view macros.xml @ 0:2b1797b4cbb1 draft default tip

planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spacexr commit c6f85f5bd2c9dee114640a4e4007852c060e10ca

<macros>
    <token name="@TOOL_VERSION@">2.2.1</token>
    <token name="@VERSION_SUFFIX@">0</token>
    <token name="@PROFILE@">23.0</token>
    <xml name="requirements">
        <requirements>
            <requirement type="package" version="@TOOL_VERSION@">r-spacexr</requirement>
            <yield/>
        </requirements>
    </xml>
    <xml name="edam">
        <edam_topics>
            <edam_topic>topic_3308</edam_topic>
        </edam_topics>
        <edam_operations>
            <edam_operation>operation_3223</edam_operation>
        </edam_operations>
    </xml>
    <token name="CSIDE_COMMON_RUN"><![CDATA[
#if str($type.cell_types) != '':
cell_types = c($cell_types),
#end if
cell_type_threshold = $type.cell_type_threshold,
gene_threshold = $type.gene_threshold,
doublet_mode = $type.doublet_mode,
#if str($type.weight_threshold) != '':
weight_threshold = $type.weight_threshold,
#end if
sigma_gene = $type.sigma_gene,
PRECISION.THRESHOLD = $type.precision_threshold,
#if str($type.cell_types_present) != '':
cell_types_present = c($cell_types_present),
#end if
fdr = $type.fdr,
test_genes_sig = $type.test_genes_sig,
#if 'log' in $output_selector: 
logs = TRUE,
#else:
logs = FALSE,
#end if
    ]]></token>
    <token name="CSIDE_SINGLE_RUN"><![CDATA[
myRCTD <- run.CSIDE.single(myRCTD,
                            explanatory.variable,
                            normalize_expr = $type.normalize_expr,
                            log_fc_thresh = $type.log_FC_thresh,
                            fdr_method = "BH", # default
                            medv = $type.medv,
                            CSIDE_COMMON_RUN
                            )
    ]]></token>
    <xml name="sanitizer">
        <sanitizer invalid_char="">
            <valid initial="string.ascii_letters,string.digits">
                <add value="_" />
                <add value="-" />
                <add value="," />
            </valid>
        </sanitizer>
    </xml>
    <xml name="barcodes_input" token_help="" token_label="">
        <param argument="barcodes" type="data" format="tabular" optional="false" label="@LABEL@" help="@HELP@" />
    </xml>
    <xml name="radius">
        <param argument="radius" type="integer" min="0" value="50" label="Radius" help="The radius of the exponential filter. Approximately, the distance considered to be a relevant interaction."/>
    </xml>
    <xml name="cside_common_input">
        <param argument="cell_types" type="text" optional="true" label="Cell types used for CSIDE" help="(Comma separated) If null, cell types will be chosen with aggregate occurences of at least 'cell type threshold'."/>
        <param argument="cell_type_threshold" type="integer" min="0" value="125" label="Cell type threshold" help="Min occurence of number of cells for each cell type to be used."/>
        <param argument="gene_threshold" type="float" min="0" value="0.00005" label="Gene threshold" help="Minimum average normalized expression required for selecting genes."/>
        <param argument="doublet_mode" type="boolean" truevalue="TRUE" falsevalue="FALSE" checked="true" label="Use RCTD doublet mode weights?" help="Otherwise, uses RCTD full mode weights." />
        <param argument="weight_threshold" type="float" min="0" value="" optional="true" label="Weight threshold" help="The threshold of total normalized weights across all cell types in 'cell types' per pixel to be included in the model."/>
        <param argument="sigma_gene" type="boolean" truevalue="TRUE" falsevalue="FALSE" checked="true" label="Fit gene specific overdispersion parameter?" help="If FALSE, overdispersion parameter is same across all genes." />
        <param argument="precision_threshold" type="float" min="0" value="0.05" label="Precision threshold" help="For checking for convergence, the maximum parameter change per algorithm step."/>
        <param argument="cell_types_present" type="text" optional="true" label="Cell types present" help="(Comma separeated) cell types (a superset of 'cell types') to be considered as occuring often enough to consider for gene expression contamination during the step filtering out marker genes of other cell types."/>
        <param argument="fdr" type="float" min="0" value="0.01" label="FDR" help="False discovery rate for hypothesis testing."/>
        <param argument="test_genes_sig" type="boolean" truevalue="TRUE" falsevalue="FALSE" checked="true" label="Genes will be tested for significance."/>
    </xml>
    <xml name="cside_single_input">
        <expand macro="cside_common_input"/>
        <param argument="normalize_expr" type="boolean" truevalue="TRUE" falsevalue="FALSE" checked="false" label="Constrain total gene expression to sum to 1 in each condition?"/>
        <param argument="log_FC_thresh" type="float" min="0" value="0.4" label="LogFC threshold" help="The natural log fold change cutoff for differential expression."/>
        <param argument="medv" type="float" min="0" value="0.5" label="Explanatory.variable cutoff" help="For determining if enough pixels for each cell type have explanatory-variable greater than or less than this value."/>
    </xml>
    <xml name="output">
        <section name="output" title="Output Options">
            <param name="output_selector" type="select" multiple="true" optional="true" display="checkboxes" label="Select / Deselect all">
                <option value="rds">RDS file</option>
                <option value="rscript">R script</option>
                <yield/>
            </param>
        </section>
    </xml>
    <xml name="citations">
        <citations>
            <citation type="doi">10.1038/s41587-021-00830-w</citation>
            <citation type="doi">10.1038/s41592-022-01575-3</citation>
            <citation type="bibtex">@Manual{github,
                title = {SpatialeXpressionR: Cell type identification and cell type-specific differential expression in spatial transcriptomics.},
                author = {Dylan Cable},
                url = {https://github.com/dmcable/spacexr}}
            </citation>
        </citations>
    </xml>
</macros>