Mercurial > repos > iuc > necat

>&,&*(,-;,/-7@7)*+-+,&.2,:225AA=;=;4C462:8@.$,0B/:A/3B<8IF<1-/*,6@65;34-/A91113E50)+*1CC54<B@C=D0+-;-/%&&$$%$&**;:;6/)1),'/353%(%'%')21>4;2,(+)&&&&)*++%&+,6:8<:)BA?@3333020+/<F=&'25,,,*7>)*&1,*++,167><@76670/,)120(*+&398=39BBF>9;0/%*2-2*7664/)%1*202?3+/7/0-.8;:B3BC<<>4>3+12))(*++;:4,6*)000?00,DA00DD<85'+.*3*(0/4*BA18('&/8;D/6+<01-16>C+),7.<DC+64'1&)%'$'&.-4<D:7E69/%)*1;..474:42/5=*:9:3>2=2+/5+B+.0ED:4<6(5(1,-*%&.-55=?:;B/''%$''**%87.129@>65./5--4101'%%)(*,.12-/45/,+,)+8=8:23695?8,.3>6@2-*2-,(8:*+69FCF=7871644A5194,-//-2)6486<80241<+,')66.095+)4-17<=78/,--/?/101,+)'3,(+3=()7;,008?D:,,,-,:F12HH@<GF4<;79&3&'&%$$&&$#+)5>.*0444470$$%'(*/3,636496))$'#&&&+%&-*+668%-*'+-.,,,579D;<;<H;98C280*050:6254';88FG62EHA156=9<=96861/.,&'/*0.5?82'*054,)+)(35?>?C,+)(-9-,,.,:,-,1:2/1003B'-4/:=H89034-'+&.),6&228:7(:(4?+;679/1.90:DA':;9?860'55B945642763(-3(*&$&%(&--))2**-&9GGHIF?B36-1**&=/(+<81/-1+44-/%+04AG7683B2+11G64%/,/4*&&-5137;$'$&%&(&*-=>C2H42.*3-),,3;6<97553=@;>@0//)#'$(%'CGD>,/+%.,**'().%)')'('*,($)%%''&*'+'(,..0.(-71743..2+3./+&'.++7'+7/6775(2-6-<3'$(&%+'$.,-*/99/52.'+.7680,&3-&1,.'')++*&4.6889;CD:1EG72,/:89>6278,/)(((062112857)2969001.+)+,/57E>*976,.60(/2,*,((12/559:?;;???@$*&$&)'&')*/%)*&&(&*--712+-*01-*8+,=00<=,38/.3.(+0-*.','58*:4600,-,((8BF-*/,,106?12A7%/9-038<@3100/0.3/64>4''+'&/,,,/2'8AG8AI4800-2')'*8-(:-).2/'(+0038366:==>6F52374%))+5A=379313('-2%+.),2,71&4;/1;72/0;80&%%%)1*9*()-+/,-)+'+-/*,*-/:;-=-.%(+-@E:588(%64),ABE>=>E+,11),09)+,<4;)..&--/)**7<8;9:+).12:12,()):-,'1;9AG?5*0/,.++,0<?B=1)+1/.//,.0:+88=??==D'2'(-+0+,(+--,+.,;=B/,0344/11/1=430GG3).258313&.?*)''$'(*16/:=07<E:4(),772236A7C8-7038,+//'%&'(60('-)0))+0-.*/5D9>D246,&-&'',-35:02104-<A;/546;001FA1C3=5@2/224C=BA0+,'/@403@A0&9*)(%'(--*-.1G,00/1/@=8A>639E95F0/'>C?FD65.1&3.(/&&''+04:1,*-)(,1<@740+-+-.0;55=3.,-34/+,00*-..4/?6/(',''%(0,&,2/00-((,.:2465,18:7--43<:,+656@8<;@@D3D.4'*+35-;;3;;;&95((-/''+1,.(78CC112B??;=C6'%(,/>10//11/BHB-+'&#(3<A8?)7254>C96IH4;*3-?>431518B6.0/48/<):AD4/))&03*1.-/9-;;;,%0+.16656II9:9>-/93;-)((&)&+8C@5=21-+*+)19886@D>8720:5,-2857;<<AHI=8,0,6.90.3-/0:7;IH:,(&&(+((-4599,*5;--3321>=,14-.283?CF2001('5).0D<5:78?.)'%'&-3<58:@?()/'(**,9?2,/26-(/2(0ECBFA8>57788,/9?=7&>(+'0*<DGH>-/759>HIJF2-/18HE>.84/+,57A'50.-6/3E32*''0:158389B=;;89:0230*14-+'')2)0%6>4/8-*5((-40:8:6=;855.3,+,/1=F>A@2+,44?7,3=@,*,317B431154E618:8,7<,),($*(%..2311564+-+00.,((+,$,%*//?7DE99.,,,//+.0,/68,.74:;6;786:*%'%*.)00=;5*-.27269-/4;806.-1622(56*100;78835.2,,-;:3:?@0))(.20,**3*34+-0<5,19+64;3:0;26;IB8B123;FA21,.29<@38)%+((+.4(',*+*+'0(,(+'99+*(*0(.45?/=/A1B//2214A<35@@;<?722B5?4635488;/*+*1=BB>7/8//)0,*%)7'30.?54*($&)%'+./,.1=>/'230<--+441:13,)66&1(*1(/2%''/.8:7./*042=?.*.1667:?D?41,6-,,0/10.2))04.9B:;>35;FB?;..0,*,'/78@51,,&*+AB<>94%/6)$.(((&((+-;>,%(%,+0;?>H@A9,0/41:FA+124//-.22,@8<0(0+0:B036<55::956@9118>1;47-2*,)*),3A3347//57315425:BA1772.4=,&+&+'+-*+&).34;471)('*,$)2776$+')')(&&&+--(-(+1-+/3;>1-%.//7+2A00*>GG7.3*5D.38@F;9;74/3440.+/=0*-).%*,%-'9?@@5577/+'9?,*'('&));-++).(,++'&*(/)'(*&*6>D-&()-/00-<7=83@E1885221/()'*(((*&&-4.022;?EB))(2.+)%.+.,)&%$)DC>FA<//..1-7E33?9@=4245-///+('&%$()-.-4==@?@A4;50)-,)*276<),+20=306;7523?*.''%%(*.*--3,,%@,D;DG64/3:6AACA3C0%*03249@5374126@::87;40(5123/(,++.26;9;6;HHH=078=@18<8<D;61.<-/8.;;>,)($&65AAH@668,46:>61,-)+5>D*25.*+,11052<1D-6&&'-126<B*''4,1-<8:8:+37858A))6-@<:+/7-52,1,5-3ADAJ<@*-9:889885)92:04-//01787;/,--5;-1@,(''*)9C</%)*.0.1,00/47:>=?8456:88>-/-3*&(*''(6:0*--08FA9=I831,.268C22#'+658E<111177<C@32$###$).2;=9@?==>B@;G:9355440/%2.04A66./38469285D=H297056602).40*2).047+)&*++/(1+,),2--'(''('))*-<(8+3.)&*,*+/25415..(&/*).2;6,0.50:45F:35/')2+/241>@9<@C69+4&&*/-5.,0*)&<8,,/264==<-:-'*96-28)33->02==257<9<+,5(*&)()*/16511=7-/')+'2+2..55,-&'&,%07<>::/),(&&+,1*+.2DC<A6@<1%,12548/0*3,:-+0/*.77:2666203))*-0?/92,;6.0$&(+-()+;=E96878B=.*./,<90/622/+.&..;2309<.).05A?8-)*-0@(*47051(+++**'112/++.3<8=;H(7/91FGE.BH>;==7*'+-,/00.=</-3/((77E<*6,02,*)*)+),)(),11GF7123*(&'*'*(*'*&''57;56;8>31323?<@7..&)&'+3/7.,)-*&(1;</:('.@=D-*..--?G6.>CCA981682>=40/,-146'1<+8,/1.+-*.15@3-0++,221A2BID8?@+081,*--('*()('42),&('&%#'%%'%,'+)'*'((,.)-',-3=&$##&*)935333=)+(%#,')('+(%(&((*$)'($*''&)***3,+1,1.79;9=1.'&&'$($))1.1316-02A60)-3-+)&%')'&*+.(*((($$$(&3:8+0(*./-5/-.1+*(*+%.'/2400+&%,,)/.-&,-2-&,0'-(77.%'&&(%)$(*13)+&7//.()+(++','*#(+&,40*#&*(+(/)7<;600)'/62?/&$$*.+,)'*'&()*$+*61*.)61)''.-)&&$($-,*3,69*%%0.+&*&$&&$)++)%'(22&4001(5>781(-2/B1?31*)+))'''(&*-.'**%.$70>965:,*''&%)+(()*-8@53)0,%%$$)'))*,2418800*-'02000/=0/*,+5-*'$,+/@@@,/',)(02)+(+*(,+((')$$++)1//45'*,,+-'-1423(13*+)'"%%65;:((%#$#%%&'++(*(,',.,%*)'---/29+,%'$+,.=64.+.'*%)(&-2)),*2.(&*&)*,+/0(,.$$$*$%$%$%(%'&)),;02163/28342..''$&$%(%,(',825,,)(+&)+&**+-*01--/-/'(&*+')%*),0)0.(+)(**()**&%((%*/2,,&-+0/())*(953,,+'1).)%)'&%)(*(/-(4;C62((&&.2(&(''$)+('1845.,%*'%()*((++*)/0<*&("%/+/'0+-,1+)(**&'').79/7,*-%)%*-2)*+&&*+&33)')'%%&$('/181/--,$,(%((&-+1(''')-:=.12--@6-'>5)</,)$+*)/'-.>&/1**.35=6.',(.12(1?0,/-7?8/&#%)$%,+''*+*3/&&&&(.))+4*'+'1<29:,'((*+,*1,+(*()+,.$(%(')&)('%-0((%(&-,0.)/.3-'('(-4**%)'',1*0,((#(+3302/-22-32(-(#(%')(//')*-+*)('(,,+*'),'-:.*&(%&+.-)%)--%/,.+4=.'%*'-,.'.+4+/''(/8+(30.(+*,/**3.-.(%,-**(-./''.)&#'%+((#'('(1'&&$(,%&2@8+)$'*1**''('')&**+%/-1*$'#)-+6($&&&.,'%)&&$#$+/($'&0(-*),/(+$(.+)'(+'*&)((+++-,'.-.,-,')))(%%'%')*$&&%'/80**&1&(+('(%())*)%&)#%'*,%'&+*%)'(02),')%.*(')'('*)'*&')+,).+(1*+,6/+)#%$*-)'&#'#'(%*%($&($%*&&(1(82&)'&#()'-*')&(--84/6%,$$&))+)$)&$'%-''%%&+.--4//0('&&39.'*--)*&(&%$+#<**)('&&+,0+'+++060*05)-954*$*&%'&($%%$&)'&$$%%$%%%%&,*)(%%&$)-*-')&'')+%(%&'(()%'''$('-+0/++(('&901'*&%(+'%,*+''--)('&'-48&)%&(/11&1),,7,*()*+06/((%)$&$%#*/)$&$$$$$$$0-0('+14((%$&%%*3*&.620+&&)&(&+*'('**&)+/',-/'+),%&&%*8**()'%0<('$%&%)%''(-*6%&%$&.,.-'85.*'+)#$$&('-.&()+2+()%)*-/(+2*(('(+..&'.3-2/9/'+($+&&(*0,-0,)21+)'*1*$(,-&$&&+/3304=.(*8/*221177,'&-&'21'*''.03+((/,.969+>*%,)'(''))+*)''$-(362&&.7)$&)'12*(-&*,0,&*&&,'>5/1$'$'(+*,(%344-(&),/,*'$&$#$%%'(&)+''&&+4*+)%&(&)(&*(.')(,/2..0/**'*)$/*$%$%')+,(*&'(%&+,(%('))%$-()*(%%('((&*51/5,,'$$(%%&&%+,()&$()+*9)'$%$'$&&'(*2))&'$-03/'0-($$)(()'&&$$(&,).(),%%%$&&'&)+.*(''%#&)'&%*(*&%%$#&%.0-)(+(0*()'%*(('%'$%'&%'*,)'*&,+&%&$%''&('&'((-*+*'',-8''+&%((+/&%'3+&&'&%%'./,%(%%'%'($'%'($&*'(%$''$,)&&+&(&((2-.%)*'$$('$$(($**$(&#"((#)($('$'(#"&#(-%(%'&$#'-%)"&%&#-%%$"%&$#%$#:6'''$#####$$$#$%%%)&)
@SRR7477814.1.16535 8158684-b1f8-4269-997d-304589b421d5_Basecall_1D length=2489
>$%%*&.+0349*,.>--6/&-540951/4236:2@00,2672++.C39=(8665JC3411,04/25,3+22$--+0-.,*-+51/.*)($&#('&)&3*)*30-+,/9-010--0<42....1/-6:5:-/5623D8666135986-2408*)&#)*))%+,32?,+%&+,''('.3F);,77.0B>0*)*(*544>/--(61:>124?;6/=3.5:&+2/+2.((=74:B;;C702>3-3;<2+.)-%-&+.()74<12.+16158,()+-)()--67F.7;631-(-2F<C597*,&%'&42423*/,&)%,,0.-**/6:.*+4,1?52:=FFE-30.3AC<01+,44=409244A828A3/-/023:25/63*0)-4=8C?5345655'')((/13.(*,,-83=9@D>//-.),).10-/0/9=:.,./5E@@?<0,''&)*)&))%#"%%*085)04/+3)(/,3<132)25*=()&)/+.73211;;025<,%(&'+;A94,'((),03-135:=:@0?,/'(&$0*-&()-28C;<<57+-1@6G::C.,.6/9GA843D3.+13<7+,4742CCA577)2692-67:97414CGE,((%..<05.-04:44126@=290'4.-,3/-50046,7C559/)*(,37A9;GE?>?==:8820+.77?><<'9.5/@3/3*+*,-20,33,,/5237D>;<;'4,/4:;2HHH7A@B?3C/2D5.2*/<F9-;97..0,-))>6+/64436112*)5/+'*-,-)1*..-()))-.*-2<D6FHH5;BBI?=C:*3.*>?<167.(&(*DF3,-%%-*);-+89-+/:5.56*+-/89,'&004FAEE07,0*.2<E20/47-0;HJI:078667401037<29*'('-?5*&,,)8E4.*2,106//%*.4.1696<9+*3--,(2/86*+)*2<784-&-&&')%-&&%46;A</..)-0,(,2,)23@<<<9+40/,27>,,.+&+-/;:.<.43G55+6+,++&&%%&3-)&')-07.66/+7B:(#$&&&&-+->2?<7/,0>HA+80-.<<8715312-)&+$&&/0822(-2..)($%#&*+131,0;?*@CC2112--%+'+*1/,0?,)/5.024/2;'.(/.(,0-32113,+')*&*',-=*2-4**'*34+*0/3(/5067976./2A?3.7)(>.043:3;??<<780.+:-%-2-&5&$$,()*?,8/78D2GJA.D=/%)&(',5840&&*('((-*602-:*()$'%-*7CF..,+%&'&2287/>('()(,,/008:4/-(8-%&%''*)'&-++,',,,+4.48/24FE03=1-320:7?2/1.+.46.-/&)$(%0*)>+()$$$5+)+&*)*/+',(-*-8101:,***+-<6+.&'('(&)&,0:H:8?=@6?3597:83.006;)0-)(.,)9//91:D?/4/(&)3H:A6?775>887<>/021<G.@AEA=A1>1/4.-+.0/2DE<;72*&-0-2.$''0*104?BC?>642'+,21214/6=9/1699<;;,.)+94659;/*('$'(.:41,-04,'&.''**)+*0+%)+*(..7>6+:--(,-2)),..A799()'&&*(*'('*896*3>7*0,++-,/6./))-.567>1<,--4(',,(.91?=<2'/,(-'455-&'%())09..5??7-7''.(*:2**,60+;A8897784-0--'//'+.36B?9=>A7;*1+116.0121*,10/.12(0'((07>/+/./2'4/2351217D;5456?87888:7:.0-.&12,*''%)&*00/.;*-202135@@1,*,)'33446CC>2=<A010>=BB11+9./AH?898073,(-.8324B==@;8659939:)14;0/57(+*34:FC9EF3204.:.-70B3GF>>C85)++()(-*$&*8.;8-,$$$/,7C2>11.@A;8/&*-.79CJ>3=>?EECCE@')/))%152@7>??+0()('-:6/((,06@A<37*',+50:9==D:9<3-(,-,221&'+=<ECC780013'&&/)*:A=A6:9GH84EF59)')+550'')2-0=689@:<:*+7514&&)()48EA)(*-).8=.0=3110+&2457:<958;43/,32551+2282-(3)/,,&360,51?1,*.')-3/-0'2@@D2@30/,240+79A;5;/+,.794///2*11.5.()('(1/0(.<8A@84774:2-((()*,7959<=946967;77/-+&&',*13-45-<14,(..,,,-1..8?;8()29;5?89/2*2/23=FDC/0,&&'()-+.*&))0&)'**,.+.,+--00*&)+--.*+)9))(*-62/--./2589:6?DF058/+3H:G976?<10*)167DB<0416</=.)(*)*,.33-,-+07>:87:4)'&$#%.$8',/6:GF8<=(.&%'%&)/-(+)*+:),*::6%.-)--)-55022-''573>0;+*),(&%&$
@SRR7477814.1.35442 413f9b4-29b2-4fa8-af12-56872ab3856a_Basecall_1D length=5492
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
@SRR7477814.1.21086 c85996a-ca90-4868-ab26-038d882d5075_Basecall_1D length=2161
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
@SRR7477814.1.14585 82fcccb-2269-4687-9972-8fc309a84034_Basecall_1D length=4917
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
@SRR7477814.1.6626 681e880-d785-4a57-8c19-6ea3dbbf8b54_Basecall_1D length=5542
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
@SRR7477814.1.44254 854ea28-ac1a-487b-b4e5-7d1052e33ab0_Basecall_1D length=5719
>'),(&*;?;>B9A&7.1),)0))52-+4(+).(),*,.,*(%'$*)%++(.12)-*+2A&-3.501984+)*+++,---/,9I@1=>77(35*.3;50?/5:8.76:=-(')&%'%)C>)8)2,24@;?96*,/-'40757<4=?C?5=<B5C5A37E2-157BIHDD7&&&%'+()B@D68660221002&'*5@:@2E121BA?B.0,''*28.<4?C-*)$')&*.2E94;.2003=<<770,3:-2=+''&(7FI3(&*-?6638;5??9.)()/,461609*'+3101)6;C>D*=GB?A<097%()/15>58)%'+/594107B/()-384386C4/G0/19?78CF1'<+2101>&(').3127-,--651;53;10/,-05'$&-.10)+&(,++*06531201*23><9:>A.*&'$*+&))81/7DIF64@86=F*77662.)2+347I?9/E&;))'&&%%18<--9H2610/(+*)&+)+(+92+,1939+0=B::A24A17B*DD*($')(B21+,(')2/6-6177;B@C6;KJ494=185<EC/.6F2..2238?@3+62,B.<+'-(,48/..()$'%9/1-*00*59:B<:234).+,--,7;(*(5+3,.3=837,,'%@??>11+%%&%1-101115=7987&'.#)-**+9809(&-874.5,$$%--+.24<6/3/))*&)&6)<2,2*5*56*/;<8HA61++,-0&+&(*%&+(.05076*%4<-.402*()/20.+,-///-/(1/5,02G9-<=9:57%)%(%&')%'(',,*/2@;4)0*-&%)&$',(87()&375?55E::))-'+/323/,/;9?6-)''(*)0'#)%0(+.*0./6576927943/;::</(''**&)5025:;E<8002*,/62=1('-87/*0,.19@/46+*+)%//)+)+143;5/2:4+9;2/:+8<6.0(+060@A93>F;:;*;7G8203135:321/)1321)+',/9801=?/3)%.-1>222,*)*)1/,,,'&'.+/011014625<5B4)*-.-))'9:2@.4/=/119?:46/.0275/12+78>14C1.-23E<21-.*/5*G=,)>9=*+<-0<+4.012,(&(&+,)***&./+5;-C843-51.<2**)'+)+.219+3633+8:-'65972/5+)+',80;1,/+*,/148CE)@6(470.7>>E3020)+&)-,(-/C@;B0A/01<;9,,%++-010,)'&)%734+9<;6568110/:8$%%+2)/-,*7@E43BA,02.22*))*+-'*-)(%$''-*../(((%%',;()'(*&**/&5><2**%++..(,++*1@D6'-(&#)(//34@248;:87.,),))0064+*&%'((.65359736),/0);490.-)*)..$4>2-0,.-+'*((&**)+26719:;99A/-080,-:4.:312777?/*)*&+,19>791-*)(/203B2077632615..$)&)'+,>-;/%(-0220.,0+4/:81+1285?9)-%$(--*',)(10486:)0+76(%&&''/&),*)+,-5324B645<:86:43;9>.$$17*AB;54))'1.))&&6;80/G>7/D*23807;;<E0<?5(..<00::>1-1;5=99294,08@BD@2G0.3.11+16&4-*+&)2(()+)9@)*C@9=6/4812272722*+-)--23..+''((&(+-<7,'076<5@6>6106*,-677453'*+$)'%)+*,&-5:;234,/*32,-*()2.,.+---003448,C;>1%)'+%'(437:'5*.,-2F>@EB7361/5787B57=9674963&,.248?>@@A.8-**-1**(+1---480*%$%%&/60--/%*''+,7@FEE;-.)*/83=G@EBBBAAFGB<C:,/1./&*.1-0//*//(**++*'$$1258++.6/0///0230*..'('(&*1B1/12**((%0401>7F>*+GHCEA7,1+)++600'--47--.,,+,./4+2+**6:=32'*1147C.;<*)2)/-39:98-)*+,>-,('*.4?+/.9&&.-+77/0+&)05C@;302<7:=-*'&(,,)06=A5BF,+5-65&&21=B9:7-3=E?=25,.-6/;56=14)*(+)@)*.,,/-.</<@312C367667G767B30325;602':-+-+-,-.,+5?<7)2/057;DD?DA74,+%&#'749E>45:77*,*+(5,0,&5/+151.09213*5038?:''./4;<--*?B81293<8::<6544B>>>@:3.52/,*.*)*'./-04-0'&('*''%()2:<;43(-%.**),)(),29A323%+68135,-,,137,:...+12*'%%,+,131.,)'(+611,#)&*()031+0+0.=C@3*'*$&).04<,A62(%%)$22:)%''&1440,*0'%+//2;42/0048-;5@8/1465,4*$)%)++*/(,6',)/3465G@7*(,1342121%(-+*7?CF5=A3=-A,*C/.6//453-&'-')-67944,-2B88>B7C6977+,(*))*-,>7(--/2=5:45@?410100+0))(-?<;<@??6+,,,-+6+,'&(+.-(-)++.(/&)$&&+1*+&)../7?B>6463,&)7+2?EC9:3A//3/0/.),/0B:)9*50.)+-80;;31%-33117D9601/298=??FDD;=;28'))08(;H:1<F61//;>952.%(%()((+,3.>C0);.-+/(./.-44*234696=72..,))('$&)'++(&%'&'&,.-338/-)%0(')%(.)/*4>6235*.+*6IF1*)'++(4.+8.;;C<,01-5+'(('&7799931+/,.5A=+).10-0(%%$&('++962-+)%(*.)3)+),-(),*+*)'++((%0/.3)-4)1-6339GF5*&/*'/-0<>7=),,+%'2=-00-7/5.2-40+.B:9;(5?,,BE69A/-7607>9?@>/08+-+/,DB/,*$'+50A8<63+&(++,04:2('((4?>.*..3*1301/1111:8623,*-.533.%&-CD45/'(%*31GIB7CC8HHIGB807,/3FGGE25>524/)$%/:*889,5/0,,%&*26-0&$%0<?5HF582349<=5:41491**$(';@@-573/6-((224EC><927<76:=CC@4=<?/0.),')),2('&)%*)+-&))'9;3**(*+%2%*,---19>1,,.7)67..+&,9>0../.-*-/'($'+,73)&4'***1=/<A;@8:?:063C;*,5:..91F@H-47E9>78CGA9'4.%,/.9FF9::;IHE6=?D@?>762;>=5+14;994'583:<<?>>=C6)('"'+/5.+8-5;:5:,8))%(*:=.9F<665)694;31%***)143?<=8I76,)$&-563.56(--.0B541/'&'''258:C<(--+409@2+9)48IJA122,'1((.'.1<6)@E?8A.653-88/5133441/0/.413221.,78-74BA<D<62;A602<-(&'3=31731.0:.%/5.=B30:C66;=E4*5-),,6&*(%)*9/91569685.(()*)%()+*'&($*''&))5:.'.8.+6BA''&$&*1.((%%'',)/+.(()*3,*)*64--',(++(%'$1)-.1/')*..06,3A2,1+*-024:2,.&%%**3/5;AEBH01:1/1=;720-)-+),57+0,**%,&)--.1B>==7.&&(12))*/+0'$&(,)/3657656643*61*02?>1,,&,+*&53.9A./&*,1.,(39/))150-,+..,3A6..,/,2/2:5/6,2.&$&-.2--)*')*%&%&%(',.0+&/((%,020.67A6<<59/''(5928,-0*&*->>/+9/,<;1+/10.&4/0:4A@E739=C?A@??F>0>*;))>6<JH97C0-:.,35151*3*&)*)+;;.70).1/5379::4=/'*'&)+8E+:63101/2,/6:@6'':48>>4A202D1@*)&(%(&0'&(//.(.4+177*3A=.:?;ABD@C@E::7:'&027=-.-*&&'7/=?<(,0)''%&%-.-,-4.*:./20C<:=;87;1''),0,))*.+05*++$0-3<>6==BEE?87/0,1**/=H@8CB90$)*-787<101;=343.,0-./=G=><BABADACHCEIGEF9?<(09+.2DEE.68:B5@34?.120031/(*+)'*')(-4A.+015DGF9/95995946/8-,22-&%.79@;.%(*).4.)+*'*)2/,4042,2*)/419461162.452/1)()3212-;10AAB,5+0203/;3?3')&%#%)0*&%)+.=8:;;87531310,/4->('5)4(&)((6687===D3/.++,,*-*0/8F277?3/0**).&**.))-.16=0;?3:974,.+(0,@-4,-'&887<??B=F:A4B=>FFB?>=7/0)-&&'9<:25,,+41,3647@<:A:11/0+/39F@BBB=9($*&')*++.4?>A7751.((++228/+8+.3/+&(%'',23&')37+(.478.5(2=..51*1/,,0/6,6<?<@E?7:972287.%**0-1%&&*/2;5-294-3;<31268=2.0?,?-&-*,2@1H835=11''1-)#$,),(.*/49=5=4082*&%&(,848:B8<A4CE14=4'((,&$3+72+-&+.01/))$$%('1-98<GD6A,*,,+&(.*)1*)')($$(/9+/94,&/0(#$)*..9E56597272#/(')$$%).25:=;..)-.--//('%((0279:24&()+0428242/(.1/-:2G4:493*%(,,95>910,*'418B/-30066)==B=CCE+*+)(%-***041:BA;A=@7F@EFEBB>-6--/23&-.71=<?@:;>>')$)*6-<?+5.,-441(+((*2))65450;467<=840%%%%$&)%)2/.)&.2;*.$'*./0,*.--+01348/9+''&'''')*1.)--&++/*4D>358395101/0*7@??>8G:<=01',.2&+-,%$$052),+1(&&)(+(0704431*&)(/*/(6643;?@@FA,-.//:20,*//142,.+-+%#$&*:,=/+.?A;G;<;<*-1=>@4.6.,<F>96-0+++,0>?A6942596026;5(,,6-751.0/*)**)2/67645021').3-./C5<15B55.1*1:7<;;0*)*-$*%'(1;;?8BB?AF<6:200.0&()',01/,5>=<:0172@:4.%.0+0606255>/42*--14,++6/661(+(&%*,0)'028-:-*.-)(,$--=9.//7-'++--4=B5310%**-5?<1+,*-3-5/9'/(2,1/,011/0+(*)**1254B3>H88.07>D?5(314.206'$&,+22-2.,,3579.206/'*&""))8160958;;BD@;>-:.2/1.-4.--./DEE6*22-4/**,/+03253)445:EIDEDB?=B?<868?/0;7BEGF2F@/5D?<=7%$(%%&#&$-,)0**-)061+*,/'++(((%&(,46ED10/,0=798C81?B;;;6;=3?5??-6G?87@426028:03,7+%#%*-+23.182351/*+*'/(*(?5%8'$(6A;938.95E>>1//2485,.-))032).-.<D5,7/4-2656;575;).%),-6/.*,01765**6.5B8A27EFD<I<89C22+'*+>8?7FGG7:@CHHEI==?D@.-))+,2,@;:4%'''%)%',+,EA7,-*$##
@SRR7477814.1.32019 bba11d8-fb42-4f92-8bd9-4e0c23de3fc4_Basecall_1D length=2579
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
@SRR7477814.1.7697 9396946-0c96-4862-b6bd-6e999ce587e2_Basecall_1D length=17892
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
@SRR7477814.1.42848 634d2ae-6e7e-46ab-95f4-4557c3f6075e_Basecall_1D length=5100
>&$%-**.8A944@=&2-),823()$,&)673<21/>+*''&)1:(4)&<A6<F4+'+((&-23(#+4,,))<6/.00,.2/,8HA5@DAE<7?*'(,*.*($52)(%(,.*-.617.0,(/:B+%(&(,)/1/86-.67221-**-(+%13..150028..11-$$$(/5650,1,((001/0++,(')*.(.))*'2(:<<<;38@9205646;9<03:;//-'-.&1''(H/+)5(4)477=664>,$+%.%)+.,05,+5@3300.1006461(),-))$&)0+--/96:38-=77)137=1(483>=C-02),@@?CBB@18++/$531-3.@G8C=9-+-1125?/0B/@899965.*+--19-48A5;F>>?88@:2.'**,02C,,*G-.330+CBGH7?=65@?HG;9'4,+,*-'('&)6-,2,%()(15509?:<40./;4/63E:57CFED>5A+(%((%'+659?6E>6035>=6--1789;<G.)-:.99D:@?65:*'-34:<BHBA=1<>@G8::976:898GCBAD3:+**./7;A<48+.)19AA*=<?<@680+*+'5<;BCDF769;*;A4(%+,414/*,'+/214+)3++47?B=92122.1045'#)-;>?@AADFE2ED(#%'#.0=@>34)9589DCF>DBCE@7778?96,)7;CEFEGE/D.=59;@B44095000),0)')%)%/+0,3;<.40,.*(+-//DHB:9:/99639=256:74..&6<76-:91),0:AD@246527=?-,+D-2270125@4)20.-(-)4/12C?040*+*.006..)()*')*+1)-5,.123=/0*-+0*,-::B@@5/0,4--/>??D@..?DC@14()&&''),,27@B9=.)&%+/-'28=>D;950.157HGA@;1((+(.25?8,51/1)('/01H<<<70/0:B?:8/,+/0%--*-1=/0/639?7541=/24@<24146+)64.(+)(#-.)4*,0+()6+/*34,9C.6D1,23==.747:+-=(29(''&02.--+-%++,5=/,%,.1+(*,&$&'(('%%'41(,*,*85--.,-))&%%'(21467;82355+1103)'),,6)%'-88@J>=?24/0.5$&,,-,%&(&4512/+,/%)+&''1.0?:8(*4-175=51-><8?,,20676531.2678:,*'*'$$%$%.(&&2,=?82-'''&&*00(*02/'+1,((&)#$$$&,*,10:-/+.0--0+(+-/'011+,3-&)&(&%'0;;12333BC5C712<81),''#6C993152.3,,--.$'/+//'+'=5?-%%%$'''+1<48<>:,,(('658976*)5@A4=9D74=:@56-1=?7E7G2,--/1B88D69;-5/,43:.&*,.B556DEFDDECED?BABF:;,/**(.+A--++..5'3&:():=<8<5')'1595<;428-1794-56C7354109.14.377(9&$&,,()89:A<<9*,/;4;@C?6''*.5@=><1<+3-.156A-)+)&'*.-5A6)-/35A.H)C*7/,,+)('%*%$%3+/<CA7<<;2-*5+8D.7;7B=@A@8/79:;CB?+010.,(+,7958;G:88A7=;?898@2)/11:.+,-,7A><6)',.-2..7F3;AAG8/,*+7==@D,AD.+,-(:::70152+/'&+&)2@D79@E=:81:7@G;;9;7=EF@2/)14:;7439*1.>:E@43>,),*/446*.,124+,*&'-/*),*-0.,2.2384?AG<90:2910.7;0-:?/161,0-,*54(,)&.523=>BBB08B@C<>HHC+('),6.A>DA24E3.A<;34-/),+-3BD/C-,643,,/45<@>=6.:))'&).+9@>)64;;=.09%(++:?>+123FIJC86<>2B@-('%&2BA?5/@:<<D2B;@%)6;<5*2)4);.=3$47+.90*/2')*'78782(+/3:8:<59(((3<8-0+'(''*-9E?3907(/A>29:<>@EF=8:;:B@@>:9=5.;GC;.)+19911/+*6@2>?D=BE,B+A/=+33,$./..4>?07:6/056<JIG62-*2-*-,(3E:F?44&3(3.77>@/11846=.4-9:E=266B8>;7;,.<-DGI-/;=+1))+.+-.-/3GF0<437430(('33==D@>IIG.-.7>A0DG1/DFDAACE=487,4*(+517:<>,2B747=1)*-5;(58DEBE/-,+,-;:;55.&)$%$&19723120221/()1322,+-305;<3?'/,,597-/+/,41*:5*(3259EIJ?7..*+)--10?<)=E9?9'./()4;<19:4BB5>302933300@@?>>/.'''1<))2*0-,6?9<D778<7C@.1210..6-)46.03.)+:4256>;ED?98:59:7::;;5;B92587.,9EF8+1285.2+,*559;@D(5,5+,2.'(IG2*,0+.064'1&'())('',45840585656=.)*(-2:4+&--/63464466/2)',10@117<:17/<<5E443;-2+1*,/>=?<=97-11,&-682435081;59:.2*-,,-965/4./16G?J2/,4)252**0<7386;<F>@D@A<:42244A88)-0+28,(',/0/4F@<;:58/'&%*'5@F31<D/5--.-)<E1++()',476808-7*&(/1,/(,),-/.1;,38:E7,)7+.)&/?FFDEI>+1-)3?.4*;+4/('&'%'@4.,.@;/62108??A403/27H<=<H?>))-+)%&+</E-080AJJ.C@.40-.,1/2;368,:.:/,-778,:)+-<:?26DJ;5B?<<<@?=AHH6F+15=/@?:D9;;::7559:=<:;>;<E?8=E9+75570.)))4@=?.1*,,-26***09:5/./0@?<=A9;7($4056''(.)))9A64.'(+@.6E8EEIC76689A=5B<@B>AAB>C38/53GGD;689655A/1'(39;C<9<7+.,/;8=E7B<9=F>8;7530>556C<<:=@?)/')8,.+.2>::889::>4./-)01,;<,''+,7CH5CGD=<<402/,,54=5F;)3:7>CB52//744/507847(,-,6JA5(*3&((*.0?130012EF531@/4,5+@C/.;H2G23B713356.30-(*%&%')-8')/22E64HEC8&62:/25B?8@,+/6?69:>67+%+-/+)**'(14526A2>//0526:3.*,-4D@47A0)')%,:?8<>A<?;E;799-9:,7''&,'683&'(*&&%&'&&+--1:3<7798153(,+,/678:<C@HDBCC@:34.:1;=:AE@:*+,2.+,(-/0.-0)*(++:08C10)1-+-<8567=A=+*+=1.+,(0*-'%$&(/=-+'&)-*.768=C9C>)**)-164383.-.3/154?*620*,*-)*,-6-.=/.F?/)(0088<30?.066F33002-('+144A6E3A00::/;@5,,0=72677513.4.?<.8;')*2-)/-4(>,7G3200&'.04>-,'-&+*)&+&.+-1=H2?:0.%3596AGH-+'+15132783.+-03=84,,;F=>;874'')161.,+')-6?@5B*)*+,0*5*'+*.-,,($/(**&4DC5?-)/1,72''12=B37=476;E>5250067>9@?>AC969:AC?3.3243335CGBCBBB>@C6794:7448*+2-(&,)=.F33)''4F:BADB@@<7.&$$&-42/,(1/+60$)/=01,0DFEGF@==;3-2<,G68?HD:0/5C98?:;>ADCAB@A>GB7..2-.4:9521+*.0924603323>83:2---+*+*&-,5683A?AAA';<>?=A84/))(4/138401.'-'*%()'225989?BB+*','%')+.996.2)0(..30+4;:>80/.,1*-097;H?:=D69.0+4E11/*-54<505334<26D502332;B;A>@4.:,,-4:@444<9689;C??AA@IFD?>===AC383'3--,065>*/0FG9.2?*00',4,&+'%%**53)1/676=6;22/52+&&)*+000+</-/4/*'))7E><7@7B60618'50?35<:100/))+-),C961B:45AG;8@>D/12>7'.2'*((.)((44'4277=/@.)1.@;08,'(++*()&)9<GEE:7/*12<D-)32*4-(&+02@<;@;87108;<4&''*.95*,(&%*)+'1')-++*'*+'+/50)'(&()(%$,/6;<//714*()*-.(/83)9.-4-,+&'.':3/,'%.?6==D5*/:,6;/+++)41-98,*++?/07860305+6221/'*+;72&/-()(+,'-/439A6>3@@2--,&7%).$'&%(&+1<B</36(*+,-5*,)%&('/*()*%(%.-/:(./+(%'(+);=2752.4<)-'$'.+&**>==A?.:($)')38::04.61;462,1;;?A@@=4////046/'8:,/:888C4',./>://1/*/.025164/014299;83351;.5>20%10(,12FG;<:13-0(10+*2-'3B0DC665526//3.>;3-11/27<0/-2+1)/+/.*+6785-%((')%#',-(,,.)+3<913ECF=05-11?0?>>576597;>D5337654:67/')),+556C@?>I@3=@4:3,+.33>F124C2,037BCH41/((+)*(((:0=;68788G@?>81>/).0-07<8994<((9D5:<>1EF;246;8@C6->222FECCC>GDADDFG?3,1@B879?95)<6BA:=;9A:E-8--/@23A@=2;BG.7?@A=@B1:-706653.'*(1@>C/EDB3121347==DB&6CA9AA@A=/2-9<BE>:64892,0.5>//8167)('%)%,-.C8:C<7.&&$+5*,'&'0*3,)&++./;;+.@/6CDCB0345@:95G=666,*)()%&;25@5:/599DED7)41/=C0-070A)02<86>53;7/+-)'396:>CC77998:<G;2D0)&(*1:=<8/422B?,()4@06//+--,-0=/(('8'$'-2-),.16:=<B0ADC;74*102/7*,(,)(+2-,>=8;1>1&+&'63@2@>/25:2,+&1)'/&'%<;@<86=:>99,&50('-.)//(%$
@SRR7477814.1.4343 92a1b14-f10f-48ba-b169-fa390630293a_Basecall_1D length=2643
>'.*))(*15?679FB18;B931-%,/-26/-,-,;20582B=8),/0)(%,122GC69(+22.,,%.*/8=1(,/201:;:7BIC>6=@8=:<A.58=671+4*,-./0;,/6+90,/.3(+&')')01,/25'55+2,25A;<<>@C456C+52D68:DFDEH53310)%,),,,2)6<477:AH2+(,;891..(31(+)%&%,,))048=?>5<9A9ED6<5;D:),.2.47989@779@<@8:FB1CH5*CHD53.220.G3)*'(2=:;;8,1'-67/+%%',+.&'22;547.-&')**//,'(**&*?F<?/AB2-2&2728=7-,/,,47C.)3+,)3...:;'@//-5'+0),7;=47:32/=.;,106;H@93<++*0,166:A;01+/5&%%,.98:;2.0362=@4FF702327;?CACBAC6&.+210-%&$*&-+)+*0-/)/-*+/65<2D.;=<6DJ210<+2323?B52801);58/4,,*+-,&-(5:1;A9:''%564/')9,:73620+(*,.0;75)*,7E67+(&&&)-/14GE?D973/,+.788C3&('8E769.-+)/442B79FB?BA==1'.,437442202FGFGCFICFI@6>,6194/22?@>&/19394/;50.;/BG9-04D6;,++-*,10463;3C<-04437=13'.266>D@?32+)(/E6*15?=8974?52.<CD8;=78<;1F33DEAAF6D564,)//,:;<?*-'-+9148=E977;46DA;3,-*-',44+9+&**<(%'&.*3.123--111EB=>468B:7,@+2-/0339EEGAABD@;*6:,)*();.269*-812&%(%'-)416<//44258,/04-0/*3//,*#+./.2.0.*-*0*.+7:C;20?AB=505001:,20:/779DB:9540/$'(('*)125A>=+4*09-//A029<6240;D=;8<:6333-60.*2&**&.,:BE)842935?542-1:4026<D><930--(2:=8<C-*2*+.77&-.)+7<=ID3>05:.19>0)**+7245/05423183+/*/54:;==6B,C@29234KG833265:=?F2-.'%?48F1(*+:50897<9;@AF5?:55>?@DE=H>1,1;4430D--0DDGFEA::>4.+%',+-726-.,(.46A9:52/-*/20:677/--9169-2:7.*+.0/>,-243-56310353561),,,/.&68;590.1@D;2+0.?44544BAAD><E<<B4,:./.0)%&((22?..:7>4I0.,8<1%)-*/1/43=HI0:+0')0A-.D>40/7.%&*(/1-73-+()+.'-)/3,3=:5EA2.236:3355-,%+-262<24056;**8,-332./1-21??9G9:ECCD>..),(0.//13:40))'3-02)*...('(./2+54116B:>6,-/480:4;=9-,)((-..'$),%%((%*+B//15321279;1=<=5?BF39B5249;<7455<5;?>DFDF752*+*(+;1.'+*)**(./**&,5123-*.7/2/,,2999235597.++1.3<8@>8@,*-:174<*&*4?A/15F?BI6349;F8;<>15**6&.,3.688C?6-*)4,>,+6+*(--,710:>8:8;9:<51',,((*-.'**(+.5C88/('/2D:.0.=224JA8@A=@AB5.,&*+.4533(1(*%&$()/0*//(1(.-0-41,6>;204*/56900.22.D@8,(+(+105545762<-93'-0/18/.(-93241-,?533115=2,356/4,)('',+-08<:525,('(&.0@23531-/.+--;;16690BF/4=857/*,.(+D<=<GE76=D.;?>/,;:DGCD6AF6;:8*+,-*.74E<;?:9/5'-&%(*0117)4+((%(00/&**///A@<6>6>(*)4A;01AE>@)*.,-2(.)%)/2:,4)*+*,136ED4>B?:HC85349-05<<CC=5<3**,(<16464461@5==@IFGG5:201;79288-*/55/++(*+'(':B6>31037DIFDC42232,2(/-01/;::::AA=BAGDEDE8+4-%%$*.....204.1%##-18?1/:-*/26>CABBB?>=6*74B<02<9::E;84893*+18,'$''4/-&,)'&'&%'(*&(*26CA/,29:4;*-5=.B.7EA9940++1:8:?<>=93-/1+6).:9FB+B5)*>C8:2'(/.<68.CDHA<<9EBBH?:00-.0322:3/034<7;B1/0,+1,-.-+'''0;//()+('(-+)+452@G>578DG>:?H<4*+:<;3/3=2DECHAA;4=D>323257/,&%%(.,*239/@40:439;8;3<810=:1,41:09)1'--8:6C061GF,FF21D;8@)<9F@1;H>:@C@IB1>0-:9>GC4341/'(0(258674)('$*,11AD=6G50*(-*-)/424-5.,&*(,-,/+-*.+/24566:9:6CD42F702@@>A@<<:7+10/0,1335334%+)7274<352<4,/32:0<001<EF@:52(-''1//&+.0504?;,>DD7:7237=4>*'0.2)60,*+,+66CED2F2-,99;3<-&255<:>354;;:49(1*/-))()%2)?>9B6G661B?711),(.34%+##%
@SRR7477814.1.37398 98d1f29-5559-400f-a541-68a11cf29acb_Basecall_1D length=17863
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
@SRR7477814.1.4804 199b534-9e57-4fe2-972d-02f6050b5eaa_Basecall_1D length=7265
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
@SRR7477814.1.31449 df52800-cc41-45d6-934b-93a568722d21_Basecall_1D length=2333
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
@SRR7477814.1.15668 38b6653-402d-46fa-8e35-2752ccb15803_Basecall_1D length=3211
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
@SRR7477814.1.29246 cc5453a-b179-4575-af79-d9957deb36a9_Basecall_1D length=5221
>%1),/528<F.-<0-'*+73((%-,5E556;&292@2/1,-,1(++J><G77.1GF.@,00:F>97@<<1A7%,*-/,+=.425BE=847FCG>A6,);5=@<<3(&&(%,%/333*/0@>1(3/'4-*-.678883(,++45B:,,9-5:=G?=1;/EE>B=<=>ED>AFA./<31<,+/0,,4EA5'..//44>.-)0):73489>EG7657%?'()=?A@.?AE@:;;5B4/-=:@>;=;6.6@2<,.4$0,(1,/')102=<;637926@;8=:<@GIE>G8--04F96/9&,5B/./.1103-+/)+'%*743?:898:3D9/(+.28-.;F>5,9*+)2246FFFC9>E408ABA8;.//>.=E?@A;7657:;;3D1-/*.8.-7@75?473/-24-.-5C;063)/6062)),*3189;=/<H10?BDC?>31*((&%)%(*9*98)+4396A>62336=<>98:75<11502=<G0+/=@A<2<=5.177@EE9F34355=C:::.7&)')/2<1B1:90.,<:2><BD=@2219<D-7,(-025:::C9:<<7=C<=DBCCF>==885;+-,+-9;'((+*0864*063.1?FF9GH65'@?B9=8C@@BDJ8424/@:<IHGCA?2';16=E;=-,-1/.;:05/,1)-+25C.+-./87:3A)*688;==6:330&035=;331:-,.?/,(-(;.29H8G>56=ADEC//+,*77>74'@6,-:.258C>=>>=<?G562547;8>6315A17D/...;2/@9737;?<:7C0039>>:@>?>A7G+5,=4.=<020003=DED?/9.;7=@-.534<<=EDGHE8G;10GA2/<<>>@444+.70,'&.;769B0=9.=4;7)(3-A+2*90E@BHFFEGH233.)+3+D377HI00/9-/*-<=16>>/0/0)'.,*(+/))47;96C8C763,1+/5.:,)('(,-//)01962:&%)-'05345-09/50.?@*+.:>9622//.?@@,7-B@@;;?311//14?GF65*--43668:9420.%2;++1&)/4657G?8.2///9GAC?>A98845.(%'(/2EBC+,@;2022.+-1.,/00;=@48+,*0//20&4:;==AA6/*+-,1467)+,52@F@+(%-(*8,,*).6;,('C'%-3<<882-06/03B9*4=;(-228E;:59899;@?>4(C<?033416*.(',-+21+,73;.7443>JIF?>A>=E00/-5(*+(-7806+4964,7)&&%%(&**.00/12<3467@@89/1/:31;A68+&1%&(/.F9A7A956.,)($/+55234158759?94/?ADEHGH11+)).)-)&(.+09*-,,+/&.<4-8--4@4459,9233AAC=:(3/71/>C399H88897'+,89;=778G7=;1''4??C?8/&+5658H633;;<94@=BE0C95*316,,-0/1;76<HH300+/,-,>2@844.)$+'),)((6E0==@<83626D534<=<=?B8-.'%%&(-(-6-75<HIFFFA,.-0.4<32456F3486802./2;@>32240--/:J>9ABE9(.6<CCDB---0<*.;-**++57<>;6.27(7366GG72=E>:98749BD::;:9=E;70+/9:>=467;(%)0.-)8>20+50=4577>?07:7A7<D1-+&--/34868EC.16=9@F=56:@C?7>89F?215-,095D9<=<.-,)+'-0+022>54,/276%),29-25:822/,*.,*2-)33:93*$&+'0H/F/0'0*../:FHG86352<7??89:FA,4@BG47A:02>7174>BG.90.4GF6..$'2>>3-:6756&)@*,/4=@<6D4@34+1<,8(.-?FF,B>3/-)*)(/-03E=>>506,3+:A01-2'),*-A0;>;5BB?//089:??@:-*,./.0*-*C9A(7BD.*)'.5,3103:B;<?>8?D@1E211*+0696ABE66?A>,;0<CD0,+7-.')%(&.)''%)'+=103;EEC>@JI??9:0(0&=?83$+'>==EBDA*(0/>ED09@/624>GIBAAD<BIC2:1;E?:-./-<9<@H9<IF@13.1@??)/=,3?<8-BG:39@+($'3(,,-,0DB,5/>1'%),.16;/-'*)&(*)*2401964+2%%#%199/51012100(''(,+5:EG>?F?CC9>@4EJC098;:;C4>H7,683AFE3A158;;:35A:*,7B<:=5-2'*:;-),055966,215341/.27959=31:E9DC?<@8HD99FD?EG6HI-5+/++,B+-B-+88<+-<621/104404:/8<5>8<<<6BI;2278-08GF8145200,&*&+*625,)/+4-7=,)650+:GD0788A;(0.1.250,(&)2A9?((2+-)0/<6>/))'*&&%'&(*-7876:,-/057'..C@E<6477*%(2)+B222<>.1.,1)07?DC<9;<>EJE24?533>9:507445<7+89=6;;GC9B6781DF?332=1;7@2-12E1+7B999FCFAAA>:8:5;?A=CC4<1-;>61)($,-+A>=8B=7-,3+2:73<HE2>:D<467:=/60--,,8,,--*1+,*,))(*&/*-0@9;23:.53D<.3-109)+-0+>.-'(*/023B1579422(&-4+)3=<6:D?8;55--/(+07.1/6=69>;02/342B6792>00A,DJJ23F35A@:>?;=JD@F>?FA>=430/.2<01..//@.AE;.)07=>/G,)D;EA4)1=5,-@05216253.&)+6E6C88.:75DC,5<,10665072ACEB28/,+/.3/,129325/../.GE61.,+--/,-)*+,>DBE<?;5:A;=<HD:G<G9F4&55@BCDAEB676:;EDEIFAEEAAF96B;99;3/23DA<:E>1-(/.19/.6:7AA/5154=4E68965/13C<??9?G?BIF;16.-:7/*'(*2'-%&*2-2<,1647/&0.FH2BFIGH:.511924*7.;G>.,:@@DBH>>?=C43).,40-/(*0)E:13BBEIIAD:-A+.*'/1066313992;,.30+35*-*;,,&-.-?:9?--,+*+/3C;+')->84A?03<6&10237;22.:503B@7<<./8A77955>6243'+2D=61;A/874787;ICD<;<@@@+B2454=C<<=<*)'))258AAH>C@>0:F9;:DAID?GEBBEDE?39-*/21>?<?@<<604094B:3B/3..-40<5C=;--6/+.1D?>?B3876..1),*<<8=4:,0.*/--+3?49<?1F312E5664/-*'-%.+'0/13583:):64:0+..06*,02=24,+3655/55'=<?EJA91/2.3,:BEHA+>;3'(.30&/0323*,(**'+,1&2526.87E.-*C-5CCHCA>1<:C14)0.@=EAJ@.,**,25A31&,-1<B@ECBABCD7;32*(9:;;;<IIE567=08+5/++?>ECBA;;@6;E>?854&3/(%)10-6569>778D@8506(*/CAGHABHI5;/++.;:@3245576G444A?=?DE370-/4243AA5G555>A>8/).26986?=DBA>A;AA?>43;4A9@?CCCAC/030,1:0,+*(&9//1677;:9;9=?6.(/,+,&/524>A@DDG70%(,76;:,,--4:1--2-24CD>?A=:==@BC@>?:<044482*(*0:78A732(10674675D>31,((+06>=D>@;C-,+&'.)-99)./816%))(/07:>2:/*(1:D1@BF6.,&0-7118.)3054200343..*(-=257>E=2::7/1=339?0340*.,5CBAHI<=>B=C<;;GB2/&,1:3@5B/:947@612210.3??BAB9@C@?CED<22330/104;BC1=1007;9<714.1.(*+-13:@-))5:=3;CE2@9)*/3;645>C281+.''(;1443@--%-+-/:9C77570+.-.80-.&&$)&%%',7-.7B>7=52/.%%'+,F:?54:324;,*3DECDI73.&(''$&#%()&)*34795+996:>2E05(03>;+(((***.426DFG3;BCA,4?.&/;/7/,0<::3>38122,)+*,,)+)/'&'(+,*-,%+,7;/1-253/'(1,.1;62</630///0-//79>EGGEEEF5;89,@@;0-0&&&,*/-**)6>&+(%'0745=BB5:..)1.0)2)=7?;@AEE@A--$''.*4'/'(,314,13+))%&'-''&&(/6./2)8/+9*,.,2;F667-B+)'-',+%-(648*:AE?66:52=+*///8*,2;8949/.46>7F=7910/*,7779<?=3)(6)4*/@488C64&'0=477@D1110>:<=84/,-)0.+/00?2A+(35+,=,-#&+.8>1(10).A@<;<F>;.A@@AAGG@A?6=4;B</,/*../3.0'*5.+./*.,71-.5/(=B3;7??31*+(.+5,?D4,1.'+2<;:..03()*++*,6/3168324.-,16++)6;FE;AFBEF@AEB+?+ABB<9(&)(+66:;?9A688BA??A8<<E=DA8?603?<@E71+087D@=2EFGC?3.@9>H6/&,+-2(02;CBB@9>)5*((/0020607=,)13++),(3-*&/66AH;604,5/54C6*(+./.224.6+.*3-())/@D?<4F6;'*(%)-(-517<030+('%(,0365,34.12778957..0(./5)4/=1AE@-/*+@+?>A7,1=:>BGCA@,8++,6<4979.1%''('6=;C@5*)%##$'-11::5:F;./%+'/2+/%*0,*+./7B<4..&(.'&)&.5/-9>/00/.-(*+6?:@7A0FA<<+,2:/7,43:345@64.45=3041018FF?8;;?:C?5;ABA:@846==-@@@A9>6<;>@?-24;<6@?=69:055;<;*6('-+''+<-*251@8<:8055==+,&()-0/),))17<=/A.+/-532.05=+51*0$,)/0+**+2/.++,$(--3/(./242*2,+.0/,B<?===<6?+:&<?;?194(%%('**-1::9D;B>I=;6*'&)&25*+--)++)0()*('+(,3=:64/?6*+4-30343B565>4-35=C9457;-+)%$*&/)6+/3C7B/62+1->GHC@BC>:;'&*.+/148:DEC;@B9>>?><<=77&5,-0D<AA;430235535:;975?7=5?A(88/*4106<;./5<3-3-1,../.-/*-.:.-/-.554420#)
@SRR7477814.1.23407 52866dd-460c-4555-8ddf-6a76805fb7f9_Basecall_1D length=3821
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
@SRR7477814.1.23403 0a6f5a8-f76e-49e9-b5a1-15abdf8f1db5_Basecall_1D length=3845
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
@SRR7477814.1.26224 f3cfdd9-b444-4c96-ac54-b3997fbf2738_Basecall_1D length=10452
>'($%'*28;54;?88=>88<A<BD<>BB8876471310:61-49A::F)<C8?HG1E=?8CIE256(>*,<55136DD471442DA@/,('+*.ED-.+65-.:1,/1676797/,12>C1564DD9+222521317-*'1+*0'&)964015C11+)+2344CDA@344<73>:12D9D@A;>?@:)512B=88F,:;?7571///.:24<2-60./015=>AB53574+%(%)())*8?HG;9D72/.01<=9E<0<E4)1,+.,'+/36->+1-0(%&*70>=C<3@3G74+*'(((('+24+*:-+2</2@C?@@CCGDDAB@38.,.,,5..>63*1617229783;550(2443299DBC1=%%%(5F<A>ACI>9:+8034=:5<77./2::?4*,,40>FAD4@-=9:1<..=30-./47CG=?@7.23?DC=:2464-565:;79776;331A2/+06<-//628259;GD<>7=?<)(*((/1@5=;:3+(7@.9/..5440=?25&,(-/2-+)+8687<54;->/2);A222/9D>CG;?@CCF64.013A((*557)27*035A@@9869I?DGD;8-''&&/3+1/08>1@@C4+/4'/715*;6579C?@?45941>=E;798><994/95+$(,/(7-%0=46:33+&,%&&--0+5,.*(.)/78AEB9:2.(+-.%(+99-*74EF4,(--//<111AACB58AGGG:-A4>((1648*,-AAADBE@>898;;4*)*/(,'%'0-/75.*'')527:56C7<@?FE20//.7>4024;2;?DD@AFEB>CE<?3?/E.<?7DIH0/E658BDFECFFB?5601-,&(*,DCGA?@C@*0208*(*-+%&'*//1713*+--.25-?,'%'+,.:?<0*(/21(6*)*,*',)'(%'+')2/,-/.=2D212050$/(42+0'$)2)05/*-,21=B:182&%(*230D94+.?8H/**2,--&))789'%''.7-4).0-)*,-6GF?0DH+;/3*-(7?H<51')*,-,.++.((++,1,,3B324J0@JF2487:@49B39BD<C?;8<8'8./7,,(>3).8792BE6-/455B322349<14,..77J:;274CIHG:@B/H4)*-25>=E:=?8==@B@@A=:;:(+,3B=22,-/'2-1,,)'),)---11<745'3587110:@E6;@<==:?@HC69:156/32.-()**/(3--&*&*/,,,91637DID7:<;9>EE@@9470,+130,/02;G1AD>C5,'&),-2D8:<=;():95<>.@>84568701+-+)(*,-,***(,)/,+*14>;7889/3.,*/24,/..,+&')-*%$$)-=3032=<D@.-'),51/./02>78+5439/9//.0/-/33'5252.(..2260,18<6675../.*,&,497<7AA9,,-/3888D4.,.7+*&*,+.2630:61/++).22>C>=+=<@BB55<;106=EBHGBEDBEFCH>=5599<I<*,02546====EB<<4752<=DEF?7:8=1B4-69/.==GC/'.*&./<?DGC>A@BDFIBA9;==C11'&&07;:39/>543;8;FEC;H:81;<5.17<BB789:BBE2C.--;=;?>9?/'/+.1>@)''(*1/22/0AG@7:3=7<=99*-**+32,0658@=@B?<;..1242-0-1*/*,6.902=>11=>G6+143>2245)-*2=0-035AF4<.A965:??F.)*,0#(**4./C44;67:C:?+/.G,3165@52*(+-./.+0-/02,)%&)++/&,&+*2.1?4429/+10...,3&/,6''()0629;76:359;9;C@16685*--093:((*'(,;5&2.,**>?A?C:7;?-(4(//-/?=A?AB5EC0.6023EG59A843/.%%..&/&#$+&--100**,+0=;3DB+2,')/1446*-(**30A,(7BCFCF@.=630,..0''*+,;:9.3*(,50+1.))(,62@E?><:;/HB46,&/1.0<E60)7*+,2/0')-13<0553,<(.'.10.*&(/05=3>4).()-.+/.09G>@:23;05A95;/)8308D7;11/;3+.,,%$(,)(7:+AF9?)(+..2HF/.)+<BE)''$$*-5A8=9II;0//0.AB0-30;9/2<::>857>0>.2@44?4&'(+4430:2'''+-.=3.*'''-B665F./0/)((+((%+'350630.40>89:G@>@;888CE=24530-2G34551-1+&+39C6@C1=5A6G(5>.**''36.11331/6?::IH882=EHH:537/8391((*+-CBAA7F1..-*.-,6B?;,83)907*'397<4,%%'(**,08;0@/)%.(*CHFD;@:4AD8'1@,-<<8:;EGE.D<=?8*3+3=7>F?,(/043>:;CB=7)2(14',0/1?AG4H8EC25;@6C85-$.017:=;7@B>=D7?2A9=4+-054,1-6B746;D6=8:3;62159853*.0/)2:;>:B9:0,.,255556120=10..*+,044+,73,B,,(.)232/016@,0,*,*.2H2032(0>B79-/,,0-,,03C<.*,+,/-)13;210186544<4-/(%''-=53?B7=5(1;49<<>:??E5/+))())++/594+)$)(68.,1A5.../.,+')+3478854/,/+-234GC>@E9D7338889;?8>)1,'+.398=5;*(&'*+4A4312,339=6A98>'556-2//(1=?=;2+,&0/256*5?102317-2*/.05(<>F6=;.71)457<CI@13174./.25,+'(*0839F?//0/1>>*892//2'2%&#(.)*-?4;235164A)AA32/-))')(---,47236DGAAB,-()+5&1()*53=CD>?A79<>:9,**++32,-(5-.FA9;5D0FH?.--0/6<15@A>-983:1?4').24AG@4:7//%1(,+*.-,)%&'+'/1AD=://(6,&,'')%'&(*')%*-/0;1E2DH/6/,850-1>IG16*&/,-:?==2=334??<<2+11.6>67C8572@>+..-04+*788768585018A5,,-:2;:7?6./?@DBC3/+1,99-AFC*5:;:@F454&,)'%+)-4=5?4789<E175:6-(B.()+1*.+12.5=89:EB;E9998?:88?=8<<>:62:://37:-CEHH?==?2968181/')('')037.>9*+(,(1&&((&,-/-,,*83/890.35.6,-)-,0+,+)9/)'-,F;>;HE3E2'/:;;BC379;B923560*-.:@F..23<.(,046454-()7<40)*+)*416A:@2:?:/>B3.314/;-?GA20,3==DE@85=B4:4<45:1'**,:9.+/+07?68/1*+))*=B>?C>3?4A380255954)($)0-,2;3=>858>22<:8171,3.E06H2-0?CCHH@@8++*('--0*///+3*/-25987871+/:?99,16??54'0-22,,226655A>CA<,,:32,;0+.A+D69/)1E2<+(&%-0-82<.%*=C@>@97>6@6.(-'6244?191*(/,04,*)*-7('+46:446393/:)=8;A;3.33-&(+.>@76996>3:232::989H2@*/0'3*2:52062869<=1,,-18<E>%%(&,*,./A0//*F=>E:5C5847;;19863.))*0?AA/:/-6*.*7-('<913.%&'%,+*$++:+,+3-48*&-/=>B43://.0*9;)-?/?2@@25?8G>64C('(-'+,3<*2'**3(-/03.-30B?:3:@E2A>;+.+(+19B79920-,,,*50.%(+''*.BEA19=-$$341166.8@;=ACGE=FFAEF3H1=12,4+2)41926()++/<7634346=849<@@,BECCC+53/-*2,3379C<CB=>=>?C533;=3781/.-,9A=@A2'(+-2--3853A>:8,;@:%'46++***-)+*.//4228+1+,4<1(-)4,2,1/640*(#&)8,%/+$,+=29-1.3/=+'+.6;=@/-+-(;<0456-',.12/0,*(.7BB1,+3036314:I=/;CA>3,/7'70955-/(+422,(%'+)==GC55*/(%*')//204*1'/<=<@741/018:;?A@71.)(),188A9GDE>EC@EFE:3.,-.(/&9116618&,%)(*))+,->4.++,)/./1:@@7'1/'.-+;7226*-6=34289<3;7;;G;<-;.3)'+(&/4/5B:6;HA88./87,>6CC,.@@5337-56/:;9=<?FBCGJ2I2ABD@B@BG@=<?BGHAE3=,-*0-347>7;;>?BCA=05'&*+1.,2.091..1:?=89416;6557A5>>C;@EE/)/2@9;=D?DCBBG703/<57<:A9>>59958/8(.%'''%16:62B<G85>:=C@CHACBEE=H982-.17@GEHEGEEC;DD5(56;<43.2+-*0,%-.63-.,&3*854406((&-/52784*,-08%,)8F@/96;33542158/E2,)21444-*++-'0277;0.*((6(+,?>-EF-+;6AEE8)((',)0?<675433/22345-1())(0/,)2878766598633F-/*+'))-57.?@=4123106-3*)(03263G:+1-1-+/12;D25C;9>3173.:6=H3258(0-=224G5CFGDF;13@?4C:<989>E;)&2/:1)*(-(1477:312/6<:8;=3A/187433.55.*800.-8:2987:BC:=68601483=CFCEG43343><>@CDD6BFIA==;8E>68?>?9;5+%.-;A23;5/<5034984A:;3=<??2=''10,49:9B9.2/-(3043,-.012=11=/4524'.))/(&(80*4);:38879DD5(('&)8467HD==-,*5/'*-GEGI1FD/0IHFCEE;71=1814.:2/3218A1A2@10.33+-.-5?ACBFFDCDE+;*G?0+0.>4;*<6221E4=480/0../;*,/:6((+-+*,1.6'.285@?:?>6509352507A?@:<@>A9;<?:50;8:AEGGEB:,++,+:9,&,/3523&,-2.)*)2;02*618A?:=;;:63*233>>DGB23358;@=?8ABC?D<:>@BEDE79;;;EA?>=)))119767<3233;6;6:C-2*00)2B34:5E=>>D/6///E1-0.27BABIFHFAD@;?A491%+.0/*(&&*13,0-%+&.5/.615C>411362-+01BEG)),-.;0.6?0873+(%+7264?<8234>535+/'%&*2234774*/.),3153->?<:=2-28CGEGFG.,+32,3.,.+A-+35)34>?0//-&88<@@@75678@=5900+2.)10//)-$*+7@76.5.8.</-3+-++'*-87'9@:EJF)<F2+97:=6G676CCBF<<0G7@FF9F:8?:>61364=(40558-06A5EF:325043131889<FDAA???CJB;>617<73>3021/%-*(0077*0%%,+-/0-,&,((.2-00995@>@F33'/7*6-,++)-A85>;1=@1,7=G>?37--.365,320)()&C,413'+/6@EF83**908,145C549@<<4C-/168;7IF976D537C::58A=>3(3G=12GG9995<FAA@EDB=?;/621/010=:,8'6,)+.1220.7=>@BE9:9560,06.*,*3-BGH855>4.+((',/0-A@0*B>F6-2;:4;2+%*++==:19436?455?F<31),.:)@582/01340;42355++,-)/04.))+97*1%&(*>D?@3/;00;50.-045434AFFB4:GBB;<<2=54EFB6?1@43BE<?50+*+*-2FF6>8B88FG35./427.+)-'03B5@BB5%&++97+)-62/-1-50+@2><797:=CABAG?>=9:0/1314>352,+053/4368?A/,*,.?-:?865B@@G?G=5060/.+5::?:;<292217<>7,)-/.0.200&-13=9;>BE@?=>8379441B55C@(4>.+()13B?BHBG@C7.706.605:A1975<;6)&1=24,/67?BD&('')19DA@.7+'%%%)8<?;5)-((((1-..(2168=@A94;9(6?-1/18.+023FA@CDAB212,7@CDA-7G9++,5B>A=@@;02*)(02&09CEB.=:>:<:2=DA;<=;:@?/-*+-EEGCCCA,0=7B;;1.'(':5</8==3>6GF?HF7.-..0?@@610(&,.-4=/129A=;;35<A<F:674,-*43;=)9*'&5;-*'=7;9;46B?@0+F?D%4-36*3956FA@BB0-20D:4/-0-135?9>7<95/..,+.0>>6E527342-=-6:5058E60EFE2AG=@<AFGE894<0DHG<2?7882BHHD8::415A)ACF<,82779813-002(+14.*90;JHD-:79335535.>/048>*@487?F<40*(&)*076'2363CF1/123?=EBEB;;:-+7.&-3;*-00*&*;1/-1/BD60//*-.B>F1--')+/720'0-45-9=>B@5BGD8:9492+-5,6'0+*0008(:,;/@?0670@G:6048.004;3FIG>466GA31=7*00.8AI57@922+.5DE223E9ADA2656:@,-1543=GB:FJI:366?<47C4.8>@146C6.:=.+,)-+++./49F:0519636<HE12@?19;49:97:87664;29?7=,9/C30/&%-000,&03052=111=E221(+(+147H:746757C99E@31/14GC=998-.//+-+.-4:E?3B(+-0(42+'-/%'*'4&%)369=:=>A6CGFG1213@87;;;84F),<875<B3CG?6H>32.8.,81/6FE/1BD==**+-:4302//17A6@A@A;,7F2/,0,()3=CG:78-++2,//066484()'+78&*0:/3BC157755./).&(-@7DE=CHA@:6511.-22*)4,/CGC44137---&-,10*.3:9??>71-+2/3/2A7345:.23=56-4,,29>=<<<903=,)39<8:?E?713,-/7/5886)1+5=1@322C?<9:)'(</A>5>:65@@8.+**''258:>724357:A85;;+5.8EE77025*/1;4HH89A;<69<971F>4+4@934116(3A7/0$*(105366A7>;/%)$(&(14)*-,(40;52'*,:1/03==>3:71-3&**))75)*1<4.7A>67EB:2H../4)A1-('286:2C0,-..,0.1382)6581F1?.;B@JH<F*=/B9;501'&''(-*,/B0/1A06-*/?=A459?<;;<>=CEEC81/>C)=::=5)0+0249982,5*-,*346@C,8:>21CA2282??<)13@?<,1BB>467322&%,$%$7)35C4>/-*07=:2,/415,*+25FDEDDAB6*-./:;@BF@>A&<'569E94@>FFE8+6:@A>82+-(()301)..7=6<?;0-+2**72/00F6440.'*+1*,*(&+)DIACEA5?)(0$-/2<4192+?;69;6>.-),&3.5@B/*4733)2,,-532->,9CG6+:0C?3:),*/4(<+(6')500,-'710%&(&&,60'+<4()'&...*0//0:;(>8:816/+&)0,/16268FE6+*+*)16+/29&(',36A2.7/.,<AE70;8035:454*.46B443:17)(*++,,-D?3253.:2.**-740.14/1446BD<05<=11<F=46:D7=024;E9?=20433:C7B?A=BG2=A;2D87CAC?=>2ECCA@@BE<>>1442343//8CDHAFAAC@5@8D=FCAAGF@=;=A?46::210006C779CCBB>,8(+*,(0-C56548*-(.6555945878G=HDB6-7-(-EADFB*9:/0,,/*&*(/-2.*,-550,-)(/91.191:?F?()/+.6505>9AAHE@?.,%)%0%*24%,)3*3,.+-.+,*/%("/,-,.*-575AA4@E1HF-0()1+9.AJF:=@BEGGEC:*&7:7,489@>BAD@=>?565>895?FGE?@D8@-/+A-+-DEFDCE8>@65:<22+)($%+*//8;<?5(*3/-,)06(*&*),6-0632,./,-(*/44*6'++1@8897:-@//0>?;AFB@0:-1738;?EB8949'2(+-07444410)%53.*1001?=8::/>99;FBA@C2031.26917D118B<733010/124)<GF(,%+.(*>@4;IDBDD?,-(+/.15/77832,(1:7AF8?DH?-1049B>50.0+--55548774;4/028744842353)+..74C2764=I<89*&/00)+$%(*',/+65:?D2/200694</&53-57+,.2+%-+0-/*0,2),'-:5;;>=-)'(&),8;<B@E4>.04>47/2-*&*(,16:H=7EA:;G=@?::?G.//CC6D=F9@=>A@/?4,,444:;51,)-77<;<F@C@?:&'47&&$+../1.-'&*+$)3196,+($$*&.,*.((),.8/,,=FEH/63?/0*.1;2D344F=;<5@4C1G0/4CB:4--/41BA1I./-476869B@485:59;3/,&.**7873;8.0--*)*1253674E8579;9:=HEBEI<=8BGDDA?442,:23189=;7=215<171'%+///6=199/35980)13AG?,&/35-9156B@4:,4)0&''9,7)),*7576.0696210&-.8.8*.177>:7>/36D837(*'',+.47=EBCFG8,,-.6900)*(',9225680;03<,12++;31'/;8<6=*(8<**:56HGFDFG99550457=?G>ED;98,32&'/*2455<:=C:9=?9*.0.3,:/4::EH?592.,0.05:6.:)5<6724(-&4)34G57>:34459BC<=311000/25892-1/(+--23>0.)(%5,+85*,9+-1-.7A7951?>4455247=;8,2*,+'(((&4:68GC;A:7.027.01/,,/+21?646999A>B?45*).,360*&)%"$--(+&(&'.4.2-06)-:41+,(-$'%)(+--)/9<>>A3???A>:>2,&0+-+(+,&')((&(&,)01DF57A;<A7576)8,=GF?BA=6//3-31/271,6>964A:>>>B??;I46=;457,)*13@463CGD=4:97=@D/-+++/074369/343657851/*-*-+06,)/4).''%'.:450289AE41239>>B:GA04/0802.12*/0:?+''1<@3;+B>6B.08D9A97A<G(/>3.>2157312?GH>5:DHFAB8<7:77897:?<C238<E.CDE-/3*()(19@B+;;742=-?/-%,&++.53-,.),1?4<BD?BFG88),+.'&*&*,'$-3/BD.0*(,*##&'&,,01-*((,'/-66/+.01H@>:305,4E--842..236?DGBF@E369:BAB>:<774GG14<F93.24>@<4=315DE0/0?B@B@,/*()('+>-0<<5:9::5B::;D1I-,3,./3((,=>@A0;67&=1..:4@0**.,3)4,+2,+CAAA03/,-0,.+-+*+*,466E?8?440581&&%-+9?0:<D1-/0+7)4-*+.).2/G0815>15CA=6:339'/-249:>8E+7-032:==D36673:?I?:HD<24-&)*)*%+(+)%&&%)0(3')2&&(,555/61)'>=-.+,)%&))/,*,181.-0748?9468AJ7,.,-)((3;:>7.2/,/-9<=:001<J:76651@5+-(.*/+(,-8<;C66C89568650'),23;CFCCB50/)+89;:9=@ABC=G-AA=B:D;H0A/%'#,-?0/*&(*%'((85()124='(,.183108<53:@:96>64/-5-(+'/29C=,1(-&**8<+23,8)/,-/<@DCBDEGE@84>5934(')-:+;/.0125.=3<6437@:AAB<@F8H.9:-)&.2;8>AE432+*++-<9C647-..-../+*%**16)4:C;8,0+.*)0.<DB0B///G3*(&%'(7((+78+&'&%(;8B@-==8.)*.0-4@GCE8H454E49<A6F@7730/7;02148<B:/7590B?@1>2?;B69=<=38.>..12/6E21/.-@AB=?>+++2'#%1.6=GFDA>54&%'%(-9;98124??=??@-0-/0?5-/353+967/727497@A62,6364:695?ED@@@@?0932*).3-*+BAG;599-9F@99BB@=C@F?>AD@FIC@?>/*&)+87?;<C2>1:,+-(&()*27=A6G)'--*91/.(1139::8125=44://8/0221E0F//&-)+/B:+;>+&6',9-.6D>22%'.)).135*((+'*,25()(-.>,++4D>84:131350/658;>>EIE60--++*/)),?ADJEH)DBDGCD/)-;.):-6(+/&&(0)8497?1:<==7-,/<?<=?=5C6.2.'4;<:7>@@D6?44.<.0'000415(+(::75(?=;91,+0+46?>>;10&*/0(('-745;<>>?5<476;8:<<9()4)+&''
@SRR7477814.1.41075 14a02f9-7e4e-49e6-b7ec-12a225d3ca7a_Basecall_1D length=3354
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
@SRR7477814.1.33612 45c9b5c-e0e8-4717-a906-384c976f6ec5_Basecall_1D length=7778
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
@SRR7477814.1.25972 b560907-0329-419c-a67f-a2deaca7879d_Basecall_1D length=31829
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
@SRR7477814.1.24879 64da6a5-9990-4449-b41e-c7416a0417b5_Basecall_1D length=12710
>(),-(..'0:5354B,32&<1%-892;6,06.<1,/>6.*&1:3244BE-=EE+513%08.,,55--.;//(&/5820412?.13>)),5-++@-F=.01.-+:32&*+4<B@?>0-,,)*)$(=5C*+**(05777+*++(,)-6F2B.++0*+'+-4?:--%&''(71@2=11/$)0<A>--.-.335+*))((&)3.++33;)0(&*8941&+*.2*F0,,*()*,+(+1,)''*?=-111-595177-1A<4<0/12?49&0815=9E>E@,GHH26'*/4*)*&'&8*32$&('&(&++0<2540&))+*EC54')&-)4/.+0>D8142439A+1*-.,),(,,(*'26;;4:3/34-'*2).7F:E8F1.*+'(&'**>H;FF8A62A34@2//B0,-%)3B=73815.,.'$'$+B1/,+$)&01*)((''%(,51032863**$'()-4>7=J4>?)5IA5),*(&3,2@</76/,+((+7:A.40,.+BH4)0/.53700*+0<-+/365+*)*%83*643..*((+,1*,(&+%,%/.66:8383+/=4:42-26?1.//./%%1215D53304+8<=6-.*/)).,+0,,041+1/0+-)-2:63.6>;;?@@;BD=@8BB7591:+/0483.-7,*')*'-2=9;495B654<;5D96+1012<8=7985:;;?CABIFD=*40./3A:<9=A:3,+75A:E:B-)3/(,1,:?,5*)'1+'&(*'(,0B@4/1*++4A48:=D?BB674,9-.,-('87;*)+?AB:3((&%*'''+,3+*('6:+*&/*+++/.115189<89133/10+1/+,,*-,)'(/0..004@BF=4,+++'45273-(+(--0780>15BH7-446>EB84*/100.,/>.8:997++(-)*.+**(+-./2D6-22,)7DD56534<8/,.0/947-+)-0422,?9;:BB@6,*(').0*+('%&.*2*/(%%+-,1)/,%&$0+03>1'&1*,'*'1*)5F@@BEF8;785,67>?:,,)+,+2657=71831,3..*0-/7407.0'(*()&%)$-*&''+('4=4+.,,.3@095;7:536E@:6-:3;AC67?56'&+'.&%'(/-15/,.76+--@''&)(/(+/5,&)*&+*2-4B56&))3?6=--,%&)*,.-1231*91FA3@7=:D9<61()(,--+(-'--(6--8:&-/9(1:710/.(%&)/,.:*(),./7-&'0?57'27)13/<'()++DB7128,,14).30+0/')'%',.,0=:+;.,*%,'-)%+-301-,,+(/-20.2//$),-,,(+2),5)))()/7=:,.%(&&&&7*(**(-//6**/75/()/,+/2-%-2,*)/1160)81(+(**,/8537-@<4(4*5,720-,&%%'()3-)*323476:010'&000.4*:34<=7((%(*+*-*,40:046FF===23,3=.&+1./;0<82+(*+/+0-10$$-4,D/00845473-28589DB2/21/./-''&'&'$(.2.&-/7836<E;6GJGD2A33=.,+-,+0513:@1;441.0'2116;14;>,4@>@<69/1-%,,((+*,1-.+*-22*,)&)(6:16*.+85@23,++*)9.0B@?B4/,'+-(-4:4&)#$)0/(*+134083(++-00046E>BIIF;12<D1<++203/2:48E61A6GC?;=BC;9JI9.F@9C7)98>2442,,,--'';0**)&*340<-4/63))*+0/3:=A;=97451.6/,.&+((*/,*)29.=B0+96-2;/*+*-2-06)/-208?-:*)(*/1;?==2,(,),63-*'*(-'*2;/;?//80.234168-2*0*7C;../0.(3(2).,2*')+*,,.5/440/1*.+-(+0*'',+(+'5:@229@656@,1+#$'$<7.*-*),/3>6=F*')0(*,&1',,'.+'($+&(+*)318)'10200..,791''(*,31',)*42/...-+/054/-/76$,(*(/=5,/(*)%'$#%&(%#'*,+3432=755*93230/<8-(,*/)*&)+)'%80>/#'/.4,*&+8<@,+,,8;IHI/',('(),0*%%-)(*'()&,4?6325>6?F.I)))4;3345,,)(((%)+8D;9?DC9:@899DE0+(+-./422((%'+-%(5@?.+/)./=983('$%((472(*+)%-('-(%'''%9.2-/)-2:7@A91=222:93.(&''(*=9/51..)%)'5<7,2;?1.0014DGD3,+)(*')'-)9>BC+E)-&+'/)%$%'(01-7-2+/+225004*<3+0295-6-3:6,(5=)/).+,.-*9-+)&*0/-42'.%$(*+/489<7248@7886168:?G:101*,1'.6(%*,80G>A94-+),+*/0/4@@>D<>HI1GIC,6/.*('+-.*+-+34768;/,3.-+-446%)&(,1)''+%3*)&1768+5&()')(6125'1967942324=,122462:327CD3887H?+05330,+)(+')2(0*/*27B**021;143?76/2)+/02:@:?2C(2.'%9'&5D@3,(--12%(%(%(*1+68*+1-&1,-159BB0?02:,103.=C1)44.113572965;4B?37:=4932.2./3+&'&*9350;A7(=96?@+-'$&&%&%&-/89('%(+-0+30*),1,'(%'())),08:/C07.58<33+3)+(2()*-+'+)/.)0+.05@433/(&6/.*+'045),052=)'.1>77423.'+-+')'+*2,(*(&'-**,%%&/985)%&).,)'+*(#&*((%//7)25/61/',/031.4+*03,4**+'&%(*0?;::88.+)152:13&+'))-2;&21&+,))'-*(%-+&&1/(+-.($$'($('').-1<3)+/),(&%*,1))''$(,)*(,/#%&(*1(2$4-12648@:4:*658:;0(($+%%'%$'53+;/.*..//-,+(*'',4'(.-/6E==?:76:0320<<9;::GB4,7?<///.(&%$%%%('%+.+,3:33*.*&&.,&,*$&%($('#',(//%')-,0-/*6*+'&')),%+$+('()&*&(&)+%'/:8,23=.,,/&7?0*..3:0,((1/79335*.(++0'+'%&*/-)+.(-/<7/07217))(+((*,8026646+8@7,-3/-2**.0,'%&&+',&&&-/12(+*1'$&''(+&'(5679;421.,&+&%&(&*%(&'-2-,8,4-03,(1.01111:42125?>A>A;>>E=90(..15D@63-,/&*844046,06A3/4-2CA11.*.%)(+974---%$22:11+2,)//*1*((&&'01DA8:3.'),0$'&('./).))')&)+7.-(++*,622=0))*,/*.2.'+($),+,.,-2067..4.).)(&$%))(,.72-<<=&5)((*''%(,04'(*/0604766:1755351312E0/&&#%+-/1++--'.(%&#''(%38&-=*')*-63899656+*6/12-.7954%'''/+/EG:4:-)/065751+/..+120<.:-'+('%(1,.70@?1EG@@A%&&0&)&02155/30516+/)%+7.<AFA>0/(0(+89.&+/*',.1+)+&1,+((*.-4).*-36)))*(',+,)'%(((%*++),&+30,++-*'&'+-&+?16ACACBHC><D,5(+*,%4+(0.+)%&#+(*15-=F?.,/>+%=-)'',)1'(''0*1*(&&./++(*&%((',595.31+/&(%)&'/+/87<;253/2.2+112;59/*13/%/*%**+33@A:>/>B1/&(),+59-023/67?;66,..1AJEB-)'%**595.5B1'5,*+-&.'%+//444333+8//0&,0&.('39,)+2*+-.)+&&)*,'*,,.@2-2622/;9''-)1&'(((/(%%&+*::78-<63)%,2.;B0158</5**%('(*,(('%+92,2++-41BG82139(.3-.&ACHI036/3469+/+),(3)$&1./',%*('$-#'%2*()E>6126=681,312.511*&,89=;+*%%)2()((+011)$&&'&+*+*.'+&((0103&'+.&3*)+369==?D0.,',%($&).,9833D(&&0'-+*091-.**(($()%%(0,0A1+,12131II4-778/42,$%&&>44>=2<IJ>58D7;7,++0//8B++*--,.-0@;7+$')1,6BID55;44>555JJ<792)*+(252,(.((-2*1-/)13;C@2@+*&.&+)8710./2+21)0...0-4--,/-?/.BHC/./-*131A980$-+&15)&&%)$8610&5*'%&%''(,-,0'(&)'::FD88.(%..>9<CB>@?;-.)+*,-;/,.0(5>C..83'+',-->.?3'<@>:<<8:896==-EHD2'-+.16.;EHHD83695??<:14296/7?;/-??.%41<:1;5<;AB(1*./3)6A:5;73-3*&*+,++($%%+*:,,:23'5(,.66:5=?.''1,8?BF>C/2/=3139HD20/.6@;6>9/4;*''214.4?1)1,$'84/+..**++.',&-5,>9+/,)+2)1(%.2/-1/,,(-374?:77,++&&@43670.-++)/)$-*60,-::?A>=;6>3+.,/*(6/+(/-//8B?23*075-17<D=6355,(**.1)'%&$*,.)),49.;124C;528047G.0+&&-?9=(<)(,.(2++.0301/821,1-0,5/3*4>,(83.3(+%++-'&$'(&*+--8A<2/(.&-87,/<,)%&$()..*++('&)'-3/5,)+++,2%+005D@9*+'-,21-016AA48%--4=.-599@311/3+*+)*3/5-1133.:/283D434?><?77.-.+.<2665;0,)('**)+,)+*;*3+-.)&)))((/'/((*C)'6+8,$,+(..&+07=6494--7<--((-*+)(*+/G6.(,')*+&+57*,,+)*,-.+.03@H=++''$('*,4.71.=A9*-9@=023GBDEDC3DID35/9'%%&&*1HA,8.*+),*0-,*3('+)'%%%/-.2/--5+.()%(&+-*$(&/13/3414/8?C?6AGG:987-&,.A2:(&(,'.)%&))*)(*'%$25-**26492),,)1/2-2(3-26=<6+'*/9151,0&6655;:68/31/&53+&(%(,BCB4A./*1'(,,//**/.,22/>1:B9/+*215)4,3(+0:1)-(0,/05100+1640)0*)6=2-/AB5('%&*-*2,(0((.&/11.3.1,44>:;<;:*.*037C?9B/E*&*$(*'$&#%*)*++'((*0008<:<;<@;==>21..''.41.*+%3/3403<-4,**&%)?9><FA@CIBD00*'&+'%'-,2;5<BD0/,-&$(%$%')/2,0706-96/./)''*30,.2-;8<@BC=F;<8EHI;657'*(')*:<-EI.1+60<=??A;=*0)').0./@>;3??<.1..+160'(0(+*)+'2)(%0''%*&&'*&+(*1-+%((+-(/19@8')14894,1)'*)/2.4-10B;-(&21+,,127'&19.%-*-74--9/C0.-1:7.+),*1'1)('/1-<3/385=@@>6:::=38+3-1-%*'%(293321-2.+4E:7:?<;47,*%%&'@;<+.12B888EB8799A:;;:2F**/(1/3547C::/-)-.)*'%(-1)')%,'&%*(+6,'0-2**;G--=A=89-1/*,9BD0+8'%('*83,-037<:<)5'6/(3374/&'0-+*%)'('29122-19/.;<@4/4,*7:*2240161/82369;<1251005</..,+=02/.,%$&(5<125.4+)&(%*&,)+/*,-:0BD-./08ECF2.349BCED1H/3-)*'-,/'.1932,&*&/2+*04C<><B836<.79;440/038@F;75:?.(%(,-117//18/84.14*)-.+-4,/0:><<=;48)$%2(00*;IBE?761/,/-7/0+-$%&.8IA5<?B2?/)$%'(*)&8'6;0190:422>1*-*0()*-/08,-*)$(&&,446<?7,2**%4&%&$')-93-=-//1,0C6<7;A;<14+-&&'%*3+/)+'-+;-:)5,3'(/.0)/32.334/DG*,%$(***%).59/624),(*)406C4*111;=3//1+,*+/6-,)*+;342<:0/03.*.8;<<9(/.-002;9<,2',.450-*/,,.-0&%311?-/578>5333(76;7'5%)$/3<3864146+/,--.*+)15170+197<1-)(20.45497<<910-)2)'//+('3:,'*-04125@7@7/91?.),;C;46('%(%/)(%+)69,(.(--8(.',+*(+//583<2?-;;7<>F27.*&/--7796;?=2B2*267HD63249?;41,*1.+**'',-74,)')3?3(0+74FIA<BG<11&4.+*34D?DCFH8:-+$7:698-4D4-0%)0&43.08,4031-77>323),1=88320+/)(*2?:/(-(.&(()2-2=6431/)(%&%0*%+)0.0/-,/.,/73,,4*;))(-2)4/8,3)1,-%+2<6=61..<022/3>6.1,')((*/0=EEG.)')331./7237A/(,%'%#&%$'*11+1'&1/0',,(/,3-1,2.1>?/,/310,1+10D=:7;>:=?1368&+,/943.30/'3%(())//3-04*,->6,*(*'&$(*+03,,%+&/3/)248B,'*,0+0+/:-66:@@C(<?2211..00117IC6DFJDGB+@,6C(((&%%('))(-('$00'),+(&+*),-.2B@,;DCD?/</35A.0+)'-**)')(-'27-$*),,+)**)$)*)0(-&(%)*435*>+&**&*?<B(),=94+.-.,<1-3+($*-/=55/5.'(%'('))796..&%',.%.039/,),+*''%.22,151=/--=?/0001>=94<F0323187/*+.1D=2')&&'-.&'*(/1/8B=614+00,,*+-@22187FE=.2+),)(/(*0:,6&1,+-&$&(,0?.-(+--/()5=4*,+3,**/0*%5,4:=41)%)%*-,,*+18E-(/+%/*00+&..6?,9:1%&+255434?1/04;.90./2.,15&1-,2-,.(&(',017<64*.:698<48/0'(&3-/-/69.+)43917+/'%+/+**1*)3.,),/*('*'**).27E<9;:6(*&)+3.5/),*,016@;95542?E98/08/.-,-47)1&%#&&67DDB;=/%8>32<B.>@/,70*,'&'''%*)2+))(5&,,$*3-4(%),355,*1.-'*)**,-+)*/+/0:8372475(&(*&++,,1++:B++)-(',.,A./:+(*(//20(65,/=$**$)/.;84.**./48/;0/73)/'0*)+.038?=607)1<A/+'1A429@:.3A0)08*2.,8BGF/1')035.03C8:)(40@7<389>542/D80)24EJ?653,-.&334-1++*.'(3,++3:283.+%+:-,-5903816,0BC9,4-52851/.-;E3/(+00?543(53>5,*,0-38,,1/+00,,+/5447+;.992/(((2.+))((0,<:4+'+)*)*'),./71(*'$&-C49/)*(0*,.0489FH.-*04'')*/02-==F?1,+'&%'%.313=<10&*#*&*3:59@5(-055625))50;A>.453-3*-5;5+&*'%'(,-/8A8D)?+02=7=G0-3.',)(-%5621/./.6/.//:231?A@<24*.0>F9BE350*2.-45383:/)*,+.'&&%&/-),(,&)&*-20/?<0G/9=5*--,*7?566?:5??@2027;=7.)*-:GFGGD4,)'&&'0,-+)2073//&-(,-101+.<8>2111=A@61.,)),+.,/,*.35B@BB@534H269:>91)048:F7:A@6366./.,.6:B-93393&09/00%(*)+)*.53..,+,8:?@3?;79:;7344EE,+-894&$%$&1(&()*,/%)++2).,/13=75/23@8FE4*)24:B55,2&)'+(&(()9.+465=642:AA3=3''-.*51*.;.-)0**'BFFGFG@=.%,)**6/&'./067=?D8.+*.9;<?CH@,1*/**%$*0.1,)/646302+),*+:0-//9:21-24,*,<,7-?;7)/,(''&#'*324><<>C50>794>746708454+*205G9@ADH@B9?7/;1127=)**,-.+-'..+,):*D*'.353)=;@?C==B-),/40;++,2?0883433-/1/($)*(*)60FA8:=6;<C77.?.,/1097C@:<;963<EC2//<6AHE8;FD,-(*)08.-()%(:1)-,-0:BB-+;//4A;64.<,>.1/;48,-+422'$,*.06B>/)/B6.,1B7/&&141*&*/'+*5=<?5:0**'+-4AI;8.-,1DF=AD90.0*-/:9691441*4,3./0&&'%-(-71247>D323<1+*..&'(('+0/01.6641.)*1+3397/.,*())'&/*'(+**&1)&**2;20++*('.*+)%.,-966219AH?7;AA.-302.=A?>;122.//:9:7;943,/60+25211:26<04E4(&*--54-*-664/3--/BE?-*01006:347?=BD79>0//7-.1.++0*0/+*('('(&,98-'')-+.6+,&'$$((',-,*-1127;8FD<,*102857644513-2+%(%&'&+$+&+)+,-03022BDAA1DG116?9/0?,0)/0,*+))(-&*,()*+,-(*$,51+'*&.0.69(-'('$))(',.0356;65654:AABB=GA4*6*-*%$$%'()*0/<A27B;3521/3-0102>>5/18036=B2..$$)&&(0()%),446:;=6;*).;B>D=?7434495+01,*$(%').-2.-/:;7821:3*8/',/'(*%%%,+-*6?1-;>/3-214681-*+%''12++.-(1..-2621,,)&'07C62/9/013=7-:.5,+1,3,,...7+51'*).,01566A8132384..-1-1+/.)))%%&,-/EFGHD2+-(-%$%(,-1'''',97+)1.()*35+5.5;D.+,.,0034F;0.0/.,-65==D././,21540*,/26BAH=75)*4?59553*,)*-;A/0?-&&'+.**&&'.-/43.18=B,12.53*'--B+*%'&6*8+*'*,-423?:0,+*('(*-3)'++%&%(*,.+-...4/.-0099=7C7,'*)+7-48//68;C;@389;<.6.01B<:9=0-;=16><8)(*&))&00+/,,+*(.-,0/08:34,,+.),')%*)*/,*+.)'',-;..*@?+-<;)&%'$')-)%'*,-/*+('%(*+1;6:63''('('.,))3:3B./.*$)%))3.51.&'&$'$(..45'(0(//1036B;??B50)(*+2$++,)-%3+/,,%,&'%)%+53'0=9:423.17'-(),D446/4-((('()&,-179+3.%.*.)30/0.$(%%+')-,*--*(,2,),)-&$((+4+%)*&,/3D?@4+)2-:8@>A;@IH>4=+*/:1..137-127=;A=931(&/+.0155**,1;/$-&&%%*+4B,51/,B,,7>33407<5*75*44-+)(+/87=03,*(($&($*,&*-)+33+))&))2>=*)&%%*1'90.$21<21./239?1F3228+-%%/+($3:05('&'&(),,?+++B@D?19:;=F/*8?A.1+211620*%+*('%+()++:@*1.,/78@A3*102).1E>+*'$'%)+)*'*,4/>C00,%&'(+*,.7*+*./03-).)646>81A.16.%3&06;6((+(/7(+(*-3D2225.74F;0<7.-=86>-.4/-1<0./-.2.:D7+1216=())(-2827;04<>./-)''++.699,==0..)&$*''%5/846&/50759?()-/34,9-440-052363C5+)0-/1676;5437?68@200-/02:0+,&)*AF5??B?=724.20:6A;GDGI<B2,07/3;<A>@D=3@5006''%--A<6(%%#%//20<66686077/+./697-92/('(&1*(3G;.1432/865(10+65,*20(.)-6232@H40084;D6&-..30,%&)((()10610/341-(&.12-*()+%'+'334<)-(13<<?>EE48A+'*+0096,/1+,1-.,(-<+//,'-+--32,9(1)/()2.,70*,2/+.1'0.111&)((+;9/305782.1*'(/.)6+%)&(/01@@A93274*'2414=619/+%&''-0=-4173@;4;956B-)(&%'(+-./.;@54-0))%*14:<66549DF;1%))(12*()%%'('(4+./)*(++0314-3,)-,-(),0..+,;.(./,2*+%+3-4=H;:91-*-,-/0/30,/+'1#*)**+*'&(01C=;)))/,,-.A>32/.34)0(('12/2;<:=B;,0*()/*,.21/4/7700*,)$)*..-14-/<G=3<B0<+9+4*-5-*/(-(&-13?@4=E:/4+3:)11/-.+//3'1)(.*&0(()%('+*5.+/02*(.22=>=@,.)+-4>>;?H66A7C:=?@>=D:?/-3/@II>1BD;*-7,-0=97/-*)*:<3(;+,%&)25,*41)&$('?A>BDEA96+)*)*-557C=0;8%(&$'',&''.(.,/,(,/:9;>'2281-46879<768:1</'.4390+)-)+'**((('7+,&%&$''/3.)//.20;;8.=C>=<D:<E1.'&$('(''&,/53.27742*-519;:'/..,.0,.,*+<H=7:@BB7523,/*&%&).+,37(3<(.,+0*3?;:8=(+-8-033-+4+54,)8*?10=B,@HG179H=4./+--/4088%*)3;9@5)-1>?774&(00.+-,,+)'(')(+-8*)(((51&,*+*-/00(/,&.)&&*+--72<0B.8??>I=3468=533/-179>;'.0?1G/?CI58B:.--:7.6>>?978A7:62)/@I>8?@78/345H@;523*//2@;567;&**$&*.51?A80../(+'+2+0)/4A,%(,/71+,063,,$-))&9H4@<1103DF7352/,'&%&)&(((+)(-),..4/-019:.>,/'.-04+((*'%'(%*)**$,&3572D22/03245)-:6'*1)/-213*//3089/-217C.-(0018HJ?09:516D2,2.-*'%&)0/3&)3,(+*0/459734/.*.($*,&**.*010)122,-.00%('&&*(((1/*,+132283<<:..:303<)142/*&102+&&%.)-297==020143<646)9662:101HH/,340=;43+,,.883,/+.;:<=@975.47:<9<=22-&-39FF:,'&)/+:-?267<@G=EG2A/2;?-++6CIDC7210237<=56615,+++%8=.40)%))+864<7657<3,2/--2AAA/A..-</3231:88.),),--4.B8)'(%&1254.A@.).-&77/81*$/,=4>=)AG?1+..0+01C@@<?ACIDCHECD:-,--31304/1233+(/&*'/+--.EE>D859?70;*0/3/4::3//17/%&$'2)'((%***0*/:567=E632-+*('()-(*$&)2++/89>DAF1233C1,9-+0=3'*+(,02BC>B>=02./162,()+)(3-0'('0//)675.4;87*-'+%.*(4@2,.03</9/7+)+*;6367-6110604@>:>>?A&,,(<B>6-))'+'.'&*+33-4,-38BA:99</000/?-F,E*/101875:.-/,+*-'*'-,.-.):,/10/6*')6:?52*.,)%)*)))),-;;B/*,**/0,2.2928.,/.0*/-65,3;<H<()(%&"*773A<<=7;::)8424*4=,02)+/>8*(<9*(3<1+.80EC:/3H4CB=4333,//1.'.*&(,3/.6=33''.0/3>@@H<::7.-***)+-/3))-6,+,3(@,GHE51/32CG>>@43421@B310?D*7+22<)%*.,857.)%00(.+-.+41,/).0D/-,C1'**)484//5.//39)/&'%$&+&%&),..11/6))-*(-+'%+/0-4=33+)+)&&&'+1,6<<8../-2?.=C4/0##,/1,/90().///52*('(.<4??/+@+,-.5/003)*.+-?B/475>:12H61/&)-*.))+82,--/)//,1++:2)+4C6*4*(,)),++2000;E9++'+&<<(10.(&&/035@HI749C-+)(+%,.7/'$&,*/)0*$(&$2:A0,00,3,6/@@'>*&)(*,/()++/,1//*(29>++-611*,+)6,/3.><5//596(4(2/1+<F-.1?34FF?<E>48@20443*,9:63--+-23688;83:/-10236<;(((),6+6?-'603+-)-/.)..45683)<*:--.&/'&*/,+++'()++1357-'+'.62/97.+=1-)'*).201/-/.2051-00.2(''/3-.6&&.15D@;6EHG1,@/,7,+CGFB;775048212/B<12+/798-&)&%&*'*()##',014D;3,34+,*$(&,++*4.5?A0<<0DH-3')&.,6)*+9;30)-(,5//+)0+,-//)'.*(&0*/,80)1)*.53&/:+&'')..362+)%'&2674'+0/4+*.:5/-.049:5<6570%'*(&%%%&-,%%('+6460/.7)&)%(%'$/3;,3%)-221E66?:5492:AE;,+$&+.-*)+0%+,=//''+)&*1;:47@51G;11:0//3.12(*$(
@SRR7477814.1.16633 79d8811-9f6b-425a-9671-7067dc000993_Basecall_1D length=5843
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
@SRR7477814.1.24392 33cda2b-7f3a-4134-b20b-15826916de05_Basecall_1D length=11504
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
@SRR7477814.1.22832 c71a776-680c-4002-9d14-e035a1b35576_Basecall_1D length=7804
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
@SRR7477814.1.40117 7b011a1-a191-49c6-9a4f-b866df202249_Basecall_1D length=4989
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
@SRR7477814.1.25882 63f93ce-00c9-4d6e-86d0-a427589268fd_Basecall_1D length=9053
>$'++''%)(&89<B2*(A(')/25:311/0-770-*,+'./<7-.5A=7F57:6/@532:<34B)$&,'()&*+:70/32=02))-,>HF3C9AAA>@B/67.,+*%.-0-00.29A5&',,/;=:,3*-&-)&)()/7.-4&''%&&$%4CC87=<GF=>99;72204D;3510)7.116;FFG/;1./17:58>=<@BGHDFA84&1:4.1?2/(5%')()11.0--/-2=*1(&%'(&(',4370855.42,,+421)+'&%*/6-1<<9;537E:5754;41*4'<11@+),456:A133**'&%(0.'*8-,++%2/(69?;9511073),')(*'%'*#0,)(%%*(,/316;6?55E77781'*-*8)&'&%,,2,&,+94781>GIA7'4,,%+,13)*&''('*/*3'<<3,566@C7%%,,068,-.6--)-*3(+(%(,+/.,>/.,-+;DH99;(39@4*7(((*-.4-36?5-?8F55H6722/2%+37-.76,*,(&))(6<63:@.114?D<4-;)5%0+-&((&484+()*+2%,320+..2744631*,$)+)+$$"'('./3:G,CHH.?)''3*+-?,4.#&)*))4C<,13+:+2866;0>5*/+)&.)')-)/-*7)%(B6,*1-(()$'(&,3+/7F3+-/,16/779:0+*&((,+,08.1(+).++/.,53,-6(0(D8C2+3E,.0=<?20,.0:861'.,0A73<@2,-<2*4.2-.+2(.-3077310+,,)*)020>6/2..,)**)%2..2,,*'//1A@@<43,&'&'&')0-,/35'(&,-17789CD=,7)))09',75*&//036-*,-)*524436..*/$()/</00)&7<GGA:7+-/50,07FD:5)%*()*)((+*)$&*-,/477=7))1/&+.A3@.'$%&(+,%'',(..0/14,)3*C6'0(%$()&23-)&)1<81@009<7772.),)0:G@1--)-%.&(&''7+.:8A8<9<5.#.)1+#$(%'%'&.2/2C'-)',3-3*..4335A586;>@62/./62180/%*&+-A>7*&(,+.-0-/5<810*/,1A6>1-+-)/.(5))0(($),)*<=533-,&+))/.-,%)+*03*+),+01*-./)9<<-++/%'+061&'&&$%"$&$.10(*5134/.,,+-(.,56?83*($%('&#**0-0146E5;E8*0.+',8-&&0157<6,0-*-3,---8;:.<6=1/$%%,')*84<2+-;7:3-*301.'*,03(1*%''&&.,=5/0*5015/+$()%AB>:43.70?.01./21/3-42.6'<5/.144(.(&*/0/9.%*,-:22:69/*73-4(&(33:D+,('&/-+*'%&(()18+2&(%'(.00)'+;,(./.8=3:82488@FE*:;;GAC9H+(*'(7)&-303***&/&)%)/.&-*))&(*,001+/.',(1*#%''&.-,$012%*'$%'')+.0*:1)$&$))**-,-.%''#&%2.&.(((+(*)++23-,'+),.0.27-5)&/34*+().,'.*)/)#$('36)*7<<>1)2+&*C/50-...0--++..41899==*).-)''+&&&'&()-0$26/A41,0007CHF71,'/0.+1365-),6.73.19/(/5&'22->;<94,7))4:2*&0.*7?:4*6'$1&$(*2(/(38+/0@B650.0.)),%(%-)32::B,20*+4,&%&$&&++*6*+6+;0/0?CD/@/-*---4,,+,-+36?&,%%,,+(($%//9=+)*0;C7<?B0..))4.2.)61-/7552;)+750,15BH8;@5.=4>/C--.E,6.2'$(%*)/(47(*65148*('')/,-)*.9-+:;739*'$/%'%,*-,*.-%*.'(&*)004-/-.7.(0../.7.:9633716-+-+)0.#&#((%(&(+(+0.0.(+(&*88(;''&**+-%)&%$&*(*23*:,)/4140/0--*$)%&-)-21),+,.,-()'(()4*)32//.+(*%&$$%&&(..-/55&&'-'97/.0-0AA,0.042(')((-'-%%/55A;=<75989>;?E?==@>++C<:4C+')&)*-*+/00&.'&'36,&),.*--/2-)+/2D=41-,-34@30230:+%&)+&+*,$&),3)+'<;/';6330**/1-+,-1,1.2-('+-((*&,(''**+.-,%+,)'&.+%&),$-'('%)+($$%&28=/--0'.''&$'73;0/9(-+*+'*++''))&+-0)3A55/(*'*11/.04*;8*)27/.02,;,**.'64?<;A=+;1;10+14')/,1)(,*+/9122;+@,73.'*$&'01.273.../+,,*,*,)1++'+(")('99+78.%73):,-1,+7/5,*&)/++.03$&+$"(+)'+,)+%-*/61.@0)+.(//099023:,.+&7;:($"$-*%.*&$'&*)$&#-(&45&''#'#-/028($((#)))'"%21()%&'%"'%&&'$+./&$()(//1+)$$&1,,*/+.+6$%%(($'(.8).%%&)%+().**)')*+'%2:/2($%&%&'+/'2$(3)((*&*+16+3)+.//)))*)//-**%()))+(')*-,/+)((%'#'%%)<356<?,5.+*'))67,,'(**('(*%$((&)$&$%%&&''4=52((++&&$"&)$*5./,,'/$##&)(,&#(+(&$&(/-&''$&'#,1-.7)(-/.063%'&##(%%)&'#**.311.844218,,(&%&'',('-0')%**37A7A<*)*4/200(55100,51-.'*19A427,/,((*',44/@E)6(*+0634336<&*,(((+181-+&$%&)/,$$(*83=5@.,)-$)(.+6/,-.,-1/+)*190/,)3++1+1+-4:3334/-,&&''.815,()(((**+)($'*%)+**6;0,+''*&,,1+(($()%0%'&.,11:0044-,5..*:(3()')&'&&(((641,700*-+*-25-+))$(',,+0,'.*'-&79>*,((*,.()/1*(#(+-,3-7:2+*,++7-7*3/0+*$%%)'/..*.+,+)).3-./110'8*%&()*'*')+*028.A;99*+.0*E,,@/3-*''*())),(2;E=321,'&++02810B311/)-,++*,-><F3:0..-1/6=1,/<BDD=5/',5&$%6/+*()+-&;A370%)%'%&)+85-%(1(**/0'))6*&+:85.32)89:/1'=B09@>)'%($'$##)%'(222;501)%%#$#'&+*,(*@8.1&%%%'3*+1-/,-,-'*%%'*,1+1;A?-+'%&(11%-('.-*&40*56+.-(&,+1/),)($1010*,'*)%&01,'(%'%)*+3<86231%2(($%%&)(/61-.&&$%+475,%1-%'2,5+24/<B*)&%%%).0+,'$-1+('78G8195898=5*7656@A@6GEG-)--)'%&&&-8DF6<>90.:A9694&$-.++)**+,('**4A0*-((*(''3<228?2,**)(/,<,,(++*0**.3*#%../02.-01286.-/*-+9///EDA276@A<55633FC3000-2/)3A-)+)-??<13-)($&*$.$)*-./)('(+,DE=0>+'2(&$..*)*77+2,)'*$%&())*'-+/(2.-,+.>B>6@:5,-@71-7/:56356)+&'(*)6)&7+'2+&-&+##&%'30*&%*-/*$(#-)),@81.77,*&)%&)%*#++5136&&(*/1,)*2-(6BG1?..+*,--6-52::A;<:,-+/--./++.2/<10)$2)051)+*.&%$%'-)(''$&'#'*1-<@?92'-./+;6%*77>5763.*'((*''%%&)2.-*&)3<24','&.;-,,*%&&')*/0+1*()4,&%)0+0217,*(),*&#%&%$%.8:>:-.&'('(61,***16-($,''%**-).;D./9:=/9130@@B-'0-&%'..)&'&$&$$$%%%'%'$&%*)'&'&/0*)+&%&*(4-).--('&&'474(=,+/,&(0?2:''+.'..1--5.//0&0)%$&%&*3<369A><:=->-(+1/,,,5%=?6/*0+;4180+-6((6()(%#'),)():*'(+'/,*;/$#,/6/8745=43/(+,),7:=(-:=1<,0+('*,*&$#$$&-):?:@<48?'3)4.0+&&'&-*'$(($('#',($%$&(45+4)()%&-,)&%%,+.1.**(0%+$))+):5*((+0F=<3+&)()).*33.%##%&9899A776)&'./+)-76*.&*/<276/2*.&&+1+A0*'%%&$'+*%***/$+('(%$#%0.91111:&')$$2,&.//9FJ2<@EBA01)2.&-12--*3(:?=3*'*('%*'*)+&0+%%%++&%',*,6(*&(51//9/,0+)%%&&)&()&'21%#++*'/-%+&(%$)+.989=-3-00.669?(0-754CB90'./+%1&%'(02./;99--%($&$&(%*''89:B?:1')++&%('$%&+&((,&*+&);51/059.C3(+/-2=:::B;9;97::>;<>+1*+$,*'*DA<9338173:@>9),,'+)%-?2490-,&*-3*/1,-$&+,++2.1,)&5++,-%*'*)'''&')*)()*%'&*632'1)2=.117(.2;:6<1**.)'&,-AA@D?:3/00,*/,/131;9/00;20,1322(*+=0((&%$%&5(2326B7AE346-0&,160*11329FEAF@?/),,5611&)0&?*.6)--,-022*5,-/+/2++)$):-AD06()((*0-+,.01@5G9049064,71,%*-.)*&&'$%+,*'/,'*...665749GC*1*'**-83*-(5,)+:;8861*80+588<=69-320$'&(+;17574.0+069/:%.-)59..,1'*,%,57CA@=14<</83.+**)-((++*)/,+.%&&'*,/,).050'*'+),06821459=F9=;<420319;<<><99955&&'','*+./++.3,0,/0;4>45B4/+-1.++')+(3AC.-8/7-/2,)47?8::;48184676741.6034;:87::8>:+++*<23--&*++((.,-+-/()-223221+32+.&'+3)/-2;).*+++,)@-+)'4*)'*(-*-&*,340,,,-CC8C,680+)-@C<53%$'$&&'4.*+*+*((+-%'$'&&%*%(%*+3-134-/)&+877778557;7A.07H8?F/-,2,C++.::<1//.((*9EB;57.(967801.-+'(())))&,(-20-,()/81,2'&'(((*.045191((=+4;<;8233/+'0))+B0/1:>;+/427B2((,&&'')--%*/++,0,4.7016:3,+)**+12/64&6&$%%$$(35D@7D5B+'$%,<-,)24.//++&($$-,1)3+84,.5+*0?<;99.311()&&-+'+,;7@9/0*-)%-,)+)6(((.2*+21*.,.../'(87.2/9;>EA+'050531/145))&%&).///71&''%$&''.+++')())/+))($##$))**+-%&%''*--103:@?@191,/..-01/,()-*0)&%+)(.,,'01-3B332754=<2252)*+)(900.%*)57,(**%)+*3-DC??B/*(.-&'%(+32/'('$%&'//1--++1,.--14'2/344GF0.0+*(())+2--+-<57?0,&)*+//*4,%)*&'/1751G@91*/%,*%,'&-)*31()())04421C3&-*')'&1.,$'*),,)('%5.*'9+2%$%-&),+&&(+953/,+2,(02=9878<B0+(',%'+(*(/8;;5?;85//3)/.9=>@A?/-((+--.,-0)&,%((-37)--%''('&(-(,()*.*@5,(<+''())-%#%%$---*)*)(%%))-1207==2%-)&*4?A</,./,&).1/'$'$$$$+3?6)#.%/?6;0C./'2.)*0'(&&'-',)((,9ACA:.((*$0&23.9/%,+*..-2.&&,/9,1@EA13-/427>,%%$$%,/-+&&%&%(&%+'&)*+)(%./-(&(%$#+*'($$%1*;-/.$)'%))*9-'(31.%$'&(),+'.,))*%')(#$'+3-6/4@;1-,(+*()-('$((+9//,/)(.)(1/3/+.91524,*)+(''2/+'-(.21;*3<0)8%)'+92E73/:1/.-,5/7C<>==:,,+,-03)5;8<>CA-1%+2/+,--&)*.)*(:D275&)059>)2%,$-,'+./=G@;98).1,+('*18582C,)('(-57=A>DAB2A=22)(.3-3173D=*6+25(+1/:16243;CB.331:0.--$*&&)-,'()*0+'(++($')49;9:9;1)***,0/*)3/'+*+--,,&(*(1+&%&*-.'(+,C<ECD;3;32*'+'&33-,%(-+/>762(0-2/,++/6-')4)*-**'(''--*122=2?2:+'$0(.71/)*&'(/'--7'%.10B'4+**')(-,3=/3=1'.28/B0.*B,2(($'&)(&&&88&%').;::87,,-//-**,-7F62/,1/-,.+*))(*)(.4::5,A004-%.-2--(*#%&'9,()1/03;99;5)(5-2/;A4/9;=:0?:,*,7&.1)()##''(-&(#&$+9-8+++&-*)&(%(%-7.2///-54=:01$(&+*(('&%$%&'$(&3034;1=3<A**.7()&'+(*.',1959;7+%(&(0*('&7042671'-*.(.-2643.10+-*,')(&,)%(%0*,/1,))..%&&&'-*%&%).22313-/43;+2-/51/187751/(1/688;/4>/3;<:560)),+(@A;54897/-6>A<8;643-&&($'%+438:;6=6,0'--+0';()*))4+)1+,(%)((-1>.*B02=321>7),(*,10-0-,.&/-,-,,/100,30237--.*-/14*,*'%(*,0-+'-037;;9,(*396<?</**(0011;5=1//+%)0.-16,-0**&()(*($#&&'+@=905-/*,-;50,5557+,1+*()&'%&'((%%%%%(0+**+)1#'917B:60274)919601//1B?BDD:>/,+++,-02-+/.234<75470./+,./+D+),52--),+(2*%%'(?344:D=?356*)&%&(,+'+%+-0;@4-'+.+1.D3(*#,(1*?>A<83;202@?8=57?<:@1-1-+)-+)),+***.*'#(&''01/.,)&):E7<)46;;A==867:6:H>=A'7410,().5212./.<9,-21((-*)1.-3749:>=<-39,,619-(.81>9>5=56>5+',0)8.*+-3*+-,-**18:*.&)'*9344359A8>.-.',3+,%)'$'E,6+('7?C0.'8*66+9.).%(''%'--('&&(-29899;878)',.3))%-*3/7<;9?/23*):;.(+$.5+*)&413E1.6/247B@2-)+)*<,.()$--&5,(-+-57+9,)?65184=7C?C=><<35*).,0:02H@<60/1;F31--+249700570%%&*,9'*%&%7+3,)(&'%5D)8+))(4:<1491.+(02=8-1-,-)*)&%*+*++123/.-6445,2,*'),-71*-'')>>97;6@C/.6//:.=.+*),./DDB210//,6/.2,)1)**8834?'(,)&((*.9/;8<225:2.+-12())+--.***3+5-0&,('))&-02/&,(+/612)1,..-580(&',+$&/7<35/+'001,)(%'/4&-<-*)+)$#&),'&+23,63.--**)+)1..,,+-'301(+)&)),-'((*+03-.**(%+&'$-)*,+2,90//*8*'())('()&.5+4=@%%''&.001:43(+*'((./)%.')00)'+(+'&,+-)*&$'<=9;93./+'($''24937/./-%)&(&((.(1*)+.25760/).1=4,,++*,)-)+..1<0+&'*)'*(++(+'&*+&(*)*'+-..-/+(<.*''(6),.'(.0/.-244)$$)'278511+%'%*$$$$%$&3;92),*,7155>2?:/.')(&%,.59(3//.-,299.','*'$'015/0*,/(3()9*(,')+466/1+.-(&(+(/.;+*.40&)*)(88=*..2-258),2223681.*%*(%'(**22//7@''("$))(%#'*,10?(+&&%+,1/-26)(.)(&'(+'((-211,,)&'(,*.**//4.7/-$((&,(+)%)&&+&'0=+.+*&)/-.&)+,-)2,#(&%'42,%$&*-0<:7>5=64.--640/,'$$&%')'*,'&(-*&((&&((),/9249E?'5'),37/-3194+(&'&&&1+>+92323970;31.+.+2213610%%%$(,((8.00++698-;55(.(;=B<-.1)/000+,-*%$%-())423)*+050+.+*,.-.*)'*5)&&&'/*1432(('+-*,&(2))*)2516<&/'/',($)*-1788)=83+2+;844(--/-)%$*,>0&()'$-/-56(%+/3028408C1*')''*)5)#($(()%&#'('''***)(*+.=-,*+))/)%%$*.57@6/1.00+1595</&8+8,.*'('(&,/+)(+18,0*),5445/-,9'+#%$)*+(-.)/..47:<?C86*((((+,-')/0(-&())49++,')%*))+-0'>1+'+%&%''('&#)%$%((&,.4+/+-/)-*+*($0+507052+'/1.16/>,&1%#%').++))40IB46/,39819.2&)'&'/..1)%+%)#$''*/,+257132(.&''2+&,&''/4%#$&%%%&-/&+37/&&((&2,)(0466D0+01)448,)'&5'-'==;=692/14+'&%&%&%%%&)&&(*0()(-)'%'))0/27331'.3-'+37)./+.,0((.4??35=33+/,,217(?D735+(/((/0%'%100,01+/:@(('@),-3*2*.-*)'2'-).,-12;;EA=@>;6*+)('(5+/3<13(..$)%%$4768879.+-0.&/,(,)*&*-)&)/;A;-+$)/('()&(((4),*7)''$',+(*187'&-0-4))**,*.0,30,-./88;?:;10-*&',,29%7&.(:63=F-.'))(+;8>53--/010/+-((($%%'$$'')(&$(&&
@SRR7477814.1.37530 da27ca8-66ca-4ac5-b808-f584b14183a5_Basecall_1D length=12186
>&'%')(+03@.67C>16<F6511/3:357CEA9=52561:91''('((13.ED4-/25C:45:=93-5+.&*37<46917;B577>;@=C702;3=<A?/0194-0<733-,+,DIE<>>*==:8?=;AF??2=8+1,/,,5..DF:H@?:C=<$)&$(*-68/4@CFD7243./227;;>1GH0.=C=?:A>@E(9:C,/&.*))7;;=?@I20/10;?1D2.---+,.'*4:-,?B:55-*<;8>>6<02331-=/63-/+-*+,4CE7)((/5)./())),%$&%%'(().7'2-)-.28=,-%&%((%*&(&**++'7<,3.;6/80*(+;52//%)(&%&).59A>B74228&*1179D:<>@.?-/C;8FG7-/%11@5.)--/,.((,//,/02425-$)60488711/,-(.--;2?16.-/120027..**.5,'2))&-2458GB4.-/+0.46:@@F06,1-,,+())6969A?@<=>CB<F2=0'/13./.BB11+*)&)+-+060A92224/G//3653A665<>>;87:4<<4289@FI22()))+/011;IH69B<>34;?<1?B<<5.5.,-<-/..+.1,).5<4AF,)5+'8787<>3-&'0)>/@@A5:+/2*''))',65FE;8;7./+1;../0<GD=A8<<?+,-,5562)7276>6988,+0%-2029=>==AC=C14-.251?4990.59:I8(6+0/2,*->.*18/;D95:3;?9012115<=9;<<<C978989FA=98.,1+,((--+.-.:1/6260+51C>984697013.+0/1?A79HF=<H;<:=<*7+(*'&+/,*.;774:*+27/?0:1C>;DF=E108=D<<:14'(*'+$-.7854/.24A3'**,(,()4.,0),02..0AF360,/.*0/0(***.&)%((&)*-4<1-4(.(4(*</,52/4+&&'%1368=I721.A20/--07B<39:38411,0-486<D?.*--8412G=G56,32*22BI=15<5,/3CG:>?.1FII2=5=2-/8=5.*23=3/%**)++04280.((&%'62(')%%'),548;+3026B/67852;?I8675,*)+00724B;/9794<@43012:371E/G+0(/*7)(8B7=5869.7<4=BBIA@@4')0-/.(&.11=923343-789+*,,./4.-,++,/58)/6DBDAAA9GE*-,2377BJI79=1901)47;@FHDECCGGBAF2149*+355<08IJ54860/06,*/9>@;724')%'('(,'(%))057B<IIH0--,*(+)+107.1*+$-'$//%3::<4?400()116+(0'(68>D-06.)0*.%-3+34+4+%')/)5.(+(&*53<D7,;><430=<9,/)&,1,,,-)&./054BB;1123>66:4?>;:8IG:9BB8C7-+%)&00-'-*0343-(,('%$&9;<04=50))()%&(./7877><E8B//;@;HH8>IA*B*'***&**')5$,&))''),=A2.,(02277@9<=:@IC;0-(,.))(:<3:A?A/-,--/*,/12).*,'+;9=<=?145(/-.,2445F3.2-0F,/--*)+17;+;86$(')*23@?E=---+/%*((+1)+)),,)--CF?=<02/12ED,724=/I8E(:8<)2.-,*.++-256:EBAAB613/-0.,32/737E<6;>C242234:=;46;,+1738@F698HID3201-9,9/ECCCABCC@778F.323/.(+(9-.'',()0780.ID/=7&&2+8A5<4CBC7--6359A:>9:3A:?C<7=0B/@0DI6;+,4.,/,,-21;H:A..')&&&+'&*((&,'&&*&.)/0$&')+;:*8*'5.--557.,/$+,(...34567F?:33--37,9+/*,%?ABEHC;+77AE/6A/<I;77481/0;6@HF=E@6,,*'(0-:?8509;>G?:>=5?5D0110)0(/,005;:E:=.>.4-*)*'',01=/4.)-(+2+,0286$;2+11*521)13H355821$)20.6>7&7795)'.0/70())-0:@E?=?=@0DBA?=;BEBFBG@'%,/*++?=+BFCBFIE;<?28%*,00.31=<3'4;-+0088D<?:03-%4+1EC0GI.0@C13*,&'(*)+-15@21/1'*.*;2)**,(+/$-(+-,,>?9681;+B/1-*7615.2;4,(-.2:/4>@&8A.1C98:A*>=15239--(),--%0,3,+66C2-/%(.(05:8508E86;BCCEAE5,5=3,*+.(+72B;BF98;1/0001)1/88=G-*)))./97---.,1592<CA3229;B==>===<1:<7/;A::234?>3-/:;9:13454234/4AF=5601.7,%)%-)$%))0(294(9+88;991;+-.023=7?/547D@3=6/?.0+62,+/+%')**,/:</4'60958681=73'**-'*//42*(*,41.60+,.//+-084:(+7**(*&,,%)12337249;55479GE@?9A6-:-)+-+,E.B/?@%69-3.''*'76@>AA21.-)-.03=+12.()*(*.0/09,5*E9HHGGAF=//;/998<B@94670/,/'+1B@8>93/090;B;18<.0@4@/'753+/:27.(&,)'+78&6,)&)+FCDD(<==8CC:363E,0?.8<9'2@6548<;5?G@1+&00'%&($(8DA56./*56;9>9=>@DC=E26+)$$%&-)*(&&*4,&3('/>,+<?8-/''(+,',5>?C+?*'#%%1)(1--$%%%+24,+''.%0<.+%''#&%&&+/89=3<BAAAEEC:A<6'112-5/93=074://.<5;.A-&/<<%)&7.7*((&&-+0.BA@:<==C2=?(21/-2:@?@9551'()()(*.-1;.A?9<>;1<1,).,-)-'%)*ABFA/.1%-6++4HBEEI4D334((30,+.-.-+-IH2,9(26BC1.+,,;)(@EB-20+-**.38:=>7AGA-<F:5).+9>/-??/=DH?9:;(%??=BAG9?E724=79<-*)+85.000.'.26:>02315B;4-,4,)*,/48?:D?@;711.255831.60.//3/6;=;EA@@2/017:,.G2+%64(*.32((%%,'-1248<<IIG764C01+,446>95CA24DDE/22.+,+-033;GI=>;91@(2*=3100%*++,=E?@@GG;;<9C1//9>;;2.51.01:7G=4.+*&%'5=--3>>897(),*%$$*&'&'#(.0@EG?/=/4*-:E?51223@>B;DD@>=EI1:;87378&'%-,11@31),$())+9A=<@669A<@<D?C?8:24574:31-3&*/0)90-+*+.0//2+0'.9?<FD',179;B6-@C2/4@529/?/,0+;<IH?@AA@6;++)B9045'+..5742355>C6:1'1(,:4/22>A<=:2.14796247;00.07:?/<35;,+.-)-,+,,+5///',8/,3+-0((+',1932,-+/1--*-(,)(*+0/13)).0<<.6*(+*9E9G:6@8;F655+)%&*&&..1.047=@BDI0?2355//)&-0-003A9758:.,//-5+2)>6??@:40959?=?6G=@BIG?CGACECE<<0/,D+-.7;B@=?E:<'.//11-22DH@2?04346FB>79-..0+8,5,:?2,-+.,1*6,)%14%)'.'8)-@B<AICE@C71,,&'1)64C@9=<('28.DH0.9DI>('')*29IH/D?=?2*(''++&)1+8.,7C=:1><3-('(&*+71,0)*1+1/474/*)*8979<GC;27-'%&%'&,1.463-50/70**+''.9291.+41+&%,/..2A0-1101)-,&)8F9I:@A52+-1(05/1456FI4<C2,<B.).A9B3414**./35>>D2+112.6545',+2:+7'9.-.?=D8(424689??<27(44::771/274081868A-'(&(,2./4(@/)-3156-201593:>=0//-.524)/40E*.@6F1+,+-((3*)*-/>=AA53183,&$*%926+.02>4;8:,-,(((&&*/&,&$'(')(&-@/+BI@?F@A>:IC384201)5/',/,1::'6'&+).04.(%A<67.0*4=4'919DG=6,/00('%*-4A,-3EH34F>D99@;5@?2.,'((),469:?2:47F:98898EDJEEH82./5/4?=<@<@>21?>68674)('(/7'+:E4:31,>313952,&,29+.)--1.4*+*.+,(::.2*(+/19::249E?-(&%$'%(/,)950B@59.)()*:9298463/3+-)(8:741/3D.2.3565>FH000*0/03CD<)*)04446;5;/?/%:<8@@74477'4.0?B3--)*+/-*+652-;HG59?,)'+,*/4()(*)(),+-(6+8C6=@A@;+@54F66E@A*.(%%)-,&628D<;;74-/-02-).1775558E--**0+,+.+*.-.%(,A302<FI<:?BAC>?<69765))=B0814/E8DDGD/+-+*)'(&28:=7:.('56/..*(/0.3E.)&-*/.)--1=<?EB@?5(0(),4*>.2**,2.269<%(4/39>12:/*443?846:<5A4(-.-*)/67:=922:2,)286(-//9(646,/4975<A47?.B537/,-->853;8<.-+*3;5?=9=6(/,.?43,,*&'%'%.7GH7,1'&-))&+.;?>9=902/0549A??DA651351,/07)%&&*55/375/+69BA<;6;1+)%&(',0../*4-1ACDBD?7ED00-+-(/('&'04/5/,D=>,1#--035/2*@6**+-*0.-4:1?0(/,)4401/(0&&'(01565?97<;:84-+'2+,((*69:<;84,*.2+5?B,0<7(><<,*/3,>6/+6/+-323..*.2B;,/12216;10A6F@AE>6<>786;:6-&''((,06,.044>2?416;49:8GJE:>;8B16A=?<:<:5&(7FI;/5?'&7>87)0+&+)*-2-'-4),22<7@B/((/088@>5834278/55-3+-024-9.(-98<D53234(:;8<.<5*%*(+-5.=96FH1><--/-.<)?E@EB99:<EA@DBD@>?>=(/++)06.6;0),/.&*7>:87:()%&#))=A>>(-,),392.;2'(-/3>?105/,/*/-/**&(&&$*--880955-*+,.108?E;@++**&<>999HA1.*'%3)/+'((763+)114-&-361*/54)60)0.,)-/0@<BFC*),48/97/063GA115::AEG@D)=<>HFEA5346/5<DE@;@>6<@.765<;>CBAA*(<>.,8-.1:E6;543<EBE8<G?3;879;67-362.,)(0+443*,-.;IC@78651/157;3311356;0367;73255663(*&*+,(&(.,%/3@;>@D.1)2.))'*4./452:(,)<>DC50648383*,2.2-6?66)6<@-1/30>:)02<A93F032)'&%-+-'+',+@FDHJFA@@JHDB@778IJJ28/-**%&&,*?3A2C0<:522=7+*'-300:/B,57EH?713/0217FE=3'.23?9?03>@<423/5C>;8.03?29:4?513:=35720+(-..+00458941.018?BE=50*),0.3456/4<.27.../2445?425@B9753C815-869FGAA>@?5;A9.(*,.;9(6.'+17==8;987CE:9>7.%)..3/,=D.08837.38>FGC5;@68E1.<0&,/*&''.106(2316653;JD<38<964;3@45687EH::420332A<66:FGIA40,/**::,****49?E>86-+'''108FFJCE5123.0?3GA6-.7432/'/.3>=I5:8108<@EF0*+-117BE>D9F764A5G61+(+-9@;A01=84899@3691,0>C6.02'68G<'95&)225355539?@934=8:6HDIJI.=;66@IIJ<5'')0CB@->838(+..*-<85+&'6567:64,.<3:>=AA31023BE@>AJD9E.7-+-<5C5CE1022,-16.)(,389AD?G?<>BCI1000-;ICEDBBDDD===:=A6669:HD93=<?D?4=9649I@06G7;F:.&*')+,)+-00),%*79.91,.23+/,10,--,.+/BBA,)*-,0/810*0.;+=JHD/<;<;4%00,//*-+%-014//1?EE=14,+/AA050+91/4=>G?CCBD81125<>:871/2'04;0<544C@;A++0>H54)*+0@39.:20.26;.12:'):.-*+.*024(/'.-3*D8*)4A4<09264437,.+**-*,5>.-<A?:;G:9=B:>,703113*)2,-*),;(-(0**+:7;218@86*),509B,.?????EGF;IG6=,%%'#,/;+59'=.3>$*((+8C<;;@@@10+)'++56>468.+')+/:64)2,':?80&('.9:;@.:-,,E+3?;D6;2@@5DFI04@H8B58F846<,+),-;9/1&(.2=;;C=:7,67(59>-BC;++##,&2(*400,/1533311?8,),&')+..@G?+()('/,/-+.9BH3<111774:7:;9286*10..45/((3235,**,0('))*&045>485271+032/.*9.,(%.%%*('#%'06(#$''&(%.++)**,254(;7782?005FF8,/).5...251424/8=E:/*&),-(3'$/)/3E@9<?@>CE8232,)+-,/-5/),)/-D324'1602>:=?A<I881.0.,11.+./=200---//*.1B4595'(/466,)01::AED,9+827E<:;4-(*()(+)*,.0-,-25-3,0/88//0+?,*,+-,*(),//.-*+/03<=8>7.08<E;11()%&,55=>:C.4,,+=?H631/1?=HB86)2+5;'2-.-9==:</+-..25.;<5;DB/5(18<28HB=?<;:9<3'0,13A@IBF@C@B@6-45-,('$,'7C77D><<:;+>0.4/7797?A4132,/,4336D>86-47D5792.-,7478:990*15111.16=D3.()+/=:=AD7.0-,*).55=..10).8/2.)+26@=@2GA0+857&'-*//.)0+6;>*1339;0123003B:7:6+,591DE</5:56469/84;12@:4:=G971++-3:/7D1',///)))'0:(3B54/35>>?.805--7:?:477:<99307,(-01:;,1/02.827-))44AIFECD::0(&&&&'$''*<<93'9A<687,).1353-6=*)($&$+93=??=?-=)+)3/3/90/00+')(=D://)()('-36;B<+,$)'&5.=:962:<2;>@EEC@>B;/+)*=D@0?@@.9/B2-37?<3=:7(8;B;3%('+-03/)9(298D,4110:=DE:774051223-7807/34>?B=78677((020'%)$$&47?>FICB=B<<?B?7-.11214&0))*,-'&&*>.&(*)//457344*2/.26')**0.*+&**)&:34**,-')+99F=9;9=AD2=213+'(02235466:F@:8(;;+()/2'(*&(+)(;-)++%*/33/01<7-(*&(&$&%&-=:DBFEDH27H969B3(05+)@;1682//50-5:7E?9-653<453@4587659;3EF11A?BD=A:025((()%)/.27<>:698F=711002<9938,446+;7=B..-.2701,%%,*04AA./668@@.@/+/(%%--32.+)1-=;87<883='&-(*67>6:IH731.0:;B;@@AF50.,114;:*()(,..69E32--,35;@D@682245<6<B;<5:<06A95:;E+(',(;1IDE:1:10+).+,+;7('%+*-246725>:@@EAC+4-5-&'&(-/?9?1/++0>@HA3/223A1E/0+B>=2@23.0'.*199HE1199<BCDDEDC>=%1(55*50?579-5:B??AA0/32.*+D6?50::11::47=8;;.38304499@=>7:?3:;85*39D>6;,,0DEBFEB<0403?C:-08++/-+-2/534=083142(,*-0233+//459>:D27C7631,-(<A@;58C74354C>/9E?0A?5374/2;;=A>>??02,0.',,4:G6-/0,B1-)+46;AE774=839<;CB7740,056:<;78+-;DA:3;4@28<F;=/1.//6;B&>'''+*&/3/48>?>?<8705*+,51(0-/2.-,1/8@91(1693>0-,%(.*++46765>4,3<1?+2+)(+.2125B700799<37CD?:FDC<&>B42BF556>4220'(('(+.;EC12&'5;636698:5654,-*25G=DIF=AH86<:<<B/+4%(')*0':;;=403./0/,236'5023@@44-25C))(*-.0E9776,=.+-1356D3*+-&*)'),4=>987293(.3;>8(2+)-)+//061>=>@<GHAAH45>G??7/37;6.1=8C9831.0235778DEH/217791427.5B>69B?C==809./30209;:?F?=>6<57686?AA-(.')-*.5,7:/3<.C<--CG617**'(*.,+/:C4.00<3)**,35*,.2+61BD@86%'8A760?2CHD)(280.4*373+65,(%-,23>=:CD40(4).+&*.C;750,.--7922;/1.358;@-,,4=;9:7.+2,-60A22B<4/*54589:8(4032:35,3-;5065:543,--3AE<>>00.*)-+0'*)G>?<-8-.0&)+-4B501'(,8-/1.2?653320001>D@>8)++-&B9'9/93@,:>C88</;9?@0*2./38=0/21+*,.2.&%(,.0236:C@@@5.110;+,<AE87621141+*)()&).(&5C45335:E8988=61%&')003/;='27+)*(+=??B34.,22*/'*.0@??A59917,;--C/..66CHC7<<9BI.0),3969EH8>HI;0339?0/1-3EGA4243@E:42879@)),/2B<525?EEB2@=3)66DE:./&+'*-)(+2&&'-*2(*''(./40/(=),-3./6>?2258I8B;)'2)()&++,4:5<1/*1>0+,*/3+54C/44;@4/502:;><-9+,+=9-+','$$+.+(&',8788<?<C>3:6216/<:>.-).21;;D;CG0./9I*29<71%,,;/:*)+/3:@@;CJF;705)))))4;=81IA46D;.9*,(*-20,(/7**'4<011E6/+-/,,,..69@ID:D::<G?G,+-+,;9<9)44*&--+.-1(*(,.<4823-:18>68;HC@=7=?9BD>F62)-1*+,--56681G0.,'.*$*%'851,0&6;;=B31.)08/00-),62%')3598B41>2<DIHE;.387776*852*.212;<7//-'+*+0389.84**,4.665300=B/+))&*49;BA?43%-202<>=;>/?F-->=>9?,<))CE;330.)*+(-*-.51.3755442<6(+,,6371)-/-22'90),%$*6.2/1/GIF4BDDF7/022*'&'&2>:4>424051627:6?BCG>D5:;>78:C8<>40,'(+52,,-'*8E9/6(()45;AB<<-('-$'03:HI2D566:60*)/*&))((.A012/'%-<H33,,2-+)-+/46==GDF>=5>A;<<?8=E5,(,0155+&,1-456;E;,1223/15-7)16..-G+DC;H./,,,>?DFB<3/((,;91?,1-9;691334:=B<8<28;/1=<?4F255B:@>HI39010../205=8?143,/4820)341<*-%<./=+*'-,.091::<:9<:46:>866FI@.0<513>-0/0219DD:1:CFCC?C.3%8')*,.<<ACBCF?;02FE-*$&'(&$&&%%%&('-C<AGA,*)0699>.)*(4CC1->/.<?**(+-)6-.8<6779%5'&.+-52@8GI@5C,*)2*3D5C332*+)&5360;A@E75-*(')*:GF$&()((*+,)-0&%+,$+''*%*%'%()#%'*-(#$+$((%&+.+*,,*$&+.,,.'%(&)$#&%&&%$$%%#)+%"&*&*'(&'$$',,(&%%(&(&'&'&$(&$%))2*(4*+&$&'%/,+/%$&&)-&('+$*&&'(('&'$%'(%(&0'(&$'2'(*'&'&)+:9,(*,,$$+,,.),4++.0*%))'&'%*(&'$(&#&%+)','%&%#'#''3++*.'%$%$$*(%$&%$%)(26)($"$'(()&#%&(.'&'%$&&%*1('.9/1,%(%)*.,'&&%*(%*1/*&%&%-%*&+)'),'$&,),-2)#%''(*%&*/(.+()(-.)+*$$6&$(+)(*)-%((%&&('+,'13/)'''$-)''%%%'&&'&&)220''$))))'#$%&"'((.,,,1*#$$'%3,1*)'&*#/'+)*++')**//)),)+)%(')#)))&)(.)/)1,0('(/+*)/495231/2)'(/(%#+.6,(&#('%$(&&&-+(0/'/''*(0.*&,+/,+$)('$%&&$))'54/+1/%&(('')0)*&)&'20(+$)2(*-13/1*'(&(,'&'**%&#'%&$'(&$,'%&),'*'()&('(&$'('#''*)(&$('&*'+'(($&"&%$'(%+1+%)/&$%%'+/)'***/**.)''()&&"&+.%--&(%##%)+*0/)-,(''))%(&(&.*(%$(&+1($&('2/)#%#&%%)((#%&&-0'''$'#&&$*$*&&./+'+.-1'2%.6+0;;+,/7.&*+($$#$%'%+.'')'%'&':')&"%,+&'',)''.,*0)$(+)#)'*)#((+((#()*+-*$&(-,&'$&/,-/0)'--12)#))*000-+&$$(*'-'%('',,(/(()%$(&$(&)-,74)&-.+(&&&($%(%$$%(%&%,))*#'(%'('&'((%&(&%(,%&&$&*'&#'#&$'&',($2/.('$'%*&$$%#%&'.1+(%&)(&%&%*+(-+-('-)&*))2+'%(,)$'$(',)%*',')$+'-)#%/()*1240-..*'*%1*75)+,&01%-'.1,-.36(''''%-2172+-&&$(''%(','$($'*+5)')&,(-%&+*'/2.''.:=<:2*&$%$%''%%&'*(*,12.$,&"')&&&+'.(%)&'11-&*('()+('&''','+*('/0&%&%#''&$%#$#%)+)()+-,,-+()*(,+&,$'#',((),)&(*(&'''%%''&%-,$*),)*+,&&'&)/0%))&&'%,'*%'484131/'(5*)&.'%*+',+(#)-*/(((%%$'+.%$$$%$'&&',,%'%&(%&$*'#%(0(,2:-*+)%($$$$&%(0/07/'%)(&'*+*,/'-+104?.)#0+*')('$$)&%+-'&132'%(*0:/351+2,)(+%((''*0((+)&+(#'&$'+%+/'-*)-&')&&($,0)&'$#%%%'%'**#&*'/+%('.)'%&%#%)%%&&#&)(*$(+'(+$*$(%&&0+&'%&$'#&#%&&))(./+(()&)*$&%$('&'&,../5),&3*)&.'(&%*),+)%))&&#&'$#'(&.)$&)%%(-,*%&)*-(*-%,&),('-'*)($..1.%%)'.6.0$'$(&'''*/%($%%$)%+*)()))(&*'%$')-0)(&''%(*+$+'''$$&%$&$*/)#&%)%$%&%)*&&$(#%%%$%'0,0'$$&%+%%%+*)((&&%%*(.&+($+)(.'&)&,.,('&'+/1+,)')&%+,))0-%&$&$%&$%')0-)(.()/-(.1(&)*1+*&%&$)/)')%%'$%$&)*&$-*&''(&%%'#%$*&&&#&&$''(%%$&,'&)&,%%*5+.*--))%+(+')'('%%%/.)&&&&%)(0)+$&(*,741*&41)')''.4)(&')%$%$+'$&)-0'*(&(,&'2,&*'.(&*,+4-.*-)(''$$$%*5(&%$',''&#%&&%'6(&(%&&)$)**&((+'()'#)&&%,+'('%(+46+5),%*+(**(+$$)$+5+'%++*+30'$,0138=-1.*,+-7',*"'%%&%+*('#&$%.,((#%%*-*&')*,-)()/*&''%*3,,+*,1(&*%)&*)()/(,4+0/+$'($'++(,,%%'%(*#&%#()')(/52.(##"&,)'%'++)$'%%%$&*&.$"&$%(#(*''%$&&%&$*'#),&')%&('$)*'%),(('&&*01663,$)($3-*'%(0)'%8*$(,(*&(''%%$)'+*..+,/*7,,/&&(+&0'((*&')(',+#*)&+&),5/3<1-..'),*)&)*+&%&%)('+./+)-)56)'05+-$%%&(""##$/)&)(&'&
@SRR7477814.1.22307 219c6ca-de56-424e-8dda-9baf4867577f_Basecall_1D length=5876
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
@SRR7477814.1.16822 b4e5887-8991-428d-aed5-d2039dbe20e7_Basecall_1D length=19237
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
@SRR7477814.1.23664 0c5b47b-9237-45f7-9286-cea7f82406c8_Basecall_1D length=6497
>%&'$)$&()':;;8(,:,*+45.=1.071+)*/././-8--'/640;>.353DC.+''--4;@88<)A,.+%''(2;:<@D2BIG?82(&($)(#(?5@++663CGC0%22&*%)(%02,().1/<BB475?-8EA/+*+**+&),/5987E:55?/B<2234*+.*',)(*-),0156,+*$;3.38439?HH6EF0,+4/102-4)&'%'*)3(*&*)<::1*(**,-)),+*435126E>:998@?=>=B7DA432680E332C6FI42043:9C8:DE0DG5B4119.-@@:-+99)(8678.)&(&-('$'')&&,++)0/*('+&,1>>3940"1+?DF/181;&@43:A3,114057?13BD1:;6)3//7<:'&&-*-..(*5;@7*,'+*()-.5><1.1&*'*,0%15,+6<B*68==@AC+-B,+(:(&'&#*,0.5,-),.85<-50/-9:6(*')&%)#((<:?BB@8=4*>D64;.05>31<5-'(*-):B357@603-..-.'+.//1.*.95A63>.34;@H@=*<><=:+(+%./,+))+*-)(*)+,)''*+0039/42@?.-()'*2%(003>96569<05A9:4DD3'+=D61114549D2)&&)-34.93+1+4031:71>/.-03(2/8*'/*-:5F@/'(''%%$'&32:9?B21,2=067A6-).11..+'1=37<@?;>A-8<:1@77A/2*,$$*/13.*-)/*2+,>,?.7@D;HF94;+-#($147<66616899;87783A-++()4%)8FI.<=300-0/2=BCB:-/==8;5/+*0,-.B/:=1-33.59-%$%-728F@/<82=DC/,.'+/425.(*%,),,*47<.-/E<<:::?2500/*/+())->5345/,1*+.=E8;0.')0367==>BCEB<78597<=BE@>F3/59A27B?24/:=91=>/)&-'(&'%)+*+&,,.070<//-3(*(55?<?9AC9=JBBA84C3051/))(4;667=>F6==538?.EF:/6832.0/>2,*'('1);A?/-.%0=?E56007+-*./,1/5-&*/:H?;6:60,/,1*+%0'/4?ECBBA363/6216;<00=6:,-.11;667-74.1B24-+4'$',82;AE7(66,-0.5+3*5((*5-0..0)053*-,#&+3ACGF@=;.19+/':C=;7449::9/40+*(0;A,7461569>A/(0+)98G@B@0./01;?B'>-.4A@&$&).211:9;9-+*'))%&()-;:<;9;17B2E3<2>@=22).-4:24,''(0:40147>A34789;7/0/9127=><:98:.4/)*1.=3,-4;7,-&-'(&('')+/22/8??644:6*/,BCGD8A.),.5/,)+,02,+/'+(,**')(%&'%%%,-BC99?../751&%?DB9D32275''3<5+3()0+)2+)*+/,%(&-')*'%+6--(*).0)+/35F36//19A??B4/00.00B-)+-3)*-+.++)7F3=5+(.44B@@II6:,/F:;74,0*,,45A<4649E805+/?CE;7:677<B37;9784?8*3,08.;9FCDE3AGD15,*,/<:;6084;1@66744205,(1/0)0)+0-99@?3@.))-,=36?@E78852752384%(38:@F=H:9:;27+1+((,.'+.640+%(*.810.,.1488B.EHG070(*&'*&1*-.5'(*0'(+.$(5799;A@B6CGB9G999GBDEDABA-0*&$.,/12/*+(++38=@EC?@@;53342466;36353=51=990.,,/=>ADFB@9<;0,'(.0.'/+3501340++6@6254.7=301/1,*&*757:89>BBBE?5/>D*,-928037;8<912768731110779))0@CDJD.,(*.,.8'./%4(&)3678C4544>+;>511.,/46;;:43/).*)'$*&(*../00/4;8:9;C?B43+-+/34/&'*//-14/22:633<681;E,*-BE8H655@=9<@D0E.;/28B<68670>79;://-26;<>@F6@2--()&**)09H=AA>>55;&.*)7?:C>0:27G:4462'.7689C@4/--*.033530/+/,0,:+10?9<3*.,1*,053,51'2))37$.)0-42?E6/))'$&&%%-+-'-1,+--05732*.()(,&'(+(056-:*3)77.5/,-7,80(,),,/79:?>>>.+(2,<>B:,878:GEC9+1/.8.-/7-4)8.-*)).157GD::(5*1==><<F31)+&-4'..<+.&,78>553058+.&-,(*.+1,%$$&'(4()',,-275>5/%*&+-<FG>56*21,4,=--4<B?B0BF82/2..0/-E,/7..219?2F;/.=-66)&'&(+=19:-.))%)8E48=@C8,+,,6066@B;;<=E<<=D.-'1+,11/'),)3/4312=A;/-3,,-=-'#-)66.+6<=4B;D>BFE64.41+,3-+*(,,2+*))*'11-560.+1('(),)-*2,/'&-)9?9.90)/3D@'320/,252*+.:88>54-++),-+)'&*583:653,/+(/,>.<?197<@59<B>7:3<52**-+,-1562><-DH-,=E-092.//9E;:;IE34)-6=B=-+4C=),1-68A--,7,E@@.//5.408-3)/694<1>+&4=220894?EE967/5*<7HF80/1744'3*(-04>@?)((*>A>4=HC5401:;+7/2F=0,,/,*@B?<<1CB8-GGHGECA4:<@;98+/42;0436<9<AF<22..1-3:F@26,*(0+=0:)+-1.*&'+..0-;1/0.629A@942%(4721214BB0*,+'-+),,2.-3+0-)+0AA47=8.'',<FF2CF22=,6676;<?@C79.95>B91,1.(.+/.//+8/>-3556&,()0;BB1,6-01,0.232215D79BCAHBH<C?=<6'-0/9'+).1.1146?EGAB8<@777:9.,-..E822/3454+1,0.,('.-2.0.,2-4,=976<;63(43,(.3<5757539/)&++,+--(:5/428070(60,250%11(99?9920024>.A6B2A>;;,9(002*%1425/3'63+)((0/0C935=>:A<630/.(*)(++65/4D<;44*.,../1=@C=2A01/:574A>5***()5-$'%0)+-14,+'/046,'&,,('73>A5::0()0,5321.-1)(,%&+)%(98?-390+:::<<>=@>@>:E34++-)*&+2-01.+/11.25?E??A=;*).315:8346:2--,15-)0)-+6-1-3D:1,,*,0-(,,-+9-5.-3>5875C24.90/.;3?3<=?A</.))+.(**204944.2222==/22,44<9A>:3/.'-(0).00.8,B--,CD655449=<(.'&$'/,-*/**%,*)-7&14:7CBC>8)7<.*-)03E93<0/./3E,=9@('+%*9:<3('*)(,1&&(/1>;720+6*-::7*965=G=A?>7.'&(,+5+0-9-$+41/@9B86&*.4--+:,1335;GF;D=<=@F=@4((),>@1E03GED8B=>163.0-.-0188?>?C875(2,<46/3+;+.11&7,-/2*436765>E8B@@>545458<ECB?==>=?:E219D733::E<03G2-D42-%'372::3;:)04IIGF?A0?'+.10*45,6=,+),34:;B=>/.*+.-.09<B:()),.'&)/*%).&-,+7-.6','(.**'/+++120<6D=B544.9.(/&%&'))-277-'+()8;)'(''0+'/-73,*&&+(44-9>:(00/-+046;9:?8;C:@>>(*(,*1<10+-+5.*)+-)64110+/,.'('&,)&&%&')&'(,(-%146G95A??2-3'/&1)96)9=>FA</2.480))17/4)-40./+0>:36595+)-CDDA<;7+(,.529379E<7=777@87/32+,8;/*72@506425/*),/6(1*06;.:?8;?G?//4032<=?257??3%((531-/,-3=87:7>%/'18:77689?H70((**7=7<CHC))$)-A=::8D70,&0098A*;,.B881,(*,&+2::1-(,*B4618+($&%(+%/-*6/02=3A24()'-.24.54&8...4G@61877573-0/1./<A?DDGGB.;,,-2934+(.-0*1-0>AD62,027>90-414305-#-,1BDDEEE<G877148.--()..;;B=1/,&+57HA?=;;'-/=,%152@413;4=434A<:;9=E20+./<??2;7:.3//1H>:;>C496F:,+=:;900@FCC>8>8-=8,,)/4..*957.-.2=42323@<105-+-/0'(+./5:70+(-6H@BBB;7&$%&%&&#&$(&&&&&2..,(*,/:9D632?22DB550//035?A35+8*&'*3D<3,2(/4104-/-3-2%'&'(,-/'+'7/601/.&3)53<5/:/--/,03;*+,(,@?3-32.3*2*%*%+(()5769601399660-((+%$,)'-/A<6+&'-/77?9.+,86.5-5?86<7()/8*+''@/@<56:;8:<=;9;-9/*2.+6:467:6*90-+&119D9>3*0--2/*)04'2,+&*6:5712*+&(,;=A/*/;123:;2.5.)710()-94*13621102::./0*4*26:<<8H?*121256**+0B@756>836973'60*&&%(().014<@?67*+2>@9:0+%)*62E2282;358A8@:/6603-,*+0;.-'4*2++-'.*0*'//.0>+,/15=3;40/./+.,,*.019A2>6()'%'3)-)&'05:=:251%(.,/'((&*,,',5(//-+++*&(*.(*)*&=333/+...*=88?@9'6*(,6?6,/1-34=039D=2248?(;)*'#',146;HAAEC>/93172:/412:0-.0+),.)*-::FDB?/23+/50<7/,($&%)*9:436(./,98061.233A893/8<;;?47?361100.;?03B?@76=9F>182/0=9>2.)+)*3(,,&.*,-?<58A2..8/-411''#/1526<:457<=@B:>*A26@73--/86.1-&.7?294(),-&,-21295<226,*.-&(&$%'(.12:56@=0CEEB2E8)-6:@=><D6@2;88?(>DDAC75:87/-++$0/11.'+(,1'6(&,,,/,4;5(+)+<21/.-/.9:C?><H=0003G720)-1%2'<.37.-*+.10=5+*2(<')+(+*.73;6A.,.45EDDC:<A2114>?DFFC>3E12159-042IH340=+*781/0*-(-,3?@(23.,)5);,2)$%)+)8:32.8.,&/(+)/%-186>(.&*.0;4/2E<I<<H<A;;80:75::1H0I.*0+-<<?*'/0=4***)&+010)+&$'/3@2?(%%$$)*0135=<=?@2??7<26=:>73-++1//(+..,0&*))49((13=9(%.-,2*4676.<>,,'))++,,*(8;312+2?BCDE@DAD.A;*01=85+@(>0427<642*()*100-/))-)+/6?''%&%-0+,)/3?@>4?2*616765?1025.(18DE>36023:530,21;8;<A5413D:?89@D.,),)110+,,(&00.+..-376<1/,+(&.15:@:@2E8<D?EH6@821/55<<8931121G12,+->:CB@F>9.+-;/017132:<;6:.9>?20A)-.323202?8/89.)&'+0(($)*)>;&(*+53(%+)((((*)&/2FG*1.()-+1('.27<..,'**->-1(.')+&+-+&25+)392B3GCIGDBGH>)7202*,,..-26(,/).*'()-79./-.-/1(-.+)/320172@8520'01/*5)+.-/1/.6**:/..)&)6145656274-96+*4?;883)28:;12-1A4.C+/&*.*/';((313113:9/9.805&4++5FHD?<)*19.-%()&*86678?5>?@>??@>=>:&,049.*1'-*.-2,2:2&*$*267=>5BJ>--1>?:87()%.,+(&((.*.0<>@7;+),+'./6,,(4+4132&++09;101::>;D70-0,12F44.-++**'#*30/3).11//+*,++-276,+)01,+/+/>:?C<E1+)+3EHDB31/(0()+3'),74>CCB310+)+*)-95760)7:3AEA;7;40/-*(+5621.,,48I2,')(,;D?::0-))%&*579?>DE336(+(20),))'1415//+((+<9)*''%(,(+((-88.5//7.'')'*+%/:<B<@;.2,+,'(%&+(1/3-=).+==D@5<@DE/-'+).297*0(.0-3:79:-496EIFCGEDDE0212/9<>,/)/,+91)4.503923&-&&)4;6=89A8854)()3/++2.4>7;ACA?:9477?<?B69C6/323322/3--672)',),/3>6207478>:;87(-<.+&167>>>;=?@9<91<'((?F99:@>-H+**>;.2:;?@<>96-.2:<=EE)--,6C2:1*$&((()$(*.+2%&.-8092/-$$&*124=;630)+%''$&
@SRR7477814.1.3095 329f1bf-ef5e-4ca9-a10f-c561b5a3f03d_Basecall_1D length=5907
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