Mercurial > repos > jjohnson > mothur_toolsuite
annotate mothur/tools/mothur/pcr.seqs.xml @ 35:95d75b35e4d2
Updated tools to use Mothur 1.33. Added some misc. fixes and updates (blast repository, tool fixes)
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date | Fri, 31 Oct 2014 15:09:32 -0400 |
parents | 49058b1f8d3f |
children | 040410b8167e |
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35
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1 <tool id="mothur_pcr_seqs" name="Pcr.seqs" version="1.26.0"> |
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2 <description>Trim sequences</description> |
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3 <command interpreter="python"> |
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4 mothur_wrapper.py |
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5 --cmd='pcr.seqs' |
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6 #import re, os.path |
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7 #set results = ["'^mothur.\S+\.logfile$:'" + $logfile.__str__] |
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8 ## adds .pcr before the last extension to the input file |
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9 #set results = $results + ["'" + $re.sub(r'(^.*)\.(.*?)',r'\1.pcr.\2',$os.path.basename($fasta.__str__)) + ":'" + $pcr_fasta.__str__] |
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10 #set results = $results + ["'" + $re.sub(r'(^.*)\.(.*?)',r'\1.pcr.scrap.\2',$os.path.basename($fasta.__str__)) + ":'" + $scrap_fasta.__str__] |
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11 --outputdir='$logfile.extra_files_path' |
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12 --fasta=$fasta |
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13 #if $name_in.__str__ != "None" and len($name_in.__str__) > 0: |
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14 --name=$name_in |
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15 #set results = $results + ["'" + $re.sub(r'(^.*)\.(.*?)',r'\1.pcr.\2',$os.path.basename($name_in.__str__)) + ":'" + $name_out.__str__] |
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16 #end if |
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17 #if $group_in.__str__ != "None" and len($group_in.__str__) > 0: |
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18 --group=$group_in |
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19 #set results = $results + ["'" + $re.sub(r'(^.*)\.(.*?)',r'\1.pcr.\2',$os.path.basename($group_in.__str__)) + ":'" + $group_out.__str__] |
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20 #end if |
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21 #if $taxonomy_in.__str__ != "None" and len($taxonomy_in.__str__) > 0: |
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22 --taxonomy=$taxonomy_in |
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23 #set results = $results + ["'" + $re.sub(r'(^.*)\.(.*?)',r'\1.pcr.\2',$os.path.basename($taxonomy_in.__str__)) + ":'" + $taxonomy_out.__str__] |
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24 #end if |
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25 #if $trim.method == 'oligos': |
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26 --oligos=$trim.oligos |
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27 --nomatch=$trim.nomatch |
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28 $trim.keepprimer |
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29 #elif $trim.method == 'reference': |
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30 --ecoli=$trim.ecoli |
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31 #elif $trim.method == 'position': |
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32 #if $trim.start.__str__ != '' and int($trim.start.__str__) > 0: |
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33 --start=$trim.start |
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34 #end if |
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35 #if $trim.end.__str__ != '' and int($trim.end.__str__) > 0: |
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36 --end=$trim.end |
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37 #end if |
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38 #end if |
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39 $keepdots |
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40 --result=#echo ','.join($results) |
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41 --processors=8 |
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42 #if $pdiffs.__str__ != '' and int($pdiffs.__str__) > 0: |
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43 --pdiffs=$pdiffs |
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44 #end if |
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45 </command> |
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46 <inputs> |
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47 <param name="fasta" type="data" format="fasta" label="fasta - Candiate Sequences"/> |
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48 |
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49 <conditional name="trim"> |
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50 <param name="method" type="select" label="Trim with an oligos file?" help=""> |
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51 <option value="oligos">oligos</option> |
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52 <option value="reference">reference sequence</option> |
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53 <option value="position">start and end positions</option> |
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54 </param> |
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55 <when value="oligos"> |
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56 <param name="oligos" type="data" format="oligos" optional="true" label="oligos - barcodes and primers" |
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57 help="a file that can contain the sequences of the forward and reverse primers and barcodes and their sample identifier. |
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58 Each line of the oligos file can start with the key words "forward", "reverse", |
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59 and "barcode" or it can start with a "#" to tell mothur to ignore that line of the oligos file. "/> |
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60 <param name="nomatch" type="select" label="nomatch - action when no primer is found" |
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61 help=""> |
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62 <option value="reject" selected="true">reject (default)</option> |
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63 <option value="keep">keep</option> |
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64 </param> |
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65 <param name="keepprimer" type="boolean" falsevalue="" truevalue="--keepprimer=true" checked="false" |
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66 label="keepprimer - keep the primer in the output sequence"/> |
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67 </when> |
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68 <when value="reference"> |
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69 <param name="ecoli" type="data" format="fasta" optional="true" label="ecoli - An aligned reference sequence for trimming" |
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70 help="The ecoli parameter is used to provide a fasta file containing a single reference sequence (e.g. for e. coli) this must be aligned. Mothur will trim to the start and end positions of the reference sequence."/> |
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71 </when> |
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72 <when value="position"> |
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73 <param name="start" type="integer" value="" optional="true" label="start - a starting position to trim to"> |
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74 <validator type="in_range" message="Starting position can't be negative" min="0"/> |
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75 </param> |
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76 <param name="end" type="integer" value="" optional="true" label="end - a ending position to trim from"> |
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77 <validator type="in_range" message="Starting position can't be negative and should be " min="0"/> |
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78 </param> |
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79 </when> |
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80 </conditional> <!-- trimtype --> |
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81 |
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82 <param name="keepdots" type="boolean" falsevalue="--keepdots=false" truevalue="" checked="true" |
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83 label="keepdots - keep the leading and trailing alignment dots in the output sequences"/> |
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84 <param name="taxonomy_in" type="data" format="seq.taxonomy" optional="true" label="taxonomy - Sequence Taxonomy"/> |
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85 <param name="name_in" type="data" format="names" optional="true" label="name - Sequence representative name list"/> |
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86 <param name="group_in" type="data" format="groups" optional="true" label="group - Group file"/> |
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87 <param name="pdiffs" type="integer" value="0" label="pdiffs - number of differences to allow in the primer (default 0)"> |
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88 <validator type="in_range" message="Number of differences can't be negative" min="0"/> |
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89 </param> |
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90 </inputs> |
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91 <outputs> |
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92 <data format="html" name="logfile" label="${tool.name} on ${on_string}: logfile" /> |
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93 <data format_source="fasta" name="pcr_fasta" label="${tool.name} on ${on_string}: pcr.fasta" /> |
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94 <data format_source="fasta" name="scrap_fasta" label="${tool.name} on ${on_string}: pcr.scrap.fasta" /> |
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95 <data format="seq.taxonomy" name="taxonomy_out" label="${tool.name} on ${on_string}: tax.summary" > |
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96 <filter>taxonomy_in != None</filter> |
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97 </data> |
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98 <data format="groups" name="group_out" label="${tool.name} on ${on_string}: " > |
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99 <filter>group_in != None</filter> |
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100 </data> |
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101 <data format="names" name="name_out" label="${tool.name} on ${on_string}: " > |
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102 <filter>name_in != None</filter> |
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103 </data> |
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104 |
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105 </outputs> |
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106 <requirements> |
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107 <requirement type="package" version="1.33">mothur</requirement> |
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108 </requirements> |
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109 <tests> |
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110 </tests> |
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111 <help> |
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112 **Mothur Overview** |
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113 |
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114 Mothur_, initiated by Dr. Patrick Schloss and his software development team |
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115 in the Department of Microbiology and Immunology at The University of Michigan, |
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116 provides bioinformatics for the microbial ecology community. |
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117 |
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118 .. _Mothur: http://www.mothur.org/wiki/Main_Page |
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119 |
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120 **Command Documenation** |
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121 |
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122 The pcr.seqs_ command assigns sequences to chosen taxonomy outline. |
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123 |
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124 .. _pcr.seqs: http://www.mothur.org/wiki/Pcr.seqs |
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125 |
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126 |
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127 </help> |
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128 </tool> |