view test-data/output_ngsfilter.fastq @ 3:e614dcabd870 draft default tip

"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/obitools commit 5d3c7a7b3f7d687bb03ef7993ddf1a6507d655bd"
author iuc
date Mon, 10 May 2021 19:34:04 +0000
parents aca6a8c9cf83
children
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line source

@HELIUM_000100422_612GNAAXX:7:119:14871:19157#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=47; sminR=40.0; seq_b_mismatch=13; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=1; mid_quality=69.1492537313; seq_a_deletion=0; seq_b_single=46; score=115.761290673; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=68.8701298701; sminL=40.0; seq_a_single=46; score_norm=1.86711759151; seq_b_insertion=1; 
ttagccctaaacacaagtaattattataacaaaatcattcgccagagtactaccggcaatagctcaaaactcaaaggacttggcggtgctttataccctt
+
cacdddddddddddddc\d~b~~~b~~~~~~b`ryK~|uxyXk`}~ccBBccBcccBcBcccBcBccccccc~~~~b|~~xdbaddaaWcccdaadddda
@HELIUM_000100422_612GNAAXX:7:108:5640:3823#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=2; seq_a_mismatch=0; tail_quality=66.9; seq_b_deletion=0; mid_quality=78.447761194; seq_a_deletion=0; seq_b_single=46; score=231.881364714; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.9545454545; sminL=40.0; seq_a_single=46; score_norm=3.74002201152; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattgttcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
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dddcdddddcddddddd]d~b~~~~~~~~~~~~~Z~~~~~~~~~~~~~~~~~~~~~~~~~~~}~~~~~~~~~~~~~~~~~~ddddddddddddddddddd
@HELIUM_000100422_612GNAAXX:7:97:14311:19299#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=52; sminR=40.0; seq_b_mismatch=5; seq_a_mismatch=5; tail_quality=67.0; seq_b_deletion=0; mid_quality=75.0820895522; seq_a_deletion=0; seq_b_single=46; score=167.91734192; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.6; avg_quality=74.0064935065; sminL=40.0; seq_a_single=46; score_norm=2.70834422451; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaagagcttaaaactcaaaggacttggcggtgctttataccctt
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d`cdddddcd`ddddaa\b~`~~~~~~~~b~~~~]~b~V~~~~~~~~~~~~~~~~`~~^Zs~r|~~~st~|z|v||^~~|TY``accL]`\`daZddddd
@HELIUM_000100422_612GNAAXX:7:22:8540:14708#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=3; seq_a_mismatch=0; tail_quality=66.9; seq_b_deletion=0; mid_quality=78.2537313433; seq_a_deletion=0; seq_b_single=46; score=223.982011431; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.1; avg_quality=76.7922077922; sminL=40.0; seq_a_single=46; score_norm=3.61261308759; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
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addbddddddddddddedd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~b~~~~~~~~b~~~~ddddddddddabbb^bddd
@HELIUM_000100422_612GNAAXX:7:57:18459:16145#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=3; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.6917293233; seq_a_deletion=0; seq_b_single=45; score=227.883189945; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=66.9; avg_quality=77.1568627451; sminL=40.0; seq_a_single=45; score_norm=3.61719349119; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
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dddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~c~~~~~~~~~~~~~~b~~~~~~b~dddddcdddddddddddd
@HELIUM_000100422_612GNAAXX:7:89:10281:5930#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.5373134328; seq_a_deletion=0; seq_b_single=46; score=231.893859399; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.038961039; sminL=40.0; seq_a_single=46; score_norm=3.7402235387; seq_b_insertion=0; 
ttagccctaaacacaaataattacacaaacaaaattgttcaccagagtactagcggcaacagcttaaaactcaaaggacttggcggtgctttataccctt
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dddddddcddddddcdddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~bddddddceddedd\ddddd
@HELIUM_000100422_612GNAAXX:7:29:15520:18035#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=77.828358209; seq_a_deletion=0; seq_b_single=46; score=239.965360923; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=65.6; avg_quality=76.3311688312; sminL=40.0; seq_a_single=46; score_norm=3.87040904714; seq_b_insertion=0; 
ttagccctaaacacaagtaattattataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
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__`_Y_`_a_`cc_ccccc~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~~~ddddddddddddddddddd
@HELIUM_000100422_612GNAAXX:7:6:9274:14951#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=2; seq_a_mismatch=1; tail_quality=67.1; seq_b_deletion=0; mid_quality=78.2089552239; seq_a_deletion=0; seq_b_single=46; score=223.791171196; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.9; avg_quality=76.7532467532; sminL=40.0; seq_a_single=46; score_norm=3.60953501929; seq_b_insertion=0; 
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
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dddcddddddddddddddd~~~~~~~~~~~~~~~~~~|z~}a|~~~~}}}}~~~~~~~~~~}~}b~b~~~~~~~~~~~~~~ddddddcdcddcd^ddddd
@HELIUM_000100422_612GNAAXX:7:111:2893:6772#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.7164179104; seq_a_deletion=0; seq_b_single=46; score=239.959941063; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.1948051948; sminL=40.0; seq_a_single=46; score_norm=3.87032163005; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
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ddddddddedddddded_d~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~x~dddddddcdddcdcdddad
@HELIUM_000100422_612GNAAXX:7:89:10621:20317#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=3; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=77.5820895522; seq_a_deletion=0; seq_b_single=46; score=223.827654468; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.2077922078; sminL=40.0; seq_a_single=46; score_norm=3.61012345916; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
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dd`ddddadadddddddRc~}~~z~~~~~~~t~sw}~~g}~~~~~~~~~~~~~~~~~~~~~~~~~~~~^~b`~~~~~~~~~ddddddddbdbdd_dddad
@HELIUM_000100422_612GNAAXX:7:50:2791:11138#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.3805970149; seq_a_deletion=0; seq_b_single=46; score=231.976017167; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.9; avg_quality=76.8961038961; sminL=40.0; seq_a_single=46; score_norm=3.74154866398; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
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dbdbdddddddc\ddddbd~~~~~~~~~~~~~~~~~~b~~~^~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddbddddd
@HELIUM_000100422_612GNAAXX:7:108:17701:18887#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=58; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=4; tail_quality=66.9; seq_b_deletion=0; mid_quality=77.6492537313; seq_a_deletion=0; seq_b_single=46; score=215.828321765; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.1; avg_quality=76.2012987013; sminL=40.0; seq_a_single=46; score_norm=3.48110196396; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
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ddec`dddddddddddc^c~~~~~~~~~~~~~~xss~~~~~~~~~~~|{|z{~~{~~~b~~~~~b~~~~~~~~~~~b~~~bdddddd\ddddddcddddd
@HELIUM_000100422_612GNAAXX:7:97:10414:9929#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.8134328358; seq_a_deletion=0; seq_b_single=46; score=239.864541102; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.2792207792; sminL=40.0; seq_a_single=46; score_norm=3.86878292101; seq_b_insertion=0; 
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
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ddddddddddddddedddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~ddddddcddddddcddddd
@HELIUM_000100422_612GNAAXX:7:97:9091:15775#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=3; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.276119403; seq_a_deletion=0; seq_b_single=46; score=223.861916171; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.8116883117; sminL=40.0; seq_a_single=46; score_norm=3.61067606728; seq_b_insertion=0; 
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
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dcdaddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~x~~~~~~~~~|~~~~~~~b~~~~~~^~~b~~~~dddddddddddddcddddd
@HELIUM_000100422_612GNAAXX:7:74:8683:20738#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=62; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.0977443609; seq_a_deletion=0; seq_b_single=45; score=243.961428864; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.5163398693; sminL=40.0; seq_a_single=45; score_norm=3.87240363276; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
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dddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~`~~~~~~~~~~~~~~ddeddddddddddddddd
@HELIUM_000100422_612GNAAXX:7:24:16230:12703#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=77.4552238806; seq_a_deletion=0; seq_b_single=46; score=223.86908915; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.3; avg_quality=76.0519480519; sminL=40.0; seq_a_single=46; score_norm=3.61079176049; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatcccctt
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bdd_ddddaddcbdcdd^b~~~~~b~~~~~~~~~~~~~~~~~~~~~~~~u~y~~~~~~a~~~~~~~~~~~~~~~~{bs~}}a`ddd`a_ddd``Ob`cdd
@HELIUM_000100422_612GNAAXX:7:4:4214:9434#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=58; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=3; tail_quality=63.3; seq_b_deletion=0; mid_quality=76.2388059701; seq_a_deletion=0; seq_b_single=46; score=215.812703412; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=65.3; avg_quality=74.6883116883; sminL=40.0; seq_a_single=46; score_norm=3.48085005502; seq_b_insertion=0; 
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactacaggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
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dddbdddddcddddddddd~^~~~{~{~~~~~}~~{~~ym~L||~~wuy~zyxPzjyyvxku~~~~~~~~~~~~~~~~~xbacdddddaddac_^`_`dc
@HELIUM_000100422_612GNAAXX:7:12:17693:3451#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.8507462687; seq_a_deletion=0; seq_b_single=46; score=247.862243; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.3116883117; sminL=40.0; seq_a_single=46; score_norm=3.9977781129; seq_b_insertion=0; 
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
dedcddddddddddedddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~}v{}~{~}~~~~~~~~~~~~~~~~~~~~~~~~~cdddddcddddddaddddd
@HELIUM_000100422_612GNAAXX:7:3:14624:12375#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=61; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.7669172932; seq_a_deletion=0; seq_b_single=45; score=235.922125108; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.2287581699; sminL=40.0; seq_a_single=45; score_norm=3.74479563663; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
+
dcdcdddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~Y`~~~~~~~~~~~~~~~~~~~~~~~dddddcdddddddceddd
@HELIUM_000100422_612GNAAXX:7:18:5317:11571#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.7462686567; seq_a_deletion=0; seq_b_single=46; score=239.862078449; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.2207792208; sminL=40.0; seq_a_single=46; score_norm=3.86874320079; seq_b_insertion=0; 
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
ddddddddddddddeddde~~~~~~~~~~~~~~~~~~~~~~~~~~~~y~~~~~~~~~~~~~~~~~{~~~~~~~~~~b~~~~dddddcdddddddaddddd
@HELIUM_000100422_612GNAAXX:7:109:17335:11429#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=56; sminR=40.0; seq_b_mismatch=6; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=73.1278195489; seq_a_deletion=0; seq_b_single=45; score=195.716358124; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=66.9; avg_quality=72.3202614379; sminL=40.0; seq_a_single=45; score_norm=3.10660885911; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
+
dddcdaddddddddcdbd~b~~~~~~~~~|~``}~\`~|[~ccccccccccccccccc~z~~s~x~~~~~}~~}}W~~}x~ddddc]``]\Rd`ddb`^
@HELIUM_000100422_612GNAAXX:7:7:10646:8487#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=77.671641791; seq_a_deletion=0; seq_b_single=46; score=231.85443709; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.2857142857; sminL=40.0; seq_a_single=46; score_norm=3.739587695; seq_b_insertion=0; 
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
dccdddddddddddddddec~~~~~~~~~~~~~~~~~~~~~~~~~~~{vztippoph~~~~~~~b~~~~~~~~~~~~~~~~ddddddddddddddddddc
@HELIUM_000100422_612GNAAXX:7:19:4865:20715#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.5074626866; seq_a_deletion=0; seq_b_single=46; score=231.923942025; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.012987013; sminL=40.0; seq_a_single=46; score_norm=3.74070874234; seq_b_insertion=0; 
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
ddcbddddddddddddddb~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~ybddddddddddcddadddcd
@HELIUM_000100422_612GNAAXX:7:13:6954:13039#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.0; seq_a_deletion=0; seq_b_single=45; score=243.872474161; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.431372549; sminL=40.0; seq_a_single=45; score_norm=3.87099165336; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagaggactactagcaatagcttaaaactcaaaggacttggcggtgctttatatccct
+
dddcdddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~r~~~~~~~~~~~~~~~~~~~~~~~b~~~~~dddddddddddddcdddd
@HELIUM_000100422_612GNAAXX:7:120:14963:1929#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.1492537313; seq_a_deletion=0; seq_b_single=46; score=223.810433242; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.7012987013; sminL=40.0; seq_a_single=46; score_norm=3.60984569745; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
dddadddddddedddddbd~b~~}~~~~~~~~~~~~~~~~u~~|~~}~~}~~~}z`}}~~~}~~~~~~~~~~~~~~_~~~~dddddddddddddcddddd
@HELIUM_000100422_612GNAAXX:7:115:3921:10649#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=61; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.5864661654; seq_a_deletion=0; seq_b_single=45; score=235.871015095; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.0718954248; sminL=40.0; seq_a_single=45; score_norm=3.74398436659; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
+
ddedddddddddcddddd~~~~~~~~~~~~~b~~~~~~~~~~~~~~~~~}~}~n{~i~~~~~~~~~~~~~~~~~~~~~~b~dddddddddddddddddd
@HELIUM_000100422_612GNAAXX:7:109:12848:8882#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.3805970149; seq_a_deletion=0; seq_b_single=46; score=231.869965661; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.9025974026; sminL=40.0; seq_a_single=46; score_norm=3.73983815582; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
ddeedddddddddddcd`d~~~~~~~~~~~~~~~~~~~~~~~~~~~~~{~~x~b~~~}~~~~~~~~~~~~~~~~~~~~~~bdcdddddddddddTddddd
@HELIUM_000100422_612GNAAXX:7:84:11257:18886#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=55; sminR=40.0; seq_b_mismatch=8; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=77.1804511278; seq_a_deletion=0; seq_b_single=45; score=187.890540948; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=75.8496732026; sminL=40.0; seq_a_single=45; score_norm=2.98238953885; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
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dddcdddddddddddddd~b~a~~b~~~~b~~~~~~{b~|a~~`\}s~y~~~~~~t~~~~~~~~~~~~~~~~~~~~x~~t~dddddddddddddddddc
@HELIUM_000100422_612GNAAXX:7:72:13060:5051#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.671641791; seq_a_deletion=0; seq_b_single=46; score=239.985823237; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.8; avg_quality=77.1428571429; sminL=40.0; seq_a_single=46; score_norm=3.87073908447; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
ddddddddddddddddd_b~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddeddddd
@HELIUM_000100422_612GNAAXX:7:35:3798:1896#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=15a_F730814; experiment=wolf_diet; forward_tag=gaagtag; reverse_tag=gaagtag; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=66.9; seq_b_deletion=0; mid_quality=78.4701492537; seq_a_deletion=0; seq_b_single=46; score=239.863223783; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.974025974; sminL=40.0; seq_a_single=46; score_norm=3.86876167392; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
dddddddcddbddddddad~b~~}~~~~~~~~~~~~~~~~~n~~~~~|~}~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddcddaddaddd^dddbc
@HELIUM_000100422_612GNAAXX:7:117:12470:18434#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=59; sminR=40.0; seq_b_mismatch=4; seq_a_mismatch=0; tail_quality=66.9; seq_b_deletion=0; mid_quality=78.045112782; seq_a_deletion=0; seq_b_single=45; score=219.83660627; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=66.9; avg_quality=76.5882352941; sminL=40.0; seq_a_single=45; score_norm=3.4894699408; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
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dddddddddcddddddcd~~~~~~~~~~~~~~~~~~~~~~~~~~~~O~~~~~~~~~{~~{~~Wx~~~~~~~~~~~b~~~b}ddabdddddddddddddd
@HELIUM_000100422_612GNAAXX:7:11:8428:13716#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=15a_F730814; experiment=wolf_diet; forward_tag=gaagtag; reverse_tag=gaagtag; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.7985074627; seq_a_deletion=0; seq_b_single=46; score=239.97387484; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.2662337662; sminL=40.0; seq_a_single=46; score_norm=3.87054636839; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
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dddddddddddddddddad~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddddddd
@HELIUM_000100422_612GNAAXX:7:78:18994:16521#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.6940298507; seq_a_deletion=0; seq_b_single=46; score=239.927346216; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.8; avg_quality=77.1623376623; sminL=40.0; seq_a_single=46; score_norm=3.86979590672; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
cddddddcdddddddddad~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~bddaddeeaddded`ddddb
@HELIUM_000100422_612GNAAXX:7:16:5047:7843#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.3007518797; seq_a_deletion=0; seq_b_single=45; score=251.979285107; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.6928104575; sminL=40.0; seq_a_single=45; score_norm=3.99967119217; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
+
dedddbdddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddcdcddddddeec
@HELIUM_000100422_612GNAAXX:7:118:13803:15594#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=60; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.5112781955; seq_a_deletion=0; seq_b_single=45; score=227.87720055; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.0065359477; sminL=40.0; seq_a_single=45; score_norm=3.61709842144; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
+
edd^ddddbdddddeddd~~~~~~~~~~~~~~~~~~a~~~~~~~~~~~{~y~~~~~~~~~~v~~~~~~~~~~b~~b~~~~~dddddddddddddddddd
@HELIUM_000100422_612GNAAXX:7:53:14379:5922#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.0; seq_a_deletion=0; seq_b_single=46; score=247.982071703; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.9; avg_quality=77.4350649351; sminL=40.0; seq_a_single=46; score_norm=3.99971083392; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
ddddddddddddddddd`d~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddeddddd
@HELIUM_000100422_612GNAAXX:7:52:10700:16508#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=64.5; seq_b_deletion=0; mid_quality=77.8805970149; seq_a_deletion=0; seq_b_single=46; score=239.790995416; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.3051948052; sminL=40.0; seq_a_single=46; score_norm=3.86759670025; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
c^ccccccccccacccccc~~~~~~~~~~~~~~{{~~v~~~~~~~~~~~~~~~~}}}~~~~~{~~~~|v|||~}~~~u~~bddddcdbdddbddIddddd
@HELIUM_000100422_612GNAAXX:7:69:7696:12881#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.3082706767; seq_a_deletion=0; seq_b_single=45; score=251.983052061; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.6993464052; sminL=40.0; seq_a_single=45; score_norm=3.99973098509; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
+
eddddddcdddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddcddd
@HELIUM_000100422_612GNAAXX:7:44:12484:5320#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.1; seq_b_deletion=0; mid_quality=78.5149253731; seq_a_deletion=0; seq_b_single=46; score=231.837799589; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.7; avg_quality=77.0064935065; sminL=40.0; seq_a_single=46; score_norm=3.73931934821; seq_b_insertion=0; 
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
deddddddddddddddddc~~~~~~~~~~~~~~~~~~~}}}z~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~adcddddadddddddddddd
@HELIUM_000100422_612GNAAXX:7:21:4713:10229#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.7313432836; seq_a_deletion=0; seq_b_single=46; score=239.864510201; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.2077922078; sminL=40.0; seq_a_single=46; score_norm=3.86878242259; seq_b_insertion=0; 
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
dcdbddddddddcdddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~|~~~~~~~~~~~~~~~~~~~b~~~~~~~~~~~~~~ddddddddddddd`ddddb
@HELIUM_000100422_612GNAAXX:7:38:6201:12003#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=59; sminR=40.0; seq_b_mismatch=4; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=67.7443609023; seq_a_deletion=0; seq_b_single=45; score=219.686655506; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=63.2; avg_quality=67.3986928105; sminL=40.0; seq_a_single=45; score_norm=3.48708976993; seq_b_insertion=0; 
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaatagcttaaaactcaaagaactgggcggtgctttatatcccg
+
dbddcddcddd[dddddc~~~~b~~~~~~~t~}{axzyz{||zzwzr~y}}}}}z~x~cccccccccccccccccBcccBcDDDDDDDDDDDDDDDDaH
@HELIUM_000100422_612GNAAXX:7:33:16506:1490#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.2; seq_b_deletion=0; mid_quality=79.0373134328; seq_a_deletion=0; seq_b_single=46; score=247.963002972; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.1; avg_quality=77.4935064935; sminL=40.0; seq_a_single=46; score_norm=3.99940327375; seq_b_insertion=0; 
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
ddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddeddddd
@HELIUM_000100422_612GNAAXX:7:32:4360:3451#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=61; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.7703703704; seq_a_deletion=0; seq_b_single=47; score=243.999335649; seq_a_insertion=0; mode=alignment; seq_length_ori=155; head_quality=67.0; avg_quality=77.2516129032; sminL=40.0; seq_a_single=47; score_norm=3.999989109; seq_b_insertion=0; 
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
dddddddddddddddddde~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddeddddddddddeedddd
@HELIUM_000100422_612GNAAXX:7:115:17402:4174#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=3; tail_quality=67.1; seq_b_deletion=0; mid_quality=77.9402985075; seq_a_deletion=0; seq_b_single=46; score=223.877240886; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.525974026; sminL=40.0; seq_a_single=46; score_norm=3.6109232401; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactacctgcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
edddddccddddedcdd]d~~~~~~~~~~~~~~~~~~~~~~~~~~~~~s~|y~vS~~~~~~{~~~~~~~~~~~~~~b~~~bdddddddddddddcddddd
@HELIUM_000100422_612GNAAXX:7:45:6831:6228#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.0298507463; seq_a_deletion=0; seq_b_single=46; score=247.974637619; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.4675324675; sminL=40.0; seq_a_single=46; score_norm=3.99959092934; seq_b_insertion=0; 
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
dddcddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddeddddd
@HELIUM_000100422_612GNAAXX:7:117:4291:18111#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=62; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.0827067669; seq_a_deletion=0; seq_b_single=45; score=243.911625561; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.5032679739; sminL=40.0; seq_a_single=45; score_norm=3.87161310415; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
+
ddddddddaddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~dddddddddddddddddd
@HELIUM_000100422_612GNAAXX:7:71:14800:3222#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.5597014925; seq_a_deletion=0; seq_b_single=46; score=231.89389474; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.0584415584; sminL=40.0; seq_a_single=46; score_norm=3.74022410871; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
dddddddeeddddddddcd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~}~~~\~~~~~~~~~~~~~~~~~b~~~~ddddddccdddddcdddde
@HELIUM_000100422_612GNAAXX:7:11:8547:18238#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.2932330827; seq_a_deletion=0; seq_b_single=45; score=251.984413754; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.6862745098; sminL=40.0; seq_a_single=45; score_norm=3.99975259927; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
+
dbdcdddddddddcdddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddddddd
@HELIUM_000100422_612GNAAXX:7:117:2416:10342#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.2781954887; seq_a_deletion=0; seq_b_single=45; score=235.921215944; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=76.8039215686; sminL=40.0; seq_a_single=45; score_norm=3.74478120546; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
+
d^dUYZ[Yddddcdcddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~}~~~~~b|~~b~dddddbddddbddddddd
@HELIUM_000100422_612GNAAXX:7:78:7151:19928#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=56; sminR=40.0; seq_b_mismatch=7; seq_a_mismatch=0; tail_quality=47.6; seq_b_deletion=0; mid_quality=58.2105263158; seq_a_deletion=0; seq_b_single=45; score=195.576462373; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=52.9; avg_quality=57.1699346405; sminL=40.0; seq_a_single=45; score_norm=3.10438829164; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacgagaatgttcgccagtgtacttctagcaacaggctgaaactcaaaggacttggcggtggtttacatccct
+
WSR]YbbbDDDDDDDDDDcccccccccccBBcccccccccccccBcccccBccccccccccBccccccccccBccccc~`~WNS]Y_KTUVZ[O_SHW]
@HELIUM_000100422_612GNAAXX:7:71:18821:20471#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=53; sminR=40.0; seq_b_mismatch=2; seq_a_mismatch=7; tail_quality=66.9; seq_b_deletion=0; mid_quality=75.9029850746; seq_a_deletion=0; seq_b_single=46; score=175.889514148; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=74.7402597403; sminL=40.0; seq_a_single=46; score_norm=2.83692764755; seq_b_insertion=0; 
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
dd\ddddddddd^ddd`dd~~~~Xx~~~~~{|q}}x}^Y`J`~Xv{wt~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~sbddddddddddddd^bdccd
@HELIUM_000100422_612GNAAXX:7:58:15837:8531#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.2857142857; seq_a_deletion=0; seq_b_single=45; score=251.96128051; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.6797385621; sminL=40.0; seq_a_single=45; score_norm=3.99938540492; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
+
dddbdddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddcddddd
@HELIUM_000100422_612GNAAXX:7:38:10813:16867#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.2406015038; seq_a_deletion=0; seq_b_single=45; score=251.97777927; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.6405228758; sminL=40.0; seq_a_single=45; score_norm=3.99964729; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgtttgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
+
ddddddddeddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dbcdddddadadcddddd
@HELIUM_000100422_612GNAAXX:7:68:19115:18847#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=54; sminR=40.0; seq_b_mismatch=2; seq_a_mismatch=6; tail_quality=66.6; seq_b_deletion=0; mid_quality=76.0074626866; seq_a_deletion=0; seq_b_single=46; score=183.856571062; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.7; avg_quality=74.7922077922; sminL=40.0; seq_a_single=46; score_norm=2.96542856552; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
ddecdd_ddcdd_dd^ddd~`~~~~~b~~~~~~~~~~~}~~{b~~~~~~~{~~_~~~s~~s~~~~x~~\\~~~~~~b~~{bd`dcc`P^bb^dbaaaddb
@HELIUM_000100422_612GNAAXX:7:14:3978:11028#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=77.9029850746; seq_a_deletion=0; seq_b_single=46; score=231.869517243; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.8; avg_quality=76.474025974; sminL=40.0; seq_a_single=46; score_norm=3.73983092328; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactacctgcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
ddeadbdcddddeddd`ad~~~~~~~~~~~~~~~~~~~~~~~~~~~~~w|{u~|J~y~~~v~~~~~~~~~~~~~~~~~~~_dddddddddddbbZ`bcdd
@HELIUM_000100422_612GNAAXX:7:34:18733:3827#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.1052631579; seq_a_deletion=0; seq_b_single=45; score=243.983236898; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.522875817; sminL=40.0; seq_a_single=45; score_norm=3.87274979204; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
+
dddddddddddddddddd~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddddddd
@HELIUM_000100422_612GNAAXX:7:65:6787:8456#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.5895522388; seq_a_deletion=0; seq_b_single=46; score=231.869006954; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.8; avg_quality=77.0714285714; sminL=40.0; seq_a_single=46; score_norm=3.7398226928; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
dddddddcddddddedd_d~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~bddddddddddddddddddd
@HELIUM_000100422_612GNAAXX:7:111:7441:14688#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.015037594; seq_a_deletion=0; seq_b_single=45; score=243.832560428; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.4444444444; sminL=40.0; seq_a_single=45; score_norm=3.87035810203; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
+
dddadddddddddedddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~}~~~~~~~~~~~~~~~~~~u~~~~~b~~~~~dddddddddddddddddd
@HELIUM_000100422_612GNAAXX:7:86:10092:15563#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=2; tail_quality=66.8; seq_b_deletion=0; mid_quality=78.1940298507; seq_a_deletion=0; seq_b_single=46; score=223.927744132; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.6; avg_quality=76.7012987013; sminL=40.0; seq_a_single=46; score_norm=3.61173780858; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
ddbdddddccdddddaddd~b~~~~~~~~~~~~~_~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~|bddddddddddcddcddd`^
@HELIUM_000100422_612GNAAXX:7:16:4418:5128#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=3; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.3656716418; seq_a_deletion=0; seq_b_single=46; score=223.862543287; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.8896103896; sminL=40.0; seq_a_single=46; score_norm=3.61068618206; seq_b_insertion=0; 
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
dddeddddddddddddddb~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~{~~~~~~~~~~~~~~~b~~b~~~bdddeddbdddddddddddd
@HELIUM_000100422_612GNAAXX:7:13:5742:9859#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=4; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=58; seq_ab_match=55; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=3; tail_quality=93.0; seq_b_deletion=50; mid_quality=91.4210526316; seq_a_deletion=50; seq_b_single=0; score=207.846895043; seq_a_insertion=0; mode=alignment; seq_length_ori=58; head_quality=89.4; avg_quality=91.3448275862; sminL=40.0; seq_a_single=0; score_norm=3.58356715591; seq_b_insertion=0; 
tttt
+
~~~~
@HELIUM_000100422_612GNAAXX:7:85:18986:17662#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=15a_F730814; experiment=wolf_diet; forward_tag=gaagtag; reverse_tag=gaagtag; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=2; seq_a_mismatch=0; tail_quality=67.1; seq_b_deletion=0; mid_quality=78.447761194; seq_a_deletion=0; seq_b_single=46; score=231.874929984; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.9; avg_quality=76.961038961; sminL=40.0; seq_a_single=46; score_norm=3.73991822554; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
ddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~}~~s~~~~~b~~~~~~b~~~~~~~ddcddddddcddddddddd
@HELIUM_000100422_612GNAAXX:7:16:16357:19948#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=57; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=5; tail_quality=66.7; seq_b_deletion=0; mid_quality=75.6541353383; seq_a_deletion=0; seq_b_single=45; score=203.860719115; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=62.0; avg_quality=74.1764705882; sminL=40.0; seq_a_single=45; score_norm=3.23588443039; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcacaggacttggcggtgctttacatccct
+
dd_dbddbdaddbddddd~`~~b~~~~~~b~~{~~~}x}}~}`twyhw~~~~}w|~|~~~~~X~~~|~~~~~Y~~~~~~~~XVRSQ[Qadd``^P^adY
@HELIUM_000100422_612GNAAXX:7:107:3570:18147#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.0820895522; seq_a_deletion=0; seq_b_single=46; score=231.924768153; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=65.9; avg_quality=76.5714285714; sminL=40.0; seq_a_single=46; score_norm=3.74072206698; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcataaaactcaaaggacttggcggtgctttataccctt
+
dddadddddcddddd\d\d~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~}~~~~~~~~a~s~`ddcd_ddcddddd]ddc_b
@HELIUM_000100422_612GNAAXX:7:82:8836:11354#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.2835820896; seq_a_deletion=0; seq_b_single=46; score=231.781218203; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.8181818182; sminL=40.0; seq_a_single=46; score_norm=3.73840674522; seq_b_insertion=0; 
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
ddedddddddddddddddd~~~~~~{~~~~~~~~~~~~~~~~~~~~~~w{{k~~|~~~~~~~~~~~~~~~~~~~~~b~~~bddddddacdddddcdddda
@HELIUM_000100422_612GNAAXX:7:41:2354:1449#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=55; sminR=40.0; seq_b_mismatch=3; seq_a_mismatch=5; tail_quality=66.4; seq_b_deletion=0; mid_quality=75.5789473684; seq_a_deletion=0; seq_b_single=45; score=187.777927438; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=66.6; avg_quality=74.3921568627; sminL=40.0; seq_a_single=45; score_norm=2.98060202283; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
+
daaddddddcddbcdddd~b}bb~b~~~b~q~~}~}~x~x~t~s~|k|~w~~z~~{|{~ccBc~~}~~~~~~a~~a~}p~~baceddbbedddda_aa_
@HELIUM_000100422_612GNAAXX:7:114:4511:17596#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=57; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=4; tail_quality=67.0; seq_b_deletion=0; mid_quality=75.3208955224; seq_a_deletion=0; seq_b_single=46; score=207.883730153; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.2; avg_quality=74.2532467532; sminL=40.0; seq_a_single=46; score_norm=3.35296338956; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtgctaccggcaatagcttaaaactcaaaggacttggcggtgctttatacgctt
+
ddddddddddddddd^d^d~~~~~~~~~~~~~~~X~~~~~~~~~~~~~W}~~~~~a~sa~~~~~~~~~~~~~~~~~b~~~~ddddddQPPPPPQPKKPPP
@HELIUM_000100422_612GNAAXX:7:94:12367:17729#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=3; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.0; seq_a_deletion=0; seq_b_single=46; score=223.888596169; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.5714285714; sminL=40.0; seq_a_single=46; score_norm=3.61110638983; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
ddd`ddddddddddeddad~~~~~~~~~~~~~~~~~~~~~~~b~~~~~~~~r|~t||x~~~~~~~~~~~~~~~~~}b~~~bcddddd`dbdadccddecc
@HELIUM_000100422_612GNAAXX:7:30:5374:18213#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.3233082707; seq_a_deletion=0; seq_b_single=45; score=251.958026467; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.7124183007; sminL=40.0; seq_a_single=45; score_norm=3.99933375344; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
+
dddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddddddd
@HELIUM_000100422_612GNAAXX:7:49:11740:5547#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=62; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=0; tail_quality=66.6; seq_b_deletion=0; mid_quality=79.1127819549; seq_a_deletion=0; seq_b_single=45; score=243.959827817; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=66.6; avg_quality=77.477124183; sminL=40.0; seq_a_single=45; score_norm=3.87237821931; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
+
eddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~cddddddddcdddddddd
@HELIUM_000100422_612GNAAXX:7:29:3531:8314#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.8571428571; seq_a_deletion=0; seq_b_single=45; score=235.837856208; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.3071895425; sminL=40.0; seq_a_single=45; score_norm=3.74345803504; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
+
ddddddddddddddecdd~~~~~~~~~~~~~~~~~~~~~}~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~a~dcddddddddddcddddd
@HELIUM_000100422_612GNAAXX:7:114:10615:11217#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=1; tail_quality=66.9; seq_b_deletion=0; mid_quality=73.7985074627; seq_a_deletion=0; seq_b_single=46; score=231.702876359; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.7; avg_quality=72.8896103896; sminL=40.0; seq_a_single=46; score_norm=3.73714316708; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
dddddaaa``a_adddd^d~b~~z~~~~~~~~~~~~~cBcccccccccccccc~y~~s~~~~~~~|~~~~~|~~~~sz~~~adddddd[dad`VRW]VZd
@HELIUM_000100422_612GNAAXX:7:108:2729:8600#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.2462686567; seq_a_deletion=0; seq_b_single=46; score=223.856778095; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.7857142857; sminL=40.0; seq_a_single=46; score_norm=3.61059319508; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
ddddddddddddedcdcad~~~~~~~~~~~~~~~a~~~~~~~~~~~~~~~~~~~~~~~`~~~~~~~~~~~}~~~~~b~~~~dddddddcddddWdddddd
@HELIUM_000100422_612GNAAXX:7:103:8847:6326#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.3082706767; seq_a_deletion=0; seq_b_single=45; score=251.968264217; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.6993464052; sminL=40.0; seq_a_single=45; score_norm=3.99949625741; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
+
dddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddcdddd
@HELIUM_000100422_612GNAAXX:7:70:11798:2668#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=15a_F730814; experiment=wolf_diet; forward_tag=gaagtag; reverse_tag=gaagtag; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.8059701493; seq_a_deletion=0; seq_b_single=46; score=239.988667111; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.2727272727; sminL=40.0; seq_a_single=46; score_norm=3.8707849534; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccttt
+
ddddddddddddddddddd~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dcdddddcddddddddbdd
@HELIUM_000100422_612GNAAXX:7:4:18261:9148#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=61; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.8796992481; seq_a_deletion=0; seq_b_single=45; score=235.937993338; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.1; avg_quality=77.3333333333; sminL=40.0; seq_a_single=45; score_norm=3.7450475133; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
+
eddddddddddddddddd~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~`~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddddddd
@HELIUM_000100422_612GNAAXX:7:29:18416:16620#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=77.4179104478; seq_a_deletion=0; seq_b_single=46; score=231.873033258; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.0649350649; sminL=40.0; seq_a_single=46; score_norm=3.7398876332; seq_b_insertion=0; 
ttagccctaaacacaagtaattattataacaaaattattcgccagagttctaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctg
+
dad\bbbbddcdbdcdd`d~~~~~~~~~~~~~~~~~~~~~~~~~~~~zF||u~s~`~~~~~~~~~~~~~~~~~~~~~~~~~ddddddb^bbb`[Hb`dd\
@HELIUM_000100422_612GNAAXX:7:32:18262:6966#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=0; tail_quality=66.4; seq_b_deletion=0; mid_quality=78.7537313433; seq_a_deletion=0; seq_b_single=46; score=239.975704028; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.1; avg_quality=77.1948051948; sminL=40.0; seq_a_single=46; score_norm=3.87057587142; seq_b_insertion=0; 
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
dddddddcddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~`~~~~~~~~~~~~ddddddddddbdddddddd
@HELIUM_000100422_612GNAAXX:7:110:17877:11976#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=77.8805970149; seq_a_deletion=0; seq_b_single=46; score=223.833250149; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.4675324675; sminL=40.0; seq_a_single=46; score_norm=3.61021371209; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
dddcddddddddddd^d\b~`~~~~~~~~~~~~~~~~~~~~x~~~~~~~~~~w{xqv~~~~~~~~~~~~~~~~~~~b~~~bdddddddddddddadddd_
@HELIUM_000100422_612GNAAXX:7:97:9416:9155#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.5895522388; seq_a_deletion=0; seq_b_single=46; score=231.872797448; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.0844155844; sminL=40.0; seq_a_single=46; score_norm=3.7398838298; seq_b_insertion=0; 
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
dddedddddddddddddde~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~{~~~~~~b~~~~~~~~~~~~~~~bddaddddddddddeddddd
@HELIUM_000100422_612GNAAXX:7:72:8905:10715#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.2932330827; seq_a_deletion=0; seq_b_single=45; score=251.929473647; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.6862745098; sminL=40.0; seq_a_single=45; score_norm=3.99888053408; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
+
dddcdddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddcddd
@HELIUM_000100422_612GNAAXX:7:48:5296:16375#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.1278195489; seq_a_deletion=0; seq_b_single=45; score=251.994265764; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.5424836601; sminL=40.0; seq_a_single=45; score_norm=3.99990898038; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
+
cddbdddddaddddbddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddd`dbdaadddd
@HELIUM_000100422_612GNAAXX:7:53:6550:1163#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.8872180451; seq_a_deletion=0; seq_b_single=45; score=235.996766828; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.3333333333; sminL=40.0; seq_a_single=45; score_norm=3.74598042584; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgtttgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
+
ddaddddddddddddddd~b~~~~~~~~~~~~~~~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddddddd
@HELIUM_000100422_612GNAAXX:7:120:1900:13295#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.1; seq_b_deletion=0; mid_quality=77.5149253731; seq_a_deletion=1; seq_b_single=46; score=219.874676069; seq_a_insertion=1; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.1558441558; sminL=40.0; seq_a_single=46; score_norm=3.54636574304; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactacgcgcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
ddcdddddcdddcddddcd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ppthFfpo~~~~~~~~~~~~~~~~~~~b~~~~ddddddcdddddcdddddd
@HELIUM_000100422_612GNAAXX:7:96:19317:7551#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=58; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=3; tail_quality=66.9; seq_b_deletion=0; mid_quality=76.3955223881; seq_a_deletion=0; seq_b_single=46; score=215.85731879; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=65.9; avg_quality=75.0974025974; sminL=40.0; seq_a_single=46; score_norm=3.48156965791; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
ddbb`dddcdddddddd_d~~~~~~~~~~~~~~~Z~~~~~~~~zzt{qx~~yhpppns_|~~~za~~~~|~~~~~~a|~q~cccccccccccbcXcbccc
@HELIUM_000100422_612GNAAXX:7:93:18556:10479#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.3731343284; seq_a_deletion=0; seq_b_single=46; score=223.913795643; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.9; avg_quality=76.8896103896; sminL=40.0; seq_a_single=46; score_norm=3.61151283295; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
ddddddddddddddddddd~b~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~~~dddddddddddddbddddd
@HELIUM_000100422_612GNAAXX:7:44:19605:15509#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=3; tail_quality=67.0; seq_b_deletion=0; mid_quality=77.7313432836; seq_a_deletion=0; seq_b_single=46; score=223.916231984; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.3376623377; sminL=40.0; seq_a_single=46; score_norm=3.61155212878; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
dadcddddddddddd_d_a~~~~~~~~~~~~~~y}z~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~_~~~p~~`k~`~~r~ddcddcadcdcddcddddd
@HELIUM_000100422_612GNAAXX:7:40:3610:8896#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=15a_F730814; experiment=wolf_diet; forward_tag=gaagtag; reverse_tag=gaagtag; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.828358209; seq_a_deletion=0; seq_b_single=46; score=239.967241748; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.2922077922; sminL=40.0; seq_a_single=46; score_norm=3.87043938304; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
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ddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~~~~~dddddddddddddeddddd
@HELIUM_000100422_612GNAAXX:7:41:19763:3556#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=65.7; seq_b_deletion=0; mid_quality=78.5671641791; seq_a_deletion=0; seq_b_single=46; score=247.825851895; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.9805194805; sminL=40.0; seq_a_single=46; score_norm=3.99719115959; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
dddcddddddddddddcad~~~~~~~~~~~~~~~~~~~~~~~~~~~~{m{zj~~~~~~~~v~~~~~~~~~~~~~~~~~~~{dddddddddddddcddddd
@HELIUM_000100422_612GNAAXX:7:111:7660:7465#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=58; sminR=40.0; seq_b_mismatch=3; seq_a_mismatch=2; tail_quality=66.1; seq_b_deletion=0; mid_quality=76.8646616541; seq_a_deletion=0; seq_b_single=45; score=211.768626837; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=66.5; avg_quality=75.4836601307; sminL=40.0; seq_a_single=45; score_norm=3.36140677518; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
+
ddcdddddd`ddddddcd~~~~~~~~_~~~~~~~~~~~~~Ky~~~~~~c~Y}~~s~~`~x~}~}~{~Z|h~~~~~~~~~~~dYYdddaddaaddad^dd
@HELIUM_000100422_612GNAAXX:7:76:10822:18722#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=58; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=5; tail_quality=66.8; seq_b_deletion=0; mid_quality=77.3834586466; seq_a_deletion=0; seq_b_single=45; score=211.83390837; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=66.5; avg_quality=75.9803921569; sminL=40.0; seq_a_single=45; score_norm=3.36244299; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacacgaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
+
acbdcddddTbdcddddd~`~~~z~~~~~~S~~~~~~b~~`~~~t~t~~}~~~~~~~~~~~T~~~|~~~~~~~~~~~~x~~ddddadddd_ddbdcbdd
@HELIUM_000100422_612GNAAXX:7:76:8491:8900#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=57; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=4; tail_quality=65.3; seq_b_deletion=0; mid_quality=76.552238806; seq_a_deletion=0; seq_b_single=46; score=207.828655885; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.9; avg_quality=75.1948051948; sminL=40.0; seq_a_single=46; score_norm=3.35207509492; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtgctacctgcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
bcccccccbcccc_ccb[b~^~~~~~~~~~~~~~uw~~~~}~~~y~~}N~qx~}F}z~~~~~~~~~~~~~o~~~~~b~~~bdddddddddddcdadddda
@HELIUM_000100422_612GNAAXX:7:47:7619:9866#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.1; seq_b_deletion=0; mid_quality=78.8358208955; seq_a_deletion=0; seq_b_single=46; score=239.867362445; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.1; avg_quality=77.3116883117; sminL=40.0; seq_a_single=46; score_norm=3.86882842653; seq_b_insertion=0; 
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
ddddddddddddddddded~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~addddddddddddddddddd
@HELIUM_000100422_612GNAAXX:7:99:14742:5591#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.5895522388; seq_a_deletion=0; seq_b_single=46; score=231.982488051; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.0844155844; sminL=40.0; seq_a_single=46; score_norm=3.74165303308; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
dddddddddddddddddcd~b~~~~~~~~~~~~~a~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddddcdd
@HELIUM_000100422_612GNAAXX:7:95:3274:5435#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=61; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=1; tail_quality=66.8; seq_b_deletion=0; mid_quality=78.8421052632; seq_a_deletion=0; seq_b_single=45; score=235.944669323; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.2810457516; sminL=40.0; seq_a_single=45; score_norm=3.74515348131; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
+
dddcdddddddddcdddd~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~{~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~ddddddddddcddddddd
@HELIUM_000100422_612GNAAXX:7:74:8396:18135#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.8045112782; seq_a_deletion=0; seq_b_single=45; score=235.858013544; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.2614379085; sminL=40.0; seq_a_single=45; score_norm=3.74377799276; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
+
ddddddddddddddcddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~W~dddddddddddddddddd
@HELIUM_000100422_612GNAAXX:7:9:10358:4089#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=61; seq_ab_match=54; sminR=40.0; seq_b_mismatch=6; seq_a_mismatch=1; tail_quality=66.9; seq_b_deletion=1; mid_quality=66.3880597015; seq_a_deletion=0; seq_b_single=47; score=181.620394786; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.9; avg_quality=66.4545454545; sminL=40.0; seq_a_single=46; score_norm=2.97738352108; seq_b_insertion=0; 
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactacaggcaatagctcaaaactcaaagaacttggcggtgctttataccctt
+
d^dadbaddddddcddcd^`zy~XwzzzZ~~~ccccccccccccccccBccccBccccccccccBcccccccccccBcccacc`ddc][\[]]`addddd
@HELIUM_000100422_612GNAAXX:7:78:10338:12302#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.6119402985; seq_a_deletion=0; seq_b_single=46; score=231.881878067; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.9; avg_quality=77.0974025974; sminL=40.0; seq_a_single=46; score_norm=3.74003029141; seq_b_insertion=0; 
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
ddddddddddddddddddd~a~~~~~~~~~~~~~~~~~~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddedddddddddddddddd
@HELIUM_000100422_612GNAAXX:7:95:17941:6113#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=57; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=6; tail_quality=66.1; seq_b_deletion=0; mid_quality=77.9172932331; seq_a_deletion=0; seq_b_single=45; score=203.869927289; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.1; avg_quality=76.4379084967; sminL=40.0; seq_a_single=45; score_norm=3.23603059189; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
+
ddd`dd`cdddc^edeca~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~~~~~~~~~~~~~~~~bb~~~b~~b~~~b~dddddddddddddddddd
@HELIUM_000100422_612GNAAXX:7:59:11971:12063#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.9850746269; seq_a_deletion=0; seq_b_single=46; score=247.995051728; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.4285714286; sminL=40.0; seq_a_single=46; score_norm=3.99992018915; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgtcagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
acddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~|~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddcddddd
@HELIUM_000100422_612GNAAXX:7:47:3878:6141#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.0223880597; seq_a_deletion=0; seq_b_single=46; score=247.947034203; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.461038961; sminL=40.0; seq_a_single=46; score_norm=3.99914571295; seq_b_insertion=0; 
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
ddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddddddd
@HELIUM_000100422_612GNAAXX:7:21:5487:19582#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=58; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=3; tail_quality=67.1; seq_b_deletion=0; mid_quality=77.6641791045; seq_a_deletion=0; seq_b_single=46; score=215.831528178; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.5; avg_quality=76.2532467532; sminL=40.0; seq_a_single=46; score_norm=3.4811536803; seq_b_insertion=0; 
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
dbddcdddcddddddddd_~v}~~}{~~~~b~}ZZ}}}t~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~d`dbdddbdcdcdacdddd
@HELIUM_000100422_612GNAAXX:7:38:13721:2004#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.5298507463; seq_a_deletion=0; seq_b_single=46; score=239.856433937; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.3; avg_quality=76.987012987; sminL=40.0; seq_a_single=46; score_norm=3.86865216027; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
a]ddddddddddddddd`d~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~}}~~~~~~~~~~~~~~~b~~~~~~~~~~~~{~dddddddddbadd^dbddb
@HELIUM_000100422_612GNAAXX:7:111:19168:18517#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=15a_F730814; experiment=wolf_diet; forward_tag=gaagtag; reverse_tag=gaagtag; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=60; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=77.8955223881; seq_a_deletion=0; seq_b_single=46; score=223.825469474; seq_a_insertion=1; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.4805194805; sminL=40.0; seq_a_single=45; score_norm=3.55278522975; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattatccgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
ddbddddddbcdddd^dd~~~{~_~~~~~~~~~y{}{~U~{|~~~~~n~~|~~~~}x~~~~~~~~~~~~~~~~~^~~~~~~ccdaddbdddbddb_dddd
@HELIUM_000100422_612GNAAXX:7:89:16162:8889#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.1278195489; seq_a_deletion=0; seq_b_single=45; score=243.971622168; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.2; avg_quality=77.5555555556; sminL=40.0; seq_a_single=45; score_norm=3.87256543123; seq_b_insertion=0; 
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
dddddddddddddddddd~~c~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddedddddddeddddd
@HELIUM_000100422_612GNAAXX:7:74:4888:6953#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=61; sminR=40.0; seq_b_mismatch=2; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.8571428571; seq_a_deletion=0; seq_b_single=45; score=235.986171514; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.3071895425; sminL=40.0; seq_a_single=45; score_norm=3.74581224625; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
+
dddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~]~~~~~~~~~~~~~~~~~~~dddddddddedddddddd
@HELIUM_000100422_612GNAAXX:7:41:16707:19819#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.3656716418; seq_a_deletion=0; seq_b_single=46; score=231.94731954; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.1; avg_quality=76.8961038961; sminL=40.0; seq_a_single=46; score_norm=3.74108579903; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
dddddddddddddddddbb~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~bddad`a^dddbddaddddd
@HELIUM_000100422_612GNAAXX:7:7:7916:16505#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=4; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=58; seq_ab_match=58; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=93.0; seq_b_deletion=50; mid_quality=93.0; seq_a_deletion=50; seq_b_single=0; score=231.999536374; seq_a_insertion=0; mode=alignment; seq_length_ori=58; head_quality=93.0; avg_quality=93.0; sminL=40.0; seq_a_single=0; score_norm=3.99999200644; seq_b_insertion=0; 
tttt
+
~~~~
@HELIUM_000100422_612GNAAXX:7:41:9316:2256#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.6343283582; seq_a_deletion=0; seq_b_single=46; score=239.993848494; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.1233766234; sminL=40.0; seq_a_single=46; score_norm=3.87086852409; seq_b_insertion=0; 
ttagccctaaacacaagaaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
dddddddddddddddddNd~~~~~~~~~~~~~~~~~~~~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddcadeddddd
@HELIUM_000100422_612GNAAXX:7:67:7582:3642#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=62; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.0902255639; seq_a_deletion=0; seq_b_single=45; score=243.962430842; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.5098039216; sminL=40.0; seq_a_single=45; score_norm=3.87241953718; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
+
dddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~`~~~~~~~~dddddddddddddddddd
@HELIUM_000100422_612GNAAXX:7:42:8435:4155#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.2857142857; seq_a_deletion=0; seq_b_single=45; score=251.987540492; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.1; avg_quality=77.6862745098; sminL=40.0; seq_a_single=45; score_norm=3.99980223003; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
+
ddddddddddd`dddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddddddd
@HELIUM_000100422_612GNAAXX:7:33:9900:9541#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.8955223881; seq_a_deletion=0; seq_b_single=46; score=247.949713348; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.3506493506; sminL=40.0; seq_a_single=46; score_norm=3.99918892497; seq_b_insertion=0; 
ctagccctaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
dddddd\`cccccdddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddedddddddddecddcdd
@HELIUM_000100422_612GNAAXX:7:48:7139:3789#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.3157894737; seq_a_deletion=0; seq_b_single=45; score=251.902451987; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.7058823529; sminL=40.0; seq_a_single=45; score_norm=3.99845161885; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
+
dddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddddddd
@HELIUM_000100422_612GNAAXX:7:46:13269:4134#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.3157894737; seq_a_deletion=0; seq_b_single=45; score=251.997344433; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.7058823529; sminL=40.0; seq_a_single=45; score_norm=3.99995784814; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
+
dddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddcddddddddddd
@HELIUM_000100422_612GNAAXX:7:46:9246:19171#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=52; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=10; tail_quality=65.2; seq_b_deletion=0; mid_quality=70.7611940299; seq_a_deletion=0; seq_b_single=46; score=167.802718427; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.7; avg_quality=70.1363636364; sminL=40.0; seq_a_single=46; score_norm=2.70649545851; seq_b_insertion=0; 
ttagccctaaacacaagtaattattataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
`dddddddddd^bddadcd~b~~~~~b~~~~~~~_`~~~~~b~~~~~S~~~~~~~~~~b~~n~`~~k~zoY|~~~~jb~~~DDDDDDDDDDDDDDDDDD`
@HELIUM_000100422_612GNAAXX:7:37:12979:6595#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=4; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=58; seq_ab_match=58; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=93.0; seq_b_deletion=50; mid_quality=93.0; seq_a_deletion=50; seq_b_single=0; score=231.998796685; seq_a_insertion=0; mode=alignment; seq_length_ori=58; head_quality=93.0; avg_quality=93.0; sminL=40.0; seq_a_single=0; score_norm=3.99997925319; seq_b_insertion=0; 
tttt
+
~~~~
@HELIUM_000100422_612GNAAXX:7:95:1688:9598#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=54; sminR=40.0; seq_b_mismatch=3; seq_a_mismatch=6; tail_quality=66.8; seq_b_deletion=0; mid_quality=74.2932330827; seq_a_deletion=0; seq_b_single=45; score=179.847313607; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=65.7; avg_quality=73.2418300654; sminL=40.0; seq_a_single=45; score_norm=2.8547192636; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtgatactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
+
dddc`ddddcda`bcabb~~[~~~~~~~~~~~~~__~~|z~}}}~~SPF|mxxrwwtIwjxwvv{p~rwxzx`~~Wp~~~~d[ddd`[`^^dcdddd_d
@HELIUM_000100422_612GNAAXX:7:55:16528:11418#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=58; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=4; tail_quality=57.0; seq_b_deletion=0; mid_quality=76.0746268657; seq_a_deletion=0; seq_b_single=46; score=215.988419267; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.5; avg_quality=74.2142857143; sminL=40.0; seq_a_single=46; score_norm=3.48368418172; seq_b_insertion=0; 
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcactagcttacaactcaaaggacttggcggtgctttataccctt
+
ddcdddddddddddcd`da~~~~~~~~~~~~x~~~~~\~~~~~~~~~v~~~~~~~~~~\~~~~~~~[~~~s~~~~~~~~]~cY__]\QPPPP`]`[`c^^
@HELIUM_000100422_612GNAAXX:7:32:18486:7018#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.3233082707; seq_a_deletion=0; seq_b_single=45; score=251.909476689; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.1; avg_quality=77.7189542484; sminL=40.0; seq_a_single=45; score_norm=3.99856312205; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
+
ddddddddddddddedcd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddedddddddd
@HELIUM_000100422_612GNAAXX:7:85:2105:21152#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=57; sminR=40.0; seq_b_mismatch=4; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=73.8947368421; seq_a_deletion=0; seq_b_single=45; score=203.706262238; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=72.9934640523; sminL=40.0; seq_a_single=45; score_norm=3.23343273394; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
+
daddddddddddaddd`d~b~~~~b~s`~b~~~~~~~~~j~x~~~cccccccccccccccccc~~~~\}~}~~~~~{~~b~dbddddedddeddcdddd
@HELIUM_000100422_612GNAAXX:7:83:6795:14061#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.9328358209; seq_a_deletion=0; seq_b_single=46; score=247.992382324; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.3831168831; sminL=40.0; seq_a_single=46; score_norm=3.99987713426; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
ddddddddddddddddd_d~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddcddddddddddbdddbd
@HELIUM_000100422_612GNAAXX:7:73:16525:10764#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.3059701493; seq_a_deletion=0; seq_b_single=46; score=231.90336822; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.9; avg_quality=76.8311688312; sminL=40.0; seq_a_single=46; score_norm=3.74037690678; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
c_dddddddcddcdddd^b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~|~~~~~b~b~~~~~~~~~~~~~~dddddd_bddddb[dddda
@HELIUM_000100422_612GNAAXX:7:19:6313:16567#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=3; tail_quality=67.0; seq_b_deletion=0; mid_quality=77.4135338346; seq_a_deletion=0; seq_b_single=45; score=227.826165596; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=66.6; avg_quality=76.0261437908; sminL=40.0; seq_a_single=45; score_norm=3.61628834279; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaagggcttggcggtgctttacatccct
+
ddddcddddddacddddd~~~~~~~~~~~~~~~~~~~~~}}w|~r~xvwrm{z~~~|a~~~~~~~}u~~}~~~~~_O~~~~ddcddb_b__`baYdcdd
@HELIUM_000100422_612GNAAXX:7:114:8865:19510#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.8059701493; seq_a_deletion=0; seq_b_single=46; score=239.913762365; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.1; avg_quality=77.2792207792; sminL=40.0; seq_a_single=46; score_norm=3.86957681233; seq_b_insertion=0; 
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
ddddddddddddddddddd~a~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddcddddddd
@HELIUM_000100422_612GNAAXX:7:111:8478:1127#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=57; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=5; tail_quality=67.0; seq_b_deletion=0; mid_quality=76.3909774436; seq_a_deletion=0; seq_b_single=45; score=203.794981474; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=66.4; avg_quality=75.1241830065; sminL=40.0; seq_a_single=45; score_norm=3.23484097578; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctggaacgcaaaggacttggcggtgctttacatccct
+
dddaddddddddddMbdd~b~~~|~~~~~~{~~~~~~~zy|~~~~{~pv}y~~|~~~~~~~}~|~HxxwIup`~~Z~~v]~adddddacdddbad^dcd
@HELIUM_000100422_612GNAAXX:7:81:15726:2345#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=56; sminR=40.0; seq_b_mismatch=7; seq_a_mismatch=0; tail_quality=62.3; seq_b_deletion=0; mid_quality=63.1954887218; seq_a_deletion=0; seq_b_single=45; score=195.641193686; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=62.5; avg_quality=63.091503268; sminL=40.0; seq_a_single=45; score_norm=3.10541577279; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaagcactcggcggtgctttacatccct
+
WJXc_bcca_ab__cc_b~~s~~{~~sww~pq~~~~cBccBccccccBcBccccccccccBccccccccccccccBcccBcDDDDDDDDDD`cbQc___
@HELIUM_000100422_612GNAAXX:7:13:14386:20412#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=60; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.4552238806; seq_a_deletion=0; seq_b_single=45; score=223.918527801; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.9675324675; sminL=40.0; seq_a_single=46; score_norm=3.55426234604; seq_b_insertion=1; 
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
decddddddddddddddd~~a~~~~xa~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~t~~~~~~~~~~~~~~~~~~b~~~~ddddddddddddddddddc
@HELIUM_000100422_612GNAAXX:7:83:18882:9967#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=60; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.6466165414; seq_a_deletion=0; seq_b_single=45; score=227.922890965; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.1241830065; sminL=40.0; seq_a_single=45; score_norm=3.6178236661; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
+
dddddddddddddddddd~b~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~~~~~~~~~~ddddddddddddddddda
@HELIUM_000100422_612GNAAXX:7:13:17353:13234#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=2; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=77.6417910448; seq_a_deletion=0; seq_b_single=46; score=223.801902424; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.1; avg_quality=76.2662337662; sminL=40.0; seq_a_single=46; score_norm=3.60970810361; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccttt
+
ddddddddddddeddWd`b~~}~~~~~x~~~~~oVp]~~~~~~~~~~~~~|~~~~~~~~~~~~~~~~~~~~~~~~~~~~~bdaddbddbdddbcUcbddd
@HELIUM_000100422_612GNAAXX:7:53:10153:11845#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=15a_F730814; experiment=wolf_diet; forward_tag=gaagtag; reverse_tag=gaagtag; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.8059701493; seq_a_deletion=0; seq_b_single=46; score=239.985596162; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.2727272727; sminL=40.0; seq_a_single=46; score_norm=3.87073542196; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
ddddddddddddddddddd~~~~~~~~~~~~~~~a~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddeddddd
@HELIUM_000100422_612GNAAXX:7:33:9296:13526#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.5597014925; seq_a_deletion=0; seq_b_single=46; score=239.852481933; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.7; avg_quality=77.038961039; sminL=40.0; seq_a_single=46; score_norm=3.86858841828; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
dcdddddeedddddddd^d~~~~~~~~~~~~~~~~~~~~~~~~~~~~~|~|~~~~~v~~~~~~~~~~~~~~~~~~~~~~~^dddddddbdddddZdddcd
@HELIUM_000100422_612GNAAXX:7:113:2918:18736#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=58; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=4; tail_quality=67.0; seq_b_deletion=0; mid_quality=77.828358209; seq_a_deletion=0; seq_b_single=46; score=215.87931956; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.4220779221; sminL=40.0; seq_a_single=46; score_norm=3.48192450903; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
dcdcdddddddddddd_`d~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~v~~~v~~y`~~y~~~~~~~~~~~b~~b~~~bdcddddcddddda_ddddd
@HELIUM_000100422_612GNAAXX:7:63:4725:12502#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.0671641791; seq_a_deletion=0; seq_b_single=46; score=239.998975242; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.5; avg_quality=76.5974025974; sminL=40.0; seq_a_single=46; score_norm=3.87095121358; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
dcdcddeddddddddWd]d~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~~~~~~~~~~dddddd``ddd]\R]\ddT
@HELIUM_000100422_612GNAAXX:7:7:11110:17924#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=15a_F730814; experiment=wolf_diet; forward_tag=gaagtag; reverse_tag=gaagtag; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=58; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=3; tail_quality=67.0; seq_b_deletion=0; mid_quality=75.723880597; seq_a_deletion=0; seq_b_single=46; score=215.812113201; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.4; avg_quality=74.5519480519; sminL=40.0; seq_a_single=46; score_norm=3.4808405355; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtcataccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
dcddddddddcdddddd`d~b~~~~~~~~~~~~~~~~~rm{kripoooEJpoolppp~~~~~~~~~~~~~v~~~~~`v~~~dddcdddddddbddddadd
@HELIUM_000100422_612GNAAXX:7:102:6364:4627#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.2932330827; seq_a_deletion=0; seq_b_single=45; score=251.985885517; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.6862745098; sminL=40.0; seq_a_single=45; score_norm=3.99977596059; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
+
dddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddcddddddddddcdddd
@HELIUM_000100422_612GNAAXX:7:96:8737:4973#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=4; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=58; seq_ab_match=57; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=93.0; seq_b_deletion=50; mid_quality=92.2631578947; seq_a_deletion=50; seq_b_single=0; score=223.980140585; seq_a_insertion=0; mode=alignment; seq_length_ori=58; head_quality=93.0; avg_quality=92.5172413793; sminL=40.0; seq_a_single=0; score_norm=3.86172656181; seq_b_insertion=0; 
tttt
+
~b~~
@HELIUM_000100422_612GNAAXX:7:89:9710:20652#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=62; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.8421052632; seq_a_deletion=0; seq_b_single=45; score=243.910293117; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.2941176471; sminL=40.0; seq_a_single=45; score_norm=3.87159195424; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttatatccct
+
dad]ccccddcdddcddd~~}~~~~~~~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~}ddaddddddddddddddd
@HELIUM_000100422_612GNAAXX:7:71:17473:7401#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=77.2089552239; seq_a_deletion=0; seq_b_single=46; score=231.883938289; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=65.5; avg_quality=75.7857142857; sminL=40.0; seq_a_single=46; score_norm=3.74006352079; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggtaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
ddddaddddccdbddbddd~~~~~~~~b~~~~~~~~~~~~~~~~~~~~~~~~wywop~~~~gx~~~~~w~~s~~~~R~~~~c^cc_ccccc^caabaccc
@HELIUM_000100422_612GNAAXX:7:28:9406:18226#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.4925373134; seq_a_deletion=0; seq_b_single=46; score=231.816671723; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.0; sminL=40.0; seq_a_single=46; score_norm=3.73897857618; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
ddddddddddddcdcdecd~~~~~~~~~~~~~~~~|~~~~~~~~~~~~~~{~~~~~~~~~~x~~~~~~~~~~~~~~b~~~_ddeddddddddddcddddd
@HELIUM_000100422_612GNAAXX:7:15:16118:15111#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=15a_F730814; experiment=wolf_diet; forward_tag=gaagtag; reverse_tag=gaagtag; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.4104477612; seq_a_deletion=0; seq_b_single=46; score=231.917355899; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.9285714286; sminL=40.0; seq_a_single=46; score_norm=3.7406025145; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
ddddddddddddddd`d]d~~~c~~~~x~~~~~~a~~~w~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddd`dddeddddd
@HELIUM_000100422_612GNAAXX:7:58:18618:20959#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=2; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.2537313433; seq_a_deletion=0; seq_b_single=46; score=223.882241304; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.1; avg_quality=76.7987012987; sminL=40.0; seq_a_single=46; score_norm=3.611003892; seq_b_insertion=0; 
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
ddcddddddddddddddddb~~~~~b~~~~~~~~~x~~z~~~~~|~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b}~~~ddddddcdddddd_ddddd
@HELIUM_000100422_612GNAAXX:7:46:11654:6037#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.4402985075; seq_a_deletion=0; seq_b_single=46; score=239.828425953; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.9545454545; sminL=40.0; seq_a_single=46; score_norm=3.8682004186; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
dddedddcdddddddddcd~~~~~~~~~~~~~~~~~~~~~~~~~~~}{z~~v~~~{~~~~~~~}~~~~~~~~~~~~~~~~bcccddcb^bbbcbdddddd
@HELIUM_000100422_612GNAAXX:7:2:6320:8068#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.3082706767; seq_a_deletion=0; seq_b_single=45; score=251.910304163; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.1; avg_quality=77.7058823529; sminL=40.0; seq_a_single=45; score_norm=3.99857625655; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
+
dddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddeddddbddddddd
@HELIUM_000100422_612GNAAXX:7:46:1592:11866#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=66.5; seq_b_deletion=0; mid_quality=78.6791044776; seq_a_deletion=0; seq_b_single=46; score=247.993604064; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.8; avg_quality=77.1168831169; sminL=40.0; seq_a_single=46; score_norm=3.99989683974; seq_b_insertion=0; 
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
dd_dddbcd\dddddd]dd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddcddcddd`dddcddcdd
@HELIUM_000100422_612GNAAXX:7:119:2660:19129#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=61; sminR=40.0; seq_b_mismatch=2; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.6541353383; seq_a_deletion=0; seq_b_single=45; score=235.859804076; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.1307189542; sminL=40.0; seq_a_single=45; score_norm=3.74380641391; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
+
dcddcddddddddcdddd~~~~~~~~~~~~~~~~z}~~~~~~~~~~~~~~~~~~~~~~~~~~~~k~~~~~~~~~~b~^~~~dddbdddddddddddddd
@HELIUM_000100422_612GNAAXX:7:115:3625:10051#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=5; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=59; seq_ab_match=59; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=93.0; seq_b_deletion=49; mid_quality=93.0; seq_a_deletion=49; seq_b_single=0; score=235.999642017; seq_a_insertion=0; mode=alignment; seq_length_ori=59; head_quality=93.0; avg_quality=93.0; sminL=40.0; seq_a_single=0; score_norm=3.99999393249; seq_b_insertion=0; 
caata
+
~~~~~
@HELIUM_000100422_612GNAAXX:7:100:17253:5513#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=65.1; seq_b_deletion=0; mid_quality=76.6417910448; seq_a_deletion=0; seq_b_single=46; score=231.761122002; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.7; avg_quality=75.2467532468; sminL=40.0; seq_a_single=46; score_norm=3.73808261294; seq_b_insertion=0; 
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
dddbdddddcddddddddd~~~~|{|~~~~b~~~~~~~~~~s~~~~~x~~~~~~~rs~zqwxy~z~~|}~z~}|{^v~~~~c``Zc`cXWcc[WYYZccc
@HELIUM_000100422_612GNAAXX:7:82:6036:6921#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=2; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.4104477612; seq_a_deletion=0; seq_b_single=46; score=231.901981419; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.9285714286; sminL=40.0; seq_a_single=46; score_norm=3.74035453901; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
ddcbddddeddddddddcd~~~~~b~~~~~~~~~~~~~~~~~~~~~~~]~~~~~~~~}~~~~~~~~~~~~~~~~~~~|~{~dddddccdcddddabddd`
@HELIUM_000100422_612GNAAXX:7:120:4726:20808#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.6940298507; seq_a_deletion=0; seq_b_single=46; score=247.810697755; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.9; avg_quality=77.1688311688; sminL=40.0; seq_a_single=46; score_norm=3.99694673798; seq_b_insertion=0; 
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
dbedddddcdddddddddb~~~~~~~~~~~~~~ty}y~~~~~~~~~~~~{~~~~~~~~~~~~~~~~~~~~~~~~~~~~~r~ddddcdddddcdd`ddddd
@HELIUM_000100422_612GNAAXX:7:99:8183:13912#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=1; tail_quality=67.1; seq_b_deletion=0; mid_quality=78.3358208955; seq_a_deletion=0; seq_b_single=46; score=231.903850642; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.8701298701; sminL=40.0; seq_a_single=46; score_norm=3.74038468777; seq_b_insertion=0; 
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctc
+
ddddddddddcddddddeb~Z~~~~~~~~~~~~~~~~~}~l~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~dadcdedddcdddddddd`
@HELIUM_000100422_612GNAAXX:7:10:4085:7492#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=62; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.1052631579; seq_a_deletion=0; seq_b_single=45; score=243.95581367; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.522875817; sminL=40.0; seq_a_single=45; score_norm=3.87231450269; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
+
dddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~dddddddddddddddddd
@HELIUM_000100422_612GNAAXX:7:35:12723:18557#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.1804511278; seq_a_deletion=0; seq_b_single=45; score=251.945522222; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.5882352941; sminL=40.0; seq_a_single=45; score_norm=3.99913527337; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
+
adddcddddddddddddd~~}~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~cdddcdddddddbddddd
@HELIUM_000100422_612GNAAXX:7:97:10495:17046#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=0; tail_quality=66.9; seq_b_deletion=0; mid_quality=78.7686567164; seq_a_deletion=0; seq_b_single=46; score=239.938979134; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.1; avg_quality=77.2402597403; sminL=40.0; seq_a_single=46; score_norm=3.86998353443; seq_b_insertion=0; 
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
ddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~a~~~~~~~~~~~~dddddddddddddddcddd
@HELIUM_000100422_612GNAAXX:7:73:17068:16620#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=4; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=58; seq_ab_match=57; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=0; tail_quality=92.8; seq_b_deletion=50; mid_quality=92.2631578947; seq_a_deletion=50; seq_b_single=0; score=223.962007947; seq_a_insertion=0; mode=alignment; seq_length_ori=58; head_quality=93.0; avg_quality=92.4827586207; sminL=40.0; seq_a_single=0; score_norm=3.86141393012; seq_b_insertion=0; 
tttt
+
~~~~
@HELIUM_000100422_612GNAAXX:7:23:13304:5167#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.0149253731; seq_a_deletion=0; seq_b_single=46; score=247.997553566; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.4545454545; sminL=40.0; seq_a_single=46; score_norm=3.99996054139; seq_b_insertion=0; 
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
ddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddcddddd
@HELIUM_000100422_612GNAAXX:7:54:2542:19180#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.7313432836; seq_a_deletion=0; seq_b_single=46; score=239.868258533; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.2077922078; sminL=40.0; seq_a_single=46; score_norm=3.86884287957; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
dddddddddddddddbdad~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~|~~~~~~~~~~~~~~~~~b~~~~dddddccdcddddbddddd
@HELIUM_000100422_612GNAAXX:7:109:17917:15159#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.8571428571; seq_a_deletion=0; seq_b_single=45; score=235.857230348; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.1; avg_quality=77.3137254902; sminL=40.0; seq_a_single=45; score_norm=3.74376556108; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
+
dddcdddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~}~~~~~~~~~~~~~~~~~~~~~a~~b~~~~~cddddddddddddcdddd
@HELIUM_000100422_612GNAAXX:7:59:18371:8149#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.0149253731; seq_a_deletion=0; seq_b_single=46; score=247.998742319; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.4545454545; sminL=40.0; seq_a_single=46; score_norm=3.99997971482; seq_b_insertion=0; 
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
dcddddddddddddddddc~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddeddddd
@HELIUM_000100422_612GNAAXX:7:86:11013:15234#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.8208955224; seq_a_deletion=0; seq_b_single=46; score=239.990009212; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.2857142857; sminL=40.0; seq_a_single=46; score_norm=3.8708066002; seq_b_insertion=0; 
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
ddddddddddddddddddd~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddddddd
@HELIUM_000100422_612GNAAXX:7:77:18518:10758#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=2; tail_quality=62.5; seq_b_deletion=0; mid_quality=72.2388059701; seq_a_deletion=0; seq_b_single=46; score=223.70605163; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=63.9; avg_quality=71.0649350649; sminL=40.0; seq_a_single=46; score_norm=3.60816212307; seq_b_insertion=0; 
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
caccaccccccccccc_cc~~~~~~~~~~~~wzz{~~a~~~~y~_~~~~~~~~~~m~sygwqv~ccBccccccccccccc~bb^^bc`U\\`\]XPZ\__
@HELIUM_000100422_612GNAAXX:7:63:18851:7688#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=3; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.4104477612; seq_a_deletion=0; seq_b_single=46; score=223.854412144; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.9285714286; sminL=40.0; seq_a_single=46; score_norm=3.61055503459; seq_b_insertion=0; 
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
dcddddddddddedddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~a~b~~~~~~~~~b~~~~dddddddddddeddddddd
@HELIUM_000100422_612GNAAXX:7:22:1798:3790#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=3; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.3208955224; seq_a_deletion=0; seq_b_single=46; score=223.82253574; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.8506493506; sminL=40.0; seq_a_single=46; score_norm=3.61004089903; seq_b_insertion=0; 
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtgctaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
dadbddbdddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~~~~~~~~~~~~~b~~~bdddddddddddddaddddd
@HELIUM_000100422_612GNAAXX:7:115:14354:10118#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=59; sminR=40.0; seq_b_mismatch=2; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.045112782; seq_a_deletion=0; seq_b_single=45; score=219.850378027; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=76.6013071895; sminL=40.0; seq_a_single=45; score_norm=3.4896885401; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactcgcaacagcctgaaactcaaaggacttggcggtgctttacatccct
+
ddeddddddddeddddcd~b~~~~~~~~~~~~_~~~~~~~~~~~~~~~w|y~tJ}~~~~~~}~~~~~~~~~~~~~b~~~~~ddddddddcddccddddd
@HELIUM_000100422_612GNAAXX:7:99:14794:3606#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=61; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.7894736842; seq_a_deletion=0; seq_b_single=45; score=235.971679218; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=66.9; avg_quality=77.2418300654; sminL=40.0; seq_a_single=45; score_norm=3.74558220981; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
+
dddddddddddddddddd~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~dddddddddddddddddd
@HELIUM_000100422_612GNAAXX:7:74:8297:20108#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=15a_F730814; experiment=wolf_diet; forward_tag=gaagtag; reverse_tag=gaagtag; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.776119403; seq_a_deletion=0; seq_b_single=46; score=239.962155962; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.2467532468; sminL=40.0; seq_a_single=46; score_norm=3.87035735423; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
ddcdddddddddddddd`d~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~}~ddddddddddddddddddd
@HELIUM_000100422_612GNAAXX:7:29:12308:10119#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.3233082707; seq_a_deletion=0; seq_b_single=45; score=251.866768717; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.7124183007; sminL=40.0; seq_a_single=45; score_norm=3.99788521773; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
+
dddddddeddddedcddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddddddd
@HELIUM_000100422_612GNAAXX:7:25:11691:21134#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.1567164179; seq_a_deletion=0; seq_b_single=46; score=223.86269299; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.7077922078; sminL=40.0; seq_a_single=46; score_norm=3.61068859661; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
ddcdddddcddddddad]d~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~}~}~~~~~~~~~~~~~~~}~~b~~~_dddddddadddcdaddddd
@HELIUM_000100422_612GNAAXX:7:111:15484:19248#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=60; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=77.954887218; seq_a_deletion=0; seq_b_single=45; score=227.828264164; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=76.522875817; sminL=40.0; seq_a_single=45; score_norm=3.6163216534; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
+
ddddddddddddddaddd~~~~~~~~~~~~~~~`~~jzvxt{~z~~~{~}yx~}~~{`~~~~~~~~s~~~~~~~~b~~~~~dddddddddd`ddcdddd
@HELIUM_000100422_612GNAAXX:7:103:3594:15996#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.3805970149; seq_a_deletion=0; seq_b_single=46; score=231.92194325; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.1; avg_quality=76.9090909091; sminL=40.0; seq_a_single=46; score_norm=3.74067650404; seq_b_insertion=0; 
ctagccttaaacacaaatagttatgcaaacacaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
dddddddddddddddddddb~~~~~~~~~~~R~}~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddaddddd
@HELIUM_000100422_612GNAAXX:7:1:14576:14181#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=15a_F730814; experiment=wolf_diet; forward_tag=gaagtag; reverse_tag=gaagtag; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.5373134328; seq_a_deletion=0; seq_b_single=46; score=223.962708229; seq_a_insertion=1; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.038961039; sminL=40.0; seq_a_single=45; score_norm=3.55496362269; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
ddddddddcddddddcda~~~~b~~~~~~~~~~~a~~~~~~`~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddedddddcddddd
@HELIUM_000100422_612GNAAXX:7:99:13467:17714#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=2; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.3432835821; seq_a_deletion=0; seq_b_single=46; score=223.932198733; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.9; avg_quality=76.8636363636; sminL=40.0; seq_a_single=46; score_norm=3.61180965699; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
ddcddddddddddddddcd~~~~~~~~b~~~}~~~~~~~~~~~~~~~~~~~~~~~^~~~~~~~~~~~~~~~~~~~~~~~~bddddddddddeddcddddd
@HELIUM_000100422_612GNAAXX:7:43:7980:7908#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.5970149254; seq_a_deletion=0; seq_b_single=46; score=239.984831014; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.9; avg_quality=77.0844155844; sminL=40.0; seq_a_single=46; score_norm=3.87072308087; seq_b_insertion=0; 
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
daddcdddd_cddcdddda~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~`~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~d`ddddddddddccbdddd
@HELIUM_000100422_612GNAAXX:7:105:14135:6905#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.8134328358; seq_a_deletion=0; seq_b_single=46; score=239.985379811; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.2792207792; sminL=40.0; seq_a_single=46; score_norm=3.87073193244; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcaccagagtactagcggcaacagcttaaaactcaaaggacttggcggtgctttataccctt
+
ddddddddddddddddddd~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddeddddddddddddbddd
@HELIUM_000100422_612GNAAXX:7:89:16663:15081#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.0149253731; seq_a_deletion=0; seq_b_single=46; score=247.939062327; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.1; avg_quality=77.461038961; sminL=40.0; seq_a_single=46; score_norm=3.9990171343; seq_b_insertion=0; 
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
ddddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddecddddd
@HELIUM_000100422_612GNAAXX:7:86:15907:21191#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=58; sminR=40.0; seq_b_mismatch=2; seq_a_mismatch=3; tail_quality=67.0; seq_b_deletion=0; mid_quality=77.9172932331; seq_a_deletion=0; seq_b_single=45; score=211.91203832; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=66.9; avg_quality=76.4836601307; sminL=40.0; seq_a_single=45; score_norm=3.36368314793; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
+
dd`deddddddddddddd~~~~~{~~~~~~~~~~~~~~~~~~~~~~~~X~~~[~~~~~~~~~~~~~~~~~~~`~{a~~~b~ddcd\ddadddddcdddb
@HELIUM_000100422_612GNAAXX:7:81:17261:2872#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.1; seq_b_deletion=0; mid_quality=79.3157894737; seq_a_deletion=0; seq_b_single=45; score=251.967130674; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=66.9; avg_quality=77.7058823529; sminL=40.0; seq_a_single=45; score_norm=3.99947826467; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
+
dddedddddddddedddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddadddddeddedddddd
@HELIUM_000100422_612GNAAXX:7:14:15397:17819#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.171641791; seq_a_deletion=0; seq_b_single=46; score=223.847163266; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.9; avg_quality=76.7142857143; sminL=40.0; seq_a_single=46; score_norm=3.61043811719; seq_b_insertion=0; 
ttagccctaaacacaaataattacacaaacaaaattgttcaccagagtactagcggcaacagcttaaaactcaaaggacttggcggtgctttataccctt
+
ddddddddddddddddddc~`~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~a~s~~~v~~~~~~~~}~~a~~~~~~~dddddcbddddddcddddd
@HELIUM_000100422_612GNAAXX:7:76:18268:14980#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=66.9; seq_b_deletion=0; mid_quality=78.3208955224; seq_a_deletion=0; seq_b_single=46; score=239.985254914; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.8441558442; sminL=40.0; seq_a_single=46; score_norm=3.87072991797; seq_b_insertion=0; 
ttacccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
d\ccdcddddddddddecd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~z~~~~~~~~~~~~~~~~~~~~b~~~~adbdbd^bcccddHddbdb
@HELIUM_000100422_612GNAAXX:7:16:15509:18069#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=58; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=4; tail_quality=66.8; seq_b_deletion=0; mid_quality=77.5; seq_a_deletion=0; seq_b_single=46; score=215.81634366; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.1233766234; sminL=40.0; seq_a_single=46; score_norm=3.48090876871; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
cdYdddddbdddbdcddda~~~~~~~~~~~~~~~~~~}a~r~~~~~~~~~~~~~~]zz~~~~}~~~~~~~~~~~~~b}}~_dddddddbdddddLaddbd
@HELIUM_000100422_612GNAAXX:7:25:7789:3090#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=3; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=72.4179104478; seq_a_deletion=0; seq_b_single=46; score=223.705772044; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.5; avg_quality=71.6818181818; sminL=40.0; seq_a_single=46; score_norm=3.60815761361; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactacaggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
ead]dddddddddddddbd~~~~~~~~~~~~{~}~~~~cBBccccccccccccBccc~~z}~y~p~~~w|j||~~~uzr|tNba^\bT^cccZ\L\]`_`
@HELIUM_000100422_612GNAAXX:7:53:18419:8404#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.5447761194; seq_a_deletion=0; seq_b_single=46; score=231.841887233; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.0454545455; sminL=40.0; seq_a_single=46; score_norm=3.73938527796; seq_b_insertion=0; 
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
decddddddddddddddddb~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~{~~~~b~~~~~~~~~~~~~~~{~~~~~~~~~ddddddedddddd_ddddd
@HELIUM_000100422_612GNAAXX:7:19:11039:3957#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.8432835821; seq_a_deletion=0; seq_b_single=46; score=247.811165598; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.7; avg_quality=77.2857142857; sminL=40.0; seq_a_single=46; score_norm=3.99695428384; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
dddcdddddddddddddad~~~~~~~~~~~~~~~~~~~~~~~~~~~}~~~~~~~~~~v~~~~~~~~~~~~~~~~~~~~~~~ddbdddbadddddbddddd
@HELIUM_000100422_612GNAAXX:7:42:19757:3800#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=57; sminR=40.0; seq_b_mismatch=3; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=1; mid_quality=77.7669172932; seq_a_deletion=1; seq_b_single=46; score=187.78301321; seq_a_insertion=1; mode=alignment; seq_length_ori=153; head_quality=67.1; avg_quality=76.3660130719; sminL=40.0; seq_a_single=45; score_norm=3.02875827758; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatatacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
ddddedddeddddddcdcc~~~~~~~b~|~~~a~`~~~~~~~~~~~vz~~~~~~~~~~~x_~~~~~~~~~~~~~]~~~~~~dddddd`dddddadddcd
@HELIUM_000100422_612GNAAXX:7:116:14244:17926#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=55; sminR=40.0; seq_b_mismatch=3; seq_a_mismatch=5; tail_quality=67.0; seq_b_deletion=0; mid_quality=76.5338345865; seq_a_deletion=0; seq_b_single=45; score=187.882758912; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=75.2875816993; sminL=40.0; seq_a_single=45; score_norm=2.98226601448; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgcaagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
+
d`dbcccc`dddddeddd~~~~~~_~~~~b~~~~~~^xz{ZRzpooop~~s~~~~~~`~~~~~~~~~~~~~~~~~b~~~b~dddddedddddddddddd
@HELIUM_000100422_612GNAAXX:7:91:10071:8960#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.3233082707; seq_a_deletion=0; seq_b_single=45; score=251.979171474; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.7124183007; sminL=40.0; seq_a_single=45; score_norm=3.99966938848; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
+
ddddddddddddddeddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddddddd
@HELIUM_000100422_612GNAAXX:7:61:17484:5085#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.9776119403; seq_a_deletion=0; seq_b_single=46; score=247.996979391; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.9; avg_quality=77.4155844156; sminL=40.0; seq_a_single=46; score_norm=3.9999512805; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
ddddedddddddddddd`d~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddccddcddd
@HELIUM_000100422_612GNAAXX:7:18:2721:16829#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.3308270677; seq_a_deletion=0; seq_b_single=45; score=251.960802952; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.2; avg_quality=77.7320261438; sminL=40.0; seq_a_single=45; score_norm=3.99937782464; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
+
dddeeddddedddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dbdddddddddddddddd
@HELIUM_000100422_612GNAAXX:7:2:7296:17275#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=2; seq_a_mismatch=1; tail_quality=66.9; seq_b_deletion=0; mid_quality=78.0298507463; seq_a_deletion=0; seq_b_single=46; score=223.923525795; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.3; avg_quality=76.5454545455; sminL=40.0; seq_a_single=46; score_norm=3.61166977089; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
dbcacbcbd`ddddddd_b~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~vbdddddddddddcc]baddb
@HELIUM_000100422_612GNAAXX:7:58:8591:17028#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=2; tail_quality=66.0; seq_b_deletion=0; mid_quality=75.8582089552; seq_a_deletion=0; seq_b_single=46; score=223.788176649; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=65.3; avg_quality=74.5324675325; sminL=40.0; seq_a_single=46; score_norm=3.60948672015; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
_bcccccccbccacccccc~~~~~a~~y~~~~~wyn~~~~x~~zrzzkpppp~~~~~~{zuxx~~~~~z~xw~`~~_~~~~aaac]ca`cccccacc__[
@HELIUM_000100422_612GNAAXX:7:13:3469:18630#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=58; sminR=40.0; seq_b_mismatch=2; seq_a_mismatch=3; tail_quality=66.0; seq_b_deletion=0; mid_quality=76.2631578947; seq_a_deletion=0; seq_b_single=45; score=211.840326012; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=66.3; avg_quality=74.9411764706; sminL=40.0; seq_a_single=45; score_norm=3.36254485733; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
+
dddIQQPPdadddcdadd~~~~~xyyLqV{w~~~~~~~~~~~~~~~~~~zszx{|yy{~~~~~~~~~~~~~~[~~b~~~b~ddddd_dddd^`a_cdd`
@HELIUM_000100422_612GNAAXX:7:72:12959:5736#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=56; sminR=40.0; seq_b_mismatch=7; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=63.5263157895; seq_a_deletion=0; seq_b_single=45; score=195.664863172; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=59.8; avg_quality=63.5098039216; sminL=40.0; seq_a_single=45; score_norm=3.10579147892; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacacccct
+
^\bSbbbbddddd^dddd~~~~~~~~~~~~~~~b~~ib~~~~~~_~ccBBBcccccccccBccccccccccccccccccccDDDDDDDDDDDDDDDDDD
@HELIUM_000100422_612GNAAXX:7:115:6263:17700#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.6390977444; seq_a_deletion=0; seq_b_single=45; score=235.853550005; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.1176470588; sminL=40.0; seq_a_single=45; score_norm=3.74370714293; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
+
ddcdddddddddddeddd~~~~~~~~~~~~~~~~~~~~~~~~~~y|z{yxx~~{~~~~~~~~~~~~~~~~~~~~~b~~~b~ddddeddddddddddddd
@HELIUM_000100422_612GNAAXX:7:7:15538:8325#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=52; sminR=40.0; seq_b_mismatch=10; seq_a_mismatch=0; tail_quality=63.4; seq_b_deletion=1; mid_quality=60.1879699248; seq_a_deletion=0; seq_b_single=45; score=151.662770749; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=59.2; avg_quality=60.3333333333; sminL=40.0; seq_a_single=45; score_norm=2.40734556744; seq_b_insertion=1; 
ttagccctaaacataaacattcaataaacaagaatgtcggccagagtactactagcaacagcatgaaactcaaagaactgggcggtgctttacatccct
+
_S\abcacca_cacccc_~~y}~~~~qxYzgY^~~~accBccccccccccccccBBccccccBccccccBcccccBcccBcDDDDDDDDDDDDDDDDDD
@HELIUM_000100422_612GNAAXX:7:120:16005:7955#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=98; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=64; seq_ab_match=61; sminR=40.0; seq_b_mismatch=2; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.8939393939; seq_a_deletion=0; seq_b_single=44; score=231.880428082; seq_a_insertion=0; mode=alignment; seq_length_ori=152; head_quality=67.0; avg_quality=77.3289473684; sminL=40.0; seq_a_single=44; score_norm=3.62313168878; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagccgaaactcaaaggacttggcggtgctttacatccct
+
ddddddddddddddddd~~~~b~~~~~~~~~b~~~~~~~~~~~z~~~~~~~~~~{~~~~~~~`~~~~~~~~~~~~~~~~~~ddddcdddddddddddd
@HELIUM_000100422_612GNAAXX:7:115:3396:14235#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=61; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.1052631579; seq_a_deletion=0; seq_b_single=45; score=235.745732131; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=76.6535947712; sminL=40.0; seq_a_single=45; score_norm=3.74199574811; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
+
ddcdddddadddddcddd~~~~~~b~~~~~~~~~}~}~ccc~|~~~~{~~~~~~~s~~~~~~~~~~~~~~~~~~~b~~~~~ddddddddddcdcddddd
@HELIUM_000100422_612GNAAXX:7:32:15197:9940#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=66.9; seq_b_deletion=0; mid_quality=78.3656716418; seq_a_deletion=0; seq_b_single=46; score=231.86950825; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.1; avg_quality=76.8896103896; sminL=40.0; seq_a_single=46; score_norm=3.73983077822; seq_b_insertion=0; 
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
ddcaddddddddddddcdd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~wt}}~~~~~{~~~~~~~~~~~~~~~b~~~~~~bd`ddddcddddddcddddd
@HELIUM_000100422_612GNAAXX:7:7:17828:13825#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=56; sminR=40.0; seq_b_mismatch=3; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=1; mid_quality=77.5223880597; seq_a_deletion=0; seq_b_single=46; score=187.930205125; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.1558441558; sminL=40.0; seq_a_single=46; score_norm=3.03113234072; seq_b_insertion=1; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
dddbddddddddacbaa[b~b~~~~~~~~~~~~~a~~~~~~~~~~~~b~~~~~~~~~~~b~~~~~~~b}~~~~~~~~~~~_ddddddddddddccdddcd
@HELIUM_000100422_612GNAAXX:7:53:2128:13141#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=15a_F730814; experiment=wolf_diet; forward_tag=gaagtag; reverse_tag=gaagtag; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.947761194; seq_a_deletion=0; seq_b_single=46; score=247.978630411; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.9; avg_quality=77.3896103896; sminL=40.0; seq_a_single=46; score_norm=3.99965532921; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
ddddddddddddddd^dad~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddcddddddddddcddddd
@HELIUM_000100422_612GNAAXX:7:72:7057:20448#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.0298507463; seq_a_deletion=0; seq_b_single=46; score=247.94833093; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.4675324675; sminL=40.0; seq_a_single=46; score_norm=3.9991666279; seq_b_insertion=0; 
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
dddeddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddebdddd
@HELIUM_000100422_612GNAAXX:7:10:9237:10532#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=57; sminR=40.0; seq_b_mismatch=3; seq_a_mismatch=2; tail_quality=66.9; seq_b_deletion=0; mid_quality=75.9402985075; seq_a_deletion=0; seq_b_single=46; score=207.90944986; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.7; avg_quality=74.7532467532; sminL=40.0; seq_a_single=46; score_norm=3.35337822355; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaataattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
cSc`ddddddddddda_\`~~~~~~~~~~~~~~~~N~~\~~~~~~~~~~~~~~~Y~~jopppx|~|o~~~~~~b~~b~~~~ddddadddcadddada]V]
@HELIUM_000100422_612GNAAXX:7:16:16055:12835#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.7462686567; seq_a_deletion=0; seq_b_single=46; score=239.868244499; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.2207792208; sminL=40.0; seq_a_single=46; score_norm=3.86884265322; seq_b_insertion=0; 
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
db`dddcdddddddddddc~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~dddddddddddddcddddd
@HELIUM_000100422_612GNAAXX:7:17:16877:9760#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=3; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.3731343284; seq_a_deletion=0; seq_b_single=46; score=223.899159093; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.8961038961; sminL=40.0; seq_a_single=46; score_norm=3.61127675956; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
dddddddddddcddddddd~b~~~~~~~~~~~~~a~~~~~~b~~~{~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddddddd
@HELIUM_000100422_612GNAAXX:7:47:8320:8571#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=61; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.4586466165; seq_a_deletion=0; seq_b_single=45; score=235.809300513; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=66.9; avg_quality=76.954248366; sminL=40.0; seq_a_single=45; score_norm=3.74300477004; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
+
dddddddddddddddddd~~|~~~~~b~~~~~~~~~~~~~~~~~~~~~uuy~~~~~~~~~~~|~~~~~x~~~~~~~~~~b~daddddadddddd\ddda
@HELIUM_000100422_612GNAAXX:7:6:6617:2744#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.7593984962; seq_a_deletion=0; seq_b_single=45; score=235.875132744; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.2222222222; sminL=40.0; seq_a_single=45; score_norm=3.7440497261; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
+
eddddd`ddddddddcbd~b~~~~b~~~~~~~~~~~~|~~~~~~~~y~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddddcbdd
@HELIUM_000100422_612GNAAXX:7:12:9609:14740#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.1; seq_b_deletion=0; mid_quality=78.4701492537; seq_a_deletion=0; seq_b_single=46; score=231.833538451; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.1; avg_quality=76.9935064935; sminL=40.0; seq_a_single=46; score_norm=3.73925062018; seq_b_insertion=0; 
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
dcddddddddddcdddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~zs~}~~~~~}~~~~~~~~~~~~~~~~~~b~~~bdddddddcdddddeddddd
@HELIUM_000100422_612GNAAXX:7:21:2093:4412#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=3; tail_quality=67.0; seq_b_deletion=0; mid_quality=76.1428571429; seq_a_deletion=0; seq_b_single=45; score=227.763131817; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=74.9477124183; sminL=40.0; seq_a_single=45; score_norm=3.61528780662; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
+
eddddddddddddddddd~b~~~~~~~~~~~~~}~~`~~~~~~~~~~~~~Z{~~|{~~~~{~|~|~~~~~}~~~}|~~}}}DDDDDDDD``U`^\[ddd
@HELIUM_000100422_612GNAAXX:7:18:5240:1587#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=2; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.3880597015; seq_a_deletion=0; seq_b_single=46; score=223.905608015; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.9090909091; sminL=40.0; seq_a_single=46; score_norm=3.61138077443; seq_b_insertion=0; 
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
ddddddddddddddddddd~}~~~~~~~~~~~~}~~~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~bdddddddedddddcddddd
@HELIUM_000100422_612GNAAXX:7:88:15797:19191#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=58; sminR=40.0; seq_b_mismatch=4; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.1492537313; seq_a_deletion=0; seq_b_single=46; score=215.88300628; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.7012987013; sminL=40.0; seq_a_single=46; score_norm=3.48198397226; seq_b_insertion=0; 
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
dddddddddddddddcddc~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~`~~~~b~~b~~~~ddeddddddddddcddddd
@HELIUM_000100422_612GNAAXX:7:47:11864:1343#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.2857142857; seq_a_deletion=0; seq_b_single=45; score=251.976310174; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.6797385621; sminL=40.0; seq_a_single=45; score_norm=3.99962397102; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
+
ddddddddddddddbdcd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddcddddddddddddddd
@HELIUM_000100422_612GNAAXX:7:43:8022:8427#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.5970149254; seq_a_deletion=0; seq_b_single=46; score=231.869859511; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.0909090909; sminL=40.0; seq_a_single=46; score_norm=3.73983644372; seq_b_insertion=0; 
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
dedcddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~b~~~~dddddddddddddcddddd
@HELIUM_000100422_612GNAAXX:7:95:11283:2557#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=4; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=58; seq_ab_match=57; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=0; tail_quality=93.0; seq_b_deletion=50; mid_quality=92.2368421053; seq_a_deletion=50; seq_b_single=0; score=223.999078974; seq_a_insertion=0; mode=alignment; seq_length_ori=58; head_quality=93.0; avg_quality=92.5; sminL=40.0; seq_a_single=0; score_norm=3.86205308576; seq_b_insertion=0; 
tttt
+
~~~~
@HELIUM_000100422_612GNAAXX:7:67:5288:13403#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=29a_F260619; experiment=wolf_diet; forward_tag=gcctcct; reverse_tag=gcctcct; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.0298507463; seq_a_deletion=0; seq_b_single=46; score=247.999078967; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.1; avg_quality=77.474025974; sminL=40.0; seq_a_single=46; score_norm=3.99998514464; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
ddddddddddcdddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddeddddd
@HELIUM_000100422_612GNAAXX:7:7:11998:4462#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=59; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=4; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.2105263158; seq_a_deletion=0; seq_b_single=45; score=219.864332815; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=76.7450980392; sminL=40.0; seq_a_single=45; score_norm=3.48991004469; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtagtactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
+
ddddddddddddddcddd~b~~~~~~~~~~~~b~~~~~~~b~~~~~~~G~}}~~}}~~~~~~~~~~~~~~~~~~~~~~~~~ddddddddddddcdccdd
@HELIUM_000100422_612GNAAXX:7:116:12598:9964#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=53; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=8; tail_quality=66.7; seq_b_deletion=0; mid_quality=77.0671641791; seq_a_deletion=0; seq_b_single=46; score=175.843125508; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=75.7402597403; sminL=40.0; seq_a_single=46; score_norm=2.83617944368; seq_b_insertion=0; 
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
ddddddcdddddddbddda`~~~~~~~~~~~~~~~~~~b~~~b~~~~b~~~~~b~b~{~~~~~~b~~~~~~~~~~~b~~~bddddddedddddddddddd
@HELIUM_000100422_612GNAAXX:7:111:18277:17779#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.8646616541; seq_a_deletion=0; seq_b_single=45; score=235.877819976; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.3137254902; sminL=40.0; seq_a_single=45; score_norm=3.74409238057; seq_b_insertion=0; 
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaatagcctgaaactcaaaggacttggcggtgctttacatccct
+
ddddddddddddbdddcd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~_~~~~~~~~~~~~b~~~~~dddddddedddddddddd
@HELIUM_000100422_612GNAAXX:7:85:13257:14594#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.5298507463; seq_a_deletion=0; seq_b_single=46; score=231.92296471; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.0324675325; sminL=40.0; seq_a_single=46; score_norm=3.7406929792; seq_b_insertion=0; 
ttagccctaaacacaaataattacacaaacaaaattgttcaccagagtactagcggcaacagcttaaaactcaaaggacttggcggtgctttataccctt
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ddddddddedddddddddd~~~~~~~~~~~~~~~~~~~~~~~_~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~addccddadddddcaddddd
@HELIUM_000100422_612GNAAXX:7:17:11935:17343#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.0902255639; seq_a_deletion=0; seq_b_single=45; score=243.983570342; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.5098039216; sminL=40.0; seq_a_single=45; score_norm=3.87275508479; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
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dddddddddddddddddd~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddddddd
@HELIUM_000100422_612GNAAXX:7:38:9654:18700#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.0902255639; seq_a_deletion=0; seq_b_single=45; score=243.950053136; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.5098039216; sminL=40.0; seq_a_single=45; score_norm=3.87222306566; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
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ddddddddddddddeddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~b~~~~~~~~~~~~~~~~~~~~~~~dcddddddddddcddddc
@HELIUM_000100422_612GNAAXX:7:101:11720:19906#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=57; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=4; tail_quality=66.9; seq_b_deletion=0; mid_quality=76.9104477612; seq_a_deletion=0; seq_b_single=46; score=207.841888471; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=75.6168831169; sminL=40.0; seq_a_single=46; score_norm=3.35228852373; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
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ddc_ddddddcdddddd\d~~~~~~~~~~~~~~~~]~~|~hsp~~{~~z{t}~~qs}~b~~}~~~~}~x~~~~b~~c~~~_ddddcd^ccbbdd^ddddd
@HELIUM_000100422_612GNAAXX:7:42:12961:8650#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.1052631579; seq_a_deletion=0; seq_b_single=45; score=243.985409859; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.522875817; sminL=40.0; seq_a_single=45; score_norm=3.87278428347; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
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dddddddddddddddddd~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddcddddddddddddddd
@HELIUM_000100422_612GNAAXX:7:36:10388:10324#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=15a_F730814; experiment=wolf_diet; forward_tag=gaagtag; reverse_tag=gaagtag; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.8208955224; seq_a_deletion=0; seq_b_single=46; score=239.950587748; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.2857142857; sminL=40.0; seq_a_single=46; score_norm=3.87017077012; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
ddddddddddddddddddd~b~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddeddddd
@HELIUM_000100422_612GNAAXX:7:66:11860:20419#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.1; seq_b_deletion=0; mid_quality=78.5671641791; seq_a_deletion=0; seq_b_single=46; score=239.911387587; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.1; avg_quality=77.0779220779; sminL=40.0; seq_a_single=46; score_norm=3.86953850946; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
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ddcdddddeddddddddad~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~}~~~~~~~~~~~~~~~b~~~|ddcddddddddddNddddd
@HELIUM_000100422_612GNAAXX:7:3:17077:6562#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=3; tail_quality=66.6; seq_b_deletion=0; mid_quality=77.6567164179; seq_a_deletion=0; seq_b_single=46; score=223.876050008; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.0; avg_quality=76.1818181818; sminL=40.0; seq_a_single=46; score_norm=3.61090403239; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactgccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
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dddcdddddddddddadab~~~~~~~~~~~~~~~~~~~~~~~~~~~~zz~zF~a~~~~~~~}~~~~~~~~~~~~~~au~~~ccccccccccccacccccc
@HELIUM_000100422_612GNAAXX:7:18:11865:6418#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.3157894737; seq_a_deletion=0; seq_b_single=45; score=251.959188225; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.7058823529; sminL=40.0; seq_a_single=45; score_norm=3.99935219405; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
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dddddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddcdddeddddddd
@HELIUM_000100422_612GNAAXX:7:19:4311:13343#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=58; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=4; tail_quality=67.0; seq_b_deletion=0; mid_quality=77.7969924812; seq_a_deletion=0; seq_b_single=45; score=211.837822462; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=76.385620915; sminL=40.0; seq_a_single=45; score_norm=3.36250511845; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactacgagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
+
dbddcddddcdd^ddddd~~~~~~~~~~~~~b~~~~~~~~~~~~~~~~~~~{Z{~~~_~~y{~~~}~~~|~~~~~b~~~b~bcdddcdddddddddccc
@HELIUM_000100422_612GNAAXX:7:30:1800:7833#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=15a_F730814; experiment=wolf_diet; forward_tag=gaagtag; reverse_tag=gaagtag; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=52; sminR=40.0; seq_b_mismatch=10; seq_a_mismatch=0; tail_quality=65.6; seq_b_deletion=0; mid_quality=59.9104477612; seq_a_deletion=0; seq_b_single=46; score=167.712615339; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=35.0; avg_quality=58.6623376623; sminL=40.0; seq_a_single=46; score_norm=2.70504218289; seq_b_insertion=0; 
ttagccctaaacacaagacattaatataacgagattaatcgacagagtactaccggctatagcttaaaactcaaaggacttggcggtgctttataccctt
+
DDDDDDDDDDDDDDDDDDDccBccccccccBcBccccBBccBcccccccc~u~v}[wJ~~Q|~~~~~~~~~~~~a~~~~t~Z^ccc`c]bbc_[V_[`[`
@HELIUM_000100422_612GNAAXX:7:16:19299:8684#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.5; seq_a_deletion=0; seq_b_single=46; score=231.845804746; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.0064935065; sminL=40.0; seq_a_single=46; score_norm=3.73944846365; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
cdccddddeddddddadac~~~~~~~~~~~~~~~~~~~~~~~~|~~~~~~~~~~~~~~~~~~~~~~~~`~~~~~~~b~~~~dddddddddddddcddddd
@HELIUM_000100422_612GNAAXX:7:54:14618:14353#0/2_CONS_SUB_SUB_CMP sample=13a_F730603; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=aattaac; reverse_tag=aattaac; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=1; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.6865671642; seq_a_deletion=0; seq_b_single=46; score=239.8562981; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.1688311688; sminL=40.0; seq_a_single=46; score_norm=3.86864996935; seq_b_insertion=0; 
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
ddcedddddddddddddde~~~~~~~~~~~~~~~~~~~~~~~~~~~~~z}|z~~~~~y~~~~~~~~~~~~~~~~~~b~~~~ddddddcddddddcddddd
@HELIUM_000100422_612GNAAXX:7:108:9222:18258#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=13a_F730603; experiment=wolf_diet; forward_tag=aattaac; reverse_tag=aattaac; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=60; sminR=40.0; seq_b_mismatch=2; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.5; seq_a_deletion=0; seq_b_single=46; score=231.857703869; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.2; avg_quality=77.0194805195; sminL=40.0; seq_a_single=46; score_norm=3.73964038498; seq_b_insertion=0; 
ctagccttaaacacaaatagttatgcagacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
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ddccddddddddddddddd~~~~~~~~~~~~~~~~~~~v~~~~~}~}}~~~~~`~~~~~~~~~~b~~~~~~~~~~~~~~~~dcddddddddddddddddd
@HELIUM_000100422_612GNAAXX:7:74:4783:20479#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=61; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=77.9029850746; seq_a_deletion=0; seq_b_single=46; score=239.87314657; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=76.487012987; sminL=40.0; seq_a_single=46; score_norm=3.86892171887; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
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dddaddddddbddddZd`d~b~~~~~~~~~~~~~~~~~}~~~~~~~~~~~~~~~|z~~~~~xq~~~}~r~~~~~~~~~~yqcbbddWdadddddWddcda
@HELIUM_000100422_612GNAAXX:7:38:3005:20881#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=60; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=3; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.5939849624; seq_a_deletion=0; seq_b_single=45; score=227.901935995; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.0784313725; sminL=40.0; seq_a_single=45; score_norm=3.61749104754; seq_b_insertion=0; 
ttagccctaaacatgaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
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ddddddddddddddaedd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~a~~~~~~~|~~~~~~b~~b~~}~~dbdddcdddddbdddddd
@HELIUM_000100422_612GNAAXX:7:4:10139:2389#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=66.5; seq_b_deletion=0; mid_quality=78.4962406015; seq_a_deletion=0; seq_b_single=45; score=251.802206044; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=66.9; avg_quality=76.954248366; sminL=40.0; seq_a_single=45; score_norm=3.99686041339; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
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daddaddddbdddddddc~~~~~~~~~{~~~~~~~~zxy~~|~~~~ru~~~~~~~~~~~~~~~~~~{~~p~~~~~~~~~~~ddddddddddabaP`ddd
@HELIUM_000100422_612GNAAXX:7:75:9674:16966#0/2_CONS_SUB_SUB_CMP sample=29a_F260619; experiment=wolf_diet; seq_length=4; forward_score=72.0; forward_tag=gcctcct; reverse_tag=gcctcct; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=58; seq_ab_match=57; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=0; tail_quality=93.0; seq_b_deletion=50; mid_quality=92.2631578947; seq_a_deletion=50; seq_b_single=0; score=224.000075421; seq_a_insertion=0; mode=alignment; seq_length_ori=58; head_quality=93.0; avg_quality=92.5172413793; sminL=40.0; seq_a_single=0; score_norm=3.86207026589; seq_b_insertion=0; 
tttt
+
~~~~
@HELIUM_000100422_612GNAAXX:7:59:15367:8376#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=100; sample=15a_F730814; experiment=wolf_diet; forward_tag=gaagtag; reverse_tag=gaagtag; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=62; seq_ab_match=62; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.0149253731; seq_a_deletion=0; seq_b_single=46; score=247.996374666; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=67.0; avg_quality=77.4545454545; sminL=40.0; seq_a_single=46; score_norm=3.99994152687; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
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dddddddddddddddddad~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddddddddddeddddd
@HELIUM_000100422_612GNAAXX:7:29:1328:13185#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=59; sminR=40.0; seq_b_mismatch=4; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=78.3007518797; seq_a_deletion=0; seq_b_single=45; score=223.94591652; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=76.8235294118; sminL=40.0; seq_a_single=45; score_norm=3.55469708763; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
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dddddddddddddedadd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~W[~~~~~~~~~~~~~~~~~~~~~~~b~~~b~dddcdddddddcdddddd
@HELIUM_000100422_612GNAAXX:7:30:16081:4486#0/2_CONS_SUB_SUB_CMP sample=26a_F040644; experiment=wolf_diet; seq_length=99; forward_score=72.0; forward_tag=gaatatc; reverse_tag=gaatatc; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=63; seq_ab_match=62; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.0902255639; seq_a_deletion=0; seq_b_single=45; score=243.950667893; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=67.0; avg_quality=77.5098039216; sminL=40.0; seq_a_single=45; score_norm=3.87223282369; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
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dddaddddddddddeddd~~~c~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ddddddcddddddddddd
@HELIUM_000100422_612GNAAXX:7:104:10274:7999#0/2_CONS_SUB_SUB reverse_score=72.0; reverse_match=tagaacaggctcctctag; seq_length=99; sample=26a_F040644; experiment=wolf_diet; forward_tag=gaatatc; reverse_tag=gaatatc; reverse_primer=tagaacaggctcctctag; status=full; forward_score=72.0; direction=forward; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; ali_length=63; seq_ab_match=63; sminR=40.0; seq_b_mismatch=0; seq_a_mismatch=0; tail_quality=67.0; seq_b_deletion=0; mid_quality=79.2556390977; seq_a_deletion=0; seq_b_single=45; score=251.962499638; seq_a_insertion=0; mode=alignment; seq_length_ori=153; head_quality=66.9; avg_quality=77.6470588235; sminL=40.0; seq_a_single=45; score_norm=3.99940475616; seq_b_insertion=0; 
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
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ccdddddddddddddddd~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~dddddd^ddddddddddd
@HELIUM_000100422_612GNAAXX:7:14:7706:12338#0/2_CONS_SUB_SUB_CMP sample=15a_F730814; experiment=wolf_diet; seq_length=100; forward_score=72.0; forward_tag=gaagtag; reverse_tag=gaagtag; forward_match=ttagataccccactatgc; forward_primer=ttagataccccactatgc; reverse_score=72.0; status=full; direction=reverse; reverse_match=tagaacaggctcctctag; reverse_primer=tagaacaggctcctctag; ali_length=62; seq_ab_match=59; sminR=40.0; seq_b_mismatch=1; seq_a_mismatch=2; tail_quality=67.0; seq_b_deletion=0; mid_quality=77.6194029851; seq_a_deletion=0; seq_b_single=46; score=223.864093999; seq_a_insertion=0; mode=alignment; seq_length_ori=154; head_quality=66.7; avg_quality=76.2207792208; sminL=40.0; seq_a_single=46; score_norm=3.61071119354; seq_b_insertion=0; 
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
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dddadddcddddddddc^d~~~~~~~~~~~~~~~~~~~P~~~~~~~~~}}~vm~qZyw~~~~x~~~~~~~~~~~b~~~~~~dddddaccdddddcddddd