annotate ngsutils/support/__init__.py @ 0:4e4e4093d65d draft

planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ngsutils commit 09194687c74a424732f8b0c017cbb942aad89068
author iuc
date Wed, 11 Nov 2015 13:04:07 -0500
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children 7a68005de299
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4e4e4093d65d planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ngsutils commit 09194687c74a424732f8b0c017cbb942aad89068
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1 import collections
4e4e4093d65d planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ngsutils commit 09194687c74a424732f8b0c017cbb942aad89068
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2 import gzip
4e4e4093d65d planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ngsutils commit 09194687c74a424732f8b0c017cbb942aad89068
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3 import os
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4 import sys
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5 import re
4e4e4093d65d planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ngsutils commit 09194687c74a424732f8b0c017cbb942aad89068
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6 try:
4e4e4093d65d planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ngsutils commit 09194687c74a424732f8b0c017cbb942aad89068
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7 from eta import ETA
4e4e4093d65d planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ngsutils commit 09194687c74a424732f8b0c017cbb942aad89068
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8 except:
4e4e4093d65d planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ngsutils commit 09194687c74a424732f8b0c017cbb942aad89068
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9 pass
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10
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11 class FASTARead(collections.namedtuple('FASTARecord', 'name comment seq')):
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12 def __repr__(self):
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13 if self.comment:
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14 return '>%s %s\n%s\n' % (self.name, self.comment, self.seq)
4e4e4093d65d planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ngsutils commit 09194687c74a424732f8b0c017cbb942aad89068
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15 return '>%s\n%s\n' % (self.name, self.seq)
4e4e4093d65d planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ngsutils commit 09194687c74a424732f8b0c017cbb942aad89068
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16
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17 def subseq(self, start, end, comment=None):
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18 if self.comment:
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19 comment = '%s %s' % (self.comment, comment)
4e4e4093d65d planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ngsutils commit 09194687c74a424732f8b0c017cbb942aad89068
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20
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21 return FASTARead(self.name, comment, self.seq[start:end])
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22
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23 def clone(self, name=None, comment=None, seq=None):
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24 n = name if name else self.name
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25 c = comment if comment else self.comment
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26 s = seq if seq else self.seq
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27
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28 return FASTARead(n, c, s)
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29
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30 def write(self, out):
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31 out.write(repr(self))
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32
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33
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34 class FASTA(object):
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35 def __init__(self, fname=None, fileobj=None, qual=False):
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36 self.fname = fname
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37 self.qual = qual
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38 if fileobj:
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39 self.fileobj = fileobj
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40 else:
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41 if self.fname == '-':
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42 self.fileobj = sys.stdin
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43 elif self.fname[-3:] == '.gz' or self.fname[-4:] == '.bgz':
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44 self.fileobj = gzip.open(os.path.expanduser(self.fname))
4e4e4093d65d planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ngsutils commit 09194687c74a424732f8b0c017cbb942aad89068
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45 else:
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46 self.fileobj = open(os.path.expanduser(self.fname))
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47
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48 if not self.fileobj:
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49 raise ValueError("Missing valid filename or fileobj")
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50
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51 def close(self):
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52 if self.fileobj != sys.stdout:
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53 self.fileobj.close()
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54
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55 def tell(self):
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56 # always relative to uncompressed...
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57 return self.fileobj.tell()
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58
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59 def seek(self, pos, whence=0):
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60 self.fileobj.seek(pos, whence)
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61
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62 def fetch(self, quiet=False):
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63 name = ''
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64 comment = ''
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65 seq = ''
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66
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67 if not quiet and self.fname and self.fname != '-':
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68 eta = ETA(os.stat(self.fname).st_size, fileobj=self.fileobj)
4e4e4093d65d planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ngsutils commit 09194687c74a424732f8b0c017cbb942aad89068
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69 else:
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70 eta = None
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71
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72 for line in self.fileobj:
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73 line = line.strip()
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74 if not line:
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75 continue
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76 if line[0] == '#':
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77 continue
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78
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79 if line[0] == '>':
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80 if name and seq:
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81 if eta:
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82 eta.print_status(extra=name)
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83 yield FASTARead(name, comment, seq)
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84
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85 spl = re.split(r'[ \t]', line[1:], maxsplit=1)
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86 name = spl[0]
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87 if len(spl) > 1:
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88 comment = spl[1]
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89 else:
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90 comment = ''
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91 seq = ''
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92
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93 else:
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94 if self.qual:
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95 seq = seq + ' ' + line
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96 else:
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97 seq += line
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98
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99 if name and seq:
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100 if eta:
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101 eta.print_status(extra=name)
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102 yield FASTARead(name, comment, seq)
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103
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104 if eta:
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105 eta.done()
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106
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107
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108 def gzip_reader(fname, quiet=False, callback=None, done_callback=None, fileobj=None):
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109 if fileobj:
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110 f = fileobj
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111 elif fname == '-':
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112 f = sys.stdin
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113 elif fname[-3:] == '.gz' or fname[-4:] == '.bgz':
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114 f = gzip.open(os.path.expanduser(fname))
4e4e4093d65d planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ngsutils commit 09194687c74a424732f8b0c017cbb942aad89068
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115 else:
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116 f = open(os.path.expanduser(fname))
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117
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118 if quiet or fname == '-':
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119 eta = None
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120 else:
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121 eta = ETA(os.stat(fname).st_size, fileobj=f)
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122
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123 for line in f:
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124 if eta:
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125 if callback:
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iuc
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126 extra = callback()
4e4e4093d65d planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ngsutils commit 09194687c74a424732f8b0c017cbb942aad89068
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127 else:
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128 extra = ''
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129
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130 eta.print_status(extra=extra)
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131 yield line
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132
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133 if done_callback and done_callback():
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134 break
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135
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136 if f != sys.stdin:
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iuc
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137 f.close()
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138
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139 if eta:
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iuc
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140 eta.done()
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141
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142
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143 class Symbolize(object):
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144 'Converts strings to symbols - basically a cache of strings'
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145 def __init__(self):
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146 self.__cache = {}
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147
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148 def __getitem__(self, k):
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149 if not k in self.__cache:
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150 self.__cache[k] = k
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151
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152 return self.__cache[k]
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153
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154 symbols = Symbolize()
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155
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156 _compliments = {
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157 'a': 't',
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158 'A': 'T',
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159 'c': 'g',
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160 'C': 'G',
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161 'g': 'c',
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162 'G': 'C',
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163 't': 'a',
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164 'T': 'A',
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165 'n': 'n',
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166 'N': 'N'
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167 }
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168
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169
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170 def revcomp(seq):
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171 '''
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172 >>> revcomp('ATCGatcg')
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173 'cgatCGAT'
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174 '''
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175 ret = []
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176
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177 for s in seq:
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178 ret.append(_compliments[s])
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179
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180 ret.reverse()
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181 return ''.join(ret)
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182
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183
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184 class Counts(object):
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185 '''
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186 Setup simple binning. Bins are continuous 0->max. Values are added to
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187 bins and then means / distributions can be calculated.
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188 '''
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189 def __init__(self):
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190 self.bins = []
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191
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192 def add(self, val):
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193 while len(self.bins) <= val:
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194 self.bins.append(0)
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195 self.bins[val] += 1
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196
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197 def mean(self):
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198 acc = 0
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199 count = 0
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200
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201 for i, val in enumerate(self.bins):
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202 acc += (i * val)
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203 count += val
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204
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205 if count > 0:
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206 return float(acc) / count
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207
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208 def max(self):
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209 return len(self.bins) - 1
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210
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211
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212 def memoize(func):
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213 if 'TESTING' in os.environ or 'DEBUG' in os.environ:
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214 return func
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215
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216 __cache = {}
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217 def inner(*args, **kwargs):
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218 k = (args, tuple(kwargs.iteritems()))
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219 if k not in __cache:
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220 __cache[k] = func(*args, **kwargs)
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221 return __cache[k]
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222
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223 inner.__doc__ = '(@memoized %s)\n%s' % (func.__name__, func.__doc__)
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224 return inner
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225
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226
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227 def quoted_split(s, delim, quote_char='"'):
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228 tokens = []
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229
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230 buf = ""
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231 inquote = False
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232
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233 for c in s:
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234 if inquote:
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235 buf += c
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236 if c == quote_char:
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237 inquote = False
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238 elif c == delim:
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239 tokens.append(buf)
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240 buf = ""
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241 else:
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242 buf += c
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243 if c == quote_char:
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244 inquote = True
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245
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246 if buf:
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247 tokens.append(buf)
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248
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249 return tokens