annotate scripts/pseudotemporal.R @ 0:e0e9b24d76aa draft

planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/raceid3 commit f880060c478d42202df5b78a81329f8af56b1138
author iuc
date Thu, 22 Nov 2018 04:42:18 -0500
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children c8434a623268
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e0e9b24d76aa planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/raceid3 commit f880060c478d42202df5b78a81329f8af56b1138
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1 #!/usr/bin/env R
e0e9b24d76aa planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/raceid3 commit f880060c478d42202df5b78a81329f8af56b1138
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2 VERSION = "0.1"
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3
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4 args = commandArgs(trailingOnly = T)
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6 if (length(args) != 1){
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7 message(paste("VERSION:", VERSION))
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8 stop("Please provide the config file")
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9 }
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11 suppressWarnings(suppressPackageStartupMessages(require(RaceID)))
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12 source(args[1])
e0e9b24d76aa planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/raceid3 commit f880060c478d42202df5b78a81329f8af56b1138
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14 test <- list()
e0e9b24d76aa planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/raceid3 commit f880060c478d42202df5b78a81329f8af56b1138
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15 test$side = 3
e0e9b24d76aa planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/raceid3 commit f880060c478d42202df5b78a81329f8af56b1138
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16 test$line = 3
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17 second <- test
e0e9b24d76aa planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/raceid3 commit f880060c478d42202df5b78a81329f8af56b1138
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18 second$cex = 0.5
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19 second$line = 2.5
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22 do.pseudotemp <- function(sc){
e0e9b24d76aa planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/raceid3 commit f880060c478d42202df5b78a81329f8af56b1138
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23 pdf(out.pdf)
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24 ltr <- Ltree(sc)
e0e9b24d76aa planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/raceid3 commit f880060c478d42202df5b78a81329f8af56b1138
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25 ltr <- compentropy(ltr)
e0e9b24d76aa planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/raceid3 commit f880060c478d42202df5b78a81329f8af56b1138
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26 ltr <- do.call(projcells, c(ltr, pstc.projc))
e0e9b24d76aa planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/raceid3 commit f880060c478d42202df5b78a81329f8af56b1138
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27 ltr <- do.call(projback, c(ltr, pstc.projb))
e0e9b24d76aa planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/raceid3 commit f880060c478d42202df5b78a81329f8af56b1138
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28 ltr <- lineagegraph(ltr)
e0e9b24d76aa planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/raceid3 commit f880060c478d42202df5b78a81329f8af56b1138
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29 ltr <- do.call(comppvalue, c(ltr, pstc.comppval))
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30 x <- do.call(compscore, c(ltr, pstc.compscore))
e0e9b24d76aa planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/raceid3 commit f880060c478d42202df5b78a81329f8af56b1138
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31 print(do.call(mtext, c("Compute Score", test)))
e0e9b24d76aa planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/raceid3 commit f880060c478d42202df5b78a81329f8af56b1138
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32 print(do.call(mtext, c("No. of inter-cluster links / Delta median entropy of each cluster / StemID2 score (combination of both)", second)))
e0e9b24d76aa planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/raceid3 commit f880060c478d42202df5b78a81329f8af56b1138
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33 plotdistanceratio(ltr)
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34 print(do.call(mtext, c("Cell-to-Cell Distance Ratio", test)))
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35 print(do.call(mtext, c("Original vs High-dimensional Embedded Space", second)))
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36 do.call(plotgraph, c(ltr, pstc.plotgraph))
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37 print(do.call(mtext, c("Lineage Trajectories ", test)))
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38 print(do.call(mtext, c("Colour = Level of Significance, Width = Link Score ", second)))
e0e9b24d76aa planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/raceid3 commit f880060c478d42202df5b78a81329f8af56b1138
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39 plotspantree(ltr)
e0e9b24d76aa planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/raceid3 commit f880060c478d42202df5b78a81329f8af56b1138
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40 print(do.call(mtext, c("Minimum Spanning Tree", test)))
e0e9b24d76aa planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/raceid3 commit f880060c478d42202df5b78a81329f8af56b1138
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41 plotprojections(ltr)
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42 print(do.call(mtext, c("Minimum Spanning Tree", test)))
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43 print(do.call(mtext, c("Cells Projected onto Links", second)))
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44 test$side = 4
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45 test$line = 0
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46 plotlinkscore(ltr)
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47 print(do.call(mtext, c("Link Score", test)))
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48 projenrichment(ltr)
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49 print(do.call(mtext, c("Enrichment Ratios", test)))
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50 dev.off()
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51
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52 return(ltr)
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53 }
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54
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55 ltr <- do.pseudotemp(in.rdat)
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56
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57
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58 saveRDS(ltr, out.rdat)