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view test-data/illuminapairedend.output.fastq @ 4:08d873cdd5f6 draft default tip
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/obitools commit dabf62d438facc62f3e606ff4419092fdcdfaa44
author | iuc |
---|---|
date | Wed, 20 Mar 2024 13:17:36 +0000 |
parents | f4e449660000 |
children |
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@HELIUM_000100422_612GNAAXX:7:119:14871:19157#0/2_CONS ali_length=62; direction=left; seq_ab_match=47; sminR=40.0; score=115.761290673; seq_a_mismatch=1; seq_b_deletion=1; seq_b_mismatch=13; seq_a_deletion=0; score_norm=1.86711759151; seq_b_insertion=1; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttgagctattgccggtagtactctggcgaatgattttgttataataattacttgtgtttagggctaagcatagtggggtatctaaaggaggcgg + dddddddddddbbdaddcddddddcadaddddaadcccWaaddabdx~~|b~~~~cccccccBcBcccBcBcccBccBBcc~}`kXyxu|~Kyr`b~~~~~~b~~~b~d\cdddddddddddddcacddddcdddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:108:5640:3823#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; score=231.881364714; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=2; seq_a_deletion=0; score_norm=3.74002201152; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaacaattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaaaggaggcgg + ddddddddddddddddbdddddbddddddddddddddddddddddd~~~~~~~~~~~~~~~~~~}~~~~~~~~~~~~~~~~~~~~~~~~~~~Z~~~~~~~~~~~~~b~d]dddddddcdddddcddddddddddddddddddddddcddddddd @HELIUM_000100422_612GNAAXX:7:97:14311:19299#0/2_CONS ali_length=62; direction=left; seq_ab_match=52; sminR=40.0; score=167.91734192; seq_a_mismatch=5; seq_b_deletion=0; seq_b_mismatch=5; seq_a_deletion=0; score_norm=2.70834422451; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctcttgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaaaggaggcgg + ddadddddcd`dcddddddddd^dd]ddddddZad`\`]Lcca``YT|~~^||v|z|~ts~~~|r~sZ^~~`~~~~~~~~~~~~~~~~V~b~]~~~~b~~~~~~~~`~b\aadddd`dcdddddc`dddccddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:22:8540:14708#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; score=223.982011431; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=3; seq_a_deletion=0; score_norm=3.61261308759; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgcctcctttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctaaggaggcgg + dddedddddddddedddddddddedddaddbddddddddddddedd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~b~~~~~~~~b~~~~ddddddddddabbb^bdddddddddddcddddddddcddddddddd @HELIUM_000100422_612GNAAXX:7:57:18459:16145#0/2_CONS ali_length=63; direction=left; seq_ab_match=60; sminR=40.0; score=227.883189945; seq_a_mismatch=3; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.61719349119; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg + ddcdddddddddddddddeddddddddddddddddddddcddddd~b~~~~~~b~~~~~~~~~~~~~~c~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddddddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:89:10281:5930#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; score=231.893859399; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.7402235387; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctgttgccgctagtactctggtgaacaattttgtttgtgtaattatttgtgtttagggctaagcatagtggggtatctaaaggaggcgg + ddddddddddddddddddcddddddddddddd\ddeddecddddddb~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddcddddddcdddddddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:29:15520:18035#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; score=239.965360923; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.87040904714; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgcctcctttagataccccactatgcttagccctaaacacaagtaattattataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctaaggaggcgg + cccccccc_cc_cccacacb]Y]_`a^__`_Y_`_a_`cc_ccccc~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~~~dddddddddddddddddddddddddddadddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:6:9274:14951#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; score=223.791171196; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=2; seq_a_deletion=0; score_norm=3.60953501929; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg + dcdddddddddddedddddddddcddddddcddddddddddddddd~~~~~~~~~~~~~~~~~~|z~}a|~~~~}}}}~~~~~~~~~~}~}b~b~~~~~~~~~~~~~~ddddddcdcddcd^ddddddddddddddddddddddeddddddddd @HELIUM_000100422_612GNAAXX:7:111:2893:6772#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; score=239.959941063; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.87032163005; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgcctcctttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctaaggaggcgg + dddddddddddddddddddddddddddddddddddedddddded_d~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~x~dddddddcdddcdcdddadddddddddddedddddddddddddddd @HELIUM_000100422_612GNAAXX:7:89:10621:20317#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; score=223.827654468; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=3; seq_a_deletion=0; score_norm=3.61012345916; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgcctcctttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctaaggaggcgg + ddddddddddddddbdddddadddddddd`ddddadadddddddRc~}~~z~~~~~~~t~sw}~~g}~~~~~~~~~~~~~~~~~~~~~~~~~~~~^~b`~~~~~~~~~ddddddddbdbdd_dddadddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:50:2791:11138#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; score=231.976017167; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.74154866398; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgcctcctttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctaaggaggcgg + dddddcdddddcddddcd^dddddddddbdbdddddddc\ddddbd~~~~~~~~~~~~~~~~~~b~~~^~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddbdddddddddcddddddddcddddddddddddd @HELIUM_000100422_612GNAAXX:7:108:17701:18887#0/2_CONS ali_length=62; direction=left; seq_ab_match=58; sminR=40.0; score=215.828321765; seq_a_mismatch=4; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.48110196396; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg + dd[dddddddd``ddddddddddddeddddddcdddddd\ddddddb~~~b~~~~~~~~~~~b~~~~~b~~~{~~{z|{|~~~~~~~~~~~ssx~~~~~~~~~~~~~~c^cddddddddddd`cedddddddddddddddddddddddddddcd @HELIUM_000100422_612GNAAXX:7:97:10414:9929#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; score=239.864541102; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.86878292101; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg + ddddddddddddddddddddddddddddddddcddddddcdddddd~~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddeddddddddddddddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:97:9091:15775#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; score=223.861916171; seq_a_mismatch=3; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.61067606728; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg + ddddddddddddddddddddddddddddddddcddddddddddddd~~~~b~~^~~~~~~b~~~~~~~|~~~~~~~~~x~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddddadcdddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:74:8683:20738#0/2_CONS ali_length=63; direction=left; seq_ab_match=62; sminR=40.0; score=243.961428864; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.87240363276; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; tcgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg + ddddddddddddddddddddddddddddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~`~~~~~~~~~~~~~~ddedddddddddddddddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:24:16230:12703#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; score=223.86908915; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.61079176049; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgaagtagtagaacaggctcctctagaaggggataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg + ddd`ddddad_ddaddddddddaddddddc`bO``ddd_a`ddd`a}}~sb{~~~~~~~~~~~~~~~~a~~~~~~y~u~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~b^ddcdbcddadddd_ddbdcdcdddd`ddddbddddddddddddd @HELIUM_000100422_612GNAAXX:7:4:4214:9434#0/2_CONS ali_length=62; direction=left; seq_ab_match=58; sminR=40.0; score=215.812703412; seq_a_mismatch=3; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.48085005502; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactacaggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg + ddddddddSddddbbdddddddbddabdddbdddddcddddddddd~^~~~{~{~~~~~}~~{~~ym~L||~~wuy~zyxPzjyyvxku~~~~~~~~~~~~~~~~~xbacdddddaddac_^`_`dcddddacddcddddadaa`[``^c`acc @HELIUM_000100422_612GNAAXX:7:12:17693:3451#0/2_CONS ali_length=62; direction=left; seq_ab_match=62; sminR=40.0; score=247.862243; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.9977781129; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg + dddddddddddddddddddddddddbddddddaddddddcdddddc~~~~~~~~~~~~~~~~~~~~~~~~~}~{~}{v}~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddeddddddddddcdeddddddcddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:3:14624:12375#0/2_CONS ali_length=63; direction=left; seq_ab_match=61; sminR=40.0; score=235.922125108; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.74479563663; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg + ddddddddddddddddddcdcddddcddcdcdddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~Y`~~~~~~~~~~~~~~~~~~~~~~~dddddcdddddddcedddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:18:5317:11571#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; score=239.862078449; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.86874320079; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg + ddddddddddddddddddddddddddddddddadddddddcddddd~~~~b~~~~~~~~~~{~~~~~~~~~~~~~~~~~y~~~~~~~~~~~~~~~~~~~~~~~~~~~~edddeddddddddddddddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:109:17335:11429#0/2_CONS ali_length=63; direction=left; seq_ab_match=56; sminR=40.0; score=195.716358124; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=6; seq_a_deletion=0; score_norm=3.10660885911; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg + ddddcdddddcdddddda`ddd^dd`^^`bdd`dR\]``]cdddd~x}~~W}}~~}~~~~~x~s~~z~ccccccccccccccccc~[|~`\~}``~|~~~~~~~~~b~dbdcddddddddadcdddcdcdddcddddaddddddddddddddd @HELIUM_000100422_612GNAAXX:7:7:10646:8487#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; score=231.85443709; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.739587695; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg + dddddddddddddddddddddddddddcdddddddddddddddddd~~~~~~~~~~~~~~~~b~~~~~~~hpoppitzv{~~~~~~~~~~~~~~~~~~~~~~~~~~~cedddddddddddddddccdddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:19:4865:20715#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; score=231.923942025; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.74070874234; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg + ddddddddddddddddddddddddddddcdddaddcddddddddddby~~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~bddddddddddddddbcdddddeddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:13:6954:13039#0/2_CONS ali_length=63; direction=left; seq_ab_match=62; sminR=40.0; score=243.872474161; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.87099165336; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatctagaacaggctcctctagagggatataaagcaccgccaagtcctttgagttttaagctattgctagtagtcctctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg + dddddddddddddddddddddddddddddddcddddddddddddd~~~~~b~~~~~~~~~~~~~~~~~~~~~~~r~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddcdddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:120:14963:1929#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; score=223.810433242; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.60984569745; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg + ddddddddddddddddddddddddddddddddcddddddddddddd~~~~_~~~~~~~~~~~~~~}~~~}}`z}~~~}~~}~~|~~u~~~~~~~~~~~~~~~~}~~b~dbdddddedddddddadddcddddcddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:115:3921:10649#0/2_CONS ali_length=63; direction=left; seq_ab_match=61; sminR=40.0; score=235.871015095; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.74398436659; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg + dddddddddddddddddddddddeddddddddddddddddddddd~b~~~~~~~~~~~~~~~~~~~~~~i~{n~}~}~~~~~~~~~~~~~~~~~b~~~~~~~~~~~~~dddddcdddddddddeddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:109:12848:8882#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; score=231.869965661; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.73983815582; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg + ddeddcddddddcdddddddddddddddddddTdddddddddddcdb~~~~~~~~~~~~~~~~~~~~~~}~~~b~x~~{~~~~~~~~~~~~~~~~~~~~~~~~~~~~~d`dcdddddddddddeeddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:84:11257:18886#0/2_CONS ali_length=63; direction=left; seq_ab_match=55; sminR=40.0; score=187.890540948; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=8; seq_a_deletion=0; score_norm=2.98238953885; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg + dddddddddddddddddddddddddddcddddddddddddddddd~t~~x~~~~~~~~~~~~~~~~~~~~t~~~~~~y~s}\`~~a|~b{~~~~~~b~~~~b~~a~b~ddddddddddddddcdddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:72:13060:5051#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; score=239.985823237; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.87073908447; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgcctcctttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctaaggaggcgg + ddbddddddddcbbdddcdcdddbd`cddddddddddddddddd_b~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddedddddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:35:3798:1896#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; score=239.863223783; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.86876167392; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgaagtagttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctactacttcgg + dddddddddddddbddddddddddddddddddddcddbddddddad~b~~}~~~~~~~~~~~~~~~~~n~~~~~|~}~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddcddaddaddd^dddbcddddddddadddddddddcdddddddd @HELIUM_000100422_612GNAAXX:7:117:12470:18434#0/2_CONS ali_length=63; direction=left; seq_ab_match=59; sminR=40.0; score=219.83660627; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=4; seq_a_deletion=0; score_norm=3.4894699408; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg + ddddddcddddddbdddcdddddbbdddddddddddcddddddcd~~~~~~~~~~~~~~~~~~~~~~~~~~~~O~~~~~~~~~{~~{~~Wx~~~~~~~~~~~b~~~b}ddabdddddddddddddddddddddddddddddddeddddddbdd @HELIUM_000100422_612GNAAXX:7:11:8428:13716#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; score=239.97387484; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.87054636839; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgaagtagttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctactacttcgg + ddddddddddddddddddddddddddddddddddddddddddddad~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddddddddddddddddcddddeddddddddddddd @HELIUM_000100422_612GNAAXX:7:78:18994:16521#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; score=239.927346216; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.86979590672; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg + ddcdddddcdddcdddcdddddddddcbdddd`dedddaeeddaddb~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dadddddddddcddddddcddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:16:5047:7843#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; score=251.979285107; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99967119217; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg + ddddddddddddddddddddddddddddedddbdddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddcdcddddddeeccdddddddcedddddeddddddddddd @HELIUM_000100422_612GNAAXX:7:118:13803:15594#0/2_CONS ali_length=63; direction=left; seq_ab_match=60; sminR=40.0; score=227.87720055; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.61709842144; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg + ddddddddddddddddddddddddddddddddddddddddddddd~~~~~b~~b~~~~~~~~~~v~~~~~~~~~~y~{~~~~~~~~~~~a~~~~~~~~~~~~~~~~~~dddedddddbdddd^ddeddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:53:14379:5922#0/2_CONS ali_length=62; direction=left; seq_ab_match=62; sminR=40.0; score=247.982071703; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99971083392; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgcctcctttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctaaggaggcgg + dddcdddddddddddddddddddddddddddddddddddddddd`d~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddeddddddddddddddddddcddddddddddddd @HELIUM_000100422_612GNAAXX:7:52:10700:16508#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; score=239.790995416; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.86759670025; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg + dddddddddddddeddddcdddcdddddddddIddbdddbdcddddb~~u~~~}~|||v|~~~~{~~~~~}}}~~~~~~~~~~~~~~~~v~~{{~~~~~~~~~~~~~~ccccccacccccccccc^cccccccccccccccc_cccccc[cca^ @HELIUM_000100422_612GNAAXX:7:69:7696:12881#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; score=251.983052061; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99973098509; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg + dddddddddddddddddddddddddddeddddddcdddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddcdddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:44:12484:5320#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; score=231.837799589; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.73931934821; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg + ddddcbddddddccddddeddddddddddddddddddddaddddcda~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~z}}}~~~~~~~~~~~~~~~~~~~cddddddddddddddddeddddddddddddddddddddddeddddd @HELIUM_000100422_612GNAAXX:7:21:4713:10229#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; score=239.864510201; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.86878242259; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg + dddddddddddddddddddddddddddbdddd`ddddddddddddd~~~~~~~~~~~~~~b~~~~~~~~~~~~~~~~~~~|~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddcddddddddbdcdddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:38:6201:12003#0/2_CONS ali_length=63; direction=left; seq_ab_match=59; sminR=40.0; score=219.686655506; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=4; seq_a_deletion=0; score_norm=3.48708976993; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatctagaacaggctcctctagcgggatataaagcaccgcccagttctttgagttttaagctattgctagtagtcctctggcgaattattttgttgtaaaattatctatgtttagggctaagcatagtggggtatctaagatattcgg + bbb`^a``b[a[aa^YVY\YW__TXbbHaDDDDDDDDDDDDDDDDcBcccBccccccccccccccccc~x~z}}}}}y~rzwzz||{zyzxa{}~t~~~~~~~b~~~~cddddd[dddcddcddbddbdddddd`dddd^dcddddddddddd @HELIUM_000100422_612GNAAXX:7:33:16506:1490#0/2_CONS ali_length=62; direction=left; seq_ab_match=62; sminR=40.0; score=247.963002972; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99940327375; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg + ddddeddddddddddddddddddddddddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddeddddddddddddddddddddddddddeedddd @HELIUM_000100422_612GNAAXX:7:1:9007:3289#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; score=231.83380127; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.7392548592; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccatctctctagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaagagagatgg + dddddddddddddbddddddddddddddddddddddddddddddddb~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~}~~~~~~~~~~~~~~~~~~~dbddcddddddddddddddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:32:4360:3451#0/2_CONS ali_length=61; direction=left; seq_ab_match=61; sminR=40.0; score=243.999335649; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.999989109; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=47; seq_b_single=47; cccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg + dddeddcddddddcdddddddddddddddddddddddddddddddde~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddeddddddddddeeddddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:115:17402:4174#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; score=223.877240886; seq_a_mismatch=3; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.6109232401; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgcaggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaaaggaggcgg + ddddddddddddddddcddddd`addddddddcdddddddddddddb~~~b~~~~~~~~~~~~~~{~~~~~~Sv~y|~s~~~~~~~~~~~~~~~~~~~~~~~~~~~~~d]ddcdeddddccdddddeddddcdddddddddddddddddddded @HELIUM_000100422_612GNAAXX:7:45:6831:6228#0/2_CONS ali_length=62; direction=left; seq_ab_match=62; sminR=40.0; score=247.974637619; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99959092934; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg + ddddddddddddddddddddddddddddddcddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddedddddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:117:4291:18111#0/2_CONS ali_length=63; direction=left; seq_ab_match=62; sminR=40.0; score=243.911625561; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.87161310415; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg + dddddddddddddddddddddddddddddddddddaddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~ddddddddddddddddddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:71:14800:3222#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; score=231.89389474; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.74022410871; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaaaggaggcgg + ddddddddddddddddddeddddddddeddddcdddddccdddddd~~~~b~~~~~~~~~~~~~~~~~\~~~}~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dcddddddddeedddddddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:11:8547:18238#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; score=251.984413754; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99975259927; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg + ddddddddddddddddddddddddddddbdcdddddddddcdddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddddddddddddddddddddeddddddddddddd @HELIUM_000100422_612GNAAXX:7:117:2416:10342#0/2_CONS ali_length=63; direction=left; seq_ab_match=61; sminR=40.0; score=235.921215944; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.74478120546; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg + dddddddddddddddcdcddddaddcddddddddbddddbddddd~b~~|b~~~~~}~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddcdcddddY[ZYUd^dbdbdddddddddddddcdddddddddd @HELIUM_000100422_612GNAAXX:7:78:7151:19928#0/2_CONS ali_length=63; direction=left; seq_ab_match=56; sminR=40.0; score=195.576462373; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=7; seq_a_deletion=0; score_norm=3.10438829164; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacgagaatgttcgccagtgtacttctagcaacaggctgaaactcaaaggacttggcggtggtttacatccctctagaggagcctgttctagatattcgg + ZYQOYVVXRYYOYZZ^^^_bZZ]Z[S[WSR]YbbbDDDDDDDDDDcccccccccccBBcccccccccccccBcccccBccccccccccBccccccccccBccccc~`~WNS]Y_KTUVZ[O_SHW]ZQ_^^^ab`Obb\TY\MPPPPQQQQQQ @HELIUM_000100422_612GNAAXX:7:71:18821:20471#0/2_CONS ali_length=62; direction=left; seq_ab_match=53; sminR=40.0; score=175.889514148; seq_a_mismatch=7; seq_b_deletion=0; seq_b_mismatch=2; seq_a_deletion=0; score_norm=2.83692764755; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg + ddddddddddddddddddddddddddddd\ddddddddd^ddd`dd~~~~Xx~~~~~{|q}}x}^Y`J`~Xv{wt~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~sbddddddddddddd^bdccddbdddddd`ddddddddddddddcddd @HELIUM_000100422_612GNAAXX:7:58:15837:8531#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; score=251.96128051; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99938540492; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg + ddddddddddddddddddddddddddddddbdddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddcdddddddddddddcdddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:38:10813:16867#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; score=251.97777927; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99964729; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgtttgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg + dddddddddddddddddddddddcdddddddddddeddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dbcdddddadadcdddddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:68:19115:18847#0/2_CONS ali_length=62; direction=left; seq_ab_match=54; sminR=40.0; score=183.856571062; seq_a_mismatch=6; seq_b_deletion=0; seq_b_mismatch=2; seq_a_deletion=0; score_norm=2.96542856552; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaaaggaggcgg + cdbdddddddcdbcddc[Tabcddd_`bddaaabd^bb^P`ccd`db{~~b~~~~~~\\~~x~~~~s~~s~~~_~~{~~~~~~~b{~~}~~~~~~~~~~~b~~~~~`~ddd^dd_ddcdd_ddceddedcdddddcddacdddddddddddcad @HELIUM_000100422_612GNAAXX:7:14:3978:11028#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; score=231.869517243; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.73983092328; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgcaggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg + ddcddddddcdddeddddddddcddddddcb`Zbbddddddddddd_~~~~~~~~~~~~~~~~~~~v~~~y~J|~u{|w~~~~~~~~~~~~~~~~~~~~~~~~~~~~~da`dddeddddcdbdaeddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:34:18733:3827#0/2_CONS ali_length=63; direction=left; seq_ab_match=62; sminR=40.0; score=243.983236898; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.87274979204; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg + ddddddddddddddddddddddddddddddddddddddddddddd~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddddddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:65:6787:8456#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; score=231.869006954; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.7398226928; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg + ddddddddbddddeddddedddddddddddddddddddddddddddb~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~d_ddeddddddcdddddddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:111:7441:14688#0/2_CONS ali_length=63; direction=left; seq_ab_match=62; sminR=40.0; score=243.832560428; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.87035810203; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg + ddddddddddddddddddddddddddddddddddddddddddddd~~~~~b~~~~~u~~~~~~~~~~~~~~~~~~}~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddedddddddddadddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:86:10092:15563#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; score=223.927744132; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.61173780858; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgcctcctttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctaaggaggcgg + dddd`dddddbdddddddddddcddddddbdddddccdddddaddd~b~~~~~~~~~~~~~_~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~|bddddddddddcddcddd`^dddddddddddcddddddddddddbdd @HELIUM_000100422_612GNAAXX:7:16:4418:5128#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; score=223.862543287; seq_a_mismatch=3; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.61068618206; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg + dddddddddddddddddddddddddddddddddddddddbddedddb~~~b~~b~~~~~~~~~~~~~~~{~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~bddddddddddddddedddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:13:5742:9859#0/2_CONS ali_length=58; direction=right; seq_ab_match=55; sminR=40.0; score=207.846895043; seq_a_mismatch=3; seq_b_deletion=50; seq_b_mismatch=0; seq_a_deletion=50; score_norm=3.58356715591; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=0; seq_b_single=0; ccgcctcctttagataccccactatgcttttctagaggagcctgttctaaggaggcgg + ~~~~~~Z~~~~~~~~~b~~~~~~~~~_~~~~~~~~~~}~~~~~~~~~~~~~~~~~~~~ @HELIUM_000100422_612GNAAXX:7:85:18986:17662#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; score=231.874929984; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=2; seq_a_deletion=0; score_norm=3.73991822554; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgaagtagttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctactacttcgg + ddcdddddddddeccdddddddddcbdddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~}~~s~~~~~b~~~~~~b~~~~~~~ddcddddddcddddddddddddd`dddddddddddddddddddded @HELIUM_000100422_612GNAAXX:7:16:16357:19948#0/2_CONS ali_length=63; direction=left; seq_ab_match=57; sminR=40.0; score=203.860719115; seq_a_mismatch=5; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.23588443039; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcacaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg + baNdQdd`dd`da_ddbadbdddcddddd_dbddbdaddbddddd~`~~b~~~~~~b~~{~~~}x}}~}`twyhw~~~~}w|~|~~~~~X~~~|~~~~~Y~~~~~~~~XVRSQ[Qadd``^P^adYdddddddcc`^_dccddcdccdddddd @HELIUM_000100422_612GNAAXX:7:107:3570:18147#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; score=231.924768153; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.74072206698; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttatgctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaaaggaggcgg + ddada`dcddddddddddddddadd_^b_cdd]dddddcdd_dcdd`~s~a~~~~~~~~}~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~d\d\dddddcdddddadddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:82:8836:11354#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; score=231.781218203; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.73840674522; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg + dddddddddddddddddddddddddddaddddcdddddcaddddddb~~~b~~~~~~~~~~~~~~~~~~~~~|~~k{{w~~~~~~~~~~~~~~~~~~~~~~{~~~~~~ddddddddddddddddeddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:41:2354:1449#0/2_CONS ali_length=63; direction=left; seq_ab_match=55; sminR=40.0; score=187.777927438; seq_a_mismatch=5; seq_b_deletion=0; seq_b_mismatch=3; seq_a_deletion=0; score_norm=2.98060202283; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg + ddbdddccddddddddddddddddddddaaddddddcddbcdddd~b}bb~b~~~b~q~~}~}~x~x~t~s~|k|~w~~z~~{|{~ccBc~~}~~~~~~a~~a~}p~~baceddbbedddda_aa_c[cdadddddddddddddddadddadd @HELIUM_000100422_612GNAAXX:7:114:4511:17596#0/2_CONS ali_length=62; direction=left; seq_ab_match=57; sminR=40.0; score=207.883730153; seq_a_mismatch=4; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.35296338956; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgcctccttagaacaggctcctctagaagcgtataaagcaccgccaagtcctttgagttttaagctattgccggtagcactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaaaggaggcgg + ddeedecddddddbdddcdeeddddPQPPPKKPQPPPPPQdddddd~~~~b~~~~~~~~~~~~~~~~~as~a~~~~~}W~~~~~~~~~~~~~X~~~~~~~~~~~~~~~d^d^dddddddddddddddddddddddbdddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:1:8849:9880#0/2_PairEnd sminR=40.0; pairend_limit=108; ali_dir=left; score=25.9064763088; mode=joined; sminL=40.0; ggacgggagagggggggggggggggaaggggggggttttggggggggggggccgttttaaaaaaaaaaaaaaaacaacgtgacatggcacagccgtcacaactgtcagctgacgaccactggcagtgagtctactctcctattttgttttttttttcaagcagaagacggcatacgatatcggtctcggcattcctgctgaaccgctcttccgatc + BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB_`X`b[`T[ZWHZTWU]]b]]TZ^__\^`\OOOOO^`^Z^_ba_bbbbbb @HELIUM_000100422_612GNAAXX:7:94:12367:17729#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; score=223.888596169; seq_a_mismatch=3; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.61110638983; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg + dddddddddddddddddddddddddddcceddccdadbd`dddddcb~~~b}~~~~~~~~~~~~~~~~~x||t~|r~~~~~~~~b~~~~~~~~~~~~~~~~~~~~~~~daddedddddddddd`dddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:30:5374:18213#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; score=251.958026467; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99933375344; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg + dddddddddddddeddddddddddddddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddddddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:49:11740:5547#0/2_CONS ali_length=63; direction=left; seq_ab_match=62; sminR=40.0; score=243.959827817; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.87237821931; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg + ddaddcdddddddedddddddddddddeddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~cddddddddcdddddddddddddddddddddedddddddddd`dd @HELIUM_000100422_612GNAAXX:7:29:3531:8314#0/2_CONS ali_length=63; direction=left; seq_ab_match=61; sminR=40.0; score=235.837856208; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.74345803504; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg + dddddddddddddcddddddddddddddddddcddddddddddcd~a~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~}~~~~~~~~~~~~~~~~~~~~~ddceddddddddddddddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:114:10615:11217#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; score=231.702876359; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.73714316708; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgcctcctttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctaaggaggcgg + ddcdddcddcd`d__^dddddd`cddddddddaaa``a_adddd^d~b~~z~~~~~~~~~~~~~cBcccccccccccccc~y~~s~~~~~~~|~~~~~|~~~~sz~~~adddddd[dad`VRW]VZdddddddddddddc``_ccddddddddd @HELIUM_000100422_612GNAAXX:7:108:2729:8600#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; score=223.856778095; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.61059319508; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg + dddddddddddddeddddddddcddddddddddWddddcddddddd~~~~b~~~~~}~~~~~~~~~~~`~~~~~~~~~~~~~~~~~~~~~~~a~~~~~~~~~~~~~~~dacdcdeddddddddddddddedddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:103:8847:6326#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; score=251.968264217; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99949625741; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg + ddddddddddddddddddddddddddddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddcddddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:70:11798:2668#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; score=239.988667111; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.8707849534; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgaagtagttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacctttctagaggagcctgttctactacttcgg + dddddddddddddddddddddddddddddddddddddddddddddd~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dcdddddcddddddddbddedddddddcdeddeddddddddddddd @HELIUM_000100422_612GNAAXX:7:4:18261:9148#0/2_CONS ali_length=63; direction=left; seq_ab_match=61; sminR=40.0; score=235.937993338; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.7450475133; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg + ddeddddddddddcdddddddddddddeddddddddddddddddd~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~`~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddddddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:29:18416:16620#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; score=231.873033258; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.7398876332; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgcctccttagaacaggctcctctagcagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagaactctggcgaataattttgttataataattacttgtgtttagggctaagcatagtggggtatctaaaggaggcgg + ddddddddddddddddccddddddd`d\dd`bH[`bbb^bdddddd~~~~~~~~~~~~~~~~~~~~~~~~~`~s~u||Fz~~~~~~~~~~~~~~~~~~~~~~~~~~~~d`ddcdbdcddbbbb\dadddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:32:18262:6966#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; score=239.975704028; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.87057587142; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg + edddddddddddddddddddddddddddddddddcddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~`~~~~~~~~~~~~ddddddddddbdddddddddddddcdddddddbddcdcddcdddbb @HELIUM_000100422_612GNAAXX:7:110:17877:11976#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; score=223.833250149; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.61021371209; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaaaggaggcgg + ddddddddddddddddddddddddddd_ddddadddddddddddddb~~~b~~~~~~~~~~~~~~~~~~~vqx{w~~~~~~~~~~x~~~~~~~~~~~~~~~~~~~~`~b\d^dddddddddddcdddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:97:9416:9155#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; score=231.872797448; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.7398838298; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg + ddddddddddddddddddddddddddddddddeddddddddddaddb~~~~~~~~~~~~~~~b~~~~~~{~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~eddddddddddddddedddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:72:8905:10715#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; score=251.929473647; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99888053408; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg + dddddddddddddeddddedddddddddddcdddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddcdddddadddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:48:5296:16375#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; score=251.994265764; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99990898038; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg + dddddddddddddcdddd`ddddddddcddbdddddaddddbddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddd`dbdaaddddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:53:6550:1163#0/2_CONS ali_length=63; direction=left; seq_ab_match=61; sminR=40.0; score=235.996766828; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.74598042584; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgtttgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg + dddddddddddddddddddddddedddddaddddddddddddddd~b~~~~~~~~~~~~~~~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddddddddddddddddddddeddddddddddddd @HELIUM_000100422_612GNAAXX:7:120:1900:13295#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; score=219.874676069; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=1; score_norm=3.54636574304; seq_b_insertion=0; seq_a_insertion=1; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgcgcgtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg + dddddddddddddcdddddddddddddddddddcdddddcdddddd~~~~b~~~~~~~~~~~~~~~~~~~opfFhtpp~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dcddddcdddcdddddcdddddddddddddddeddddedddddddd @HELIUM_000100422_612GNAAXX:7:96:19317:7551#0/2_CONS ali_length=62; direction=left; seq_ab_match=58; sminR=40.0; score=215.85731879; seq_a_mismatch=3; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.48156965791; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg + ccccccbccccccccccccccc^cccccccbcXcbccccccccccc~q~|a~~~~~~|~~~~az~~~|_snppphy~~xq{tzz~~~~~~~~Z~~~~~~~~~~~~~~~d_ddddddddcddd`bbdddddddcddddddddddddddddddddc @HELIUM_000100422_612GNAAXX:7:93:18556:10479#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; score=223.913795643; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.61151283295; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgcctcctttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctaaggaggcgg + ddddddddcddcddcddddddddddddddddddddddddddddddd~b~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~~~dddddddddddddbdddddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:44:19605:15509#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; score=223.916231984; seq_a_mismatch=3; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.61155212878; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaaaggaggcgg + dddddddddddddddddddddddddbddddddcddcdcdacddcdd~r~~`~k`~~p~~~_~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~z}y~~~~~~~~~~~~~~a_d_dddddddddddcdadddddddcdddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:40:3610:8896#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; score=239.967241748; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.87043938304; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgaagtagttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctactacttcgg + dddddddddddddddddddddddddddddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~~~~~dddddddddddddedddddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:41:19763:3556#0/2_CONS ali_length=62; direction=left; seq_ab_match=62; sminR=40.0; score=247.825851895; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99719115959; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg + ddddddddddddddddddddddddddddddddcddddddddddddd{~~~~~~~~~~~~~~~~~~~v~~~~~~~~jz{m{~~~~~~~~~~~~~~~~~~~~~~~~~~~~dacddddddddddddcddddddddddddddededddddddddddWd @HELIUM_000100422_612GNAAXX:7:111:7660:7465#0/2_CONS ali_length=63; direction=left; seq_ab_match=58; sminR=40.0; score=211.768626837; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=3; seq_a_deletion=0; score_norm=3.36140677518; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg + dbdbddcdddddddddd^dd`dddddcddcdddddd`ddddddcd~~~~~~~~_~~~~~~~~~~~~~Ky~~~~~~c~Y}~~s~~`~x~}~}~{~Z|h~~~~~~~~~~~dYYdddaddaaddad^dddcddddddcdddddddadcdddd``dd @HELIUM_000100422_612GNAAXX:7:76:10822:18722#0/2_CONS ali_length=63; direction=left; seq_ab_match=58; sminR=40.0; score=211.83390837; seq_a_mismatch=5; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.36244299; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacacgaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg + ddddddbcdbdddddddd_dddddddcacbdcddddTbdcddddd~`~~~z~~~~~~S~~~~~~b~~`~~~t~t~~}~~~~~~~~~~~T~~~|~~~~~~~~~~~~x~~ddddadddd_ddbdcbdddadddcdddddddbdddddddbddddd @HELIUM_000100422_612GNAAXX:7:76:8491:8900#0/2_CONS ali_length=62; direction=left; seq_ab_match=57; sminR=40.0; score=207.828655885; seq_a_mismatch=4; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.35207509492; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgcaggtagcactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg + dcddddddddddddadddddddadddcaddddadcdddddddddddb~~~b~~~~~o~~~~~~~~~~~~~z}F}~xq~N}~~y~~~}~~~~wu~~~~~~~~~~~~~^~b[bcc_ccccbcccccccbcbabacccccccbc_ccbbb`bccccc @HELIUM_000100422_612GNAAXX:7:47:7619:9866#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; score=239.867362445; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.86882842653; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg + dddddeddddddedddddddddeddddddddddddddddddddddda~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dedddddddddddddddddddddddddddddddddddddddedddd @HELIUM_000100422_612GNAAXX:7:1:8649:5297#0/2_CONS ali_length=104; direction=right; seq_ab_match=104; sminR=40.0; score=415.823014771; seq_a_mismatch=0; seq_b_deletion=4; seq_b_mismatch=0; seq_a_deletion=4; score_norm=3.99829821895; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=0; seq_b_single=0; ccagcgagatcccattgagtctctgcacctatcctttttttcgttttctgaatctttgtttggaaaaaaacaggatttggctcaggattgcccgatctcgctgg + ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @HELIUM_000100422_612GNAAXX:7:99:14742:5591#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; score=231.982488051; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.74165303308; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgcctcctttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctaaggaggcgg + dddddddddddddddddeddddddddddddddddddddddddddcd~b~~~~~~~~~~~~~a~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddddcddddddddddcdddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:95:3274:5435#0/2_CONS ali_length=63; direction=left; seq_ab_match=61; sminR=40.0; score=235.944669323; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.74515348131; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg + dddddddddddcddddddddddccddddddddddcdddddddddd~~~~~b~~~~~~~~~~~~~~~~~~~~~~~~~{~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~ddddcdddddddddcdddddddddddedddddddeddcdcddddd @HELIUM_000100422_612GNAAXX:7:74:8396:18135#0/2_CONS ali_length=63; direction=left; seq_ab_match=61; sminR=40.0; score=235.858013544; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.74377799276; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg + ddddddddddddddddddddddddddddddddddddddddddddd~W~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddcddddddddddddddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:9:10358:4089#0/2_CONS ali_length=61; direction=left; seq_ab_match=54; sminR=40.0; score=181.620394786; seq_a_mismatch=1; seq_b_deletion=1; seq_b_mismatch=6; seq_a_deletion=0; score_norm=2.97738352108; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=47; ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagttctttgagttttgagctattgcctgtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg + ddcddddddddbdddddddddd`ddadddddda`]][\[]cdd`ccacccBcccccccccccBccccccccccBccccBcccccccccccccccc~~~ZzzzwX~yz`^dcddcddddddabdad^dddbbcbdddddddbdbbcddddddddd @HELIUM_000100422_612GNAAXX:7:78:10338:12302#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; score=231.881878067; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.74003029141; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg + ddddcddddddddddddddddddddddddddddddddddddddddd~a~~~~~~~~~~~~~~~~~~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddeddddddddddddddddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:95:17941:6113#0/2_CONS ali_length=63; direction=left; seq_ab_match=57; sminR=40.0; score=203.869927289; seq_a_mismatch=6; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.23603059189; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg + ddedddddddddedddddddddddddddddddddddddddddddd~b~~~b~~b~~~bb~~~~~~~~~~~~~~~~~~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~acede^cdddc`dd`ddddedadddedddddddddddcdddaacc @HELIUM_000100422_612GNAAXX:7:59:11971:12063#0/2_CONS ali_length=62; direction=left; seq_ab_match=62; sminR=40.0; score=247.995051728; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99992018915; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgcctcctttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgtcagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctaaggaggcgg + ddddddddddddddddddddeddddddacddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~|~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddcdddddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:47:3878:6141#0/2_CONS ali_length=62; direction=left; seq_ab_match=62; sminR=40.0; score=247.947034203; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99914571295; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg + dddddddddddddddddddddddddddddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddddddddddddddddddddcddddddddddddd @HELIUM_000100422_612GNAAXX:7:21:5487:19582#0/2_CONS ali_length=62; direction=left; seq_ab_match=58; sminR=40.0; score=215.831528178; seq_a_mismatch=3; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.4811536803; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg + dddaddbddddcdcddddddddddddddbddcdddcddddddddd_~v}~~}{~~~~b~}ZZ}}}t~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~d`dbdddbdcdcdacddddddddddddaddddddddddeddddddd @HELIUM_000100422_612GNAAXX:7:38:13721:2004#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; score=239.856433937; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.86865216027; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg + ddcccd`dddddddddddddddddddbbddbd^ddabddddddddd~{~~~~~~~~~~~~b~~~~~~~~~~~~~~~}}~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~d`ddddddddddddddd]addddbddddddddcddddddddddddd @HELIUM_000100422_612GNAAXX:7:111:19168:18517#0/2_CONS ali_length=63; direction=left; seq_ab_match=60; sminR=40.0; score=223.825469474; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.55278522975; seq_b_insertion=0; seq_a_insertion=1; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=46; ccgaagtagttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattatccgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctactacttcgg + ddddddddddcddddddddddddddcdddbddddddbcdddd^dd~~~{~_~~~~~~~~~y{}{~U~{|~~~~~n~~|~~~~}x~~~~~~~~~~~~~~~~~^~~~~~~ccdaddbdddbddb_ddddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:89:16162:8889#0/2_CONS ali_length=63; direction=left; seq_ab_match=62; sminR=40.0; score=243.971622168; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.87256543123; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattg + dddddedddeddddddddddddddddddddddddddddddddddd~~c~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddedddddddeddddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:74:4888:6953#0/2_CONS ali_length=63; direction=left; seq_ab_match=61; sminR=40.0; score=235.986171514; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=2; seq_a_deletion=0; score_norm=3.74581224625; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg + ddddddddddddddddddddddddddddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~]~~~~~~~~~~~~~~~~~~~dddddddddeddddddddddddddddddddcdddddddddddddd @HELIUM_000100422_612GNAAXX:7:41:16707:19819#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; score=231.94731954; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.74108579903; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaaaggaggcgg + dedddddddddddbddedadddddccddddddaddbddd^a`daddb~~~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~bbdddddddddddddddddddddddddddddddccddddddddddd @HELIUM_000100422_612GNAAXX:7:7:7916:16505#0/2_CONS ali_length=58; direction=right; seq_ab_match=58; sminR=40.0; score=231.999536374; seq_a_mismatch=0; seq_b_deletion=50; seq_b_mismatch=0; seq_a_deletion=50; score_norm=3.99999200644; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=0; seq_b_single=0; ccgcctccttagaacaggctcctctagaaaagcatagtggggtatctaaaggaggcgg + ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @HELIUM_000100422_612GNAAXX:7:41:9316:2256#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; score=239.993848494; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.87086852409; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgcctcctttagataccccactatgcttagccctaaacacaagaaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctaaggaggcgg + dddddddddddeddddcdddddddddddddddddddddddddddNd~~~~~~~~~~~~~~~~~~~~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddcadedddddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:67:7582:3642#0/2_CONS ali_length=63; direction=left; seq_ab_match=62; sminR=40.0; score=243.962430842; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.87241953718; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg + ddddddddddddddddddddddddddddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~`~~~~~~~~ddddddddddddddddddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:42:8435:4155#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; score=251.987540492; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99980223003; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg + ddddddddeddddddddddddddddddddddddddddd`dddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddddddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:1:8702:15835#0/2_CONS ali_length=85; direction=left; seq_ab_match=85; sminR=40.0; score=339.959438284; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99952280334; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=23; seq_b_single=23; cccatcgctcagatatccgttgccgagagtcgtgtagattatatagaattgcaactcggggtgcggccagcaagccagccacatcccctcgttaggcacagtgttccttgacgccttcggtgagcgatggg + ddddddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~{~~~~~~~~ddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:33:9900:9541#0/2_CONS ali_length=62; direction=left; seq_ab_match=62; sminR=40.0; score=247.949713348; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99918892497; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccaattaacttagataccccactatgcctagccctaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg + dddddddddddddcddddddddddddddddddd\`cccccdddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddedddddddddecddcddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:48:7139:3789#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; score=251.902451987; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99845161885; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg + ddddddddddddddddddddddddddddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddddddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:46:13269:4134#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; score=251.997344433; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99995784814; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg + dddddddddddddeddddddddddddddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddcdddddddddddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:46:9246:19171#0/2_CONS ali_length=62; direction=left; seq_ab_match=52; sminR=40.0; score=167.802718427; seq_a_mismatch=10; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=2.70649545851; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgcctcctttagataccccactatgcttagccctaaacacaagtaattattataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctaaggaggcgg + ddadddddddcaddddddddbdcdddd`dddddddddd^bddadcd~b~~~~~b~~~~~~~_`~~~~~b~~~~~S~~~~~~~~~~b~~n~`~~k~zoY|~~~~jb~~~DDDDDDDDDDDDDDDDDD`M\^ca__T^^Y^c^c_[`[ddddd`cd @HELIUM_000100422_612GNAAXX:7:37:12979:6595#0/2_CONS ali_length=58; direction=right; seq_ab_match=58; sminR=40.0; score=231.998796685; seq_a_mismatch=0; seq_b_deletion=50; seq_b_mismatch=0; seq_a_deletion=50; score_norm=3.99997925319; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=0; seq_b_single=0; ccgcctcctttagataccccactatgcttttctagaggagcctgttctaaggaggcgg + ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @HELIUM_000100422_612GNAAXX:7:95:1688:9598#0/2_CONS ali_length=63; direction=left; seq_ab_match=54; sminR=40.0; score=179.847313607; seq_a_mismatch=6; seq_b_deletion=0; seq_b_mismatch=3; seq_a_deletion=0; score_norm=2.8547192636; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtatcactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg + aaaddbdbddbd`bdc^`[cddbddd`d_ddddcd^^`[`ddd[d~~~~pW~~`xzxwr~p{vvwxjwItwwrxxm|FPS~~}}}~z|~~__~~~~~~~~~~~~~[~~bbacb`adcdddd`cddddddddbbddddddddddddcdddcddd @HELIUM_000100422_612GNAAXX:7:55:16528:11418#0/2_CONS ali_length=62; direction=left; seq_ab_match=58; sminR=40.0; score=215.988419267; seq_a_mismatch=4; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.48368418172; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcactagcttacaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattag + ddddbadddddddccddccddddaaadddcdddddddddddcd`da~~~~~~~~~~~~x~~~~~\~~~~~~~~~v~~~~~~~~~~\~~~~~~~[~~~s~~~~~~~~]~cY__]\QPPPP`]`[`c^^[cccccccccccaba_a\``]_WK[T[ @HELIUM_000100422_612GNAAXX:7:32:18486:7018#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; score=251.909476689; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99856312205; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg + dddddedddddddddddddddddddddddddddddddddddedcd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddeddddddddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:85:2105:21152#0/2_CONS ali_length=63; direction=left; seq_ab_match=57; sminR=40.0; score=203.706262238; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=4; seq_a_deletion=0; score_norm=3.23343273394; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg + ddddddddddddddddddddddddccdddddcddedddeddddbd~b~~{~~~~~}~}\~~~~cccccccccccccccccc~~~x~j~~~~~~~~~b~`s~b~~~~b~d`dddaddddddddddaddaddddcdddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:83:6795:14061#0/2_CONS ali_length=62; direction=left; seq_ab_match=62; sminR=40.0; score=247.992382324; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99987713426; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgcctcctttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctaaggaggcgg + dddddddddddddcddddddcdcddddddddddddddddddddd_d~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddcddddddddddbdddbdddddddddcddddeddddddddddddd @HELIUM_000100422_612GNAAXX:7:73:16525:10764#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; score=231.90336822; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.74037690678; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaaaggaggcgg + ddcddddddddddedddddddddddddadddd[bddddb_dddddd~~~~~~~~~~~~~~b~b~~~~~|~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b^ddddcddcddddddd_cdddddcddbdddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:19:6313:16567#0/2_CONS ali_length=63; direction=left; seq_ab_match=60; sminR=40.0; score=227.826165596; seq_a_mismatch=3; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.61628834279; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagccctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg + ddbcddddcdddbdcdcdddddadddcddcdYab`__b_bddcdd~~~~O_~~~~~}~~u}~~~~~~~a|~~~z{mrwvx~r~|w}}~~~~~~~~~~~~~~~~~~~~~dddddcaddddddcddddddeeddddddddebddddddddddddd @HELIUM_000100422_612GNAAXX:7:114:8865:19510#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; score=239.913762365; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.86957681233; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg + ddddedddddddddddddddddcddddddddddddddddddddddd~a~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddcdddddddddddddddedddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:111:8478:1127#0/2_CONS ali_length=63; direction=left; seq_ab_match=57; sminR=40.0; score=203.794981474; seq_a_mismatch=5; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.23484097578; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgcgttccaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg + dddd_ddddcdadddddcddddbdddddcd^dabdddcaddddda~]v~~Z~~`puIwxxH~|~}~~~~~~~|~~y}vp~{~~~~|yz~~~~~~~{~~~~~~|~~~b~ddbMddddddddddadddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:81:15726:2345#0/2_CONS ali_length=63; direction=left; seq_ab_match=56; sminR=40.0; score=195.641193686; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=7; seq_a_deletion=0; score_norm=3.10541577279; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccgagtgctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg + ````Z]]cbb`ccbcc[`ca`bU[cQZ___cQbc`DDDDDDDDDDcBcccBccccccccccccccBccccccccccBcBccccccBccBc~~~~qp~wws~~{~~s~~b_cc__ba_accb_cXJWXWaabcac_c`[__[[_]_aa__`___ @HELIUM_000100422_612GNAAXX:7:13:14386:20412#0/2_CONS ali_length=63; direction=left; seq_ab_match=60; sminR=40.0; score=223.918527801; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.55426234604; seq_b_insertion=1; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=45; ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg + dddddddddddddddddddddddddddcdddddddddddddddddd~~~~b~~~~~~~~~~~~~~~~~~t~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ax~~~~a~~dddddddddddddddcedddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:83:18882:9967#0/2_CONS ali_length=63; direction=left; seq_ab_match=60; sminR=40.0; score=227.922890965; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.6178236661; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg + ddddddddddddddddddcdddddddddddddddddddddddddd~b~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~~~~~~~~~~dddddddddddddddddadddddddddcddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:13:17353:13234#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; score=223.801902424; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=2; seq_a_deletion=0; score_norm=3.60970810361; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgaagtagtagaacaggctcctctagaaaggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg + ddddedddddddddddddddddddcdddddbcUcbdddbddbddadb~~~~~~~~~~~~~~~~~~~~~~~~~~~~~|~~~~~~~~~~~~~]pVo~~~~~x~~~~~}~~b`dWddeddddddddddddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:53:10153:11845#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; score=239.985596162; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.87073542196; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgaagtagttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctactacttcgg + dddddddddddddcddddddddcddddddddddddddddddddddd~~~~~~~~~~~~~~~a~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddedddddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:33:9296:13526#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; score=239.852481933; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.86858841828; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg + ddddadddddddddddddddddcdddddcdddZdddddbddddddd^~~~~~~~~~~~~~~~~~~~~~~~v~~~~~|~|~~~~~~~~~~~~~~~~~~~~~~~~~~~~~d^ddddddddeedddddcdddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:113:2918:18736#0/2_CONS ali_length=62; direction=left; seq_ab_match=58; sminR=40.0; score=215.87931956; seq_a_mismatch=4; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.48192450903; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaaaggaggcgg + dddddddddddddddddddddddddddddddd_adddddcddddcdb~~~b~~b~~~~~~~~~~~y~~`y~~v~~~v~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~d`_ddddddddddddcdcdddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:63:4725:12502#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; score=239.998975242; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.87095121358; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaaaggaggcgg + ddadcdddcdddddddddcdddaddadTdd\]R\]ddd``dddddd~~~~~~~~~~~~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~d]dWddddddddeddcdcddddddddddddddddcddddddddddd @HELIUM_000100422_612GNAAXX:7:7:11110:17924#0/2_CONS ali_length=62; direction=left; seq_ab_match=58; sminR=40.0; score=215.812113201; seq_a_mismatch=3; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.4808405355; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgaagtagttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtcataccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctactacttcgg + ddddcddd_dddddddddddddddcdddcddddddddcdddddd`d~b~~~~~~~~~~~~~~~~~rm{kripoooEJpoolppp~~~~~~~~~~~~~v~~~~~`v~~~dddcdddddddbddddaddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:102:6364:4627#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; score=251.985885517; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99977596059; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg + ddddddddddddddddddddddddddddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddcddddddddddcdddddddddddddddddcddddddddddddd @HELIUM_000100422_612GNAAXX:7:96:8737:4973#0/2_CONS ali_length=58; direction=right; seq_ab_match=57; sminR=40.0; score=223.980140585; seq_a_mismatch=1; seq_b_deletion=50; seq_b_mismatch=0; seq_a_deletion=50; score_norm=3.86172656181; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=0; seq_b_single=0; ccgcctccttagaacaggctcctctagaaaagcatagtggggtatctaaaggaggcgg + ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @HELIUM_000100422_612GNAAXX:7:89:9710:20652#0/2_CONS ali_length=63; direction=left; seq_ab_match=62; sminR=40.0; score=243.910293117; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.87159195424; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttatatccctctagaggagcctgttctagatattcgg + ddddddddddddd`addddddddd`dddad]ccccddcdddcddd~~}~~~~~~~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~}ddadddddddddddddddddddddddadddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:71:17473:7401#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; score=231.883938289; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.74006352079; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattaccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg + cccccc^ccccccccccccccccc_cccccabaac^ccccc_cc^c~~~~R~~~~s~~w~~~~~xg~~~~powyw~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~~~~dddbddbdccddddaddddbdddddddacdddcddddddddddddd @HELIUM_000100422_612GNAAXX:7:28:9406:18226#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; score=231.816671723; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.73897857618; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg + ddddddddddddddddddddddddddddddddcddddddddddedd_~~~b~~~~~~~~~~~~~~x~~~~~~~~~~{~~~~~~~~~~~~~~|~~~~~~~~~~~~~~~~dcedcdcddddddddddddddddddddcddddeddddddddddddd @HELIUM_000100422_612GNAAXX:7:15:16118:15111#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; score=231.917355899; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.7406025145; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgaagtagttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctactacttcgg + dddddddddddddddddddddddddddddddddddddddddd`d]d~~~c~~~~x~~~~~~a~~~w~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddd`dddedddddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:58:18618:20959#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; score=223.882241304; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=2; seq_a_deletion=0; score_norm=3.611003892; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg + ddddeddddddddddddddddddddddddddd_ddddddcdddddd~~~}b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~|~~~~~z~~x~~~~~~~~~b~~~~~bddddddddddddddddcddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:46:11654:6037#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; score=239.828425953; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.8682004186; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg + ddddddddddbd`adddddddddddddddddddbcbbb^bcddcccb~~~~~~~~~~~~~~~~}~~~~~~~{~~~v~~z{}~~~~~~~~~~~~~~~~~~~~~~~~~~~dcdddddddddcdddedddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:2:6320:8068#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; score=251.910304163; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99857625655; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg + ddedddddddddddddddddddddddddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddeddddbdddddddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:46:1592:11866#0/2_CONS ali_length=62; direction=left; seq_ab_match=62; sminR=40.0; score=247.993604064; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99989683974; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg + ddcdddcddddbdddcdddd_ddaddddd_dddbcd\dddddd]dd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddcddcddd`dddcddcdddddddddddddcdd`dddddcdd`ddd @HELIUM_000100422_612GNAAXX:7:119:2660:19129#0/2_CONS ali_length=63; direction=left; seq_ab_match=61; sminR=40.0; score=235.859804076; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=2; seq_a_deletion=0; score_norm=3.74380641391; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg + ddddcdddedddedddddddddddddddcddcddddddddcdddd~~~~~~~~~~~~~~~~z}~~~~~~~~~~~~~~~~~~~~~~~~~~~~k~~~~~~~~~~b~^~~~dddbddddddddddddddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:115:3625:10051#0/2_CONS ali_length=59; direction=right; seq_ab_match=59; sminR=40.0; score=235.999642017; seq_a_mismatch=0; seq_b_deletion=49; seq_b_mismatch=0; seq_a_deletion=49; score_norm=3.99999393249; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=0; seq_b_single=0; ccaattaacttagataccccactatgccaatactagaggagcctgttctagttaattgg + ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @HELIUM_000100422_612GNAAXX:7:100:17253:5513#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; score=231.761122002; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.73808261294; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg + dddcddcddccddbddddddddbdcdcdddbdddddcddddddddd~~~~|{|~~~~b~~~~~~~~~~s~~~~~x~~~~~~~rs~zqwxy~z~~|}~z~}|{^v~~~~c``Zc`cXWcc[WYYZccccccc_ccccccccWbcc`cc]cccccc @HELIUM_000100422_612GNAAXX:7:82:6036:6921#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; score=231.901981419; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=2; seq_a_deletion=0; score_norm=3.74035453901; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg + dddddddddddddddddddddddddcd`dddbaddddcdccddddd~{~|~~~~~~~~~~~~~~~~~~~}~~~~~~~~]~~~~~~~~~~~~~~~~~~~~~~~b~~~~~dcddddddddeddddbcddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:120:4726:20808#0/2_CONS ali_length=62; direction=left; seq_ab_match=62; sminR=40.0; score=247.810697755; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99694673798; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg + ddddecdcdddddcedddddddddddddbedddddcdddddddddb~~~~~~~~~~~~~~ty}y~~~~~~~~~~~~{~~~~~~~~~~~~~~~~~~~~~~~~~~~~~r~ddddcdddddcdd`dddddedddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:99:8183:13912#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; score=231.903850642; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.74038468777; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctcctagaggagcctgttctagttaattgg + ddddddddddddceeddddddddddddddddddddddcddddddeb~Z~~~~~~~~~~~~~~~~~}~l~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~dadcdedddcdddddddd`ddddddddddddddddcddddeddddd @HELIUM_000100422_612GNAAXX:7:10:4085:7492#0/2_CONS ali_length=63; direction=left; seq_ab_match=62; sminR=40.0; score=243.95581367; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.87231450269; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg + ddddddddddddddddddddddddddddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~ddddddddddddddddddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:35:12723:18557#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; score=251.945522222; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99913527337; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg + ddddddddddddddddd`ddddddddaadddcddddddddddddd~~}~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~cdddcdddddddbddddddcdddddddddddcddddddddddddd @HELIUM_000100422_612GNAAXX:7:97:10495:17046#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; score=239.938979134; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.86998353443; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg + ddedecdddddddbbdddddddddcddddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~a~~~~~~~~~~~~dddddddddddddddcddddddddddedddddcddddddddcdddd @HELIUM_000100422_612GNAAXX:7:73:17068:16620#0/2_CONS ali_length=58; direction=right; seq_ab_match=57; sminR=40.0; score=223.962007947; seq_a_mismatch=0; seq_b_deletion=50; seq_b_mismatch=1; seq_a_deletion=50; score_norm=3.86141393012; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=0; seq_b_single=0; ccgcctccttagaacaggctcctctagaaaagcatagtggggtatctaaaggaggcgg + ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~~~~~~~~~|~ @HELIUM_000100422_612GNAAXX:7:23:13304:5167#0/2_CONS ali_length=62; direction=left; seq_ab_match=62; sminR=40.0; score=247.997553566; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99996054139; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg + dddddddddddddddddddddddddddddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddcdddddddddddddcdddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:54:2542:19180#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; score=239.868258533; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.86884287957; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg + ddddddddddddddddddddddddddddddddbddddcdccddddd~~~~b~~~~~~~~~~~~~~~~~|~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dadbdddddddddddddddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:1:8613:9165#0/2_CONS ali_length=101; direction=left; seq_ab_match=99; sminR=40.0; score=387.825112777; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.83985260176; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=7; seq_b_single=7; ccagagcgagtgggcaatcctgagccaaatctttatttttagaaaaacaaaagggcttcagaaagcaaaaataaacataaaggataggtgcagagactcaatggactcgctctgg + ddddddd~~~~b~~~~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddd @HELIUM_000100422_612GNAAXX:7:109:17917:15159#0/2_CONS ali_length=63; direction=left; seq_ab_match=61; sminR=40.0; score=235.857230348; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.74376556108; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg + dddedddddddddddddddddddddddddddcddddddddddddc~~~~~b~~a~~~~~~~~~~~~~~~~~~~~~}~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddcdddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:59:18371:8149#0/2_CONS ali_length=62; direction=left; seq_ab_match=62; sminR=40.0; score=247.998742319; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99997971482; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg + ddddddddddddddddddddddddddddcddddddddddddddddc~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddedddddddddddddddddcdddddddddddddd @HELIUM_000100422_612GNAAXX:7:1:8670:8750#0/2_CONS ali_length=90; direction=right; seq_ab_match=90; sminR=40.0; score=359.914979278; seq_a_mismatch=0; seq_b_deletion=18; seq_b_mismatch=0; seq_a_deletion=18; score_norm=3.99905532531; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=0; seq_b_single=0; ccgaggcttactaggattagataccctattatttttgaatgttaatgtttgtttgcttgagtagtattagttatgttcttgaaagcctcg + ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @HELIUM_000100422_612GNAAXX:7:86:11013:15234#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; score=239.990009212; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.8708066002; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg + dddddddddddddddddddddddddddddddddddddddddddddd~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddddddddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:77:18518:10758#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; score=223.70605163; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.60816212307; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg + aaa]`_ccca_a`ccccccccc]c_c_caccaccccccccccc_cc~~~~~~~~~~~~wzz{~~a~~~~y~_~~~~~~~~~~m~sygwqv~ccBccccccccccccc~bb^^bc`U\\`\]XPZ\__[\Z`ZX`accccc``c`Rb`Z`bbccc @HELIUM_000100422_612GNAAXX:7:63:18851:7688#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; score=223.854412144; seq_a_mismatch=3; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.61055503459; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg + ddddddddddddddddddedddddddddddddddeddddddddddd~~~~b~~~~~~~~~b~a~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddeddddddddddcdddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:22:1798:3790#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; score=223.82253574; seq_a_mismatch=3; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.61004089903; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagcactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg + ddddddddddddcdddddddddddddddddddadddddddddddddb~~~b~~~~~~~~~~~~~~~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddbddbdadddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:115:14354:10118#0/2_CONS ali_length=63; direction=left; seq_ab_match=59; sminR=40.0; score=219.850378027; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=2; seq_a_deletion=0; score_norm=3.4896885401; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgcgagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg + ddddddddddddddddddddddcdddddddddccddcdddddddd~~~~~b~~~~~~~~~~~~~}~~~~~~}Jt~y|w~~~~~~~~~~~~~~~_~~~~~~~~~~~~b~dcddddeddddddddeddcdddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:99:14794:3606#0/2_CONS ali_length=63; direction=left; seq_ab_match=61; sminR=40.0; score=235.971679218; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.74558220981; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg + dddcdddddddcdddddddcddd``addddddddddddddddddd~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~dddddddddddddddddddddddddddddddcddddddddddddd @HELIUM_000100422_612GNAAXX:7:74:8297:20108#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; score=239.962155962; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.87035735423; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgaagtagttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctactacttcgg + dddddddddddddddddddddddddddddcdddddddddddddd`d~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~}~dddddddddddddddddddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:29:12308:10119#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; score=251.866768717; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99788521773; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg + ddddddddddddddddddddddddddddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddcdeddddedddddddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:25:11691:21134#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; score=223.86269299; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.61068859661; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaaaggaggcgg + dddddddddddddddddddddd`ddcddddddadcdddaddddddd_~~~b~~}~~~~~~~~~~~~~~~}~}~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~d]daddddddcdddddcdddaddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:111:15484:19248#0/2_CONS ali_length=63; direction=left; seq_ab_match=60; sminR=40.0; score=227.828264164; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.6163216534; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg + dddddddddddddddddddddddddddddddcdd`dddddddddd~~~~~b~~~~~~~~s~~~~~~~~`{~~}~xy}~{~~~z~{txvzj~~`~~~~~~~~~~~~~~~dddaddddddddddddddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:103:3594:15996#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; score=231.92194325; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.74067650404; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacacaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg + dddddedddddddddddddddddcddddddddddddddddddddddb~~~~~~~~~~~R~}~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddaddddddddddddd\bddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:1:8732:6209#0/2_PairEnd sminR=40.0; pairend_limit=108; ali_dir=right; score=15.9727840143; mode=joined; sminL=40.0; gctctacagaggcgggggggggggagaaggggaaatcatggggggggggggcgacatctaaaaacatacatacaacccacacctcctaccacacagctcccagcgcaaagatagatcatgatggtaagtagaggcaaatgagatcatctatttttttcaaagagaagacggcatacgatatcggtctcggcattcctgctaaccgctcttccgatc + BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBa[]aa_aaaa]aa`]a\[aZ`\ZcMWR]Z````` @HELIUM_000100422_612GNAAXX:7:1:14576:14181#0/2_CONS ali_length=63; direction=left; seq_ab_match=60; sminR=40.0; score=223.962708229; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.55496362269; seq_b_insertion=0; seq_a_insertion=1; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=46; ccgaagtagttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctactacttcgg + dddddddddddddddddddddddddddddddddddcddddddcda~~~~b~~~~~~~~~~~a~~~~~~`~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddedddddcdddddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:99:13467:17714#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; score=223.932198733; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=2; seq_a_deletion=0; score_norm=3.61180965699; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgcctcctttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctaaggaggcgg + ddddcdddddddddcddddddddddddddcddddddddddddddcd~~~~~~~~b~~~}~~~~~~~~~~~~~~~~~~~~~~~^~~~~~~~~~~~~~~~~~~~~~~~~bddddddddddeddcdddddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:43:7980:7908#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; score=239.984831014; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.87072308087; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg + ddddcdddddddddddd`ddddddddbdaddcdddd_cddcdddda~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~`~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~d`ddddddddddccbdddddddddddddcdddeddddddddddddd @HELIUM_000100422_612GNAAXX:7:105:14135:6905#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; score=239.985379811; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.87073193244; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgcctcctttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcaccagagtactagcggcaacagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctaaggaggcgg + ddddddddddcddedddddddddddddddddddddddddddddddd~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddeddddddddddddbdddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:89:16663:15081#0/2_CONS ali_length=62; direction=left; seq_ab_match=62; sminR=40.0; score=247.939062327; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.9990171343; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg + dddedddddddddddddddddddddddddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddecddddddddddddcdddddcddddddddddddd @HELIUM_000100422_612GNAAXX:7:86:15907:21191#0/2_CONS ali_length=63; direction=left; seq_ab_match=58; sminR=40.0; score=211.91203832; seq_a_mismatch=3; seq_b_deletion=0; seq_b_mismatch=2; seq_a_deletion=0; score_norm=3.36368314793; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg + dddcdddddddddeddddddddaddddbdddcdddddadd\dcdd~b~~~a{~`~~~~~~~~~~~~~~~~~~~[~~~X~~~~~~~~~~~~~~~~~~~~~~~~{~~~~~ddddddddddddded`ddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:81:17261:2872#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; score=251.967130674; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99947826467; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg + dddcddddddddcddddddddddddddddddddeddedddddadd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddedddddddddedddddddddddddddddddddddddddded @HELIUM_000100422_612GNAAXX:7:14:15397:17819#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; score=223.847163266; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.61043811719; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctgttgccgctagtactctggtgaacaattttgtttgtgtaattatttgtgtttagggctaagcatagtggggtatctaaaggaggcgg + ddcddddddddddddddddddddddbddddddcddddddbcddddd~~~~~~~a~~}~~~~~~~~v~~~s~a~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~`~cddddddddddddddddddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:76:18268:14980#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; score=239.985254914; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.87072991797; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttaggggtaagcatagtggggtatctaaaggaggcgg + ddddddddddddddddddddddbdcddbdbddHddcccb^dbdbda~~~~b~~~~~~~~~~~~~~~~~~~~z~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dceddddddddddcdcc\dddddddddddddedddddddddccded @HELIUM_000100422_612GNAAXX:7:16:15509:18069#0/2_CONS ali_length=62; direction=left; seq_ab_match=58; sminR=40.0; score=215.81634366; seq_a_mismatch=4; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.48090876871; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg + dddddddddddddddddcdddd_cdcddbddaLdddddbddddddd_~}}b~~~~~~~~~~~~~}~~~~zz]~~~~~~~~~~~~~~r~a}~~~~~~~~~~~~~~~~~~adddcdbdddbdddddYdcdddcdddddddddddddcddcdddddd @HELIUM_000100422_612GNAAXX:7:25:7789:3090#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; score=223.705772044; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=3; seq_a_deletion=0; score_norm=3.60815761361; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgcctgtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaaaggaggcgg + ddccdcddbdddddddddcdbbddc^T`_`]\L\Zccc^Tb\^abNt|rzu~~~||j|w~~~p~y~}z~~cccBccccccccccccBBc~~~~}~{~~~~~~~~~~~~dbddddddddddddd]daedddddddddddddeddddddddddddd @HELIUM_000100422_612GNAAXX:7:53:18419:8404#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; score=231.841887233; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.73938527796; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg + dddddddddddddedddddddddddddddddd_ddddddedddddd~~~~~~~~~{~~~~~~~~~~~~~~~b~~~~{~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~bddddddddddddddddcedddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:19:11039:3957#0/2_CONS ali_length=62; direction=left; seq_ab_match=62; sminR=40.0; score=247.811165598; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99695428384; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg + dddddddddaddddddddddddddcccdddddbdddddabdddbdd~~~~~~~~~~~~~~~~~~~~~~~v~~~~~~~~~~}~~~~~~~~~~~~~~~~~~~~~~~~~~~dadddddddddddddcdddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:42:19757:3800#0/2_CONS ali_length=62; direction=left; seq_ab_match=57; sminR=40.0; score=187.78301321; seq_a_mismatch=1; seq_b_deletion=1; seq_b_mismatch=3; seq_a_deletion=1; score_norm=3.02875827758; seq_b_insertion=0; seq_a_insertion=1; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=46; ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgtatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg + dddeddddddddddddddddddddddddcdddaddddd`dddddd~~~~~~]~~~~~~~~~~~~~_x~~~~~~~~~~~zv~~~~~~~~~~~`~a~~~|~b~~~~~~~ccdcddddddedddeddddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:116:14244:17926#0/2_CONS ali_length=63; direction=left; seq_ab_match=55; sminR=40.0; score=187.882758912; seq_a_mismatch=5; seq_b_deletion=0; seq_b_mismatch=3; seq_a_deletion=0; score_norm=2.98226601448; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgcaagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg + dddddddddddddddddddddddedddd`dbcccc`dddddeddd~~~~~~_~~~~b~~~~~~^xz{ZRzpooop~~s~~~~~~`~~~~~~~~~~~~~~~~~b~~~b~dddddeddddddddddddddeddddadddddeddddddddddddd @HELIUM_000100422_612GNAAXX:7:91:10071:8960#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; score=251.979171474; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99966938848; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg + dddddddddddddddddddddddddddddddddddddddddeddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddddddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:61:17484:5085#0/2_CONS ali_length=62; direction=left; seq_ab_match=62; sminR=40.0; score=247.996979391; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.9999512805; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgcctcctttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctaaggaggcgg + ddcdddddddddddddddeddddddddddddedddddddddddd`d~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddccddcdddddddddddddddddbddddddddedcd @HELIUM_000100422_612GNAAXX:7:18:2721:16829#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; score=251.960802952; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99937782464; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg + ddddecdededdedddddddddddddddddeeddddedddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dbddddddddddddddddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:2:7296:17275#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; score=223.923525795; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=2; seq_a_deletion=0; score_norm=3.61166977089; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgcctcctttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctaaggaggcgg + ddcddcdd_dddddddddddddcdddddbcacbcbd`ddddddd_b~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~vbdddddddddddcc]baddbddddbdddddddddddddcdddddddd @HELIUM_000100422_612GNAAXX:7:58:8591:17028#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; score=223.788176649; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.60948672015; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaaaggaggcgg + cc^cccccacabcaaccccccccc_\_[__ccaccccc`ac]caaa~~~~_~~`~wx~z~~~~~xxuz{~~~~~~ppppkzzrz~~x~~~~nyw~~~~~y~~a~~~~~ccccccaccbcccccccb_cccccccc_ccccc^]ccccccccccc @HELIUM_000100422_612GNAAXX:7:13:3469:18630#0/2_CONS ali_length=63; direction=left; seq_ab_match=58; sminR=40.0; score=211.840326012; seq_a_mismatch=3; seq_b_deletion=0; seq_b_mismatch=2; seq_a_deletion=0; score_norm=3.36254485733; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg + dddddd`ddadddddddcddddcdddd`ddc_a`^dddd_ddddd~b~~~b~~[~~~~~~~~~~~~~~{yy|{xzsz~~~~~~~~~~~~~~~~~~w{VqLyyx~~~~~ddadcdddadPPQQIdddddddddddddddcddddddd_ddcd`d @HELIUM_000100422_612GNAAXX:7:72:12959:5736#0/2_CONS ali_length=63; direction=left; seq_ab_match=56; sminR=40.0; score=195.664863172; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=7; seq_a_deletion=0; score_norm=3.10579147892; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatctagaacaggctcctctagaggggtgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg + __T__WY_b_Sb__X[_\S_bDDDDDDDDDDDDDDDDDDDDDDDDccccccccccccccccccccBcccccccccBBBcc~_~~~~~~bi~~b~~~~~~~~~~~~~~~dddd^dddddbbbbSb\^b^cacdddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:1:8858:2566#0/2_PairEnd sminR=40.0; pairend_limit=108; ali_dir=left; score=15.7312871104; mode=joined; sminL=40.0; ggctcggagagagggggggggggggggaggggggggatccgggggggggggggtcttacaaaaaaacacaaaacacataaccactctcacacccacactcgcacacccggatgtggttgtggtgtgcgcgtggatggtgtggtgtggtttttttttcaagcagaagacggcatacgagatcggtctcggcattcctgctgaaccgctcttccgatc + BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBa_a_a]aaaa_`Y`_W]Y[S]]aaaabbbbbcbbbbbbbbbbbbb`bbbbbbbbbbbbbb @HELIUM_000100422_612GNAAXX:7:115:6263:17700#0/2_CONS ali_length=63; direction=left; seq_ab_match=61; sminR=40.0; score=235.853550005; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.74370714293; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg + ddddddddddddddddddddddddddddddddddddddddedddd~b~~~b~~~~~~~~~~~~~~~~~~~~~{~~xxy{z|y~~~~~~~~~~~~~~~~~~~~~~~~~~dddedddddddddddcdddddddddddddddcddddddddddddd @HELIUM_000100422_612GNAAXX:7:7:15538:8325#0/2_CONS ali_length=63; direction=left; seq_ab_match=52; sminR=40.0; score=151.662770749; seq_a_mismatch=0; seq_b_deletion=1; seq_b_mismatch=10; seq_a_deletion=0; score_norm=2.40734556744; seq_b_insertion=1; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatctagaacaggctcctctagagggatgtaaagcaccgcccagttctttgagtttcatgctgttgctagtagtactctggccgacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg + __\b\][\XVbZX_\_b[abbDDDDDDDDDDDDDDDDDDDDDDDDcBcccBcccccBccccccBccccccBBccccccccccccccBcca~~~^YgzYxq~~~~}y~~_ccccac_accacba\S_][_cc^cccaccbacba`a_c_`a_aa @HELIUM_000100422_612GNAAXX:7:120:16005:7955#0/2_CONS ali_length=64; direction=left; seq_ab_match=61; sminR=40.0; score=231.880428082; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=2; seq_a_deletion=0; score_norm=3.62313168878; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=44; seq_b_single=44; ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg + dddddddddddddddddddddddddddddddddddddddcdddd~~~~~~~~~~~~~~~~~~`~~~~~~~{~~~~~~~~~~z~~~~~~~~~~~b~~~~~~~~~b~~~~dddddddddddddddddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:115:3396:14235#0/2_CONS ali_length=63; direction=left; seq_ab_match=61; sminR=40.0; score=235.745732131; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.74199574811; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg + ddddddddddddddddddddddddddddddddcdcdddddddddd~~~~~b~~~~~~~~~~~~~~~~~~~s~~~~~~~{~~~~|~ccc~}~}~~~~~~~~~b~~~~~~dddcdddddadddddcdddddddddddddddeddddddddddddd @HELIUM_000100422_612GNAAXX:7:32:15197:9940#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; score=231.86950825; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.73983077822; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg + dddddedddddddddddddddddddeddddddcddddddcdddd`db~~~~~~b~~~~~~~~~~~~~~~{~~~~~}}tw~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddcddddddddddddacddddddddddddcddddddddddddddcd @HELIUM_000100422_612GNAAXX:7:7:17828:13825#0/2_CONS ali_length=62; direction=left; seq_ab_match=56; sminR=40.0; score=187.930205125; seq_a_mismatch=2; seq_b_deletion=1; seq_b_mismatch=3; seq_a_deletion=0; score_norm=3.03113234072; seq_b_insertion=1; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgcctcctttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctaaggaggcgg + ddeddcdddddcddddddddddddddddddbddddddddacbaa[b~b~~~~~~~~~~~~~a~~~~~~~~~~~~b~~~~~~~~~~~b~~~~~~~b}~~~~~~~~~~~_ddddddddddddccdddcdddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:53:2128:13141#0/2_CONS ali_length=62; direction=left; seq_ab_match=62; sminR=40.0; score=247.978630411; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99965532921; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgaagtagttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctactacttcgg + ddcddddddddddddddddddddddddddddddddddddddd^dad~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddcddddddddddcdddddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:1:8644:15229#0/2_CONS ali_length=106; direction=left; seq_ab_match=103; sminR=40.0; score=399.617128202; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=2; seq_a_deletion=0; score_norm=3.76997290756; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=2; seq_b_single=2; cacacgctctccattgagtctctgcacctatcctttttgattttcgctttctgaatctttgtttgttttcggaaaacgtgatttggctcaggattgcccagagcgtgtgg + dd~~~~~~~~~~~~~~~~~~~~~~~~{~~~~b~~~~~~~~~{|~~~~s|~v~~|p~~~~~~~~~~~u~qy~a~~~~~~~x~|~~~~z{~|~o|~{yvwwy~~|~~[~~cb @HELIUM_000100422_612GNAAXX:7:72:7057:20448#0/2_CONS ali_length=62; direction=left; seq_ab_match=62; sminR=40.0; score=247.94833093; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.9991666279; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg + ddddddddddddddddddddddddddddddeddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddebddddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:10:9237:10532#0/2_CONS ali_length=62; direction=left; seq_ab_match=57; sminR=40.0; score=207.90944986; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=3; seq_a_deletion=0; score_norm=3.35337822355; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaattattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaaaggaggcgg + ddcdccddddcddcdcdcdddd[a^[]]V]adadddacdddadddd~~~~b~~b~~~~~~o|~|xpppoj~~Y~~~~~~~~~~~~~~~\~~N~~~~~~~~~~~~~~~~`\_addddddddddd`cSccadddddd`dddddbabdcdddddddd @HELIUM_000100422_612GNAAXX:7:16:16055:12835#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; score=239.868244499; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.86884265322; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg + ddddddddddddddddddddddddddddddddcddddddddddddd~~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~cdddddddddddcddd`bdddddcdddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:17:16877:9760#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; score=223.899159093; seq_a_mismatch=3; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.61127675956; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgcctcctttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctaaggaggcgg + ddddddddddddddddddddeddddcddddddddddddcddddddd~b~~~~~~~~~~~~~a~~~~~~b~~~{~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddddddddddddddddddddeddddddddddddd @HELIUM_000100422_612GNAAXX:7:47:8320:8571#0/2_CONS ali_length=63; direction=left; seq_ab_match=61; sminR=40.0; score=235.809300513; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.74300477004; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg + dddddddddcdddddddddddd`dd`daddd\ddddddaddddad~b~~~~~~~~~~x~~~~~|~~~~~~~~~~~yuu~~~~~~~~~~~~~~~~~~~~~b~~~~~|~~ddddddddddddddddddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:6:6617:2744#0/2_CONS ali_length=63; direction=left; seq_ab_match=61; sminR=40.0; score=235.875132744; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.7440497261; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg + ddddddddddddcadddddddddddddeddddd`ddddddddcbd~b~~~~b~~~~~~~~~~~~|~~~~~~~~y~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddcbddddeddddddddddeddddddddddddd @HELIUM_000100422_612GNAAXX:7:12:9609:14740#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; score=231.833538451; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.73925062018; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg + dddddddeddddddddddddddddddddddddedddddcdddddddb~~~b~~~~~~~~~~~~~~~~~~}~~~~~}~sz~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddcddddddddddcdddddddddddddddddddddddedddd @HELIUM_000100422_612GNAAXX:7:21:2093:4412#0/2_CONS ali_length=63; direction=left; seq_ab_match=60; sminR=40.0; score=227.763131817; seq_a_mismatch=3; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.61528780662; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg + dddddddddddddddddddddddddddeddddddddddddddddd~b~~~~~~~~~~~~~}~~`~~~~~~~~~~~~~Z{~~|{~~~~{~|~|~~~~~}~~~}|~~}}}DDDDDDDD``U`^\[dddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:18:5240:1587#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; score=223.905608015; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=2; seq_a_deletion=0; score_norm=3.61138077443; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg + dddddddddddddddddddddddddddddddddddddddddddddd~}~~~~~~~~~~~~}~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~bdddddddedddddcdddddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:88:15797:19191#0/2_CONS ali_length=62; direction=left; seq_ab_match=58; sminR=40.0; score=215.88300628; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=4; seq_a_deletion=0; score_norm=3.48198397226; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg + ddddddddddddddddddddddddddddddddddddddddddcddc~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~`~~~~b~~b~~~~ddeddddddddddcdddddcddddddddcddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:1:8613:5665#0/2_CONS ali_length=90; direction=right; seq_ab_match=89; sminR=40.0; score=351.879189487; seq_a_mismatch=0; seq_b_deletion=18; seq_b_mismatch=1; seq_a_deletion=18; score_norm=3.90976877208; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=0; seq_b_single=0; ccttcctggggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaaccaggaagg + ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~~~ @HELIUM_000100422_612GNAAXX:7:47:11864:1343#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; score=251.976310174; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99962397102; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg + dddddddddddddddddddddddddddddddddddddddddbdcd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddcdddddddddddddddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:43:8022:8427#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; score=231.869859511; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.73983644372; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg + ddddddddddddddddddddddddddddddddcddddddddddddd~~~~b~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddddcdedddddcdddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:95:11283:2557#0/2_CONS ali_length=58; direction=right; seq_ab_match=57; sminR=40.0; score=223.999078974; seq_a_mismatch=0; seq_b_deletion=50; seq_b_mismatch=1; seq_a_deletion=50; score_norm=3.86205308576; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=0; seq_b_single=0; ccgcctccttagaacaggctcctctagaaaagcatagtggggtatctaaaggaggcgg + ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~a~~~~~~~~~~~~~~~ @HELIUM_000100422_612GNAAXX:7:67:5288:13403#0/2_CONS ali_length=62; direction=left; seq_ab_match=62; sminR=40.0; score=247.999078967; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99998514464; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgcctcctttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctaaggaggcgg + ddeddddddddddddddddddddddddddddddddddcdddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddedddddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:7:11998:4462#0/2_CONS ali_length=63; direction=left; seq_ab_match=59; sminR=40.0; score=219.864332815; seq_a_mismatch=4; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.48991004469; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtagtactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg + dddddddddddddddddddddddddddddddddddddddddcddd~b~~~~~~~~~~~~b~~~~~~~b~~~~~~~G~}}~~}}~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddcdccddedddddddcdddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:116:12598:9964#0/2_CONS ali_length=62; direction=left; seq_ab_match=53; sminR=40.0; score=175.843125508; seq_a_mismatch=8; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=2.83617944368; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg + dddddddddddddddddddddddddddddddddddddddeddddddb~~~b~~~~~~~~~~~b~~~~~~{~b~b~~~~~b~~~~b~~~b~~~~~~~~~~~~~~~~~~`adddbdddddddcddddddddccdcedddeddddddddddbcecdd @HELIUM_000100422_612GNAAXX:7:111:18277:17779#0/2_CONS ali_length=63; direction=left; seq_ab_match=61; sminR=40.0; score=235.877819976; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.74409238057; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctattgctagtagtcctctggcgaattattttgttgtaaaattatctatgtttagggctaagcatagtggggtatctaagatattcgg + dddddddddddddcdddddddddddddddddddddddeddddddd~~~~~b~~~~~~~~~~~~_~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dcdddbddddddddddddeddeddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:85:13257:14594#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; score=231.92296471; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.7406929792; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctgttgccgctagtactctggtgaacaattttgtttgtgtaattatttgtgtttagggctaagcatagtggggtatctaaaggaggcgg + dddddddddddeddddddddddddddddddddacdddddaddccdda~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~_~~~~~~~~~~~~~~~~~~~~~~~ddddddddddeddddddddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:17:11935:17343#0/2_CONS ali_length=63; direction=left; seq_ab_match=62; sminR=40.0; score=243.983570342; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.87275508479; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg + ddddddddddddddddddddddddddddddddddddddddddddd~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddddddcddddddddddddcddddddddddddd @HELIUM_000100422_612GNAAXX:7:38:9654:18700#0/2_CONS ali_length=63; direction=left; seq_ab_match=62; sminR=40.0; score=243.950053136; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.87222306566; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg + dddddddddddddddddddddddddddcddddcddddddddddcd~~~~~~~~~~~~~~~~~~~~~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddeddddddddddddddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:101:11720:19906#0/2_CONS ali_length=62; direction=left; seq_ab_match=57; sminR=40.0; score=207.841888471; seq_a_mismatch=4; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.35228852373; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaaaggaggcgg + dddddddddddddddddddddddddddddddd^ddbbcc^dcdddd_~~~c~~b~~~~x~}~~~~}~~b~}sq~~}t{z~~{~~psh~|~~]~~~~~~~~~~~~~~~~d\ddddddcdddddd_cddddddddddddddddddddddddddcdd @HELIUM_000100422_612GNAAXX:7:42:12961:8650#0/2_CONS ali_length=63; direction=left; seq_ab_match=62; sminR=40.0; score=243.985409859; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.87278428347; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg + ddddddddddddddddddddddddddddddddddddddddddddd~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddcddddddddddddddddddddddddddddeddddddddddddd @HELIUM_000100422_612GNAAXX:7:36:10388:10324#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; score=239.950587748; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.87017077012; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgaagtagttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctactacttcgg + dddddddddddddddddddddddddddddddddddddddddddddd~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddeddddddddddddddddddcddddddddddddd @HELIUM_000100422_612GNAAXX:7:1:8823:6682#0/2_PairEnd sminR=40.0; pairend_limit=108; ali_dir=right; score=15.9839512414; mode=joined; sminL=40.0; gatcgggagagcgtcgtgtaggggaagagggtagatctcggggggggccgtatcattaaaaaaaaaaacaaacatcagcacgaagcacatgacaccgcctagctatcaatcttgagatgagatcagacatctgtttgttttcttttgtttttttttcaagcagaagacggcatacgatatcggtctcggcattcctgctgaaccgctcttccgatc + BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB_aa]a`]^S``aabaab^bbbbab_c``abbbbbbbcbbbbbbbbbbbbbbbbbbbbbbbbbbab @HELIUM_000100422_612GNAAXX:7:66:11860:20419#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; score=239.911387587; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.86953850946; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaaaggaggcgg + ddeddddddddddddedd`dddddd`edddddNddddddddddcdd|~~~b~~~~~~~~~~~~~~~}~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~daddddddddedddddcddddedddddddddddddddddddddedd @HELIUM_000100422_612GNAAXX:7:3:17077:6562#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; score=223.876050008; seq_a_mismatch=3; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.61090403239; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggcagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg + ccccccccccccccccccccccccbccccccccacccccccccccc~~~ua~~~~~~~~~~~~~~}~~~~~~~a~Fz~zz~~~~~~~~~~~~~~~~~~~~~~~~~~~~badadddddddddddcdddddddcddddddddeddddddbdddbdd @HELIUM_000100422_612GNAAXX:7:18:11865:6418#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; score=251.959188225; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99935219405; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg + ddddddddddddddddddddddddddddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddcdddedddddddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:19:4311:13343#0/2_CONS ali_length=63; direction=left; seq_ab_match=58; sminR=40.0; score=211.837822462; seq_a_mismatch=4; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.36250511845; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctcgtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg + dddddddddddcd`ddcddddddcd\bcccdddddddddcdddcb~b~~~b~~~~~|~~~}~~~{y~~_~~~{Z{~~~~~~~~~~~~~~~~~~~b~~~~~~~~~~~~~ddddd^ddcddddcddbdddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:30:1800:7833#0/2_CONS ali_length=62; direction=left; seq_ab_match=52; sminR=40.0; score=167.712615339; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=10; seq_a_deletion=0; score_norm=2.70504218289; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgaagtagttagataccccactatgcttagccctaaacacaagacattaatataacgagattaatcgacagagtactaccggctatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctactacttcgg + DDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDccBccccccccBcBccccBBccBcccccccc~u~v}[wJ~~Q|~~~~~~~~~~~~a~~~~t~Z^ccc`c]bbc_[V_[`[````bb_cc[_cc[\[\[bcbbbccccc @HELIUM_000100422_612GNAAXX:7:16:19299:8684#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; score=231.845804746; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.73944846365; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg + ddddddddddddddddddddddcdddddddddcddddddddddddd~~~~b~~~~~~~`~~~~~~~~~~~~~~~~~~~~~~~~|~~~~~~~~~~~~~~~~~~~~~~~~cadaddddddeddddccdcddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:54:14618:14353#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; score=239.8562981; seq_a_mismatch=1; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.86864996935; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaagttaattgg + ddddddddddddddddddcdddddddddddddcddddddcdddddd~~~~b~~~~~~~~~~~~~~~~~~y~~~~~z|}z~~~~~~~~~~~~~~~~~~~~~~~~~~~~~eddddddddddddddecddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:108:9222:18258#0/2_CONS ali_length=62; direction=left; seq_ab_match=60; sminR=40.0; score=231.857703869; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=2; seq_a_deletion=0; score_norm=3.73964038498; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcagacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctagttaattgg + ddddeeddddddeddddddddddddddddccddddddddddddddd~~~~~~~~~~~~~~~~~~~v~~~~~}~}}~~~~~`~~~~~~~~~~b~~~~~~~~~~~~~~~~dcdddddddddddddddddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:74:4783:20479#0/2_CONS ali_length=62; direction=left; seq_ab_match=61; sminR=40.0; score=239.87314657; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.86892171887; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgcctccttagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaaaggaggcgg + dddddddddddddddddd`dddcddcdadcddWdddddadWddbbcqy~~~~~~~~~~r~}~~~qx~~~~~z|~~~~~~~~~~~~~~~}~~~~~~~~~~~~~~~~~b~d`dZddddbddddddaddddddcddddbdddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:38:3005:20881#0/2_CONS ali_length=63; direction=left; seq_ab_match=60; sminR=40.0; score=227.901935995; seq_a_mismatch=3; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.61749104754; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgttcatgtttagggctaagcatagtggggtatctaagatattcgg + dddddddddddddddddddddddddddddddddbdddddcdddbd~~}~~b~~b~~~~~~|~~~~~~~a~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddeadddddddddddddddddddcddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:4:10139:2389#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; score=251.802206044; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99686041339; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg + ddddddcddddddddddddd`dcdddddaddaddddbdddddddc~~~~~~~~~{~~~~~~~~zxy~~|~~~~ru~~~~~~~~~~~~~~~~~~{~~p~~~~~~~~~~~ddddddddddabaP`ddddabddddddddddddddddcdcdaddd @HELIUM_000100422_612GNAAXX:7:75:9674:16966#0/2_CONS ali_length=58; direction=right; seq_ab_match=57; sminR=40.0; score=224.000075421; seq_a_mismatch=0; seq_b_deletion=50; seq_b_mismatch=1; seq_a_deletion=50; score_norm=3.86207026589; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=0; seq_b_single=0; ccgcctccttagaacaggctcctctagaaaagcatagtggggtatctaaaggaggcgg + ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~~~~~~~~~~~ @HELIUM_000100422_612GNAAXX:7:59:15367:8376#0/2_CONS ali_length=62; direction=left; seq_ab_match=62; sminR=40.0; score=247.996374666; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99994152687; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgaagtagttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctactacttcgg + ddddddddddddddddddddddddddddddddddddddddddddad~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddedddddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:29:1328:13185#0/2_CONS ali_length=63; direction=left; seq_ab_match=59; sminR=40.0; score=223.94591652; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=4; seq_a_deletion=0; score_norm=3.55469708763; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg + ddddddddddddccdddddddddeddddddddddddddddedadd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~W[~~~~~~~~~~~~~~~~~~~~~~~b~~~b~dddcdddddddcddddddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:30:16081:4486#0/2_CONS ali_length=63; direction=left; seq_ab_match=62; sminR=40.0; score=243.950667893; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.87223282369; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatctagaacaggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg + ddddddddddddddddddddddddddddddddddddddcdddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~c~~~dddeddddddddddadddddddddddddddddddddddddddddd @HELIUM_000100422_612GNAAXX:7:104:10274:7999#0/2_CONS ali_length=63; direction=left; seq_ab_match=63; sminR=40.0; score=251.962499638; seq_a_mismatch=0; seq_b_deletion=0; seq_b_mismatch=0; seq_a_deletion=0; score_norm=3.99940475616; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=45; seq_b_single=45; ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg + dddcdddddddddddedddddddddddccdddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddd^ddddddddddddddddddddddddcddddddddddddd @HELIUM_000100422_612GNAAXX:7:14:7706:12338#0/2_CONS ali_length=62; direction=left; seq_ab_match=59; sminR=40.0; score=223.864093999; seq_a_mismatch=2; seq_b_deletion=0; seq_b_mismatch=1; seq_a_deletion=0; score_norm=3.61071119354; seq_b_insertion=0; seq_a_insertion=0; mode=alignment; sminL=40.0; seq_a_single=46; seq_b_single=46; ccgaagtagtagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaactacttcgg + dddcddcddcdbdbddddddddddddddddddcdddddccaddddd~~~~~~b~~~~~~~~~~~x~~~~wyZq~mv~}}~~~~~~~~~P~~~~~~~~~~~~~~~~~~~d^cddddddddcdddadddddddddddddddddddddddddddddd