select\\\": \\\"paired\\\", \\\"reads\\\": {\\\"__class__\\\": \\\"ConnectedValue\\\"}}\", \"n\": \"\\\"500000\\\"\", \"batch_cond\": \"{\\\"__current_case__\\\": 0, \\\"aggregate\\\": \\\"true\\\", \\\"batch_select\\\": \\\"joint\\\", \\\"paired_cond\\\": {\\\"__current_case__\\\": 0, \\\"paired_select\\\": \\\"paired\\\", \\\"reads\\\": {\\\"__class__\\\": \\\"RuntimeValue\\\"}}}\", \"aggregate\": \"\\\"true\\\"\", \"chromInfo\": \"\\\"/home/berntm/projects/galaxy/tool-data/shared/ucsc/chrom/?.len\\\"\"}", 
+            "tool_version": "1.12+galaxy0", 
+            "type": "tool", 
+            "uuid": "f6e0a2ea-a639-48d9-ae3c-c1ec2bab93e5", 
+            "workflow_outputs": []
+        }, 
+        "3": {
+            "annotation": "", 
+            "content_id": "dada2_filterAndTrim", 
+            "errors": null, 
+            "id": 3, 
+            "input_connections": {
+                "paired_cond|reads": {
+                    "id": 0, 
+                    "output_name": "output"
+                }
+            }, 
+            "inputs": [], 
+            "label": null, 
+            "name": "dada2: filterAndTrim", 
+            "outputs": [
+                {
+                    "name": "paired_output", 
+                    "type": "input"
+                }, 
+                {
+                    "name": "outtab", 
+                    "type": "tabular"
+                }
+            ], 
+            "position": {
+                "left": 485, 
+                "top": 612
+            }, 
+            "post_job_actions": {
+                "HideDatasetActionouttab": {
+                    "action_arguments": {}, 
+                    "action_type": "HideDatasetAction", 
+                    "output_name": "outtab"
+                }, 
+                "HideDatasetActionpaired_output": {
+                    "action_arguments": {}, 
+                    "action_type": "HideDatasetAction", 
+                    "output_name": "paired_output"
+                }
+            }, 
+            "tool_id": "dada2_filterAndTrim", 
+            "tool_state": "{\"trim\": \"{\\\"trimLeft\\\": \\\"0\\\", \\\"trimRight\\\": \\\"0\\\", \\\"truncLen\\\": \\\"0\\\", \\\"truncQ\\\": \\\"2\\\"}\", \"__page__\": null, \"rmPhiX\": \"\\\"true\\\"\", \"rmlowcomplex\": \"\\\"0\\\"\", \"__rerun_remap_job_id__\": null, \"paired_cond\": \"{\\\"__current_case__\\\": 0, \\\"paired_select\\\": \\\"paired\\\", \\\"reads\\\": {\\\"__class__\\\": \\\"ConnectedValue\\\"}}\", \"filter\": \"{\\\"maxEE\\\": \\\"\\\", \\\"maxLen\\\": \\\"\\\", \\\"maxN\\\": \\\"0\\\", \\\"minLen\\\": \\\"20\\\", \\\"minQ\\\": \\\"0\\\"}\", \"seprev_cond\": \"{\\\"__current_case__\\\": 0, \\\"seprev_select\\\": \\\"no\\\"}\", \"output_statistics\": \"\\\"true\\\"\", \"orientFwd\": \"\\\"\\\"\", \"chromInfo\": \"\\\"/home/berntm/projects/galaxy/tool-data/shared/ucsc/chrom/?.len\\\"\"}", 
+            "tool_version": "1.12+galaxy0", 
+            "type": "tool", 
+            "uuid": "353103fd-afc3-4fe0-a4a3-8dca75b59107", 
+            "workflow_outputs": []
+        }, 
+        "4": {
+            "annotation": "", 
+            "content_id": "dada2_plotComplexity", 
+            "errors": null, 
+            "id": 4, 
+            "input_connections": {
+                "batch_cond|paired_cond|reads": {
+                    "id": 3, 
+                    "output_name": "paired_output"
+                }
+            }, 
+            "inputs": [], 
+            "label": null, 
+            "name": "dada2: plotComplexity", 
+            "outputs": [
+                {
+                    "name": "output_fwd", 
+                    "type": "pdf"
+                }, 
+                {
+                    "name": "output_rev", 
+                    "type": "pdf"
+                }
+            ], 
+            "position": {
+                "left": 840, 
+                "top": 179.5
+            }, 
+            "post_job_actions": {
+                "HideDatasetActionoutput_fwd": {
+                    "action_arguments": {}, 
+                    "action_type": "HideDatasetAction", 
+                    "output_name": "output_fwd"
+                }, 
+                "HideDatasetActionoutput_rev": {
+                    "action_arguments": {}, 
+                    "action_type": "HideDatasetAction", 
+                    "output_name": "output_rev"
+                }
+            }, 
+            "tool_id": "dada2_plotComplexity", 
+            "tool_state": "{\"__page__\": null, \"__rerun_remap_job_id__\": null, \"kmerSize\": \"\\\"2\\\"\", \"n\": \"\\\"100000\\\"\", \"window\": \"\\\"\\\"\", \"batch_cond\": \"{\\\"__current_case__\\\": 0, \\\"aggregate\\\": \\\"true\\\", \\\"batch_select\\\": \\\"joint\\\", \\\"paired_cond\\\": {\\\"__current_case__\\\": 0, \\\"paired_select\\\": \\\"paired\\\", \\\"reads\\\": {\\\"__class__\\\": \\\"RuntimeValue\\\"}}}\", \"by\": \"\\\"5\\\"\", \"bins\": \"\\\"100\\\"\"}", 
+            "tool_version": "1.12+galaxy0", 
+            "type": "tool", 
+            "uuid": "9c6c20be-b9d8-4645-a2d6-3ecc5bc33f4a", 
+            "workflow_outputs": []
+        }, 
+        "5": {
+            "annotation": "", 
+            "content_id": "dada2_plotQualityProfile", 
+            "errors": null, 
+            "id": 5, 
+            "input_connections": {
+                "batch_cond|paired_cond|reads": {
+                    "id": 3, 
+                    "output_name": "paired_output"
+                }
+            }, 
+            "inputs": [], 
+            "label": null, 
+            "name": "dada2: plotQualityProfile", 
+            "outputs": [
+                {
+                    "name": "output_fwd", 
+                    "type": "pdf"
+                }, 
+                {
+                    "name": "output_rev", 
+                    "type": "pdf"
+                }
+            ], 
+            "position": {
+                "left": 842.5, 
+                "top": 364.5
+            }, 
+            "post_job_actions": {
+                "HideDatasetActionoutput_fwd": {
+                    "action_arguments": {}, 
+                    "action_type": "HideDatasetAction", 
+                    "output_name": "output_fwd"
+                }, 
+                "HideDatasetActionoutput_rev": {
+                    "action_arguments": {}, 
+                    "action_type": "HideDatasetAction", 
+                    "output_name": "output_rev"
+                }
+            }, 
+            "tool_id": "dada2_plotQualityProfile", 
+            "tool_state": "{\"__page__\": null, \"__rerun_remap_job_id__\": null, \"paired_cond\": \"{\\\"__current_case__\\\": 0, \\\"paired_select\\\": \\\"paired\\\", \\\"reads\\\": {\\\"__class__\\\": \\\"ConnectedValue\\\"}}\", \"n\": \"\\\"500000\\\"\", \"batch_cond\": \"{\\\"__current_case__\\\": 0, \\\"aggregate\\\": \\\"true\\\", \\\"batch_select\\\": \\\"joint\\\", \\\"paired_cond\\\": {\\\"__current_case__\\\": 0, \\\"paired_select\\\": \\\"paired\\\", \\\"reads\\\": {\\\"__class__\\\": \\\"RuntimeValue\\\"}}}\", \"aggregate\": \"\\\"true\\\"\", \"chromInfo\": \"\\\"/home/berntm/projects/galaxy/tool-data/shared/ucsc/chrom/?.len\\\"\"}", 
+            "tool_version": "1.12+galaxy0", 
+            "type": "tool", 
+            "uuid": "b5234046-b9a9-4801-8994-496173c3ab2b", 
+            "workflow_outputs": []
+        }, 
+        "6": {
+            "annotation": "", 
+            "content_id": "__UNZIP_COLLECTION__", 
+            "errors": null, 
+            "id": 6, 
+            "input_connections": {
+                "input": {
+                    "id": 3, 
+                    "output_name": "paired_output"
+                }
+            }, 
+            "inputs": [], 
+            "label": null, 
+            "name": "Unzip Collection", 
+            "outputs": [
+                {
+                    "name": "forward", 
+                    "type": "input"
+                }, 
+                {
+                    "name": "reverse", 
+                    "type": "input"
+                }
+            ], 
+            "position": {
+                "left": 841, 
+                "top": 636
+            }, 
+            "post_job_actions": {
+                "HideDatasetActionforward": {
+                    "action_arguments": {}, 
+                    "action_type": "HideDatasetAction", 
+                    "output_name": "forward"
+                }, 
+                "HideDatasetActionreverse": {
+                    "action_arguments": {}, 
+                    "action_type": "HideDatasetAction", 
+                    "output_name": "reverse"
+                }
+            }, 
+            "tool_id": "__UNZIP_COLLECTION__", 
+            "tool_state": "{\"input\": \"{\\\"__class__\\\": \\\"ConnectedValue\\\"}\", \"__rerun_remap_job_id__\": null, \"__page__\": null}", 
+            "tool_version": "1.0.0", 
+            "type": "tool", 
+            "uuid": "41ab27fe-f07d-4653-b4b5-e77408957a9a", 
+            "workflow_outputs": []
+        }, 
+        "7": {
+            "annotation": "", 
+            "content_id": "dada2_learnErrors", 
+            "errors": null, 
+            "id": 7, 
+            "input_connections": {
+                "fls": {
+                    "id": 6, 
+                    "output_name": "forward"
+                }
+            }, 
+            "inputs": [
+                {
+                    "description": "runtime parameter for tool dada2: learnErrors", 
+                    "name": "fls"
+                }
+            ], 
+            "label": null, 
+            "name": "dada2: learnErrors", 
+            "outputs": [
+                {
+                    "name": "errors", 
+                    "type": "dada2_errorrates"
+                }, 
+                {
+                    "name": "plot", 
+                    "type": "pdf"
+                }
+            ], 
+            "position": {
+                "left": 1152, 
+                "top": 445
+            }, 
+            "post_job_actions": {
+                "HideDatasetActionerrors": {
+                    "action_arguments": {}, 
+                    "action_type": "HideDatasetAction", 
+                    "output_name": "errors"
+                }, 
+                "HideDatasetActionplot": {
+                    "action_arguments": {}, 
+                    "action_type": "HideDatasetAction", 
+                    "output_name": "plot"
+                }
+            }, 
+            "tool_id": "dada2_learnErrors", 
+            "tool_state": "{\"__page__\": null, \"plotopt\": \"{\\\"err_in\\\": \\\"false\\\", \\\"err_out\\\": \\\"true\\\", \\\"nominalQ\\\": \\\"true\\\", \\\"obs\\\": \\\"true\\\"}\", \"fls\": \"{\\\"__class__\\\": \\\"RuntimeValue\\\"}\", \"reads\": \"{\\\"__class__\\\": \\\"ConnectedValue\\\"}\", \"nbases\": \"\\\"8\\\"\", \"chromInfo\": \"\\\"/home/berntm/projects/galaxy/tool-data/shared/ucsc/chrom/?.len\\\"\", \"__rerun_remap_job_id__\": null, \"advanced\": \"{\\\"errfoo\\\": \\\"loessErrfun\\\", \\\"maxconsist\\\": \\\"10\\\", \\\"omegac\\\": \\\"0.0\\\", \\\"randomize\\\": \\\"false\\\"}\"}", 
+            "tool_version": "1.12+galaxy0", 
+            "type": "tool", 
+            "uuid": "e8c86ffd-118c-44f6-a58d-5c1704bb6ed6", 
+            "workflow_outputs": []
+        }, 
+        "8": {
+            "annotation": "", 
+            "content_id": "dada2_learnErrors", 
+            "errors": null, 
+            "id": 8, 
+            "input_connections": {
+                "fls": {
+                    "id": 6, 
+                    "output_name": "reverse"
+                }
+            }, 
+            "inputs": [
+                {
+                    "description": "runtime parameter for tool dada2: learnErrors", 
+                    "name": "fls"
+                }
+            ], 
+            "label": null, 
+            "name": "dada2: learnErrors", 
+            "outputs": [
+                {
+                    "name": "errors", 
+                    "type": "dada2_errorrates"
+                }, 
+                {
+                    "name": "plot", 
+                    "type": "pdf"
+                }
+            ], 
+            "position": {
+                "left": 1109, 
+                "top": 871
+            }, 
+            "post_job_actions": {
+                "HideDatasetActionerrors": {
+                    "action_arguments": {}, 
+                    "action_type": "HideDatasetAction", 
+                    "output_name": "errors"
+                }, 
+                "HideDatasetActionplot": {
+                    "action_arguments": {}, 
+                    "action_type": "HideDatasetAction", 
+                    "output_name": "plot"
+                }
+            }, 
+            "tool_id": "dada2_learnErrors", 
+            "tool_state": "{\"__page__\": null, \"plotopt\": \"{\\\"err_in\\\": \\\"false\\\", \\\"err_out\\\": \\\"true\\\", \\\"nominalQ\\\": \\\"true\\\", \\\"obs\\\": \\\"true\\\"}\", \"fls\": \"{\\\"__class__\\\": \\\"RuntimeValue\\\"}\", \"reads\": \"{\\\"__class__\\\": \\\"ConnectedValue\\\"}\", \"nbases\": \"\\\"8\\\"\", \"chromInfo\": \"\\\"/home/berntm/projects/galaxy/tool-data/shared/ucsc/chrom/?.len\\\"\", \"__rerun_remap_job_id__\": null, \"advanced\": \"{\\\"errfoo\\\": \\\"loessErrfun\\\", \\\"maxconsist\\\": \\\"10\\\", \\\"omegac\\\": \\\"0.0\\\", \\\"randomize\\\": \\\"false\\\"}\"}", 
+            "tool_version": "1.12+galaxy0", 
+            "type": "tool", 
+            "uuid": "2cbc5056-7cc7-4e3e-a283-959a261ca4c5", 
+            "workflow_outputs": []
+        }, 
+        "9": {
+            "annotation": "", 
+            "content_id": "dada2_dada", 
+            "errors": null, 
+            "id": 9, 
+            "input_connections": {
+                "batch_cond|derep": {
+                    "id": 6, 
+                    "output_name": "forward"
+                }, 
+                "err": {
+                    "id": 7, 
+                    "output_name": "errors"
+                }
+            }, 
+            "inputs": [
+                {
+                    "description": "runtime parameter for tool dada2: dada", 
+                    "name": "batch_cond"
+                }, 
+                {
+                    "description": "runtime parameter for tool dada2: dada", 
+                    "name": "err"
+                }
+            ], 
+            "label": null, 
+            "name": "dada2: dada", 
+            "outputs": [
+                {
+                    "name": "data_collection", 
+                    "type": "input"
+                }
+            ], 
+            "position": {
+                "left": 1424.5, 
+                "top": 585.5
+            }, 
+            "post_job_actions": {}, 
+            "tool_id": "dada2_dada", 
+            "tool_state": "{\"__page__\": null, \"__rerun_remap_job_id__\": null, \"batch_cond\": \"{\\\"__current_case__\\\": 1, \\\"batch_select\\\": \\\"no\\\", \\\"derep\\\": {\\\"__class__\\\": \\\"RuntimeValue\\\"}, \\\"pool\\\": \\\"FALSE\\\"}\", \"err\": \"{\\\"__class__\\\": \\\"RuntimeValue\\\"}\"}", 
+            "tool_version": "1.12+galaxy0", 
+            "type": "tool", 
+            "uuid": "b51bcd73-c8f0-4c39-8a23-9a5baa0a6b4f", 
+            "workflow_outputs": [
+                {
+                    "label": null, 
+                    "output_name": "data_collection", 
+                    "uuid": "8b97daeb-3389-4140-929d-593c5d8bba3a"
+                }
+            ]
+        }, 
+        "10": {
+            "annotation": "", 
+            "content_id": "dada2_dada", 
+            "errors": null, 
+            "id": 10, 
+            "input_connections": {
+                "batch_cond|derep": {
+                    "id": 6, 
+                    "output_name": "reverse"
+                }, 
+                "err": {
+                    "id": 8, 
+                    "output_name": "errors"
+                }
+            }, 
+            "inputs": [
+                {
+                    "description": "runtime parameter for tool dada2: dada", 
+                    "name": "batch_cond"
+                }, 
+                {
+                    "description": "runtime parameter for tool dada2: dada", 
+                    "name": "err"
+                }
+            ], 
+            "label": null, 
+            "name": "dada2: dada", 
+            "outputs": [
+                {
+                    "name": "data_collection", 
+                    "type": "input"
+                }
+            ], 
+            "position": {
+                "left": 1413.5, 
+                "top": 803.5
+            }, 
+            "post_job_actions": {}, 
+            "tool_id": "dada2_dada", 
+            "tool_state": "{\"__page__\": null, \"__rerun_remap_job_id__\": null, \"batch_cond\": \"{\\\"__current_case__\\\": 1, \\\"batch_select\\\": \\\"no\\\", \\\"derep\\\": {\\\"__class__\\\": \\\"RuntimeValue\\\"}, \\\"pool\\\": \\\"FALSE\\\"}\", \"err\": \"{\\\"__class__\\\": \\\"RuntimeValue\\\"}\"}", 
+            "tool_version": "1.12+galaxy0", 
+            "type": "tool", 
+            "uuid": "188c4ee7-3fb7-4dc7-8efd-30fb38c0d679", 
+            "workflow_outputs": [
+                {
+                    "label": null, 
+                    "output_name": "data_collection", 
+                    "uuid": "7dfc0db1-2376-440c-ac0b-3f9932f063db"
+                }
+            ]
+        }, 
+        "11": {
+            "annotation": "", 
+            "content_id": "dada2_mergePairs", 
+            "errors": null, 
+            "id": 11, 
+            "input_connections": {
+                "dadaF": {
+                    "id": 9, 
+                    "output_name": "data_collection"
+                }, 
+                "dadaR": {
+                    "id": 10, 
+                    "output_name": "data_collection"
+                }, 
+                "derepF": {
+                    "id": 6, 
+                    "output_name": "forward"
+                }, 
+                "derepR": {
+                    "id": 6, 
+                    "output_name": "reverse"
+                }
+            }, 
+            "inputs": [
+                {
+                    "description": "runtime parameter for tool dada2: mergePairs", 
+                    "name": "dadaR"
+                }, 
+                {
+                    "description": "runtime parameter for tool dada2: mergePairs", 
+                    "name": "derepR"
+                }, 
+                {
+                    "description": "runtime parameter for tool dada2: mergePairs", 
+                    "name": "dadaF"
+                }, 
+                {
+                    "description": "runtime parameter for tool dada2: mergePairs", 
+                    "name": "derepF"
+                }
+            ], 
+            "label": null, 
+            "name": "dada2: mergePairs", 
+            "outputs": [
+                {
+                    "name": "merged", 
+                    "type": "dada2_mergepairs"
+                }
+            ], 
+            "position": {
+                "left": 1691, 
+                "top": 652.5
+            }, 
+            "post_job_actions": {}, 
+            "tool_id": "dada2_mergePairs", 
+            "tool_state": "{\"__page__\": null, \"dadaF\": \"{\\\"__class__\\\": \\\"RuntimeValue\\\"}\", \"derepR\": \"{\\\"__class__\\\": \\\"RuntimeValue\\\"}\", \"maxMismatch\": \"\\\"0\\\"\", \"trimOverhang\": \"\\\"false\\\"\", \"minOverlap\": \"\\\"12\\\"\", \"justConcatenate\": \"\\\"false\\\"\", \"dadaR\": \"{\\\"__class__\\\": \\\"RuntimeValue\\\"}\", \"__rerun_remap_job_id__\": null, \"derepF\": \"{\\\"__class__\\\": \\\"RuntimeValue\\\"}\"}", 
+            "tool_version": "1.12+galaxy0", 
+            "type": "tool", 
+            "uuid": "8a74535a-c7a2-4c24-a907-8cb791b779bf", 
+            "workflow_outputs": [
+                {
+                    "label": null, 
+                    "output_name": "merged", 
+                    "uuid": "887453f1-44ea-4882-8b94-91a3b02eaa0d"
+                }
+            ]
+        }, 
+        "12": {
+            "annotation": "", 
+            "content_id": "dada2_makeSequenceTable", 
+            "errors": null, 
+            "id": 12, 
+            "input_connections": {
+                "samples": {
+                    "id": 11, 
+                    "output_name": "merged"
+                }
+            }, 
+            "inputs": [], 
+            "label": null, 
+            "name": "dada2: makeSequenceTable", 
+            "outputs": [
+                {
+                    "name": "stable", 
+                    "type": "dada2_sequencetable"
+                }, 
+                {
+                    "name": "plot_output", 
+                    "type": "pdf"
+                }
+            ], 
+            "position": {
+                "left": 1999, 
+                "top": 694
+            }, 
+            "post_job_actions": {
+                "HideDatasetActionplot_output": {
+                    "action_arguments": {}, 
+                    "action_type": "HideDatasetAction", 
+                    "output_name": "plot_output"
+                }, 
+                "HideDatasetActionstable": {
+                    "action_arguments": {}, 
+                    "action_type": "HideDatasetAction", 
+                    "output_name": "stable"
+                }
+            }, 
+            "tool_id": "dada2_makeSequenceTable", 
+            "tool_state": "{\"orderBy\": \"\\\"abundance\\\"\", \"plot\": \"\\\"true\\\"\", \"__page__\": null, \"filter_cond\": \"{\\\"__current_case__\\\": 0, \\\"filter_select\\\": \\\"no\\\"}\", \"samples\": \"{\\\"__class__\\\": \\\"ConnectedValue\\\"}\", \"chromInfo\": \"\\\"/home/berntm/projects/galaxy/tool-data/shared/ucsc/chrom/?.len\\\"\", \"__rerun_remap_job_id__\": null}", 
+            "tool_version": "1.12+galaxy0", 
+            "type": "tool", 
+            "uuid": "3cd64745-bb03-4bfa-b425-a16db0888967", 
+            "workflow_outputs": []
+        }, 
+        "13": {
+            "annotation": "", 
+            "content_id": "dada2_removeBimeraDenovo", 
+            "errors": null, 
+            "id": 13, 
+            "input_connections": {
+                "unqs": {
+                    "id": 12, 
+                    "output_name": "stable"
+                }
+            }, 
+            "inputs": [], 
+            "label": null, 
+            "name": "dada2: removeBimeraDenovo", 
+            "outputs": [
+                {
+                    "name": "stable_uniques", 
+                    "type": "dada2_uniques"
+                }, 
+                {
+                    "name": "stable_mergepairs", 
+                    "type": "dada2_mergepairs"
+                }, 
+                {
+                    "name": "stable_sequencetable", 
+                    "type": "dada2_sequencetable"
+                }
+            ], 
+            "position": {
+                "left": 2299, 
+                "top": 640
+            }, 
+            "post_job_actions": {
+                "HideDatasetActionstable_mergepairs": {
+                    "action_arguments": {}, 
+                    "action_type": "HideDatasetAction", 
+                    "output_name": "stable_mergepairs"
+                }, 
+                "HideDatasetActionstable_sequencetable": {
+                    "action_arguments": {}, 
+                    "action_type": "HideDatasetAction", 
+                    "output_name": "stable_sequencetable"
+                }, 
+                "HideDatasetActionstable_uniques": {
+                    "action_arguments": {}, 
+                    "action_type": "HideDatasetAction", 
+                    "output_name": "stable_uniques"
+                }
+            }, 
+            "tool_id": "dada2_removeBimeraDenovo", 
+            "tool_state": "{\"unqs\": \"{\\\"__class__\\\": \\\"ConnectedValue\\\"}\", \"__rerun_remap_job_id__\": null, \"chromInfo\": \"\\\"/home/berntm/projects/galaxy/tool-data/shared/ucsc/chrom/?.len\\\"\", \"method\": \"\\\"consensus\\\"\", \"__page__\": null}", 
+            "tool_version": "1.12+galaxy0", 
+            "type": "tool", 
+            "uuid": "4c001b98-d20d-4255-96ee-2b9829aa815b", 
+            "workflow_outputs": []
+        }, 
+        "14": {
+            "annotation": "", 
+            "content_id": "dada2_seqCounts", 
+            "errors": null, 
+            "id": 14, 
+            "input_connections": {
+                "inrep_0|input": {
+                    "id": 3, 
+                    "output_name": "outtab"
+                }, 
+                "inrep_1|input": {
+                    "id": 9, 
+                    "output_name": "data_collection"
+                }, 
+                "inrep_2|input": {
+                    "id": 10, 
+                    "output_name": "data_collection"
+                }, 
+                "inrep_4|input": {
+                    "id": 12, 
+                    "output_name": "stable"
+                }, 
+                "inrep_5|input": {
+                    "id": 13, 
+                    "output_name": "stable_sequencetable"
+                }
+            }, 
+            "inputs": [], 
+            "label": null, 
+            "name": "dada2: sequence counts", 
+            "outputs": [
+                {
+                    "name": "counts", 
+                    "type": "tabular"
+                }
+            ], 
+            "position": {
+                "left": 2616, 
+                "top": 320
+            }, 
+            "post_job_actions": {
+                "HideDatasetActioncounts": {
+                    "action_arguments": {}, 
+                    "action_type": "HideDatasetAction", 
+                    "output_name": "counts"
+                }
+            }, 
+            "tool_id": "dada2_seqCounts", 
+            "tool_state": "{\"__page__\": null, \"__rerun_remap_job_id__\": null, \"inrep\": \"[{\\\"__index__\\\": 0, \\\"input\\\": {\\\"__class__\\\": \\\"ConnectedValue\\\"}, \\\"name\\\": \\\"filt\\\"}, {\\\"__index__\\\": 1, \\\"input\\\": {\\\"__class__\\\": \\\"ConnectedValue\\\"}, \\\"name\\\": \\\"denoised fwd\\\"}, {\\\"__index__\\\": 2, \\\"input\\\": {\\\"__class__\\\": \\\"ConnectedValue\\\"}, \\\"name\\\": \\\"denoised rev\\\"}, {\\\"__index__\\\": 3, \\\"input\\\": {\\\"__class__\\\": \\\"ConnectedValue\\\"}, \\\"name\\\": \\\"merged\\\"}, {\\\"__index__\\\": 4, \\\"input\\\": {\\\"__class__\\\": \\\"ConnectedValue\\\"}, \\\"name\\\": \\\"tabled\\\"}, {\\\"__index__\\\": 5, \\\"input\\\": {\\\"__class__\\\": \\\"ConnectedValue\\\"}, \\\"name\\\": \\\"nochim\\\"}]\", \"chromInfo\": \"\\\"/home/berntm/projects/galaxy/tool-data/shared/ucsc/chrom/?.len\\\"\"}", 
+            "tool_version": "1.12+galaxy0", 
+            "type": "tool", 
+            "uuid": "cbdb7105-6e81-418e-98fb-7f36df117fb5", 
+            "workflow_outputs": []
+        }, 
+        "15": {
+            "annotation": "", 
+            "content_id": "dada2_assignTaxonomyAddspecies", 
+            "errors": null, 
+            "id": 15, 
+            "input_connections": {
+                "seqs": {
+                    "id": 13, 
+                    "output_name": "stable_sequencetable"
+                }
+            }, 
+            "inputs": [], 
+            "label": null, 
+            "name": "dada2: assignTaxonomy and addSpecies", 
+            "outputs": [
+                {
+                    "name": "output", 
+                    "type": "tabular"
+                }
+            ], 
+            "position": {
+                "left": 2623, 
+                "top": 688
+            }, 
+            "post_job_actions": {
+                "HideDatasetActionoutput": {
+                    "action_arguments": {}, 
+                    "action_type": "HideDatasetAction", 
+                    "output_name": "output"
+                }
+            }, 
+            "tool_id": "dada2_assignTaxonomyAddspecies", 
+            "tool_state": "{\"__page__\": null, \"tryRC\": \"\\\"false\\\"\", \"__rerun_remap_job_id__\": null, \"addSpecies_cond\": \"{\\\"__current_case__\\\": 1, \\\"addSpecies_select\\\": \\\"TRUE\\\", \\\"allowMultiple_cond\\\": {\\\"__current_case__\\\": 0, \\\"allowMultiple\\\": \\\"FALSE\\\"}, \\\"speciesreference_cond\\\": {\\\"__current_case__\\\": 0, \\\"speciesrefFasta\\\": \\\"test_buildid\\\", \\\"speciesreference_select\\\": \\\"builtin\\\"}, \\\"tryRC\\\": \\\"false\\\"}\", \"reference_cond\": \"{\\\"__current_case__\\\": 0, \\\"refFasta\\\": \\\"test_buildid\\\", \\\"reference_select\\\": \\\"builtin\\\"}\", \"outputBootstraps\": \"\\\"false\\\"\", \"minBoot\": \"\\\"50\\\"\", \"seqs\": \"{\\\"__class__\\\": \\\"ConnectedValue\\\"}\", \"chromInfo\": \"\\\"/home/berntm/projects/galaxy/tool-data/shared/ucsc/chrom/?.len\\\"\"}", 
+            "tool_version": "1.12+galaxy0", 
+            "type": "tool", 
+            "uuid": "babc7594-ae73-4818-919f-561836c45c17", 
+            "workflow_outputs": []
+        }
+    }, 
+    "tags": [], 
+    "uuid": "a4ba74bb-5a8a-403d-885c-822bbe9d6e03", 
+    "version": 1
+}
\ No newline at end of file