annotate tn93_filter.py @ 0:ba95715078c9 draft

"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/tn93/ commit eec640a7c26b728f8175885926fe368b0756d9e5"

import argparse
import csv

from Bio import SeqIO

arguments = argparse.ArgumentParser(description='Combine alignments into a single file, adding a reference sequence as well')

arguments.add_argument('-f', '--reference', help='Reference sequence', required=True, type=str)
arguments.add_argument('-d', '--distances', help='Calculated pairwise distances', required=True, type=str)
arguments.add_argument('-r', '--reads', help='Output file for filtered reads', required=True, type=str)
arguments.add_argument('-q', '--clusters', help='Compressed clusters', required=True, type=str)
settings = arguments.parse_args()

reference_name = 'REFERENCE'
reference_seq = ''

with open(settings.reference) as seq_fh:
    for seq_record in SeqIO.parse(seq_fh, 'fasta'):
        reference_name = seq_record.name
        reference_seq = seq_record.seq
        break

with open(settings.distances) as fh:
    reader = csv.reader(fh, delimiter=',')
    next(reader)
    seqs_to_filter = set()
    for line in reader:
        if line[1] not in seqs_to_filter:
            seqs_to_filter.add(line[1])
    if reference_name in seqs_to_filter:
        seqs_to_filter.remove(reference_name)

with open(settings.reads, "a+") as fh:
    seqs_filtered = list()
    for seq_record in SeqIO.parse(settings.clusters, "fasta"):
        if seq_record.name not in seqs_to_filter:
            if seq_record.name == reference_name:
                if seq_record.name not in seqs_filtered:
                    seqs_filtered.append(seq_record.name)
                else:
                    continue
    if reference_name not in seqs_filtered:
        fh.write('\n>REFERENCE\n%s' % reference_seq)